Rorug03G0249300

Tropinone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0008147
Physical Location & Seq
Reverse (-)
23454755 .. 23460259
5505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0249300.1

Sequence Viewer

Length: 1302 bp
ATGAAAGCATGTGTTATAATGCACAACATGATTATTGAAGATGAACGTTCAGAACGCATTGCACAAGATGATCTTGAAACTCTAGCAAGGCAATATGCTAAACCAGATATGGAAATTGATGATGTTTTGGTTTCACGTAATCACACAATAGAGTTTGAGGATTTTATCAGCAATCATTTGCGAATTCGTGACAATGGAACACATAGTATGCTCCAAGCAGATCTAGTTGAGCACATATGGCAATGCACTTGGCTAGAATCAGGGGGCAAGATCTCTGTTAAGCTTATTAAATACGAAAATAATGAAAACACAGAAAAAAGAGCTGACAGCTTGGTCTACAAGGTATTGAGAGGAATTAGGACAGATGCATTGAAGCTGCTTGTGATGCATAAGAGACCAATTCCTCGTGTTTCCACAACAATTGGTGCTTGGCTGAACATATTTCAGTTTCTTGTTGTTACATCCATATGCACCAACTGCGCCCTTCTAGAACCTGCTTGGGTACGAGCGAACCGTGTGAAAAGAGCAACAGAGGCTGAGTACATGTGCTCCAAACAGCTTTTGAGGAATATTTTTGGAGATAGGGCAGCTGGTACTAGTGCAGTTCCCAGTTTTTTCACTCAAGACTCAGAGCATGAAAATGCTGATGAGGTCTGTCAAGTGGCTAACAAAAGGACTCCAAGAGGTCCCGACTTTAATGTAGAGGAGGATAGACTTCTTATAAGTGCTTGGTTGAATACTAGTATGGATTCAATACAAGGCAATGATCAAAAGCTGCAAATTTTTTGGGGGAAAATTACAGAGTACTATCATCAGTACAAGTCCTTTGACAGTGATCGTTCACAACCGATGCTGACACAACGGTGGGGAAAAATACAAAAGGTGGTGAACAAATTTTCAGAATGTTTTGCAGCTATAAATGAAAGACATGAAAGTGGGAAGACTGAACAAGACAAGATTGCAGATGCAAAGAAGATGTTTGCAGCACAAGAAAAGTTTCGGTTCAATCTTGATCATGCTTGGATTTTGTTAAGGCACCAACCAAAGTGGATACAATTAATGCAAGAGCTGGACAGCAAGAAAAGTAAGCAAAGATCTAAATCTTGTATAACTAATATCTCATCATCCTCAACTACAGGCACTCACATAGATATTGAAGAAGACACGAACACTTGCTTTCCAGTGTCTAGACCACCGGGTAAGAAGGCTGAGAAAAGAAAGCTGAAAGAAACCACTGATCAATTGGTGCAAATAGAAAAATTACATCAAGAGAAAAAAGAAAGAGATGAAAAACAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

433

Amino Acids

50.25

Weight (kDa)

7.99

Isoelectric Point (pI)

53.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anoctamin PF04547 119 - 172 4.4e-13 Calcium-activated chloride channel
NAM-associated PF14303 335 - 432 2.1e-12 No apical meristem-associated C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 17, 722
AasI GACNNNNNNGTC 1 cut(s) 332
Acc36I ACCTGC 1 cut(s) 502
AccB1I GGYRCC 1 cut(s) 1035
AccI GTMKAC 1 cut(s) 336
AclI AACGTT 1 cut(s) 46
AcsI RAATTY 3 cut(s) 183, 780, 893
AfaI GTAC 5 cut(s) 504, 542, 595, 806, 818
AflIII ACRYGT 1 cut(s) 543
AgsI TTSAA 7 cut(s) 38, 77, 373, 736, 753, 1006, 1157
AhlI ACTAGT 2 cut(s) 596, 740
AleI CACNNNNGTG 1 cut(s) 862
Alw21I GWGCWC 2 cut(s) 234, 551
Alw26I GTCTC 1 cut(s) 388
AlwNI CAGNNNCTG 1 cut(s) 536
ApeKI GCWGC 5 cut(s) 376, 587, 775, 911, 983
ApoI RAATTY 3 cut(s) 183, 780, 893
AseI ATTAAT 1 cut(s) 1058
Asp700I GAANNNNTTC 1 cut(s) 996
AspLEI GCGC 1 cut(s) 482
AspS9I GGNCC 1 cut(s) 686
AsuC2I CCSGG 1 cut(s) 1197
AsuHPI GGTGA 1 cut(s) 898
AvaII GGWCC 1 cut(s) 686
BanI GGYRCC 1 cut(s) 1035
BauI CACGAG 1 cut(s) 405
BbsI GAAGAC 2 cut(s) 947, 1167
Bbv12I GWGCWC 2 cut(s) 234, 551
BbvI GCAGC 5 cut(s) 363, 599, 762, 923, 995
BciVI GTATCC 1 cut(s) 1044
BclI TGATCA 3 cut(s) 766, 1012, 1237
BcnI CCSGG 1 cut(s) 1197
BcoDI GTCTC 1 cut(s) 388
BcuI ACTAGT 2 cut(s) 596, 740
BfaI CTAG 8 cut(s) 83, 224, 254, 488, 597, 741, 1188, 1300
BfmI CTRYAG 1 cut(s) 1134
BfuAI ACCTGC 1 cut(s) 502
BfuI GTATCC 1 cut(s) 1044
BglII AGATCT 3 cut(s) 220, 270, 1094
BisI GCNGC 5 cut(s) 377, 588, 776, 912, 984
BlsI GCNGC 5 cut(s) 378, 589, 777, 913, 985
BmcAI AGTACT 1 cut(s) 806
Bme1390I CCNGG 1 cut(s) 1197
Bme18I GGWCC 1 cut(s) 686
BmgT120I GGNCC 1 cut(s) 686
BmiI GGNNCC 2 cut(s) 688, 1037
BmrFI CCNGG 1 cut(s) 1197
BmrI ACTGGG 1 cut(s) 603
BmsI GCATC 4 cut(s) 355, 375, 840, 955
BmuI ACTGGG 1 cut(s) 603
BpiI GAAGAC 2 cut(s) 947, 1167
BpuEI CTTGAG 1 cut(s) 606
BpuMI CCSGG 1 cut(s) 1197
BsaAI YACGTR 1 cut(s) 137
BsaBI GATNNNNATC 1 cut(s) 1099
BsaI GGTCTC 1 cut(s) 388
BsaXI ACNNNNNCTCC 2 cut(s) 533, 563
Bse1I ACTGG 2 cut(s) 609, 1181
Bse3DI GCAATG 3 cut(s) 57, 248, 769
Bse8I GATNNNNATC 1 cut(s) 1099
BseGI GGATG 2 cut(s) 461, 1124
BseJI GATNNNNATC 1 cut(s) 1099
BseMI GCAATG 3 cut(s) 57, 248, 769
BseMII CTCAG 3 cut(s) 528, 642, 1200
BseNI ACTGG 2 cut(s) 609, 1181
BseRI GAGGAG 1 cut(s) 719
BseXI GCAGC 5 cut(s) 363, 599, 762, 923, 995
BsgI GTGCAG 1 cut(s) 621
BshNI GGYRCC 1 cut(s) 1035
BsiHKAI GWGCWC 2 cut(s) 234, 551
BsiSI CCGG 1 cut(s) 1196
BslFI GGGAC 1 cut(s) 672
BsmAI GTCTC 1 cut(s) 388
BsmFI GGGAC 1 cut(s) 672
Bso31I GGTCTC 1 cut(s) 388
Bsp1286I GDGCHC 2 cut(s) 234, 551
Bsp143I GATC 8 cut(s) 70, 220, 270, 766, 835, 1012, 1094, 1237
BspCNI CTCAG 3 cut(s) 529, 641, 1201
BspLI GGNNCC 2 cut(s) 688, 1037
BspMI ACCTGC 1 cut(s) 502
BspT107I GGYRCC 1 cut(s) 1035
BspTNI GGTCTC 1 cut(s) 388
BsrDI GCAATG 3 cut(s) 57, 248, 769
BsrI ACTGG 2 cut(s) 609, 1181
BssMI GATC 8 cut(s) 70, 220, 270, 766, 835, 1012, 1094, 1237
BssSI CACGAG 1 cut(s) 405
Bst2BI CACGAG 1 cut(s) 405
Bst4CI ACNGT 3 cut(s) 515, 833, 864
BstAPI GCANNNNNTGC 1 cut(s) 477
BstBAI YACGTR 1 cut(s) 137
BstDEI CTNAG 3 cut(s) 537, 628, 1209
BstF5I GGATG 2 cut(s) 461, 1124
BstHHI GCGC 1 cut(s) 482
BstKTI GATC 8 cut(s) 73, 223, 273, 769, 838, 1015, 1097, 1240
BstMAI GTCTC 1 cut(s) 388
BstMBI GATC 8 cut(s) 70, 220, 270, 766, 835, 1012, 1094, 1237
BstMWI GCNNNNNNNGC 4 cut(s) 238, 385, 477, 533
BstNSI RCATGY 2 cut(s) 12, 547
BstSCI CCNGG 1 cut(s) 1195
BstSFI CTRYAG 1 cut(s) 1134
BstV1I GCAGC 5 cut(s) 363, 599, 762, 923, 995
BstV2I GAAGAC 2 cut(s) 947, 1167
BstX2I RGATCY 3 cut(s) 220, 270, 1094
BstYI RGATCY 3 cut(s) 220, 270, 1094
BsuI GTATCC 1 cut(s) 1044
BtsCI GGATG 2 cut(s) 461, 1124
BtsIMutI CAGTG 3 cut(s) 838, 1188, 1233
BveI ACCTGC 1 cut(s) 502
CaiI CAGNNNCTG 1 cut(s) 536
CfoI GCGC 1 cut(s) 482
Cfr13I GGNCC 1 cut(s) 686
Csp6I GTAC 5 cut(s) 503, 541, 594, 805, 817
CspCI CAANNNNNGTGG 4 cut(s) 403, 438, 1028, 1063
CviAII CATG 6 cut(s) 9, 28, 544, 635, 929, 1016
CviQI GTAC 5 cut(s) 503, 541, 594, 805, 817
DdeI CTNAG 3 cut(s) 537, 628, 1209
DpnI GATC 8 cut(s) 72, 222, 272, 768, 837, 1014, 1096, 1239
DpnII GATC 8 cut(s) 70, 220, 270, 766, 835, 1012, 1094, 1237
DrdI GACNNNNNNGTC 1 cut(s) 332
DseDI GACNNNNNNGTC 1 cut(s) 332
Eco31I GGTCTC 1 cut(s) 388
Eco47I GGWCC 1 cut(s) 686
EcoO109I RGGNCCY 1 cut(s) 686
EcoRI GAATTC 1 cut(s) 183
EcoT22I ATGCAT 2 cut(s) 370, 390
FaeI CATG 6 cut(s) 12, 31, 547, 638, 932, 1019
FalI AAGNNNNNCTT 2 cut(s) 57, 89
FaqI GGGAC 1 cut(s) 672
FatI CATG 6 cut(s) 8, 27, 543, 634, 928, 1015
FauNDI CATATG 2 cut(s) 236, 467
FbaI TGATCA 3 cut(s) 766, 1012, 1237
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 5 cut(s) 377, 588, 776, 912, 984
FokI GGATG 2 cut(s) 448, 1111
Fsp4HI GCNGC 5 cut(s) 377, 588, 776, 912, 984
FspBI CTAG 8 cut(s) 83, 224, 254, 488, 597, 741, 1188, 1300
GlaI GCGC 1 cut(s) 481
GluI GCNGC 5 cut(s) 377, 588, 776, 912, 984
HapII CCGG 1 cut(s) 1196
HhaI GCGC 1 cut(s) 482
Hin1II CATG 6 cut(s) 12, 31, 547, 638, 932, 1019
Hin6I GCGC 1 cut(s) 480
HinP1I GCGC 1 cut(s) 480
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 4 cut(s) 257, 626, 676, 749
HpaII CCGG 1 cut(s) 1196
HphI GGTGA 1 cut(s) 898
Hpy166II GTNNAC 3 cut(s) 337, 842, 889
Hpy188I TCNGA 3 cut(s) 52, 631, 901
Hpy188III TCNNGA 8 cut(s) 74, 188, 488, 623, 689, 1010, 1188, 1268
Hpy8I GTNNAC 3 cut(s) 337, 842, 889
HpyAV CCTTC 2 cut(s) 494, 1198
HpyCH4III ACNGT 3 cut(s) 515, 833, 864
HpyCH4IV ACGT 2 cut(s) 46, 136
HpyF10VI GCNNNNNNNGC 4 cut(s) 238, 385, 477, 533
HpyF3I CTNAG 3 cut(s) 537, 628, 1209
HpySE526I ACGT 2 cut(s) 46, 136
Hsp92II CATG 6 cut(s) 12, 31, 547, 638, 932, 1019
HspAI GCGC 1 cut(s) 480
Ksp22I TGATCA 3 cut(s) 766, 1012, 1237
Kzo9I GATC 8 cut(s) 70, 220, 270, 766, 835, 1012, 1094, 1237
LmnI GCTCC 2 cut(s) 216, 554
LpnPI CCDG 9 cut(s) 117, 246, 507, 576, 622, 1055, 1122, 1194, 1209
Lsp1109I GCAGC 5 cut(s) 363, 599, 762, 923, 995
LweI GCATC 4 cut(s) 355, 375, 840, 955
MaeI CTAG 8 cut(s) 83, 224, 254, 488, 597, 741, 1188, 1300
MaeII ACGT 2 cut(s) 46, 136
MaeIII GTNAC 2 cut(s) 188, 457
MalI GATC 8 cut(s) 72, 222, 272, 768, 837, 1014, 1096, 1239
MboI GATC 8 cut(s) 70, 220, 270, 766, 835, 1012, 1094, 1237
MboII GAAGA 5 cut(s) 50, 952, 985, 1169, 1172
MfeI CAATTG 2 cut(s) 420, 1241
MflI RGATCY 3 cut(s) 220, 270, 1094
MhlI GDGCHC 2 cut(s) 234, 551
MlyI GAGTC 2 cut(s) 620, 670
Mph1103I ATGCAT 2 cut(s) 370, 390
MroXI GAANNNNTTC 1 cut(s) 996
MseI TTAA 5 cut(s) 279, 288, 696, 1031, 1058
MslI CAYNNNNRTG 4 cut(s) 466, 639, 862, 933
MspA1I CMGCKG 1 cut(s) 590
MspI CCGG 1 cut(s) 1196
MspR9I CCNGG 1 cut(s) 1197
MunI CAATTG 2 cut(s) 420, 1241
MwoI GCNNNNNNNGC 4 cut(s) 238, 385, 477, 533
NciI CCSGG 1 cut(s) 1197
NdeI CATATG 2 cut(s) 236, 467
NdeII GATC 8 cut(s) 70, 220, 270, 766, 835, 1012, 1094, 1237
NlaIII CATG 6 cut(s) 12, 31, 547, 638, 932, 1019
NlaIV GGNNCC 2 cut(s) 688, 1037
NmuCI GTSAC 1 cut(s) 188
NsiI ATGCAT 2 cut(s) 370, 390
NspI RCATGY 2 cut(s) 12, 547
OliI CACNNNNGTG 1 cut(s) 862
PciI ACATGT 1 cut(s) 543
PcsI WCGNNNNNNNCGW 1 cut(s) 511
PdmI GAANNNNTTC 1 cut(s) 996
PfeI GAWTC 2 cut(s) 257, 749
PkrI GCNGC 5 cut(s) 378, 589, 777, 913, 985
PleI GAGTC 2 cut(s) 620, 670
PpsI GAGTC 2 cut(s) 620, 670
Ppu21I YACGTR 1 cut(s) 137
PpuMI RGGWCCY 1 cut(s) 686
PscI ACATGT 1 cut(s) 543
PshBI ATTAAT 1 cut(s) 1058
PsiI TTATAA 2 cut(s) 17, 722
Psp1406I AACGTT 1 cut(s) 46
Psp5II RGGWCCY 1 cut(s) 686
PspN4I GGNNCC 2 cut(s) 688, 1037
PspPI GGNCC 1 cut(s) 686
PspPPI RGGWCCY 1 cut(s) 686
PstNI CAGNNNCTG 1 cut(s) 536
PsuI RGATCY 3 cut(s) 220, 270, 1094
PvuII CAGCTG 1 cut(s) 590
RsaI GTAC 5 cut(s) 504, 542, 595, 806, 818
RsaNI GTAC 5 cut(s) 503, 541, 594, 805, 817
RseI CAYNNNNRTG 4 cut(s) 466, 639, 862, 933
SaqAI TTAA 5 cut(s) 279, 288, 696, 1031, 1058
SatI GCNGC 5 cut(s) 377, 588, 776, 912, 984
Sau3AI GATC 8 cut(s) 70, 220, 270, 766, 835, 1012, 1094, 1237
Sau96I GGNCC 1 cut(s) 686
ScaI AGTACT 1 cut(s) 806
SchI GAGTC 2 cut(s) 620, 670
ScrFI CCNGG 1 cut(s) 1197
SduI GDGCHC 2 cut(s) 234, 551
SfaNI GCATC 4 cut(s) 355, 375, 840, 955
SfcI CTRYAG 1 cut(s) 1134
SinI GGWCC 1 cut(s) 686
SmiMI CAYNNNNRTG 4 cut(s) 466, 639, 862, 933
SmlI CTYRAG 1 cut(s) 621
SmoI CTYRAG 1 cut(s) 621
SpeI ACTAGT 2 cut(s) 596, 740
SspI AATATT 1 cut(s) 571
SspMI CTAG 8 cut(s) 83, 224, 254, 488, 597, 741, 1188, 1300
StyD4I CCNGG 1 cut(s) 1195
TaaI ACNGT 3 cut(s) 515, 833, 864
TaiI ACGT 2 cut(s) 49, 139
TatI WGTACW 3 cut(s) 540, 804, 816
TfiI GAWTC 2 cut(s) 257, 749
Tru1I TTAA 5 cut(s) 279, 288, 696, 1031, 1058
Tru9I TTAA 5 cut(s) 279, 288, 696, 1031, 1058
TscAI CASTG 3 cut(s) 838, 1188, 1240
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 5 cut(s) 376, 587, 775, 911, 983
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 7 cut(s) 17, 57, 318, 651, 936, 945, 1302
TspRI CASTG 3 cut(s) 838, 1188, 1240
VpaK11BI GGWCC 1 cut(s) 686
VspI ATTAAT 1 cut(s) 1058
XapI RAATTY 3 cut(s) 183, 780, 893
XbaI TCTAGA 2 cut(s) 487, 1187
XceI RCATGY 2 cut(s) 12, 547
XcmI CCANNNNNNNNNTGG 1 cut(s) 1240
XmiI GTMKAC 1 cut(s) 336
XmnI GAANNNNTTC 1 cut(s) 996
XspI CTAG 8 cut(s) 83, 224, 254, 488, 597, 741, 1188, 1300
ZrmI AGTACT 1 cut(s) 806
Zsp2I ATGCAT 2 cut(s) 370, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.