Rorug01G0194800

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0004184
Physical Location & Seq
Forward (+)
27285057 .. 27287070
2014 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0194800.1

Sequence Viewer

Length: 1173 bp
ATGACACTTGTAAAACCTACACCACATAGTAGTGTCTTTCCTGTCCTGGATCGTCTGGTTGTATTTGGAGGAAGTAGGCTGAAGGAGATTTTTCATGGTGAGCTGCCAATGGGATCTCTTAAGAAACTACGTGAGATGGATTTACTATGTCTACCTTCATTGACCTATATTTGGAAGACCAAAAGTCATTCTGAATGCTTGAGAAATATTCTAGGATTCATTGAAGTCCTAAAAATAAAAAACTGCCCGTCGTTGGAAGAGATCGTTGCCTTGGAAGGATCAAGACATCAGGAGGAAGAAGCAACCAAAGAGATCAACTTCCAAAGATTGACAGAATTGACTCTTGCAGATCTACCAAGTTTCAGTGGGATAAGCAAAAATATCTATAAAAAGGAGTTGGAAAGGCTACCGGATGTTATCAGTCTCTATCCAAATGTTTTGACAGTGGAACATTCTAATGTGTCCATACAACATCTGTTTGACACCAAGGTTCAATTTCCAGTTTTACGAAGACTAAAAATTGAAGGCATGGACAACTTAAAAGAGATATGGAACAGCGAACTTTCACCTGATTCCTTCTGTGAACTAAAGTTTCTAATAGTGAACAGATGTAACAAATTATTGCATTTGGTGCCAACACATATGCAAAATAGATTGCAGAAAATGGAGTTCATATGTGCATCACACTGTTCTTCACTGGAAGAGATCTTTGATTTCAGGATGCAAAGGAACAAGATCATGGATTTCAAACAATCTTGTCAAGCCTTTCAGAATCTAACAGATATATCACTCTGGGAATGTAACGGTGTTAGAATTTTGCTCTCTCCTTCAATTTCCGGGGGTCTTGTGAAACTCAAAGAACTAAGGATACAGCGGTGCCAGAAGATTGAAGAAATAGTAGCAGCAGCAGCAGAGGGTGAAGAAACAGAGGATAGCAACATGTTCCCTCAGCTGAGTAAATTGGTACTTGAGGATCTACCAAATCTTCGAAGCTTTTCAGAAGGCAAATATAATTTTGAATGGCAACTGATGAAATATATAGATATTGGAAAATGCTATATGAAGCATTTTTGTTTTGGATCCCTCAGCATTCCAAGGGAAGTGATTATATATGTTAGTGATGCTGGTGAGAATCTTTTGCAGGAGCTCAATAACAGTAGAAAAGAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200
Pfam Domains
Protein Families
ECH_2

Protein Analysis

390

Amino Acids

45.0

Weight (kDa)

6.25

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 70 - 125 7.9e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 171 - 247 1.9e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 250 - 352 7.2e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 631, 876
AccI GTMKAC 1 cut(s) 151
AciI CCGC 1 cut(s) 874
AclWI GGATC 6 cut(s) 57, 121, 286, 981, 1074, 1087
AcsI RAATTY 1 cut(s) 813
AcuI CTGAAG 1 cut(s) 101
AfaI GTAC 1 cut(s) 966
AfiI CCNNNNNNNGG 2 cut(s) 171, 253
AflII CTTAAG 1 cut(s) 119
AflIII ACRYGT 1 cut(s) 939
AgsI TTSAA 7 cut(s) 224, 494, 524, 748, 831, 890, 1019
AhdI GACNNNNNGTC 1 cut(s) 183
AjnI CCWGG 1 cut(s) 45
AluBI AGCT 4 cut(s) 103, 952, 993, 1147
AluI AGCT 4 cut(s) 103, 952, 993, 1147
Alw21I GWGCWC 1 cut(s) 1149
Alw26I GTCTC 1 cut(s) 428
AlwI GGATC 6 cut(s) 57, 121, 286, 981, 1074, 1087
ApeKI GCWGC 4 cut(s) 103, 902, 905, 908
ApoI RAATTY 1 cut(s) 813
Asp700I GAANNNNTTC 1 cut(s) 994
AsuC2I CCSGG 1 cut(s) 838
AsuHPI GGTGA 4 cut(s) 110, 558, 929, 1139
AsuII TTCGAA 1 cut(s) 988
BaeI ACNNNNGTAYC 2 cut(s) 860, 893
BamHI GGATCC 1 cut(s) 1079
BanI GGYRCC 2 cut(s) 631, 876
BanII GRGCYC 1 cut(s) 1149
BbsI GAAGAC 2 cut(s) 182, 517
Bbv12I GWGCWC 1 cut(s) 1149
BbvCI CCTCAGC 2 cut(s) 948, 1085
BbvI GCAGC 4 cut(s) 90, 914, 917, 920
BccI CCATC 1 cut(s) 130
BciT130I CCWGG 1 cut(s) 47
BciVI GTATCC 1 cut(s) 861
BcnI CCSGG 1 cut(s) 838
BcoDI GTCTC 1 cut(s) 428
BfaI CTAG 1 cut(s) 212
BfrI CTTAAG 1 cut(s) 119
BfuI GTATCC 1 cut(s) 861
BglII AGATCT 2 cut(s) 349, 705
BisI GCNGC 4 cut(s) 104, 903, 906, 909
BlsI GCNGC 4 cut(s) 105, 904, 907, 910
Bme1390I CCNGG 2 cut(s) 47, 838
BmeRI GACNNNNNGTC 1 cut(s) 183
BmiI GGNNCC 3 cut(s) 633, 878, 1081
BmrFI CCNGG 2 cut(s) 47, 838
BmsI GCATC 3 cut(s) 689, 711, 1111
BpiI GAAGAC 2 cut(s) 182, 517
Bpu10I CCTNAGC 2 cut(s) 948, 1085
Bpu14I TTCGAA 1 cut(s) 988
BpuEI CTTGAG 2 cut(s) 220, 989
BpuMI CCSGG 1 cut(s) 838
BsaAI YACGTR 1 cut(s) 131
BsaJI CCNNGG 4 cut(s) 270, 486, 837, 1094
BsaWI WCCGGW 1 cut(s) 409
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 253
Bse1I ACTGG 2 cut(s) 500, 702
BseBI CCWGG 1 cut(s) 47
BseDI CCNNGG 4 cut(s) 270, 486, 837, 1094
BseGI GGATG 2 cut(s) 418, 726
BseLI CCNNNNNNNGG 2 cut(s) 171, 253
BseMII CTCAG 3 cut(s) 944, 962, 1099
BseNI ACTGG 2 cut(s) 500, 702
BseXI GCAGC 4 cut(s) 90, 914, 917, 920
BshNI GGYRCC 2 cut(s) 631, 876
BsiHKAI GWGCWC 1 cut(s) 1149
BsiSI CCGG 2 cut(s) 410, 837
BslI CCNNNNNNNGG 2 cut(s) 171, 253
BsmAI GTCTC 1 cut(s) 428
BsmI GAATGC 2 cut(s) 200, 1089
Bsp119I TTCGAA 1 cut(s) 988
Bsp1286I GDGCHC 1 cut(s) 1149
BspACI CCGC 1 cut(s) 874
BspCNI CTCAG 3 cut(s) 945, 961, 1098
BspLI GGNNCC 3 cut(s) 633, 878, 1081
BspPI GGATC 6 cut(s) 57, 121, 286, 981, 1074, 1087
BspT104I TTCGAA 1 cut(s) 988
BspT107I GGYRCC 2 cut(s) 631, 876
BspTI CTTAAG 1 cut(s) 119
BsrI ACTGG 2 cut(s) 500, 702
BssECI CCNNGG 4 cut(s) 270, 486, 837, 1094
BssT1I CCWWGG 3 cut(s) 270, 486, 1094
Bst2UI CCWGG 1 cut(s) 47
Bst4CI ACNGT 4 cut(s) 445, 689, 806, 1157
Bst6I CTCTTC 2 cut(s) 252, 696
BstAFI CTTAAG 1 cut(s) 119
BstAPI GCANNNNNTGC 1 cut(s) 631
BstBAI YACGTR 1 cut(s) 131
BstBI TTCGAA 1 cut(s) 988
BstDEI CTNAG 4 cut(s) 863, 948, 953, 1085
BstF5I GGATG 2 cut(s) 418, 726
BstMAI GTCTC 1 cut(s) 428
BstMWI GCNNNNNNNGC 2 cut(s) 631, 908
BstNI CCWGG 1 cut(s) 47
BstNSI RCATGY 1 cut(s) 943
BstSCI CCNGG 2 cut(s) 45, 836
BstV1I GCAGC 4 cut(s) 90, 914, 917, 920
BstV2I GAAGAC 2 cut(s) 182, 517
BstX2I RGATCY 5 cut(s) 113, 349, 705, 973, 1079
BstYI RGATCY 5 cut(s) 113, 349, 705, 973, 1079
BsuI GTATCC 1 cut(s) 861
BtsCI GGATG 2 cut(s) 418, 726
BtsIMutI CAGTG 4 cut(s) 370, 450, 685, 695
Csp6I GTAC 1 cut(s) 965
CviAII CATG 4 cut(s) 95, 529, 739, 940
CviJI RGCY 7 cut(s) 79, 103, 406, 764, 952, 993, 1147
CviKI_1 RGCY 7 cut(s) 79, 103, 406, 764, 952, 993, 1147
CviQI GTAC 1 cut(s) 965
DdeI CTNAG 4 cut(s) 863, 948, 953, 1085
DriI GACNNNNNGTC 1 cut(s) 183
Eam1104I CTCTTC 2 cut(s) 252, 696
Eam1105I GACNNNNNGTC 1 cut(s) 183
EarI CTCTTC 2 cut(s) 252, 696
Ecl136II GAGCTC 1 cut(s) 1147
Eco130I CCWWGG 3 cut(s) 270, 486, 1094
Eco24I GRGCYC 1 cut(s) 1149
Eco53kI GAGCTC 1 cut(s) 1147
Eco57I CTGAAG 1 cut(s) 101
EcoICRI GAGCTC 1 cut(s) 1147
EcoRII CCWGG 1 cut(s) 45
EcoT14I CCWWGG 3 cut(s) 270, 486, 1094
EcoT38I GRGCYC 1 cut(s) 1149
ErhI CCWWGG 3 cut(s) 270, 486, 1094
FaeI CATG 4 cut(s) 98, 532, 742, 943
FatI CATG 4 cut(s) 94, 528, 738, 939
FauNDI CATATG 2 cut(s) 642, 674
FblI GTMKAC 1 cut(s) 151
Fnu4HI GCNGC 4 cut(s) 104, 903, 906, 909
FokI GGATG 2 cut(s) 425, 733
FriOI GRGCYC 1 cut(s) 1149
Fsp4HI GCNGC 4 cut(s) 104, 903, 906, 909
FspBI CTAG 1 cut(s) 212
GluI GCNGC 4 cut(s) 104, 903, 906, 909
HapII CCGG 2 cut(s) 410, 837
Hin1II CATG 4 cut(s) 98, 532, 742, 943
HindIII AAGCTT 1 cut(s) 991
HinfI GANTC 5 cut(s) 216, 340, 572, 772, 1132
HpaII CCGG 2 cut(s) 410, 837
HphI GGTGA 4 cut(s) 110, 558, 929, 1139
Hpy166II GTNNAC 3 cut(s) 152, 584, 604
Hpy188I TCNGA 3 cut(s) 193, 771, 1000
Hpy188III TCNNGA 3 cut(s) 282, 290, 718
Hpy8I GTNNAC 3 cut(s) 152, 584, 604
Hpy99I CGWCG 1 cut(s) 253
HpyAV CCTTC 7 cut(s) 76, 165, 269, 518, 586, 837, 995
HpyCH4III ACNGT 4 cut(s) 445, 689, 806, 1157
HpyCH4IV ACGT 1 cut(s) 130
HpyCH4V TGCA 7 cut(s) 347, 625, 646, 658, 680, 724, 1141
HpyF10VI GCNNNNNNNGC 2 cut(s) 631, 908
HpyF3I CTNAG 4 cut(s) 863, 948, 953, 1085
HpySE526I ACGT 1 cut(s) 130
Hsp92II CATG 4 cut(s) 98, 532, 742, 943
LmnI GCTCC 1 cut(s) 1144
Lsp1109I GCAGC 4 cut(s) 90, 914, 917, 920
LweI GCATC 3 cut(s) 689, 711, 1111
MaeI CTAG 1 cut(s) 212
MaeII ACGT 1 cut(s) 130
MaeIII GTNAC 2 cut(s) 611, 800
MflI RGATCY 5 cut(s) 113, 349, 705, 973, 1079
MhlI GDGCHC 1 cut(s) 1149
MluCI AATT 8 cut(s) 335, 494, 519, 617, 813, 831, 959, 1012
MlyI GAGTC 1 cut(s) 334
MmeI TCCRAC 2 cut(s) 234, 378
MnlI CCTC 7 cut(s) 62, 286, 907, 922, 957, 964, 1094
MroXI GAANNNNTTC 1 cut(s) 994
MseI TTAA 2 cut(s) 120, 539
MslI CAYNNNNRTG 2 cut(s) 30, 456
MspA1I CMGCKG 2 cut(s) 874, 952
MspCI CTTAAG 1 cut(s) 119
MspI CCGG 2 cut(s) 410, 837
MspR9I CCNGG 2 cut(s) 47, 838
Mva1269I GAATGC 2 cut(s) 200, 1089
MvaI CCWGG 1 cut(s) 47
MwoI GCNNNNNNNGC 2 cut(s) 631, 908
NciI CCSGG 1 cut(s) 838
NdeI CATATG 2 cut(s) 642, 674
NlaIII CATG 4 cut(s) 98, 532, 742, 943
NlaIV GGNNCC 3 cut(s) 633, 878, 1081
NspI RCATGY 1 cut(s) 943
NspV TTCGAA 1 cut(s) 988
PciI ACATGT 1 cut(s) 939
PctI GAATGC 2 cut(s) 200, 1089
PdmI GAANNNNTTC 1 cut(s) 994
PfeI GAWTC 4 cut(s) 216, 572, 772, 1132
PfoI TCCNGGA 1 cut(s) 45
PkrI GCNGC 4 cut(s) 105, 904, 907, 910
PleI GAGTC 1 cut(s) 334
PpsI GAGTC 1 cut(s) 334
Ppu21I YACGTR 1 cut(s) 131
PscI ACATGT 1 cut(s) 939
Psp124BI GAGCTC 1 cut(s) 1149
Psp6I CCWGG 1 cut(s) 45
PspGI CCWGG 1 cut(s) 45
PspN4I GGNNCC 3 cut(s) 633, 878, 1081
PsrI GAACNNNNNNTAC 2 cut(s) 852, 884
PsuI RGATCY 5 cut(s) 113, 349, 705, 973, 1079
PvuII CAGCTG 1 cut(s) 952
RsaI GTAC 1 cut(s) 966
RsaNI GTAC 1 cut(s) 965
RseI CAYNNNNRTG 2 cut(s) 30, 456
SacI GAGCTC 1 cut(s) 1149
SaqAI TTAA 2 cut(s) 120, 539
SatI GCNGC 4 cut(s) 104, 903, 906, 909
SchI GAGTC 1 cut(s) 334
ScrFI CCNGG 2 cut(s) 47, 838
SduI GDGCHC 1 cut(s) 1149
SfaNI GCATC 3 cut(s) 689, 711, 1111
SfuI TTCGAA 1 cut(s) 988
SmiMI CAYNNNNRTG 2 cut(s) 30, 456
SmlI CTYRAG 3 cut(s) 119, 199, 968
SmoI CTYRAG 3 cut(s) 119, 199, 968
Sse9I AATT 8 cut(s) 335, 494, 519, 617, 813, 831, 959, 1012
SsiI CCGC 1 cut(s) 874
SspI AATATT 1 cut(s) 208
SspMI CTAG 1 cut(s) 212
SstI GAGCTC 1 cut(s) 1149
StyD4I CCNGG 2 cut(s) 45, 836
StyI CCWWGG 3 cut(s) 270, 486, 1094
TaaI ACNGT 4 cut(s) 445, 689, 806, 1157
TaiI ACGT 1 cut(s) 133
TaqI TCGA 1 cut(s) 988
TasI AATT 8 cut(s) 335, 494, 519, 617, 813, 831, 959, 1012
TfiI GAWTC 4 cut(s) 216, 572, 772, 1132
Tru1I TTAA 2 cut(s) 120, 539
Tru9I TTAA 2 cut(s) 120, 539
TscAI CASTG 4 cut(s) 370, 450, 692, 702
TseI GCWGC 4 cut(s) 103, 902, 905, 908
TspDTI ATGAA 6 cut(s) 83, 147, 208, 661, 1046, 1076
TspRI CASTG 4 cut(s) 370, 450, 692, 702
Vha464I CTTAAG 1 cut(s) 119
XapI RAATTY 1 cut(s) 813
XceI RCATGY 1 cut(s) 943
XmiI GTMKAC 1 cut(s) 151
XmnI GAANNNNTTC 1 cut(s) 994
XspI CTAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.