pycom01g21420

Lipase 3 N-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0005618
Physical Location & Seq
Forward (+)
19466239 .. 19468026
1788 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21420.1

Sequence Viewer

Length: 927 bp
ATGGATAATAAGCTGGGTAAGAGGAAATTTGATGGCGGGTATGTTCACAACGGGCTATTGAAGGCTGCTCAATGGGTCATGGATGCAGAATGTGAAATGTTGAAAGATCTGGTGGAGAAGTATCCAAAATATACTTTGACTTTCACAGGACACTCCCTAGGGTCAGGTGTAGCTGCATTGTTAACAATGTTGGTAGTGCAGAACAGGGATAGGTTGGGGGACATTGATCGGAAGAGGGTTAGGGGCTATGCTATTGCACCGGCGAGGTGTATGTCATTGAACTTAGCAGTCAGATATGCAGATGTCATCAATTCTGTTGTGCTTCAGGATGACTTTTTACCGCGGACTGCTACACCCTTGGAAGACATTTTCAAGTCACTTTTCTGTTTGCCGTGCATATTGTGCCTACGATGCATGAGGGACACATGTATACCTGAGGAGAAAATGCTCAAAGATCCAAGGAGGCTCTATGCACCTGGCCGCCTCTATCACATTGTTGAGAGAAAGCCTTTTAGGTGTGGAAGATTTCCTCCCGTTGTCAAGACAGCGGTTCCGGTGGATGGGAGATTTGAGCACATCGTTCTTTCTTGTAATGCCACTTCTGACCACGCTATCATTTGGATAGAGAGAGAAGCCCAAAGGGCTCTAGATTTATTATTGGAGAAAGATCAAATCATGGAGATTCCATCGAAGCAAAAAATGGAGCGGCAGGAGACGATAGCCAAAGAGCACTCGGAAGAGTACAAGGCTGCATTACAGAGGGCTGTCACATTGGCCGTGCCTCATGCATATTCACCGTCTCCGTATGGAACTTTTGACGAGAAGGACGAGGAAGATCACTCGTATGGATCGAGCGGGGAGTCTTCGGTTGGTTCGACGAAGAAAAGCAAGTCATTCACGGCGCGGTTTTTCGAGAGAAAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase3_N Lipase_3

Protein Analysis

309

Amino Acids

35.04

Weight (kDa)

8.47

Isoelectric Point (pI)

49.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 6 - 118 5.3e-25 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 706, 855
AccI GTMKAC 1 cut(s) 430
AccII CGCG 2 cut(s) 343, 904
AciI CCGC 8 cut(s) 36, 341, 343, 481, 548, 706, 855, 904
AclWI GGATC 2 cut(s) 449, 856
AcoI YGGCCR 2 cut(s) 478, 774
AcsI RAATTY 1 cut(s) 26
AcuI CTGAAG 1 cut(s) 308
AfaI GTAC 1 cut(s) 743
AfiI CCNNNNNNNGG 1 cut(s) 560
AflIII ACRYGT 1 cut(s) 425
AgsI TTSAA 4 cut(s) 61, 103, 280, 373
AjnI CCWGG 1 cut(s) 475
AluBI AGCT 2 cut(s) 13, 173
AluI AGCT 2 cut(s) 13, 173
Alw21I GWGCWC 2 cut(s) 576, 732
Alw26I GTCTC 2 cut(s) 707, 804
AlwI GGATC 2 cut(s) 449, 856
AoxI GGCC 2 cut(s) 478, 774
ApeKI GCWGC 3 cut(s) 65, 173, 749
ApoI RAATTY 1 cut(s) 26
AspA2I CCTAGG 1 cut(s) 157
AspLEI GCGC 1 cut(s) 904
AsuHPI GGTGA 1 cut(s) 786
AvrII CCTAGG 1 cut(s) 157
AxyI CCTNAGG 1 cut(s) 435
BanII GRGCYC 1 cut(s) 646
BarI GAAGNNNNNNTAC 2 cut(s) 583, 615
BbsI GAAGAC 2 cut(s) 369, 855
Bbv12I GWGCWC 2 cut(s) 576, 732
BbvI GCAGC 3 cut(s) 52, 160, 736
BccI CCATC 3 cut(s) 26, 554, 694
BceAI ACGGC 3 cut(s) 376, 761, 915
BciT130I CCWGG 1 cut(s) 477
BciVI GTATCC 1 cut(s) 132
BcoDI GTCTC 2 cut(s) 707, 804
BfaI CTAG 2 cut(s) 158, 647
BfuI GTATCC 1 cut(s) 132
BglI GCCNNNNNGGC 1 cut(s) 641
BglII AGATCT 1 cut(s) 106
BisI GCNGC 5 cut(s) 66, 174, 481, 707, 750
BlnI CCTAGG 1 cut(s) 157
BlsI GCNGC 5 cut(s) 67, 175, 482, 708, 751
Bme1390I CCNGG 1 cut(s) 477
BmiI GGNNCC 1 cut(s) 552
BmrFI CCNGG 1 cut(s) 477
BmsI GCATC 2 cut(s) 73, 401
BpiI GAAGAC 2 cut(s) 369, 855
BsaJI CCNNGG 4 cut(s) 157, 341, 357, 458
BsaWI WCCGGW 1 cut(s) 553
Bsc4I CCNNNNNNNGG 1 cut(s) 560
Bse118I RCCGGY 1 cut(s) 259
Bse21I CCTNAGG 1 cut(s) 435
BseBI CCWGG 1 cut(s) 477
BseDI CCNNGG 4 cut(s) 157, 341, 357, 458
BseGI GGATG 3 cut(s) 88, 334, 565
BseLI CCNNNNNNNGG 1 cut(s) 560
BseMII CTCAG 1 cut(s) 426
BseRI GAGGAG 1 cut(s) 452
BseXI GCAGC 3 cut(s) 52, 160, 736
BseYI CCCAGC 1 cut(s) 13
BsgI GTGCAG 1 cut(s) 218
Bsh1236I CGCG 2 cut(s) 343, 904
BshFI GGCC 2 cut(s) 480, 776
BsiHKAI GWGCWC 2 cut(s) 576, 732
BsiSI CCGG 2 cut(s) 260, 554
BslFI GGGAC 2 cut(s) 233, 434
BslI CCNNNNNNNGG 1 cut(s) 560
BsmAI GTCTC 2 cut(s) 707, 804
BsmBI CGTCTC 2 cut(s) 707, 804
BsmFI GGGAC 2 cut(s) 233, 434
BsnI GGCC 2 cut(s) 480, 776
Bsp1286I GDGCHC 3 cut(s) 576, 646, 732
Bsp143I GATC 6 cut(s) 106, 226, 454, 667, 835, 848
BspACI CCGC 8 cut(s) 36, 341, 343, 481, 548, 706, 855, 904
BspANI GGCC 2 cut(s) 480, 776
BspCNI CTCAG 1 cut(s) 427
BspFNI CGCG 2 cut(s) 343, 904
BspLI GGNNCC 1 cut(s) 552
BspPI GGATC 2 cut(s) 449, 856
BsrBI CCGCTC 2 cut(s) 706, 855
BsrFI RCCGGY 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 259
BssECI CCNNGG 4 cut(s) 157, 341, 357, 458
BssMI GATC 6 cut(s) 106, 226, 454, 667, 835, 848
BssNAI GTATAC 1 cut(s) 431
BssT1I CCWWGG 3 cut(s) 157, 357, 458
Bst1107I GTATAC 1 cut(s) 431
Bst2UI CCWGG 1 cut(s) 477
Bst4CI ACNGT 1 cut(s) 798
Bst6I CTCTTC 2 cut(s) 227, 732
BstAPI GCANNNNNTGC 1 cut(s) 402
BstDEI CTNAG 2 cut(s) 283, 435
BstDSI CCRYGG 1 cut(s) 341
BstF5I GGATG 3 cut(s) 88, 334, 565
BstFNI CGCG 2 cut(s) 343, 904
BstHHI GCGC 1 cut(s) 904
BstKTI GATC 6 cut(s) 109, 229, 457, 670, 838, 851
BstMAI GTCTC 2 cut(s) 707, 804
BstMBI GATC 6 cut(s) 106, 226, 454, 667, 835, 848
BstMWI GCNNNNNNNGC 3 cut(s) 402, 411, 641
BstNI CCWGG 1 cut(s) 477
BstNSI RCATGY 1 cut(s) 429
BstSCI CCNGG 1 cut(s) 475
BstUI CGCG 2 cut(s) 343, 904
BstV1I GCAGC 3 cut(s) 52, 160, 736
BstV2I GAAGAC 2 cut(s) 369, 855
BstX2I RGATCY 2 cut(s) 106, 454
BstYI RGATCY 2 cut(s) 106, 454
BstZ17I GTATAC 1 cut(s) 431
Bsu36I CCTNAGG 1 cut(s) 435
BsuI GTATCC 1 cut(s) 132
BsuRI GGCC 2 cut(s) 480, 776
BtgI CCRYGG 1 cut(s) 341
BtsCI GGATG 3 cut(s) 88, 334, 565
CfoI GCGC 1 cut(s) 904
Cfr10I RCCGGY 1 cut(s) 259
Cfr42I CCGCGG 1 cut(s) 344
Csp6I GTAC 1 cut(s) 742
CviAII CATG 5 cut(s) 79, 415, 426, 676, 785
CviQI GTAC 1 cut(s) 742
DdeI CTNAG 2 cut(s) 283, 435
DpnI GATC 6 cut(s) 108, 228, 456, 669, 837, 850
DpnII GATC 6 cut(s) 106, 226, 454, 667, 835, 848
EaeI YGGCCR 2 cut(s) 478, 774
Eam1104I CTCTTC 2 cut(s) 227, 732
EarI CTCTTC 2 cut(s) 227, 732
Eco130I CCWWGG 3 cut(s) 157, 357, 458
Eco24I GRGCYC 1 cut(s) 646
Eco57I CTGAAG 1 cut(s) 308
Eco81I CCTNAGG 1 cut(s) 435
EcoRII CCWGG 1 cut(s) 475
EcoT14I CCWWGG 3 cut(s) 157, 357, 458
EcoT22I ATGCAT 2 cut(s) 416, 790
EcoT38I GRGCYC 1 cut(s) 646
ErhI CCWWGG 3 cut(s) 157, 357, 458
Esp3I CGTCTC 2 cut(s) 707, 804
FaeI CATG 5 cut(s) 82, 418, 429, 679, 788
FaqI GGGAC 2 cut(s) 233, 434
FatI CATG 5 cut(s) 78, 414, 425, 675, 784
FauI CCCGC 2 cut(s) 29, 848
FblI GTMKAC 1 cut(s) 430
Fnu4HI GCNGC 5 cut(s) 66, 174, 481, 707, 750
FokI GGATG 3 cut(s) 95, 341, 572
FriOI GRGCYC 1 cut(s) 646
Fsp4HI GCNGC 5 cut(s) 66, 174, 481, 707, 750
FspBI CTAG 2 cut(s) 158, 647
GlaI GCGC 1 cut(s) 903
GluI GCNGC 5 cut(s) 66, 174, 481, 707, 750
GsaI CCCAGC 1 cut(s) 17
HaeIII GGCC 2 cut(s) 480, 776
HapII CCGG 2 cut(s) 260, 554
HhaI GCGC 1 cut(s) 904
Hin1II CATG 5 cut(s) 82, 418, 429, 679, 788
Hin6I GCGC 1 cut(s) 902
HinP1I GCGC 1 cut(s) 902
HincII GTYRAC 1 cut(s) 183
HindII GTYRAC 1 cut(s) 183
HinfI GANTC 2 cut(s) 682, 860
HpaI GTTAAC 1 cut(s) 183
HpaII CCGG 2 cut(s) 260, 554
HphI GGTGA 1 cut(s) 786
Hpy166II GTNNAC 3 cut(s) 46, 183, 431
Hpy188I TCNGA 4 cut(s) 231, 293, 604, 736
Hpy188III TCNNGA 4 cut(s) 326, 541, 647, 913
Hpy8I GTNNAC 3 cut(s) 46, 183, 431
Hpy99I CGWCG 1 cut(s) 880
HpyAV CCTTC 2 cut(s) 55, 817
HpyCH4III ACNGT 1 cut(s) 798
HpyF10VI GCNNNNNNNGC 3 cut(s) 402, 411, 641
HpyF3I CTNAG 2 cut(s) 283, 435
Hsp92II CATG 5 cut(s) 82, 418, 429, 679, 788
HspAI GCGC 1 cut(s) 902
KspAI GTTAAC 1 cut(s) 183
KspI CCGCGG 1 cut(s) 344
Kzo9I GATC 6 cut(s) 106, 226, 454, 667, 835, 848
LmnI GCTCC 1 cut(s) 703
Lsp1109I GCAGC 3 cut(s) 52, 160, 736
LweI GCATC 2 cut(s) 73, 401
MaeI CTAG 2 cut(s) 158, 647
MaeIII GTNAC 2 cut(s) 375, 766
MalI GATC 6 cut(s) 108, 228, 456, 669, 837, 850
MbiI CCGCTC 2 cut(s) 706, 855
MboI GATC 6 cut(s) 106, 226, 454, 667, 835, 848
MboII GAAGA 7 cut(s) 244, 374, 534, 749, 845, 855, 892
MflI RGATCY 2 cut(s) 106, 454
MhlI GDGCHC 3 cut(s) 576, 646, 732
MluCI AATT 2 cut(s) 26, 310
MlyI GAGTC 1 cut(s) 869
Mph1103I ATGCAT 2 cut(s) 416, 790
MseI TTAA 1 cut(s) 182
MslI CAYNNNNRTG 1 cut(s) 843
MspA1I CMGCKG 2 cut(s) 343, 548
MspI CCGG 2 cut(s) 260, 554
MspR9I CCNGG 1 cut(s) 477
MvaI CCWGG 1 cut(s) 477
MvnI CGCG 2 cut(s) 343, 904
MwoI GCNNNNNNNGC 3 cut(s) 402, 411, 641
NdeII GATC 6 cut(s) 106, 226, 454, 667, 835, 848
NlaIII CATG 5 cut(s) 82, 418, 429, 679, 788
NlaIV GGNNCC 1 cut(s) 552
NmuCI GTSAC 2 cut(s) 375, 766
NsiI ATGCAT 2 cut(s) 416, 790
NspI RCATGY 1 cut(s) 429
PciI ACATGT 1 cut(s) 425
PcsI WCGNNNNNNNCGW 3 cut(s) 825, 848, 872
PfeI GAWTC 1 cut(s) 682
PkrI GCNGC 5 cut(s) 67, 175, 482, 708, 751
PleI GAGTC 1 cut(s) 868
PpsI GAGTC 1 cut(s) 868
PscI ACATGT 1 cut(s) 425
Psp6I CCWGG 1 cut(s) 475
PspFI CCCAGC 1 cut(s) 13
PspGI CCWGG 1 cut(s) 475
PspN4I GGNNCC 1 cut(s) 552
PsuI RGATCY 2 cut(s) 106, 454
RsaI GTAC 1 cut(s) 743
RsaNI GTAC 1 cut(s) 742
RseI CAYNNNNRTG 1 cut(s) 843
SacII CCGCGG 1 cut(s) 344
SaqAI TTAA 1 cut(s) 182
SatI GCNGC 5 cut(s) 66, 174, 481, 707, 750
Sau3AI GATC 6 cut(s) 106, 226, 454, 667, 835, 848
SchI GAGTC 1 cut(s) 869
ScrFI CCNGG 1 cut(s) 477
SduI GDGCHC 3 cut(s) 576, 646, 732
SetI ASST 8 cut(s) 15, 169, 175, 215, 269, 436, 478, 518
SfaNI GCATC 2 cut(s) 73, 401
Sfr303I CCGCGG 1 cut(s) 344
SgrAI CRCCGGYG 1 cut(s) 259
SgrBI CCGCGG 1 cut(s) 344
SmiMI CAYNNNNRTG 1 cut(s) 843
Sse9I AATT 2 cut(s) 26, 310
SsiI CCGC 8 cut(s) 36, 341, 343, 481, 548, 706, 855, 904
SspMI CTAG 2 cut(s) 158, 647
StyD4I CCNGG 1 cut(s) 475
StyI CCWWGG 3 cut(s) 157, 357, 458
TaaI ACNGT 1 cut(s) 798
TaqI TCGA 4 cut(s) 689, 851, 875, 912
TasI AATT 2 cut(s) 26, 310
TatI WGTACW 1 cut(s) 741
TauI GCSGC 2 cut(s) 483, 709
TfiI GAWTC 1 cut(s) 682
Tru1I TTAA 1 cut(s) 182
Tru9I TTAA 1 cut(s) 182
TseFI GTSAC 2 cut(s) 375, 766
TseI GCWGC 3 cut(s) 65, 173, 749
Tsp45I GTSAC 2 cut(s) 375, 766
TspGWI ACGGA 1 cut(s) 792
XapI RAATTY 1 cut(s) 26
XbaI TCTAGA 1 cut(s) 646
XceI RCATGY 1 cut(s) 429
XmaJI CCTAGG 1 cut(s) 157
XmiI GTMKAC 1 cut(s) 430
XspI CTAG 2 cut(s) 158, 647
Zsp2I ATGCAT 2 cut(s) 416, 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.