pycom07g24970

Lipase 3 N-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005618
Physical Location & Seq
Forward (+)
25457435 .. 25457761
327 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g24970.1

Sequence Viewer

Length: 327 bp
ATGTCAATCCTATGTGGGTGTCCTATCCTTGAGTGTGTTTATTGTCTGGCATGTACCCGTTGGGCGTGGAAGCGATGCCTCCACACAGCGGGTCATGACAGTGAGACTTGGGGCCTTTCCACTGCTGAAGAATTCGAGCCTGTTCCTCGCCTTTGCCGCTACATTCTAGCAGTTTACGAAGATGACCTTCGATGCCCTCTTTGGGAACCGCCCGGTGGGTATGGAATTAACCCAGATTGGTTAATCTTGAAAAAGACTTATGAAGATACCGGAGGTCTAGCTCCTCCTTATATATTGTATCTTGATCGATCTTTTTCTTCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase3_N Lipase_3

Protein Analysis

109

Amino Acids

12.35

Weight (kDa)

5.04

Isoelectric Point (pI)

69.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase3_N PF03893 10 - 75 5.1e-26 Lipase 3 N-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 89, 157, 209
AcsI RAATTY 1 cut(s) 131
AcuI CTGAAG 1 cut(s) 147
AfaI GTAC 1 cut(s) 55
AfiI CCNNNNNNNGG 3 cut(s) 88, 202, 215
AgsI TTSAA 1 cut(s) 250
AluBI AGCT 1 cut(s) 281
AluI AGCT 1 cut(s) 281
Alw26I GTCTC 1 cut(s) 98
AoxI GGCC 1 cut(s) 112
ApoI RAATTY 1 cut(s) 131
AspS9I GGNCC 1 cut(s) 112
AsuC2I CCSGG 1 cut(s) 213
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 98
BfaI CTAG 2 cut(s) 167, 278
BisI GCNGC 1 cut(s) 157
BlsI GCNGC 1 cut(s) 158
Bme1390I CCNGG 1 cut(s) 213
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 2 cut(s) 113, 207
BmrFI CCNGG 1 cut(s) 213
BmsI GCATC 2 cut(s) 65, 182
BpuEI CTTGAG 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 213
Bsa29I ATCGAT 1 cut(s) 307
BsaWI WCCGGW 1 cut(s) 269
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 202, 215
BseCI ATCGAT 1 cut(s) 307
BseLI CCNNNNNNNGG 3 cut(s) 88, 202, 215
BseRI GAGGAG 1 cut(s) 273
BshFI GGCC 1 cut(s) 114
BshVI ATCGAT 1 cut(s) 307
BsiSI CCGG 2 cut(s) 213, 270
BslI CCNNNNNNNGG 3 cut(s) 88, 202, 215
BsmAI GTCTC 1 cut(s) 98
BsnI GGCC 1 cut(s) 114
Bsp143I GATC 2 cut(s) 304, 308
BspACI CCGC 3 cut(s) 89, 157, 209
BspANI GGCC 1 cut(s) 114
BspDI ATCGAT 1 cut(s) 307
BspHI TCATGA 1 cut(s) 94
BspLI GGNNCC 2 cut(s) 113, 207
BssMI GATC 2 cut(s) 304, 308
Bst4CI ACNGT 1 cut(s) 101
BstKTI GATC 2 cut(s) 307, 311
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 2 cut(s) 304, 308
BstMWI GCNNNNNNNGC 1 cut(s) 156
BstNSI RCATGY 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 211
Bsu15I ATCGAT 1 cut(s) 307
BsuRI GGCC 1 cut(s) 114
BsuTUI ATCGAT 1 cut(s) 307
BtgZI GCGATG 1 cut(s) 88
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 2 cut(s) 106, 120
CciI TCATGA 1 cut(s) 94
Cfr13I GGNCC 1 cut(s) 112
ClaI ATCGAT 1 cut(s) 307
Csp6I GTAC 1 cut(s) 54
CviAII CATG 2 cut(s) 51, 95
CviJI RGCY 3 cut(s) 114, 139, 281
CviKI_1 RGCY 3 cut(s) 114, 139, 281
CviQI GTAC 1 cut(s) 54
DpnI GATC 2 cut(s) 306, 310
DpnII GATC 2 cut(s) 304, 308
Eco57I CTGAAG 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 112
EcoRI GAATTC 1 cut(s) 131
FaeI CATG 2 cut(s) 54, 98
FaiI YATR 7 cut(s) 13, 52, 96, 222, 261, 291, 293
FalI AAGNNNNNCTT 2 cut(s) 171, 203
FatI CATG 2 cut(s) 50, 94
FauI CCCGC 1 cut(s) 82
Fnu4HI GCNGC 1 cut(s) 157
Fsp4HI GCNGC 1 cut(s) 157
FspBI CTAG 2 cut(s) 167, 278
GluI GCNGC 1 cut(s) 157
HaeIII GGCC 1 cut(s) 114
HapII CCGG 2 cut(s) 213, 270
Hin1II CATG 2 cut(s) 54, 98
HpaII CCGG 2 cut(s) 213, 270
Hpy166II GTNNAC 1 cut(s) 175
Hpy188III TCNNGA 3 cut(s) 95, 247, 302
Hpy8I GTNNAC 1 cut(s) 175
HpyAV CCTTC 1 cut(s) 197
HpyCH4III ACNGT 1 cut(s) 101
HpyF10VI GCNNNNNNNGC 1 cut(s) 156
Hsp92II CATG 2 cut(s) 54, 98
Kzo9I GATC 2 cut(s) 304, 308
LmnI GCTCC 1 cut(s) 286
LpnPI CCDG 5 cut(s) 32, 153, 226, 246, 283
LweI GCATC 2 cut(s) 65, 182
MaeI CTAG 2 cut(s) 167, 278
MalI GATC 2 cut(s) 306, 310
MboI GATC 2 cut(s) 304, 308
MboII GAAGA 4 cut(s) 140, 191, 275, 309
MluCI AATT 2 cut(s) 131, 225
MnlI CCTC 5 cut(s) 89, 156, 207, 266, 294
MseI TTAA 2 cut(s) 228, 242
MslI CAYNNNNRTG 1 cut(s) 99
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 2 cut(s) 213, 270
MspR9I CCNGG 1 cut(s) 213
MwoI GCNNNNNNNGC 1 cut(s) 156
NciI CCSGG 1 cut(s) 213
NdeII GATC 2 cut(s) 304, 308
NlaIII CATG 2 cut(s) 54, 98
NlaIV GGNNCC 2 cut(s) 113, 207
NspI RCATGY 1 cut(s) 54
PagI TCATGA 1 cut(s) 94
PkrI GCNGC 1 cut(s) 158
PspN4I GGNNCC 2 cut(s) 113, 207
PspPI GGNCC 1 cut(s) 112
RsaI GTAC 1 cut(s) 55
RsaNI GTAC 1 cut(s) 54
RseI CAYNNNNRTG 1 cut(s) 99
SaqAI TTAA 2 cut(s) 228, 242
SatI GCNGC 1 cut(s) 157
Sau3AI GATC 2 cut(s) 304, 308
Sau96I GGNCC 1 cut(s) 112
ScrFI CCNGG 1 cut(s) 213
SetI ASST 3 cut(s) 189, 277, 283
SfaNI GCATC 2 cut(s) 65, 182
SmiMI CAYNNNNRTG 1 cut(s) 99
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 2 cut(s) 131, 225
SsiI CCGC 3 cut(s) 89, 157, 209
SspMI CTAG 2 cut(s) 167, 278
StyD4I CCNGG 1 cut(s) 211
TaaI ACNGT 1 cut(s) 101
TaqI TCGA 3 cut(s) 135, 190, 307
TasI AATT 2 cut(s) 131, 225
TauI GCSGC 1 cut(s) 159
Tru1I TTAA 2 cut(s) 228, 242
Tru9I TTAA 2 cut(s) 228, 242
TscAI CASTG 2 cut(s) 106, 127
TspDTI ATGAA 1 cut(s) 276
TspRI CASTG 2 cut(s) 106, 127
XapI RAATTY 1 cut(s) 131
XceI RCATGY 1 cut(s) 54
XspI CTAG 2 cut(s) 167, 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.