Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0004184

Physical Location & Seq

Genomic Coordinates Reverse (-)

12781623 .. 12787888

Gene Structure 6266 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1111300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1230 bp

ATGCAGAGGTTTAAGGCAGCTTTATCGGCCGGAAACGGCGTCCACCTCCGTCGTTTCGTCCGCCATTGCAGAGGCGTATGCTCTCTCCCGGACGCCGCTCTCACCCCAGCTGATAATCTCGATCACCAAGTGTTGGTCGAAGGAAAAGCGTGGTCCCGAACGGCAGTTCTCAACAGACCAGCGGTGCTCAATGCTTTCACGACTGCAATGGGGGCAAGATTGCAGAAATTGTACAAAAGCTGGGAAGATGATCCTGATGTTGGCTTTATTGTTTTGAAGGGCAGTGGCAAGGCATTTTGTGCCGGTGGAGATATAGTTTCTCTTTATCATATGATAAACAGAGGGAAAATAGACGATTGTAAAGATTTTTTTAGGGCGTGCTATACTTTTATATACCTGCTTGGTACCTGTTTGAAACCACATGTGGCTATCTTGAATGGCATTACCATGGGTGGTGGTGCTGGAGTCTCAATCCCTGGGACATTCCGCATTGCAACTGACAAAACTGTTTTTGCTACTCCTGAAACTCTAATTGGTTTCCATACAGATGCAGGGGCATCTTTTTACCTCTCACATCTACCGGGTCACTTAGGGGAGTTCTTGGCTCTTACAGGAGACAGGCTCAATGGGTCGGAGATGATGGCTTCTGGACTTGCAACACATTATTTGCATAGTTCAAGGCTTCCATTAATTGAAGAAGAACTTGGGAAAATTGTCACTGATGATCCCTCTGTCGTTGAAGCTTCTTTAGACAAGTATGGTGAAGTTGTTTATCCCAATGAGACAAGCGTGCTTCAAAGGATTGAGTTGCTCGATAAATGTTTCAGCCATGACACAGTTGAAGAAATTATCGATTCTGTGGAATGTGAGGCAGGTAGAACAAAGGATGCATGGTGCATTTCTACTCTAAAGAGACTTAAAGAAGTCTCACCATTGAGCTTAAAGGTTTCCTTGAGATCTATAAGAGAAGGTAGATTTCAGACCCTTGATCAGTGCTTGGTTCGTGAGTACCGAATGTCCCTACAGGGAATCACTAAGCAGATTTCCAATGACTTTTGCGAGGGGGTTCGGGCACGAGTAGTGGAAAAGGACTTTGCACCAAAGTGGGATCCTCCAAGTGTTGAAAATGTATCCAAAGACATGGTGGATCAATATTTCTCCCCTCTTAGCGAGTTCGAGCCCGACCTAGAGCTACCGACGGAGTTGCGAGAAGCGTTCACCCAGTCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200

Pfam Domains

Protein Families

ECH_2

Protein Analysis

410

Amino Acids

45.28

Weight (kDa)

5.72

Isoelectric Point (pI)

31.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_2	PF16113	53 - 386	6.1e-118	Enoyl-CoA hydratase/isomerase
ECH_1	PF00378	57 - 222	2.8e-24	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G60510 AT3G60510 AT3G60510
fragaria_vesca	FvH4_7g10610 FvH4_7g10610 FvH4_7g10610 FvH4_7g10610 FvH4_7g10610 FvH4_7g10610 FvH4_7g10610 FvH4_7g10610
malus_domestica	MD07G1111300.v1.1
prunus_persica	Prupe.2G134700_v2.0.a1 Prupe.2G134700_v2.0.a1 Prupe.2G134700_v2.0.a1 Prupe.2G134700_v2.0.a1 Prupe.2G134700_v2.0.a1
pyrus_communis	pycom07g09610
rosa_chinensis	RchiOBHm_Chr1g0348451 RchiOBHm_Chr1g0348531
rosa_laevigata	RLG00000028641 RLG00000028649 RLG00000028657
rosa_multiflora	Rmu_sc0000091.1_g000013
rosa_roxburghii	Rroxscaffold_4G00306970
rosa_rugosa	Rorug01G0194700 Rorug01G0194800
rosa_samantha	Rh1AG212800 Rh1BG179200 Rh1CG197100 Rh1DG208900 Rh1DG209300
rosa_wichuraiana	Rw1G018010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	405, 863
Acc65I	GGTACC	1 cut(s)	404
AccB1I	GGYRCC	1 cut(s)	404
AccB7I	CCANNNNNTGG	1 cut(s)	133
AccBSI	CCGCTC	1 cut(s)	98
AciI	CCGC	4 cut(s)	61, 96, 182, 487
AclWI	GGATC	5 cut(s)	245, 719, 1103, 1116, 1155
AcoI	YGGCCR	1 cut(s)	27
AcyI	GRCGYC	2 cut(s)	39, 93
AdeI	CACNNNGTG	1 cut(s)	130
AfaI	GTAC	3 cut(s)	233, 406, 1010
AfiI	CCNNNNNNNGG	2 cut(s)	133, 260
AflIII	ACRYGT	1 cut(s)	421
AgsI	TTSAA	9 cut(s)	277, 415, 436, 678, 695, 740, 797, 842, 1124
AjnI	CCWGG	1 cut(s)	475
AjuI	GAANNNNNNNTTGG	4 cut(s)	516, 548, 687, 719
AleI	CACNNNNGTG	1 cut(s)	1102
AluBI	AGCT	6 cut(s)	20, 110, 240, 743, 939, 1192
AluI	AGCT	6 cut(s)	20, 110, 240, 743, 939, 1192
Alw21I	GWGCWC	1 cut(s)	189
Alw26I	GTCTC	5 cut(s)	472, 609, 776, 907, 931
AlwI	GGATC	5 cut(s)	245, 719, 1103, 1116, 1155
AoxI	GGCC	1 cut(s)	27
ApeKI	GCWGC	1 cut(s)	17
AseI	ATTAAT	1 cut(s)	689
Asp718I	GGTACC	1 cut(s)	404
AspS9I	GGNCC	1 cut(s)	153
AsuC2I	CCSGG	2 cut(s)	89, 582
AsuHPI	GGTGA	5 cut(s)	94, 116, 773, 921, 1210
AvaII	GGWCC	1 cut(s)	153
BaeGI	GKGCMC	1 cut(s)	1075
BamHI	GGATCC	1 cut(s)	1108
BanI	GGYRCC	1 cut(s)	404
BanII	GRGCYC	1 cut(s)	1182
BauI	CACGAG	1 cut(s)	1074
Bbv12I	GWGCWC	1 cut(s)	189
BbvI	GCAGC	1 cut(s)	29
BccI	CCATC	1 cut(s)	634
BceAI	ACGGC	2 cut(s)	52, 177
BcgI	CGANNNNNNTGC	4 cut(s)	6, 40, 1186, 1220
BciT130I	CCWGG	1 cut(s)	477
BciVI	GTATCC	1 cut(s)	1141
BclI	TGATCA	1 cut(s)	988
BcnI	CCSGG	2 cut(s)	89, 582
BcoDI	GTCTC	5 cut(s)	472, 609, 776, 907, 931
BfaI	CTAG	2 cut(s)	1187, 1228
BfmI	CTRYAG	1 cut(s)	1022
BfuAI	ACCTGC	2 cut(s)	405, 863
BfuI	GTATCC	1 cut(s)	1141
BglII	AGATCT	1 cut(s)	956
BisI	GCNGC	2 cut(s)	18, 96
BlsI	GCNGC	2 cut(s)	19, 97
Bme1390I	CCNGG	3 cut(s)	89, 477, 582
Bme18I	GGWCC	1 cut(s)	153
BmgT120I	GGNCC	1 cut(s)	153
BmiI	GGNNCC	3 cut(s)	155, 406, 1110
BmrFI	CCNGG	3 cut(s)	89, 477, 582
BmrI	ACTGGG	1 cut(s)	1216
BmsI	GCATC	3 cut(s)	538, 566, 877
BmuI	ACTGGG	1 cut(s)	1216
BplI	GAGNNNNNCTC	2 cut(s)	606, 638
BpmI	CTGGAG	1 cut(s)	483
BpuEI	CTTGAG	1 cut(s)	973
BpuMI	CCSGG	2 cut(s)	89, 582
Bsa29I	ATCGAT	1 cut(s)	852
BsaHI	GRCGYC	2 cut(s)	39, 93
BsaJI	CCNNGG	3 cut(s)	447, 475, 476
BsaXI	ACNNNNNCTCC	2 cut(s)	300, 330
Bsc4I	CCNNNNNNNGG	2 cut(s)	133, 260
Bse118I	RCCGGY	1 cut(s)	302
Bse1I	ACTGG	1 cut(s)	1222
Bse3DI	GCAATG	3 cut(s)	64, 213, 489
BseBI	CCWGG	1 cut(s)	477
BseCI	ATCGAT	1 cut(s)	852
BseDI	CCNNGG	3 cut(s)	447, 475, 476
BseGI	GGATG	1 cut(s)	892
BseLI	CCNNNNNNNGG	2 cut(s)	133, 260
BseMI	GCAATG	3 cut(s)	64, 213, 489
BseNI	ACTGG	1 cut(s)	1222
BseSI	GKGCMC	1 cut(s)	1075
BseX3I	CGGCCG	1 cut(s)	27
BseXI	GCAGC	1 cut(s)	29
BseYI	CCCAGC	2 cut(s)	106, 240
Bsh1285I	CGRYCG	1 cut(s)	30
BshFI	GGCC	1 cut(s)	29
BshNI	GGYRCC	1 cut(s)	404
BshVI	ATCGAT	1 cut(s)	852
BsiEI	CGRYCG	1 cut(s)	30
BsiHKAI	GWGCWC	1 cut(s)	189
BsiSI	CCGG	4 cut(s)	30, 89, 303, 581
BslFI	GGGAC	3 cut(s)	139, 493, 1003
BslI	CCNNNNNNNGG	2 cut(s)	133, 260
BsmAI	GTCTC	5 cut(s)	472, 609, 776, 907, 931
BsmFI	GGGAC	3 cut(s)	139, 493, 1003
BsnI	GGCC	1 cut(s)	29
Bsp1286I	GDGCHC	3 cut(s)	189, 1075, 1182
Bsp1407I	TGTACA	1 cut(s)	231
Bsp143I	GATC	7 cut(s)	121, 250, 724, 956, 988, 1108, 1147
Bsp19I	CCATGG	1 cut(s)	447
BspACI	CCGC	4 cut(s)	61, 96, 182, 487
BspANI	GGCC	1 cut(s)	29
BspDI	ATCGAT	1 cut(s)	852
BspLI	GGNNCC	3 cut(s)	155, 406, 1110
BspMI	ACCTGC	2 cut(s)	405, 863
BspPI	GGATC	5 cut(s)	245, 719, 1103, 1116, 1155
BspT107I	GGYRCC	1 cut(s)	404
BsrBI	CCGCTC	1 cut(s)	98
BsrDI	GCAATG	3 cut(s)	64, 213, 489
BsrFI	RCCGGY	1 cut(s)	302
BsrGI	TGTACA	1 cut(s)	231
BsrI	ACTGG	1 cut(s)	1222
BssAI	RCCGGY	1 cut(s)	302
BssECI	CCNNGG	3 cut(s)	447, 475, 476
BssMI	GATC	7 cut(s)	121, 250, 724, 956, 988, 1108, 1147
BssNI	GRCGYC	2 cut(s)	39, 93
BssSI	CACGAG	1 cut(s)	1074
BssT1I	CCWWGG	1 cut(s)	447
Bst2BI	CACGAG	1 cut(s)	1074
Bst2UI	CCWGG	1 cut(s)	477
Bst4CI	ACNGT	2 cut(s)	508, 838
BstACI	GRCGYC	2 cut(s)	39, 93
BstAPI	GCANNNNNTGC	1 cut(s)	299
BstAUI	TGTACA	1 cut(s)	231
BstC8I	GCNNGC	2 cut(s)	379, 791
BstDEI	CTNAG	3 cut(s)	589, 1035, 1166
BstDSI	CCRYGG	1 cut(s)	447
BstF5I	GGATG	1 cut(s)	892
BstKTI	GATC	7 cut(s)	124, 253, 727, 959, 991, 1111, 1150
BstMAI	GTCTC	5 cut(s)	472, 609, 776, 907, 931
BstMBI	GATC	7 cut(s)	121, 250, 724, 956, 988, 1108, 1147
BstMCI	CGRYCG	1 cut(s)	30
BstMWI	GCNNNNNNNGC	3 cut(s)	26, 212, 299
BstNI	CCWGG	1 cut(s)	477
BstNSI	RCATGY	1 cut(s)	425
BstSCI	CCNGG	3 cut(s)	87, 475, 580
BstSFI	CTRYAG	1 cut(s)	1022
BstSLI	GKGCMC	1 cut(s)	1075
BstV1I	GCAGC	1 cut(s)	29
BstX2I	RGATCY	2 cut(s)	956, 1108
BstXI	CCANNNNNNTGG	1 cut(s)	1141
BstYI	RGATCY	2 cut(s)	956, 1108
BstZI	CGGCCG	1 cut(s)	27
Bsu15I	ATCGAT	1 cut(s)	852
BsuI	GTATCC	1 cut(s)	1141
BsuRI	GGCC	1 cut(s)	29
BsuTUI	ATCGAT	1 cut(s)	852
BtgI	CCRYGG	1 cut(s)	447
BtsCI	GGATG	1 cut(s)	892
BtsI	GCAGTG	1 cut(s)	289
BtsIMutI	CAGTG	3 cut(s)	289, 717, 998
BveI	ACCTGC	2 cut(s)	405, 863
Cac8I	GCNNGC	2 cut(s)	379, 791
Cfr10I	RCCGGY	1 cut(s)	302
Cfr13I	GGNCC	1 cut(s)	153
ClaI	ATCGAT	1 cut(s)	852
CseI	GACGC	2 cut(s)	28, 101
Csp6I	GTAC	3 cut(s)	232, 405, 1009
CviAII	CATG	5 cut(s)	422, 448, 830, 891, 1141
CviQI	GTAC	3 cut(s)	232, 405, 1009
DdeI	CTNAG	3 cut(s)	589, 1035, 1166
DpnI	GATC	7 cut(s)	123, 252, 726, 958, 990, 1110, 1149
DpnII	GATC	7 cut(s)	121, 250, 724, 956, 988, 1108, 1147
DraIII	CACNNNGTG	1 cut(s)	130
EaeI	YGGCCR	1 cut(s)	27
EagI	CGGCCG	1 cut(s)	27
EciI	GGCGGA	1 cut(s)	50
EclXI	CGGCCG	1 cut(s)	27
Eco130I	CCWWGG	1 cut(s)	447
Eco24I	GRGCYC	1 cut(s)	1182
Eco47I	GGWCC	1 cut(s)	153
Eco52I	CGGCCG	1 cut(s)	27
EcoRII	CCWGG	1 cut(s)	475
EcoT14I	CCWWGG	1 cut(s)	447
EcoT22I	ATGCAT	1 cut(s)	892
EcoT38I	GRGCYC	1 cut(s)	1182
ErhI	CCWWGG	1 cut(s)	447
FaeI	CATG	5 cut(s)	425, 451, 833, 894, 1144
FalI	AAGNNNNNCTT	4 cut(s)	687, 719, 935, 967
FaqI	GGGAC	3 cut(s)	139, 493, 1003
FatI	CATG	5 cut(s)	421, 447, 829, 890, 1140
FauNDI	CATATG	1 cut(s)	330
FbaI	TGATCA	1 cut(s)	988
Fnu4HI	GCNGC	2 cut(s)	18, 96
FokI	GGATG	1 cut(s)	899
FriOI	GRGCYC	1 cut(s)	1182
Fsp4HI	GCNGC	2 cut(s)	18, 96
FspBI	CTAG	2 cut(s)	1187, 1228
GluI	GCNGC	2 cut(s)	18, 96
GsaI	CCCAGC	2 cut(s)	110, 244
GsuI	CTGGAG	1 cut(s)	483
HaeIII	GGCC	1 cut(s)	29
HapII	CCGG	4 cut(s)	30, 89, 303, 581
HgaI	GACGC	2 cut(s)	28, 101
Hin1I	GRCGYC	2 cut(s)	39, 93
Hin1II	CATG	5 cut(s)	425, 451, 833, 894, 1144
HindIII	AAGCTT	1 cut(s)	741
HinfI	GANTC	3 cut(s)	465, 854, 1029
HpaII	CCGG	4 cut(s)	30, 89, 303, 581
HphI	GGTGA	5 cut(s)	94, 116, 773, 921, 1210
Hpy166II	GTNNAC	2 cut(s)	43, 1218
Hpy188I	TCNGA	2 cut(s)	634, 981
Hpy188III	TCNNGA	8 cut(s)	119, 156, 199, 254, 433, 521, 648, 1004
Hpy8I	GTNNAC	2 cut(s)	43, 1218
Hpy99I	CGWCG	2 cut(s)	54, 1201
HpyAV	CCTTC	3 cut(s)	134, 271, 962
HpyCH4III	ACNGT	2 cut(s)	508, 838
HpyF10VI	GCNNNNNNNGC	3 cut(s)	26, 212, 299
HpyF3I	CTNAG	3 cut(s)	589, 1035, 1166
Hsp92I	GRCGYC	2 cut(s)	39, 93
Hsp92II	CATG	5 cut(s)	425, 451, 833, 894, 1144
KpnI	GGTACC	1 cut(s)	408
Ksp22I	TGATCA	1 cut(s)	988
Kzo9I	GATC	7 cut(s)	121, 250, 724, 956, 988, 1108, 1147
Lsp1109I	GCAGC	1 cut(s)	29
LweI	GCATC	3 cut(s)	538, 566, 877
MaeI	CTAG	2 cut(s)	1187, 1228
MaeIII	GTNAC	2 cut(s)	584, 715
MalI	GATC	7 cut(s)	123, 252, 726, 958, 990, 1110, 1149
MbiI	CCGCTC	1 cut(s)	98
MboI	GATC	7 cut(s)	121, 250, 724, 956, 988, 1108, 1147
MboII	GAAGA	4 cut(s)	257, 707, 710, 854
MflI	RGATCY	2 cut(s)	956, 1108
MhlI	GDGCHC	3 cut(s)	189, 1075, 1182
MluCI	AATT	5 cut(s)	227, 531, 690, 711, 846
MlyI	GAGTC	1 cut(s)	474
MmeI	TCCRAC	1 cut(s)	612
MnlI	CCTC	9 cut(s)	56, 65, 335, 578, 739, 862, 1054, 1122, 1173
Mph1103I	ATGCAT	1 cut(s)	892
MseI	TTAA	4 cut(s)	12, 689, 918, 941
MslI	CAYNNNNRTG	3 cut(s)	446, 546, 1102
MspA1I	CMGCKG	2 cut(s)	110, 182
MspI	CCGG	4 cut(s)	30, 89, 303, 581
MspR9I	CCNGG	3 cut(s)	89, 477, 582
MvaI	CCWGG	1 cut(s)	477
MwoI	GCNNNNNNNGC	3 cut(s)	26, 212, 299
NciI	CCSGG	2 cut(s)	89, 582
NcoI	CCATGG	1 cut(s)	447
NdeI	CATATG	1 cut(s)	330
NdeII	GATC	7 cut(s)	121, 250, 724, 956, 988, 1108, 1147
NlaIII	CATG	5 cut(s)	425, 451, 833, 894, 1144
NlaIV	GGNNCC	3 cut(s)	155, 406, 1110
NmuCI	GTSAC	2 cut(s)	584, 715
NsiI	ATGCAT	1 cut(s)	892
NspI	RCATGY	1 cut(s)	425
OliI	CACNNNNGTG	1 cut(s)	1102
PasI	CCCWGGG	1 cut(s)	476
PciI	ACATGT	1 cut(s)	421
PcsI	WCGNNNNNNNCGW	1 cut(s)	1009
PfeI	GAWTC	2 cut(s)	854, 1029
PflMI	CCANNNNNTGG	1 cut(s)	133
PfoI	TCCNGGA	1 cut(s)	87
PkrI	GCNGC	2 cut(s)	19, 97
PleI	GAGTC	1 cut(s)	473
PpsI	GAGTC	1 cut(s)	473
PscI	ACATGT	1 cut(s)	421
PshBI	ATTAAT	1 cut(s)	689
Psp6I	CCWGG	1 cut(s)	475
PspFI	CCCAGC	2 cut(s)	106, 240
PspGI	CCWGG	1 cut(s)	475
PspN4I	GGNNCC	3 cut(s)	155, 406, 1110
PspPI	GGNCC	1 cut(s)	153
PsuI	RGATCY	2 cut(s)	956, 1108
PvuII	CAGCTG	1 cut(s)	110
RsaI	GTAC	3 cut(s)	233, 406, 1010
RsaNI	GTAC	3 cut(s)	232, 405, 1009
RseI	CAYNNNNRTG	3 cut(s)	446, 546, 1102
SaqAI	TTAA	4 cut(s)	12, 689, 918, 941
SatI	GCNGC	2 cut(s)	18, 96
Sau3AI	GATC	7 cut(s)	121, 250, 724, 956, 988, 1108, 1147
Sau96I	GGNCC	1 cut(s)	153
SchI	GAGTC	1 cut(s)	474
ScrFI	CCNGG	3 cut(s)	89, 477, 582
SduI	GDGCHC	3 cut(s)	189, 1075, 1182
SfaNI	GCATC	3 cut(s)	538, 566, 877
SfcI	CTRYAG	1 cut(s)	1022
SinI	GGWCC	1 cut(s)	153
SmiMI	CAYNNNNRTG	3 cut(s)	446, 546, 1102
SmlI	CTYRAG	1 cut(s)	952
SmoI	CTYRAG	1 cut(s)	952
Sse9I	AATT	5 cut(s)	227, 531, 690, 711, 846
SsiI	CCGC	4 cut(s)	61, 96, 182, 487
SspI	AATATT	1 cut(s)	1154
SspMI	CTAG	2 cut(s)	1187, 1228
StyD4I	CCNGG	3 cut(s)	87, 475, 580
StyI	CCWWGG	1 cut(s)	447
TaaI	ACNGT	2 cut(s)	508, 838
TaqI	TCGA	5 cut(s)	120, 138, 813, 852, 1176
TasI	AATT	5 cut(s)	227, 531, 690, 711, 846
TatI	WGTACW	1 cut(s)	231
TauI	GCSGC	1 cut(s)	98
TfiI	GAWTC	2 cut(s)	854, 1029
Tru1I	TTAA	4 cut(s)	12, 689, 918, 941
Tru9I	TTAA	4 cut(s)	12, 689, 918, 941
TscAI	CASTG	3 cut(s)	289, 724, 998
TseFI	GTSAC	2 cut(s)	584, 715
TseI	GCWGC	1 cut(s)	17
Tsp45I	GTSAC	2 cut(s)	584, 715
TspGWI	ACGGA	2 cut(s)	38, 1214
TspRI	CASTG	3 cut(s)	289, 724, 998
Van91I	CCANNNNNTGG	1 cut(s)	133
VpaK11BI	GGWCC	1 cut(s)	153
VspI	ATTAAT	1 cut(s)	689
XceI	RCATGY	1 cut(s)	425
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1141
XspI	CTAG	2 cut(s)	1187, 1228
Zsp2I	ATGCAT	1 cut(s)	892

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.