Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0006252

Physical Location & Seq

Genomic Coordinates Reverse (-)

18847971 .. 18857554

Gene Structure 9584 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1131500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2484 bp

ATGTCAAAATCTTCATCCGTTAGCTCACTTCCACTCATCTTGGACATAGACGACTTTAAGGGTGAGTTTTCATTTGATGCATTGTTTGGGAACCTGGTAAATGAGCTGCTCCCATCTTTTCAAGAAGAAGAAACAGACTCATCAGAAGGTCATAGCAACGTCAGTGGGAACGATTCTTTACAAAATGGACATATGCGAGTACCATCAGGTGCAACAAAATTTGCACAAGGACTGTCAGACCCGTTATTTCCGGAAGTAGATAAGACCTTATCTCTGTTCAAGGATTCTTGTAAAGAGTTGGTTGATCTCCAGAAACAGATTGATGGAAGACTCTACAATCTTAAGAAGGAGGTTTCTGTACAAGATTCTAAGCACCGCAAGACACTTGTTGAGCTTGAAAAAGGAGTAGATGGCTTGTTTGGTAGCTTTGCCAGGCTGGATTCTCGTATATCAAGTGTTGGCCAGACCGCTGCAAAAATAGGAGACCATCTGCAGAGTGCAGATGCTCAGAGGGAAACTGCTAGTCAAACAATAGAGCTCATAAAGTACTTGATGGAGTTCAATAGCAGCCCTGGCGATCTGATGGAACTTTCACCCTTATTTTCTGATGATAGCCGTGTTGCAGAGGCTACTAAAATTGCACAAAAATTGCGGGCATTTGCTGAGGAAGATATTGGAAGACAAGGGATATCTGTACCATCAGTTATGGGCAATGCAACAGCTAGCAGAGGGTTAGAAGTTGCAGTTGCTAATCTTCAGGACTACTGCAATGAATTGGAGAACAGATTGTTATCTCGTTTTGATGCTGCATCACAGAGAAGAGAATTGTCAACCATGGCAGAATGTGCTAAAATATTATCTCAGTTTAACAGGGGCACTAGTGCTATGCAACATTATGTGGCAACACGTCCAATGTTTATTGACGTGGAAGTCATGAATGCAGATACCAGATTGGTTCTTGGTGATGAGGGTTCACAGGCTAGTCCTAGCAATGTTGCTCGTGGACTTTTGTCCTTGTATAAAGAAATCACAGATAATGTACGTAAGGAAGCTGCAACGGTTATGGCTGTCTTCCCTTCTCCAAATGAAGTTATGTCAATCTTAGTTCAGCGAGTTTTGGAGCAGAGAGTTACTGCTCTACTGGACAAATTATTAGTGAAGCCATCTTTGGTGAATCTACCTCCTATGGAAGAAGGCGGACTTCTATTATATATTAGAATGCTAGCAGTGGCATATGAGAAGACACAAGAACTGGCTAGGGACCTACGAGTTGTGGGATGTGGTGACTTGGATATCGAGGGCCTCACAGAGTCTCTGTTTTCTTCTCACAAGGATGGATATCCTGAACATGAGCAGGCCTCTCTTAAACAACTATATCTAGCTAAGATGGCAGAACTACGTGCTGAAAGCCAACAGATATCTGAATCAAGTGGAACAATCGGGCGCTCAAAAGGTGCTGCAGTTGCATCTTCCCGTCAGCAAATATCCGTCACTGTTGTGACAGAGTTTGTCCGCTGGAATGAAGAAGCAATTGCCAGATGCACTTTGTTTTCATCTCAACCTGCAACACTTGCAGCAAATGTAAAAGCGGTGTTCACTTCCCTATTAGACCAAGTCAGTCAATATATAACGGAAGGTCTTGAGCAAGCAAGAGATGGCCTGACTGAAGCTGCAGCTTTGAGGGAAAGATTTGTACTTGGGGCTAGTGTTAGAAGGGTGGCTGCTGCTGCTGCTTCTGCTGCAGAAGCTGCTGCCGCTGCTGGTGAAAGCAGTTTCAGATCTTTCATGGTTGCTGTACAACGTTGTGGTAGTAGTGTGGCTATAGTTCAGCAGTATTTCTCAAATTCTATATCTCGGCTTCTACTCCCTGTCGATGGTGCACATGCTGCTTCTTGTGAAGAAATGGCAACAGCTATGTCCAGTGCAGAGGGTGCTGCATATAAAGGGCTTCAACAGTGCATTGAAACTGTGATGTCAGAGGTGGAGCGGTTACTTTCTGCTGAACAGAAGGCAACAGATTATAGATCACCTGAAGATGGATTTGCTCCTGATCATCGGCCAACAAATGCCTGTACAAGAGTTGTAGCATATCTTTCCCGTGTACTAGAATCAGCATTCACGGCACTTGAAGGTCTTAACAAACAAGCGTTTCTAACTGAATTAGGTAACCGCTTGCATAAGGGGCTGCTAAATCATTGGCAAAAGTTCACTTTTAATCCGAGCGGGGGACTGCGGCTGAAGCGTGACATAACTGAGTATGGAGAATTTGTGCGTAGTTTCAATGCTCCTTCCGTTGATGAAAATTTTGAATTGCTGGGGATAATGGCCAATGTCTTCATTGTTGCTCCTGAAAGTCTCTCAACTCTCTTTGAGGGTACACCTAGTATACGGAAAGATGCACAAAGGTTTATTGAGCTTCGAGAGGACTACAAGAGCGCAAAGCTTGCTGCCAGACTTAGTTCCTTGTGGACAAGTTCTAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000145 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005938 GO:0006810 GO:0006887 GO:0006892 GO:0006893 GO:0008150 GO:0009987 GO:0016192 GO:0031982 GO:0032940 GO:0032991 GO:0043226 GO:0043227 GO:0043230 GO:0044421 GO:0044424 GO:0044444 GO:0044448 GO:0044464 GO:0046903 GO:0048193 GO:0051179 GO:0051234 GO:0070062 GO:0071944 GO:0098876 GO:0099023 GO:0099568 GO:1903561

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sec10

Protein Analysis

828

Amino Acids

90.34

Weight (kDa)

5.18

Isoelectric Point (pI)

49.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sec10_N	PF20667	88 - 203	8.2e-31	Exocyst complex component Sec10, N-terminal
Sec10_HB	PF07393	210 - 817	2.7e-148	Exocyst complex component Sec10-like, alpha-helical bundle

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006252)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12370 AT5G12370 AT5G12370
fragaria_vesca	FvH4_7g15330 FvH4_7g15330 FvH4_7g15330 FvH4_7g15330
malus_domestica	MD01G1071200.v1.1 MD07G1131500.v1.1
prunus_persica	Prupe.2G177200_v2.0.a1
pyrus_communis	pycom01g10010 pycom07g12840
rosa_chinensis	RchiOBHm_Chr1g0357661 RchiOBHm_Chr1g0375651 RchiOBHm_Chr1g0375661
rosa_laevigata	RLG00000028010
rosa_multiflora	Rmu_sc0001644.1_g000021 Rmu_sc0004683.1_g000003
rosa_roxburghii	Rroxscaffold_4G00282690 Rroxscaffold_4G00298660
rosa_rugosa	Rorug01G0257500
rosa_samantha	Rh1AG271500 Rh1AG402900 Rh1BG238400 Rh1CG255300 Rh1DG266700
rosa_wichuraiana	Rw1G024200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	929
Acc36I	ACCTGC	1 cut(s)	1570
AccBSI	CCGCTC	2 cut(s)	1987, 2223
AccI	GTMKAC	1 cut(s)	2386
AccIII	TCCGGA	1 cut(s)	250
AclI	AACGTT	1 cut(s)	1801
AcoI	YGGCCR	3 cut(s)	460, 2057, 2325
AcsI	RAATTY	4 cut(s)	218, 1843, 2264, 2302
AcuI	CTGAAG	4 cut(s)	740, 1686, 2052, 2258
AfiI	CCNNNNNNNGG	3 cut(s)	1874, 2036, 2225
AflII	CTTAAG	1 cut(s)	341
AflIII	ACRYGT	1 cut(s)	905
AgsI	TTSAA	9 cut(s)	122, 280, 398, 562, 1952, 1964, 2129, 2281, 2309
AhlI	ACTAGT	1 cut(s)	878
AjiI	CACGTC	2 cut(s)	908, 925
AjnI	CCWGG	3 cut(s)	93, 431, 571
AjuI	GAANNNNNNNTTGG	2 cut(s)	1151, 1183
AleI	CACNNNNGTG	1 cut(s)	1496
Alw21I	GWGCWC	2 cut(s)	540, 1882
Alw26I	GTCTC	3 cut(s)	477, 1317, 2360
Alw44I	GTGCAC	1 cut(s)	1878
AlwNI	CAGNNNCTG	1 cut(s)	1748
Aor13HI	TCCGGA	1 cut(s)	250
AoxI	GGCC	6 cut(s)	460, 1300, 1356, 1657, 2057, 2325
ApaLI	GTGCAC	1 cut(s)	1878
ApoI	RAATTY	4 cut(s)	218, 1843, 2264, 2302
AspLEI	GCGC	2 cut(s)	1446, 2438
AspS9I	GGNCC	2 cut(s)	1261, 1300
AsuHPI	GGTGA	7 cut(s)	74, 585, 974, 1183, 1295, 1775, 2019
AsuNHI	GCTAGC	2 cut(s)	722, 1222
AvaII	GGWCC	1 cut(s)	1261
BaeGI	GKGCMC	2 cut(s)	878, 1882
BalI	TGGCCA	2 cut(s)	462, 2327
BanII	GRGCYC	1 cut(s)	540
BauI	CACGAG	1 cut(s)	999
BbsI	GAAGAC	5 cut(s)	334, 685, 1063, 1247, 2326
Bbv12I	GWGCWC	2 cut(s)	540, 1882
BbvCI	CCTCAGC	1 cut(s)	663
BceAI	ACGGC	2 cut(s)	600, 2136
BciT130I	CCWGG	3 cut(s)	95, 433, 573
BclI	TGATCA	1 cut(s)	2050
BcoDI	GTCTC	3 cut(s)	477, 1317, 2360
BcuI	ACTAGT	1 cut(s)	878
BfmI	CTRYAG	5 cut(s)	491, 1458, 1671, 1740, 1821
BfoI	RGCGCY	1 cut(s)	1447
BfrI	CTTAAG	1 cut(s)	341
BfuAI	ACCTGC	1 cut(s)	1570
BglII	AGATCT	1 cut(s)	1778
BmcAI	AGTACT	1 cut(s)	548
Bme1390I	CCNGG	3 cut(s)	95, 433, 573
Bme18I	GGWCC	1 cut(s)	1261
BmgBI	CACGTC	2 cut(s)	908, 925
BmgT120I	GGNCC	2 cut(s)	1261, 1300
BmiI	GGNNCC	2 cut(s)	92, 1262
BmrFI	CCNGG	3 cut(s)	95, 433, 573
BmsI	GCATC	7 cut(s)	67, 493, 793, 818, 1475, 1529, 2386
BmtI	GCTAGC	2 cut(s)	726, 1226
BoxI	GACNNNNGTC	1 cut(s)	1009
BpiI	GAAGAC	5 cut(s)	334, 685, 1063, 1247, 2326
BplI	GAGNNNNNCTC	2 cut(s)	1343, 1375
BpmI	CTGGAG	1 cut(s)	293
Bpu10I	CCTNAGC	1 cut(s)	663
BpuEI	CTTGAG	1 cut(s)	1661
BsaAI	YACGTR	2 cut(s)	1043, 1400
BsaBI	GATNNNNATC	2 cut(s)	790, 1338
BsaI	GGTCTC	1 cut(s)	477
BsaJI	CCNNGG	2 cut(s)	571, 834
BsaWI	WCCGGW	1 cut(s)	250
BsaXI	ACNNNNNCTCC	2 cut(s)	2253, 2283
Bsc4I	CCNNNNNNNGG	3 cut(s)	1874, 2036, 2225
Bse1I	ACTGG	3 cut(s)	1146, 1257, 1920
Bse3DI	GCAATG	3 cut(s)	718, 775, 997
Bse8I	GATNNNNATC	2 cut(s)	790, 1338
BseAI	TCCGGA	1 cut(s)	250
BseBI	CCWGG	3 cut(s)	95, 433, 573
BseDI	CCNNGG	2 cut(s)	571, 834
BseGI	GGATG	3 cut(s)	14, 1283, 1339
BseJI	GATNNNNATC	2 cut(s)	790, 1338
BseLI	CCNNNNNNNGG	3 cut(s)	1874, 2036, 2225
BseMI	GCAATG	3 cut(s)	718, 775, 997
BseMII	CTCAG	4 cut(s)	521, 654, 875, 2244
BseNI	ACTGG	3 cut(s)	1146, 1257, 1920
BseSI	GKGCMC	2 cut(s)	878, 1882
BseYI	CCCAGC	1 cut(s)	2314
BsgI	GTGCAG	2 cut(s)	519, 1944
BshFI	GGCC	6 cut(s)	462, 1302, 1358, 1659, 2059, 2327
BsiHKAI	GWGCWC	2 cut(s)	540, 1882
BsiSI	CCGG	1 cut(s)	251
BslFI	GGGAC	2 cut(s)	1274, 2241
BslI	CCNNNNNNNGG	3 cut(s)	1874, 2036, 2225
BsmAI	GTCTC	3 cut(s)	477, 1317, 2360
BsmFI	GGGAC	2 cut(s)	1274, 2241
BsmI	GAATGC	3 cut(s)	943, 1224, 2114
BsnI	GGCC	6 cut(s)	462, 1302, 1358, 1659, 2059, 2327
Bso31I	GGTCTC	1 cut(s)	477
Bsp1286I	GDGCHC	3 cut(s)	540, 878, 1882
Bsp13I	TCCGGA	1 cut(s)	250
Bsp1407I	TGTACA	3 cut(s)	358, 1795, 2072
Bsp143I	GATC	5 cut(s)	304, 577, 1778, 2024, 2050
Bsp19I	CCATGG	1 cut(s)	834
BspANI	GGCC	6 cut(s)	462, 1302, 1358, 1659, 2059, 2327
BspCNI	CTCAG	4 cut(s)	520, 655, 874, 2245
BspEI	TCCGGA	1 cut(s)	250
BspHI	TCATGA	1 cut(s)	933
BspLI	GGNNCC	2 cut(s)	92, 1262
BspMAI	CTGCAG	4 cut(s)	495, 1462, 1675, 1744
BspMI	ACCTGC	1 cut(s)	1570
BspOI	GCTAGC	2 cut(s)	726, 1226
BspTI	CTTAAG	1 cut(s)	341
BspTNI	GGTCTC	1 cut(s)	477
BsrBI	CCGCTC	2 cut(s)	1987, 2223
BsrDI	GCAATG	3 cut(s)	718, 775, 997
BsrGI	TGTACA	3 cut(s)	358, 1795, 2072
BsrI	ACTGG	3 cut(s)	1146, 1257, 1920
BssECI	CCNNGG	2 cut(s)	571, 834
BssMI	GATC	5 cut(s)	304, 577, 1778, 2024, 2050
BssNAI	GTATAC	1 cut(s)	2387
BssSI	CACGAG	1 cut(s)	999
BssT1I	CCWWGG	1 cut(s)	834
Bst1107I	GTATAC	1 cut(s)	2387
Bst2BI	CACGAG	1 cut(s)	999
Bst2UI	CCWGG	3 cut(s)	95, 433, 573
Bst4CI	ACNGT	5 cut(s)	234, 1060, 1495, 1956, 1969
Bst6I	CTCTTC	1 cut(s)	814
BstAFI	CTTAAG	1 cut(s)	341
BstAPI	GCANNNNNTGC	6 cut(s)	845, 1571, 1748, 1886, 1931, 2444
BstAUI	TGTACA	3 cut(s)	358, 1795, 2072
BstBAI	YACGTR	2 cut(s)	1043, 1400
BstC8I	GCNNGC	7 cut(s)	654, 724, 1224, 1356, 1647, 2174, 2445
BstDEI	CTNAG	8 cut(s)	369, 507, 663, 861, 1102, 1383, 2253, 2456
BstDSI	CCRYGG	1 cut(s)	834
BstEII	GGTNACC	1 cut(s)	2165
BstF5I	GGATG	3 cut(s)	14, 1283, 1339
BstH2I	RGCGCY	1 cut(s)	1447
BstHHI	GCGC	2 cut(s)	1446, 2438
BstKTI	GATC	5 cut(s)	307, 580, 1781, 2027, 2053
BstMAI	GTCTC	3 cut(s)	477, 1317, 2360
BstMBI	GATC	5 cut(s)	304, 577, 1778, 2024, 2050
BstNI	CCWGG	3 cut(s)	95, 433, 573
BstNSI	RCATGY	1 cut(s)	1886
BstPAI	GACNNNNGTC	1 cut(s)	1009
BstPI	GGTNACC	1 cut(s)	2165
BstSCI	CCNGG	3 cut(s)	93, 431, 571
BstSFI	CTRYAG	5 cut(s)	491, 1458, 1671, 1740, 1821
BstSLI	GKGCMC	2 cut(s)	878, 1882
BstSNI	TACGTA	1 cut(s)	1043
BstV2I	GAAGAC	5 cut(s)	334, 685, 1063, 1247, 2326
BstX2I	RGATCY	1 cut(s)	1778
BstYI	RGATCY	1 cut(s)	1778
BstZ17I	GTATAC	1 cut(s)	2387
BsuRI	GGCC	6 cut(s)	462, 1302, 1358, 1659, 2059, 2327
BtgI	CCRYGG	1 cut(s)	834
BtrI	CACGTC	2 cut(s)	908, 925
BtsCI	GGATG	3 cut(s)	14, 1283, 1339
BtsI	GCAGTG	1 cut(s)	1233
BtsIMutI	CAGTG	5 cut(s)	169, 1233, 1491, 1927, 1961
BveI	ACCTGC	1 cut(s)	1570
Cac8I	GCNNGC	7 cut(s)	654, 724, 1224, 1356, 1647, 2174, 2445
CaiI	CAGNNNCTG	1 cut(s)	1748
CciI	TCATGA	1 cut(s)	933
CfoI	GCGC	2 cut(s)	1446, 2438
Cfr13I	GGNCC	2 cut(s)	1261, 1300
CsiI	ACCWGGT	1 cut(s)	93
CspCI	CAANNNNNGTGG	4 cut(s)	21, 56, 145, 180
CviAII	CATG	5 cut(s)	835, 934, 1349, 1786, 1883
DdeI	CTNAG	8 cut(s)	369, 507, 663, 861, 1102, 1383, 2253, 2456
DpnI	GATC	5 cut(s)	306, 579, 1780, 2026, 2052
DpnII	GATC	5 cut(s)	304, 577, 1778, 2024, 2050
DrdI	GACNNNNNNGTC	1 cut(s)	929
DseDI	GACNNNNNNGTC	1 cut(s)	929
EaeI	YGGCCR	3 cut(s)	460, 2057, 2325
Eam1104I	CTCTTC	1 cut(s)	814
EarI	CTCTTC	1 cut(s)	814
EciI	GGCGGA	1 cut(s)	1212
Ecl136II	GAGCTC	1 cut(s)	538
Eco105I	TACGTA	1 cut(s)	1043
Eco130I	CCWWGG	1 cut(s)	834
Eco147I	AGGCCT	1 cut(s)	1358
Eco24I	GRGCYC	1 cut(s)	540
Eco31I	GGTCTC	1 cut(s)	477
Eco32I	GATATC	4 cut(s)	690, 1294, 1340, 1419
Eco47I	GGWCC	1 cut(s)	1261
Eco53kI	GAGCTC	1 cut(s)	538
Eco57I	CTGAAG	4 cut(s)	740, 1686, 2052, 2258
Eco91I	GGTNACC	1 cut(s)	2165
EcoICRI	GAGCTC	1 cut(s)	538
EcoO109I	RGGNCCY	2 cut(s)	1261, 1300
EcoO65I	GGTNACC	1 cut(s)	2165
EcoRII	CCWGG	3 cut(s)	93, 431, 571
EcoRV	GATATC	4 cut(s)	690, 1294, 1340, 1419
EcoT14I	CCWWGG	1 cut(s)	834
EcoT22I	ATGCAT	1 cut(s)	82
EcoT38I	GRGCYC	1 cut(s)	540
ErhI	CCWWGG	1 cut(s)	834
FaeI	CATG	5 cut(s)	838, 937, 1352, 1789, 1886
FalI	AAGNNNNNCTT	2 cut(s)	1185, 1217
FaqI	GGGAC	2 cut(s)	1274, 2241
FatI	CATG	5 cut(s)	834, 933, 1348, 1785, 1882
FauI	CCCGC	2 cut(s)	645, 2216
FauNDI	CATATG	2 cut(s)	192, 1234
FbaI	TGATCA	1 cut(s)	2050
FblI	GTMKAC	1 cut(s)	2386
FokI	GGATG	2 cut(s)	1290, 1346
FriOI	GRGCYC	1 cut(s)	540
GlaI	GCGC	2 cut(s)	1445, 2437
GsaI	CCCAGC	1 cut(s)	2318
GsuI	CTGGAG	1 cut(s)	293
HaeII	RGCGCY	1 cut(s)	1447
HaeIII	GGCC	6 cut(s)	462, 1302, 1358, 1659, 2059, 2327
HapII	CCGG	1 cut(s)	251
HhaI	GCGC	2 cut(s)	1446, 2438
Hin1II	CATG	5 cut(s)	838, 937, 1352, 1789, 1886
Hin6I	GCGC	2 cut(s)	1444, 2436
HinP1I	GCGC	2 cut(s)	1444, 2436
HincII	GTYRAC	1 cut(s)	831
HindII	GTYRAC	1 cut(s)	831
HindIII	AAGCTT	1 cut(s)	2441
HpaII	CCGG	1 cut(s)	251
HphI	GGTGA	7 cut(s)	74, 585, 974, 1183, 1295, 1775, 2019
Hpy188I	TCNGA	9 cut(s)	145, 238, 510, 582, 607, 1423, 1778, 1978, 2220
HpyAV	CCTTC	9 cut(s)	140, 340, 1086, 1187, 1628, 1707, 2002, 2123, 2298
HpyCH4III	ACNGT	5 cut(s)	234, 1060, 1495, 1956, 1969
HpyCH4IV	ACGT	6 cut(s)	159, 907, 924, 1042, 1399, 1801
HpyF3I	CTNAG	8 cut(s)	369, 507, 663, 861, 1102, 1383, 2253, 2456
HpySE526I	ACGT	6 cut(s)	159, 907, 924, 1042, 1399, 1801
Hsp92II	CATG	5 cut(s)	838, 937, 1352, 1789, 1886
HspAI	GCGC	2 cut(s)	1444, 2436
Kpn2I	TCCGGA	1 cut(s)	250
Ksp22I	TGATCA	1 cut(s)	2050
Kzo9I	GATC	5 cut(s)	304, 577, 1778, 2024, 2050
LmnI	GCTCC	6 cut(s)	114, 1120, 1984, 2050, 2290, 2350
LweI	GCATC	7 cut(s)	67, 493, 793, 818, 1475, 1529, 2386
MabI	ACCWGGT	1 cut(s)	93
MaeII	ACGT	6 cut(s)	159, 907, 924, 1042, 1399, 1801
MaeIII	GTNAC	7 cut(s)	1129, 1283, 1489, 1498, 1989, 2165, 2243
MalI	GATC	5 cut(s)	306, 579, 1780, 2026, 2052
MbiI	CCGCTC	2 cut(s)	1987, 2223
MboI	GATC	5 cut(s)	304, 577, 1778, 2024, 2050
MfeI	CAATTG	1 cut(s)	1530
MflI	RGATCY	1 cut(s)	1778
MhlI	GDGCHC	3 cut(s)	540, 878, 1882
MlsI	TGGCCA	2 cut(s)	462, 2327
MluNI	TGGCCA	2 cut(s)	462, 2327
MlyI	GAGTC	3 cut(s)	131, 324, 1319
Mox20I	TGGCCA	2 cut(s)	462, 2327
Mph1103I	ATGCAT	1 cut(s)	82
MroI	TCCGGA	1 cut(s)	250
MscI	TGGCCA	2 cut(s)	462, 2327
MseI	TTAA	6 cut(s)	57, 342, 867, 1365, 2136, 2214
MslI	CAYNNNNRTG	2 cut(s)	1332, 1496
Msp20I	TGGCCA	2 cut(s)	462, 2327
MspA1I	CMGCKG	3 cut(s)	470, 1515, 1757
MspCI	CTTAAG	1 cut(s)	341
MspI	CCGG	1 cut(s)	251
MspR9I	CCNGG	3 cut(s)	95, 433, 573
MunI	CAATTG	1 cut(s)	1530
Mva1269I	GAATGC	3 cut(s)	943, 1224, 2114
MvaI	CCWGG	3 cut(s)	95, 433, 573
NcoI	CCATGG	1 cut(s)	834
NdeI	CATATG	2 cut(s)	192, 1234
NdeII	GATC	5 cut(s)	304, 577, 1778, 2024, 2050
NheI	GCTAGC	2 cut(s)	722, 1222
NlaIII	CATG	5 cut(s)	838, 937, 1352, 1789, 1886
NlaIV	GGNNCC	2 cut(s)	92, 1262
NmeAIII	GCCGAG	1 cut(s)	1834
NmuCI	GTSAC	4 cut(s)	1283, 1489, 1498, 2243
NsiI	ATGCAT	1 cut(s)	82
NspI	RCATGY	1 cut(s)	1886
OliI	CACNNNNGTG	1 cut(s)	1496
PagI	TCATGA	1 cut(s)	933
PceI	AGGCCT	1 cut(s)	1358
PctI	GAATGC	3 cut(s)	943, 1224, 2114
PfeI	GAWTC	7 cut(s)	173, 284, 365, 440, 1174, 1424, 2108
PflFI	GACNNNGTC	1 cut(s)	1613
PleI	GAGTC	3 cut(s)	131, 324, 1318
PpsI	GAGTC	3 cut(s)	131, 324, 1318
Ppu21I	YACGTR	2 cut(s)	1043, 1400
PpuMI	RGGWCCY	1 cut(s)	1261
PshAI	GACNNNNGTC	1 cut(s)	1009
Psp124BI	GAGCTC	1 cut(s)	540
Psp1406I	AACGTT	1 cut(s)	1801
Psp5II	RGGWCCY	1 cut(s)	1261
Psp6I	CCWGG	3 cut(s)	93, 431, 571
PspEI	GGTNACC	1 cut(s)	2165
PspFI	CCCAGC	1 cut(s)	2314
PspGI	CCWGG	3 cut(s)	93, 431, 571
PspN4I	GGNNCC	2 cut(s)	92, 1262
PspPI	GGNCC	2 cut(s)	1261, 1300
PspPPI	RGGWCCY	1 cut(s)	1261
PstI	CTGCAG	4 cut(s)	495, 1462, 1675, 1744
PstNI	CAGNNNCTG	1 cut(s)	1748
PsuI	RGATCY	1 cut(s)	1778
PsyI	GACNNNGTC	1 cut(s)	1613
RseI	CAYNNNNRTG	2 cut(s)	1332, 1496
SacI	GAGCTC	1 cut(s)	540
SaqAI	TTAA	6 cut(s)	57, 342, 867, 1365, 2136, 2214
Sau3AI	GATC	5 cut(s)	304, 577, 1778, 2024, 2050
Sau96I	GGNCC	2 cut(s)	1261, 1300
ScaI	AGTACT	1 cut(s)	548
SchI	GAGTC	3 cut(s)	131, 324, 1319
ScrFI	CCNGG	3 cut(s)	95, 433, 573
SduI	GDGCHC	3 cut(s)	540, 878, 1882
SexAI	ACCWGGT	1 cut(s)	93
SfaNI	GCATC	7 cut(s)	67, 493, 793, 818, 1475, 1529, 2386
SfcI	CTRYAG	5 cut(s)	491, 1458, 1671, 1740, 1821
SinI	GGWCC	1 cut(s)	1261
SmiMI	CAYNNNNRTG	2 cut(s)	1332, 1496
SmlI	CTYRAG	2 cut(s)	341, 1640
SmoI	CTYRAG	2 cut(s)	341, 1640
SnaBI	TACGTA	1 cut(s)	1043
SpeI	ACTAGT	1 cut(s)	878
SseBI	AGGCCT	1 cut(s)	1358
SspI	AATATT	1 cut(s)	855
SstI	GAGCTC	1 cut(s)	540
StuI	AGGCCT	1 cut(s)	1358
StyD4I	CCNGG	3 cut(s)	93, 431, 571
StyI	CCWWGG	1 cut(s)	834
TaaI	ACNGT	5 cut(s)	234, 1060, 1495, 1956, 1969
TaiI	ACGT	6 cut(s)	162, 910, 927, 1045, 1402, 1804
TaqI	TCGA	3 cut(s)	1296, 1872, 2419
TatI	WGTACW	6 cut(s)	358, 546, 1693, 1795, 2072, 2101
TauI	GCSGC	2 cut(s)	1757, 2236
TfiI	GAWTC	7 cut(s)	173, 284, 365, 440, 1174, 1424, 2108
Tru1I	TTAA	6 cut(s)	57, 342, 867, 1365, 2136, 2214
Tru9I	TTAA	6 cut(s)	57, 342, 867, 1365, 2136, 2214
TscAI	CASTG	5 cut(s)	169, 1233, 1498, 1927, 1961
TseFI	GTSAC	4 cut(s)	1283, 1489, 1498, 2243
Tsp45I	GTSAC	4 cut(s)	1283, 1489, 1498, 2243
TspDTI	ATGAA	9 cut(s)	60, 786, 950, 1101, 1536, 1542, 1774, 2313, 2326
TspGWI	ACGGA	5 cut(s)	7, 1477, 1646, 2281, 2404
TspRI	CASTG	5 cut(s)	169, 1233, 1498, 1927, 1961
Tth111I	GACNNNGTC	1 cut(s)	1613
Vha464I	CTTAAG	1 cut(s)	341
VneI	GTGCAC	1 cut(s)	1878
VpaK11BI	GGWCC	1 cut(s)	1261
XapI	RAATTY	4 cut(s)	218, 1843, 2264, 2302
XceI	RCATGY	1 cut(s)	1886
XmiI	GTMKAC	1 cut(s)	2386
ZrmI	AGTACT	1 cut(s)	548
Zsp2I	ATGCAT	1 cut(s)	82

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.