MD07G1203600.v1.1

Tropinone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008147
Physical Location & Seq
Forward (+)
28127894 .. 28128829
936 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1203600.v1.1.491

Sequence Viewer

Length: 390 bp
ATGAGTCTTAAATCCAGATGTGGTAGGGGAAGAAAGAAAGAGATAAAGAGAAACAAAGAAGAGAAGAAAAAGAAAGGAATGTATTTTCCCAGCGGCAAAGAGAAAAAGAGCTGTCGCCATCATGTGGTGTTTGGTTGGAGTTGCATTAGACTGGGTTGGGTGAGGACTTTCGTGCTAGTTGCTAGCTGGTTTCAGTTGCTGGATAACAAGGAGTTTTTGGAAGAGAGAACGTCGCGAACTCCTCTTGAGCGCATTGCAGAGCCTGGGGAAGTCTCATCTCTGGTAGCATTCCTTTGCCTGCCGGCTGCTTCGTACATCACCGGTCAGATTATTTCTGTGGACGGAGGAATGACTGTAAACAGTTTCAACCCTACTAGCCGGCCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

130

Amino Acids

14.73

Weight (kDa)

9.97

Isoelectric Point (pI)

50.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short_C2 PF13561 65 - 118 9.1e-13 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 124
AccII CGCG 1 cut(s) 235
AciI CCGC 1 cut(s) 93
AcoI YGGCCR 1 cut(s) 380
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 1 cut(s) 124
AgeI ACCGGT 1 cut(s) 320
AgsI TTSAA 1 cut(s) 367
AjnI CCWGG 1 cut(s) 262
AluBI AGCT 2 cut(s) 111, 186
AluI AGCT 2 cut(s) 111, 186
Alw26I GTCTC 1 cut(s) 277
AlwNI CAGNNNCTG 2 cut(s) 199, 263
AoxI GGCC 1 cut(s) 380
ApeKI GCWGC 1 cut(s) 305
AsiGI ACCGGT 1 cut(s) 320
AspLEI GCGC 1 cut(s) 252
AsuHPI GGTGA 2 cut(s) 172, 310
AsuNHI GCTAGC 1 cut(s) 182
BbvI GCAGC 1 cut(s) 292
BccI CCATC 1 cut(s) 126
BciT130I CCWGG 1 cut(s) 264
BcoDI GTCTC 1 cut(s) 277
BfaI CTAG 3 cut(s) 176, 183, 375
BisI GCNGC 2 cut(s) 94, 306
BlsI GCNGC 2 cut(s) 95, 307
Bme1390I CCNGG 1 cut(s) 264
BmrFI CCNGG 1 cut(s) 264
BmrI ACTGGG 1 cut(s) 161
BmtI GCTAGC 1 cut(s) 186
BmuI ACTGGG 1 cut(s) 161
BpuEI CTTGAG 1 cut(s) 266
BsaJI CCNNGG 1 cut(s) 263
BsaWI WCCGGW 1 cut(s) 320
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse118I RCCGGY 3 cut(s) 301, 320, 378
Bse1I ACTGG 1 cut(s) 156
Bse3DI GCAATG 1 cut(s) 252
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 263
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMI GCAATG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 156
BseRI GAGGAG 1 cut(s) 231
BseXI GCAGC 1 cut(s) 292
BseYI CCCAGC 1 cut(s) 89
Bsh1236I CGCG 1 cut(s) 235
BshFI GGCC 1 cut(s) 382
BshTI ACCGGT 1 cut(s) 320
BsiSI CCGG 3 cut(s) 302, 321, 379
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 1 cut(s) 277
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 1 cut(s) 382
Bsp68I TCGCGA 1 cut(s) 235
BspACI CCGC 1 cut(s) 93
BspANI GGCC 1 cut(s) 382
BspFNI CGCG 1 cut(s) 235
BspOI GCTAGC 1 cut(s) 186
BsrDI GCAATG 1 cut(s) 252
BsrFI RCCGGY 3 cut(s) 301, 320, 378
BsrI ACTGG 1 cut(s) 156
BssAI RCCGGY 3 cut(s) 301, 320, 378
BssECI CCNNGG 1 cut(s) 263
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 2 cut(s) 355, 362
Bst6I CTCTTC 2 cut(s) 54, 216
BstC8I GCNNGC 4 cut(s) 184, 299, 303, 380
BstFNI CGCG 1 cut(s) 235
BstHHI GCGC 1 cut(s) 252
BstMAI GTCTC 1 cut(s) 277
BstNI CCWGG 1 cut(s) 264
BstSCI CCNGG 1 cut(s) 262
BstUI CGCG 1 cut(s) 235
BstV1I GCAGC 1 cut(s) 292
BsuRI GGCC 1 cut(s) 382
BtuMI TCGCGA 1 cut(s) 235
Cac8I GCNNGC 4 cut(s) 184, 299, 303, 380
CaiI CAGNNNCTG 2 cut(s) 199, 263
CfoI GCGC 1 cut(s) 252
Cfr10I RCCGGY 3 cut(s) 301, 320, 378
Csp6I GTAC 1 cut(s) 313
CspAI ACCGGT 1 cut(s) 320
CviAII CATG 1 cut(s) 122
CviJI RGCY 6 cut(s) 111, 186, 262, 305, 378, 382
CviKI_1 RGCY 6 cut(s) 111, 186, 262, 305, 378, 382
CviQI GTAC 1 cut(s) 313
EaeI YGGCCR 1 cut(s) 380
Eam1104I CTCTTC 2 cut(s) 54, 216
EarI CTCTTC 2 cut(s) 54, 216
EcoRII CCWGG 1 cut(s) 262
FaeI CATG 1 cut(s) 125
FaiI YATR 1 cut(s) 123
FatI CATG 1 cut(s) 121
Fnu4HI GCNGC 2 cut(s) 94, 306
Fsp4HI GCNGC 2 cut(s) 94, 306
FspBI CTAG 3 cut(s) 176, 183, 375
GlaI GCGC 1 cut(s) 251
GluI GCNGC 2 cut(s) 94, 306
GsaI CCCAGC 1 cut(s) 93
HaeIII GGCC 1 cut(s) 382
HapII CCGG 3 cut(s) 302, 321, 379
HhaI GCGC 1 cut(s) 252
Hin1II CATG 1 cut(s) 125
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HinfI GANTC 1 cut(s) 4
HpaII CCGG 3 cut(s) 302, 321, 379
HphI GGTGA 2 cut(s) 172, 310
Hpy166II GTNNAC 2 cut(s) 340, 358
Hpy188I TCNGA 1 cut(s) 327
Hpy188III TCNNGA 3 cut(s) 15, 234, 245
Hpy8I GTNNAC 2 cut(s) 340, 358
Hpy99I CGWCG 1 cut(s) 235
HpyCH4III ACNGT 2 cut(s) 355, 362
HpyCH4IV ACGT 1 cut(s) 230
HpyCH4V TGCA 2 cut(s) 144, 257
HpySE526I ACGT 1 cut(s) 230
Hsp92II CATG 1 cut(s) 125
HspAI GCGC 1 cut(s) 250
KroI GCCGGC 2 cut(s) 301, 378
KroNI GCCGGC 2 cut(s) 303, 380
Lsp1109I GCAGC 1 cut(s) 292
MaeI CTAG 3 cut(s) 176, 183, 375
MaeII ACGT 1 cut(s) 230
MboII GAAGA 4 cut(s) 42, 71, 76, 233
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 116
MnlI CCTC 3 cut(s) 156, 252, 338
MroNI GCCGGC 2 cut(s) 301, 378
MseI TTAA 1 cut(s) 9
MspA1I CMGCKG 1 cut(s) 93
MspI CCGG 3 cut(s) 302, 321, 379
MspR9I CCNGG 1 cut(s) 264
Mva1269I GAATGC 1 cut(s) 287
MvaI CCWGG 1 cut(s) 264
MvnI CGCG 1 cut(s) 235
NaeI GCCGGC 2 cut(s) 303, 380
NgoMIV GCCGGC 2 cut(s) 301, 378
NheI GCTAGC 1 cut(s) 182
NlaIII CATG 1 cut(s) 125
NruI TCGCGA 1 cut(s) 235
PctI GAATGC 1 cut(s) 287
PdiI GCCGGC 2 cut(s) 303, 380
PflMI CCANNNNNTGG 1 cut(s) 124
PinAI ACCGGT 1 cut(s) 320
PkrI GCNGC 2 cut(s) 95, 307
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 262
PspFI CCCAGC 1 cut(s) 89
PspGI CCWGG 1 cut(s) 262
PstNI CAGNNNCTG 2 cut(s) 199, 263
RruI TCGCGA 1 cut(s) 235
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 2 cut(s) 94, 306
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 1 cut(s) 264
SetI ASST 3 cut(s) 113, 188, 233
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
SsiI CCGC 1 cut(s) 93
SspMI CTAG 3 cut(s) 176, 183, 375
StyD4I CCNGG 1 cut(s) 262
TaaI ACNGT 2 cut(s) 355, 362
TaiI ACGT 1 cut(s) 233
TauI GCSGC 1 cut(s) 96
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TseI GCWGC 1 cut(s) 305
TspGWI ACGGA 1 cut(s) 357
Van91I CCANNNNNTGG 1 cut(s) 124
XspI CTAG 3 cut(s) 176, 183, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.