Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0005618

Physical Location & Seq

Genomic Coordinates Forward (+)

33922972 .. 33925856

Gene Structure 2885 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1273200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1293 bp

ATGTCAATCCTATGTGCGTGTCCTGTCCTTGAGTGTGTTTATTGTCTGGCATGTACCCGCTGGGCGTGGAAGCGATGCCTCCACACAGCGGGTCATGACAGTGAGACTTGGGGCCTTTCCACTGCTGAAGAATTTGAGCCTGTTCCTCGCCTTTGCCGCTACATTCTAGCAGTTTACGAAGATGACCTTCGATGCCCTCTTTGGGAACCACCCGGTGGGTATGGAATTAACCCAGATTGGTTAATCTTGAAAAAGACTTATGAAGATACCGGAGGTCTAGCTCCTCCTTATATATTGTATCTTGATCACAATCATGCCGATATAGTTCTTGCCTTCAGAGGCCTTAACTTGGCAAGAGAGAGTGACTATGCAGTTCTGATGGATAATAAGCTCGGCCAGAGGAAATTTGATGGCGGGTATGTTCACAACGGGCTATTGAAATCTGCTCAATGGGTTATGGATGCAGAATGTGAAATTTTGAAAGATCTGGTGCAGAATTATCCAAATTATACTTTGACTTTCGCGGGACACTCCCTAGGGTCAGGTGTAGCAGCATTGTTAACAATGGTGGTAGTGAAGAACAGGGATAGGTTGGGGGACATTGATCGGAAGAGGGTTAGGGGCTATGCTATTGCACCGGCAAGGTGTATGTCATTGAACCTAGCTGTCAGATATGCAGACGTCATCAATTCTGTTGTGCTTCAGGATGACTTCTTACCGCGGACTGCTACACCCTTGGAAGACATTTTCAAGTCACTTTTCTGTTTGCCTTGCATATTGTGCCTACGATGCATGAGGGACACATGTATACCTGAGGAGAAGATGCTCAAAGATCCAAGGAGGCTCTATGCACCTGGCCGCCTTTATCACATTGTTGAGAGAAAGCCTTTTAGGTGTGGAAGATTTCCCCCCGTTGTCAAGACAGCAGTTCCCGTGGACGGGAGATTTGAGCACATCGTTCTTTCTTGTAATGCCACTTCTGACCACGCCATCATTTGGATAGAGAGAGAAGCCCAAAGGGCTCTGGATTTAATGTTGCAGAAAGATCATATTATGGAGATTCCGTCGAAGCAAAGAATGGAGCGGCAGGAGACATTAGCCAAAGAACACACAGAAGAGTACAAGGCTGCATTACAGAGGGCTGTCACATTGGCCGTGCCTCATGCATATTCACCGTCTCCGTACGGAACTTTTGACGAGAAGGACGAGGAAGATCACTCATATGGATCAAGCGGGGAATCTTCGTTTGGATCGACTAAGAAAAGCAAGTCCTTCACAGCGCGGTTTTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lipase3_N Lipase_3

Protein Analysis

431

Amino Acids

48.85

Weight (kDa)

6.51

Isoelectric Point (pI)

55.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase3_N	PF03893	10 - 75	7.6e-25	Lipase 3 N-terminal region
Lipase_3	PF01764	109 - 244	2.5e-26	Lipase (class 3)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005618)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49050
fragaria_vesca	FvH4_7g29770 FvH4_7g29770 FvH4_7g29770 FvH4_7g29770 FvH4_7g29770
malus_domestica	MD01G1204700.v1.1 MD07G1273200.v1.1
prunus_persica	Prupe.2G296900_v2.0.a1 Prupe.2G296900_v2.0.a1 Prupe.2G296900_v2.0.a1 Prupe.2G296900_v2.0.a1 Prupe.2G296900_v2.0.a1 Prupe.2G296900_v2.0.a1 Prupe.2G296900_v2.0.a1 Prupe.2G296900_v2.0.a1
pyrus_communis	pycom01g21420 pycom07g24970 pycom07g24980
rosa_chinensis	RchiOBHm_Chr1g0377761
rosa_multiflora	Rmu_co8163758.1_g000001 Rmu_sc0021958.1_g000001 Rmu_sc0024583.1_g000002
rosa_roxburghii	Rroxscaffold_4G00281080
rosa_rugosa	Rorug01G0404800
rosa_samantha	Rh1AG427500 Rh1CG395700
rosa_wichuraiana	Rw1G037100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	684
AccB7I	CCANNNNNTGG	2 cut(s)	215, 996
AccBSI	CCGCTC	1 cut(s)	1084
AccI	GTMKAC	1 cut(s)	808
AccII	CGCG	3 cut(s)	524, 721, 1282
AclWI	GGATC	3 cut(s)	827, 1234, 1258
AcoI	YGGCCR	3 cut(s)	394, 856, 1152
AcsI	RAATTY	3 cut(s)	131, 404, 474
AcuI	CTGAAG	3 cut(s)	147, 319, 686
AcyI	GRCGYC	1 cut(s)	681
AdeI	CACNNNGTG	1 cut(s)	215
AfaI	GTAC	3 cut(s)	55, 1121, 1184
AfiI	CCNNNNNNNGG	7 cut(s)	88, 202, 215, 349, 938, 939, 996
AflIII	ACRYGT	1 cut(s)	803
AgsI	TTSAA	5 cut(s)	250, 439, 481, 658, 751
AjnI	CCWGG	1 cut(s)	853
AjuI	GAANNNNNNNTTGG	2 cut(s)	1230, 1262
AluBI	AGCT	3 cut(s)	281, 391, 665
AluI	AGCT	3 cut(s)	281, 391, 665
Alw21I	GWGCWC	1 cut(s)	954
Alw26I	GTCTC	3 cut(s)	98, 1085, 1182
AlwI	GGATC	3 cut(s)	827, 1234, 1258
AoxI	GGCC	5 cut(s)	112, 340, 394, 856, 1152
ApeKI	GCWGC	2 cut(s)	551, 1127
ApoI	RAATTY	3 cut(s)	131, 404, 474
ArsI	GACNNNNNNTTYG	2 cut(s)	929, 961
AspA2I	CCTAGG	1 cut(s)	535
AspLEI	GCGC	1 cut(s)	1282
AspS9I	GGNCC	1 cut(s)	112
AsuC2I	CCSGG	1 cut(s)	213
AsuHPI	GGTGA	1 cut(s)	1164
AvrII	CCTAGG	1 cut(s)	535
AxyI	CCTNAGG	1 cut(s)	813
BanII	GRGCYC	1 cut(s)	1024
BarI	GAAGNNNNNNTAC	2 cut(s)	961, 993
BbsI	GAAGAC	1 cut(s)	747
Bbv12I	GWGCWC	1 cut(s)	954
BbvI	GCAGC	2 cut(s)	563, 1114
BccI	CCATC	3 cut(s)	373, 404, 998
BceAI	ACGGC	1 cut(s)	1139
BciT130I	CCWGG	1 cut(s)	855
BclI	TGATCA	1 cut(s)	304
BcnI	CCSGG	1 cut(s)	213
BcoDI	GTCTC	3 cut(s)	98, 1085, 1182
BfaI	CTAG	5 cut(s)	167, 278, 536, 662, 1291
BglI	GCCNNNNNGGC	1 cut(s)	1019
BglII	AGATCT	1 cut(s)	484
BisI	GCNGC	5 cut(s)	157, 552, 859, 1085, 1128
BlnI	CCTAGG	1 cut(s)	535
BlsI	GCNGC	5 cut(s)	158, 553, 860, 1086, 1129
Bme1390I	CCNGG	2 cut(s)	213, 855
BmgT120I	GGNCC	1 cut(s)	112
BmiI	GGNNCC	2 cut(s)	113, 207
BmrFI	CCNGG	2 cut(s)	213, 855
BmsI	GCATC	5 cut(s)	65, 182, 451, 779, 813
BpiI	GAAGAC	1 cut(s)	747
BpuEI	CTTGAG	1 cut(s)	50
BpuMI	CCSGG	1 cut(s)	213
BsaBI	GATNNNNATC	1 cut(s)	309
BsaHI	GRCGYC	1 cut(s)	681
BsaJI	CCNNGG	5 cut(s)	535, 719, 735, 836, 933
BsaWI	WCCGGW	1 cut(s)	269
BsaXI	ACNNNNNCTCC	2 cut(s)	1049, 1079
Bsc4I	CCNNNNNNNGG	7 cut(s)	88, 202, 215, 349, 938, 939, 996
Bse118I	RCCGGY	1 cut(s)	637
Bse21I	CCTNAGG	1 cut(s)	813
Bse8I	GATNNNNATC	1 cut(s)	309
BseBI	CCWGG	1 cut(s)	855
BseDI	CCNNGG	5 cut(s)	535, 719, 735, 836, 933
BseGI	GGATG	2 cut(s)	466, 712
BseJI	GATNNNNATC	1 cut(s)	309
BseLI	CCNNNNNNNGG	7 cut(s)	88, 202, 215, 349, 938, 939, 996
BseMII	CTCAG	1 cut(s)	804
BseRI	GAGGAG	2 cut(s)	273, 830
BseXI	GCAGC	2 cut(s)	563, 1114
BseYI	CCCAGC	1 cut(s)	60
BsgI	GTGCAG	1 cut(s)	512
Bsh1236I	CGCG	3 cut(s)	524, 721, 1282
BshFI	GGCC	5 cut(s)	114, 342, 396, 858, 1154
BsiHKAI	GWGCWC	1 cut(s)	954
BsiSI	CCGG	3 cut(s)	213, 270, 638
BsiWI	CGTACG	1 cut(s)	1182
BslFI	GGGAC	3 cut(s)	540, 611, 812
BslI	CCNNNNNNNGG	7 cut(s)	88, 202, 215, 349, 938, 939, 996
BsmAI	GTCTC	3 cut(s)	98, 1085, 1182
BsmBI	CGTCTC	1 cut(s)	1182
BsmFI	GGGAC	3 cut(s)	540, 611, 812
BsnI	GGCC	5 cut(s)	114, 342, 396, 858, 1154
Bsp1286I	GDGCHC	2 cut(s)	954, 1024
Bsp143I	GATC	8 cut(s)	304, 484, 604, 832, 1045, 1213, 1226, 1250
BspANI	GGCC	5 cut(s)	114, 342, 396, 858, 1154
BspCNI	CTCAG	1 cut(s)	805
BspFNI	CGCG	3 cut(s)	524, 721, 1282
BspHI	TCATGA	1 cut(s)	94
BspLI	GGNNCC	2 cut(s)	113, 207
BspPI	GGATC	3 cut(s)	827, 1234, 1258
BsrBI	CCGCTC	1 cut(s)	1084
BsrFI	RCCGGY	1 cut(s)	637
BssAI	RCCGGY	1 cut(s)	637
BssECI	CCNNGG	5 cut(s)	535, 719, 735, 836, 933
BssMI	GATC	8 cut(s)	304, 484, 604, 832, 1045, 1213, 1226, 1250
BssNAI	GTATAC	1 cut(s)	809
BssNI	GRCGYC	1 cut(s)	681
BssT1I	CCWWGG	3 cut(s)	535, 735, 836
Bst1107I	GTATAC	1 cut(s)	809
Bst2UI	CCWGG	1 cut(s)	855
Bst4CI	ACNGT	2 cut(s)	101, 1176
Bst6I	CTCTTC	2 cut(s)	605, 1110
BstACI	GRCGYC	1 cut(s)	681
BstAPI	GCANNNNNTGC	1 cut(s)	780
BstDEI	CTNAG	2 cut(s)	813, 1257
BstDSI	CCRYGG	2 cut(s)	719, 933
BstF5I	GGATG	2 cut(s)	466, 712
BstFNI	CGCG	3 cut(s)	524, 721, 1282
BstHHI	GCGC	1 cut(s)	1282
BstKTI	GATC	8 cut(s)	307, 487, 607, 835, 1048, 1216, 1229, 1253
BstMAI	GTCTC	3 cut(s)	98, 1085, 1182
BstMBI	GATC	8 cut(s)	304, 484, 604, 832, 1045, 1213, 1226, 1250
BstMWI	GCNNNNNNNGC	4 cut(s)	156, 780, 789, 1019
BstNI	CCWGG	1 cut(s)	855
BstNSI	RCATGY	2 cut(s)	54, 807
BstSCI	CCNGG	2 cut(s)	211, 853
BstUI	CGCG	3 cut(s)	524, 721, 1282
BstV1I	GCAGC	2 cut(s)	563, 1114
BstV2I	GAAGAC	1 cut(s)	747
BstX2I	RGATCY	2 cut(s)	484, 832
BstYI	RGATCY	2 cut(s)	484, 832
BstZ17I	GTATAC	1 cut(s)	809
Bsu36I	CCTNAGG	1 cut(s)	813
BsuRI	GGCC	5 cut(s)	114, 342, 396, 858, 1154
BtgI	CCRYGG	2 cut(s)	719, 933
BtgZI	GCGATG	1 cut(s)	88
BtsCI	GGATG	2 cut(s)	466, 712
BtsI	GCAGTG	1 cut(s)	120
BtsIMutI	CAGTG	2 cut(s)	106, 120
CciI	TCATGA	1 cut(s)	94
CfoI	GCGC	1 cut(s)	1282
Cfr10I	RCCGGY	1 cut(s)	637
Cfr13I	GGNCC	1 cut(s)	112
Cfr42I	CCGCGG	1 cut(s)	722
Csp6I	GTAC	3 cut(s)	54, 1120, 1183
CviAII	CATG	6 cut(s)	51, 95, 314, 793, 804, 1163
CviQI	GTAC	3 cut(s)	54, 1120, 1183
DdeI	CTNAG	2 cut(s)	813, 1257
DpnI	GATC	8 cut(s)	306, 486, 606, 834, 1047, 1215, 1228, 1252
DpnII	GATC	8 cut(s)	304, 484, 604, 832, 1045, 1213, 1226, 1250
DraIII	CACNNNGTG	1 cut(s)	215
EaeI	YGGCCR	3 cut(s)	394, 856, 1152
Eam1104I	CTCTTC	2 cut(s)	605, 1110
EarI	CTCTTC	2 cut(s)	605, 1110
Eco130I	CCWWGG	3 cut(s)	535, 735, 836
Eco147I	AGGCCT	1 cut(s)	342
Eco24I	GRGCYC	1 cut(s)	1024
Eco57I	CTGAAG	3 cut(s)	147, 319, 686
Eco81I	CCTNAGG	1 cut(s)	813
EcoO109I	RGGNCCY	1 cut(s)	112
EcoRII	CCWGG	1 cut(s)	853
EcoT14I	CCWWGG	3 cut(s)	535, 735, 836
EcoT22I	ATGCAT	2 cut(s)	794, 1168
EcoT38I	GRGCYC	1 cut(s)	1024
ErhI	CCWWGG	3 cut(s)	535, 735, 836
Esp3I	CGTCTC	1 cut(s)	1182
FaeI	CATG	6 cut(s)	54, 98, 317, 796, 807, 1166
FalI	AAGNNNNNCTT	2 cut(s)	171, 203
FaqI	GGGAC	3 cut(s)	540, 611, 812
FatI	CATG	6 cut(s)	50, 94, 313, 792, 803, 1162
FauI	CCCGC	5 cut(s)	65, 82, 407, 517, 1226
FauNDI	CATATG	1 cut(s)	1222
FbaI	TGATCA	1 cut(s)	304
FblI	GTMKAC	1 cut(s)	808
Fnu4HI	GCNGC	5 cut(s)	157, 552, 859, 1085, 1128
FokI	GGATG	2 cut(s)	473, 719
FriOI	GRGCYC	1 cut(s)	1024
Fsp4HI	GCNGC	5 cut(s)	157, 552, 859, 1085, 1128
FspBI	CTAG	5 cut(s)	167, 278, 536, 662, 1291
GlaI	GCGC	1 cut(s)	1281
GluI	GCNGC	5 cut(s)	157, 552, 859, 1085, 1128
GsaI	CCCAGC	1 cut(s)	64
HaeIII	GGCC	5 cut(s)	114, 342, 396, 858, 1154
HapII	CCGG	3 cut(s)	213, 270, 638
HhaI	GCGC	1 cut(s)	1282
Hin1I	GRCGYC	1 cut(s)	681
Hin1II	CATG	6 cut(s)	54, 98, 317, 796, 807, 1166
Hin6I	GCGC	1 cut(s)	1280
HinP1I	GCGC	1 cut(s)	1280
HincII	GTYRAC	1 cut(s)	561
HindII	GTYRAC	1 cut(s)	561
HinfI	GANTC	2 cut(s)	1060, 1238
HpaI	GTTAAC	1 cut(s)	561
HpaII	CCGG	3 cut(s)	213, 270, 638
HphI	GGTGA	1 cut(s)	1164
Hpy166II	GTNNAC	5 cut(s)	175, 424, 561, 809, 937
Hpy188I	TCNGA	5 cut(s)	338, 378, 609, 671, 982
Hpy188III	TCNNGA	6 cut(s)	95, 247, 302, 704, 919, 1025
Hpy8I	GTNNAC	5 cut(s)	175, 424, 561, 809, 937
Hpy99I	CGWCG	1 cut(s)	1069
HpyAV	CCTTC	4 cut(s)	197, 343, 1195, 1282
HpyCH4III	ACNGT	2 cut(s)	101, 1176
HpyCH4IV	ACGT	1 cut(s)	681
HpyF10VI	GCNNNNNNNGC	4 cut(s)	156, 780, 789, 1019
HpyF3I	CTNAG	2 cut(s)	813, 1257
HpySE526I	ACGT	1 cut(s)	681
Hsp92I	GRCGYC	1 cut(s)	681
Hsp92II	CATG	6 cut(s)	54, 98, 317, 796, 807, 1166
HspAI	GCGC	1 cut(s)	1280
Ksp22I	TGATCA	1 cut(s)	304
KspAI	GTTAAC	1 cut(s)	561
KspI	CCGCGG	1 cut(s)	722
Kzo9I	GATC	8 cut(s)	304, 484, 604, 832, 1045, 1213, 1226, 1250
LmnI	GCTCC	2 cut(s)	286, 1081
Lsp1109I	GCAGC	2 cut(s)	563, 1114
LweI	GCATC	5 cut(s)	65, 182, 451, 779, 813
MaeI	CTAG	5 cut(s)	167, 278, 536, 662, 1291
MaeII	ACGT	1 cut(s)	681
MaeIII	GTNAC	3 cut(s)	362, 753, 1144
MalI	GATC	8 cut(s)	306, 486, 606, 834, 1047, 1215, 1228, 1252
MbiI	CCGCTC	1 cut(s)	1084
MboI	GATC	8 cut(s)	304, 484, 604, 832, 1045, 1213, 1226, 1250
MflI	RGATCY	2 cut(s)	484, 832
MhlI	GDGCHC	2 cut(s)	954, 1024
MluCI	AATT	7 cut(s)	131, 225, 404, 474, 496, 505, 688
Mph1103I	ATGCAT	2 cut(s)	794, 1168
MseI	TTAA	5 cut(s)	228, 242, 345, 560, 1031
MslI	CAYNNNNRTG	3 cut(s)	99, 312, 1221
MspA1I	CMGCKG	3 cut(s)	60, 89, 721
MspI	CCGG	3 cut(s)	213, 270, 638
MspR9I	CCNGG	2 cut(s)	213, 855
MvaI	CCWGG	1 cut(s)	855
MvnI	CGCG	3 cut(s)	524, 721, 1282
MwoI	GCNNNNNNNGC	4 cut(s)	156, 780, 789, 1019
NciI	CCSGG	1 cut(s)	213
NdeI	CATATG	1 cut(s)	1222
NdeII	GATC	8 cut(s)	304, 484, 604, 832, 1045, 1213, 1226, 1250
NlaIII	CATG	6 cut(s)	54, 98, 317, 796, 807, 1166
NlaIV	GGNNCC	2 cut(s)	113, 207
NmeAIII	GCCGAG	1 cut(s)	372
NmuCI	GTSAC	3 cut(s)	362, 753, 1144
NsiI	ATGCAT	2 cut(s)	794, 1168
NspI	RCATGY	2 cut(s)	54, 807
PagI	TCATGA	1 cut(s)	94
PceI	AGGCCT	1 cut(s)	342
PciI	ACATGT	1 cut(s)	803
PcsI	WCGNNNNNNNCGW	2 cut(s)	1203, 1250
PfeI	GAWTC	2 cut(s)	1060, 1238
Pfl23II	CGTACG	1 cut(s)	1182
PflMI	CCANNNNNTGG	2 cut(s)	215, 996
PkrI	GCNGC	5 cut(s)	158, 553, 860, 1086, 1129
PscI	ACATGT	1 cut(s)	803
Psp6I	CCWGG	1 cut(s)	853
PspFI	CCCAGC	1 cut(s)	60
PspGI	CCWGG	1 cut(s)	853
PspLI	CGTACG	1 cut(s)	1182
PspN4I	GGNNCC	2 cut(s)	113, 207
PspPI	GGNCC	1 cut(s)	112
PsuI	RGATCY	2 cut(s)	484, 832
RsaI	GTAC	3 cut(s)	55, 1121, 1184
RsaNI	GTAC	3 cut(s)	54, 1120, 1183
RseI	CAYNNNNRTG	3 cut(s)	99, 312, 1221
SacII	CCGCGG	1 cut(s)	722
SaqAI	TTAA	5 cut(s)	228, 242, 345, 560, 1031
SatI	GCNGC	5 cut(s)	157, 552, 859, 1085, 1128
Sau3AI	GATC	8 cut(s)	304, 484, 604, 832, 1045, 1213, 1226, 1250
Sau96I	GGNCC	1 cut(s)	112
ScrFI	CCNGG	2 cut(s)	213, 855
SduI	GDGCHC	2 cut(s)	954, 1024
SfaNI	GCATC	5 cut(s)	65, 182, 451, 779, 813
Sfr303I	CCGCGG	1 cut(s)	722
SgrBI	CCGCGG	1 cut(s)	722
SmiMI	CAYNNNNRTG	3 cut(s)	99, 312, 1221
SmlI	CTYRAG	1 cut(s)	29
SmoI	CTYRAG	1 cut(s)	29
Sse9I	AATT	7 cut(s)	131, 225, 404, 474, 496, 505, 688
SseBI	AGGCCT	1 cut(s)	342
SspMI	CTAG	5 cut(s)	167, 278, 536, 662, 1291
StuI	AGGCCT	1 cut(s)	342
StyD4I	CCNGG	2 cut(s)	211, 853
StyI	CCWWGG	3 cut(s)	535, 735, 836
TaaI	ACNGT	2 cut(s)	101, 1176
TaiI	ACGT	1 cut(s)	684
TaqI	TCGA	3 cut(s)	190, 1067, 1253
TasI	AATT	7 cut(s)	131, 225, 404, 474, 496, 505, 688
TatI	WGTACW	1 cut(s)	1119
TauI	GCSGC	3 cut(s)	159, 861, 1087
TfiI	GAWTC	2 cut(s)	1060, 1238
Tru1I	TTAA	5 cut(s)	228, 242, 345, 560, 1031
Tru9I	TTAA	5 cut(s)	228, 242, 345, 560, 1031
TscAI	CASTG	2 cut(s)	106, 127
TseFI	GTSAC	3 cut(s)	362, 753, 1144
TseI	GCWGC	2 cut(s)	551, 1127
Tsp45I	GTSAC	3 cut(s)	362, 753, 1144
TspDTI	ATGAA	1 cut(s)	276
TspGWI	ACGGA	3 cut(s)	1053, 1170, 1200
TspRI	CASTG	2 cut(s)	106, 127
Van91I	CCANNNNNTGG	2 cut(s)	215, 996
XapI	RAATTY	3 cut(s)	131, 404, 474
XceI	RCATGY	2 cut(s)	54, 807
XmaJI	CCTAGG	1 cut(s)	535
XmiI	GTMKAC	1 cut(s)	808
XspI	CTAG	5 cut(s)	167, 278, 536, 662, 1291
ZraI	GACGTC	1 cut(s)	682
Zsp2I	ATGCAT	2 cut(s)	794, 1168

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.