Rroxscaffold_4G00280630

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013336
Physical Location & Seq
Reverse (-)
3133684 .. 3137271
3588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00280630.1

Sequence Viewer

Length: 738 bp
ATGGTCATTTTGGGTTCTGTGAACAAGGAAGTGGTGTTTTTCAGTTGTCCATCAGGAAAAAATCCAATGTACACATATCGCGAATGCATCGTCCTTGGAAAAACAGATTGTTCAATCTTCAAGGTCAATCAAATCTTGAGGGAACTCAGTAGAGAGTGGCCTGGGTTTACTTATGACTTGTTATCAAAAAATTGCAATCACTTTTGTGATGAGCTATGCGAAAGACTTGGGGTGCCAAAGCTTCCAGGTTGGGTTAATCGCTTTGCACATGCGGGTGATGCTGCAATGGAAGTAGCAGGAAACACAGCAGTACGGTTTAAACAAGCCAAGACAGAGATTGTATCGGCGAGCAAAGTTGCTTATCGATTCCTTGTTGGCGTCACTAACAATGTCATAGCTTCTCCCGAAGCTCCTGGAAATTCAAATAGAGGGACCCCTAGATTTCAATCAGCTTGGTTCAAAAGCCTAATCCCTAATGGAGCCAAACCGTCTAGTAGTTCTGAATTTGATAGTGAAGAGAATGCACTTCGGCAGCAACAGCAGACAGATTCAGACTTGCCGCTACCACGACATTCTCGACCTTTGCAGCAGACAGATTCAGACTTGCTGCTACCACAGCACTCTCGACCATTGCAGCAGACAGATTCAGACTTGCCGCTACCACAACATTCTCGACCATTGCAGCAGACAGATTCAGACTTGCCGCTACCACAACATTCTCGACCATTGCATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

245

Amino Acids

27.36

Weight (kDa)

7.66

Isoelectric Point (pI)

53.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 13 - 97 5.3e-27 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013336)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25660 AT4G25680
fragaria_vesca FvH4_7g30180
malus_domestica MD01G1207800.v1.1 MD07G1278400.v1.1
prunus_persica Prupe.2G300300_v2.0.a1
pyrus_communis pycom01g21860 pycom07g25340
rosa_chinensis RchiOBHm_Chr1g0378231
rosa_laevigata RLG00000026458
rosa_multiflora Rmu_sc0038642.1_g000003
rosa_roxburghii Rroxscaffold_4G00280630
rosa_rugosa Rorug01G0407200
rosa_samantha Rh1AG422500 Rh1BG386600 Rh1CG400200 Rh1DG417800
rosa_wichuraiana Rw1G037490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 232
AccII CGCG 1 cut(s) 81
AciI CCGC 4 cut(s) 272, 560, 656, 704
AcsI RAATTY 2 cut(s) 418, 503
AcyI GRCGYC 1 cut(s) 378
AfaI GTAC 2 cut(s) 71, 312
AgsI TTSAA 5 cut(s) 114, 121, 423, 446, 460
AjnI CCWGG 3 cut(s) 160, 244, 412
AleI CACNNNNGTG 1 cut(s) 204
AluBI AGCT 5 cut(s) 214, 241, 398, 410, 452
AluI AGCT 5 cut(s) 214, 241, 398, 410, 452
AoxI GGCC 1 cut(s) 158
ApeKI GCWGC 6 cut(s) 281, 532, 586, 607, 634, 682
ApoI RAATTY 2 cut(s) 418, 503
ArsI GACNNNNNNTTYG 2 cut(s) 75, 107
AspS9I GGNCC 1 cut(s) 432
AsuHPI GGTGA 1 cut(s) 287
AvaII GGWCC 1 cut(s) 432
BanI GGYRCC 1 cut(s) 232
BbvI GCAGC 6 cut(s) 268, 544, 594, 598, 646, 694
BccI CCATC 1 cut(s) 58
BciT130I CCWGG 3 cut(s) 162, 246, 414
BfaI CTAG 2 cut(s) 438, 492
BisI GCNGC 9 cut(s) 282, 533, 560, 587, 608, 635, 656, 683, 704
BlsI GCNGC 9 cut(s) 283, 534, 561, 588, 609, 636, 657, 684, 705
Bme1390I CCNGG 3 cut(s) 162, 246, 414
Bme18I GGWCC 1 cut(s) 432
BmgT120I GGNCC 1 cut(s) 432
BmiI GGNNCC 4 cut(s) 234, 433, 434, 481
BmrFI CCNGG 3 cut(s) 162, 246, 414
BmsI GCATC 2 cut(s) 96, 268
BpuEI CTTGAG 1 cut(s) 157
Bsa29I ATCGAT 1 cut(s) 364
BsaBI GATNNNNATC 1 cut(s) 445
BsaHI GRCGYC 1 cut(s) 378
BsaJI CCNNGG 2 cut(s) 94, 161
Bse3DI GCAATG 4 cut(s) 291, 629, 677, 725
Bse8I GATNNNNATC 1 cut(s) 445
BseBI CCWGG 3 cut(s) 162, 246, 414
BseCI ATCGAT 1 cut(s) 364
BseDI CCNNGG 2 cut(s) 94, 161
BseJI GATNNNNATC 1 cut(s) 445
BseMI GCAATG 4 cut(s) 291, 629, 677, 725
BseMII CTCAG 1 cut(s) 160
BseXI GCAGC 6 cut(s) 268, 544, 594, 598, 646, 694
Bsh1236I CGCG 1 cut(s) 81
BshFI GGCC 1 cut(s) 160
BshNI GGYRCC 1 cut(s) 232
BshVI ATCGAT 1 cut(s) 364
BslFI GGGAC 1 cut(s) 445
BsmFI GGGAC 1 cut(s) 445
BsmI GAATGC 2 cut(s) 89, 526
BsnI GGCC 1 cut(s) 160
Bsp1407I TGTACA 1 cut(s) 69
Bsp68I TCGCGA 1 cut(s) 81
BspACI CCGC 4 cut(s) 272, 560, 656, 704
BspANI GGCC 1 cut(s) 160
BspCNI CTCAG 1 cut(s) 159
BspDI ATCGAT 1 cut(s) 364
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 4 cut(s) 234, 433, 434, 481
BspT107I GGYRCC 1 cut(s) 232
BsrDI GCAATG 4 cut(s) 291, 629, 677, 725
BsrGI TGTACA 1 cut(s) 69
BssECI CCNNGG 2 cut(s) 94, 161
BssNI GRCGYC 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 94
Bst2UI CCWGG 3 cut(s) 162, 246, 414
Bst4CI ACNGT 2 cut(s) 315, 489
Bst6I CTCTTC 1 cut(s) 510
BstACI GRCGYC 1 cut(s) 378
BstAUI TGTACA 1 cut(s) 69
BstC8I GCNNGC 1 cut(s) 349
BstDEI CTNAG 1 cut(s) 146
BstFNI CGCG 1 cut(s) 81
BstMWI GCNNNNNNNGC 3 cut(s) 278, 538, 616
BstNI CCWGG 3 cut(s) 162, 246, 414
BstNSI RCATGY 1 cut(s) 272
BstSCI CCNGG 3 cut(s) 160, 244, 412
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 6 cut(s) 268, 544, 594, 598, 646, 694
Bsu15I ATCGAT 1 cut(s) 364
BsuRI GGCC 1 cut(s) 160
BsuTUI ATCGAT 1 cut(s) 364
BtuMI TCGCGA 1 cut(s) 81
Cac8I GCNNGC 1 cut(s) 349
Cfr13I GGNCC 1 cut(s) 432
ClaI ATCGAT 1 cut(s) 364
CseI GACGC 1 cut(s) 367
Csp6I GTAC 2 cut(s) 70, 311
CviAII CATG 1 cut(s) 269
CviJI RGCY 9 cut(s) 160, 214, 241, 326, 398, 410, 452, 465, 482
CviKI_1 RGCY 9 cut(s) 160, 214, 241, 326, 398, 410, 452, 465, 482
CviQI GTAC 2 cut(s) 70, 311
DdeI CTNAG 1 cut(s) 146
DraI TTTAAA 1 cut(s) 319
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
Eco130I CCWWGG 1 cut(s) 94
Eco47I GGWCC 1 cut(s) 432
EcoO109I RGGNCCY 1 cut(s) 432
EcoRII CCWGG 3 cut(s) 160, 244, 412
EcoT14I CCWWGG 1 cut(s) 94
EcoT22I ATGCAT 1 cut(s) 89
ErhI CCWWGG 1 cut(s) 94
FaeI CATG 1 cut(s) 272
FaiI YATR 6 cut(s) 76, 174, 217, 270, 395, 732
FaqI GGGAC 1 cut(s) 445
FatI CATG 1 cut(s) 268
FauI CCCGC 1 cut(s) 265
Fnu4HI GCNGC 9 cut(s) 282, 533, 560, 587, 608, 635, 656, 683, 704
Fsp4HI GCNGC 9 cut(s) 282, 533, 560, 587, 608, 635, 656, 683, 704
FspBI CTAG 2 cut(s) 438, 492
GluI GCNGC 9 cut(s) 282, 533, 560, 587, 608, 635, 656, 683, 704
HaeIII GGCC 1 cut(s) 160
HgaI GACGC 1 cut(s) 367
Hin1I GRCGYC 1 cut(s) 378
Hin1II CATG 1 cut(s) 272
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 5 cut(s) 366, 548, 596, 644, 692
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 3 cut(s) 22, 72, 168
Hpy188I TCNGA 5 cut(s) 502, 553, 601, 649, 697
Hpy188III TCNNGA 8 cut(s) 54, 80, 136, 404, 576, 624, 672, 720
Hpy8I GTNNAC 3 cut(s) 22, 72, 168
HpyCH4III ACNGT 2 cut(s) 315, 489
HpyCH4V TGCA 9 cut(s) 87, 195, 266, 284, 524, 586, 634, 682, 730
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 538, 616
HpyF3I CTNAG 1 cut(s) 146
Hsp92I GRCGYC 1 cut(s) 378
Hsp92II CATG 1 cut(s) 272
KflI GGGWCCC 1 cut(s) 432
LmnI GCTCC 2 cut(s) 415, 479
LpnPI CCDG 8 cut(s) 39, 147, 174, 231, 258, 282, 399, 426
Lsp1109I GCAGC 6 cut(s) 268, 544, 594, 598, 646, 694
LweI GCATC 2 cut(s) 96, 268
MaeI CTAG 2 cut(s) 438, 492
MaeIII GTNAC 1 cut(s) 379
MboII GAAGA 2 cut(s) 109, 527
MluCI AATT 3 cut(s) 190, 418, 503
MnlI CCTC 2 cut(s) 132, 422
Mph1103I ATGCAT 1 cut(s) 89
MseI TTAA 2 cut(s) 255, 318
MslI CAYNNNNRTG 2 cut(s) 204, 273
MspR9I CCNGG 3 cut(s) 162, 246, 414
MssI GTTTAAAC 1 cut(s) 319
Mva1269I GAATGC 2 cut(s) 89, 526
MvaI CCWGG 3 cut(s) 162, 246, 414
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 3 cut(s) 278, 538, 616
NlaIII CATG 1 cut(s) 272
NlaIV GGNNCC 4 cut(s) 234, 433, 434, 481
NmuCI GTSAC 1 cut(s) 379
NruI TCGCGA 1 cut(s) 81
NsiI ATGCAT 1 cut(s) 89
NspI RCATGY 1 cut(s) 272
OliI CACNNNNGTG 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 574
PctI GAATGC 2 cut(s) 89, 526
PfeI GAWTC 5 cut(s) 366, 548, 596, 644, 692
PfoI TCCNGGA 1 cut(s) 412
PkrI GCNGC 9 cut(s) 283, 534, 561, 588, 609, 636, 657, 684, 705
PmeI GTTTAAAC 1 cut(s) 319
PpuMI RGGWCCY 1 cut(s) 432
Psp5II RGGWCCY 1 cut(s) 432
Psp6I CCWGG 3 cut(s) 160, 244, 412
PspGI CCWGG 3 cut(s) 160, 244, 412
PspN4I GGNNCC 4 cut(s) 234, 433, 434, 481
PspPI GGNCC 1 cut(s) 432
PspPPI RGGWCCY 1 cut(s) 432
RruI TCGCGA 1 cut(s) 81
RsaI GTAC 2 cut(s) 71, 312
RsaNI GTAC 2 cut(s) 70, 311
RseI CAYNNNNRTG 2 cut(s) 204, 273
SaqAI TTAA 2 cut(s) 255, 318
SatI GCNGC 9 cut(s) 282, 533, 560, 587, 608, 635, 656, 683, 704
Sau96I GGNCC 1 cut(s) 432
ScrFI CCNGG 3 cut(s) 162, 246, 414
SetI ASST 8 cut(s) 126, 216, 243, 250, 400, 412, 454, 583
SfaNI GCATC 2 cut(s) 96, 268
SinI GGWCC 1 cut(s) 432
SmiMI CAYNNNNRTG 2 cut(s) 204, 273
SmlI CTYRAG 1 cut(s) 136
SmoI CTYRAG 1 cut(s) 136
Sse9I AATT 3 cut(s) 190, 418, 503
SsiI CCGC 4 cut(s) 272, 560, 656, 704
SspMI CTAG 2 cut(s) 438, 492
StyD4I CCNGG 3 cut(s) 160, 244, 412
StyI CCWWGG 1 cut(s) 94
TaaI ACNGT 2 cut(s) 315, 489
TaqI TCGA 5 cut(s) 364, 577, 625, 673, 721
TasI AATT 3 cut(s) 190, 418, 503
TatI WGTACW 1 cut(s) 69
TauI GCSGC 3 cut(s) 562, 658, 706
TfiI GAWTC 5 cut(s) 366, 548, 596, 644, 692
Tru1I TTAA 2 cut(s) 255, 318
Tru9I TTAA 2 cut(s) 255, 318
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 6 cut(s) 281, 532, 586, 607, 634, 682
Tsp45I GTSAC 1 cut(s) 379
VpaK11BI GGWCC 1 cut(s) 432
XapI RAATTY 2 cut(s) 418, 503
XceI RCATGY 1 cut(s) 272
XspI CTAG 2 cut(s) 438, 492
Zsp2I ATGCAT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.