Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0010326

Physical Location & Seq

Genomic Coordinates Forward (+)

8330673 .. 8335481

Gene Structure 4809 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00287290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2553 bp

ATGAACTCGGACTCCGATTCCGATTCCGATCCCGATCACAGCCAATCCGGATCCGGATCCGGATCCGCACCGACCCGCCGCCACACGGACCTATCTGACTCCATTTTCCGATCCTACTTAGACTTCACCGGAAAAACCTCAGCCACCAACGCCGACCTCTCCAAGATCCAATCCTTCCTGACGTCATCCTCATCCGGCGCTCTGTCATGCTTGATATGCCTGGAGCGGATCCGACCCGGAGACCCGACCTGGTCCTGCACCTCCGTCTGCTTCTCCGTCTTCCACCTCTTCTGCATCCAGAGCTGGGCCCGCCAGGCCTCCGACTTGTCGGCGCTACGCGCATCCACGCGCCTCCCGATTTCGCAGAGCAAAGCGGCGGAGGTGTCACTCTGGAATTGCCCTAAATGTAGAGTAGAGTACACCCAGTCTCAAATCCCAAAGATTTACCGGTGCTTCTGTGGAAAACTCGAGAATCCACCGAGTGATGACCCGTGGATTCTTCCTCATTCGTGCGGTGAGGTCTGTAATAGGCCTCTGAAGCACAATTGCGGCCACCATTGCTTGCTTCTGTGCCATCCGGGGCCCTGCCCGTCCTGCCCGAAGCTCGTGAAGGCTCGCTGCTTCTGTGGCTCTGTCCAGGACATGAGGCGGTGTGGGTTTAAGAATTTCTCATGTAACAATGCTTGTGAGAAGAAATTAGCGTGTGGTGTTCACCGCTGCTCGGAGATTTGCCACGAGGGGCCTTGCCCGCCGTGTCGGGTGAGGGGAGGTATAGGTGCCAATGTGGGAAGAGGGAGGAGGAGAGGGAGTGTTGTGAGAGGCTTGATTTTAGGGGAGTGTGCACCGTGCCCGTTTCAGGGGAAGAGGACATGTCCATGTGGGAAGAGAGTGCATGAAGGATTGTCCTGCAAGGATGCAGTGCCGCTGTGTGGTGCAACTTGTGATAAGGTGTTAAGCTGTGGCTACCATAGGTGCCCGGAGAGGTGTCACCGCGGGCAGTGCCTTGAGACTTGCAGAACTGTTGTCACTAAGAGGTGTCGCTGCGGGGGCTTGAAGAAGGAGGTTCCTTGTCATCAAGATTTGGCATGTGAAAGGAAGTGCCAGAGATTAAGAGACTGTGGGCGGCATCCTTGTAAACGCCGCTGCTGTGATGGGGACTGCCCACCATGCTCAGAGATTTGTGGCAGAAAGCTTCGCTGTAAGAACCACAAATGCCCATCTCCCTGCCATAGAGGTGCTTGTGCTCCTTGCCCAGTGATGGTGACAATTGCATGTTTATGTGGTGCCACACACTTTGAGGTTCCTTGTGGCACTGAGATGGATCAGAAGCCTCCTCGATGTCGTAAGCCATGCCCTATTACTCCTTTATGTAGGCATGGACCAAATAGCAAGCCGCATAAATGCCATTATGGAGCTTGCCACCCATGTAAACTACTTTGTGAAGAAGAATACCCATGTGGCCACAAGTGCAAGCTAAGGTGCCATGGACCTAAACCTCCTCCAAATCCAGAATTTACATTGAAACCGAAGAAAAAGAAGTCTTTTCATCTGCCTGAGTGTACTCCTGGTTCTCCATGCCCTCCATGCCCAGAGCTTGTTTGGAGATCATGTGTGGGCCAGCACTTTGCAGCAGACAGAATGATGGTCTGTTCAGATAAATCACTATTTTCCTGCGATAACTTGTGTGGGAATCCTCTACCTTGTGGTAACCACTTTTGCACCAAAACTTGCCACCCGCTCAAGAATCAGTCTTTGCCATCAGGGCAGCAAGCAAGAAGTGAGCCTTGTGAAGTGTGTTATCTTCCTTGCGAAAAGGAGAGGGCGCCAGCATGTATACATCCTTGCTCCTTGTCATGTCATCCTGGAGACTGCCCTCCTTGCAAAGTGCTCGTTAAGCGATCATGTCACTGTGGTTCCATGGTCCATGTTTTTGAGTGCATATACTATAATAGTTTGTCTGAAAAGGAGCAAACCGCTGTTCGTTCTTGTAAGGGGCTCTGTCATAGAAAGTTGCCTTATTGTACACATCTTTGCCCAGAGACTTGTCATCCTGGTCAGTGCTCATCACCTGAGAAGTGCTCTAAAAAGGTTACTGTTCGTTGTGGATGCCAGACCTTGAAAAGGGAGTGGTTATGTCATGATGTGCAGGCAGCCTATCGCAATGCTGGTACTGATCCCAAAGATATACCTAAGAATCAATTTGGACTTGGACTCCTCCCTTGCGACTCTAACTGCAAAAGTAAACAAAAGGTTGTTGATTCAGAATTGCATTCTCGCAGACCTAAAGTTGTGGAGGTGAAGGAACCAGATACTGATAAGCCTGCACCAAAGAGGAAGAAAAGGCGTGAACGAGGACAAGAAGTGGCACAAATCTCGACACTGCAAAAAATTTTGGCCCACATGAAGCGATTTCTTCTAGTGGTCATCCTTCTGGTGGCTTTAGCTGCAGTATCATATTATGGCTACAGGGGTCTCCTCTGGCTCTCAGATTGGATGAATGAAGTAGAAGAGAAAAGGCAAAGAAGACACCCCGAATCTGATCGACGAGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0000976 GO:0000977 GO:0000981 GO:0000982 GO:0001012 GO:0001067 GO:0001078 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009628 GO:0009651 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010310 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051193 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000377 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-NF-X1

Protein Analysis

850

Amino Acids

94.37

Weight (kDa)

8.94

Isoelectric Point (pI)

60.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-NF-X1	PF01422	235 - 253	1.8e-06	NF-X1 type zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010326)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05660
fragaria_vesca	FvH4_7g24480
malus_domestica	MD00G1166700.v1.1 MD07G1222000.v1.1
prunus_persica	Prupe.2G250200_v2.0.a1
pyrus_communis	pycom07g20050
rosa_chinensis	RchiOBHm_Chr1g0370241
rosa_laevigata	RLG00000027066
rosa_multiflora	Rmu_co8251429.1_g000001 Rmu_co8258023.1_g000001 Rmu_sc0018500.1_g000001
rosa_roxburghii	Rroxscaffold_4G00286720 Rroxscaffold_4G00287290
rosa_rugosa	Rorug01G0354900 Rorug01G0355000 Rorug01G0355100
rosa_samantha	Rh1AG363700 Rh1BG326800 Rh1CG340100 Rh1DG357200
rosa_wichuraiana	Rw1G032000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	185
AccB1I	GGYRCC	5 cut(s)	776, 972, 1283, 1479, 1822
AccBSI	CCGCTC	2 cut(s)	226, 1738
AccI	GTMKAC	1 cut(s)	1834
AccII	CGCG	3 cut(s)	339, 349, 993
AccIII	TCCGGA	3 cut(s)	47, 53, 59
AcoI	YGGCCR	2 cut(s)	550, 1459
AcsI	RAATTY	3 cut(s)	664, 1511, 2388
AcuI	CTGAAG	1 cut(s)	557
AcyI	GRCGYC	2 cut(s)	182, 1823
AdeI	CACNNNGTG	1 cut(s)	482
AfaI	GTAC	4 cut(s)	419, 1561, 2023, 2170
AfiI	CCNNNNNNNGG	9 cut(s)	85, 304, 721, 856, 857, 929, 1258, 2121, 2434
AflIII	ACRYGT	1 cut(s)	869
AgeI	ACCGGT	1 cut(s)	447
AgsI	TTSAA	3 cut(s)	1054, 1522, 2119
AjnI	CCWGG	7 cut(s)	219, 248, 312, 636, 1564, 1861, 2050
AloI	GAACNNNNNNTCC	1 cut(s)	28
AluBI	AGCT	8 cut(s)	303, 604, 957, 1192, 1415, 1474, 1594, 2444
AluI	AGCT	8 cut(s)	303, 604, 957, 1192, 1415, 1474, 1594, 2444
Alw21I	GWGCWC	5 cut(s)	844, 1246, 1890, 2063, 2081
Alw26I	GTCTC	7 cut(s)	234, 432, 1001, 1107, 1860, 2033, 2477
Alw44I	GTGCAC	1 cut(s)	840
AlwNI	CAGNNNCTG	1 cut(s)	2312
Ama87I	CYCGRG	1 cut(s)	467
Aor13HI	TCCGGA	3 cut(s)	47, 53, 59
AoxI	GGCC	9 cut(s)	306, 315, 530, 550, 581, 740, 1459, 1615, 2394
ApaI	GGGCCC	2 cut(s)	310, 585
ApaLI	GTGCAC	1 cut(s)	840
ApeKI	GCWGC	8 cut(s)	618, 717, 1041, 1143, 1628, 1765, 2150, 2444
ApoI	RAATTY	3 cut(s)	664, 1511, 2388
AsiGI	ACCGGT	1 cut(s)	447
AspLEI	GCGC	5 cut(s)	200, 334, 341, 351, 1825
AsuC2I	CCSGG	3 cut(s)	237, 579, 977
AsuHPI	GGTGA	8 cut(s)	118, 527, 704, 772, 980, 1273, 2058, 2308
AvaI	CYCGRG	1 cut(s)	467
AvaII	GGWCC	5 cut(s)	88, 252, 1379, 1487, 1921
BaeGI	GKGCMC	5 cut(s)	310, 585, 844, 851, 977
BalI	TGGCCA	1 cut(s)	1461
BamHI	GGATCC	4 cut(s)	50, 56, 62, 228
BanI	GGYRCC	5 cut(s)	776, 972, 1283, 1479, 1822
BanII	GRGCYC	3 cut(s)	310, 585, 1998
BauI	CACGAG	2 cut(s)	605, 734
BbsI	GAAGAC	2 cut(s)	271, 2530
Bbv12I	GWGCWC	5 cut(s)	844, 1246, 1890, 2063, 2081
BbvCI	CCTCAGC	1 cut(s)	139
BbvI	GCAGC	8 cut(s)	605, 704, 1028, 1130, 1640, 1777, 2162, 2431
BccI	CCATC	7 cut(s)	582, 1145, 1225, 1252, 1312, 1636, 1765
BceAI	ACGGC	1 cut(s)	736
BcgI	CGANNNNNNTGC	4 cut(s)	1870, 1904, 2355, 2389
BciT130I	CCWGG	7 cut(s)	221, 250, 314, 638, 1566, 1863, 2052
BcnI	CCSGG	3 cut(s)	237, 579, 977
BcoDI	GTCTC	7 cut(s)	234, 432, 1001, 1107, 1860, 2033, 2477
BfaI	CTAG	1 cut(s)	2417
BfmI	CTRYAG	2 cut(s)	2445, 2464
BfoI	RGCGCY	3 cut(s)	201, 335, 1826
BglI	GCCNNNNNGGC	2 cut(s)	314, 1762
Bme18I	GGWCC	5 cut(s)	88, 252, 1379, 1487, 1921
BmeT110I	CYCGRG	1 cut(s)	467
BmrI	ACTGGG	2 cut(s)	418, 1247
BmsI	GCATC	5 cut(s)	303, 350, 904, 1135, 2096
BmuI	ACTGGG	2 cut(s)	418, 1247
BpiI	GAAGAC	2 cut(s)	271, 2530
BplI	GAGNNNNNCTC	2 cut(s)	2063, 2095
BpmI	CTGGAG	2 cut(s)	242, 1884
Bpu10I	CCTNAGC	2 cut(s)	139, 1475
BpuEI	CTTGAG	2 cut(s)	1025, 1724
BpuMI	CCSGG	3 cut(s)	237, 579, 977
BsaBI	GATNNNNATC	4 cut(s)	27, 33, 55, 61
BsaHI	GRCGYC	2 cut(s)	182, 1823
BsaI	GGTCTC	2 cut(s)	234, 2477
BsaJI	CCNNGG	5 cut(s)	491, 578, 991, 1483, 1917
BsaWI	WCCGGW	5 cut(s)	47, 53, 59, 128, 447
BsaXI	ACNNNNNCTCC	7 cut(s)	26, 793, 823, 1594, 1624, 2196, 2226
Bsc4I	CCNNNNNNNGG	9 cut(s)	85, 304, 721, 856, 857, 929, 1258, 2121, 2434
Bse118I	RCCGGY	1 cut(s)	447
Bse1I	ACTGG	2 cut(s)	424, 1253
Bse3DI	GCAATG	2 cut(s)	556, 2167
Bse8I	GATNNNNATC	4 cut(s)	27, 33, 55, 61
BseAI	TCCGGA	3 cut(s)	47, 53, 59
BseBI	CCWGG	7 cut(s)	221, 250, 314, 638, 1566, 1863, 2052
BseDI	CCNNGG	5 cut(s)	491, 578, 991, 1483, 1917
BseJI	GATNNNNATC	4 cut(s)	27, 33, 55, 61
BseLI	CCNNNNNNNGG	9 cut(s)	85, 304, 721, 856, 857, 929, 1258, 2121, 2434
BseMI	GCAATG	2 cut(s)	556, 2167
BseMII	CTCAG	6 cut(s)	153, 1185, 1305, 1545, 2061, 2499
BseNI	ACTGG	2 cut(s)	424, 1253
BseRI	GAGGAG	6 cut(s)	811, 814, 1323, 1488, 2204, 2465
BseSI	GKGCMC	5 cut(s)	310, 585, 844, 851, 977
BseXI	GCAGC	8 cut(s)	605, 704, 1028, 1130, 1640, 1777, 2162, 2431
BseYI	CCCAGC	1 cut(s)	303
BsgI	GTGCAG	3 cut(s)	241, 2165, 2307
Bsh1236I	CGCG	3 cut(s)	339, 349, 993
BshFI	GGCC	9 cut(s)	308, 317, 532, 552, 583, 742, 1461, 1617, 2396
BshNI	GGYRCC	5 cut(s)	776, 972, 1283, 1479, 1822
BshTI	ACCGGT	1 cut(s)	447
BsiHKAI	GWGCWC	5 cut(s)	844, 1246, 1890, 2063, 2081
BsiHKCI	CYCGRG	1 cut(s)	467
BsiSI	CCGG	9 cut(s)	48, 54, 60, 129, 195, 237, 448, 578, 977
BslFI	GGGAC	1 cut(s)	1169
BslI	CCNNNNNNNGG	9 cut(s)	85, 304, 721, 856, 857, 929, 1258, 2121, 2434
BsmAI	GTCTC	7 cut(s)	234, 432, 1001, 1107, 1860, 2033, 2477
BsmFI	GGGAC	1 cut(s)	1169
BsmI	GAATGC	1 cut(s)	2269
BsnI	GGCC	9 cut(s)	308, 317, 532, 552, 583, 742, 1461, 1617, 2396
Bso31I	GGTCTC	2 cut(s)	234, 2477
BsoBI	CYCGRG	1 cut(s)	467
Bsp120I	GGGCCC	2 cut(s)	306, 581
Bsp13I	TCCGGA	3 cut(s)	47, 53, 59
Bsp1407I	TGTACA	1 cut(s)	2021
Bsp19I	CCATGG	2 cut(s)	1483, 1917
BspANI	GGCC	9 cut(s)	308, 317, 532, 552, 583, 742, 1461, 1617, 2396
BspCNI	CTCAG	6 cut(s)	152, 1184, 1306, 1546, 2062, 2498
BspEI	TCCGGA	3 cut(s)	47, 53, 59
BspFNI	CGCG	3 cut(s)	339, 349, 993
BspHI	TCATGA	1 cut(s)	2137
BspMAI	CTGCAG	1 cut(s)	2449
BspT107I	GGYRCC	5 cut(s)	776, 972, 1283, 1479, 1822
BspTNI	GGTCTC	2 cut(s)	234, 2477
BsrBI	CCGCTC	2 cut(s)	226, 1738
BsrDI	GCAATG	2 cut(s)	556, 2167
BsrFI	RCCGGY	1 cut(s)	447
BsrGI	TGTACA	1 cut(s)	2021
BsrI	ACTGG	2 cut(s)	424, 1253
BssAI	RCCGGY	1 cut(s)	447
BssECI	CCNNGG	5 cut(s)	491, 578, 991, 1483, 1917
BssNAI	GTATAC	1 cut(s)	1835
BssNI	GRCGYC	2 cut(s)	182, 1823
BssSI	CACGAG	2 cut(s)	605, 734
BssT1I	CCWWGG	2 cut(s)	1483, 1917
Bst1107I	GTATAC	1 cut(s)	1835
Bst2BI	CACGAG	2 cut(s)	605, 734
Bst2UI	CCWGG	7 cut(s)	221, 250, 314, 638, 1566, 1863, 2052
Bst4CI	ACNGT	5 cut(s)	846, 1021, 1118, 1910, 2095
Bst6I	CTCTTC	5 cut(s)	293, 784, 857, 878, 2502
BstACI	GRCGYC	2 cut(s)	182, 1823
BstAUI	TGTACA	1 cut(s)	2021
BstDSI	CCRYGG	4 cut(s)	491, 991, 1483, 1917
BstEII	GGTNACC	1 cut(s)	1706
BstENI	CCTNNNNNAGG	1 cut(s)	2119
BstFNI	CGCG	3 cut(s)	339, 349, 993
BstH2I	RGCGCY	3 cut(s)	201, 335, 1826
BstHHI	GCGC	5 cut(s)	200, 334, 341, 351, 1825
BstMAI	GTCTC	7 cut(s)	234, 432, 1001, 1107, 1860, 2033, 2477
BstNI	CCWGG	7 cut(s)	221, 250, 314, 638, 1566, 1863, 2052
BstNSI	RCATGY	4 cut(s)	873, 1089, 1275, 1833
BstPI	GGTNACC	1 cut(s)	1706
BstSFI	CTRYAG	2 cut(s)	2445, 2464
BstSLI	GKGCMC	5 cut(s)	310, 585, 844, 851, 977
BstUI	CGCG	3 cut(s)	339, 349, 993
BstV1I	GCAGC	8 cut(s)	605, 704, 1028, 1130, 1640, 1777, 2162, 2431
BstV2I	GAAGAC	2 cut(s)	271, 2530
BstX2I	RGATCY	5 cut(s)	50, 56, 62, 165, 228
BstYI	RGATCY	5 cut(s)	50, 56, 62, 165, 228
BstZ17I	GTATAC	1 cut(s)	1835
BsuRI	GGCC	9 cut(s)	308, 317, 532, 552, 583, 742, 1461, 1617, 2396
BtgI	CCRYGG	4 cut(s)	491, 991, 1483, 1917
BtsI	GCAGTG	3 cut(s)	924, 1004, 2378
BtsIMutI	CAGTG	7 cut(s)	924, 1004, 1260, 1311, 1906, 2063, 2378
CaiI	CAGNNNCTG	1 cut(s)	2312
CciI	TCATGA	1 cut(s)	2137
CfoI	GCGC	5 cut(s)	200, 334, 341, 351, 1825
Cfr10I	RCCGGY	1 cut(s)	447
Cfr42I	CCGCGG	1 cut(s)	994
CsiI	ACCWGGT	1 cut(s)	248
Csp6I	GTAC	4 cut(s)	418, 1560, 2022, 2169
CspAI	ACCGGT	1 cut(s)	447
CspCI	CAANNNNNGTGG	2 cut(s)	1276, 1311
CviQI	GTAC	4 cut(s)	418, 1560, 2022, 2169
DinI	GGCGCC	1 cut(s)	1824
DraIII	CACNNNGTG	1 cut(s)	482
EaeI	YGGCCR	2 cut(s)	550, 1459
Eam1104I	CTCTTC	5 cut(s)	293, 784, 857, 878, 2502
EarI	CTCTTC	5 cut(s)	293, 784, 857, 878, 2502
EciI	GGCGGA	1 cut(s)	392
Eco130I	CCWWGG	2 cut(s)	1483, 1917
Eco147I	AGGCCT	2 cut(s)	317, 532
Eco24I	GRGCYC	3 cut(s)	310, 585, 1998
Eco31I	GGTCTC	2 cut(s)	234, 2477
Eco47I	GGWCC	5 cut(s)	88, 252, 1379, 1487, 1921
Eco57I	CTGAAG	1 cut(s)	557
Eco88I	CYCGRG	1 cut(s)	467
Eco91I	GGTNACC	1 cut(s)	1706
EcoNI	CCTNNNNNAGG	1 cut(s)	2119
EcoO109I	RGGNCCY	3 cut(s)	581, 582, 740
EcoO65I	GGTNACC	1 cut(s)	1706
EcoRII	CCWGG	7 cut(s)	219, 248, 312, 636, 1564, 1861, 2050
EcoT14I	CCWWGG	2 cut(s)	1483, 1917
EcoT38I	GRGCYC	3 cut(s)	310, 585, 1998
EgeI	GGCGCC	1 cut(s)	1824
EheI	GGCGCC	1 cut(s)	1824
ErhI	CCWWGG	2 cut(s)	1483, 1917
FalI	AAGNNNNNCTT	2 cut(s)	1768, 1800
FaqI	GGGAC	1 cut(s)	1169
FauI	CCCGC	6 cut(s)	83, 317, 756, 986, 1037, 1743
FblI	GTMKAC	1 cut(s)	1834
FriOI	GRGCYC	3 cut(s)	310, 585, 1998
FspBI	CTAG	1 cut(s)	2417
GlaI	GCGC	5 cut(s)	199, 333, 340, 350, 1824
GsaI	CCCAGC	1 cut(s)	307
GsuI	CTGGAG	2 cut(s)	242, 1884
HaeII	RGCGCY	3 cut(s)	201, 335, 1826
HaeIII	GGCC	9 cut(s)	308, 317, 532, 552, 583, 742, 1461, 1617, 2396
HapII	CCGG	9 cut(s)	48, 54, 60, 129, 195, 237, 448, 578, 977
HhaI	GCGC	5 cut(s)	200, 334, 341, 351, 1825
Hin1I	GRCGYC	2 cut(s)	182, 1823
Hin6I	GCGC	5 cut(s)	198, 332, 339, 349, 1823
HinP1I	GCGC	5 cut(s)	198, 332, 339, 349, 1823
HindIII	AAGCTT	1 cut(s)	1190
HpaII	CCGG	9 cut(s)	48, 54, 60, 129, 195, 237, 448, 578, 977
HphI	GGTGA	8 cut(s)	118, 527, 704, 772, 980, 1273, 2058, 2308
Hpy99I	CGWCG	1 cut(s)	2547
HpyAV	CCTTC	6 cut(s)	184, 604, 890, 1051, 2293, 2438
HpyCH4III	ACNGT	5 cut(s)	846, 1021, 1118, 1910, 2095
HpyCH4IV	ACGT	1 cut(s)	182
HpySE526I	ACGT	1 cut(s)	182
Hsp92I	GRCGYC	2 cut(s)	182, 1823
HspAI	GCGC	5 cut(s)	198, 332, 339, 349, 1823
KasI	GGCGCC	1 cut(s)	1822
Kpn2I	TCCGGA	3 cut(s)	47, 53, 59
KspI	CCGCGG	1 cut(s)	994
LmnI	GCTCC	5 cut(s)	223, 1249, 1412, 1850, 1966
Lsp1109I	GCAGC	8 cut(s)	605, 704, 1028, 1130, 1640, 1777, 2162, 2431
LweI	GCATC	5 cut(s)	303, 350, 904, 1135, 2096
MabI	ACCWGGT	1 cut(s)	248
MaeI	CTAG	1 cut(s)	2417
MaeII	ACGT	1 cut(s)	182
MaeIII	GTNAC	8 cut(s)	384, 674, 986, 1024, 1261, 1706, 1904, 2089
MbiI	CCGCTC	2 cut(s)	226, 1738
MfeI	CAATTG	2 cut(s)	544, 1266
MflI	RGATCY	5 cut(s)	50, 56, 62, 165, 228
MlsI	TGGCCA	1 cut(s)	1461
MluCI	AATT	9 cut(s)	394, 544, 664, 695, 1266, 1511, 2198, 2264, 2388
MluNI	TGGCCA	1 cut(s)	1461
Mly113I	GGCGCC	1 cut(s)	1823
MlyI	GAGTC	4 cut(s)	5, 92, 2205, 2219
MmeI	TCCRAC	2 cut(s)	256, 345
Mox20I	TGGCCA	1 cut(s)	1461
MroI	TCCGGA	3 cut(s)	47, 53, 59
MscI	TGGCCA	1 cut(s)	1461
MseI	TTAA	4 cut(s)	660, 953, 1109, 1893
MslI	CAYNNNNRTG	4 cut(s)	874, 1233, 1276, 1316
Msp20I	TGGCCA	1 cut(s)	1461
MspA1I	CMGCKG	5 cut(s)	717, 925, 993, 1143, 1976
MspI	CCGG	9 cut(s)	48, 54, 60, 129, 195, 237, 448, 578, 977
MunI	CAATTG	2 cut(s)	544, 1266
Mva1269I	GAATGC	1 cut(s)	2269
MvaI	CCWGG	7 cut(s)	221, 250, 314, 638, 1566, 1863, 2052
MvnI	CGCG	3 cut(s)	339, 349, 993
NarI	GGCGCC	1 cut(s)	1823
NciI	CCSGG	3 cut(s)	237, 579, 977
NcoI	CCATGG	2 cut(s)	1483, 1917
NmuCI	GTSAC	5 cut(s)	384, 986, 1024, 1261, 1904
NspI	RCATGY	4 cut(s)	873, 1089, 1275, 1833
PaeR7I	CTCGAG	1 cut(s)	467
PagI	TCATGA	1 cut(s)	2137
PceI	AGGCCT	2 cut(s)	317, 532
PciI	ACATGT	1 cut(s)	869
PcsI	WCGNNNNNNNCGW	1 cut(s)	353
PctI	GAATGC	1 cut(s)	2269
PfeI	GAWTC	9 cut(s)	17, 23, 472, 496, 1690, 1744, 2194, 2258, 2534
PflFI	GACNNNGTC	1 cut(s)	250
PfoI	TCCNGGA	2 cut(s)	636, 1861
PinAI	ACCGGT	1 cut(s)	447
PleI	GAGTC	4 cut(s)	5, 92, 2205, 2219
PluTI	GGCGCC	1 cut(s)	1826
PpsI	GAGTC	4 cut(s)	5, 92, 2205, 2219
PscI	ACATGT	1 cut(s)	869
Psp6I	CCWGG	7 cut(s)	219, 248, 312, 636, 1564, 1861, 2050
PspEI	GGTNACC	1 cut(s)	1706
PspFI	CCCAGC	1 cut(s)	303
PspGI	CCWGG	7 cut(s)	219, 248, 312, 636, 1564, 1861, 2050
PspOMI	GGGCCC	2 cut(s)	306, 581
PsrI	GAACNNNNNNTAC	2 cut(s)	1552, 1584
PstI	CTGCAG	1 cut(s)	2449
PstNI	CAGNNNCTG	1 cut(s)	2312
PsuI	RGATCY	5 cut(s)	50, 56, 62, 165, 228
PsyI	GACNNNGTC	1 cut(s)	250
RsaI	GTAC	4 cut(s)	419, 1561, 2023, 2170
RsaNI	GTAC	4 cut(s)	418, 1560, 2022, 2169
RseI	CAYNNNNRTG	4 cut(s)	874, 1233, 1276, 1316
SacII	CCGCGG	1 cut(s)	994
SaqAI	TTAA	4 cut(s)	660, 953, 1109, 1893
SchI	GAGTC	4 cut(s)	5, 92, 2205, 2219
SexAI	ACCWGGT	1 cut(s)	248
SfaNI	GCATC	5 cut(s)	303, 350, 904, 1135, 2096
SfcI	CTRYAG	2 cut(s)	2445, 2464
SfiI	GGCCNNNNNGGCC	1 cut(s)	314
SfoI	GGCGCC	1 cut(s)	1824
Sfr274I	CTCGAG	1 cut(s)	467
Sfr303I	CCGCGG	1 cut(s)	994
SgrBI	CCGCGG	1 cut(s)	994
SinI	GGWCC	5 cut(s)	88, 252, 1379, 1487, 1921
SlaI	CTCGAG	1 cut(s)	467
SmiMI	CAYNNNNRTG	4 cut(s)	874, 1233, 1276, 1316
SmlI	CTYRAG	3 cut(s)	467, 1004, 1739
SmoI	CTYRAG	3 cut(s)	467, 1004, 1739
Sse9I	AATT	9 cut(s)	394, 544, 664, 695, 1266, 1511, 2198, 2264, 2388
SseBI	AGGCCT	2 cut(s)	317, 532
SspDI	GGCGCC	1 cut(s)	1822
SspMI	CTAG	1 cut(s)	2417
StuI	AGGCCT	2 cut(s)	317, 532
StyI	CCWWGG	2 cut(s)	1483, 1917
TaaI	ACNGT	5 cut(s)	846, 1021, 1118, 1910, 2095
TaiI	ACGT	1 cut(s)	185
TaqI	TCGA	4 cut(s)	468, 1336, 2375, 2542
TasI	AATT	9 cut(s)	394, 544, 664, 695, 1266, 1511, 2198, 2264, 2388
TatI	WGTACW	3 cut(s)	417, 1559, 2021
TauI	GCSGC	7 cut(s)	81, 377, 552, 925, 1126, 1143, 1396
TfiI	GAWTC	9 cut(s)	17, 23, 472, 496, 1690, 1744, 2194, 2258, 2534
Tru1I	TTAA	4 cut(s)	660, 953, 1109, 1893
Tru9I	TTAA	4 cut(s)	660, 953, 1109, 1893
TscAI	CASTG	7 cut(s)	924, 1004, 1260, 1318, 1913, 2063, 2385
TseFI	GTSAC	5 cut(s)	384, 986, 1024, 1261, 1904
TseI	GCWGC	8 cut(s)	618, 717, 1041, 1143, 1628, 1765, 2150, 2444
Tsp45I	GTSAC	5 cut(s)	384, 986, 1024, 1261, 1904
TspDTI	ATGAA	6 cut(s)	17, 909, 1535, 2417, 2510, 2514
TspGWI	ACGGA	3 cut(s)	101, 253, 265
TspRI	CASTG	7 cut(s)	924, 1004, 1260, 1318, 1913, 2063, 2385
Tth111I	GACNNNGTC	1 cut(s)	250
VneI	GTGCAC	1 cut(s)	840
VpaK11BI	GGWCC	5 cut(s)	88, 252, 1379, 1487, 1921
XagI	CCTNNNNNAGG	1 cut(s)	2119
XapI	RAATTY	3 cut(s)	664, 1511, 2388
XceI	RCATGY	4 cut(s)	873, 1089, 1275, 1833
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1596
XhoI	CTCGAG	1 cut(s)	467
XmiI	GTMKAC	1 cut(s)	1834
XspI	CTAG	1 cut(s)	2417
ZraI	GACGTC	1 cut(s)	183

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.