MD17G1074800.v1.1

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008433
Physical Location & Seq
Reverse (-)
6063199 .. 6066744
3546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1074800.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGGTATCCGATACCACCATTGTCACTTGGGGCTCTGGGGAAGACGGCCAATTAGGAATCGGGAATAACGAGGAGAAGGAATGGGTGTGCGCCGTCAAAGCCCTCGAGTCGCAGACTGTCCGCTCTGTTGTCGCTGGCAGCCGAAACTCCCTCGCCATTTGCGATGATGGCAAGTTGTTTACATGGGGTTGGAACCAGAGAGGAACCCTGGGGCATCCTCCTGAGACCAAATCTGAGAACATTCCAAGCCAGAGCAAAACCGAGAACATTCCAAGCCAGGTTAAAGCTCTGGCCAATGTCAAAATTGTTCAGGCTGCTATTGGTGGATGGCATTGTTTGGCTGTTGATGATCAAGGCCGCGCTTATGCCTGGGGTGGAAACGAGTATGGACAGTGTGGCGAGGAACCAGAGCGGAAAGATGAAACTAGTAGACCCTTGAGAAGGGATATAGTGATTCCTCAACGCTGTGCACCGAAACTTAAAGTTCGCCAGGTAGCTGCTGGGGGTACTCACTCTGTGGTACTTACACGCGACGGGCAAGTCTGGACGTGGGGTCAGCCATGGCCTCCGGGAGACATAAAACAAATTTCAACACCTGTGCGAGTACAAGGTCTTGATGCAGTGAGGCTTATCGCAGTTGGGGCATTTCATAATTTGGCTCTGCAAGAGGATGGTGCTTTATGGGCTTGGGGTAACAACGAATATGGACAACTTGGAACAGGGGATACTCAGCCGAGATCACAACCTATTCCTGTCCAAGGGCTTTCTGGTCTTACTTTGGTGGATATTGCTGCTGGAGGATGGCATTCTACTGCACTAACAGAAGATGGAGAGGTGTATGGTTGGGGACGAGGAGAACATGGGAGGCTTGGGTTTGGCGATAATGATAAAAGCAGCAAAATGGTGCCGCAAAAGGTTCATCTTTTAGCCGGCGAGGATATTGTTCAGGTGTCTTGTGGAGGCACTCACTCTGTTGCATTAACTCGTGATGGGCGAATGTATTCGTTTGGCCGAGGCGACCACGGACGCCTTGGATATGGAAGGAAGGTGACGACTGGTCAGCCGATGGAAGTTCCCATCGGCATCCCTCCCAGACATGGCACCGCAGACAATGGACATTGGATTGCCAAACTGGTTGCTTGCGGAGGACGCCATACCTTGGCAATCGTAGAATGGAAAGCTGATGATGAATCAGAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1

Protein Analysis

401

Amino Acids

43.08

Weight (kDa)

6.07

Isoelectric Point (pI)

31.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alsin_RLD PF25384 4 - 121 3.1e-10 Alsin RCC1-like domain
WD40_RLD PF25390 7 - 388 9e-94 RCC1-like domain
RCC1 PF00415 9 - 53 4.2e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 40 - 68 5e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 56 - 115 3.3e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 102 - 131 2.2e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 118 - 175 1.6e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 162 - 186 1.3e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 208 - 237 1.7e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 224 - 273 7.2e-16 Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD PF25384 227 - 298 3.2e-07 Alsin RCC1-like domain
RCC1_2 PF13540 261 - 288 3.2e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 276 - 327 5.8e-17 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 314 - 342 2.5e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 330 - 389 2.5e-09 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 539
AccB1I GGYRCC 2 cut(s) 904, 1100
AccB7I CCANNNNNTGG 1 cut(s) 1159
AccBSI CCGCTC 2 cut(s) 123, 412
AccI GTMKAC 1 cut(s) 430
AccII CGCG 2 cut(s) 360, 531
AciI CCGC 6 cut(s) 121, 358, 412, 908, 1104, 1143
AcoI YGGCCR 3 cut(s) 46, 291, 1009
AcsI RAATTY 1 cut(s) 585
AcyI GRCGYC 2 cut(s) 1027, 1150
AdeI CACNNNGTG 1 cut(s) 517
AfaI GTAC 3 cut(s) 508, 522, 606
AfiI CCNNNNNNNGG 4 cut(s) 441, 758, 1097, 1159
AgsI TTSAA 1 cut(s) 591
AhdI GACNNNNNGTC 2 cut(s) 552, 1056
AhlI ACTAGT 1 cut(s) 425
AjiI CACGTC 1 cut(s) 549
AjnI CCWGG 4 cut(s) 207, 276, 368, 489
AluBI AGCT 3 cut(s) 287, 497, 1181
AluI AGCT 3 cut(s) 287, 497, 1181
Alw21I GWGCWC 1 cut(s) 472
Alw26I GTCTC 2 cut(s) 218, 567
Alw44I GTGCAC 1 cut(s) 468
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 5 cut(s) 46, 291, 355, 563, 1009
ApaLI GTGCAC 1 cut(s) 468
ApeKI GCWGC 5 cut(s) 138, 314, 497, 791, 894
ApoI RAATTY 1 cut(s) 585
Asp700I GAANNNNTTC 1 cut(s) 1000
AspLEI GCGC 2 cut(s) 92, 362
AsuC2I CCSGG 1 cut(s) 570
AsuHPI GGTGA 1 cut(s) 1060
AvaI CYCGRG 1 cut(s) 104
BaeGI GKGCMC 1 cut(s) 472
BalI TGGCCA 1 cut(s) 293
BanI GGYRCC 2 cut(s) 904, 1100
BanII GRGCYC 1 cut(s) 35
BauI CACGAG 1 cut(s) 984
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 472
BbvI GCAGC 5 cut(s) 150, 301, 484, 778, 906
BccI CCATC 8 cut(s) 161, 321, 665, 795, 821, 983, 1060, 1085
BceAI ACGGC 2 cut(s) 61, 77
BciT130I CCWGG 4 cut(s) 209, 278, 370, 491
BciVI GTATCC 2 cut(s) 16, 718
BclI TGATCA 1 cut(s) 349
BcnI CCSGG 1 cut(s) 570
BcoDI GTCTC 2 cut(s) 218, 567
BcuI ACTAGT 1 cut(s) 425
BfaI CTAG 1 cut(s) 426
BfuI GTATCC 2 cut(s) 16, 718
BisI GCNGC 7 cut(s) 139, 315, 358, 498, 792, 895, 908
BlsI GCNGC 7 cut(s) 140, 316, 359, 499, 793, 896, 909
Bme1390I CCNGG 5 cut(s) 209, 278, 370, 491, 570
BmeRI GACNNNNNGTC 2 cut(s) 552, 1056
BmeT110I CYCGRG 1 cut(s) 104
BmgBI CACGTC 1 cut(s) 549
BmiI GGNNCC 5 cut(s) 194, 205, 405, 906, 1102
BmrFI CCNGG 5 cut(s) 209, 278, 370, 491, 570
BmsI GCATC 3 cut(s) 223, 607, 1092
BpiI GAAGAC 1 cut(s) 48
BpmI CTGGAG 1 cut(s) 816
BpuEI CTTGAG 1 cut(s) 457
BpuMI CCSGG 1 cut(s) 570
BsaHI GRCGYC 2 cut(s) 1027, 1150
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 9 cut(s) 207, 208, 369, 560, 757, 1012, 1021, 1030, 1158
Bsc4I CCNNNNNNNGG 4 cut(s) 441, 758, 1097, 1159
Bse118I RCCGGY 1 cut(s) 929
Bse1I ACTGG 2 cut(s) 1060, 1137
BseBI CCWGG 4 cut(s) 209, 278, 370, 491
BseDI CCNNGG 9 cut(s) 207, 208, 369, 560, 757, 1012, 1021, 1030, 1158
BseGI GGATG 5 cut(s) 214, 332, 676, 806, 1083
BseLI CCNNNNNNNGG 4 cut(s) 441, 758, 1097, 1159
BseMII CTCAG 3 cut(s) 213, 225, 743
BseNI ACTGG 2 cut(s) 1060, 1137
BseRI GAGGAG 2 cut(s) 86, 867
BseSI GKGCMC 1 cut(s) 472
BseXI GCAGC 5 cut(s) 150, 301, 484, 778, 906
BseYI CCCAGC 1 cut(s) 500
BsgI GTGCAG 1 cut(s) 798
Bsh1236I CGCG 2 cut(s) 360, 531
BshFI GGCC 5 cut(s) 48, 293, 357, 565, 1011
BshNI GGYRCC 2 cut(s) 904, 1100
BsiHKAI GWGCWC 1 cut(s) 472
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 2 cut(s) 569, 930
BslFI GGGAC 1 cut(s) 861
BslI CCNNNNNNNGG 4 cut(s) 441, 758, 1097, 1159
BsmAI GTCTC 2 cut(s) 218, 567
BsmFI GGGAC 1 cut(s) 861
BsmI GAATGC 1 cut(s) 805
BsnI GGCC 5 cut(s) 48, 293, 357, 565, 1011
Bso31I GGTCTC 1 cut(s) 218
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 2 cut(s) 35, 472
Bsp143I GATC 2 cut(s) 349, 737
Bsp19I CCATGG 1 cut(s) 560
BspACI CCGC 6 cut(s) 121, 358, 412, 908, 1104, 1143
BspANI GGCC 5 cut(s) 48, 293, 357, 565, 1011
BspCNI CTCAG 3 cut(s) 214, 226, 742
BspFNI CGCG 2 cut(s) 360, 531
BspLI GGNNCC 5 cut(s) 194, 205, 405, 906, 1102
BspT107I GGYRCC 2 cut(s) 904, 1100
BspTNI GGTCTC 1 cut(s) 218
BsrBI CCGCTC 2 cut(s) 123, 412
BsrFI RCCGGY 1 cut(s) 929
BsrI ACTGG 2 cut(s) 1060, 1137
BssAI RCCGGY 1 cut(s) 929
BssECI CCNNGG 9 cut(s) 207, 208, 369, 560, 757, 1012, 1021, 1030, 1158
BssMI GATC 2 cut(s) 349, 737
BssNI GRCGYC 2 cut(s) 1027, 1150
BssSI CACGAG 1 cut(s) 984
BssT1I CCWWGG 4 cut(s) 560, 757, 1030, 1158
Bst2BI CACGAG 1 cut(s) 984
Bst2UI CCWGG 4 cut(s) 209, 278, 370, 491
Bst4CI ACNGT 2 cut(s) 118, 393
BstACI GRCGYC 2 cut(s) 1027, 1150
BstC8I GCNNGC 3 cut(s) 136, 931, 1141
BstDEI CTNAG 3 cut(s) 222, 234, 729
BstDSI CCRYGG 2 cut(s) 560, 1021
BstENI CCTNNNNNAGG 2 cut(s) 439, 756
BstF5I GGATG 5 cut(s) 214, 332, 676, 806, 1083
BstFNI CGCG 2 cut(s) 360, 531
BstHHI GCGC 2 cut(s) 92, 362
BstKTI GATC 2 cut(s) 352, 740
BstMAI GTCTC 2 cut(s) 218, 567
BstMBI GATC 2 cut(s) 349, 737
BstMWI GCNNNNNNNGC 5 cut(s) 98, 168, 641, 683, 1149
BstNI CCWGG 4 cut(s) 209, 278, 370, 491
BstSCI CCNGG 5 cut(s) 207, 276, 368, 489, 568
BstSLI GKGCMC 1 cut(s) 472
BstUI CGCG 2 cut(s) 360, 531
BstV1I GCAGC 5 cut(s) 150, 301, 484, 778, 906
BstV2I GAAGAC 1 cut(s) 48
BsuI GTATCC 2 cut(s) 16, 718
BsuRI GGCC 5 cut(s) 48, 293, 357, 565, 1011
BtgI CCRYGG 2 cut(s) 560, 1021
BtgZI GCGATG 1 cut(s) 177
BtrI CACGTC 1 cut(s) 549
BtsCI GGATG 5 cut(s) 214, 332, 676, 806, 1083
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 2 cut(s) 398, 627
Cac8I GCNNGC 3 cut(s) 136, 931, 1141
CfoI GCGC 2 cut(s) 92, 362
Cfr10I RCCGGY 1 cut(s) 929
CseI GACGC 2 cut(s) 1035, 1158
Csp6I GTAC 3 cut(s) 507, 521, 605
CviAII CATG 4 cut(s) 183, 561, 860, 1097
CviQI GTAC 3 cut(s) 507, 521, 605
DdeI CTNAG 3 cut(s) 222, 234, 729
DpnI GATC 2 cut(s) 351, 739
DpnII GATC 2 cut(s) 349, 737
DraIII CACNNNGTG 1 cut(s) 517
DrdI GACNNNNNNGTC 1 cut(s) 539
DriI GACNNNNNGTC 2 cut(s) 552, 1056
DseDI GACNNNNNNGTC 1 cut(s) 539
EaeI YGGCCR 3 cut(s) 46, 291, 1009
Eam1105I GACNNNNNGTC 2 cut(s) 552, 1056
Eco130I CCWWGG 4 cut(s) 560, 757, 1030, 1158
Eco24I GRGCYC 1 cut(s) 35
Eco31I GGTCTC 1 cut(s) 218
Eco88I CYCGRG 1 cut(s) 104
EcoNI CCTNNNNNAGG 2 cut(s) 439, 756
EcoRII CCWGG 4 cut(s) 207, 276, 368, 489
EcoT14I CCWWGG 4 cut(s) 560, 757, 1030, 1158
EcoT38I GRGCYC 1 cut(s) 35
ErhI CCWWGG 4 cut(s) 560, 757, 1030, 1158
FaeI CATG 4 cut(s) 186, 564, 863, 1100
FaqI GGGAC 1 cut(s) 861
FatI CATG 4 cut(s) 182, 560, 859, 1096
FbaI TGATCA 1 cut(s) 349
FblI GTMKAC 1 cut(s) 430
Fnu4HI GCNGC 7 cut(s) 139, 315, 358, 498, 792, 895, 908
FokI GGATG 5 cut(s) 201, 339, 683, 813, 1070
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 7 cut(s) 139, 315, 358, 498, 792, 895, 908
FspBI CTAG 1 cut(s) 426
GlaI GCGC 2 cut(s) 91, 361
GluI GCNGC 7 cut(s) 139, 315, 358, 498, 792, 895, 908
GsaI CCCAGC 1 cut(s) 504
GsuI CTGGAG 1 cut(s) 816
HaeIII GGCC 5 cut(s) 48, 293, 357, 565, 1011
HapII CCGG 2 cut(s) 569, 930
HgaI GACGC 2 cut(s) 1035, 1158
HhaI GCGC 2 cut(s) 92, 362
Hin1I GRCGYC 2 cut(s) 1027, 1150
Hin1II CATG 4 cut(s) 186, 564, 863, 1100
Hin6I GCGC 2 cut(s) 90, 360
HinP1I GCGC 2 cut(s) 90, 360
HinfI GANTC 5 cut(s) 57, 107, 454, 1190, 1196
HpaII CCGG 2 cut(s) 569, 930
HphI GGTGA 1 cut(s) 1060
Hpy166II GTNNAC 3 cut(s) 180, 431, 470
Hpy188I TCNGA 3 cut(s) 10, 235, 1195
Hpy188III TCNNGA 5 cut(s) 61, 221, 544, 614, 986
Hpy8I GTNNAC 3 cut(s) 180, 431, 470
Hpy99I CGWCG 1 cut(s) 536
HpyAV CCTTC 4 cut(s) 70, 435, 1035, 1039
HpyCH4III ACNGT 2 cut(s) 118, 393
HpyCH4IV ACGT 1 cut(s) 548
HpyCH4V TGCA 5 cut(s) 470, 620, 664, 815, 977
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 168, 641, 683, 1149
HpyF3I CTNAG 3 cut(s) 222, 234, 729
HpySE526I ACGT 1 cut(s) 548
Hsp92I GRCGYC 2 cut(s) 1027, 1150
Hsp92II CATG 4 cut(s) 186, 564, 863, 1100
HspAI GCGC 2 cut(s) 90, 360
KroI GCCGGC 1 cut(s) 929
KroNI GCCGGC 1 cut(s) 931
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 2 cut(s) 349, 737
Lsp1109I GCAGC 5 cut(s) 150, 301, 484, 778, 906
LweI GCATC 3 cut(s) 223, 607, 1092
MaeI CTAG 1 cut(s) 426
MaeII ACGT 1 cut(s) 548
MaeIII GTNAC 3 cut(s) 22, 692, 1048
MalI GATC 2 cut(s) 351, 739
MbiI CCGCTC 2 cut(s) 123, 412
MboI GATC 2 cut(s) 349, 737
MboII GAAGA 2 cut(s) 53, 836
MhlI GDGCHC 2 cut(s) 35, 472
MlsI TGGCCA 1 cut(s) 293
MluCI AATT 4 cut(s) 50, 303, 585, 652
MluNI TGGCCA 1 cut(s) 293
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 170
Mox20I TGGCCA 1 cut(s) 293
MroNI GCCGGC 1 cut(s) 929
MroXI GAANNNNTTC 1 cut(s) 1000
MscI TGGCCA 1 cut(s) 293
MseI TTAA 3 cut(s) 282, 480, 980
Msp20I TGGCCA 1 cut(s) 293
MspI CCGG 2 cut(s) 569, 930
MspR9I CCNGG 5 cut(s) 209, 278, 370, 491, 570
Mva1269I GAATGC 1 cut(s) 805
MvaI CCWGG 4 cut(s) 209, 278, 370, 491
MvnI CGCG 2 cut(s) 360, 531
MwoI GCNNNNNNNGC 5 cut(s) 98, 168, 641, 683, 1149
NaeI GCCGGC 1 cut(s) 931
NciI CCSGG 1 cut(s) 570
NcoI CCATGG 1 cut(s) 560
NdeII GATC 2 cut(s) 349, 737
NgoMIV GCCGGC 1 cut(s) 929
NlaIII CATG 4 cut(s) 186, 564, 863, 1100
NlaIV GGNNCC 5 cut(s) 194, 205, 405, 906, 1102
NmeAIII GCCGAG 2 cut(s) 759, 1037
NmuCI GTSAC 2 cut(s) 22, 1048
PaeR7I CTCGAG 1 cut(s) 104
PasI CCCWGGG 1 cut(s) 208
PcsI WCGNNNNNNNCGW 3 cut(s) 66, 159, 991
PctI GAATGC 1 cut(s) 805
PdiI GCCGGC 1 cut(s) 931
PdmI GAANNNNTTC 1 cut(s) 1000
PfeI GAWTC 4 cut(s) 57, 454, 1190, 1196
PflMI CCANNNNNTGG 1 cut(s) 1159
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 7 cut(s) 140, 316, 359, 499, 793, 896, 909
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
Psp6I CCWGG 4 cut(s) 207, 276, 368, 489
PspFI CCCAGC 1 cut(s) 500
PspGI CCWGG 4 cut(s) 207, 276, 368, 489
PspN4I GGNNCC 5 cut(s) 194, 205, 405, 906, 1102
PspXI VCTCGAGB 1 cut(s) 104
PsrI GAACNNNNNNTAC 2 cut(s) 709, 741
RsaI GTAC 3 cut(s) 508, 522, 606
RsaNI GTAC 3 cut(s) 507, 521, 605
SaqAI TTAA 3 cut(s) 282, 480, 980
SatI GCNGC 7 cut(s) 139, 315, 358, 498, 792, 895, 908
Sau3AI GATC 2 cut(s) 349, 737
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 5 cut(s) 209, 278, 370, 491, 570
SduI GDGCHC 2 cut(s) 35, 472
SfaNI GCATC 3 cut(s) 223, 607, 1092
Sfr274I CTCGAG 1 cut(s) 104
SlaI CTCGAG 1 cut(s) 104
SmlI CTYRAG 2 cut(s) 104, 436
SmoI CTYRAG 2 cut(s) 104, 436
SpeI ACTAGT 1 cut(s) 425
Sse9I AATT 4 cut(s) 50, 303, 585, 652
SsiI CCGC 6 cut(s) 121, 358, 412, 908, 1104, 1143
SspMI CTAG 1 cut(s) 426
StyD4I CCNGG 5 cut(s) 207, 276, 368, 489, 568
StyI CCWWGG 4 cut(s) 560, 757, 1030, 1158
TaaI ACNGT 2 cut(s) 118, 393
TaiI ACGT 1 cut(s) 551
TaqI TCGA 1 cut(s) 105
TasI AATT 4 cut(s) 50, 303, 585, 652
TatI WGTACW 1 cut(s) 604
TauI GCSGC 2 cut(s) 360, 910
TfiI GAWTC 4 cut(s) 57, 454, 1190, 1196
Tru1I TTAA 3 cut(s) 282, 480, 980
Tru9I TTAA 3 cut(s) 282, 480, 980
TscAI CASTG 2 cut(s) 398, 627
TseFI GTSAC 2 cut(s) 22, 1048
TseI GCWGC 5 cut(s) 138, 314, 497, 791, 894
Tsp45I GTSAC 2 cut(s) 22, 1048
TspDTI ATGAA 4 cut(s) 435, 638, 908, 1203
TspGWI ACGGA 1 cut(s) 1038
TspRI CASTG 2 cut(s) 398, 627
Van91I CCANNNNNTGG 1 cut(s) 1159
VneI GTGCAC 1 cut(s) 468
XagI CCTNNNNNAGG 2 cut(s) 439, 756
XapI RAATTY 1 cut(s) 585
XcmI CCANNNNNNNNNTGG 3 cut(s) 497, 764, 1028
XhoI CTCGAG 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 430
XmnI GAANNNNTTC 1 cut(s) 1000
XspI CTAG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.