Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0010326

Physical Location & Seq

Genomic Coordinates Forward (+)

29894544 .. 29898885

Gene Structure 4342 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1222000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2493 bp

ATGCCCTCAAACCACTCGGACTCCGACACCGACTCCGACCAGAGCCAATCTGGCTCCGGACCCGTTCGCCACTCGGATCTCTCCGACTCCATTTTCCGAACCTACTTGGAATTCACAGGAAAATCAACAGCGACTACGGCCGACCTCTCCAAGATCCAATCTTTCCTGACGTCATCCTCCTCCGGCGCTCTGTCATGCCTGATATGCCTGGAGCGGATCCGGCCCGCCGACCCGACATGGTCCTGCACCTCCGTCTGTTTCTCCGTCTTCCACCTCATATGCATCCAGAGCTGGGCGCGCCAGGCCTCCGATTTGTCCGCCTTGCGGGCCTCCACCCGCCTTCCCATTTCCGCCCAGAAAGCCGCCGAGGTATCAGTCTGGAACTGCCCCAAATGTAGGATAGAGTACCCCCATTCTCAAATCCCCAAGAATTATTACTGTTTCTGTGGAAAGCTCGAGAACCCACCGAGCGATGACCCGTGGGTTCTGCCGCATTCGTGCGGCGAGGTCTGCAACCGGCCGCTGAAGCACGGCTGTGGCCACCATTGCTTGCTTCTTTGCCACCCGGGTCCCTGCCCGTCCTGCCCGAAGCTCGTAGAATCTCGGTGCTTTTGTGGGTCTGTGAAGGACGTCCGGCGATGTGGGTTTAAGTATTTCTCTTGTAATAATGCTTGTAAGAAGGTATTGGCTTGTGGGATCCACCGGTGCTCGGAGATTTGCCACCAGGGGCCCTGCTTGCCGTGCCGGGTGCGGGGAGTGTACCGGTGCCAATGTGGGAAGAAGGAGGAGGAGAAGGAGTGTTGTGAGCGGCTTGATTTTAGGTGTGAGGAGCCGTGCGAGAAGGTGCTTGGCTGTGGGCAGCATAAGTGTACCAAAGGGTGTCATTTGGGGGAGTGCGGGCCGTGCCCGTCCCAGGGGAAGAGGACATGCCCCTGTGGAAAGAGAATGCATGAGGGATTGTCTTGCAAAGCCTCTGTGCCTTTGTGTGGTTCAACTTGTGATAAGATGCTCAGTTGCGGCTACCATAGGTGCCCGGAGAGGTGTCATCGTGGGGAGTGCATTGAGACTTGCAGAACAGTTGTCATTAAGTATTGCCGGTGCGGGAGCTTGAAAAAGGAGGTTCCTTGCCATCAAGATTTAGCATGCGAAAGGAAGTGCCAGAGACTAAGGGACTGTGGGCGGCATGCTTGTAAGCGCAGGTGCTGTGATGGGGACTGCCCACCGTGCTCAGAGATTTGTGGAAGAAAGCTACGATGTAGGAACCACAAGTGCCCATCTCCATGCCATAGAGGTGCTTGTGCTCCGTGCCCAGTGATGGTGACAATTACATGTGCATGCGGTCAGACACACTTTGAGGTTCCTTGCGGTACTGAGATGGATCAAAAGCCTCCCAGATGTCGTAAGCCATGCCCTATTACTCCTTTATGCAGGCATGGACCAAATTCCAAGCCACATAAATGCCATTATGGAGCCTGCCACCCGTGTAGATTACTTTGTGAGGAAGAATACCCATGTGGCCACAATTGCAAGCTAAGGTGTCATGGTCCTAAACCTCCTCCGAATCCGGAATTTACGTTGAAACCTAAGAAGAAGAAATCCTTTCATCAGAGTGAATCCACTCCAGGATCTCCATGCCCTCCATGCCCAGAACTTGTTTGGAGGTCATGTGTTGGCCAGCACATCGGGGCAGATAAAATGATAAAACACTATTTTTCCTGCGAAAACTTGTGCGGAAATCCTCTACCTTGTGGTAATCACTTTTGCACAAAAACTTGTCACCCCCTCAAGAATCAAACGGTGCCATCAGTCCGACAAGCAAGAAGTGAGCCTTGTGAGAAGTGTCAACTTCCTTGCAAAAAGGAGAGGGAGCCTGCATGTCCTCATCCTTGTCCTTTGCCATGTCATCCTGGAGACTGCCCTCCTTGCAAGGTGCTTGTAAAGCGATCGTGTCACTGTGGTTCCATGGTTCATCAAATGGCTGCTCGTTCATGTAAGGGGCCTTGTCACAGAAAGTTGCCGTATTGTACTCATCTGTGCCCAGAGATTTGTCATCCTGGTCAATGTTCATCACCCGAAAAGTGCTCTAAAAAGGTTACTGTCCGTTGTGGATGCCAGAGCTTGAAAAAGGAGTGGTTATGTCACAATGTTCAGGCAGCCTATCGCGATGCTGGTTCTGATCCCAAAGATGTATTTAAGAATCAATTTGGACTTGGACTCATTCCTTGCAATTCCGACTGCAAAAGCAAACAAAAGGTTAATGATTCAGAATTACAGTTGCGTAGACCTAAATCTGTGGAGGTGAAGGAATCAGATAATGATAGGCAAGGACCAAAGCGGAAGAAAAGGCGTGAGCGGTTGCAACAAGTGACACAAACCTCGACACTGCAAGTATGTATTTCCTTGAAAGAAAAGACCGAGCACTTTGAACTGCCTCATTATTCACTTGCTAACTGTAACTCTTCATTTTCAAACAGAAAATTATTTCCAACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0000976 GO:0000977 GO:0000981 GO:0000982 GO:0001012 GO:0001067 GO:0001078 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009628 GO:0009651 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010310 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051193 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000377 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-NF-X1

Protein Analysis

831

Amino Acids

92.52

Weight (kDa)

8.84

Isoelectric Point (pI)

62.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-NF-X1	PF01422	231 - 249	5e-06	NF-X1 type zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010326)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05660
fragaria_vesca	FvH4_7g24480
malus_domestica	MD00G1166700.v1.1 MD07G1222000.v1.1
prunus_persica	Prupe.2G250200_v2.0.a1
pyrus_communis	pycom07g20050
rosa_chinensis	RchiOBHm_Chr1g0370241
rosa_laevigata	RLG00000027066
rosa_multiflora	Rmu_co8251429.1_g000001 Rmu_co8258023.1_g000001 Rmu_sc0018500.1_g000001
rosa_roxburghii	Rroxscaffold_4G00286720 Rroxscaffold_4G00287290
rosa_rugosa	Rorug01G0354900 Rorug01G0355000 Rorug01G0355100
rosa_samantha	Rh1AG363700 Rh1BG326800 Rh1CG340100 Rh1DG357200
rosa_wichuraiana	Rw1G032000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1188
AatII	GACGTC	2 cut(s)	173, 633
Acc36I	ACCTGC	1 cut(s)	1188
AccB1I	GGYRCC	3 cut(s)	765, 1029, 1798
AccBSI	CCGCTC	3 cut(s)	214, 808, 2353
AccI	GTMKAC	1 cut(s)	2281
AccII	CGCG	2 cut(s)	298, 2163
AccIII	TCCGGA	2 cut(s)	56, 1564
AclWI	GGATC	9 cut(s)	84, 148, 211, 224, 691, 704, 1386, 1633, 2171
AcoI	YGGCCR	5 cut(s)	138, 518, 538, 1516, 1672
AcsI	RAATTY	3 cut(s)	110, 1441, 1568
AcuI	CTGAAG	1 cut(s)	545
AcyI	GRCGYC	2 cut(s)	170, 630
AfaI	GTAC	5 cut(s)	407, 761, 871, 1369, 2026
AfiI	CCNNNNNNNGG	9 cut(s)	292, 324, 396, 709, 751, 913, 914, 986, 1315
AflIII	ACRYGT	1 cut(s)	1328
AgeI	ACCGGT	2 cut(s)	702, 762
AgsI	TTSAA	7 cut(s)	993, 1111, 1579, 2122, 2404, 2426, 2469
AjnI	CCWGG	7 cut(s)	207, 300, 723, 912, 1621, 1906, 2053
AleI	CACNNNNGTG	1 cut(s)	534
AluBI	AGCT	7 cut(s)	291, 454, 592, 1107, 1249, 1531, 2118
AluI	AGCT	7 cut(s)	291, 454, 592, 1107, 1249, 1531, 2118
Alw21I	GWGCWC	5 cut(s)	710, 1229, 1303, 2084, 2421
Alw26I	GTCTC	3 cut(s)	1058, 1156, 1905
AlwI	GGATC	9 cut(s)	84, 148, 211, 224, 691, 704, 1386, 1633, 2171
AlwNI	CAGNNNCTG	1 cut(s)	1203
Ama87I	CYCGRG	2 cut(s)	455, 565
Aor13HI	TCCGGA	2 cut(s)	56, 1564
ApaI	GGGCCC	1 cut(s)	732
ApeKI	GCWGC	3 cut(s)	859, 1979, 2153
ApoI	RAATTY	3 cut(s)	110, 1441, 1568
AscI	GGCGCGCC	1 cut(s)	296
AsiGI	ACCGGT	2 cut(s)	702, 762
AspLEI	GCGC	4 cut(s)	188, 298, 300, 1197
AsuC2I	CCSGG	4 cut(s)	566, 567, 746, 1034
AsuHPI	GGTGA	4 cut(s)	1330, 1769, 2061, 2311
AvaI	CYCGRG	2 cut(s)	455, 565
AvaII	GGWCC	6 cut(s)	59, 240, 569, 1436, 1544, 2327
BaeGI	GKGCMC	6 cut(s)	732, 908, 1034, 1274, 1310, 2039
BalI	TGGCCA	3 cut(s)	540, 1518, 1674
BamHI	GGATCC	2 cut(s)	216, 696
BanI	GGYRCC	3 cut(s)	765, 1029, 1798
BanII	GRGCYC	1 cut(s)	732
BbsI	GAAGAC	1 cut(s)	259
Bbv12I	GWGCWC	5 cut(s)	710, 1229, 1303, 2084, 2421
BbvI	GCAGC	3 cut(s)	871, 1966, 2165
BccI	CCATC	6 cut(s)	1137, 1202, 1282, 1309, 1369, 1810
BceAI	ACGGC	6 cut(s)	153, 547, 724, 817, 886, 2002
BciT130I	CCWGG	7 cut(s)	209, 302, 725, 914, 1623, 1908, 2055
BcnI	CCSGG	4 cut(s)	566, 567, 746, 1034
BcoDI	GTCTC	3 cut(s)	1058, 1156, 1905
BfoI	RGCGCY	1 cut(s)	189
BfuAI	ACCTGC	1 cut(s)	1188
BglI	GCCNNNNNGGC	2 cut(s)	51, 326
Bme18I	GGWCC	6 cut(s)	59, 240, 569, 1436, 1544, 2327
BmeT110I	CYCGRG	2 cut(s)	455, 565
BmrI	ACTGGG	1 cut(s)	1304
BmsI	GCATC	4 cut(s)	291, 996, 2099, 2155
BmuI	ACTGGG	1 cut(s)	1304
BpiI	GAAGAC	1 cut(s)	259
BpmI	CTGGAG	3 cut(s)	230, 1605, 1929
Bpu10I	CCTNAGC	1 cut(s)	1532
BpuEI	CTTGAG	1 cut(s)	1769
BpuMI	CCSGG	4 cut(s)	566, 567, 746, 1034
BsaHI	GRCGYC	2 cut(s)	170, 630
BsaJI	CCNNGG	7 cut(s)	366, 479, 565, 724, 912, 913, 1962
BsaWI	WCCGGW	4 cut(s)	56, 702, 762, 1564
BsaXI	ACNNNNNCTCC	8 cut(s)	5, 17, 35, 47, 782, 812, 1651, 1681
Bsc4I	CCNNNNNNNGG	9 cut(s)	292, 324, 396, 709, 751, 913, 914, 986, 1315
Bse118I	RCCGGY	4 cut(s)	516, 702, 762, 1095
Bse1I	ACTGG	1 cut(s)	1310
Bse3DI	GCAATG	1 cut(s)	544
BseAI	TCCGGA	2 cut(s)	56, 1564
BseBI	CCWGG	7 cut(s)	209, 302, 725, 914, 1623, 1908, 2055
BseDI	CCNNGG	7 cut(s)	366, 479, 565, 724, 912, 913, 1962
BseGI	GGATG	6 cut(s)	173, 282, 1882, 1903, 2050, 2114
BseLI	CCNNNNNNNGG	9 cut(s)	292, 324, 396, 709, 751, 913, 914, 986, 1315
BseMI	GCAATG	1 cut(s)	544
BseMII	CTCAG	3 cut(s)	1024, 1242, 1362
BseNI	ACTGG	1 cut(s)	1310
BsePI	GCGCGC	1 cut(s)	296
BseRI	GAGGAG	5 cut(s)	169, 800, 803, 842, 1545
BseSI	GKGCMC	6 cut(s)	732, 908, 1034, 1274, 1310, 2039
BseX3I	CGGCCG	2 cut(s)	138, 518
BseXI	GCAGC	3 cut(s)	871, 1966, 2165
BseYI	CCCAGC	1 cut(s)	291
BsgI	GTGCAG	1 cut(s)	229
Bsh1236I	CGCG	2 cut(s)	298, 2163
Bsh1285I	CGRYCG	3 cut(s)	141, 521, 1946
BshNI	GGYRCC	3 cut(s)	765, 1029, 1798
BshTI	ACCGGT	2 cut(s)	702, 762
BsiEI	CGRYCG	3 cut(s)	141, 521, 1946
BsiHKAI	GWGCWC	5 cut(s)	710, 1229, 1303, 2084, 2421
BsiHKCI	CYCGRG	2 cut(s)	455, 565
BslFI	GGGAC	4 cut(s)	555, 895, 1184, 1226
BslI	CCNNNNNNNGG	9 cut(s)	292, 324, 396, 709, 751, 913, 914, 986, 1315
BsmAI	GTCTC	3 cut(s)	1058, 1156, 1905
BsmFI	GGGAC	4 cut(s)	555, 895, 1184, 1226
BsmI	GAATGC	2 cut(s)	493, 951
BsoBI	CYCGRG	2 cut(s)	455, 565
Bsp120I	GGGCCC	1 cut(s)	728
Bsp13I	TCCGGA	2 cut(s)	56, 1564
Bsp143I	GATC	8 cut(s)	76, 153, 216, 696, 1378, 1625, 1943, 2176
Bsp19I	CCATGG	1 cut(s)	1962
Bsp68I	TCGCGA	1 cut(s)	2163
BspCNI	CTCAG	3 cut(s)	1023, 1241, 1363
BspEI	TCCGGA	2 cut(s)	56, 1564
BspFNI	CGCG	2 cut(s)	298, 2163
BspMI	ACCTGC	1 cut(s)	1188
BspPI	GGATC	9 cut(s)	84, 148, 211, 224, 691, 704, 1386, 1633, 2171
BspT107I	GGYRCC	3 cut(s)	765, 1029, 1798
BsrBI	CCGCTC	3 cut(s)	214, 808, 2353
BsrDI	GCAATG	1 cut(s)	544
BsrFI	RCCGGY	4 cut(s)	516, 702, 762, 1095
BsrI	ACTGG	1 cut(s)	1310
BssAI	RCCGGY	4 cut(s)	516, 702, 762, 1095
BssECI	CCNNGG	7 cut(s)	366, 479, 565, 724, 912, 913, 1962
BssHII	GCGCGC	1 cut(s)	296
BssMI	GATC	8 cut(s)	76, 153, 216, 696, 1378, 1625, 1943, 2176
BssNI	GRCGYC	2 cut(s)	170, 630
BssT1I	CCWWGG	1 cut(s)	1962
Bst2UI	CCWGG	7 cut(s)	209, 302, 725, 914, 1623, 1908, 2055
Bst4CI	ACNGT	9 cut(s)	440, 1078, 1175, 1224, 1798, 1955, 2098, 2274, 2453
Bst6I	CTCTTC	2 cut(s)	914, 2464
BstACI	GRCGYC	2 cut(s)	170, 630
BstDEI	CTNAG	6 cut(s)	1010, 1166, 1228, 1371, 1532, 1584
BstDSI	CCRYGG	2 cut(s)	479, 1962
BstF5I	GGATG	6 cut(s)	173, 282, 1882, 1903, 2050, 2114
BstFNI	CGCG	2 cut(s)	298, 2163
BstH2I	RGCGCY	1 cut(s)	189
BstHHI	GCGC	4 cut(s)	188, 298, 300, 1197
BstKTI	GATC	8 cut(s)	79, 156, 219, 699, 1381, 1628, 1946, 2179
BstMAI	GTCTC	3 cut(s)	1058, 1156, 1905
BstMBI	GATC	8 cut(s)	76, 153, 216, 696, 1378, 1625, 1943, 2176
BstMCI	CGRYCG	3 cut(s)	141, 521, 1946
BstNI	CCWGG	7 cut(s)	209, 302, 725, 914, 1623, 1908, 2055
BstNSI	RCATGY	6 cut(s)	930, 1146, 1187, 1332, 1338, 1878
BstSLI	GKGCMC	6 cut(s)	732, 908, 1034, 1274, 1310, 2039
BstUI	CGCG	2 cut(s)	298, 2163
BstV1I	GCAGC	3 cut(s)	871, 1966, 2165
BstV2I	GAAGAC	1 cut(s)	259
BstX2I	RGATCY	5 cut(s)	76, 153, 216, 696, 1625
BstYI	RGATCY	5 cut(s)	76, 153, 216, 696, 1625
BstZI	CGGCCG	2 cut(s)	138, 518
BtgI	CCRYGG	2 cut(s)	479, 1962
BtgZI	GCGATG	3 cut(s)	486, 652, 2178
BtsCI	GGATG	6 cut(s)	173, 282, 1882, 1903, 2050, 2114
BtsI	GCAGTG	1 cut(s)	2381
BtsIMutI	CAGTG	3 cut(s)	1317, 1951, 2381
BtuMI	TCGCGA	1 cut(s)	2163
BveI	ACCTGC	1 cut(s)	1188
CaiI	CAGNNNCTG	1 cut(s)	1203
CfoI	GCGC	4 cut(s)	188, 298, 300, 1197
Cfr10I	RCCGGY	4 cut(s)	516, 702, 762, 1095
Cfr9I	CCCGGG	1 cut(s)	565
Csp6I	GTAC	5 cut(s)	406, 760, 870, 1368, 2025
CspAI	ACCGGT	2 cut(s)	702, 762
CviQI	GTAC	5 cut(s)	406, 760, 870, 1368, 2025
DdeI	CTNAG	6 cut(s)	1010, 1166, 1228, 1371, 1532, 1584
DpnI	GATC	8 cut(s)	78, 155, 218, 698, 1380, 1627, 1945, 2178
DpnII	GATC	8 cut(s)	76, 153, 216, 696, 1378, 1625, 1943, 2176
EaeI	YGGCCR	5 cut(s)	138, 518, 538, 1516, 1672
EagI	CGGCCG	2 cut(s)	138, 518
Eam1104I	CTCTTC	2 cut(s)	914, 2464
EarI	CTCTTC	2 cut(s)	914, 2464
EciI	GGCGGA	2 cut(s)	307, 340
EclXI	CGGCCG	2 cut(s)	138, 518
Eco130I	CCWWGG	1 cut(s)	1962
Eco147I	AGGCCT	1 cut(s)	305
Eco24I	GRGCYC	1 cut(s)	732
Eco47I	GGWCC	6 cut(s)	59, 240, 569, 1436, 1544, 2327
Eco52I	CGGCCG	2 cut(s)	138, 518
Eco57I	CTGAAG	1 cut(s)	545
Eco88I	CYCGRG	2 cut(s)	455, 565
EcoO109I	RGGNCCY	4 cut(s)	569, 728, 729, 1997
EcoRI	GAATTC	1 cut(s)	110
EcoRII	CCWGG	7 cut(s)	207, 300, 723, 912, 1621, 1906, 2053
EcoT14I	CCWWGG	1 cut(s)	1962
EcoT22I	ATGCAT	2 cut(s)	284, 951
EcoT38I	GRGCYC	1 cut(s)	732
ErhI	CCWWGG	1 cut(s)	1962
FalI	AAGNNNNNCTT	4 cut(s)	1583, 1615, 1813, 1845
FaqI	GGGAC	4 cut(s)	555, 895, 1184, 1226
FauI	CCCGC	6 cut(s)	232, 318, 344, 744, 890, 1094
FauNDI	CATATG	1 cut(s)	278
FblI	GTMKAC	1 cut(s)	2281
FokI	GGATG	6 cut(s)	160, 269, 1869, 1890, 2037, 2121
FriOI	GRGCYC	1 cut(s)	732
GlaI	GCGC	4 cut(s)	187, 297, 299, 1196
GsaI	CCCAGC	1 cut(s)	295
GsuI	CTGGAG	3 cut(s)	230, 1605, 1929
HaeII	RGCGCY	1 cut(s)	189
HhaI	GCGC	4 cut(s)	188, 298, 300, 1197
Hin1I	GRCGYC	2 cut(s)	170, 630
Hin6I	GCGC	4 cut(s)	186, 296, 298, 1195
HinP1I	GCGC	4 cut(s)	186, 296, 298, 1195
HincII	GTYRAC	1 cut(s)	1844
HindII	GTYRAC	1 cut(s)	1844
HphI	GGTGA	4 cut(s)	1330, 1769, 2061, 2311
Hpy166II	GTNNAC	4 cut(s)	760, 870, 1844, 2282
Hpy188III	TCNNGA	9 cut(s)	57, 166, 286, 379, 457, 1133, 1565, 1786, 2162
Hpy8I	GTNNAC	4 cut(s)	760, 870, 1844, 2282
HpyAV	CCTTC	7 cut(s)	350, 619, 673, 775, 787, 835, 2296
HpyCH4III	ACNGT	9 cut(s)	440, 1078, 1175, 1224, 1798, 1955, 2098, 2274, 2453
HpyCH4IV	ACGT	4 cut(s)	170, 630, 1574, 2489
HpyF3I	CTNAG	6 cut(s)	1010, 1166, 1228, 1371, 1532, 1584
HpySE526I	ACGT	4 cut(s)	170, 630, 1574, 2489
Hsp92I	GRCGYC	2 cut(s)	170, 630
HspAI	GCGC	4 cut(s)	186, 296, 298, 1195
KflI	GGGWCCC	1 cut(s)	569
Kpn2I	TCCGGA	2 cut(s)	56, 1564
Kzo9I	GATC	8 cut(s)	76, 153, 216, 696, 1378, 1625, 1943, 2176
LmnI	GCTCC	7 cut(s)	59, 211, 829, 1104, 1306, 1469, 1867
Lsp1109I	GCAGC	3 cut(s)	871, 1966, 2165
LweI	GCATC	4 cut(s)	291, 996, 2099, 2155
MaeII	ACGT	4 cut(s)	170, 630, 1574, 2489
MaeIII	GTNAC	8 cut(s)	1318, 1775, 1949, 2003, 2092, 2138, 2365, 2453
MalI	GATC	8 cut(s)	78, 155, 218, 698, 1380, 1627, 1945, 2178
MbiI	CCGCTC	3 cut(s)	214, 808, 2353
MboI	GATC	8 cut(s)	76, 153, 216, 696, 1378, 1625, 1943, 2176
MboII	GAAGA	9 cut(s)	259, 790, 931, 1254, 1514, 1600, 1603, 2350, 2451
MfeI	CAATTG	1 cut(s)	1522
MflI	RGATCY	5 cut(s)	76, 153, 216, 696, 1625
MlsI	TGGCCA	3 cut(s)	540, 1518, 1674
MluNI	TGGCCA	3 cut(s)	540, 1518, 1674
MlyI	GAGTC	4 cut(s)	14, 26, 80, 2208
MmeI	TCCRAC	5 cut(s)	48, 60, 108, 1834, 2256
Mox20I	TGGCCA	3 cut(s)	540, 1518, 1674
Mph1103I	ATGCAT	2 cut(s)	284, 951
MroI	TCCGGA	2 cut(s)	56, 1564
MscI	TGGCCA	3 cut(s)	540, 1518, 1674
MseI	TTAA	4 cut(s)	648, 1086, 2193, 2256
MslI	CAYNNNNRTG	6 cut(s)	534, 1279, 1290, 1333, 1456, 1608
Msp20I	TGGCCA	3 cut(s)	540, 1518, 1674
MspA1I	CMGCKG	1 cut(s)	523
MunI	CAATTG	1 cut(s)	1522
Mva1269I	GAATGC	2 cut(s)	493, 951
MvaI	CCWGG	7 cut(s)	209, 302, 725, 914, 1623, 1908, 2055
MvnI	CGCG	2 cut(s)	298, 2163
NciI	CCSGG	4 cut(s)	566, 567, 746, 1034
NcoI	CCATGG	1 cut(s)	1962
NdeI	CATATG	1 cut(s)	278
NdeII	GATC	8 cut(s)	76, 153, 216, 696, 1378, 1625, 1943, 2176
NmeAIII	GCCGAG	1 cut(s)	391
NmuCI	GTSAC	6 cut(s)	1318, 1775, 1949, 2003, 2138, 2365
NruI	TCGCGA	1 cut(s)	2163
NsiI	ATGCAT	2 cut(s)	284, 951
NspI	RCATGY	6 cut(s)	930, 1146, 1187, 1332, 1338, 1878
OliI	CACNNNNGTG	1 cut(s)	534
PaeI	GCATGC	3 cut(s)	1146, 1187, 1338
PaeR7I	CTCGAG	1 cut(s)	455
PalAI	GGCGCGCC	1 cut(s)	296
PaqCI	CACCTGC	1 cut(s)	1188
PasI	CCCWGGG	1 cut(s)	913
PauI	GCGCGC	1 cut(s)	296
PceI	AGGCCT	1 cut(s)	305
PciI	ACATGT	1 cut(s)	1328
PctI	GAATGC	2 cut(s)	493, 951
PfeI	GAWTC	7 cut(s)	599, 1561, 1613, 1789, 2197, 2261, 2306
PflFI	GACNNNGTC	1 cut(s)	238
PfoI	TCCNGGA	2 cut(s)	1621, 1906
PinAI	ACCGGT	2 cut(s)	702, 762
Ple19I	CGATCG	1 cut(s)	1946
PleI	GAGTC	4 cut(s)	14, 26, 80, 2208
PpsI	GAGTC	4 cut(s)	14, 26, 80, 2208
PpuMI	RGGWCCY	1 cut(s)	569
PscI	ACATGT	1 cut(s)	1328
Psp5II	RGGWCCY	1 cut(s)	569
Psp6I	CCWGG	7 cut(s)	207, 300, 723, 912, 1621, 1906, 2053
PspFI	CCCAGC	1 cut(s)	291
PspGI	CCWGG	7 cut(s)	207, 300, 723, 912, 1621, 1906, 2053
PspOMI	GGGCCC	1 cut(s)	728
PspPPI	RGGWCCY	1 cut(s)	569
PstNI	CAGNNNCTG	1 cut(s)	1203
PsuI	RGATCY	5 cut(s)	76, 153, 216, 696, 1625
PsyI	GACNNNGTC	1 cut(s)	238
PteI	GCGCGC	1 cut(s)	296
PvuI	CGATCG	1 cut(s)	1946
RruI	TCGCGA	1 cut(s)	2163
RsaI	GTAC	5 cut(s)	407, 761, 871, 1369, 2026
RsaNI	GTAC	5 cut(s)	406, 760, 870, 1368, 2025
RseI	CAYNNNNRTG	6 cut(s)	534, 1279, 1290, 1333, 1456, 1608
SaqAI	TTAA	4 cut(s)	648, 1086, 2193, 2256
Sau3AI	GATC	8 cut(s)	76, 153, 216, 696, 1378, 1625, 1943, 2176
SchI	GAGTC	4 cut(s)	14, 26, 80, 2208
SfaNI	GCATC	4 cut(s)	291, 996, 2099, 2155
Sfr274I	CTCGAG	1 cut(s)	455
SgrAI	CRCCGGYG	1 cut(s)	702
SgsI	GGCGCGCC	1 cut(s)	296
SinI	GGWCC	6 cut(s)	59, 240, 569, 1436, 1544, 2327
SlaI	CTCGAG	1 cut(s)	455
SmaI	CCCGGG	1 cut(s)	567
SmiMI	CAYNNNNRTG	6 cut(s)	534, 1279, 1290, 1333, 1456, 1608
SmlI	CTYRAG	2 cut(s)	455, 1784
SmoI	CTYRAG	2 cut(s)	455, 1784
SphI	GCATGC	3 cut(s)	1146, 1187, 1338
SseBI	AGGCCT	1 cut(s)	305
StuI	AGGCCT	1 cut(s)	305
StyI	CCWWGG	1 cut(s)	1962
TaaI	ACNGT	9 cut(s)	440, 1078, 1175, 1224, 1798, 1955, 2098, 2274, 2453
TaiI	ACGT	4 cut(s)	173, 633, 1577, 2492
TaqI	TCGA	2 cut(s)	456, 2378
TaqII	GACCGA	1 cut(s)	2429
TatI	WGTACW	1 cut(s)	2024
TauI	GCSGC	7 cut(s)	365, 493, 504, 523, 811, 1020, 1183
TfiI	GAWTC	7 cut(s)	599, 1561, 1613, 1789, 2197, 2261, 2306
Tru1I	TTAA	4 cut(s)	648, 1086, 2193, 2256
Tru9I	TTAA	4 cut(s)	648, 1086, 2193, 2256
TscAI	CASTG	3 cut(s)	1317, 1958, 2388
TseFI	GTSAC	6 cut(s)	1318, 1775, 1949, 2003, 2138, 2365
TseI	GCWGC	3 cut(s)	859, 1979, 2153
Tsp45I	GTSAC	6 cut(s)	1318, 1775, 1949, 2003, 2138, 2365
TspDTI	ATGAA	5 cut(s)	1592, 1958, 1977, 2055, 2451
TspGWI	ACGGA	4 cut(s)	241, 253, 1293, 2090
TspMI	CCCGGG	1 cut(s)	565
TspRI	CASTG	3 cut(s)	1317, 1958, 2388
Tth111I	GACNNNGTC	1 cut(s)	238
VpaK11BI	GGWCC	6 cut(s)	59, 240, 569, 1436, 1544, 2327
XapI	RAATTY	3 cut(s)	110, 1441, 1568
XceI	RCATGY	6 cut(s)	930, 1146, 1187, 1332, 1338, 1878
XcmI	CCANNNNNNNNNTGG	2 cut(s)	47, 1653
XhoI	CTCGAG	1 cut(s)	455
XmaI	CCCGGG	1 cut(s)	565
XmiI	GTMKAC	1 cut(s)	2281
ZraI	GACGTC	2 cut(s)	171, 631
Zsp2I	ATGCAT	2 cut(s)	284, 951

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.