MD07G1278400.v1.1

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013336
Physical Location & Seq
Forward (+)
34323189 .. 34326337
3149 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1278400.v1.1.491

Sequence Viewer

Length: 750 bp
ATGGCGGAGGTGGTGCTGCATATATACGATGTGACGAATAGTGGATCGGACAAGACGAATAGTACCATTCTGCAGATCAACAAGATCTTCAAAGACGGTATCGGCCTCGGCGGCATCTTCCACAGCGCCGTTCAGGTTTATGGAGAGGATGAATGGTCATTTGGGTTCTGTGAACAAGGATCTGGTGTGTTTAGTTGCCCTTCTGGAAAAAATCCAATGTACACATATCGTGAAAGCATCGTCCTTGGGACAACAGATTGTTCGATCTTCAAGGTTAACCAAATCTTGAGGGAACTCAGTAGAGAGTGGCCTGGGTATTCGTATGACCTGTTATCAAAAAACTGCAATCACTTTTGTGATGAGCTTTGTGAAAGGCTTGGGGTGCCAAAGCTTCCAGGTTGGGTTAATCGTTTTGCGCATGCTGGTGATGCTGCTATGGAAGTTGCAGGAAACACTGCAGTACGGTTTAGACAAGCGAAGACAGAGATTGTTACAGCTAGCAAAGTAGCATATCGTTTCCTTGTGGGTGTTACTAATAACGCCACAGCTTCTCCCGAGTCTGCAGGAAATTCAAACAGAGGTGCTCCTAGATTTCAAGCAGCTTGGTTCAAAAACCTAATCACCACAGGAGCAAAGCCATCTAGTAGTTCAGATCTCGAGACCAAGGAAGAAGATGCACTCCGGCAGCACCAGCAACCAGATGCAGAGCCGCAGCTGCAGCAGAATTCTCGGACATGGCAAAGTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

250

Amino Acids

27.5

Weight (kDa)

5.98

Isoelectric Point (pI)

30.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 3 - 147 7e-43 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013336)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25660 AT4G25680
fragaria_vesca FvH4_7g30180
malus_domestica MD01G1207800.v1.1 MD07G1278400.v1.1
prunus_persica Prupe.2G300300_v2.0.a1
pyrus_communis pycom01g21860 pycom07g25340
rosa_chinensis RchiOBHm_Chr1g0378231
rosa_laevigata RLG00000026458
rosa_multiflora Rmu_sc0038642.1_g000003
rosa_roxburghii Rroxscaffold_4G00280630
rosa_rugosa Rorug01G0407200
rosa_samantha Rh1AG422500 Rh1BG386600 Rh1CG400200 Rh1DG417800
rosa_wichuraiana Rw1G037490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 417
AccB1I GGYRCC 1 cut(s) 382
AciI CCGC 3 cut(s) 5, 111, 710
AclWI GGATC 2 cut(s) 52, 187
AcsI RAATTY 2 cut(s) 568, 724
AfaI GTAC 3 cut(s) 64, 221, 462
AgsI TTSAA 5 cut(s) 91, 271, 573, 596, 610
AjnI CCWGG 2 cut(s) 310, 394
AjuI GAANNNNNNNTTGG 2 cut(s) 144, 176
AleI CACNNNNGTG 1 cut(s) 354
AluBI AGCT 6 cut(s) 364, 391, 497, 548, 602, 715
AluI AGCT 6 cut(s) 364, 391, 497, 548, 602, 715
Alw21I GWGCWC 1 cut(s) 586
Alw26I GTCTC 1 cut(s) 653
AlwI GGATC 2 cut(s) 52, 187
Ama87I CYCGRG 2 cut(s) 554, 656
AoxI GGCC 2 cut(s) 103, 308
ApeKI GCWGC 7 cut(s) 16, 431, 599, 685, 712, 715, 718
ApoI RAATTY 2 cut(s) 568, 724
AspLEI GCGC 2 cut(s) 128, 418
AsuHPI GGTGA 2 cut(s) 437, 613
AsuNHI GCTAGC 1 cut(s) 497
AvaI CYCGRG 2 cut(s) 554, 656
BanI GGYRCC 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 485
Bbv12I GWGCWC 1 cut(s) 586
BbvI GCAGC 7 cut(s) 3, 418, 611, 697, 702, 724, 730
BccI CCATC 1 cut(s) 646
BceAI ACGGC 1 cut(s) 113
BcgI CGANNNNNNTGC 2 cut(s) 710, 744
BciT130I CCWGG 2 cut(s) 312, 396
BcoDI GTCTC 1 cut(s) 653
BfaI CTAG 3 cut(s) 498, 588, 642
BfmI CTRYAG 4 cut(s) 71, 456, 561, 716
BfoI RGCGCY 1 cut(s) 129
BglI GCCNNNNNGGC 1 cut(s) 111
BglII AGATCT 2 cut(s) 84, 652
BisI GCNGC 9 cut(s) 17, 112, 432, 600, 686, 710, 713, 716, 719
BlsI GCNGC 9 cut(s) 18, 113, 433, 601, 687, 711, 714, 717, 720
Bme1390I CCNGG 2 cut(s) 312, 396
BmeT110I CYCGRG 2 cut(s) 554, 656
BmiI GGNNCC 1 cut(s) 384
BmrFI CCNGG 2 cut(s) 312, 396
BmsI GCATC 5 cut(s) 123, 246, 418, 664, 691
BmtI GCTAGC 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 485
BpuEI CTTGAG 1 cut(s) 307
BsaI GGTCTC 1 cut(s) 653
BsaJI CCNNGG 4 cut(s) 106, 244, 311, 663
BsaXI ACNNNNNCTCC 2 cut(s) 535, 565
BseBI CCWGG 2 cut(s) 312, 396
BseDI CCNNGG 4 cut(s) 106, 244, 311, 663
BseGI GGATG 1 cut(s) 154
BseMII CTCAG 1 cut(s) 310
BseXI GCAGC 7 cut(s) 3, 418, 611, 697, 702, 724, 730
BshFI GGCC 2 cut(s) 105, 310
BshNI GGYRCC 1 cut(s) 382
BsiHKAI GWGCWC 1 cut(s) 586
BsiHKCI CYCGRG 2 cut(s) 554, 656
BsiSI CCGG 1 cut(s) 682
BslFI GGGAC 1 cut(s) 262
BsmAI GTCTC 1 cut(s) 653
BsmFI GGGAC 1 cut(s) 262
BsnI GGCC 2 cut(s) 105, 310
Bso31I GGTCTC 1 cut(s) 653
BsoBI CYCGRG 2 cut(s) 554, 656
Bsp1286I GDGCHC 1 cut(s) 586
Bsp1407I TGTACA 1 cut(s) 219
Bsp143I GATC 6 cut(s) 44, 75, 84, 179, 264, 652
BspACI CCGC 3 cut(s) 5, 111, 710
BspANI GGCC 2 cut(s) 105, 310
BspCNI CTCAG 1 cut(s) 309
BspLI GGNNCC 1 cut(s) 384
BspMAI CTGCAG 4 cut(s) 75, 460, 565, 720
BspOI GCTAGC 1 cut(s) 501
BspPI GGATC 2 cut(s) 52, 187
BspT107I GGYRCC 1 cut(s) 382
BspTNI GGTCTC 1 cut(s) 653
BsrGI TGTACA 1 cut(s) 219
BssECI CCNNGG 4 cut(s) 106, 244, 311, 663
BssMI GATC 6 cut(s) 44, 75, 84, 179, 264, 652
BssT1I CCWWGG 2 cut(s) 244, 663
Bst2UI CCWGG 2 cut(s) 312, 396
Bst4CI ACNGT 2 cut(s) 98, 465
BstAUI TGTACA 1 cut(s) 219
BstC8I GCNNGC 2 cut(s) 420, 499
BstDEI CTNAG 1 cut(s) 296
BstF5I GGATG 1 cut(s) 154
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 2 cut(s) 128, 418
BstKTI GATC 6 cut(s) 47, 78, 87, 182, 267, 655
BstMAI GTCTC 1 cut(s) 653
BstMBI GATC 6 cut(s) 44, 75, 84, 179, 264, 652
BstMWI GCNNNNNNNGC 6 cut(s) 111, 382, 428, 691, 715, 718
BstNI CCWGG 2 cut(s) 312, 396
BstNSI RCATGY 1 cut(s) 422
BstSCI CCNGG 2 cut(s) 310, 394
BstSFI CTRYAG 4 cut(s) 71, 456, 561, 716
BstV1I GCAGC 7 cut(s) 3, 418, 611, 697, 702, 724, 730
BstV2I GAAGAC 1 cut(s) 485
BstX2I RGATCY 3 cut(s) 84, 179, 652
BstYI RGATCY 3 cut(s) 84, 179, 652
BsuRI GGCC 2 cut(s) 105, 310
BtsCI GGATG 1 cut(s) 154
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 1 cut(s) 453
Cac8I GCNNGC 2 cut(s) 420, 499
CfoI GCGC 2 cut(s) 128, 418
Csp6I GTAC 3 cut(s) 63, 220, 461
CviAII CATG 2 cut(s) 419, 735
CviQI GTAC 3 cut(s) 63, 220, 461
DdeI CTNAG 1 cut(s) 296
DpnI GATC 6 cut(s) 46, 77, 86, 181, 266, 654
DpnII GATC 6 cut(s) 44, 75, 84, 179, 264, 652
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 244, 663
Eco31I GGTCTC 1 cut(s) 653
Eco88I CYCGRG 2 cut(s) 554, 656
EcoRI GAATTC 1 cut(s) 724
EcoRII CCWGG 2 cut(s) 310, 394
EcoT14I CCWWGG 2 cut(s) 244, 663
ErhI CCWWGG 2 cut(s) 244, 663
FaeI CATG 2 cut(s) 422, 738
FaqI GGGAC 1 cut(s) 262
FatI CATG 2 cut(s) 418, 734
Fnu4HI GCNGC 9 cut(s) 17, 112, 432, 600, 686, 710, 713, 716, 719
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 9 cut(s) 17, 112, 432, 600, 686, 710, 713, 716, 719
FspBI CTAG 3 cut(s) 498, 588, 642
FspI TGCGCA 1 cut(s) 417
GlaI GCGC 2 cut(s) 127, 417
GluI GCNGC 9 cut(s) 17, 112, 432, 600, 686, 710, 713, 716, 719
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 2 cut(s) 105, 310
HapII CCGG 1 cut(s) 682
HhaI GCGC 2 cut(s) 128, 418
Hin1II CATG 2 cut(s) 422, 738
Hin6I GCGC 2 cut(s) 126, 416
HinP1I GCGC 2 cut(s) 126, 416
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 1 cut(s) 557
HpaI GTTAAC 1 cut(s) 277
HpaII CCGG 1 cut(s) 682
HphI GGTGA 2 cut(s) 437, 613
Hpy166II GTNNAC 3 cut(s) 173, 222, 277
Hpy188I TCNGA 3 cut(s) 49, 652, 732
Hpy188III TCNNGA 6 cut(s) 204, 230, 286, 554, 656, 658
Hpy8I GTNNAC 3 cut(s) 173, 222, 277
HpyAV CCTTC 1 cut(s) 210
HpyCH4III ACNGT 2 cut(s) 98, 465
HpyCH4V TGCA 9 cut(s) 19, 73, 345, 446, 458, 563, 677, 704, 718
HpyF10VI GCNNNNNNNGC 6 cut(s) 111, 382, 428, 691, 715, 718
HpyF3I CTNAG 1 cut(s) 296
Hsp92II CATG 2 cut(s) 422, 738
HspAI GCGC 2 cut(s) 126, 416
KspAI GTTAAC 1 cut(s) 277
Kzo9I GATC 6 cut(s) 44, 75, 84, 179, 264, 652
LmnI GCTCC 2 cut(s) 589, 629
Lsp1109I GCAGC 7 cut(s) 3, 418, 611, 697, 702, 724, 730
LweI GCATC 5 cut(s) 123, 246, 418, 664, 691
MaeI CTAG 3 cut(s) 498, 588, 642
MaeIII GTNAC 3 cut(s) 31, 490, 529
MalI GATC 6 cut(s) 46, 77, 86, 181, 266, 654
MboI GATC 6 cut(s) 44, 75, 84, 179, 264, 652
MboII GAAGA 6 cut(s) 79, 109, 259, 490, 680, 683
MflI RGATCY 3 cut(s) 84, 179, 652
MhlI GDGCHC 1 cut(s) 586
MluCI AATT 2 cut(s) 568, 724
MlyI GAGTC 1 cut(s) 566
MnlI CCTC 4 cut(s) 116, 139, 282, 572
MseI TTAA 2 cut(s) 276, 405
MslI CAYNNNNRTG 2 cut(s) 354, 423
MspA1I CMGCKG 1 cut(s) 715
MspI CCGG 1 cut(s) 682
MspR9I CCNGG 2 cut(s) 312, 396
MvaI CCWGG 2 cut(s) 312, 396
MwoI GCNNNNNNNGC 6 cut(s) 111, 382, 428, 691, 715, 718
NdeII GATC 6 cut(s) 44, 75, 84, 179, 264, 652
NheI GCTAGC 1 cut(s) 497
NlaIII CATG 2 cut(s) 422, 738
NlaIV GGNNCC 1 cut(s) 384
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 1 cut(s) 31
NsbI TGCGCA 1 cut(s) 417
NspI RCATGY 1 cut(s) 422
OliI CACNNNNGTG 1 cut(s) 354
PaeI GCATGC 1 cut(s) 422
PaeR7I CTCGAG 1 cut(s) 656
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PkrI GCNGC 9 cut(s) 18, 113, 433, 601, 687, 711, 714, 717, 720
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
Psp6I CCWGG 2 cut(s) 310, 394
PspGI CCWGG 2 cut(s) 310, 394
PspN4I GGNNCC 1 cut(s) 384
PstI CTGCAG 4 cut(s) 75, 460, 565, 720
PsuI RGATCY 3 cut(s) 84, 179, 652
PvuII CAGCTG 1 cut(s) 715
RsaI GTAC 3 cut(s) 64, 221, 462
RsaNI GTAC 3 cut(s) 63, 220, 461
RseI CAYNNNNRTG 2 cut(s) 354, 423
SaqAI TTAA 2 cut(s) 276, 405
SatI GCNGC 9 cut(s) 17, 112, 432, 600, 686, 710, 713, 716, 719
Sau3AI GATC 6 cut(s) 44, 75, 84, 179, 264, 652
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 2 cut(s) 312, 396
SduI GDGCHC 1 cut(s) 586
SfaNI GCATC 5 cut(s) 123, 246, 418, 664, 691
SfcI CTRYAG 4 cut(s) 71, 456, 561, 716
Sfr274I CTCGAG 1 cut(s) 656
SlaI CTCGAG 1 cut(s) 656
SmiMI CAYNNNNRTG 2 cut(s) 354, 423
SmlI CTYRAG 2 cut(s) 286, 656
SmoI CTYRAG 2 cut(s) 286, 656
SphI GCATGC 1 cut(s) 422
Sse9I AATT 2 cut(s) 568, 724
SsiI CCGC 3 cut(s) 5, 111, 710
SspMI CTAG 3 cut(s) 498, 588, 642
StyD4I CCNGG 2 cut(s) 310, 394
StyI CCWWGG 2 cut(s) 244, 663
TaaI ACNGT 2 cut(s) 98, 465
TaqI TCGA 2 cut(s) 263, 657
TasI AATT 2 cut(s) 568, 724
TatI WGTACW 1 cut(s) 219
TauI GCSGC 2 cut(s) 114, 712
Tru1I TTAA 2 cut(s) 276, 405
Tru9I TTAA 2 cut(s) 276, 405
TscAI CASTG 1 cut(s) 460
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 7 cut(s) 16, 431, 599, 685, 712, 715, 718
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 1 cut(s) 165
TspRI CASTG 1 cut(s) 460
XapI RAATTY 2 cut(s) 568, 724
XceI RCATGY 1 cut(s) 422
XhoI CTCGAG 1 cut(s) 656
XspI CTAG 3 cut(s) 498, 588, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.