Rroxscaffold_2G00087570

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008433
Physical Location & Seq
Reverse (-)
9588978 .. 9593309
4332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00087570.1

Sequence Viewer

Length: 1176 bp
ATGGTATCCGATACCGCCATTATCACCTGGGGCTCCGGAGAAGATGGCCAATTGGGAATCGGGAGCAACGAGGAGAAGGACCTTGTGTGCGTTGTCACCGCCCTAGAGTCCCAGACTGTTCGCTCCGTTGTCGCCGGTAGCAGAAACTCTCTCGCCATTTGTGAAGATGGCAAGCTGTTTACATGGGGTTGGAATCAGAGAGGGACTTTGGGGCACCCTCCCGAGAGCAAAACTGAGAACGTTCCTAGCCAAGTTAAAGCTCTTGCCAATGTTAAAATCGTTCAGGCTGCTATTGGTGGATGGCATTGTTTGGCTGTTGATGATCAAGGCCGTGCTTATGCCTGGGGTGGAAATGAGTATGGACAGTGTGGTGAAGAACCTGAGCGTGACACTGGTAAACCTTTGAAAAGGGACATAGTGATACCTCAGCGCTGTGCTCCCAAACTTGTAGTTCGCCAGGTAGCTGCTGGGGGCACTCACTCTGTGGTGCTTACACGTGAAGGGCATGTATGGACATGGGGTCAACCATGGCCTCCAGGAGACATAAAACAAATTTCAACACCTGTACGAGTACAAGGTCTTGATGCAGTGAAGCTCATTGCAGTAGGGGCATTTCATAATTTGGCTCTCCAAGAGGATGGCACTTTATGGGCATGGGGTAACAACGAATATGGACAGCTTGGAACAGGGGATACGCAGCCGAGATCACAACCCATTCCTGTCCATGGACTTTCTGGTCTTACTTTGGTGGATATTGCTGCTGGAGGATGGCATTCTACTGCACTAACAGATGATGGAGAGGTGTATGGATGGGGTCGAGGGGAACATGGTAGACTTGGTTTCGGCGATAATGATAAGAGCAGTAAAATGGTCCCGCAAAAGGTTCATCTTTTAGCTGGGGAGGATATCGTTCAGGTGTCTTGTGGAGGCACTCACTCTGCTGCATTAACACGTGATGGGCGCATTTTCTCGTATGGTCGAGGTGACCATGGACGTCTCGGATACGGAAGGAACGTAACAACTGGTCAACCGATGGAAGTCCCCATCAAACTCCCTCCCAAAAATGTCAGTGACACTGAAGCCCAGGGGCATTGGATTGCCAAACTTATTGCCTCTGGAGGTCGCCATACTCTGGCAATAGTTGAATGGAAATCAGATGAATCAACTGAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1

Protein Analysis

391

Amino Acids

41.85

Weight (kDa)

5.91

Isoelectric Point (pI)

24.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alsin_RLD PF25384 4 - 112 1.2e-09 Alsin RCC1-like domain
WD40_RLD PF25390 7 - 379 1.2e-90 RCC1-like domain
RCC1 PF00415 8 - 53 1.5e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 40 - 68 4.6e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 56 - 106 1.4e-15 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 93 - 122 2.1e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 109 - 164 7.5e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 151 - 175 9.2e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 197 - 226 2.4e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 213 - 262 3.4e-15 Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD PF25384 215 - 287 1.4e-06 Alsin RCC1-like domain
RCC1_2 PF13540 250 - 277 3.5e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 265 - 316 7.9e-17 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 303 - 331 6.2e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 319 - 380 3.5e-07 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 735
AatII GACGTC 1 cut(s) 997
AccB1I GGYRCC 1 cut(s) 213
AccB7I CCANNNNNTGG 1 cut(s) 1132
AccI GTMKAC 1 cut(s) 832
AccIII TCCGGA 1 cut(s) 35
AciI CCGC 3 cut(s) 15, 99, 875
AclI AACGTT 1 cut(s) 240
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 1 cut(s) 552
AcuI CTGAAG 1 cut(s) 1098
AcvI CACGTG 2 cut(s) 497, 953
AcyI GRCGYC 1 cut(s) 994
AdeI CACNNNGTG 1 cut(s) 484
AfaI GTAC 2 cut(s) 567, 573
AfeI AGCGCT 1 cut(s) 431
AfiI CCNNNNNNNGG 3 cut(s) 725, 880, 1132
AflIII ACRYGT 2 cut(s) 494, 950
AgsI TTSAA 3 cut(s) 406, 558, 1145
AhdI GACNNNNNGTC 1 cut(s) 519
AjnI CCWGG 5 cut(s) 26, 341, 456, 535, 1083
AluBI AGCT 6 cut(s) 175, 260, 464, 595, 679, 896
AluI AGCT 6 cut(s) 175, 260, 464, 595, 679, 896
Alw21I GWGCWC 1 cut(s) 439
Alw26I GTCTC 2 cut(s) 534, 1001
Ama87I CYCGRG 1 cut(s) 221
Aor13HI TCCGGA 1 cut(s) 35
Aor51HI AGCGCT 1 cut(s) 431
AoxI GGCC 3 cut(s) 46, 328, 530
ApeKI GCWGC 5 cut(s) 287, 464, 697, 758, 941
ApoI RAATTY 1 cut(s) 552
AspLEI GCGC 2 cut(s) 432, 963
AspS9I GGNCC 2 cut(s) 79, 871
AsuHPI GGTGA 4 cut(s) 16, 88, 383, 995
AvaI CYCGRG 1 cut(s) 221
AvaII GGWCC 2 cut(s) 79, 871
BaeGI GKGCMC 2 cut(s) 216, 476
BalI TGGCCA 1 cut(s) 48
BanI GGYRCC 1 cut(s) 213
BanII GRGCYC 1 cut(s) 35
BbrPI CACGTG 2 cut(s) 497, 953
Bbv12I GWGCWC 1 cut(s) 439
BbvCI CCTCAGC 1 cut(s) 426
BbvI GCAGC 5 cut(s) 274, 451, 709, 745, 928
BceAI ACGGC 1 cut(s) 315
BciT130I CCWGG 5 cut(s) 28, 343, 458, 537, 1085
BciVI GTATCC 3 cut(s) 16, 685, 995
BclI TGATCA 1 cut(s) 322
BcoDI GTCTC 2 cut(s) 534, 1001
BfaI CTAG 2 cut(s) 104, 246
BfoI RGCGCY 1 cut(s) 433
BfuI GTATCC 3 cut(s) 16, 685, 995
BisI GCNGC 5 cut(s) 288, 465, 698, 759, 942
BlsI GCNGC 5 cut(s) 289, 466, 699, 760, 943
Bme1390I CCNGG 5 cut(s) 28, 343, 458, 537, 1085
Bme18I GGWCC 2 cut(s) 79, 871
BmeRI GACNNNNNGTC 1 cut(s) 519
BmeT110I CYCGRG 1 cut(s) 221
BmgT120I GGNCC 2 cut(s) 79, 871
BmiI GGNNCC 3 cut(s) 34, 215, 873
BmrFI CCNGG 5 cut(s) 28, 343, 458, 537, 1085
BmsI GCATC 1 cut(s) 574
BpmI CTGGAG 3 cut(s) 519, 783, 1137
Bpu10I CCTNAGC 2 cut(s) 381, 426
BsaAI YACGTR 2 cut(s) 497, 953
BsaHI GRCGYC 1 cut(s) 994
BsaJI CCNNGG 7 cut(s) 27, 342, 527, 724, 988, 1083, 1084
BsaWI WCCGGW 1 cut(s) 35
Bsc4I CCNNNNNNNGG 3 cut(s) 725, 880, 1132
Bse118I RCCGGY 1 cut(s) 134
Bse1I ACTGG 2 cut(s) 397, 1027
Bse3DI GCAATG 1 cut(s) 597
BseAI TCCGGA 1 cut(s) 35
BseBI CCWGG 5 cut(s) 28, 343, 458, 537, 1085
BseDI CCNNGG 7 cut(s) 27, 342, 527, 724, 988, 1083, 1084
BseGI GGATG 4 cut(s) 305, 643, 773, 815
BseLI CCNNNNNNNGG 3 cut(s) 725, 880, 1132
BseMI GCAATG 1 cut(s) 597
BseMII CTCAG 3 cut(s) 225, 372, 440
BseNI ACTGG 2 cut(s) 397, 1027
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 2 cut(s) 216, 476
BseXI GCAGC 5 cut(s) 274, 451, 709, 745, 928
BseYI CCCAGC 2 cut(s) 467, 896
BsgI GTGCAG 1 cut(s) 765
BshFI GGCC 3 cut(s) 48, 330, 532
BshNI GGYRCC 1 cut(s) 213
BsiHKAI GWGCWC 1 cut(s) 439
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 2 cut(s) 36, 135
BslFI GGGAC 5 cut(s) 94, 217, 425, 857, 1025
BslI CCNNNNNNNGG 3 cut(s) 725, 880, 1132
BsmAI GTCTC 2 cut(s) 534, 1001
BsmBI CGTCTC 1 cut(s) 1001
BsmFI GGGAC 5 cut(s) 94, 217, 425, 857, 1025
BsmI GAATGC 1 cut(s) 772
BsnI GGCC 3 cut(s) 48, 330, 532
BsoBI CYCGRG 1 cut(s) 221
Bsp1286I GDGCHC 4 cut(s) 35, 216, 439, 476
Bsp13I TCCGGA 1 cut(s) 35
Bsp143I GATC 2 cut(s) 322, 704
Bsp19I CCATGG 3 cut(s) 527, 724, 988
BspACI CCGC 3 cut(s) 15, 99, 875
BspANI GGCC 3 cut(s) 48, 330, 532
BspCNI CTCAG 3 cut(s) 226, 373, 439
BspEI TCCGGA 1 cut(s) 35
BspLI GGNNCC 3 cut(s) 34, 215, 873
BspT107I GGYRCC 1 cut(s) 213
BsrDI GCAATG 1 cut(s) 597
BsrFI RCCGGY 1 cut(s) 134
BsrI ACTGG 2 cut(s) 397, 1027
BssAI RCCGGY 1 cut(s) 134
BssECI CCNNGG 7 cut(s) 27, 342, 527, 724, 988, 1083, 1084
BssMI GATC 2 cut(s) 322, 704
BssNI GRCGYC 1 cut(s) 994
BssT1I CCWWGG 3 cut(s) 527, 724, 988
Bst2UI CCWGG 5 cut(s) 28, 343, 458, 537, 1085
Bst4CI ACNGT 2 cut(s) 118, 366
BstACI GRCGYC 1 cut(s) 994
BstBAI YACGTR 2 cut(s) 497, 953
BstC8I GCNNGC 1 cut(s) 173
BstDEI CTNAG 3 cut(s) 234, 381, 426
BstDSI CCRYGG 3 cut(s) 527, 724, 988
BstEII GGTNACC 1 cut(s) 983
BstF5I GGATG 4 cut(s) 305, 643, 773, 815
BstH2I RGCGCY 1 cut(s) 433
BstHHI GCGC 2 cut(s) 432, 963
BstKTI GATC 2 cut(s) 325, 707
BstMAI GTCTC 2 cut(s) 534, 1001
BstMBI GATC 2 cut(s) 322, 704
BstMWI GCNNNNNNNGC 1 cut(s) 608
BstNI CCWGG 5 cut(s) 28, 343, 458, 537, 1085
BstNSI RCATGY 1 cut(s) 509
BstPI GGTNACC 1 cut(s) 983
BstSCI CCNGG 5 cut(s) 26, 341, 456, 535, 1083
BstSLI GKGCMC 2 cut(s) 216, 476
BstV1I GCAGC 5 cut(s) 274, 451, 709, 745, 928
BstXI CCANNNNNNTGG 1 cut(s) 638
BsuI GTATCC 3 cut(s) 16, 685, 995
BsuRI GGCC 3 cut(s) 48, 330, 532
BtgI CCRYGG 3 cut(s) 527, 724, 988
BtsCI GGATG 4 cut(s) 305, 643, 773, 815
BtsI GCAGTG 1 cut(s) 594
BtsIMutI CAGTG 5 cut(s) 371, 390, 594, 1074, 1075
Cac8I GCNNGC 1 cut(s) 173
CfoI GCGC 2 cut(s) 432, 963
Cfr10I RCCGGY 1 cut(s) 134
Cfr13I GGNCC 2 cut(s) 79, 871
Csp6I GTAC 2 cut(s) 566, 572
CviAII CATG 8 cut(s) 183, 506, 516, 528, 654, 725, 827, 989
CviQI GTAC 2 cut(s) 566, 572
DdeI CTNAG 3 cut(s) 234, 381, 426
DpnI GATC 2 cut(s) 324, 706
DpnII GATC 2 cut(s) 322, 704
DraIII CACNNNGTG 1 cut(s) 484
DrdI GACNNNNNNGTC 1 cut(s) 735
DriI GACNNNNNGTC 1 cut(s) 519
DseDI GACNNNNNNGTC 1 cut(s) 735
EaeI YGGCCR 1 cut(s) 46
Eam1105I GACNNNNNGTC 1 cut(s) 519
Eco130I CCWWGG 3 cut(s) 527, 724, 988
Eco24I GRGCYC 1 cut(s) 35
Eco32I GATATC 1 cut(s) 907
Eco47I GGWCC 2 cut(s) 79, 871
Eco47III AGCGCT 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 1098
Eco72I CACGTG 2 cut(s) 497, 953
Eco88I CYCGRG 1 cut(s) 221
Eco91I GGTNACC 1 cut(s) 983
EcoO109I RGGNCCY 1 cut(s) 79
EcoO65I GGTNACC 1 cut(s) 983
EcoRII CCWGG 5 cut(s) 26, 341, 456, 535, 1083
EcoRV GATATC 1 cut(s) 907
EcoT14I CCWWGG 3 cut(s) 527, 724, 988
EcoT38I GRGCYC 1 cut(s) 35
ErhI CCWWGG 3 cut(s) 527, 724, 988
Esp3I CGTCTC 1 cut(s) 1001
FaeI CATG 8 cut(s) 186, 509, 519, 531, 657, 728, 830, 992
FaqI GGGAC 5 cut(s) 94, 217, 425, 857, 1025
FatI CATG 8 cut(s) 182, 505, 515, 527, 653, 724, 826, 988
FauI CCCGC 1 cut(s) 882
FbaI TGATCA 1 cut(s) 322
FblI GTMKAC 1 cut(s) 832
Fnu4HI GCNGC 5 cut(s) 288, 465, 698, 759, 942
FokI GGATG 4 cut(s) 312, 650, 780, 822
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 5 cut(s) 288, 465, 698, 759, 942
FspBI CTAG 2 cut(s) 104, 246
GlaI GCGC 2 cut(s) 431, 962
GluI GCNGC 5 cut(s) 288, 465, 698, 759, 942
GsaI CCCAGC 2 cut(s) 471, 900
GsuI CTGGAG 3 cut(s) 519, 783, 1137
HaeII RGCGCY 1 cut(s) 433
HaeIII GGCC 3 cut(s) 48, 330, 532
HapII CCGG 2 cut(s) 36, 135
HhaI GCGC 2 cut(s) 432, 963
Hin1I GRCGYC 1 cut(s) 994
Hin1II CATG 8 cut(s) 186, 509, 519, 531, 657, 728, 830, 992
Hin6I GCGC 2 cut(s) 430, 961
HinP1I GCGC 2 cut(s) 430, 961
HincII GTYRAC 2 cut(s) 524, 1028
HindII GTYRAC 2 cut(s) 524, 1028
HinfI GANTC 5 cut(s) 57, 107, 193, 1160, 1169
HpaII CCGG 2 cut(s) 36, 135
HphI GGTGA 4 cut(s) 16, 88, 383, 995
Hpy166II GTNNAC 5 cut(s) 180, 398, 524, 833, 1028
Hpy188I TCNGA 4 cut(s) 10, 198, 1001, 1156
Hpy188III TCNNGA 5 cut(s) 36, 61, 221, 581, 1116
Hpy8I GTNNAC 5 cut(s) 180, 398, 524, 833, 1028
HpyAV CCTTC 3 cut(s) 70, 494, 1002
HpyCH4III ACNGT 2 cut(s) 118, 366
HpyCH4IV ACGT 5 cut(s) 240, 496, 952, 994, 1014
HpyCH4V TGCA 4 cut(s) 587, 602, 782, 944
HpyF10VI GCNNNNNNNGC 1 cut(s) 608
HpyF3I CTNAG 3 cut(s) 234, 381, 426
HpySE526I ACGT 5 cut(s) 240, 496, 952, 994, 1014
Hsp92I GRCGYC 1 cut(s) 994
Hsp92II CATG 8 cut(s) 186, 509, 519, 531, 657, 728, 830, 992
HspAI GCGC 2 cut(s) 430, 961
Kpn2I TCCGGA 1 cut(s) 35
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 2 cut(s) 322, 704
LmnI GCTCC 4 cut(s) 38, 63, 128, 442
Lsp1109I GCAGC 5 cut(s) 274, 451, 709, 745, 928
LweI GCATC 1 cut(s) 574
MaeI CTAG 2 cut(s) 104, 246
MaeII ACGT 5 cut(s) 240, 496, 952, 994, 1014
MaeIII GTNAC 6 cut(s) 94, 386, 659, 983, 1015, 1070
MalI GATC 2 cut(s) 324, 706
MboI GATC 2 cut(s) 322, 704
MboII GAAGA 3 cut(s) 53, 176, 386
MfeI CAATTG 1 cut(s) 50
MhlI GDGCHC 4 cut(s) 35, 216, 439, 476
MlsI TGGCCA 1 cut(s) 48
MluCI AATT 3 cut(s) 50, 552, 619
MluNI TGGCCA 1 cut(s) 48
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 170
Mox20I TGGCCA 1 cut(s) 48
MroI TCCGGA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 48
MseI TTAA 3 cut(s) 255, 273, 947
Msp20I TGGCCA 1 cut(s) 48
MspI CCGG 2 cut(s) 36, 135
MspR9I CCNGG 5 cut(s) 28, 343, 458, 537, 1085
MunI CAATTG 1 cut(s) 50
Mva1269I GAATGC 1 cut(s) 772
MvaI CCWGG 5 cut(s) 28, 343, 458, 537, 1085
MwoI GCNNNNNNNGC 1 cut(s) 608
NcoI CCATGG 3 cut(s) 527, 724, 988
NdeII GATC 2 cut(s) 322, 704
NlaIII CATG 8 cut(s) 186, 509, 519, 531, 657, 728, 830, 992
NlaIV GGNNCC 3 cut(s) 34, 215, 873
NmeAIII GCCGAG 1 cut(s) 726
NmuCI GTSAC 4 cut(s) 94, 386, 983, 1070
NspI RCATGY 1 cut(s) 509
PasI CCCWGGG 1 cut(s) 1084
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 1011
PctI GAATGC 1 cut(s) 772
PfeI GAWTC 4 cut(s) 57, 193, 1160, 1169
PflMI CCANNNNNTGG 1 cut(s) 1132
PfoI TCCNGGA 1 cut(s) 535
PkrI GCNGC 5 cut(s) 289, 466, 699, 760, 943
PleI GAGTC 1 cut(s) 115
PmaCI CACGTG 2 cut(s) 497, 953
PmlI CACGTG 2 cut(s) 497, 953
PpsI GAGTC 1 cut(s) 115
Ppu21I YACGTR 2 cut(s) 497, 953
PpuMI RGGWCCY 1 cut(s) 79
Psp1406I AACGTT 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 79
Psp6I CCWGG 5 cut(s) 26, 341, 456, 535, 1083
PspCI CACGTG 2 cut(s) 497, 953
PspEI GGTNACC 1 cut(s) 983
PspFI CCCAGC 2 cut(s) 467, 896
PspGI CCWGG 5 cut(s) 26, 341, 456, 535, 1083
PspN4I GGNNCC 3 cut(s) 34, 215, 873
PspPI GGNCC 2 cut(s) 79, 871
PspPPI RGGWCCY 1 cut(s) 79
PsrI GAACNNNNNNTAC 2 cut(s) 676, 708
RsaI GTAC 2 cut(s) 567, 573
RsaNI GTAC 2 cut(s) 566, 572
SaqAI TTAA 3 cut(s) 255, 273, 947
SatI GCNGC 5 cut(s) 288, 465, 698, 759, 942
Sau3AI GATC 2 cut(s) 322, 704
Sau96I GGNCC 2 cut(s) 79, 871
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 5 cut(s) 28, 343, 458, 537, 1085
SduI GDGCHC 4 cut(s) 35, 216, 439, 476
SfaNI GCATC 1 cut(s) 574
SinI GGWCC 2 cut(s) 79, 871
Sse9I AATT 3 cut(s) 50, 552, 619
SsiI CCGC 3 cut(s) 15, 99, 875
SspMI CTAG 2 cut(s) 104, 246
StyD4I CCNGG 5 cut(s) 26, 341, 456, 535, 1083
StyI CCWWGG 3 cut(s) 527, 724, 988
TaaI ACNGT 2 cut(s) 118, 366
TaiI ACGT 5 cut(s) 243, 499, 955, 997, 1017
TaqI TCGA 2 cut(s) 817, 979
TasI AATT 3 cut(s) 50, 552, 619
TatI WGTACW 1 cut(s) 571
TfiI GAWTC 4 cut(s) 57, 193, 1160, 1169
Tru1I TTAA 3 cut(s) 255, 273, 947
Tru9I TTAA 3 cut(s) 255, 273, 947
TscAI CASTG 5 cut(s) 371, 397, 594, 1075, 1081
TseFI GTSAC 4 cut(s) 94, 386, 983, 1070
TseI GCWGC 5 cut(s) 287, 464, 697, 758, 941
Tsp45I GTSAC 4 cut(s) 94, 386, 983, 1070
TspDTI ATGAA 3 cut(s) 605, 875, 1173
TspGWI ACGGA 2 cut(s) 115, 1020
TspRI CASTG 5 cut(s) 371, 397, 594, 1075, 1081
Van91I CCANNNNNTGG 1 cut(s) 1132
VpaK11BI GGWCC 2 cut(s) 79, 871
XapI RAATTY 1 cut(s) 552
XceI RCATGY 1 cut(s) 509
XcmI CCANNNNNNNNNTGG 2 cut(s) 464, 731
XmiI GTMKAC 1 cut(s) 832
XspI CTAG 2 cut(s) 104, 246
ZraI GACGTC 1 cut(s) 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.