Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0005198

Physical Location & Seq

Genomic Coordinates Reverse (-)

33439790 .. 33444768

Gene Structure 4979 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1266200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2427 bp

ATGAGTTTGGGAGGTTTGATCAGTAGCAGCGGGGGTGGAAATAGTGGACGTGGGAGAGGAAGAGTTGTGGCTGAAATTGCTCCACATGCCCCTTCCTTCATGCCTAGTGGCACCATTGCACACGCACACTTTCTCTCTTCCCCAATCCCCAATCCCAGGCAGACTTCCTCCTCTGCCCTCTCTCTCTCCATTCAGAAGATGGACGGTCACGGTGAACTTGGTTTCGCCGAGAATTTCGACCCCGGGATCATCGGAAGGCTGAGGGATGATGAGTACGAGAGCAAGTCGGGGAGTGATAACTTTGAGGGCGCATCTGGGGATGATCAGGACGGCGGCGGAGGCGACCAACGGCCGAGGAAGAAGAAGTACCTTAGGCACACTCCTGACCAAATCCAAGAACTTGAAAATTTTTTCAAAGAGTGTCCTCATCCGGATGAGAAGCAAAGGTCGGAGCTCGGCCGCCGTCTCGGCTTAGAGTCGAAGCAAGTCAAGTTCTGGTTTCAAAACAGAAGAACTCATATGAAGACACAACTCGAACGCCACGAGAATATAATTCTCAGGCAAGAGAATGACAAGCTGATAGCTGAGAATGGTGTGATGAAGGATGCTATTTCAAACCCAGTTTGCAACAATTGTGGTGGTCCGGCCATTCCTGGTCAAATATCGTTTGATGAACACCAGCTTAGGATTGAGAATGCTCGATTGAAGGAAGAACTGAACCGGATATGTGCTCTGGCGAACAAGTTGCCCATCTCCTCCTTGGGCATTCCTGGCTCTATTCCGAATGCAACCTCTGGTTTGGAACTTGGGGTTGGAAGGAATGGGATGGATGGTATGAATGCTGTAAGCGCTGGGTTGCCAATGGGGCTTGATTTAGGAGATGGGGTTTCAAGCTCTTCCCTTATGATCCCTCTCGTTAAGACCTCATCAGGCATGATGGGGAATGAGGTACCATTTGAGAGATCTATGTACATAGATCTTGCATTGGCAGCTATGGATGAATTGGTGAAGATGGTTCAGGTGGATAGCCCCCTGTGGATCAAAAGCTCAGATGGGACAGATATATTGAACATTGAGGAGTACCAGGCATTTTCTTGCATTGGTATGAAGCCTAGCAGCCTTGTAACTGAAGCCACAAGGGACACTTGTGTCGTAATAGTAAACAGCTTGGCTCTCGTTGAGATATTGATGGATGCGAATCGATGGGCAGAAATGTTTCAGTGTTTGGTCGCTCGAGCCTCCACCATTGATATGATATCCAACGGCATGGATGGGACCAGAAATGGTGCTCTTCAACTGATGTATGCTGAGCTTCAAATGCTATCCCCTTTAGTCCCTGTCCGTCCGCTGAAGTTCCTCCGTTTTTGCAAGCAACATGCAGAGGGTGTATGGGCTGTGGTTGATGTTTCCATTGACATCAATCAAGAAGGTCCCAACACAAACCCGTTTGTGGACTGCCGGAGGCTTCCTTCCGGATGCATTGTACAAGATATGCCTAACAATTGCTCCAAGGTAACATGGATTGAGCACACTGAATATGATGAGAATATTGTCCACCACCTCTTCCGGGAATCACTGCGTTCGGGTATGGGCTTTGGTGCACAGAGATGGCTTGCCACTCTGCAAAGGCAATGTGAGAGCCTGGCATTCCTCATTTCGTCAGCCAACTCCAATGAAGATCTCTCAGGGATAGAGGTCAGCGGTAAGAAAAGCATGCTAAAGCTTGCACAGCGTATGGTGGATAACTTCTGTGCTGGGATTTCTGCCTCGTCTGTGCGCAAGTGGGACAAACTTTGCTTTGAAAATGTTAGTGAAGATGTGAGGGTTATGGCCCGGAAGAGTATGAATGACCCCGGCGAGCCACCAGGGATTGTGTTGAGTGTCACAACAACTGTTTGGGTTCCGGTATCAAGGCATAGGGTGTTTGAGTTTTTGCGGGATGAACAGTTTAGGAAGGACTGGGACATTCTGTCCAGCGGTGTTCCAATGCAACGCATTGTCCAAATTGGCAAGGGCCAAGGAGGTGCCAATAGTGTTTCTCTTCTTCGTGCTAACGCGCCAGATGCCAACAACAGTAACGTGCTGATATTGCAAGAGACGTGGACTGACCCTACAGGCTCACTTGTAGTGTATGCACCGGTTGACACAGAATCAATAAATGTCGTGATGCGCGGTGGAGATTCTGCCTATGTAGCTCTTCTGCCATCAGGGTTTGCAATCCTTCCTGGTGGCCCGCCTGGCTACGGAGTGGTCAAATCTGAAGGCAATGGCTATGGTGGCGGTGGCGGATGCTTGCTGACTGTTGCATTCCAAATTCTGGTTAGCAGCCTTCCTACAGCCAAAATCAACATGGAGTCGGTTGAGACAGTTAGCACACTCATATCTTGCACCATTCAGAAAATAAAATCGTCTCTTCTAGCAGCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Homeobox START

Protein Analysis

809

Amino Acids

87.73

Weight (kDa)

5.56

Isoelectric Point (pI)

43.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Homeodomain	PF00046	120 - 175	3.9e-19	Homeodomain
START	PF01852	327 - 547	2.6e-47	START domain
PDF2_C	PF25797	566 - 805	9.1e-92	PDF2-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005198)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g29130 FvH4_7g29130 FvH4_7g29130 FvH4_7g29130 FvH4_7g29130 FvH4_7g29130 FvH4_7g29130 FvH4_7g29130
malus_domestica	MD01G1198100.v1.1 MD07G1266200.v1.1
prunus_persica	Prupe.2G291400_v2.0.a1
pyrus_communis	pycom01g20820 pycom07g24390
rosa_chinensis	RchiOBHm_Chr1g0376881
rosa_laevigata	RLG00000026562
rosa_multiflora	Rmu_co8045660.1_g000001 Rmu_sc0002113.1_g000002 Rmu_ssc0000125.1_g000012
rosa_roxburghii	Rroxscaffold_4G00281650
rosa_rugosa	Rorug01G0399300 Rorug01G0399400 Rorug01G0399500 Rorug01G0399600 Rorug01G0399600
rosa_samantha	Rh1AG416600 Rh1BG375800 Rh1CG389600 Rh1DG406400
rosa_wichuraiana	Rw1G036550 Rw3G023490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1148
Acc16I	TGCGCA	1 cut(s)	1778
Acc65I	GGTACC	1 cut(s)	949
AccB1I	GGYRCC	3 cut(s)	110, 949, 2024
AccB7I	CCANNNNNTGG	1 cut(s)	2317
AccII	CGCG	2 cut(s)	2057, 2172
AccIII	TCCGGA	2 cut(s)	430, 1472
AclWI	GGATC	3 cut(s)	254, 901, 1046
AcoI	YGGCCR	3 cut(s)	350, 457, 645
AcsI	RAATTY	3 cut(s)	232, 406, 2313
AcuI	CTGAAG	3 cut(s)	1149, 1370, 2280
AfaI	GTAC	6 cut(s)	275, 368, 951, 971, 1082, 1485
AfeI	AGCGCT	1 cut(s)	850
AfiI	CCNNNNNNNGG	5 cut(s)	156, 1450, 1567, 2243, 2317
AgeI	ACCGGT	1 cut(s)	2137
AhdI	GACNNNNNGTC	1 cut(s)	1969
AjiI	CACGTC	2 cut(s)	50, 2100
AjnI	CCWGG	8 cut(s)	155, 652, 769, 1083, 1641, 1864, 2224, 2236
AjuI	GAANNNNNNNTTGG	2 cut(s)	795, 827
Alw21I	GWGCWC	5 cut(s)	456, 733, 1291, 1530, 1603
Alw26I	GTCTC	4 cut(s)	470, 2090, 2357, 2415
Alw44I	GTGCAC	1 cut(s)	1599
AlwI	GGATC	3 cut(s)	254, 901, 1046
Ama87I	CYCGRG	2 cut(s)	242, 1233
Aor13HI	TCCGGA	2 cut(s)	430, 1472
Aor51HI	AGCGCT	1 cut(s)	850
AoxI	GGCC	6 cut(s)	350, 457, 645, 1830, 2014, 2230
ApaLI	GTGCAC	1 cut(s)	1599
ApeKI	GCWGC	5 cut(s)	27, 989, 1116, 2325, 2420
ApoI	RAATTY	3 cut(s)	232, 406, 2313
AsiGI	ACCGGT	1 cut(s)	2137
Asp700I	GAANNNNTTC	1 cut(s)	1215
Asp718I	GGTACC	1 cut(s)	949
AspLEI	GCGC	5 cut(s)	311, 851, 1779, 2059, 2172
AspS9I	GGNCC	6 cut(s)	641, 1275, 1430, 1831, 2014, 2231
AsuC2I	CCSGG	5 cut(s)	243, 244, 1568, 1834, 1854
AsuHPI	GGTGA	2 cut(s)	224, 1018
AvaI	CYCGRG	2 cut(s)	242, 1233
AvaII	GGWCC	3 cut(s)	641, 1275, 1430
AxyI	CCTNAGG	1 cut(s)	371
BaeGI	GKGCMC	1 cut(s)	1603
BanI	GGYRCC	3 cut(s)	110, 949, 2024
BanII	GRGCYC	1 cut(s)	456
BarI	GAAGNNNNNNTAC	2 cut(s)	350, 382
BauI	CACGAG	1 cut(s)	542
BbsI	GAAGAC	1 cut(s)	530
Bbv12I	GWGCWC	5 cut(s)	456, 733, 1291, 1530, 1603
BbvCI	CCTCAGC	1 cut(s)	260
BbvI	GCAGC	4 cut(s)	39, 1001, 1128, 2337
BceAI	ACGGC	4 cut(s)	346, 365, 447, 1279
BcgI	CGANNNNNNTGC	2 cut(s)	727, 761
BciT130I	CCWGG	8 cut(s)	157, 654, 771, 1085, 1643, 1866, 2226, 2238
BclI	TGATCA	2 cut(s)	18, 322
BcnI	CCSGG	5 cut(s)	243, 244, 1568, 1834, 1854
BcoDI	GTCTC	4 cut(s)	470, 2090, 2357, 2415
BfaI	CTAG	3 cut(s)	105, 1113, 2417
BfmI	CTRYAG	2 cut(s)	2112, 2334
BfoI	RGCGCY	1 cut(s)	852
BglI	GCCNNNNNGGC	3 cut(s)	468, 865, 2238
BglII	AGATCT	3 cut(s)	962, 976, 1678
BisI	GCNGC	7 cut(s)	28, 334, 460, 990, 1117, 2326, 2421
BlpI	GCTNAGC	1 cut(s)	1308
BlsI	GCNGC	7 cut(s)	29, 335, 461, 991, 1118, 2327, 2422
Bme18I	GGWCC	3 cut(s)	641, 1275, 1430
BmeRI	GACNNNNNGTC	1 cut(s)	1969
BmeT110I	CYCGRG	2 cut(s)	242, 1233
BmgBI	CACGTC	2 cut(s)	50, 2100
BmgT120I	GGNCC	6 cut(s)	641, 1275, 1430, 1831, 2014, 2231
BmiI	GGNNCC	6 cut(s)	112, 951, 1276, 1432, 1902, 2026
BmrI	ACTGGG	2 cut(s)	614, 1969
BmsI	GCATC	7 cut(s)	320, 595, 1183, 1466, 2053, 2157, 2279
BmuI	ACTGGG	2 cut(s)	614, 1969
BpiI	GAAGAC	1 cut(s)	530
Bpu10I	CCTNAGC	2 cut(s)	260, 683
Bpu1102I	GCTNAGC	1 cut(s)	1308
BpuMI	CCSGG	5 cut(s)	243, 244, 1568, 1834, 1854
Bsa29I	ATCGAT	1 cut(s)	1201
BsaBI	GATNNNNATC	1 cut(s)	1197
BsaJI	CCNNGG	9 cut(s)	155, 241, 242, 353, 759, 1509, 1852, 1865, 2017
BsaWI	WCCGGW	5 cut(s)	430, 720, 1472, 1903, 2137
BsaXI	ACNNNNNCTCC	4 cut(s)	870, 900, 1490, 1520
Bsc4I	CCNNNNNNNGG	5 cut(s)	156, 1450, 1567, 2243, 2317
Bse118I	RCCGGY	1 cut(s)	2137
Bse1I	ACTGG	2 cut(s)	620, 1964
Bse21I	CCTNAGG	1 cut(s)	371
Bse3DI	GCAATG	3 cut(s)	114, 1637, 2272
Bse8I	GATNNNNATC	1 cut(s)	1197
BseAI	TCCGGA	2 cut(s)	430, 1472
BseBI	CCWGG	8 cut(s)	157, 654, 771, 1085, 1643, 1866, 2226, 2238
BseCI	ATCGAT	1 cut(s)	1201
BseDI	CCNNGG	9 cut(s)	155, 241, 242, 353, 759, 1509, 1852, 1865, 2017
BseJI	GATNNNNATC	1 cut(s)	1197
BseLI	CCNNNNNNNGG	5 cut(s)	156, 1450, 1567, 2243, 2317
BseMI	GCAATG	3 cut(s)	114, 1637, 2272
BseMII	CTCAG	6 cut(s)	251, 571, 576, 1062, 1299, 1698
BseNI	ACTGG	2 cut(s)	620, 1964
BseRI	GAGGAG	3 cut(s)	160, 745, 1091
BseSI	GKGCMC	1 cut(s)	1603
BseX3I	CGGCCG	2 cut(s)	350, 457
BseXI	GCAGC	4 cut(s)	39, 1001, 1128, 2337
BseYI	CCCAGC	2 cut(s)	851, 1754
Bsh1236I	CGCG	2 cut(s)	2057, 2172
Bsh1285I	CGRYCG	2 cut(s)	353, 460
BshFI	GGCC	6 cut(s)	352, 459, 647, 1832, 2016, 2232
BshNI	GGYRCC	3 cut(s)	110, 949, 2024
BshTI	ACCGGT	1 cut(s)	2137
BshVI	ATCGAT	1 cut(s)	1201
BsiEI	CGRYCG	2 cut(s)	353, 460
BsiHKAI	GWGCWC	5 cut(s)	456, 733, 1291, 1530, 1603
BsiHKCI	CYCGRG	2 cut(s)	242, 1233
BslFI	GGGAC	7 cut(s)	1069, 1154, 1288, 1319, 1416, 1799, 1976
BslI	CCNNNNNNNGG	5 cut(s)	156, 1450, 1567, 2243, 2317
BsmAI	GTCTC	4 cut(s)	470, 2090, 2357, 2415
BsmBI	CGTCTC	3 cut(s)	470, 2090, 2415
BsmFI	GGGAC	7 cut(s)	1069, 1154, 1288, 1319, 1416, 1799, 1976
BsmI	GAATGC	6 cut(s)	700, 765, 790, 844, 1646, 2306
BsnI	GGCC	6 cut(s)	352, 459, 647, 1832, 2016, 2232
BsoBI	CYCGRG	2 cut(s)	242, 1233
Bsp1286I	GDGCHC	5 cut(s)	456, 733, 1291, 1530, 1603
Bsp13I	TCCGGA	2 cut(s)	430, 1472
Bsp1407I	TGTACA	2 cut(s)	969, 1483
Bsp143I	GATC	8 cut(s)	18, 246, 322, 906, 962, 976, 1038, 1678
Bsp1720I	GCTNAGC	1 cut(s)	1308
BspANI	GGCC	6 cut(s)	352, 459, 647, 1832, 2016, 2232
BspCNI	CTCAG	6 cut(s)	252, 570, 577, 1061, 1300, 1697
BspDI	ATCGAT	1 cut(s)	1201
BspEI	TCCGGA	2 cut(s)	430, 1472
BspFNI	CGCG	2 cut(s)	2057, 2172
BspLI	GGNNCC	6 cut(s)	112, 951, 1276, 1432, 1902, 2026
BspPI	GGATC	3 cut(s)	254, 901, 1046
BspQI	GCTCTTC	3 cut(s)	901, 1296, 2202
BspT107I	GGYRCC	3 cut(s)	110, 949, 2024
BsrDI	GCAATG	3 cut(s)	114, 1637, 2272
BsrFI	RCCGGY	1 cut(s)	2137
BsrGI	TGTACA	2 cut(s)	969, 1483
BsrI	ACTGG	2 cut(s)	620, 1964
BssAI	RCCGGY	1 cut(s)	2137
BssECI	CCNNGG	9 cut(s)	155, 241, 242, 353, 759, 1509, 1852, 1865, 2017
BssMI	GATC	8 cut(s)	18, 246, 322, 906, 962, 976, 1038, 1678
BssSI	CACGAG	1 cut(s)	542
BssT1I	CCWWGG	3 cut(s)	759, 1509, 2017
Bst2BI	CACGAG	1 cut(s)	542
Bst2UI	CCWGG	8 cut(s)	157, 654, 771, 1085, 1643, 1866, 2226, 2238
Bst4CI	ACNGT	7 cut(s)	206, 212, 1894, 1947, 2075, 2302, 2368
Bst6I	CTCTTC	9 cut(s)	55, 142, 901, 1296, 1568, 1832, 2046, 2202, 2418
BstAUI	TGTACA	2 cut(s)	969, 1483
BstC8I	GCNNGC	7 cut(s)	1370, 1614, 1715, 1725, 1859, 2234, 2294
BstDEI	CTNAG	9 cut(s)	260, 371, 472, 557, 585, 683, 1048, 1308, 1684
BstFNI	CGCG	2 cut(s)	2057, 2172
BstH2I	RGCGCY	1 cut(s)	852
BstHHI	GCGC	5 cut(s)	311, 851, 1779, 2059, 2172
BstKTI	GATC	8 cut(s)	21, 249, 325, 909, 965, 979, 1041, 1681
BstMAI	GTCTC	4 cut(s)	470, 2090, 2357, 2415
BstMBI	GATC	8 cut(s)	18, 246, 322, 906, 962, 976, 1038, 1678
BstMCI	CGRYCG	2 cut(s)	353, 460
BstNI	CCWGG	8 cut(s)	157, 654, 771, 1085, 1643, 1866, 2226, 2238
BstNSI	RCATGY	3 cut(s)	89, 1379, 1717
BstSFI	CTRYAG	2 cut(s)	2112, 2334
BstSLI	GKGCMC	1 cut(s)	1603
BstUI	CGCG	2 cut(s)	2057, 2172
BstV1I	GCAGC	4 cut(s)	39, 1001, 1128, 2337
BstV2I	GAAGAC	1 cut(s)	530
BstX2I	RGATCY	3 cut(s)	962, 976, 1678
BstXI	CCANNNNNNTGG	1 cut(s)	1267
BstYI	RGATCY	3 cut(s)	962, 976, 1678
BstZI	CGGCCG	2 cut(s)	350, 457
Bsu15I	ATCGAT	1 cut(s)	1201
Bsu36I	CCTNAGG	1 cut(s)	371
BsuRI	GGCC	6 cut(s)	352, 459, 647, 1832, 2016, 2232
BsuTUI	ATCGAT	1 cut(s)	1201
BtrI	CACGTC	2 cut(s)	50, 2100
BtsI	GCAGTG	1 cut(s)	1574
BtsIMutI	CAGTG	3 cut(s)	1226, 1530, 1574
Cac8I	GCNNGC	7 cut(s)	1370, 1614, 1715, 1725, 1859, 2234, 2294
CfoI	GCGC	5 cut(s)	311, 851, 1779, 2059, 2172
Cfr10I	RCCGGY	1 cut(s)	2137
Cfr13I	GGNCC	6 cut(s)	641, 1275, 1430, 1831, 2014, 2231
Cfr9I	CCCGGG	1 cut(s)	242
ClaI	ATCGAT	1 cut(s)	1201
Csp6I	GTAC	6 cut(s)	274, 367, 950, 970, 1081, 1484
CspAI	ACCGGT	1 cut(s)	2137
CspCI	CAANNNNNGTGG	6 cut(s)	616, 619, 651, 654, 2081, 2116
CviAII	CATG	8 cut(s)	86, 100, 934, 1267, 1376, 1518, 1714, 2350
CviQI	GTAC	6 cut(s)	274, 367, 950, 970, 1081, 1484
DdeI	CTNAG	9 cut(s)	260, 371, 472, 557, 585, 683, 1048, 1308, 1684
DpnI	GATC	8 cut(s)	20, 248, 324, 908, 964, 978, 1040, 1680
DpnII	GATC	8 cut(s)	18, 246, 322, 906, 962, 976, 1038, 1678
DrdI	GACNNNNNNGTC	1 cut(s)	1148
DriI	GACNNNNNGTC	1 cut(s)	1969
DseDI	GACNNNNNNGTC	1 cut(s)	1148
EaeI	YGGCCR	3 cut(s)	350, 457, 645
EagI	CGGCCG	2 cut(s)	350, 457
Eam1104I	CTCTTC	9 cut(s)	55, 142, 901, 1296, 1568, 1832, 2046, 2202, 2418
Eam1105I	GACNNNNNGTC	1 cut(s)	1969
EarI	CTCTTC	9 cut(s)	55, 142, 901, 1296, 1568, 1832, 2046, 2202, 2418
EciI	GGCGGA	2 cut(s)	351, 2301
Ecl136II	GAGCTC	1 cut(s)	454
EclXI	CGGCCG	2 cut(s)	350, 457
Eco130I	CCWWGG	3 cut(s)	759, 1509, 2017
Eco24I	GRGCYC	1 cut(s)	456
Eco32I	GATATC	1 cut(s)	1257
Eco47I	GGWCC	3 cut(s)	641, 1275, 1430
Eco47III	AGCGCT	1 cut(s)	850
Eco52I	CGGCCG	2 cut(s)	350, 457
Eco53kI	GAGCTC	1 cut(s)	454
Eco57I	CTGAAG	3 cut(s)	1149, 1370, 2280
Eco81I	CCTNAGG	1 cut(s)	371
Eco88I	CYCGRG	2 cut(s)	242, 1233
EcoICRI	GAGCTC	1 cut(s)	454
EcoO109I	RGGNCCY	1 cut(s)	1430
EcoRII	CCWGG	8 cut(s)	155, 652, 769, 1083, 1641, 1864, 2224, 2236
EcoRV	GATATC	1 cut(s)	1257
EcoT14I	CCWWGG	3 cut(s)	759, 1509, 2017
EcoT22I	ATGCAT	1 cut(s)	1481
EcoT38I	GRGCYC	1 cut(s)	456
ErhI	CCWWGG	3 cut(s)	759, 1509, 2017
Esp3I	CGTCTC	3 cut(s)	470, 2090, 2415
FaeI	CATG	8 cut(s)	89, 103, 937, 1270, 1379, 1521, 1717, 2353
FalI	AAGNNNNNCTT	2 cut(s)	1129, 1161
FaqI	GGGAC	7 cut(s)	1069, 1154, 1288, 1319, 1416, 1799, 1976
FatI	CATG	8 cut(s)	85, 99, 933, 1266, 1375, 1517, 1713, 2349
FauI	CCCGC	3 cut(s)	23, 1929, 2241
FauNDI	CATATG	1 cut(s)	519
FbaI	TGATCA	2 cut(s)	18, 322
Fnu4HI	GCNGC	7 cut(s)	28, 334, 460, 990, 1117, 2326, 2421
FriOI	GRGCYC	1 cut(s)	456
Fsp4HI	GCNGC	7 cut(s)	28, 334, 460, 990, 1117, 2326, 2421
FspBI	CTAG	3 cut(s)	105, 1113, 2417
FspI	TGCGCA	1 cut(s)	1778
GlaI	GCGC	5 cut(s)	310, 850, 1778, 2058, 2171
GluI	GCNGC	7 cut(s)	28, 334, 460, 990, 1117, 2326, 2421
GsaI	CCCAGC	2 cut(s)	855, 1758
HaeII	RGCGCY	1 cut(s)	852
HaeIII	GGCC	6 cut(s)	352, 459, 647, 1832, 2016, 2232
HhaI	GCGC	5 cut(s)	311, 851, 1779, 2059, 2172
Hin1II	CATG	8 cut(s)	89, 103, 937, 1270, 1379, 1521, 1717, 2353
Hin6I	GCGC	5 cut(s)	309, 849, 1777, 2057, 2170
HinP1I	GCGC	5 cut(s)	309, 849, 1777, 2057, 2170
HincII	GTYRAC	1 cut(s)	2143
HindII	GTYRAC	1 cut(s)	2143
HindIII	AAGCTT	1 cut(s)	1721
HinfI	GANTC	6 cut(s)	476, 1198, 1571, 2150, 2180, 2354
HphI	GGTGA	2 cut(s)	224, 1018
Hpy166II	GTNNAC	8 cut(s)	47, 215, 1162, 1453, 1555, 1601, 2103, 2143
Hpy188I	TCNGA	7 cut(s)	195, 254, 451, 783, 1051, 2260, 2397
Hpy188III	TCNNGA	6 cut(s)	326, 383, 431, 1424, 1473, 2164
Hpy8I	GTNNAC	8 cut(s)	47, 215, 1162, 1453, 1555, 1601, 2103, 2143
HpyCH4III	ACNGT	7 cut(s)	206, 212, 1894, 1947, 2075, 2302, 2368
HpyCH4IV	ACGT	3 cut(s)	49, 2079, 2099
HpyF3I	CTNAG	9 cut(s)	260, 371, 472, 557, 585, 683, 1048, 1308, 1684
HpySE526I	ACGT	3 cut(s)	49, 2079, 2099
Hsp92II	CATG	8 cut(s)	89, 103, 937, 1270, 1379, 1521, 1717, 2353
HspAI	GCGC	5 cut(s)	309, 849, 1777, 2057, 2170
Kpn2I	TCCGGA	2 cut(s)	430, 1472
KpnI	GGTACC	1 cut(s)	953
Ksp22I	TGATCA	2 cut(s)	18, 322
Kzo9I	GATC	8 cut(s)	18, 246, 322, 906, 962, 976, 1038, 1678
LguI	GCTCTTC	3 cut(s)	901, 1296, 2202
LmnI	GCTCC	3 cut(s)	85, 451, 1511
Lsp1109I	GCAGC	4 cut(s)	39, 1001, 1128, 2337
LweI	GCATC	7 cut(s)	320, 595, 1183, 1466, 2053, 2157, 2279
MaeI	CTAG	3 cut(s)	105, 1113, 2417
MaeII	ACGT	3 cut(s)	49, 2079, 2099
MaeIII	GTNAC	5 cut(s)	206, 1123, 1513, 1882, 2075
MalI	GATC	8 cut(s)	20, 248, 324, 908, 964, 978, 1040, 1680
MboI	GATC	8 cut(s)	18, 246, 322, 906, 962, 976, 1038, 1678
MfeI	CAATTG	2 cut(s)	631, 1501
MflI	RGATCY	3 cut(s)	962, 976, 1678
MhlI	GDGCHC	5 cut(s)	456, 733, 1291, 1530, 1603
MluCI	AATT	9 cut(s)	75, 232, 406, 552, 631, 1001, 1501, 2004, 2313
MlyI	GAGTC	2 cut(s)	485, 2363
MmeI	TCCRAC	3 cut(s)	429, 793, 1284
Mph1103I	ATGCAT	1 cut(s)	1481
MroI	TCCGGA	2 cut(s)	430, 1472
MroXI	GAANNNNTTC	1 cut(s)	1215
MseI	TTAA	1 cut(s)	918
MslI	CAYNNNNRTG	4 cut(s)	432, 1103, 1250, 1606
MspA1I	CMGCKG	4 cut(s)	30, 1348, 1701, 1977
MunI	CAATTG	2 cut(s)	631, 1501
Mva1269I	GAATGC	6 cut(s)	700, 765, 790, 844, 1646, 2306
MvaI	CCWGG	8 cut(s)	157, 654, 771, 1085, 1643, 1866, 2226, 2238
MvnI	CGCG	2 cut(s)	2057, 2172
NciI	CCSGG	5 cut(s)	243, 244, 1568, 1834, 1854
NdeI	CATATG	1 cut(s)	519
NdeII	GATC	8 cut(s)	18, 246, 322, 906, 962, 976, 1038, 1678
NlaIII	CATG	8 cut(s)	89, 103, 937, 1270, 1379, 1521, 1717, 2353
NlaIV	GGNNCC	6 cut(s)	112, 951, 1276, 1432, 1902, 2026
NmeAIII	GCCGAG	4 cut(s)	253, 378, 435, 447
NmuCI	GTSAC	2 cut(s)	206, 1882
NsbI	TGCGCA	1 cut(s)	1778
NsiI	ATGCAT	1 cut(s)	1481
NspI	RCATGY	3 cut(s)	89, 1379, 1717
PaeI	GCATGC	1 cut(s)	1717
PaeR7I	CTCGAG	1 cut(s)	1233
PciSI	GCTCTTC	3 cut(s)	901, 1296, 2202
PcsI	WCGNNNNNNNCGW	1 cut(s)	540
PctI	GAATGC	6 cut(s)	700, 765, 790, 844, 1646, 2306
PdmI	GAANNNNTTC	1 cut(s)	1215
PfeI	GAWTC	4 cut(s)	1198, 1571, 2150, 2180
PflMI	CCANNNNNTGG	1 cut(s)	2317
PfoI	TCCNGGA	1 cut(s)	1566
PinAI	ACCGGT	1 cut(s)	2137
PkrI	GCNGC	7 cut(s)	29, 335, 461, 991, 1118, 2327, 2422
PleI	GAGTC	2 cut(s)	484, 2362
PpsI	GAGTC	2 cut(s)	484, 2362
PpuMI	RGGWCCY	1 cut(s)	1430
Psp124BI	GAGCTC	1 cut(s)	456
Psp5II	RGGWCCY	1 cut(s)	1430
Psp6I	CCWGG	8 cut(s)	155, 652, 769, 1083, 1641, 1864, 2224, 2236
PspFI	CCCAGC	2 cut(s)	851, 1754
PspGI	CCWGG	8 cut(s)	155, 652, 769, 1083, 1641, 1864, 2224, 2236
PspN4I	GGNNCC	6 cut(s)	112, 951, 1276, 1432, 1902, 2026
PspPI	GGNCC	6 cut(s)	641, 1275, 1430, 1831, 2014, 2231
PspPPI	RGGWCCY	1 cut(s)	1430
PspXI	VCTCGAGB	1 cut(s)	1233
PsuI	RGATCY	3 cut(s)	962, 976, 1678
RsaI	GTAC	6 cut(s)	275, 368, 951, 971, 1082, 1485
RsaNI	GTAC	6 cut(s)	274, 367, 950, 970, 1081, 1484
RseI	CAYNNNNRTG	4 cut(s)	432, 1103, 1250, 1606
SacI	GAGCTC	1 cut(s)	456
SapI	GCTCTTC	3 cut(s)	901, 1296, 2202
SaqAI	TTAA	1 cut(s)	918
SatI	GCNGC	7 cut(s)	28, 334, 460, 990, 1117, 2326, 2421
Sau3AI	GATC	8 cut(s)	18, 246, 322, 906, 962, 976, 1038, 1678
Sau96I	GGNCC	6 cut(s)	641, 1275, 1430, 1831, 2014, 2231
SchI	GAGTC	2 cut(s)	485, 2363
SduI	GDGCHC	5 cut(s)	456, 733, 1291, 1530, 1603
SfaNI	GCATC	7 cut(s)	320, 595, 1183, 1466, 2053, 2157, 2279
SfcI	CTRYAG	2 cut(s)	2112, 2334
Sfr274I	CTCGAG	1 cut(s)	1233
SinI	GGWCC	3 cut(s)	641, 1275, 1430
SlaI	CTCGAG	1 cut(s)	1233
SmaI	CCCGGG	1 cut(s)	244
SmiMI	CAYNNNNRTG	4 cut(s)	432, 1103, 1250, 1606
SmlI	CTYRAG	1 cut(s)	1233
SmoI	CTYRAG	1 cut(s)	1233
SphI	GCATGC	1 cut(s)	1717
Sse9I	AATT	9 cut(s)	75, 232, 406, 552, 631, 1001, 1501, 2004, 2313
SspI	AATATT	1 cut(s)	1549
SspMI	CTAG	3 cut(s)	105, 1113, 2417
SstI	GAGCTC	1 cut(s)	456
StyI	CCWWGG	3 cut(s)	759, 1509, 2017
TaaI	ACNGT	7 cut(s)	206, 212, 1894, 1947, 2075, 2302, 2368
TaiI	ACGT	3 cut(s)	52, 2082, 2102
TaqI	TCGA	6 cut(s)	237, 479, 534, 700, 1201, 1234
TasI	AATT	9 cut(s)	75, 232, 406, 552, 631, 1001, 1501, 2004, 2313
TatI	WGTACW	2 cut(s)	969, 1483
TauI	GCSGC	2 cut(s)	336, 462
TfiI	GAWTC	4 cut(s)	1198, 1571, 2150, 2180
Tru1I	TTAA	1 cut(s)	918
Tru9I	TTAA	1 cut(s)	918
TscAI	CASTG	3 cut(s)	1226, 1537, 1581
TseFI	GTSAC	2 cut(s)	206, 1882
TseI	GCWGC	5 cut(s)	27, 989, 1116, 2325, 2420
Tsp45I	GTSAC	2 cut(s)	206, 1882
TspGWI	ACGGA	3 cut(s)	1331, 1349, 2259
TspMI	CCCGGG	1 cut(s)	242
TspRI	CASTG	3 cut(s)	1226, 1537, 1581
Van91I	CCANNNNNTGG	1 cut(s)	2317
VneI	GTGCAC	1 cut(s)	1599
VpaK11BI	GGWCC	3 cut(s)	641, 1275, 1430
XapI	RAATTY	3 cut(s)	232, 406, 2313
XceI	RCATGY	3 cut(s)	89, 1379, 1717
XcmI	CCANNNNNNNNNTGG	3 cut(s)	196, 757, 2347
XhoI	CTCGAG	1 cut(s)	1233
XmaI	CCCGGG	1 cut(s)	242
XmnI	GAANNNNTTC	1 cut(s)	1215
XspI	CTAG	3 cut(s)	105, 1113, 2417
Zsp2I	ATGCAT	1 cut(s)	1481

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.