MD07G1096100.v1.1

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008433
Physical Location & Seq
Forward (+)
10473716 .. 10477063
3348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1096100.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGGCATCCGATGCTGCCATTGCCACTTGGGGCTCTGGGGAAGACGGCCAATTGGGAATCGGGAACAACGAGGAGAAGGAATGGGTGTGCGCCGTCAGAGCCCTCGAGTCGCAGACTATCCGCTCTGTTGTCGCTGGCAGCCGAAATTCTCTCGCTATTTGCGATGACGGCAAGTTGTTTACATGGGGTTGGAACCAAAGAGGAACCTTAGGGCACCCTTCCGAGACCAAAACTGAGAACATTCCAAGCCAGGTTAAAGCTCTTGCCAATGTCAAGATTGTTCAGGCTGCTATTGGTGGATGGCATTGTTTGGCTGTCGATGATCAAGGTCGTGCTTACGCTTGGGGTGGGAATGAGTATGGACAGTGTGGTGAAGAACCTGAGCGGAAAGATGACACTGTTAGACCTTTGAGAAGGGATATAGTGAATCCTCAACGCTGTGCACCAACACTTAAGGTCCGCCAGGTAGCTGCTGGGGGTACTCACTCTGTGGTACTTACACGTGATGGGCAAGTATGGACATGGGGTCAGCCATGGCCTCCGGGAGACATAAAACAAATTTCAACACCTGTGCGTGTACAAGGTCTTGATGCAGTGAGGCTCATTGCAGTTGGGGCATTTCATAATTTGGCTCTGCAGGAGGATGGTACTTTATGGGCTTGGGGTAATAACGAATATGGACAACTTGGAACAGGGGATACTCAGCCGAGATCGCAGCCTATTCCTGTCCAGGGGCTATCTGGTCTTAATTTGGTGGATATTGCTGCTGGAGGATGGCATTCTACTGCACTAACAGAAGATGGAGAGGTGTATGGTTGGGGACGAGGAGAACATGGAAGGCTTGGGTTTGGTGACAATGATAAAAGTAGTAAAATGGTGCCGCAAAGGGTTCATCTTTTAGCTGGGGAGGATATTGTTCAGGTGTCTTGTGGAGGCACTCACTCTGTTGCATTAACTCGTGACGGGCGAATCTATTCGTTTGGCCGAGGCGACCACGGACGCCTTGGATATGGAAGGAAGGTGACGACTGGTCAGCCGATGGAAGTTCCCATTGGCATCCCTCCCAGAAATGGCACTGCAGACAATGGACACTGGATTGCCAAACTCGTCGCTTCTGGAGGACGCCATACGTTGGCAATCGTAGAATGGAAAGCCGATGATGAACCAGAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1

Protein Analysis

392

Amino Acids

42.02

Weight (kDa)

5.9

Isoelectric Point (pI)

26.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alsin_RLD PF25384 6 - 112 9.6e-09 Alsin RCC1-like domain
WD40_RLD PF25390 7 - 379 2.1e-92 RCC1-like domain
RCC1_2 PF13540 40 - 68 4.7e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 56 - 106 1.7e-15 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 93 - 122 2.1e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 109 - 166 3.8e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 153 - 177 1.3e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 199 - 228 4.1e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 215 - 264 3.1e-16 Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD PF25384 217 - 283 4.8e-07 Alsin RCC1-like domain
RCC1_2 PF13540 252 - 279 3.1e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 267 - 318 7.2e-17 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 305 - 333 2.8e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 321 - 380 1.9e-08 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 213, 877
AccB7I CCANNNNNTGG 1 cut(s) 1132
AccBSI CCGCTC 2 cut(s) 123, 385
AciI CCGC 4 cut(s) 121, 385, 460, 881
AcoI YGGCCR 2 cut(s) 46, 982
AcsI RAATTY 2 cut(s) 145, 558
AcvI CACGTG 1 cut(s) 503
AcyI GRCGYC 2 cut(s) 1000, 1123
AdeI CACNNNGTG 1 cut(s) 490
AfaI GTAC 4 cut(s) 481, 495, 579, 649
AfiI CCNNNNNNNGG 3 cut(s) 731, 1070, 1132
AflII CTTAAG 1 cut(s) 452
AflIII ACRYGT 1 cut(s) 500
AgsI TTSAA 1 cut(s) 564
AhdI GACNNNNNGTC 2 cut(s) 525, 1029
AjnI CCWGG 3 cut(s) 249, 462, 729
AjuI GAANNNNNNNTTGG 2 cut(s) 1035, 1067
AluBI AGCT 3 cut(s) 260, 470, 902
AluI AGCT 3 cut(s) 260, 470, 902
Alw21I GWGCWC 1 cut(s) 445
Alw26I GTCTC 2 cut(s) 218, 540
Alw44I GTGCAC 1 cut(s) 441
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 3 cut(s) 46, 536, 982
ApaLI GTGCAC 1 cut(s) 441
ApeKI GCWGC 6 cut(s) 14, 138, 287, 470, 715, 764
ApoI RAATTY 2 cut(s) 145, 558
Asp700I GAANNNNTTC 1 cut(s) 973
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 1 cut(s) 457
AsuC2I CCSGG 1 cut(s) 543
AsuHPI GGTGA 3 cut(s) 383, 863, 1033
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 457
AxyI CCTNAGG 1 cut(s) 208
BaeGI GKGCMC 2 cut(s) 216, 445
BanI GGYRCC 2 cut(s) 213, 877
BanII GRGCYC 2 cut(s) 35, 103
BauI CACGAG 1 cut(s) 957
BbrPI CACGTG 1 cut(s) 503
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 445
BbvI GCAGC 5 cut(s) 150, 274, 457, 727, 751
BccI CCATC 6 cut(s) 294, 500, 638, 768, 794, 1033
BceAI ACGGC 3 cut(s) 61, 77, 184
BciT130I CCWGG 3 cut(s) 251, 464, 731
BciVI GTATCC 1 cut(s) 691
BclI TGATCA 1 cut(s) 322
BcnI CCSGG 1 cut(s) 543
BcoDI GTCTC 2 cut(s) 218, 540
BfmI CTRYAG 2 cut(s) 635, 1077
BfrI CTTAAG 1 cut(s) 452
BfuI GTATCC 1 cut(s) 691
BisI GCNGC 7 cut(s) 15, 139, 288, 471, 716, 765, 881
BlsI GCNGC 7 cut(s) 16, 140, 289, 472, 717, 766, 882
Bme1390I CCNGG 4 cut(s) 251, 464, 543, 731
Bme18I GGWCC 1 cut(s) 457
BmeRI GACNNNNNGTC 2 cut(s) 525, 1029
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 1 cut(s) 457
BmiI GGNNCC 4 cut(s) 194, 205, 215, 879
BmrFI CCNGG 4 cut(s) 251, 464, 543, 731
BmsI GCATC 3 cut(s) 14, 580, 1065
BpiI GAAGAC 1 cut(s) 48
BpmI CTGGAG 2 cut(s) 789, 1137
Bpu10I CCTNAGC 1 cut(s) 381
BpuMI CCSGG 1 cut(s) 543
BsaAI YACGTR 1 cut(s) 503
BsaHI GRCGYC 2 cut(s) 1000, 1123
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 5 cut(s) 533, 730, 985, 994, 1003
Bsc4I CCNNNNNNNGG 3 cut(s) 731, 1070, 1132
Bse1I ACTGG 2 cut(s) 1033, 1097
Bse21I CCTNAGG 1 cut(s) 208
Bse3DI GCAATG 2 cut(s) 18, 603
BseBI CCWGG 3 cut(s) 251, 464, 731
BseDI CCNNGG 5 cut(s) 533, 730, 985, 994, 1003
BseGI GGATG 5 cut(s) 5, 305, 649, 779, 1056
BseLI CCNNNNNNNGG 3 cut(s) 731, 1070, 1132
BseMI GCAATG 2 cut(s) 18, 603
BseMII CTCAG 3 cut(s) 225, 372, 716
BseNI ACTGG 2 cut(s) 1033, 1097
BseRI GAGGAG 2 cut(s) 86, 840
BseSI GKGCMC 2 cut(s) 216, 445
BseXI GCAGC 5 cut(s) 150, 274, 457, 727, 751
BseYI CCCAGC 2 cut(s) 473, 902
BsgI GTGCAG 1 cut(s) 771
BshFI GGCC 3 cut(s) 48, 538, 984
BshNI GGYRCC 2 cut(s) 213, 877
BsiHKAI GWGCWC 1 cut(s) 445
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 1 cut(s) 542
BslFI GGGAC 1 cut(s) 834
BslI CCNNNNNNNGG 3 cut(s) 731, 1070, 1132
BsmAI GTCTC 2 cut(s) 218, 540
BsmFI GGGAC 1 cut(s) 834
BsmI GAATGC 1 cut(s) 778
BsnI GGCC 3 cut(s) 48, 538, 984
Bso31I GGTCTC 1 cut(s) 218
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 4 cut(s) 35, 103, 216, 445
Bsp1407I TGTACA 1 cut(s) 577
Bsp143I GATC 2 cut(s) 322, 710
Bsp19I CCATGG 1 cut(s) 533
BspACI CCGC 4 cut(s) 121, 385, 460, 881
BspANI GGCC 3 cut(s) 48, 538, 984
BspCNI CTCAG 3 cut(s) 226, 373, 715
BspLI GGNNCC 4 cut(s) 194, 205, 215, 879
BspMAI CTGCAG 2 cut(s) 639, 1081
BspT107I GGYRCC 2 cut(s) 213, 877
BspTI CTTAAG 1 cut(s) 452
BspTNI GGTCTC 1 cut(s) 218
BsrBI CCGCTC 2 cut(s) 123, 385
BsrDI GCAATG 2 cut(s) 18, 603
BsrGI TGTACA 1 cut(s) 577
BsrI ACTGG 2 cut(s) 1033, 1097
BssECI CCNNGG 5 cut(s) 533, 730, 985, 994, 1003
BssMI GATC 2 cut(s) 322, 710
BssNI GRCGYC 2 cut(s) 1000, 1123
BssSI CACGAG 1 cut(s) 957
BssT1I CCWWGG 2 cut(s) 533, 1003
Bst2BI CACGAG 1 cut(s) 957
Bst2UI CCWGG 3 cut(s) 251, 464, 731
Bst4CI ACNGT 2 cut(s) 366, 400
BstACI GRCGYC 2 cut(s) 1000, 1123
BstAFI CTTAAG 1 cut(s) 452
BstAPI GCANNNNNTGC 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 577
BstBAI YACGTR 1 cut(s) 503
BstC8I GCNNGC 1 cut(s) 136
BstDEI CTNAG 4 cut(s) 208, 234, 381, 702
BstDSI CCRYGG 2 cut(s) 533, 994
BstF5I GGATG 5 cut(s) 5, 305, 649, 779, 1056
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 2 cut(s) 325, 713
BstMAI GTCTC 2 cut(s) 218, 540
BstMBI GATC 2 cut(s) 322, 710
BstMWI GCNNNNNNNGC 6 cut(s) 11, 20, 98, 168, 614, 712
BstNI CCWGG 3 cut(s) 251, 464, 731
BstSCI CCNGG 4 cut(s) 249, 462, 541, 729
BstSFI CTRYAG 2 cut(s) 635, 1077
BstSLI GKGCMC 2 cut(s) 216, 445
BstV1I GCAGC 5 cut(s) 150, 274, 457, 727, 751
BstV2I GAAGAC 1 cut(s) 48
Bsu36I CCTNAGG 1 cut(s) 208
BsuI GTATCC 1 cut(s) 691
BsuRI GGCC 3 cut(s) 48, 538, 984
BtgI CCRYGG 2 cut(s) 533, 994
BtgZI GCGATG 1 cut(s) 177
BtsCI GGATG 5 cut(s) 5, 305, 649, 779, 1056
BtsI GCAGTG 2 cut(s) 600, 1074
BtsIMutI CAGTG 5 cut(s) 371, 396, 600, 1074, 1090
Cac8I GCNNGC 1 cut(s) 136
CfoI GCGC 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 457
CseI GACGC 2 cut(s) 1008, 1131
Csp6I GTAC 4 cut(s) 480, 494, 578, 648
CviAII CATG 4 cut(s) 183, 522, 534, 833
CviQI GTAC 4 cut(s) 480, 494, 578, 648
DdeI CTNAG 4 cut(s) 208, 234, 381, 702
DpnI GATC 2 cut(s) 324, 712
DpnII GATC 2 cut(s) 322, 710
DraIII CACNNNGTG 1 cut(s) 490
DriI GACNNNNNGTC 2 cut(s) 525, 1029
EaeI YGGCCR 2 cut(s) 46, 982
Eam1105I GACNNNNNGTC 2 cut(s) 525, 1029
EciI GGCGGA 1 cut(s) 449
Eco130I CCWWGG 2 cut(s) 533, 1003
Eco24I GRGCYC 2 cut(s) 35, 103
Eco31I GGTCTC 1 cut(s) 218
Eco47I GGWCC 1 cut(s) 457
Eco72I CACGTG 1 cut(s) 503
Eco81I CCTNAGG 1 cut(s) 208
Eco88I CYCGRG 1 cut(s) 104
EcoRII CCWGG 3 cut(s) 249, 462, 729
EcoT14I CCWWGG 2 cut(s) 533, 1003
EcoT38I GRGCYC 2 cut(s) 35, 103
ErhI CCWWGG 2 cut(s) 533, 1003
FaeI CATG 4 cut(s) 186, 525, 537, 836
FaqI GGGAC 1 cut(s) 834
FatI CATG 4 cut(s) 182, 521, 533, 832
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 7 cut(s) 15, 139, 288, 471, 716, 765, 881
FokI GGATG 4 cut(s) 312, 656, 786, 1043
FriOI GRGCYC 2 cut(s) 35, 103
Fsp4HI GCNGC 7 cut(s) 15, 139, 288, 471, 716, 765, 881
GlaI GCGC 1 cut(s) 91
GluI GCNGC 7 cut(s) 15, 139, 288, 471, 716, 765, 881
GsaI CCCAGC 2 cut(s) 477, 906
GsuI CTGGAG 2 cut(s) 789, 1137
HaeIII GGCC 3 cut(s) 48, 538, 984
HapII CCGG 1 cut(s) 542
HgaI GACGC 2 cut(s) 1008, 1131
HhaI GCGC 1 cut(s) 92
Hin1I GRCGYC 2 cut(s) 1000, 1123
Hin1II CATG 4 cut(s) 186, 525, 537, 836
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HinfI GANTC 5 cut(s) 57, 107, 427, 969, 1169
HpaII CCGG 1 cut(s) 542
HphI GGTGA 3 cut(s) 383, 863, 1033
Hpy166II GTNNAC 3 cut(s) 180, 443, 578
Hpy188I TCNGA 3 cut(s) 10, 98, 223
Hpy188III TCNNGA 5 cut(s) 61, 274, 587, 959, 1116
Hpy8I GTNNAC 3 cut(s) 180, 443, 578
Hpy99I CGWCG 1 cut(s) 1112
HpyAV CCTTC 6 cut(s) 70, 228, 408, 831, 1008, 1012
HpyCH4III ACNGT 2 cut(s) 366, 400
HpyCH4IV ACGT 2 cut(s) 502, 1130
HpyCH4V TGCA 7 cut(s) 443, 593, 608, 637, 788, 950, 1079
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 20, 98, 168, 614, 712
HpyF3I CTNAG 4 cut(s) 208, 234, 381, 702
HpySE526I ACGT 2 cut(s) 502, 1130
Hsp92I GRCGYC 2 cut(s) 1000, 1123
Hsp92II CATG 4 cut(s) 186, 525, 537, 836
HspAI GCGC 1 cut(s) 90
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 2 cut(s) 322, 710
Lsp1109I GCAGC 5 cut(s) 150, 274, 457, 727, 751
LweI GCATC 3 cut(s) 14, 580, 1065
MaeII ACGT 2 cut(s) 502, 1130
MaeIII GTNAC 3 cut(s) 851, 959, 1021
MalI GATC 2 cut(s) 324, 712
MbiI CCGCTC 2 cut(s) 123, 385
MboI GATC 2 cut(s) 322, 710
MboII GAAGA 3 cut(s) 53, 386, 809
MfeI CAATTG 1 cut(s) 50
MhlI GDGCHC 4 cut(s) 35, 103, 216, 445
MluCI AATT 5 cut(s) 50, 145, 558, 625, 748
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 170
MroXI GAANNNNTTC 1 cut(s) 973
MseI TTAA 4 cut(s) 255, 453, 747, 953
MspCI CTTAAG 1 cut(s) 452
MspI CCGG 1 cut(s) 542
MspR9I CCNGG 4 cut(s) 251, 464, 543, 731
MunI CAATTG 1 cut(s) 50
Mva1269I GAATGC 1 cut(s) 778
MvaI CCWGG 3 cut(s) 251, 464, 731
MwoI GCNNNNNNNGC 6 cut(s) 11, 20, 98, 168, 614, 712
NciI CCSGG 1 cut(s) 543
NcoI CCATGG 1 cut(s) 533
NdeII GATC 2 cut(s) 322, 710
NlaIII CATG 4 cut(s) 186, 525, 537, 836
NlaIV GGNNCC 4 cut(s) 194, 205, 215, 879
NmeAIII GCCGAG 2 cut(s) 732, 1010
NmuCI GTSAC 3 cut(s) 851, 959, 1021
PaeR7I CTCGAG 1 cut(s) 104
PcsI WCGNNNNNNNCGW 3 cut(s) 66, 159, 964
PctI GAATGC 1 cut(s) 778
PdmI GAANNNNTTC 1 cut(s) 973
PfeI GAWTC 4 cut(s) 57, 427, 969, 1169
PflMI CCANNNNNTGG 1 cut(s) 1132
PfoI TCCNGGA 1 cut(s) 541
PkrI GCNGC 7 cut(s) 16, 140, 289, 472, 717, 766, 882
PleI GAGTC 1 cut(s) 115
PmaCI CACGTG 1 cut(s) 503
PmlI CACGTG 1 cut(s) 503
PpsI GAGTC 1 cut(s) 115
Ppu21I YACGTR 1 cut(s) 503
Psp6I CCWGG 3 cut(s) 249, 462, 729
PspCI CACGTG 1 cut(s) 503
PspFI CCCAGC 2 cut(s) 473, 902
PspGI CCWGG 3 cut(s) 249, 462, 729
PspN4I GGNNCC 4 cut(s) 194, 205, 215, 879
PspPI GGNCC 1 cut(s) 457
PspXI VCTCGAGB 1 cut(s) 104
PsrI GAACNNNNNNTAC 2 cut(s) 682, 714
PstI CTGCAG 2 cut(s) 639, 1081
RsaI GTAC 4 cut(s) 481, 495, 579, 649
RsaNI GTAC 4 cut(s) 480, 494, 578, 648
SaqAI TTAA 4 cut(s) 255, 453, 747, 953
SatI GCNGC 7 cut(s) 15, 139, 288, 471, 716, 765, 881
Sau3AI GATC 2 cut(s) 322, 710
Sau96I GGNCC 1 cut(s) 457
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 4 cut(s) 251, 464, 543, 731
SduI GDGCHC 4 cut(s) 35, 103, 216, 445
SfaNI GCATC 3 cut(s) 14, 580, 1065
SfcI CTRYAG 2 cut(s) 635, 1077
Sfr274I CTCGAG 1 cut(s) 104
SinI GGWCC 1 cut(s) 457
SlaI CTCGAG 1 cut(s) 104
SmlI CTYRAG 2 cut(s) 104, 452
SmoI CTYRAG 2 cut(s) 104, 452
Sse9I AATT 5 cut(s) 50, 145, 558, 625, 748
SsiI CCGC 4 cut(s) 121, 385, 460, 881
StyD4I CCNGG 4 cut(s) 249, 462, 541, 729
StyI CCWWGG 2 cut(s) 533, 1003
TaaI ACNGT 2 cut(s) 366, 400
TaiI ACGT 2 cut(s) 505, 1133
TaqI TCGA 2 cut(s) 105, 318
TasI AATT 5 cut(s) 50, 145, 558, 625, 748
TatI WGTACW 1 cut(s) 577
TauI GCSGC 1 cut(s) 883
TfiI GAWTC 4 cut(s) 57, 427, 969, 1169
Tru1I TTAA 4 cut(s) 255, 453, 747, 953
Tru9I TTAA 4 cut(s) 255, 453, 747, 953
TscAI CASTG 5 cut(s) 371, 403, 600, 1081, 1097
TseFI GTSAC 3 cut(s) 851, 959, 1021
TseI GCWGC 6 cut(s) 14, 138, 287, 470, 715, 764
Tsp45I GTSAC 3 cut(s) 851, 959, 1021
TspDTI ATGAA 3 cut(s) 611, 881, 1176
TspGWI ACGGA 1 cut(s) 1011
TspRI CASTG 5 cut(s) 371, 403, 600, 1081, 1097
Van91I CCANNNNNTGG 1 cut(s) 1132
Vha464I CTTAAG 1 cut(s) 452
VneI GTGCAC 1 cut(s) 441
VpaK11BI GGWCC 1 cut(s) 457
XapI RAATTY 2 cut(s) 145, 558
XcmI CCANNNNNNNNNTGG 3 cut(s) 470, 737, 1001
XhoI CTCGAG 1 cut(s) 104
XmnI GAANNNNTTC 1 cut(s) 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.