RLG00000024640

Tropinone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008147
Physical Location & Seq
Reverse (-)
37764930 .. 37766041
1112 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024640

Sequence Viewer

Length: 543 bp
ATGAGAACTGTTTCCTCAAATTTTGACGGCGAACTTAACATCCTTGTAAATAATGCAGCAACAGTCACTCTAAGAAACACTACAGAATATGATCTGGAAGATTTGTCAAGTATGATGAGCACAAATGTTGAATCCCCCTACCATCTTTGTCAACTTGCATATCCTCTCCTCAAGGCCTCCGGAAATGGAAGTATTGTATTCATCGCCTCCATTGCTGGTTTAAAAGCTCTACCTAGACTATCTGCCTATGCAGCAACGAAAAGTGCCGTCATCCAGATTTCAAAGAACTTGGCGTGTGAATGGGCTAAAGACGGCATTCGAACAAACGCTGTAGCACCTTGGGCTGTTAACACCGGAGTTAAAGTTGAAAACGATGACCACTCTGACGACTTCAGACGGCTAATAAGTCGAACTCCTATCCGTCGCGTTGCGGAACCCAATGAAATCTCATCATTGGTCACTTTTCTCTGCCTTCCTGCTGCTTCTTACATCAATGGACAAGTTGTTAGTGTTGATGGAGGATTTACCGTCAGCGGTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

181

Amino Acids

19.4

Weight (kDa)

5.66

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short_C2 PF13561 3 - 176 2.5e-43 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 3 - 125 1.8e-31 short chain dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 426
AccIII TCCGGA 1 cut(s) 179
AciI CCGC 2 cut(s) 431, 534
AcsI RAATTY 1 cut(s) 19
AcuI CTGAAG 1 cut(s) 376
AgsI TTSAA 3 cut(s) 131, 282, 368
AloI GAACNNNNNNTCC 2 cut(s) 24, 56
AluBI AGCT 1 cut(s) 227
AluI AGCT 1 cut(s) 227
Alw21I GWGCWC 1 cut(s) 122
Aor13HI TCCGGA 1 cut(s) 179
AoxI GGCC 1 cut(s) 174
ApeKI GCWGC 3 cut(s) 56, 251, 479
ApoI RAATTY 1 cut(s) 19
Asp700I GAANNNNTTC 1 cut(s) 10
AsuII TTCGAA 1 cut(s) 319
Bbv12I GWGCWC 1 cut(s) 122
BbvI GCAGC 3 cut(s) 68, 263, 466
BccI CCATC 2 cut(s) 150, 509
BceAI ACGGC 4 cut(s) 43, 251, 328, 413
BfaI CTAG 1 cut(s) 234
BfmI CTRYAG 2 cut(s) 81, 330
BisI GCNGC 3 cut(s) 57, 252, 480
BlsI GCNGC 3 cut(s) 58, 253, 481
BmiI GGNNCC 1 cut(s) 435
Bpu14I TTCGAA 1 cut(s) 319
BpuEI CTTGAG 1 cut(s) 155
BsaJI CCNNGG 1 cut(s) 338
BsaWI WCCGGW 2 cut(s) 179, 353
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bse3DI GCAATG 1 cut(s) 210
BseAI TCCGGA 1 cut(s) 179
BseDI CCNNGG 1 cut(s) 338
BseGI GGATG 2 cut(s) 39, 270
BseMI GCAATG 1 cut(s) 210
BseRI GAGGAG 1 cut(s) 158
BseXI GCAGC 3 cut(s) 68, 263, 466
Bsh1236I CGCG 1 cut(s) 426
BshFI GGCC 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 122
BsiSI CCGG 2 cut(s) 180, 354
BsmI GAATGC 1 cut(s) 315
BsnI GGCC 1 cut(s) 176
Bsp119I TTCGAA 1 cut(s) 319
Bsp1286I GDGCHC 1 cut(s) 122
Bsp13I TCCGGA 1 cut(s) 179
Bsp143I GATC 1 cut(s) 91
BspACI CCGC 2 cut(s) 431, 534
BspANI GGCC 1 cut(s) 176
BspEI TCCGGA 1 cut(s) 179
BspFNI CGCG 1 cut(s) 426
BspLI GGNNCC 1 cut(s) 435
BspT104I TTCGAA 1 cut(s) 319
BsrDI GCAATG 1 cut(s) 210
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 1 cut(s) 91
BssT1I CCWWGG 1 cut(s) 338
Bst4CI ACNGT 3 cut(s) 10, 64, 529
BstBI TTCGAA 1 cut(s) 319
BstDEI CTNAG 1 cut(s) 71
BstF5I GGATG 2 cut(s) 39, 270
BstFNI CGCG 1 cut(s) 426
BstKTI GATC 1 cut(s) 94
BstMBI GATC 1 cut(s) 91
BstMWI GCNNNNNNNGC 3 cut(s) 212, 251, 341
BstSFI CTRYAG 2 cut(s) 81, 330
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 3 cut(s) 68, 263, 466
BsuRI GGCC 1 cut(s) 176
BtgZI GCGATG 1 cut(s) 187
BtsCI GGATG 2 cut(s) 39, 270
CviJI RGCY 5 cut(s) 176, 227, 305, 344, 400
CviKI_1 RGCY 5 cut(s) 176, 227, 305, 344, 400
DdeI CTNAG 1 cut(s) 71
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
DraI TTTAAA 1 cut(s) 222
Eco130I CCWWGG 1 cut(s) 338
Eco147I AGGCCT 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 376
EcoT14I CCWWGG 1 cut(s) 338
ErhI CCWWGG 1 cut(s) 338
FaiI YATR 4 cut(s) 90, 113, 160, 249
Fnu4HI GCNGC 3 cut(s) 57, 252, 480
FokI GGATG 2 cut(s) 26, 257
Fsp4HI GCNGC 3 cut(s) 57, 252, 480
FspBI CTAG 1 cut(s) 234
GluI GCNGC 3 cut(s) 57, 252, 480
HaeIII GGCC 1 cut(s) 176
HapII CCGG 2 cut(s) 180, 354
HincII GTYRAC 2 cut(s) 152, 349
HindII GTYRAC 2 cut(s) 152, 349
HinfI GANTC 1 cut(s) 131
HpaI GTTAAC 1 cut(s) 349
HpaII CCGG 2 cut(s) 180, 354
Hpy166II GTNNAC 2 cut(s) 152, 349
Hpy188I TCNGA 2 cut(s) 385, 395
Hpy188III TCNNGA 3 cut(s) 95, 180, 274
Hpy8I GTNNAC 2 cut(s) 152, 349
Hpy99I CGWCG 1 cut(s) 426
HpyAV CCTTC 1 cut(s) 482
HpyCH4III ACNGT 3 cut(s) 10, 64, 529
HpyCH4V TGCA 3 cut(s) 56, 158, 251
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 251, 341
HpyF3I CTNAG 1 cut(s) 71
Kpn2I TCCGGA 1 cut(s) 179
KspAI GTTAAC 1 cut(s) 349
Kzo9I GATC 1 cut(s) 91
LpnPI CCDG 6 cut(s) 80, 193, 201, 287, 367, 489
Lsp1109I GCAGC 3 cut(s) 68, 263, 466
MaeI CTAG 1 cut(s) 234
MaeIII GTNAC 2 cut(s) 64, 457
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MboII GAAGA 1 cut(s) 110
MhlI GDGCHC 1 cut(s) 122
MluCI AATT 1 cut(s) 19
MnlI CCTC 6 cut(s) 25, 174, 179, 187, 217, 512
MroI TCCGGA 1 cut(s) 179
MroXI GAANNNNTTC 1 cut(s) 10
MseI TTAA 4 cut(s) 36, 221, 348, 360
MspA1I CMGCKG 1 cut(s) 534
MspI CCGG 2 cut(s) 180, 354
Mva1269I GAATGC 1 cut(s) 315
MvnI CGCG 1 cut(s) 426
MwoI GCNNNNNNNGC 3 cut(s) 212, 251, 341
NdeII GATC 1 cut(s) 91
NlaIV GGNNCC 1 cut(s) 435
NmuCI GTSAC 2 cut(s) 64, 457
NspV TTCGAA 1 cut(s) 319
PceI AGGCCT 1 cut(s) 176
PctI GAATGC 1 cut(s) 315
PdmI GAANNNNTTC 1 cut(s) 10
PfeI GAWTC 1 cut(s) 131
PkrI GCNGC 3 cut(s) 58, 253, 481
PspN4I GGNNCC 1 cut(s) 435
SaqAI TTAA 4 cut(s) 36, 221, 348, 360
SatI GCNGC 3 cut(s) 57, 252, 480
Sau3AI GATC 1 cut(s) 91
SduI GDGCHC 1 cut(s) 122
SetI ASST 3 cut(s) 229, 235, 340
SfcI CTRYAG 2 cut(s) 81, 330
SfuI TTCGAA 1 cut(s) 319
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 1 cut(s) 19
SseBI AGGCCT 1 cut(s) 176
SsiI CCGC 2 cut(s) 431, 534
SspMI CTAG 1 cut(s) 234
StuI AGGCCT 1 cut(s) 176
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 3 cut(s) 10, 64, 529
TaqI TCGA 2 cut(s) 319, 409
TasI AATT 1 cut(s) 19
TfiI GAWTC 1 cut(s) 131
Tru1I TTAA 4 cut(s) 36, 221, 348, 360
Tru9I TTAA 4 cut(s) 36, 221, 348, 360
TseFI GTSAC 2 cut(s) 64, 457
TseI GCWGC 3 cut(s) 56, 251, 479
Tsp45I GTSAC 2 cut(s) 64, 457
TspDTI ATGAA 2 cut(s) 190, 456
TspGWI ACGGA 1 cut(s) 410
XapI RAATTY 1 cut(s) 19
XmnI GAANNNNTTC 1 cut(s) 10
XspI CTAG 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.