Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0004184

Physical Location & Seq

Genomic Coordinates Forward (+)

27999647 .. 28006260

Gene Structure 6614 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00306970.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1161 bp

ATGATGCAGAGGCTCAAGGCAGCTGTATTGGCCAGGCGCGGCAGCCTTGACAGTTGTCGTTTCCTCAGCCATTGCAGCAGAAGAGGTCTCTGCTCTCTCCCGGACACTGCCCTCAACGATGATCTTGATAACCAAGTATTGGTTGAAGGCAAAGCTTGGTCCAGAACTGCAGTTCTCAACAGACCTTCAGTGCTCAATGCTCTCACTACTGCAATGGGGCTTAGATTGCAGAAACTGTACAGAAGTTGGGAAGATAATCCGGATGTGGGCTTTATTGCATTGAAGGGCAGTGGCAAGGCATTTTGTGCGGGTGGAGATGTAGTGTCTCTCTATCATTTGATAAACCAAGGTAGAACTGTCGTGGCTATCTTGAATGGCGTTACCATGGGTGGCGGTGGTGGTGTCTCAATCCCTGGAACATTCCGCATTGCAACTGATAAAACTGTTTTCGCTACCCCTGAAACTCTAATTGGTTTCCATCCAGATGCTGGGGCATCCTTTTACCTTTCACATCTACCGGGTCACTTAGGGGAGTTCTTGGCTCTTACAAGTGAAAAACTCAATGGACCTGAGATGCTTGCTTGTGGGCTTGCTACACACTATTTGCATAGTTCAAGGATTCCATTAATCGAAGAAGAACTTGGGAAGTTAGTCACTGATGATCCTTCTGTCATTGAATCTAGTTTAGACAAACATGGGGAACTTGTCCATCCAGATAAGTCAAGTGTGCTTCACAGGATTGAATTACTTGATGAATGTTTCAGCCATGACACAGTTGAAGAGATTATAGATGCCTTGGAAATTGAGGCAGGCAGGACAAAGGATCCATGGTGCATTTCTACTCTAAAGAGACTTAAAGAAGTCTCTCCATTGAGCTTGAAGGTTTCCCTTAGATCTATAAGAGAAGGAAGATTTCAGACCCTTGATCAGTGCTTGGTCCGTGAGTACCGAATGTCCTTACAGGGATTTTCTAAGCAGATTACCGGTGACTTCTGTGAGGGTGTTCGGGCACGACTAGTCGAAAAGGACTCAGCCCCAAAGTGGGATCCTCCTAGTGTTGAAAAAGTAACCAAAGACATGGTGGATCAATATTTTGTCCCTCTTAGTGAGTTTGAGCCTGACTTAGAACTACCAACAGAGTCGCGAGAAGCATTCATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200

Pfam Domains

Protein Families

ECH_2

Protein Analysis

386

Amino Acids

42.56

Weight (kDa)

5.62

Isoelectric Point (pI)

39.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_2	PF16113	55 - 117	1.4e-17	Enoyl-CoA hydratase/isomerase
ECH_1	PF00378	59 - 199	5.5e-15	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	119 - 365	7.3e-86	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G60510 AT3G60510 AT3G60510
fragaria_vesca	FvH4_7g10610 FvH4_7g10610 FvH4_7g10610 FvH4_7g10610 FvH4_7g10610 FvH4_7g10610 FvH4_7g10610 FvH4_7g10610
malus_domestica	MD07G1111300.v1.1
prunus_persica	Prupe.2G134700_v2.0.a1 Prupe.2G134700_v2.0.a1 Prupe.2G134700_v2.0.a1 Prupe.2G134700_v2.0.a1 Prupe.2G134700_v2.0.a1
pyrus_communis	pycom07g09610
rosa_chinensis	RchiOBHm_Chr1g0348451 RchiOBHm_Chr1g0348531
rosa_laevigata	RLG00000028641 RLG00000028649 RLG00000028657
rosa_multiflora	Rmu_sc0000091.1_g000013
rosa_roxburghii	Rroxscaffold_4G00306970
rosa_rugosa	Rorug01G0194700 Rorug01G0194800
rosa_samantha	Rh1AG212800 Rh1BG179200 Rh1CG197100 Rh1DG208900 Rh1DG209300
rosa_wichuraiana	Rw1G018010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	139, 488
AccII	CGCG	2 cut(s)	39, 1144
AccIII	TCCGGA	1 cut(s)	259
AciI	CCGC	4 cut(s)	39, 308, 393, 424
AclWI	GGATC	6 cut(s)	656, 818, 831, 1040, 1053, 1092
AcoI	YGGCCR	1 cut(s)	30
AcuI	CTGAAG	1 cut(s)	171
AfaI	GTAC	2 cut(s)	239, 947
AfiI	CCNNNNNNNGG	5 cut(s)	139, 266, 488, 1041, 1042
AgeI	ACCGGT	1 cut(s)	983
AgsI	TTSAA	9 cut(s)	146, 283, 373, 615, 677, 743, 779, 880, 1061
AhlI	ACTAGT	1 cut(s)	1015
AjnI	CCWGG	2 cut(s)	32, 412
AjuI	GAANNNNNNNTTGG	4 cut(s)	453, 485, 624, 656
AluBI	AGCT	3 cut(s)	23, 155, 876
AluI	AGCT	3 cut(s)	23, 155, 876
Alw21I	GWGCWC	1 cut(s)	195
Alw26I	GTCTC	5 cut(s)	92, 330, 409, 844, 868
AlwI	GGATC	6 cut(s)	656, 818, 831, 1040, 1053, 1092
AlwNI	CAGNNNCTG	2 cut(s)	235, 488
Aor13HI	TCCGGA	1 cut(s)	259
AoxI	GGCC	1 cut(s)	30
ApeKI	GCWGC	3 cut(s)	20, 42, 75
AseI	ATTAAT	1 cut(s)	626
AsiGI	ACCGGT	1 cut(s)	983
AspLEI	GCGC	1 cut(s)	39
AspS9I	GGNCC	3 cut(s)	159, 566, 937
AsuC2I	CCSGG	2 cut(s)	101, 519
AsuHPI	GGTGA	1 cut(s)	998
AvaII	GGWCC	3 cut(s)	159, 566, 937
BaeGI	GKGCMC	1 cut(s)	1012
BalI	TGGCCA	1 cut(s)	32
BamHI	GGATCC	2 cut(s)	823, 1045
Bbv12I	GWGCWC	1 cut(s)	195
BbvCI	CCTCAGC	1 cut(s)	65
BbvI	GCAGC	3 cut(s)	32, 54, 87
BccI	CCATC	2 cut(s)	486, 717
BciT130I	CCWGG	2 cut(s)	34, 414
BclI	TGATCA	1 cut(s)	925
BcnI	CCSGG	2 cut(s)	101, 519
BcoDI	GTCTC	5 cut(s)	92, 330, 409, 844, 868
BcuI	ACTAGT	1 cut(s)	1015
BfaI	CTAG	3 cut(s)	681, 1016, 1053
BfmI	CTRYAG	1 cut(s)	168
BglII	AGATCT	1 cut(s)	893
BisI	GCNGC	4 cut(s)	21, 40, 43, 76
BlsI	GCNGC	4 cut(s)	22, 41, 44, 77
Bme1390I	CCNGG	4 cut(s)	34, 101, 414, 519
Bme18I	GGWCC	3 cut(s)	159, 566, 937
BmgT120I	GGNCC	3 cut(s)	159, 566, 937
BmiI	GGNNCC	2 cut(s)	825, 1047
BmrFI	CCNGG	4 cut(s)	34, 101, 414, 519
BmsI	GCATC	4 cut(s)	475, 503, 564, 781
BoxI	GACNNNNGTC	1 cut(s)	54
Bpu10I	CCTNAGC	1 cut(s)	65
BpuMI	CCSGG	2 cut(s)	101, 519
BsaI	GGTCTC	1 cut(s)	92
BsaJI	CCNNGG	5 cut(s)	346, 384, 412, 795, 827
BsaWI	WCCGGW	2 cut(s)	259, 983
BsaXI	ACNNNNNCTCC	2 cut(s)	306, 336
Bsc4I	CCNNNNNNNGG	5 cut(s)	139, 266, 488, 1041, 1042
Bse118I	RCCGGY	1 cut(s)	983
Bse3DI	GCAATG	3 cut(s)	70, 219, 426
BseAI	TCCGGA	1 cut(s)	259
BseBI	CCWGG	2 cut(s)	34, 414
BseDI	CCNNGG	5 cut(s)	346, 384, 412, 795, 827
BseGI	GGATG	4 cut(s)	268, 478, 494, 709
BseLI	CCNNNNNNNGG	5 cut(s)	139, 266, 488, 1041, 1042
BseMI	GCAATG	3 cut(s)	70, 219, 426
BseMII	CTCAG	3 cut(s)	79, 561, 1044
BseSI	GKGCMC	1 cut(s)	1012
BseXI	GCAGC	3 cut(s)	32, 54, 87
BseYI	CCCAGC	1 cut(s)	488
Bsh1236I	CGCG	2 cut(s)	39, 1144
BshFI	GGCC	1 cut(s)	32
BshTI	ACCGGT	1 cut(s)	983
BsiHKAI	GWGCWC	1 cut(s)	195
BsiSI	CCGG	4 cut(s)	101, 260, 518, 984
BslFI	GGGAC	1 cut(s)	1082
BslI	CCNNNNNNNGG	5 cut(s)	139, 266, 488, 1041, 1042
BsmAI	GTCTC	5 cut(s)	92, 330, 409, 844, 868
BsmFI	GGGAC	1 cut(s)	1082
BsmI	GAATGC	1 cut(s)	1151
BsnI	GGCC	1 cut(s)	32
Bso31I	GGTCTC	1 cut(s)	92
Bsp1286I	GDGCHC	2 cut(s)	195, 1012
Bsp13I	TCCGGA	1 cut(s)	259
Bsp1407I	TGTACA	1 cut(s)	237
Bsp143I	GATC	7 cut(s)	121, 661, 823, 893, 925, 1045, 1084
Bsp19I	CCATGG	2 cut(s)	384, 827
Bsp68I	TCGCGA	1 cut(s)	1144
BspACI	CCGC	4 cut(s)	39, 308, 393, 424
BspANI	GGCC	1 cut(s)	32
BspCNI	CTCAG	3 cut(s)	78, 562, 1043
BspEI	TCCGGA	1 cut(s)	259
BspFNI	CGCG	2 cut(s)	39, 1144
BspLI	GGNNCC	2 cut(s)	825, 1047
BspMAI	CTGCAG	1 cut(s)	172
BspPI	GGATC	6 cut(s)	656, 818, 831, 1040, 1053, 1092
BspTNI	GGTCTC	1 cut(s)	92
BsrDI	GCAATG	3 cut(s)	70, 219, 426
BsrFI	RCCGGY	1 cut(s)	983
BsrGI	TGTACA	1 cut(s)	237
BssAI	RCCGGY	1 cut(s)	983
BssECI	CCNNGG	5 cut(s)	346, 384, 412, 795, 827
BssMI	GATC	7 cut(s)	121, 661, 823, 893, 925, 1045, 1084
BssT1I	CCWWGG	4 cut(s)	346, 384, 795, 827
Bst2UI	CCWGG	2 cut(s)	34, 414
Bst4CI	ACNGT	5 cut(s)	53, 237, 358, 445, 775
Bst6I	CTCTTC	2 cut(s)	76, 774
BstAPI	GCANNNNNTGC	1 cut(s)	305
BstAUI	TGTACA	1 cut(s)	237
BstC8I	GCNNGC	3 cut(s)	579, 591, 811
BstDEI	CTNAG	9 cut(s)	65, 221, 526, 570, 890, 972, 1030, 1103, 1123
BstDSI	CCRYGG	2 cut(s)	384, 827
BstF5I	GGATG	4 cut(s)	268, 478, 494, 709
BstFNI	CGCG	2 cut(s)	39, 1144
BstHHI	GCGC	1 cut(s)	39
BstKTI	GATC	7 cut(s)	124, 664, 826, 896, 928, 1048, 1087
BstMAI	GTCTC	5 cut(s)	92, 330, 409, 844, 868
BstMBI	GATC	7 cut(s)	121, 661, 823, 893, 925, 1045, 1084
BstMWI	GCNNNNNNNGC	4 cut(s)	29, 75, 226, 305
BstNI	CCWGG	2 cut(s)	34, 414
BstPAI	GACNNNNGTC	1 cut(s)	54
BstSCI	CCNGG	4 cut(s)	32, 99, 412, 517
BstSFI	CTRYAG	1 cut(s)	168
BstSLI	GKGCMC	1 cut(s)	1012
BstUI	CGCG	2 cut(s)	39, 1144
BstV1I	GCAGC	3 cut(s)	32, 54, 87
BstX2I	RGATCY	3 cut(s)	823, 893, 1045
BstXI	CCANNNNNNTGG	1 cut(s)	1078
BstYI	RGATCY	3 cut(s)	823, 893, 1045
BsuRI	GGCC	1 cut(s)	32
BtgI	CCRYGG	2 cut(s)	384, 827
BtsCI	GGATG	4 cut(s)	268, 478, 494, 709
BtsI	GCAGTG	2 cut(s)	105, 295
BtsIMutI	CAGTG	5 cut(s)	105, 195, 295, 654, 935
BtuMI	TCGCGA	1 cut(s)	1144
Cac8I	GCNNGC	3 cut(s)	579, 591, 811
CaiI	CAGNNNCTG	2 cut(s)	235, 488
CfoI	GCGC	1 cut(s)	39
Cfr10I	RCCGGY	1 cut(s)	983
Cfr13I	GGNCC	3 cut(s)	159, 566, 937
Csp6I	GTAC	2 cut(s)	238, 946
CspAI	ACCGGT	1 cut(s)	983
CviAII	CATG	5 cut(s)	385, 695, 767, 828, 1078
CviQI	GTAC	2 cut(s)	238, 946
DdeI	CTNAG	9 cut(s)	65, 221, 526, 570, 890, 972, 1030, 1103, 1123
DpnI	GATC	7 cut(s)	123, 663, 825, 895, 927, 1047, 1086
DpnII	GATC	7 cut(s)	121, 661, 823, 893, 925, 1045, 1084
EaeI	YGGCCR	1 cut(s)	30
Eam1104I	CTCTTC	2 cut(s)	76, 774
EarI	CTCTTC	2 cut(s)	76, 774
Eco130I	CCWWGG	4 cut(s)	346, 384, 795, 827
Eco31I	GGTCTC	1 cut(s)	92
Eco47I	GGWCC	3 cut(s)	159, 566, 937
Eco57I	CTGAAG	1 cut(s)	171
EcoRII	CCWGG	2 cut(s)	32, 412
EcoT14I	CCWWGG	4 cut(s)	346, 384, 795, 827
ErhI	CCWWGG	4 cut(s)	346, 384, 795, 827
FaeI	CATG	5 cut(s)	388, 698, 770, 831, 1081
FaiI	YATR	8 cut(s)	386, 609, 696, 768, 788, 829, 899, 1079
FalI	AAGNNNNNCTT	2 cut(s)	624, 656
FaqI	GGGAC	1 cut(s)	1082
FatI	CATG	5 cut(s)	384, 694, 766, 827, 1077
FauI	CCCGC	1 cut(s)	301
FbaI	TGATCA	1 cut(s)	925
Fnu4HI	GCNGC	4 cut(s)	21, 40, 43, 76
FokI	GGATG	4 cut(s)	275, 465, 481, 696
Fsp4HI	GCNGC	4 cut(s)	21, 40, 43, 76
FspBI	CTAG	3 cut(s)	681, 1016, 1053
GlaI	GCGC	1 cut(s)	38
GluI	GCNGC	4 cut(s)	21, 40, 43, 76
GsaI	CCCAGC	1 cut(s)	492
HaeIII	GGCC	1 cut(s)	32
HapII	CCGG	4 cut(s)	101, 260, 518, 984
HhaI	GCGC	1 cut(s)	39
Hin1II	CATG	5 cut(s)	388, 698, 770, 831, 1081
Hin6I	GCGC	1 cut(s)	37
HinP1I	GCGC	1 cut(s)	37
HindIII	AAGCTT	1 cut(s)	153
HinfI	GANTC	4 cut(s)	619, 677, 1028, 1139
HpaII	CCGG	4 cut(s)	101, 260, 518, 984
HphI	GGTGA	1 cut(s)	998
Hpy188I	TCNGA	2 cut(s)	918, 1160
Hpy188III	TCNNGA	7 cut(s)	125, 162, 260, 370, 482, 713, 1143
HpyAV	CCTTC	6 cut(s)	140, 195, 277, 675, 874, 899
HpyCH4III	ACNGT	5 cut(s)	53, 237, 358, 445, 775
HpyCH4V	TGCA	9 cut(s)	7, 75, 170, 212, 229, 278, 431, 607, 834
HpyF10VI	GCNNNNNNNGC	4 cut(s)	29, 75, 226, 305
HpyF3I	CTNAG	9 cut(s)	65, 221, 526, 570, 890, 972, 1030, 1103, 1123
Hsp92II	CATG	5 cut(s)	388, 698, 770, 831, 1081
HspAI	GCGC	1 cut(s)	37
Kpn2I	TCCGGA	1 cut(s)	259
Ksp22I	TGATCA	1 cut(s)	925
Kzo9I	GATC	7 cut(s)	121, 661, 823, 893, 925, 1045, 1084
Lsp1109I	GCAGC	3 cut(s)	32, 54, 87
LweI	GCATC	4 cut(s)	475, 503, 564, 781
MaeI	CTAG	3 cut(s)	681, 1016, 1053
MaeIII	GTNAC	5 cut(s)	379, 521, 652, 986, 1066
MalI	GATC	7 cut(s)	123, 663, 825, 895, 927, 1047, 1086
MboI	GATC	7 cut(s)	121, 661, 823, 893, 925, 1045, 1084
MboII	GAAGA	6 cut(s)	93, 263, 644, 647, 791, 921
MflI	RGATCY	3 cut(s)	823, 893, 1045
MhlI	GDGCHC	2 cut(s)	195, 1012
MlsI	TGGCCA	1 cut(s)	32
MluCI	AATT	3 cut(s)	468, 743, 801
MluNI	TGGCCA	1 cut(s)	32
MlyI	GAGTC	2 cut(s)	1022, 1148
MnlI	CCTC	8 cut(s)	3, 74, 77, 122, 799, 991, 1059, 1110
Mox20I	TGGCCA	1 cut(s)	32
MroI	TCCGGA	1 cut(s)	259
MscI	TGGCCA	1 cut(s)	32
MseI	TTAA	2 cut(s)	626, 855
MslI	CAYNNNNRTG	1 cut(s)	483
Msp20I	TGGCCA	1 cut(s)	32
MspA1I	CMGCKG	1 cut(s)	23
MspI	CCGG	4 cut(s)	101, 260, 518, 984
MspR9I	CCNGG	4 cut(s)	34, 101, 414, 519
Mva1269I	GAATGC	1 cut(s)	1151
MvaI	CCWGG	2 cut(s)	34, 414
MvnI	CGCG	2 cut(s)	39, 1144
MwoI	GCNNNNNNNGC	4 cut(s)	29, 75, 226, 305
NciI	CCSGG	2 cut(s)	101, 519
NcoI	CCATGG	2 cut(s)	384, 827
NdeII	GATC	7 cut(s)	121, 661, 823, 893, 925, 1045, 1084
NlaIII	CATG	5 cut(s)	388, 698, 770, 831, 1081
NlaIV	GGNNCC	2 cut(s)	825, 1047
NmuCI	GTSAC	3 cut(s)	521, 652, 986
NruI	TCGCGA	1 cut(s)	1144
PctI	GAATGC	1 cut(s)	1151
PfeI	GAWTC	2 cut(s)	619, 677
PflMI	CCANNNNNTGG	2 cut(s)	139, 488
PfoI	TCCNGGA	1 cut(s)	99
PinAI	ACCGGT	1 cut(s)	983
PkrI	GCNGC	4 cut(s)	22, 41, 44, 77
PleI	GAGTC	2 cut(s)	1022, 1147
PpsI	GAGTC	2 cut(s)	1022, 1147
PshAI	GACNNNNGTC	1 cut(s)	54
PshBI	ATTAAT	1 cut(s)	626
Psp6I	CCWGG	2 cut(s)	32, 412
PspFI	CCCAGC	1 cut(s)	488
PspGI	CCWGG	2 cut(s)	32, 412
PspN4I	GGNNCC	2 cut(s)	825, 1047
PspPI	GGNCC	3 cut(s)	159, 566, 937
PstI	CTGCAG	1 cut(s)	172
PstNI	CAGNNNCTG	2 cut(s)	235, 488
PsuI	RGATCY	3 cut(s)	823, 893, 1045
PvuII	CAGCTG	1 cut(s)	23
RruI	TCGCGA	1 cut(s)	1144
RsaI	GTAC	2 cut(s)	239, 947
RsaNI	GTAC	2 cut(s)	238, 946
RseI	CAYNNNNRTG	1 cut(s)	483
SaqAI	TTAA	2 cut(s)	626, 855
SatI	GCNGC	4 cut(s)	21, 40, 43, 76
Sau3AI	GATC	7 cut(s)	121, 661, 823, 893, 925, 1045, 1084
Sau96I	GGNCC	3 cut(s)	159, 566, 937
SchI	GAGTC	2 cut(s)	1022, 1148
ScrFI	CCNGG	4 cut(s)	34, 101, 414, 519
SduI	GDGCHC	2 cut(s)	195, 1012
SetI	ASST	9 cut(s)	25, 88, 157, 187, 352, 507, 571, 878, 885
SfaNI	GCATC	4 cut(s)	475, 503, 564, 781
SfcI	CTRYAG	1 cut(s)	168
SinI	GGWCC	3 cut(s)	159, 566, 937
SmiMI	CAYNNNNRTG	1 cut(s)	483
SmlI	CTYRAG	1 cut(s)	14
SmoI	CTYRAG	1 cut(s)	14
SpeI	ACTAGT	1 cut(s)	1015
Sse9I	AATT	3 cut(s)	468, 743, 801
SsiI	CCGC	4 cut(s)	39, 308, 393, 424
SspI	AATATT	1 cut(s)	1091
SspMI	CTAG	3 cut(s)	681, 1016, 1053
StyD4I	CCNGG	4 cut(s)	32, 99, 412, 517
StyI	CCWWGG	4 cut(s)	346, 384, 795, 827
TaaI	ACNGT	5 cut(s)	53, 237, 358, 445, 775
TaqI	TCGA	2 cut(s)	630, 1020
TasI	AATT	3 cut(s)	468, 743, 801
TatI	WGTACW	1 cut(s)	237
TauI	GCSGC	1 cut(s)	42
TfiI	GAWTC	2 cut(s)	619, 677
Tru1I	TTAA	2 cut(s)	626, 855
Tru9I	TTAA	2 cut(s)	626, 855
TscAI	CASTG	5 cut(s)	112, 195, 295, 661, 935
TseFI	GTSAC	3 cut(s)	521, 652, 986
TseI	GCWGC	3 cut(s)	20, 42, 75
Tsp45I	GTSAC	3 cut(s)	521, 652, 986
TspDTI	ATGAA	2 cut(s)	768, 1144
TspGWI	ACGGA	1 cut(s)	929
TspRI	CASTG	5 cut(s)	112, 195, 295, 661, 935
Van91I	CCANNNNNTGG	2 cut(s)	139, 488
VpaK11BI	GGWCC	3 cut(s)	159, 566, 937
VspI	ATTAAT	1 cut(s)	626
XcmI	CCANNNNNNNNNTGG	2 cut(s)	485, 1078
XspI	CTAG	3 cut(s)	681, 1016, 1053

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.