MD07G1196400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009308
Physical Location & Seq
Forward (+)
27476139 .. 27479746
3608 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1196400.v1.1.491

Sequence Viewer

Length: 927 bp
ATGAAGGCCAACAAGGCAACCGTCTTAACATTTGCCGAGAAATGCAAGAGTATACTGTCTTCCAACTGGCAAGGGCAACTCAATACCATCAAAGCTGATGCTAAAGGAAGCAAGGAGGACATATACACATCAAAGGTTAAGTACATCTGCAAAAGAGGGAAGCCATACCTTTGGGTGCCCGAAAAGGAGTTGCATAATGTGAATGTAGTTGTTGATGAGCGCTGTTCATTTGCTGTCACCAATCCCATCCCAGGTCCACTGGCAAGCTTACTTAGATCATTAAGAAAGACACCGCCTCGAATTGCCCTGACTGGCGAAGTTGTGCCTCTAACTGAGGAAAAGGTTCAATCTGCAACAAAAGTCCTTAGAGAAGTCATACGGTCAGAACAGAAAGCAGTGAGTGAGTCCAGTTATACAGTTTCAGGTATATTAAGTTCTTCTGATCATAGCTGTACATCACGAAGCGAAAGCCTTAAGGAGCTCTTGGAAGGCGATGAAAACTACATAGTTTACAAGTTCAATCAAAGATCGTGTATGTATGTTGATGGCAGTGGAGACACTCATAAGATTGATACAGAAGATATGGCTGCGTCTAAAGCAGATTTGTTAGCTCCTTTGTCTGCCAAACTCATTGATGGGATAAATCAAAGTGCAGCAAGACGTAGAGCGCTAATGCTTTTCTGCTGTTCATACTTGAATGCAAATGCAAAGGATGCATACATGCTGTCTATCGACCGCAAGGGGTTTGAGGTGTTGAGCAAGGTTCCGAGTCCTGGGTTTAAGGATGGATTTGGCCAGTTCGAGTGGAAGGAATTTAGACTGTCATTGAAGGAAGAGGCACGTGACGTTGAAGCATTCTGCCGTAAACTTGTGGAAATGGAAGAGGAGGCTGTAAAGGAGGTTTCAGGCCATAGTGGTTTAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2470 Pyrid_oxidase_2

Protein Analysis

309

Amino Acids

34.24

Weight (kDa)

8.3

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CREG_beta-barrel PF13883 11 - 121 3.7e-06 CREG, beta-barrel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 175
AccI GTMKAC 1 cut(s) 52
AciI CCGC 2 cut(s) 293, 736
AcoI YGGCCR 1 cut(s) 793
AcsI RAATTY 1 cut(s) 812
AcvI CACGTG 1 cut(s) 842
AfaI GTAC 2 cut(s) 143, 454
AfeI AGCGCT 2 cut(s) 221, 669
AfiI CCNNNNNNNGG 2 cut(s) 251, 773
AflII CTTAAG 1 cut(s) 473
AgsI TTSAA 5 cut(s) 347, 520, 697, 829, 851
AjnI CCWGG 2 cut(s) 250, 772
AluBI AGCT 5 cut(s) 95, 267, 450, 481, 611
AluI AGCT 5 cut(s) 95, 267, 450, 481, 611
Alw21I GWGCWC 1 cut(s) 483
Alw26I GTCTC 1 cut(s) 549
Aor51HI AGCGCT 2 cut(s) 221, 669
AoxI GGCC 3 cut(s) 6, 793, 907
ApeKI GCWGC 2 cut(s) 587, 653
ApoI RAATTY 1 cut(s) 812
Asp700I GAANNNNTTC 1 cut(s) 342
AspLEI GCGC 2 cut(s) 222, 670
AspS9I GGNCC 1 cut(s) 254
AsuHPI GGTGA 1 cut(s) 229
AvaII GGWCC 1 cut(s) 254
BaeGI GKGCMC 1 cut(s) 180
BalI TGGCCA 1 cut(s) 795
BanI GGYRCC 1 cut(s) 175
BanII GRGCYC 1 cut(s) 483
BarI GAAGNNNNNNTAC 2 cut(s) 43, 75
BbrPI CACGTG 1 cut(s) 842
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 1 cut(s) 483
BbvI GCAGC 2 cut(s) 574, 665
BccI CCATC 5 cut(s) 95, 254, 539, 629, 779
BceAI ACGGC 1 cut(s) 846
BciT130I CCWGG 2 cut(s) 252, 774
BclI TGATCA 1 cut(s) 442
BcoDI GTCTC 1 cut(s) 549
BfoI RGCGCY 2 cut(s) 223, 671
BfrI CTTAAG 1 cut(s) 473
BglI GCCNNNNNGGC 1 cut(s) 14
BisI GCNGC 2 cut(s) 588, 654
BlsI GCNGC 2 cut(s) 589, 655
Bme1390I CCNGG 2 cut(s) 252, 774
Bme18I GGWCC 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 254
BmiI GGNNCC 2 cut(s) 177, 765
BmrFI CCNGG 2 cut(s) 252, 774
BmsI GCATC 2 cut(s) 88, 703
BpiI GAAGAC 1 cut(s) 51
BsaAI YACGTR 1 cut(s) 842
BsaJI CCNNGG 2 cut(s) 250, 773
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 773
Bse1I ACTGG 5 cut(s) 71, 264, 316, 408, 796
BseBI CCWGG 2 cut(s) 252, 774
BseDI CCNNGG 2 cut(s) 250, 773
BseGI GGATG 3 cut(s) 246, 718, 790
BseLI CCNNNNNNNGG 2 cut(s) 251, 773
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 5 cut(s) 71, 264, 316, 408, 796
BseRI GAGGAG 1 cut(s) 899
BseSI GKGCMC 1 cut(s) 180
BseXI GCAGC 2 cut(s) 574, 665
BsgI GTGCAG 1 cut(s) 672
Bsh1285I CGRYCG 1 cut(s) 736
BshFI GGCC 3 cut(s) 8, 795, 909
BshNI GGYRCC 1 cut(s) 175
BsiEI CGRYCG 1 cut(s) 736
BsiHKAI GWGCWC 1 cut(s) 483
BslI CCNNNNNNNGG 2 cut(s) 251, 773
BsmAI GTCTC 1 cut(s) 549
BsmI GAATGC 2 cut(s) 703, 854
BsnI GGCC 3 cut(s) 8, 795, 909
Bsp1286I GDGCHC 2 cut(s) 180, 483
Bsp1407I TGTACA 1 cut(s) 452
Bsp143I GATC 3 cut(s) 275, 442, 527
BspACI CCGC 2 cut(s) 293, 736
BspANI GGCC 3 cut(s) 8, 795, 909
BspCNI CTCAG 1 cut(s) 325
BspLI GGNNCC 2 cut(s) 177, 765
BspT107I GGYRCC 1 cut(s) 175
BspTI CTTAAG 1 cut(s) 473
BsrGI TGTACA 1 cut(s) 452
BsrI ACTGG 5 cut(s) 71, 264, 316, 408, 796
BssECI CCNNGG 2 cut(s) 250, 773
BssMI GATC 3 cut(s) 275, 442, 527
BssNAI GTATAC 1 cut(s) 53
Bst1107I GTATAC 1 cut(s) 53
Bst2UI CCWGG 2 cut(s) 252, 774
Bst4CI ACNGT 5 cut(s) 22, 57, 381, 418, 822
Bst6I CTCTTC 2 cut(s) 828, 876
BstAFI CTTAAG 1 cut(s) 473
BstAPI GCANNNNNTGC 1 cut(s) 713
BstAUI TGTACA 1 cut(s) 452
BstBAI YACGTR 1 cut(s) 842
BstC8I GCNNGC 1 cut(s) 265
BstDEI CTNAG 3 cut(s) 272, 333, 365
BstF5I GGATG 3 cut(s) 246, 718, 790
BstH2I RGCGCY 2 cut(s) 223, 671
BstHHI GCGC 2 cut(s) 222, 670
BstKTI GATC 3 cut(s) 278, 445, 530
BstMAI GTCTC 1 cut(s) 549
BstMBI GATC 3 cut(s) 275, 442, 527
BstMCI CGRYCG 1 cut(s) 736
BstMWI GCNNNNNNNGC 3 cut(s) 14, 596, 713
BstNI CCWGG 2 cut(s) 252, 774
BstNSI RCATGY 1 cut(s) 724
BstSCI CCNGG 2 cut(s) 250, 772
BstSLI GKGCMC 1 cut(s) 180
BstV1I GCAGC 2 cut(s) 574, 665
BstV2I GAAGAC 1 cut(s) 51
BstXI CCANNNNNNTGG 1 cut(s) 171
BstZ17I GTATAC 1 cut(s) 53
BsuRI GGCC 3 cut(s) 8, 795, 909
BtgZI GCGATG 1 cut(s) 507
BtsCI GGATG 3 cut(s) 246, 718, 790
BtsI GCAGTG 2 cut(s) 402, 556
BtsIMutI CAGTG 3 cut(s) 257, 402, 556
Cac8I GCNNGC 1 cut(s) 265
CfoI GCGC 2 cut(s) 222, 670
Cfr13I GGNCC 1 cut(s) 254
CseI GACGC 1 cut(s) 579
Csp6I GTAC 2 cut(s) 142, 453
CviAII CATG 1 cut(s) 721
CviQI GTAC 2 cut(s) 142, 453
DdeI CTNAG 3 cut(s) 272, 333, 365
DpnI GATC 3 cut(s) 277, 444, 529
DpnII GATC 3 cut(s) 275, 442, 527
EaeI YGGCCR 1 cut(s) 793
Eam1104I CTCTTC 2 cut(s) 828, 876
EarI CTCTTC 2 cut(s) 828, 876
Ecl136II GAGCTC 1 cut(s) 481
Eco24I GRGCYC 1 cut(s) 483
Eco47I GGWCC 1 cut(s) 254
Eco47III AGCGCT 2 cut(s) 221, 669
Eco53kI GAGCTC 1 cut(s) 481
Eco72I CACGTG 1 cut(s) 842
EcoICRI GAGCTC 1 cut(s) 481
EcoRII CCWGG 2 cut(s) 250, 772
EcoT22I ATGCAT 1 cut(s) 718
EcoT38I GRGCYC 1 cut(s) 483
FaeI CATG 1 cut(s) 724
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FatI CATG 1 cut(s) 720
FbaI TGATCA 1 cut(s) 442
FblI GTMKAC 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 588, 654
FokI GGATG 3 cut(s) 233, 725, 797
FriOI GRGCYC 1 cut(s) 483
Fsp4HI GCNGC 2 cut(s) 588, 654
GlaI GCGC 2 cut(s) 221, 669
GluI GCNGC 2 cut(s) 588, 654
HaeII RGCGCY 2 cut(s) 223, 671
HaeIII GGCC 3 cut(s) 8, 795, 909
HgaI GACGC 1 cut(s) 579
HhaI GCGC 2 cut(s) 222, 670
Hin1II CATG 1 cut(s) 724
Hin6I GCGC 2 cut(s) 220, 668
HinP1I GCGC 2 cut(s) 220, 668
HindIII AAGCTT 1 cut(s) 265
HinfI GANTC 2 cut(s) 404, 769
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 4 cut(s) 53, 257, 511, 866
Hpy188I TCNGA 3 cut(s) 385, 442, 768
Hpy188III TCNNGA 1 cut(s) 459
Hpy8I GTNNAC 4 cut(s) 53, 257, 511, 866
HpyAV CCTTC 3 cut(s) 482, 802, 823
HpyCH4III ACNGT 5 cut(s) 22, 57, 381, 418, 822
HpyCH4IV ACGT 3 cut(s) 661, 841, 846
HpyCH4V TGCA 8 cut(s) 45, 150, 193, 353, 653, 701, 707, 716
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 596, 713
HpyF3I CTNAG 3 cut(s) 272, 333, 365
HpySE526I ACGT 3 cut(s) 661, 841, 846
Hsp92II CATG 1 cut(s) 724
HspAI GCGC 2 cut(s) 220, 668
Ksp22I TGATCA 1 cut(s) 442
Kzo9I GATC 3 cut(s) 275, 442, 527
LmnI GCTCC 2 cut(s) 478, 616
Lsp1109I GCAGC 2 cut(s) 574, 665
LweI GCATC 2 cut(s) 88, 703
MaeII ACGT 3 cut(s) 661, 841, 846
MaeIII GTNAC 2 cut(s) 235, 842
MalI GATC 3 cut(s) 277, 444, 529
MboI GATC 3 cut(s) 275, 442, 527
MboII GAAGA 5 cut(s) 51, 429, 590, 845, 893
MhlI GDGCHC 2 cut(s) 180, 483
MlsI TGGCCA 1 cut(s) 795
MluCI AATT 2 cut(s) 300, 812
MluNI TGGCCA 1 cut(s) 795
MlyI GAGTC 2 cut(s) 413, 778
MmeI TCCRAC 1 cut(s) 87
Mox20I TGGCCA 1 cut(s) 795
Mph1103I ATGCAT 1 cut(s) 718
MroXI GAANNNNTTC 1 cut(s) 342
MscI TGGCCA 1 cut(s) 795
MseI TTAA 6 cut(s) 26, 138, 281, 431, 474, 780
Msp20I TGGCCA 1 cut(s) 795
MspCI CTTAAG 1 cut(s) 473
MspR9I CCNGG 2 cut(s) 252, 774
Mva1269I GAATGC 2 cut(s) 703, 854
MvaI CCWGG 2 cut(s) 252, 774
MwoI GCNNNNNNNGC 3 cut(s) 14, 596, 713
NdeII GATC 3 cut(s) 275, 442, 527
NlaIII CATG 1 cut(s) 724
NlaIV GGNNCC 2 cut(s) 177, 765
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 2 cut(s) 235, 842
NsiI ATGCAT 1 cut(s) 718
NspI RCATGY 1 cut(s) 724
PctI GAATGC 2 cut(s) 703, 854
PdmI GAANNNNTTC 1 cut(s) 342
PkrI GCNGC 2 cut(s) 589, 655
PleI GAGTC 2 cut(s) 412, 777
PmaCI CACGTG 1 cut(s) 842
PmlI CACGTG 1 cut(s) 842
PpsI GAGTC 2 cut(s) 412, 777
Ppu21I YACGTR 1 cut(s) 842
Psp124BI GAGCTC 1 cut(s) 483
Psp6I CCWGG 2 cut(s) 250, 772
PspCI CACGTG 1 cut(s) 842
PspGI CCWGG 2 cut(s) 250, 772
PspN4I GGNNCC 2 cut(s) 177, 765
PspPI GGNCC 1 cut(s) 254
PsrI GAACNNNNNNTAC 2 cut(s) 418, 450
RsaI GTAC 2 cut(s) 143, 454
RsaNI GTAC 2 cut(s) 142, 453
SacI GAGCTC 1 cut(s) 483
SaqAI TTAA 6 cut(s) 26, 138, 281, 431, 474, 780
SatI GCNGC 2 cut(s) 588, 654
Sau3AI GATC 3 cut(s) 275, 442, 527
Sau96I GGNCC 1 cut(s) 254
SchI GAGTC 2 cut(s) 413, 778
ScrFI CCNGG 2 cut(s) 252, 774
SduI GDGCHC 2 cut(s) 180, 483
SfaNI GCATC 2 cut(s) 88, 703
SinI GGWCC 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 2 cut(s) 300, 812
SsiI CCGC 2 cut(s) 293, 736
SstI GAGCTC 1 cut(s) 483
StyD4I CCNGG 2 cut(s) 250, 772
TaaI ACNGT 5 cut(s) 22, 57, 381, 418, 822
TaiI ACGT 3 cut(s) 664, 844, 849
TaqI TCGA 3 cut(s) 298, 732, 801
TasI AATT 2 cut(s) 300, 812
TatI WGTACW 2 cut(s) 141, 452
Tru1I TTAA 6 cut(s) 26, 138, 281, 431, 474, 780
Tru9I TTAA 6 cut(s) 26, 138, 281, 431, 474, 780
TscAI CASTG 3 cut(s) 264, 402, 556
TseFI GTSAC 2 cut(s) 235, 842
TseI GCWGC 2 cut(s) 587, 653
Tsp45I GTSAC 2 cut(s) 235, 842
TspDTI ATGAA 4 cut(s) 17, 216, 510, 678
TspRI CASTG 3 cut(s) 264, 402, 556
Vha464I CTTAAG 1 cut(s) 473
VpaK11BI GGWCC 1 cut(s) 254
XapI RAATTY 1 cut(s) 812
XceI RCATGY 1 cut(s) 724
XmiI GTMKAC 1 cut(s) 52
XmnI GAANNNNTTC 1 cut(s) 342
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.