MD07G1214900.v1.1

Four repeated domains in the Fasciclin I family of proteins, present in many other contexts.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005494
Physical Location & Seq
Forward (+)
29227255 .. 29228004
750 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1214900.v1.1.491

Sequence Viewer

Length: 750 bp
ATGCTTTTAAAATTCTTACATCATCTCAGGCTTTTTCAGCCTTCGTCTCAACTTCTCGACTGCCGGAAACTGGAGTTGGACCTCAGCAGCAACTCCATCTCGGCTGAATCAAACGCCTTCCCTGCCGCCGCGTCCCCAGATCCCGACCGTCCTGAACCTCAGACGATGAGCAGAACCCGGATTCTCAGAAACCCGATTGTATTCGTATTCGGGTTCGTGTCGATTTCTTGTTTCTTAATCCTCACAATCTCAATCCTGCGGCTTCCAGACATCCCGATCGGACCATACGCCGCCACAAAAACCAGCCGAAAATCTTCACAAAACGACAAATTACCGATCGGGAAATTCGGAGAAATGACGCTCGAAATGCTGCCGGAAGATCTTCCCTTCACCGTGTTCCTCCCTTCGGAGAGAGCCTTCGCGCGCGATCTGCGGCTGAGATGGAAGGATAGCTTGTCGCCGGAGAAGATGAACGACACGTACGCCATAATTTCGCGAGTGCTGGGGTTCTCGACGGTGCCGAGGAGTATTGCTTCGGACAGTGTGCCGATTGGGAAGGAGCTGTCGTACGATTCGATATCTGGGTTTACGCTGTGGTTGTCAAAGGAGGAGGACGGGGCGGTGATGGTGAACGGAGTTCGGTCGGAGAGGGTTGATGTAAGGAGGAAGGCGAGCGTGGTGCATGTTATGGATGGGGTTGTCATGGATGCCGAGTTCCAGCAAGCGGTTCAGACTGAAGAAGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fasciclin

Protein Analysis

250

Amino Acids

27.89

Weight (kDa)

5.54

Isoelectric Point (pI)

63.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fasciclin PF02469 122 - 235 4.2e-08 Fasciclin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 517
AccII CGCG 5 cut(s) 131, 422, 424, 426, 496
AciI CCGC 7 cut(s) 126, 129, 259, 291, 433, 620, 725
AclWI GGATC 1 cut(s) 134
AcsI RAATTY 2 cut(s) 11, 344
AfaI GTAC 2 cut(s) 482, 569
AfiI CCNNNNNNNGG 3 cut(s) 70, 406, 724
AflIII ACRYGT 1 cut(s) 477
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AloI GAACNNNNNNTCC 2 cut(s) 698, 730
AluBI AGCT 2 cut(s) 453, 562
AluI AGCT 2 cut(s) 453, 562
Alw26I GTCTC 1 cut(s) 51
AlwI GGATC 1 cut(s) 134
ApeKI GCWGC 2 cut(s) 87, 370
ApoI RAATTY 2 cut(s) 11, 344
Asp700I GAANNNNTTC 2 cut(s) 313, 381
AspLEI GCGC 2 cut(s) 424, 426
AspS9I GGNCC 2 cut(s) 79, 281
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 3 cut(s) 382, 634, 640
AvaII GGWCC 2 cut(s) 79, 281
BanI GGYRCC 1 cut(s) 517
BbvCI CCTCAGC 1 cut(s) 83
BbvI GCAGC 2 cut(s) 99, 357
BccI CCATC 4 cut(s) 104, 435, 619, 686
BcgI CGANNNNNNTGC 2 cut(s) 661, 695
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 1 cut(s) 51
BglII AGATCT 1 cut(s) 379
BisI GCNGC 7 cut(s) 88, 126, 129, 260, 291, 371, 434
BlsI GCNGC 7 cut(s) 89, 127, 130, 261, 292, 372, 435
Bme1390I CCNGG 1 cut(s) 178
Bme18I GGWCC 2 cut(s) 79, 281
BmgT120I GGNCC 2 cut(s) 79, 281
BmiI GGNNCC 1 cut(s) 519
BmrFI CCNGG 1 cut(s) 178
BmsI GCATC 1 cut(s) 697
BpmI CTGGAG 1 cut(s) 92
Bpu10I CCTNAGC 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 178
BsaAI YACGTR 1 cut(s) 480
BsaJI CCNNGG 1 cut(s) 521
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 406, 724
Bse1I ACTGG 1 cut(s) 75
BseDI CCNNGG 1 cut(s) 521
BseGI GGATG 3 cut(s) 270, 697, 712
BseLI CCNNNNNNNGG 3 cut(s) 70, 406, 724
BseMII CTCAG 5 cut(s) 40, 97, 173, 199, 428
BseNI ACTGG 1 cut(s) 75
BsePI GCGCGC 1 cut(s) 422
BseRI GAGGAG 2 cut(s) 538, 623
BseXI GCAGC 2 cut(s) 99, 357
BseYI CCCAGC 1 cut(s) 502
Bsh1236I CGCG 5 cut(s) 131, 422, 424, 426, 496
Bsh1285I CGRYCG 4 cut(s) 148, 279, 339, 644
BshNI GGYRCC 1 cut(s) 517
BsiEI CGRYCG 4 cut(s) 148, 279, 339, 644
BsiSI CCGG 4 cut(s) 64, 178, 374, 461
BsiWI CGTACG 2 cut(s) 480, 567
BslFI GGGAC 1 cut(s) 118
BslI CCNNNNNNNGG 3 cut(s) 70, 406, 724
BsmAI GTCTC 1 cut(s) 51
BsmBI CGTCTC 1 cut(s) 51
BsmFI GGGAC 1 cut(s) 118
Bsp143I GATC 5 cut(s) 139, 276, 336, 379, 427
Bsp68I TCGCGA 1 cut(s) 496
BspACI CCGC 7 cut(s) 126, 129, 259, 291, 433, 620, 725
BspCNI CTCAG 5 cut(s) 39, 96, 172, 198, 429
BspFNI CGCG 5 cut(s) 131, 422, 424, 426, 496
BspLI GGNNCC 1 cut(s) 519
BspPI GGATC 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 517
BsrI ACTGG 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 521
BssHII GCGCGC 1 cut(s) 422
BssMI GATC 5 cut(s) 139, 276, 336, 379, 427
Bst4CI ACNGT 4 cut(s) 149, 394, 517, 542
BstBAI YACGTR 1 cut(s) 480
BstC8I GCNNGC 3 cut(s) 424, 673, 723
BstDEI CTNAG 5 cut(s) 26, 83, 159, 185, 437
BstF5I GGATG 3 cut(s) 270, 697, 712
BstFNI CGCG 5 cut(s) 131, 422, 424, 426, 496
BstHHI GCGC 2 cut(s) 424, 426
BstKTI GATC 5 cut(s) 142, 279, 339, 382, 430
BstMAI GTCTC 1 cut(s) 51
BstMBI GATC 5 cut(s) 139, 276, 336, 379, 427
BstMCI CGRYCG 4 cut(s) 148, 279, 339, 644
BstMWI GCNNNNNNNGC 4 cut(s) 37, 122, 367, 430
BstNSI RCATGY 1 cut(s) 686
BstSCI CCNGG 1 cut(s) 176
BstUI CGCG 5 cut(s) 131, 422, 424, 426, 496
BstV1I GCAGC 2 cut(s) 99, 357
BstX2I RGATCY 2 cut(s) 139, 379
BstYI RGATCY 2 cut(s) 139, 379
BtsCI GGATG 3 cut(s) 270, 697, 712
BtsIMutI CAGTG 1 cut(s) 547
BtuMI TCGCGA 1 cut(s) 496
Cac8I GCNNGC 3 cut(s) 424, 673, 723
CfoI GCGC 2 cut(s) 424, 426
Cfr13I GGNCC 2 cut(s) 79, 281
CseI GACGC 2 cut(s) 120, 367
Csp6I GTAC 2 cut(s) 481, 568
CviAII CATG 2 cut(s) 683, 703
CviJI RGCY 9 cut(s) 31, 40, 104, 262, 306, 416, 436, 453, 562
CviKI_1 RGCY 9 cut(s) 31, 40, 104, 262, 306, 416, 436, 453, 562
CviQI GTAC 2 cut(s) 481, 568
DdeI CTNAG 5 cut(s) 26, 83, 159, 185, 437
DpnI GATC 5 cut(s) 141, 278, 338, 381, 429
DpnII GATC 5 cut(s) 139, 276, 336, 379, 427
DraI TTTAAA 1 cut(s) 9
Eco32I GATATC 1 cut(s) 579
Eco47I GGWCC 2 cut(s) 79, 281
EcoRV GATATC 1 cut(s) 579
Esp3I CGTCTC 1 cut(s) 51
FaeI CATG 2 cut(s) 686, 706
FaiI YATR 5 cut(s) 286, 488, 684, 689, 704
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FaqI GGGAC 1 cut(s) 118
FatI CATG 2 cut(s) 682, 702
Fnu4HI GCNGC 7 cut(s) 88, 126, 129, 260, 291, 371, 434
FokI GGATG 3 cut(s) 257, 704, 719
Fsp4HI GCNGC 7 cut(s) 88, 126, 129, 260, 291, 371, 434
GlaI GCGC 2 cut(s) 423, 425
GluI GCNGC 7 cut(s) 88, 126, 129, 260, 291, 371, 434
GsaI CCCAGC 1 cut(s) 506
GsuI CTGGAG 1 cut(s) 92
HapII CCGG 4 cut(s) 64, 178, 374, 461
HgaI GACGC 2 cut(s) 120, 367
HhaI GCGC 2 cut(s) 424, 426
Hin1II CATG 2 cut(s) 686, 706
Hin6I GCGC 2 cut(s) 422, 424
HinP1I GCGC 2 cut(s) 422, 424
HinfI GANTC 3 cut(s) 107, 181, 572
HpaII CCGG 4 cut(s) 64, 178, 374, 461
HphI GGTGA 3 cut(s) 382, 634, 640
Hpy166II GTNNAC 2 cut(s) 588, 631
Hpy188I TCNGA 8 cut(s) 162, 188, 281, 350, 409, 538, 646, 732
Hpy188III TCNNGA 8 cut(s) 56, 143, 152, 266, 274, 340, 495, 511
Hpy8I GTNNAC 2 cut(s) 588, 631
Hpy99I CGWCG 1 cut(s) 517
HpyAV CCTTC 8 cut(s) 51, 127, 397, 414, 427, 439, 550, 661
HpyCH4III ACNGT 4 cut(s) 149, 394, 517, 542
HpyCH4IV ACGT 1 cut(s) 479
HpyCH4V TGCA 1 cut(s) 682
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 122, 367, 430
HpyF3I CTNAG 5 cut(s) 26, 83, 159, 185, 437
HpySE526I ACGT 1 cut(s) 479
Hsp92II CATG 2 cut(s) 686, 706
HspAI GCGC 2 cut(s) 422, 424
Kzo9I GATC 5 cut(s) 139, 276, 336, 379, 427
LmnI GCTCC 1 cut(s) 559
Lsp1109I GCAGC 2 cut(s) 99, 357
LweI GCATC 1 cut(s) 697
MaeII ACGT 1 cut(s) 479
MalI GATC 5 cut(s) 141, 278, 338, 381, 429
MauBI CGCGCGCG 1 cut(s) 422
MboI GATC 5 cut(s) 139, 276, 336, 379, 427
MboII GAAGA 5 cut(s) 306, 374, 389, 478, 749
MflI RGATCY 2 cut(s) 139, 379
MluCI AATT 4 cut(s) 11, 329, 344, 489
MmeI TCCRAC 2 cut(s) 57, 624
MnlI CCTC 9 cut(s) 92, 168, 251, 410, 516, 601, 604, 642, 657
MroXI GAANNNNTTC 2 cut(s) 313, 381
MseI TTAA 2 cut(s) 8, 236
MspI CCGG 4 cut(s) 64, 178, 374, 461
MspR9I CCNGG 1 cut(s) 178
MvnI CGCG 5 cut(s) 131, 422, 424, 426, 496
MwoI GCNNNNNNNGC 4 cut(s) 37, 122, 367, 430
NciI CCSGG 1 cut(s) 178
NdeII GATC 5 cut(s) 139, 276, 336, 379, 427
NlaIII CATG 2 cut(s) 686, 706
NlaIV GGNNCC 1 cut(s) 519
NmeAIII GCCGAG 3 cut(s) 80, 546, 736
NruI TCGCGA 1 cut(s) 496
NspI RCATGY 1 cut(s) 686
PauI GCGCGC 1 cut(s) 422
PcsI WCGNNNNNNNCGW 2 cut(s) 518, 572
PdmI GAANNNNTTC 2 cut(s) 313, 381
PfeI GAWTC 3 cut(s) 107, 181, 572
Pfl23II CGTACG 2 cut(s) 480, 567
PkrI GCNGC 7 cut(s) 89, 127, 130, 261, 292, 372, 435
Ple19I CGATCG 2 cut(s) 279, 339
Ppu21I YACGTR 1 cut(s) 480
PspFI CCCAGC 1 cut(s) 502
PspLI CGTACG 2 cut(s) 480, 567
PspN4I GGNNCC 1 cut(s) 519
PspPI GGNCC 2 cut(s) 79, 281
PsrI GAACNNNNNNTAC 2 cut(s) 464, 496
PsuI RGATCY 2 cut(s) 139, 379
PteI GCGCGC 1 cut(s) 422
PvuI CGATCG 2 cut(s) 279, 339
RruI TCGCGA 1 cut(s) 496
RsaI GTAC 2 cut(s) 482, 569
RsaNI GTAC 2 cut(s) 481, 568
SaqAI TTAA 2 cut(s) 8, 236
SatI GCNGC 7 cut(s) 88, 126, 129, 260, 291, 371, 434
Sau3AI GATC 5 cut(s) 139, 276, 336, 379, 427
Sau96I GGNCC 2 cut(s) 79, 281
ScrFI CCNGG 1 cut(s) 178
SetI ASST 5 cut(s) 84, 160, 455, 482, 564
SfaNI GCATC 1 cut(s) 697
SinI GGWCC 2 cut(s) 79, 281
Sse9I AATT 4 cut(s) 11, 329, 344, 489
SsiI CCGC 7 cut(s) 126, 129, 259, 291, 433, 620, 725
StyD4I CCNGG 1 cut(s) 176
TaaI ACNGT 4 cut(s) 149, 394, 517, 542
TaiI ACGT 1 cut(s) 482
TaqI TCGA 5 cut(s) 57, 221, 363, 512, 575
TaqII GACCGA 1 cut(s) 630
TasI AATT 4 cut(s) 11, 329, 344, 489
TauI GCSGC 5 cut(s) 128, 131, 262, 293, 436
TfiI GAWTC 3 cut(s) 107, 181, 572
Tru1I TTAA 2 cut(s) 8, 236
Tru9I TTAA 2 cut(s) 8, 236
TscAI CASTG 1 cut(s) 547
TseI GCWGC 2 cut(s) 87, 370
TspDTI ATGAA 1 cut(s) 485
TspGWI ACGGA 1 cut(s) 648
TspRI CASTG 1 cut(s) 547
VpaK11BI GGWCC 2 cut(s) 79, 281
XapI RAATTY 2 cut(s) 11, 344
XceI RCATGY 1 cut(s) 686
XmnI GAANNNNTTC 2 cut(s) 313, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.