Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0010326

Physical Location & Seq

Genomic Coordinates Forward (+)

22234878 .. 22239500

Gene Structure 4623 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom07g20050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2703 bp

ATGGACTTTCCATGTGCCGACGTGCCGTGGCGCTCAAAATCTCCGAAATCTGCCGCGCTCTGTGAAACAAGGAAAAGATCGACTCTTGGCCCCGAAATTCCCAAAATGCCCTCGAACCACTCGGACTCCGACACCGACTCCGACCAGAGCCAATCCGGCTCCGGACCGGTTCGCCACTCGGATCTCTCCGACTCCATTTTCCGAACCTACTTGGAATTCACAGGAAAATCAACAGCGACCACCGCCGACCTCTCCAAGATCCAGTCTTTCCTGACGTCATCATCCTCCGGCGCTCTGTCATGCCTGATATGCCTGGAGCGGATCCGGCCCGCCGACCCGACCTGGTCCTGCACCTCCGTCTGTTTCTCCGTCTTCCACCTGATCTGCATCCAGAGCTGGGCGCGCCAGGCATCCGATTTGTCCGCCTTGCGGGCCTCCACTCGCCTCCCCATTTCCGCCCAGAAAGCCGCCGAGGTATCAGTCTGGAACTGCCCCAAATGTAGGATAGAGTACCCCCATTCCCAAATCCCCAAGAATTATTACTGCTTCTGTGGAAAGCTCGAGAACCCACCGAGCGATGACCCGTGGGTTCTGCCGCATTCGTGCGGCGAGGTCTGCAATCGGCCGCTGAAGCACGGCTGCGGCCACCATTGCTTGCTTCTTTGCCACCCGGGTCCCTGCCCGTCCTGCCCGAAGCTCGTAGAATCTCGGTGCTTTTGTGGGTCTGTGAAGGACGTCCGGCGATGTGGGTTTAAGCATTTCTCTTGTAACAATGCTTGTAAGAAGGTATTGGCTTGTGGGATCCACCGGTGCTCGGAGATTTGCCACCAGGGGCCCTGCTCGCCGTGCCGGGTGCGGGGAGTGTACCGGTGCCAATGTGGGAAGAAGGAGGAGGAGAAGGAGTGTTGTGAGCGGCTTGATTTTAGGTGTGAGGAGCCGTGCGAGAAGGTGCTTGGCTGTGGGCAGCATAAGTGTACCAAAGGGTGTCATTTGGGGGAGTGCGGACCGTGCCCGTCCCAGGGCAAGAGGACATGCCCCTGTGGAAAGAGAATGCATGAGGGATTGTCTTGCAAAGCCTCTGTGCCTTTGTGTGGTTCAACTTGTGATAAGATGCTCAGTTGTGGCTACCATAGGTGCCCGGAGAGGTGTCATCGTGGGGAGTGCATTGAGACTTGCAGAACAGTTGTCATCAAGTCGTGCCGGTGTGGGAGCTTGAAAAAGGAGGTTCCTTGCCATCAAGATTTAGCATGCGAAAGGAAGTGCCAGAGACTAAGGGACTGTGGGCGGCATGCTTGTAAGCGCCGGTGCTGTGATGGGGACTGCCCACCGTGCTCAGAGATTTGTGGAAGAAAGCTACGATGTAGGAACCACAAGTGCCCATCTCCATGCCATCGAGGTGCTTGTGCTCCGTGCCCAGTGATGGTGACAATTATATGTGCATGCGGTCAGACACACTTTGAGGTTCCTTGCGGTACTGAGATGGATCAAAAGCCTCCCAGATGTCGTAAGCCATGCCCTATTACTCCTTTATGCAGGCATGGACCAAATTCCAAGCCGCATAAGTGCCATTATGGAGCCTGCCACCCGTGTAGATTACTTTGTGAGGAAGAATACCCATGTGGCCACAAGTGCAAGCTAAGGTGTCATGGTCCTAAACCTCCTCCGAATCTGGAATTTACGTTGAAACCTAAGAAGAAGAAGTCCTTTCATCAGAGTGAATCTACTCCAGGATCTCCATGCCCTCCATGCCCAGAACTTGTTTGGAGGTCATGTGTTGGCCAGCACATTGGAGCAGATAAAATGATGGTCTGCTCAGATAACACACTATTTTCCTGCGAAAACTTGTGCGGAAATCCTCTACCTTGTGGTAATCACTTTTGCACAAAAACTTGTCACCCCCTCAAGAATCAAACGGTGCCATCAGTCCGGCAAGCAAGAAGTGAGCCTTGTGAGAAGTGTCAACTTCCTTGCAAAAAGGAGAGGGAGCCTGCATGTCCTCATCCTTGCCCCTTGCCATGTCATCCTGGAGACTGCCCTCCTTGCAAGGTGCTTGTAAAGCGATCGTGTCACTGTGGTTCCATGGTTCATGTTTTTGAGTGCATATATTTTAACAGCTTGTCCGAAAAGGAGCAAATGACTGCTCGTTCATGTAAGGGGCCTTGTCACAGAAAGTTGCCGTATTGTACTCATCTGTGCCCAGAGATTTGTCATCCTGGTCAATGTTCCTCACCCGAAAAGTGCTCTAAAAAGGTTACCATCCGTTGTGGATGCCAGAGCTTGAAAAAGGAGTGGTTATGTCACAATGTTCAGGCAGCCTATCGCGATGCTGGTTCTGATCCCAAAGATGCATCTAAGAATCAATTTGGACTTGGACTCATTCCTTGCAATTCCGACTGCAAAAGCAAACAAAAGGTTAATGATTCAGAATTACAGTTGCGTAGACCTAAATCCGTGGAGGTGAAGGAATCAGATAATGATAGGCAAGGACCAAAGAGGAAGAAAAGGCGTGAGCGGGTGCAACAAGTGACTCAAACCTCGACACTGCAAAAAATTATTTCCAACGTGAAACGGTGTCTTCTAGTTTTCACCCTCCTGGTGGTTTTAGCTGCTGTATCATACTACGGATACATGGGTCTCCTCCGGCTTAATGATTGGATGAATGAAGTTGAAGAGAATCGACAAAGAAGATACCCCCGAATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0000976 GO:0000977 GO:0000981 GO:0000982 GO:0001012 GO:0001067 GO:0001078 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009628 GO:0009651 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010310 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051193 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000377 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-NF-X1

Protein Analysis

901

Amino Acids

100.45

Weight (kDa)

8.86

Isoelectric Point (pI)

62.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-NF-X1	PF01422	266 - 284	8e-06	NF-X1 type zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010326)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05660
fragaria_vesca	FvH4_7g24480
malus_domestica	MD00G1166700.v1.1 MD07G1222000.v1.1
prunus_persica	Prupe.2G250200_v2.0.a1
pyrus_communis	pycom07g20050
rosa_chinensis	RchiOBHm_Chr1g0370241
rosa_laevigata	RLG00000027066
rosa_multiflora	Rmu_co8251429.1_g000001 Rmu_co8258023.1_g000001 Rmu_sc0018500.1_g000001
rosa_roxburghii	Rroxscaffold_4G00286720 Rroxscaffold_4G00287290
rosa_rugosa	Rorug01G0354900 Rorug01G0355000 Rorug01G0355100
rosa_samantha	Rh1AG363700 Rh1BG326800 Rh1CG340100 Rh1DG357200
rosa_wichuraiana	Rw1G032000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	278, 738
AccB1I	GGYRCC	3 cut(s)	870, 1134, 1915
AccBSI	CCGCTC	3 cut(s)	319, 913, 2512
AccI	GTMKAC	1 cut(s)	2440
AccII	CGCG	3 cut(s)	56, 403, 2322
AccIII	TCCGGA	1 cut(s)	161
AclWI	GGATC	9 cut(s)	189, 253, 316, 329, 796, 809, 1491, 1738, 2330
AcoI	YGGCCR	4 cut(s)	623, 643, 1621, 1777
AcsI	RAATTY	4 cut(s)	96, 215, 1546, 1673
AcuI	CTGAAG	1 cut(s)	650
AcyI	GRCGYC	2 cut(s)	275, 735
AfaI	GTAC	5 cut(s)	512, 866, 976, 1474, 2185
AgeI	ACCGGT	3 cut(s)	166, 807, 867
AgsI	TTSAA	5 cut(s)	1098, 1216, 1684, 2281, 2669
AjiI	CACGTC	1 cut(s)	22
AjnI	CCWGG	9 cut(s)	312, 341, 405, 828, 1017, 1726, 2023, 2212, 2592
AluBI	AGCT	9 cut(s)	396, 559, 697, 1212, 1354, 1636, 2115, 2277, 2606
AluI	AGCT	9 cut(s)	396, 559, 697, 1212, 1354, 1636, 2115, 2277, 2606
Alw21I	GWGCWC	4 cut(s)	815, 1334, 1408, 2243
Alw26I	GTCTC	4 cut(s)	1163, 1261, 2022, 2639
AlwI	GGATC	9 cut(s)	189, 253, 316, 329, 796, 809, 1491, 1738, 2330
Ama87I	CYCGRG	2 cut(s)	560, 670
Aor13HI	TCCGGA	1 cut(s)	161
AoxI	GGCC	9 cut(s)	88, 326, 432, 623, 643, 833, 1621, 1777, 2156
ApaI	GGGCCC	1 cut(s)	837
ApeKI	GCWGC	4 cut(s)	639, 964, 2312, 2606
ApoI	RAATTY	4 cut(s)	96, 215, 1546, 1673
AscI	GGCGCGCC	1 cut(s)	401
AsiGI	ACCGGT	3 cut(s)	166, 807, 867
AspLEI	GCGC	6 cut(s)	33, 58, 293, 403, 405, 1302
AsuC2I	CCSGG	4 cut(s)	671, 672, 851, 1139
AsuHPI	GGTGA	5 cut(s)	1435, 1886, 2220, 2470, 2578
AvaI	CYCGRG	2 cut(s)	560, 670
AvaII	GGWCC	7 cut(s)	164, 345, 674, 1004, 1541, 1649, 2486
BaeGI	GKGCMC	6 cut(s)	837, 1013, 1139, 1379, 1415, 2198
BaeI	ACNNNNGTAYC	2 cut(s)	2617, 2650
BalI	TGGCCA	2 cut(s)	1623, 1779
BamHI	GGATCC	2 cut(s)	321, 801
BanI	GGYRCC	3 cut(s)	870, 1134, 1915
BanII	GRGCYC	1 cut(s)	837
BbsI	GAAGAC	2 cut(s)	364, 2567
Bbv12I	GWGCWC	4 cut(s)	815, 1334, 1408, 2243
BbvI	GCAGC	4 cut(s)	626, 976, 2324, 2593
BccI	CCATC	9 cut(s)	1242, 1307, 1387, 1398, 1414, 1474, 1798, 1927, 2264
BceAI	ACGGC	5 cut(s)	10, 652, 829, 922, 2161
BciT130I	CCWGG	9 cut(s)	314, 343, 407, 830, 1019, 1728, 2025, 2214, 2594
BciVI	GTATCC	1 cut(s)	2618
BcnI	CCSGG	4 cut(s)	671, 672, 851, 1139
BcoDI	GTCTC	4 cut(s)	1163, 1261, 2022, 2639
BfaI	CTAG	1 cut(s)	2579
BfoI	RGCGCY	3 cut(s)	34, 294, 1303
BfuI	GTATCC	1 cut(s)	2618
BglI	GCCNNNNNGGC	2 cut(s)	156, 431
Bme18I	GGWCC	7 cut(s)	164, 345, 674, 1004, 1541, 1649, 2486
BmeT110I	CYCGRG	2 cut(s)	560, 670
BmgBI	CACGTC	1 cut(s)	22
BmrI	ACTGGG	1 cut(s)	1409
BmsI	GCATC	7 cut(s)	396, 419, 1101, 2258, 2314, 2335, 2357
BmuI	ACTGGG	1 cut(s)	1409
BpiI	GAAGAC	2 cut(s)	364, 2567
BpmI	CTGGAG	3 cut(s)	335, 1710, 2046
Bpu10I	CCTNAGC	1 cut(s)	1637
BpuEI	CTTGAG	1 cut(s)	1886
BpuMI	CCSGG	4 cut(s)	671, 672, 851, 1139
BsaBI	GATNNNNATC	1 cut(s)	386
BsaHI	GRCGYC	2 cut(s)	275, 735
BsaI	GGTCTC	1 cut(s)	2639
BsaJI	CCNNGG	9 cut(s)	26, 471, 584, 670, 829, 1017, 1018, 2079, 2451
BsaWI	WCCGGW	4 cut(s)	161, 166, 807, 867
BsaXI	ACNNNNNCTCC	8 cut(s)	110, 122, 140, 152, 887, 917, 1756, 1786
Bse118I	RCCGGY	5 cut(s)	166, 807, 867, 1200, 1302
Bse1I	ACTGG	2 cut(s)	262, 1415
Bse3DI	GCAATG	1 cut(s)	649
Bse8I	GATNNNNATC	1 cut(s)	386
BseAI	TCCGGA	1 cut(s)	161
BseBI	CCWGG	9 cut(s)	314, 343, 407, 830, 1019, 1728, 2025, 2214, 2594
BseDI	CCNNGG	9 cut(s)	26, 471, 584, 670, 829, 1017, 1018, 2079, 2451
BseGI	GGATG	9 cut(s)	281, 387, 410, 1999, 2020, 2209, 2256, 2273, 2661
BseJI	GATNNNNATC	1 cut(s)	386
BseMI	GCAATG	1 cut(s)	649
BseMII	CTCAG	4 cut(s)	1129, 1347, 1467, 1827
BseNI	ACTGG	2 cut(s)	262, 1415
BsePI	GCGCGC	1 cut(s)	401
BseRI	GAGGAG	5 cut(s)	905, 908, 947, 1650, 2627
BseSI	GKGCMC	6 cut(s)	837, 1013, 1139, 1379, 1415, 2198
BseX3I	CGGCCG	1 cut(s)	623
BseXI	GCAGC	4 cut(s)	626, 976, 2324, 2593
BseYI	CCCAGC	1 cut(s)	396
BsgI	GTGCAG	1 cut(s)	334
Bsh1236I	CGCG	3 cut(s)	56, 403, 2322
Bsh1285I	CGRYCG	2 cut(s)	626, 2063
BshFI	GGCC	9 cut(s)	90, 328, 434, 625, 645, 835, 1623, 1779, 2158
BshNI	GGYRCC	3 cut(s)	870, 1134, 1915
BshTI	ACCGGT	3 cut(s)	166, 807, 867
BsiEI	CGRYCG	2 cut(s)	626, 2063
BsiHKAI	GWGCWC	4 cut(s)	815, 1334, 1408, 2243
BsiHKCI	CYCGRG	2 cut(s)	560, 670
BslFI	GGGAC	4 cut(s)	660, 1000, 1289, 1331
BsmAI	GTCTC	4 cut(s)	1163, 1261, 2022, 2639
BsmFI	GGGAC	4 cut(s)	660, 1000, 1289, 1331
BsmI	GAATGC	2 cut(s)	598, 1056
BsnI	GGCC	9 cut(s)	90, 328, 434, 625, 645, 835, 1623, 1779, 2158
Bso31I	GGTCTC	1 cut(s)	2639
BsoBI	CYCGRG	2 cut(s)	560, 670
Bsp120I	GGGCCC	1 cut(s)	833
Bsp13I	TCCGGA	1 cut(s)	161
Bsp19I	CCATGG	1 cut(s)	2079
Bsp68I	TCGCGA	1 cut(s)	2322
BspANI	GGCC	9 cut(s)	90, 328, 434, 625, 645, 835, 1623, 1779, 2158
BspCNI	CTCAG	4 cut(s)	1128, 1346, 1468, 1826
BspEI	TCCGGA	1 cut(s)	161
BspFNI	CGCG	3 cut(s)	56, 403, 2322
BspPI	GGATC	9 cut(s)	189, 253, 316, 329, 796, 809, 1491, 1738, 2330
BspT107I	GGYRCC	3 cut(s)	870, 1134, 1915
BspTNI	GGTCTC	1 cut(s)	2639
BsrBI	CCGCTC	3 cut(s)	319, 913, 2512
BsrDI	GCAATG	1 cut(s)	649
BsrFI	RCCGGY	5 cut(s)	166, 807, 867, 1200, 1302
BsrI	ACTGG	2 cut(s)	262, 1415
BssAI	RCCGGY	5 cut(s)	166, 807, 867, 1200, 1302
BssECI	CCNNGG	9 cut(s)	26, 471, 584, 670, 829, 1017, 1018, 2079, 2451
BssHII	GCGCGC	1 cut(s)	401
BssNI	GRCGYC	2 cut(s)	275, 735
BssT1I	CCWWGG	1 cut(s)	2079
Bst2UI	CCWGG	9 cut(s)	314, 343, 407, 830, 1019, 1728, 2025, 2214, 2594
Bst4CI	ACNGT	8 cut(s)	1008, 1183, 1280, 1329, 1915, 2072, 2433, 2571
Bst6I	CTCTTC	1 cut(s)	2664
BstACI	GRCGYC	2 cut(s)	275, 735
BstDEI	CTNAG	8 cut(s)	1115, 1271, 1333, 1476, 1637, 1689, 1813, 2352
BstDSI	CCRYGG	4 cut(s)	26, 584, 2079, 2451
BstEII	GGTNACC	1 cut(s)	2251
BstF5I	GGATG	9 cut(s)	281, 387, 410, 1999, 2020, 2209, 2256, 2273, 2661
BstFNI	CGCG	3 cut(s)	56, 403, 2322
BstH2I	RGCGCY	3 cut(s)	34, 294, 1303
BstHHI	GCGC	6 cut(s)	33, 58, 293, 403, 405, 1302
BstMAI	GTCTC	4 cut(s)	1163, 1261, 2022, 2639
BstMCI	CGRYCG	2 cut(s)	626, 2063
BstNI	CCWGG	9 cut(s)	314, 343, 407, 830, 1019, 1728, 2025, 2214, 2594
BstNSI	RCATGY	5 cut(s)	1035, 1251, 1292, 1443, 1995
BstPI	GGTNACC	1 cut(s)	2251
BstSLI	GKGCMC	6 cut(s)	837, 1013, 1139, 1379, 1415, 2198
BstUI	CGCG	3 cut(s)	56, 403, 2322
BstV1I	GCAGC	4 cut(s)	626, 976, 2324, 2593
BstV2I	GAAGAC	2 cut(s)	364, 2567
BstX2I	RGATCY	5 cut(s)	181, 258, 321, 801, 1730
BstXI	CCANNNNNNTGG	1 cut(s)	1787
BstYI	RGATCY	5 cut(s)	181, 258, 321, 801, 1730
BstZI	CGGCCG	1 cut(s)	623
BsuI	GTATCC	1 cut(s)	2618
BsuRI	GGCC	9 cut(s)	90, 328, 434, 625, 645, 835, 1623, 1779, 2158
BtgI	CCRYGG	4 cut(s)	26, 584, 2079, 2451
BtgZI	GCGATG	3 cut(s)	591, 757, 2337
BtrI	CACGTC	1 cut(s)	22
BtsCI	GGATG	9 cut(s)	281, 387, 410, 1999, 2020, 2209, 2256, 2273, 2661
BtsI	GCAGTG	1 cut(s)	2540
BtsIMutI	CAGTG	3 cut(s)	1422, 2068, 2540
BtuMI	TCGCGA	1 cut(s)	2322
CfoI	GCGC	6 cut(s)	33, 58, 293, 403, 405, 1302
Cfr10I	RCCGGY	5 cut(s)	166, 807, 867, 1200, 1302
Cfr9I	CCCGGG	1 cut(s)	670
CpoI	CGGWCCG	2 cut(s)	164, 1004
CsiI	ACCWGGT	1 cut(s)	341
Csp6I	GTAC	5 cut(s)	511, 865, 975, 1473, 2184
CspAI	ACCGGT	3 cut(s)	166, 807, 867
CspI	CGGWCCG	2 cut(s)	164, 1004
CviQI	GTAC	5 cut(s)	511, 865, 975, 1473, 2184
DdeI	CTNAG	8 cut(s)	1115, 1271, 1333, 1476, 1637, 1689, 1813, 2352
EaeI	YGGCCR	4 cut(s)	623, 643, 1621, 1777
EagI	CGGCCG	1 cut(s)	623
Eam1104I	CTCTTC	1 cut(s)	2664
EarI	CTCTTC	1 cut(s)	2664
EciI	GGCGGA	2 cut(s)	412, 445
EclXI	CGGCCG	1 cut(s)	623
Eco130I	CCWWGG	1 cut(s)	2079
Eco24I	GRGCYC	1 cut(s)	837
Eco31I	GGTCTC	1 cut(s)	2639
Eco47I	GGWCC	7 cut(s)	164, 345, 674, 1004, 1541, 1649, 2486
Eco52I	CGGCCG	1 cut(s)	623
Eco57I	CTGAAG	1 cut(s)	650
Eco88I	CYCGRG	2 cut(s)	560, 670
Eco91I	GGTNACC	1 cut(s)	2251
EcoO109I	RGGNCCY	4 cut(s)	674, 833, 834, 2156
EcoO65I	GGTNACC	1 cut(s)	2251
EcoRI	GAATTC	1 cut(s)	215
EcoRII	CCWGG	9 cut(s)	312, 341, 405, 828, 1017, 1726, 2023, 2212, 2592
EcoT14I	CCWWGG	1 cut(s)	2079
EcoT22I	ATGCAT	2 cut(s)	1056, 2350
EcoT38I	GRGCYC	1 cut(s)	837
ErhI	CCWWGG	1 cut(s)	2079
FalI	AAGNNNNNCTT	4 cut(s)	1688, 1720, 1930, 1962
FaqI	GGGAC	4 cut(s)	660, 1000, 1289, 1331
FauI	CCCGC	4 cut(s)	337, 423, 849, 2505
FblI	GTMKAC	1 cut(s)	2440
FokI	GGATG	9 cut(s)	268, 374, 397, 1986, 2007, 2196, 2243, 2280, 2668
FriOI	GRGCYC	1 cut(s)	837
FspBI	CTAG	1 cut(s)	2579
GlaI	GCGC	6 cut(s)	32, 57, 292, 402, 404, 1301
GsaI	CCCAGC	1 cut(s)	400
GsuI	CTGGAG	3 cut(s)	335, 1710, 2046
HaeII	RGCGCY	3 cut(s)	34, 294, 1303
HaeIII	GGCC	9 cut(s)	90, 328, 434, 625, 645, 835, 1623, 1779, 2158
HhaI	GCGC	6 cut(s)	33, 58, 293, 403, 405, 1302
Hin1I	GRCGYC	2 cut(s)	275, 735
Hin6I	GCGC	6 cut(s)	31, 56, 291, 401, 403, 1300
HinP1I	GCGC	6 cut(s)	31, 56, 291, 401, 403, 1300
HincII	GTYRAC	1 cut(s)	1961
HindII	GTYRAC	1 cut(s)	1961
HphI	GGTGA	5 cut(s)	1435, 1886, 2220, 2470, 2578
Hpy166II	GTNNAC	4 cut(s)	865, 975, 1961, 2441
Hpy188III	TCNNGA	9 cut(s)	162, 271, 391, 484, 562, 1238, 1670, 1903, 2321
Hpy8I	GTNNAC	4 cut(s)	865, 975, 1961, 2441
Hpy99I	CGWCG	1 cut(s)	23
HpyAV	CCTTC	6 cut(s)	724, 778, 880, 892, 940, 2455
HpyCH4III	ACNGT	8 cut(s)	1008, 1183, 1280, 1329, 1915, 2072, 2433, 2571
HpyCH4IV	ACGT	5 cut(s)	21, 275, 735, 1679, 2562
HpyF3I	CTNAG	8 cut(s)	1115, 1271, 1333, 1476, 1637, 1689, 1813, 2352
HpySE526I	ACGT	5 cut(s)	21, 275, 735, 1679, 2562
Hsp92I	GRCGYC	2 cut(s)	275, 735
HspAI	GCGC	6 cut(s)	31, 56, 291, 401, 403, 1300
KflI	GGGWCCC	1 cut(s)	674
Kpn2I	TCCGGA	1 cut(s)	161
LmnI	GCTCC	9 cut(s)	164, 316, 934, 1209, 1411, 1574, 1790, 1984, 2128
Lsp1109I	GCAGC	4 cut(s)	626, 976, 2324, 2593
LweI	GCATC	7 cut(s)	396, 419, 1101, 2258, 2314, 2335, 2357
MabI	ACCWGGT	1 cut(s)	341
MaeI	CTAG	1 cut(s)	2579
MaeII	ACGT	5 cut(s)	21, 275, 735, 1679, 2562
MaeIII	GTNAC	8 cut(s)	767, 1423, 1892, 2066, 2162, 2251, 2297, 2524
MbiI	CCGCTC	3 cut(s)	319, 913, 2512
MflI	RGATCY	5 cut(s)	181, 258, 321, 801, 1730
MlsI	TGGCCA	2 cut(s)	1623, 1779
MluNI	TGGCCA	2 cut(s)	1623, 1779
MlyI	GAGTC	6 cut(s)	76, 119, 131, 185, 2367, 2521
MmeI	TCCRAC	5 cut(s)	153, 165, 213, 2415, 2583
Mox20I	TGGCCA	2 cut(s)	1623, 1779
Mph1103I	ATGCAT	2 cut(s)	1056, 2350
MroI	TCCGGA	1 cut(s)	161
MscI	TGGCCA	2 cut(s)	1623, 1779
MseI	TTAA	4 cut(s)	753, 2109, 2415, 2646
MslI	CAYNNNNRTG	3 cut(s)	1384, 1395, 1713
Msp20I	TGGCCA	2 cut(s)	1623, 1779
MspA1I	CMGCKG	1 cut(s)	628
Mva1269I	GAATGC	2 cut(s)	598, 1056
MvaI	CCWGG	9 cut(s)	314, 343, 407, 830, 1019, 1728, 2025, 2214, 2594
MvnI	CGCG	3 cut(s)	56, 403, 2322
NciI	CCSGG	4 cut(s)	671, 672, 851, 1139
NcoI	CCATGG	1 cut(s)	2079
NmeAIII	GCCGAG	1 cut(s)	496
NmuCI	GTSAC	6 cut(s)	1423, 1892, 2066, 2162, 2297, 2524
NruI	TCGCGA	1 cut(s)	2322
NsiI	ATGCAT	2 cut(s)	1056, 2350
NspI	RCATGY	5 cut(s)	1035, 1251, 1292, 1443, 1995
PaeI	GCATGC	3 cut(s)	1251, 1292, 1443
PaeR7I	CTCGAG	1 cut(s)	560
PalAI	GGCGCGCC	1 cut(s)	401
PasI	CCCWGGG	1 cut(s)	1018
PauI	GCGCGC	1 cut(s)	401
PctI	GAATGC	2 cut(s)	598, 1056
PfeI	GAWTC	9 cut(s)	704, 1666, 1718, 1906, 2356, 2420, 2465, 2674, 2697
PflFI	GACNNNGTC	1 cut(s)	343
PfoI	TCCNGGA	2 cut(s)	1726, 2023
PinAI	ACCGGT	3 cut(s)	166, 807, 867
Ple19I	CGATCG	1 cut(s)	2063
PleI	GAGTC	6 cut(s)	76, 119, 131, 185, 2367, 2521
PpsI	GAGTC	6 cut(s)	76, 119, 131, 185, 2367, 2521
PpuMI	RGGWCCY	1 cut(s)	674
Psp5II	RGGWCCY	1 cut(s)	674
Psp6I	CCWGG	9 cut(s)	312, 341, 405, 828, 1017, 1726, 2023, 2212, 2592
PspEI	GGTNACC	1 cut(s)	2251
PspFI	CCCAGC	1 cut(s)	396
PspGI	CCWGG	9 cut(s)	312, 341, 405, 828, 1017, 1726, 2023, 2212, 2592
PspOMI	GGGCCC	1 cut(s)	833
PspPPI	RGGWCCY	1 cut(s)	674
PsuI	RGATCY	5 cut(s)	181, 258, 321, 801, 1730
PsyI	GACNNNGTC	1 cut(s)	343
PteI	GCGCGC	1 cut(s)	401
PvuI	CGATCG	1 cut(s)	2063
RruI	TCGCGA	1 cut(s)	2322
RsaI	GTAC	5 cut(s)	512, 866, 976, 1474, 2185
RsaNI	GTAC	5 cut(s)	511, 865, 975, 1473, 2184
RseI	CAYNNNNRTG	3 cut(s)	1384, 1395, 1713
Rsr2I	CGGWCCG	2 cut(s)	164, 1004
RsrII	CGGWCCG	2 cut(s)	164, 1004
SaqAI	TTAA	4 cut(s)	753, 2109, 2415, 2646
SchI	GAGTC	6 cut(s)	76, 119, 131, 185, 2367, 2521
SexAI	ACCWGGT	1 cut(s)	341
SfaNI	GCATC	7 cut(s)	396, 419, 1101, 2258, 2314, 2335, 2357
Sfr274I	CTCGAG	1 cut(s)	560
SgrAI	CRCCGGYG	2 cut(s)	807, 1302
SgsI	GGCGCGCC	1 cut(s)	401
SinI	GGWCC	7 cut(s)	164, 345, 674, 1004, 1541, 1649, 2486
SlaI	CTCGAG	1 cut(s)	560
SmaI	CCCGGG	1 cut(s)	672
SmiMI	CAYNNNNRTG	3 cut(s)	1384, 1395, 1713
SmlI	CTYRAG	2 cut(s)	560, 1901
SmoI	CTYRAG	2 cut(s)	560, 1901
SphI	GCATGC	3 cut(s)	1251, 1292, 1443
SspMI	CTAG	1 cut(s)	2579
StyI	CCWWGG	1 cut(s)	2079
TaaI	ACNGT	8 cut(s)	1008, 1183, 1280, 1329, 1915, 2072, 2433, 2571
TaiI	ACGT	5 cut(s)	24, 278, 738, 1682, 2565
TaqI	TCGA	6 cut(s)	80, 113, 561, 1393, 2537, 2677
TatI	WGTACW	1 cut(s)	2183
TauI	GCSGC	9 cut(s)	56, 470, 598, 609, 628, 645, 916, 1288, 1558
TfiI	GAWTC	9 cut(s)	704, 1666, 1718, 1906, 2356, 2420, 2465, 2674, 2697
Tru1I	TTAA	4 cut(s)	753, 2109, 2415, 2646
Tru9I	TTAA	4 cut(s)	753, 2109, 2415, 2646
TscAI	CASTG	3 cut(s)	1422, 2075, 2547
TseFI	GTSAC	6 cut(s)	1423, 1892, 2066, 2162, 2297, 2524
TseI	GCWGC	4 cut(s)	639, 964, 2312, 2606
Tsp45I	GTSAC	6 cut(s)	1423, 1892, 2066, 2162, 2297, 2524
TspDTI	ATGAA	5 cut(s)	1697, 2075, 2136, 2672, 2676
TspGWI	ACGGA	6 cut(s)	346, 358, 1398, 2249, 2440, 2637
TspMI	CCCGGG	1 cut(s)	670
TspRI	CASTG	3 cut(s)	1422, 2075, 2547
Tth111I	GACNNNGTC	1 cut(s)	343
VpaK11BI	GGWCC	7 cut(s)	164, 345, 674, 1004, 1541, 1649, 2486
XapI	RAATTY	4 cut(s)	96, 215, 1546, 1673
XceI	RCATGY	5 cut(s)	1035, 1251, 1292, 1443, 1995
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1758
XhoI	CTCGAG	1 cut(s)	560
XmaI	CCCGGG	1 cut(s)	670
XmiI	GTMKAC	1 cut(s)	2440
XspI	CTAG	1 cut(s)	2579
ZraI	GACGTC	2 cut(s)	276, 736
Zsp2I	ATGCAT	2 cut(s)	1056, 2350

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.