RchiOBHm_Chr7g0187561

ATP synthase subunit O

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004768
Physical Location & Seq
Forward (+)
7354067 .. 7355682
1616 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16743

Sequence Viewer

Length: 777 bp
ATGGCCTTCGCTAATCGACTCCGATCTAGCCTCCCTCTCCAAGTCCTCGTTAGCAAGATTCTCAGATCGGAACCACTCTCCGCCGCCGCCACCCAGCGGGCTGTAGTTTGCCCTAGTCTGACAAAAAAAAATAACAACTCCGATGAGTTGTCGAGGAACTTTAATTCTATTGCCTCTAAGAAGGATGAGAAGGTGAAGTTGCCTTTGGTTTTGTTTGGGGGGTATGGAAAATATGCCTCTGCGTTGTACATTGCTGCAGCGAAAACTGATTGCTTGGACAAAGTCGAGTCTGAGATTCTCGATTTTGTTCAGTCTATCAAGAAAACTCCTGAGCTTTCTCAGTTTACCAAGGATCCGACGGTGCCTGCTCGTTCTAGAGTGAAGGCGATGGCGGAGATTTCTGAAAAGGTTGAACATTCAGATATTACAAAGAACTTCTTGGCTGTGTTGGCGGAACATGGAGGGCTAAATCACGTTGAAGCGATTGCAAAGAAATTTGTGGAGTTGACCATGGCGCATAAGGGAATAGTGGAAGTTAATGTGACTTCTGTGATTCCCCTCCCTGCTGAAGAGGAGAAAGAATTGAAGGAAACAATGCAGGAGATACTTGGACAAGGGAAGACAGTGAAGCTTGAACAGAAGATAGATCCGGGGATTCTGGGAGGGCTTGTGGTAGAGTTTCAGCAGAAGATGGTGGACATGTCCATCAAGACTAGGGCACGCCAGATGGAGAGGTACTTGCAGGATCCCAAACACTGGGGTGACCTCCTCCTCTAA

Protein Analysis

258

Amino Acids

28.64

Weight (kDa)

8.87

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OSCP PF00213 77 - 247 3.4e-43 ATP synthase delta (OSCP) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 361
AccB7I CCANNNNNTGG 1 cut(s) 756
AciI CCGC 6 cut(s) 81, 84, 87, 97, 392, 452
AclWI GGATC 5 cut(s) 347, 360, 641, 740, 753
AcsI RAATTY 1 cut(s) 494
AcuI CTGAAG 1 cut(s) 588
AfaI GTAC 2 cut(s) 248, 737
AfiI CCNNNNNNNGG 2 cut(s) 96, 756
AflIII ACRYGT 1 cut(s) 699
AgsI TTSAA 4 cut(s) 413, 479, 586, 635
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AleI CACNNNNGTG 1 cut(s) 759
AluBI AGCT 2 cut(s) 334, 631
AluI AGCT 2 cut(s) 334, 631
AlwI GGATC 5 cut(s) 347, 360, 641, 740, 753
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 254, 257
ApoI RAATTY 1 cut(s) 494
AspLEI GCGC 1 cut(s) 517
AsuC2I CCSGG 1 cut(s) 651
AsuHPI GGTGA 2 cut(s) 205, 773
BaeGI GKGCMC 1 cut(s) 721
BamHI GGATCC 2 cut(s) 352, 745
BanI GGYRCC 1 cut(s) 361
BbsI GAAGAC 1 cut(s) 626
BbvI GCAGC 2 cut(s) 241, 269
BccI CCATC 4 cut(s) 382, 685, 713, 721
BcnI CCSGG 1 cut(s) 651
BfaI CTAG 4 cut(s) 27, 114, 375, 714
BfmI CTRYAG 2 cut(s) 102, 255
BisI GCNGC 4 cut(s) 84, 87, 255, 258
BlsI GCNGC 4 cut(s) 85, 88, 256, 259
Bme1390I CCNGG 1 cut(s) 651
BmiI GGNNCC 4 cut(s) 72, 354, 363, 747
BmrFI CCNGG 1 cut(s) 651
BmrI ACTGGG 1 cut(s) 766
BmuI ACTGGG 1 cut(s) 766
BpiI GAAGAC 1 cut(s) 626
Bpu10I CCTNAGC 1 cut(s) 330
BpuMI CCSGG 1 cut(s) 651
BsaJI CCNNGG 3 cut(s) 348, 510, 650
BsaXI ACNNNNNCTCC 2 cut(s) 654, 684
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 756
Bse1I ACTGG 1 cut(s) 761
Bse3DI GCAATG 1 cut(s) 249
BseDI CCNNGG 3 cut(s) 348, 510, 650
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 2 cut(s) 96, 756
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 4 cut(s) 76, 282, 321, 353
BseNI ACTGG 1 cut(s) 761
BseRI GAGGAG 3 cut(s) 587, 758, 761
BseSI GKGCMC 1 cut(s) 721
BseXI GCAGC 2 cut(s) 241, 269
BseYI CCCAGC 1 cut(s) 93
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 361
BsiSI CCGG 1 cut(s) 650
BslI CCNNNNNNNGG 2 cut(s) 96, 756
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 721
Bsp1407I TGTACA 1 cut(s) 246
Bsp143I GATC 5 cut(s) 23, 65, 352, 646, 745
Bsp19I CCATGG 1 cut(s) 510
BspACI CCGC 6 cut(s) 81, 84, 87, 97, 392, 452
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 4 cut(s) 75, 283, 322, 352
BspLI GGNNCC 4 cut(s) 72, 354, 363, 747
BspMAI CTGCAG 1 cut(s) 259
BspPI GGATC 5 cut(s) 347, 360, 641, 740, 753
BspT107I GGYRCC 1 cut(s) 361
BsrDI GCAATG 1 cut(s) 249
BsrGI TGTACA 1 cut(s) 246
BsrI ACTGG 1 cut(s) 761
BssECI CCNNGG 3 cut(s) 348, 510, 650
BssMI GATC 5 cut(s) 23, 65, 352, 646, 745
BssT1I CCWWGG 2 cut(s) 348, 510
Bst4CI ACNGT 2 cut(s) 361, 625
Bst6I CTCTTC 1 cut(s) 564
BstAUI TGTACA 1 cut(s) 246
BstC8I GCNNGC 3 cut(s) 99, 366, 721
BstDEI CTNAG 5 cut(s) 62, 177, 291, 330, 339
BstDSI CCRYGG 1 cut(s) 510
BstEII GGTNACC 1 cut(s) 761
BstF5I GGATG 1 cut(s) 190
BstHHI GCGC 1 cut(s) 517
BstKTI GATC 5 cut(s) 26, 68, 355, 649, 748
BstMBI GATC 5 cut(s) 23, 65, 352, 646, 745
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstNSI RCATGY 1 cut(s) 703
BstPI GGTNACC 1 cut(s) 761
BstSCI CCNGG 1 cut(s) 649
BstSFI CTRYAG 2 cut(s) 102, 255
BstSLI GKGCMC 1 cut(s) 721
BstV1I GCAGC 2 cut(s) 241, 269
BstV2I GAAGAC 1 cut(s) 626
BstX2I RGATCY 3 cut(s) 352, 646, 745
BstYI RGATCY 3 cut(s) 352, 646, 745
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 510
BtgZI GCGATG 1 cut(s) 401
BtsCI GGATG 1 cut(s) 190
BtsIMutI CAGTG 2 cut(s) 630, 754
Cac8I GCNNGC 3 cut(s) 99, 366, 721
CfoI GCGC 1 cut(s) 517
Csp6I GTAC 2 cut(s) 247, 736
CviAII CATG 3 cut(s) 458, 511, 700
CviJI RGCY 8 cut(s) 5, 30, 101, 334, 443, 466, 631, 667
CviKI_1 RGCY 8 cut(s) 5, 30, 101, 334, 443, 466, 631, 667
CviQI GTAC 2 cut(s) 247, 736
DdeI CTNAG 5 cut(s) 62, 177, 291, 330, 339
DpnI GATC 5 cut(s) 25, 67, 354, 648, 747
DpnII GATC 5 cut(s) 23, 65, 352, 646, 745
Eam1104I CTCTTC 1 cut(s) 564
EarI CTCTTC 1 cut(s) 564
EciI GGCGGA 3 cut(s) 70, 407, 467
Eco130I CCWWGG 2 cut(s) 348, 510
Eco57I CTGAAG 1 cut(s) 588
Eco91I GGTNACC 1 cut(s) 761
EcoO65I GGTNACC 1 cut(s) 761
EcoT14I CCWWGG 2 cut(s) 348, 510
ErhI CCWWGG 2 cut(s) 348, 510
FaeI CATG 3 cut(s) 461, 514, 703
FaiI YATR 6 cut(s) 225, 234, 459, 512, 519, 701
FalI AAGNNNNNCTT 2 cut(s) 422, 454
FatI CATG 3 cut(s) 457, 510, 699
FauI CCCGC 1 cut(s) 90
Fnu4HI GCNGC 4 cut(s) 84, 87, 255, 258
FokI GGATG 1 cut(s) 197
Fsp4HI GCNGC 4 cut(s) 84, 87, 255, 258
FspBI CTAG 4 cut(s) 27, 114, 375, 714
GlaI GCGC 1 cut(s) 516
GluI GCNGC 4 cut(s) 84, 87, 255, 258
GsaI CCCAGC 1 cut(s) 97
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 650
HhaI GCGC 1 cut(s) 517
Hin1II CATG 3 cut(s) 461, 514, 703
Hin6I GCGC 1 cut(s) 515
HinP1I GCGC 1 cut(s) 515
HincII GTYRAC 1 cut(s) 507
HindII GTYRAC 1 cut(s) 507
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 6 cut(s) 18, 58, 287, 295, 553, 655
HpaII CCGG 1 cut(s) 650
HphI GGTGA 2 cut(s) 205, 773
Hpy166II GTNNAC 3 cut(s) 345, 507, 697
Hpy188I TCNGA 9 cut(s) 23, 65, 70, 120, 142, 292, 357, 403, 421
Hpy188III TCNNGA 5 cut(s) 299, 319, 329, 375, 709
Hpy8I GTNNAC 3 cut(s) 345, 507, 697
Hpy99I CGWCG 1 cut(s) 361
HpyAV CCTTC 5 cut(s) 16, 175, 184, 376, 580
HpyCH4III ACNGT 2 cut(s) 361, 625
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 4 cut(s) 257, 488, 598, 742
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpyF3I CTNAG 5 cut(s) 62, 177, 291, 330, 339
HpySE526I ACGT 1 cut(s) 474
Hsp92II CATG 3 cut(s) 461, 514, 703
HspAI GCGC 1 cut(s) 515
Kzo9I GATC 5 cut(s) 23, 65, 352, 646, 745
Lsp1109I GCAGC 2 cut(s) 241, 269
MaeI CTAG 4 cut(s) 27, 114, 375, 714
MaeII ACGT 1 cut(s) 474
MaeIII GTNAC 2 cut(s) 541, 761
MalI GATC 5 cut(s) 25, 67, 354, 648, 747
MboI GATC 5 cut(s) 23, 65, 352, 646, 745
MboII GAAGA 4 cut(s) 581, 631, 652, 700
MflI RGATCY 3 cut(s) 352, 646, 745
MhlI GDGCHC 1 cut(s) 721
MluCI AATT 3 cut(s) 163, 494, 581
MlyI GAGTC 2 cut(s) 12, 296
MmeI TCCRAC 1 cut(s) 380
MseI TTAA 2 cut(s) 162, 537
MslI CAYNNNNRTG 1 cut(s) 759
MspA1I CMGCKG 1 cut(s) 97
MspI CCGG 1 cut(s) 650
MspR9I CCNGG 1 cut(s) 651
MwoI GCNNNNNNNGC 1 cut(s) 449
NciI CCSGG 1 cut(s) 651
NcoI CCATGG 1 cut(s) 510
NdeII GATC 5 cut(s) 23, 65, 352, 646, 745
NlaIII CATG 3 cut(s) 461, 514, 703
NlaIV GGNNCC 4 cut(s) 72, 354, 363, 747
NmuCI GTSAC 2 cut(s) 541, 761
NspI RCATGY 1 cut(s) 703
OliI CACNNNNGTG 1 cut(s) 759
PciI ACATGT 1 cut(s) 699
PfeI GAWTC 4 cut(s) 58, 295, 553, 655
PflFI GACNNNGTC 1 cut(s) 281
PflMI CCANNNNNTGG 1 cut(s) 756
PkrI GCNGC 4 cut(s) 85, 88, 256, 259
PleI GAGTC 2 cut(s) 12, 295
PpsI GAGTC 2 cut(s) 12, 295
PscI ACATGT 1 cut(s) 699
PspEI GGTNACC 1 cut(s) 761
PspFI CCCAGC 1 cut(s) 93
PspN4I GGNNCC 4 cut(s) 72, 354, 363, 747
PstI CTGCAG 1 cut(s) 259
PsuI RGATCY 3 cut(s) 352, 646, 745
PsyI GACNNNGTC 1 cut(s) 281
RsaI GTAC 2 cut(s) 248, 737
RsaNI GTAC 2 cut(s) 247, 736
RseI CAYNNNNRTG 1 cut(s) 759
SaqAI TTAA 2 cut(s) 162, 537
SatI GCNGC 4 cut(s) 84, 87, 255, 258
Sau3AI GATC 5 cut(s) 23, 65, 352, 646, 745
SchI GAGTC 2 cut(s) 12, 296
ScrFI CCNGG 1 cut(s) 651
SduI GDGCHC 1 cut(s) 721
SetI ASST 7 cut(s) 195, 336, 411, 477, 633, 737, 768
SfcI CTRYAG 2 cut(s) 102, 255
SmiMI CAYNNNNRTG 1 cut(s) 759
Sse9I AATT 3 cut(s) 163, 494, 581
SsiI CCGC 6 cut(s) 81, 84, 87, 97, 392, 452
SspMI CTAG 4 cut(s) 27, 114, 375, 714
StyD4I CCNGG 1 cut(s) 649
StyI CCWWGG 2 cut(s) 348, 510
TaaI ACNGT 2 cut(s) 361, 625
TaiI ACGT 1 cut(s) 477
TaqI TCGA 4 cut(s) 16, 152, 285, 300
TasI AATT 3 cut(s) 163, 494, 581
TatI WGTACW 1 cut(s) 246
TauI GCSGC 2 cut(s) 86, 89
TfiI GAWTC 4 cut(s) 58, 295, 553, 655
Tru1I TTAA 2 cut(s) 162, 537
Tru9I TTAA 2 cut(s) 162, 537
TscAI CASTG 2 cut(s) 630, 761
TseFI GTSAC 2 cut(s) 541, 761
TseI GCWGC 2 cut(s) 254, 257
Tsp45I GTSAC 2 cut(s) 541, 761
TspRI CASTG 2 cut(s) 630, 761
Tth111I GACNNNGTC 1 cut(s) 281
Van91I CCANNNNNTGG 1 cut(s) 756
XapI RAATTY 1 cut(s) 494
XbaI TCTAGA 1 cut(s) 374
XceI RCATGY 1 cut(s) 703
XspI CTAG 4 cut(s) 27, 114, 375, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.