pycom01g21860

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0013336
Physical Location & Seq
Forward (+)
19691737 .. 19693687
1951 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21860.1

Sequence Viewer

Length: 750 bp
ATGGCGGAGGTGGTGCTGCATATATACGATGTGACGAATAGTGGATCGAACAAGACGAATAGCACCATTCTGCAGATCAACAAGATCTTCAAGGATGGTATCGGCCTCGGTGGCATCTTCCACAGCGCCGTTCAGGTTTATGGAGATGATGAATGGTCATTTGGGTTCTGTGAACAAGGATCTGGTGTGTTTAGTTGCCCTTCTGGGAAAAATCCAATGTACACATATCGTGAAAGCATCGTTCTTGGGACAACGCATTGTTCGATCTTCAAGGTTAACCAAATCTTGAGGGAACTCAGTAGAGAGTGGCCAGGGTATTCGTATGACCTGTTATCAAAAAATTGCAATCACTTTTGTGATGAGTTATGTGAAAGGCTTGGGGTGCCAAGGCTTCCAGGTTGGGTTAATCGTTTTGCCCATGCTGGTGATGCTGCTATGGAAGTCGCAGGAAACACGGCAGAACGGTTTAGAGAAGCGAAGACACAGATTGTTTCAGCTAGCAAAGTAGCATATCGTTTCCTTGTGGGTGTTACAAGCAATGCCATAGCTTCTCCCGCATCTCCTGAAAATTCAGACAGAGGTACTCCTAGATTTCAGGCCGCATGGTTTAAGAATCTAATCACAACGGGAGCAAAGCCTTCTAGTAGTTCAGATTTCGAGAATAAGGAAGAGGATGTACTTCGGCAGAACCAGCAACCGGATGCAGAGCCGCCGCTGCAGCATAATTCTCGAACATGGCAAAGTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

250

Amino Acids

27.61

Weight (kDa)

6.08

Isoelectric Point (pI)

31.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 3 - 149 1.6e-42 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013336)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25660 AT4G25680
fragaria_vesca FvH4_7g30180
malus_domestica MD01G1207800.v1.1 MD07G1278400.v1.1
prunus_persica Prupe.2G300300_v2.0.a1
pyrus_communis pycom01g21860 pycom07g25340
rosa_chinensis RchiOBHm_Chr1g0378231
rosa_laevigata RLG00000026458
rosa_multiflora Rmu_sc0038642.1_g000003
rosa_roxburghii Rroxscaffold_4G00280630
rosa_rugosa Rorug01G0407200
rosa_samantha Rh1AG422500 Rh1BG386600 Rh1CG400200 Rh1DG417800
rosa_wichuraiana Rw1G037490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 382
AciI CCGC 5 cut(s) 5, 555, 600, 710, 713
AclWI GGATC 2 cut(s) 52, 187
AcoI YGGCCR 1 cut(s) 308
AcsI RAATTY 1 cut(s) 568
AfaI GTAC 4 cut(s) 221, 583, 678, 745
AfiI CCNNNNNNNGG 1 cut(s) 697
AgsI TTSAA 2 cut(s) 91, 271
AjnI CCWGG 2 cut(s) 310, 394
AjuI GAANNNNNNNTTGG 2 cut(s) 144, 176
AleI CACNNNNGTG 1 cut(s) 354
AluBI AGCT 2 cut(s) 497, 548
AluI AGCT 2 cut(s) 497, 548
AlwI GGATC 2 cut(s) 52, 187
AoxI GGCC 3 cut(s) 103, 308, 597
ApeKI GCWGC 4 cut(s) 16, 431, 715, 718
ApoI RAATTY 1 cut(s) 568
AspLEI GCGC 1 cut(s) 128
AsuHPI GGTGA 1 cut(s) 437
AsuNHI GCTAGC 1 cut(s) 497
BalI TGGCCA 1 cut(s) 310
BanI GGYRCC 1 cut(s) 382
BarI GAAGNNNNNNTAC 2 cut(s) 660, 692
BbsI GAAGAC 1 cut(s) 485
BbvI GCAGC 4 cut(s) 3, 418, 702, 730
BccI CCATC 1 cut(s) 89
BceAI ACGGC 2 cut(s) 113, 471
BcgI CGANNNNNNTGC 2 cut(s) 710, 744
BciT130I CCWGG 2 cut(s) 312, 396
BfaI CTAG 3 cut(s) 498, 588, 642
BfmI CTRYAG 2 cut(s) 71, 716
BfoI RGCGCY 1 cut(s) 129
BglI GCCNNNNNGGC 1 cut(s) 111
BglII AGATCT 1 cut(s) 84
BisI GCNGC 7 cut(s) 17, 432, 600, 710, 713, 716, 719
BlsI GCNGC 7 cut(s) 18, 433, 601, 711, 714, 717, 720
Bme1390I CCNGG 2 cut(s) 312, 396
BmiI GGNNCC 1 cut(s) 384
BmrFI CCNGG 2 cut(s) 312, 396
BmsI GCATC 5 cut(s) 123, 246, 418, 566, 691
BmtI GCTAGC 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 485
BpuEI CTTGAG 1 cut(s) 307
BsaAI YACGTR 1 cut(s) 747
BsaJI CCNNGG 3 cut(s) 106, 311, 386
BsaWI WCCGGW 1 cut(s) 697
Bsc4I CCNNNNNNNGG 1 cut(s) 697
Bse3DI GCAATG 1 cut(s) 544
BseBI CCWGG 2 cut(s) 312, 396
BseDI CCNNGG 3 cut(s) 106, 311, 386
BseGI GGATG 3 cut(s) 100, 679, 706
BseLI CCNNNNNNNGG 1 cut(s) 697
BseMI GCAATG 1 cut(s) 544
BseMII CTCAG 1 cut(s) 310
BseXI GCAGC 4 cut(s) 3, 418, 702, 730
BshFI GGCC 3 cut(s) 105, 310, 599
BshNI GGYRCC 1 cut(s) 382
BsiSI CCGG 1 cut(s) 698
BslFI GGGAC 1 cut(s) 262
BslI CCNNNNNNNGG 1 cut(s) 697
BsmFI GGGAC 1 cut(s) 262
BsnI GGCC 3 cut(s) 105, 310, 599
Bsp1407I TGTACA 1 cut(s) 219
Bsp143I GATC 5 cut(s) 44, 75, 84, 179, 264
BspACI CCGC 5 cut(s) 5, 555, 600, 710, 713
BspANI GGCC 3 cut(s) 105, 310, 599
BspCNI CTCAG 1 cut(s) 309
BspLI GGNNCC 1 cut(s) 384
BspMAI CTGCAG 2 cut(s) 75, 720
BspOI GCTAGC 1 cut(s) 501
BspPI GGATC 2 cut(s) 52, 187
BspT107I GGYRCC 1 cut(s) 382
BsrDI GCAATG 1 cut(s) 544
BsrGI TGTACA 1 cut(s) 219
BssECI CCNNGG 3 cut(s) 106, 311, 386
BssMI GATC 5 cut(s) 44, 75, 84, 179, 264
BssT1I CCWWGG 1 cut(s) 386
Bst2UI CCWGG 2 cut(s) 312, 396
Bst4CI ACNGT 1 cut(s) 465
Bst6I CTCTTC 1 cut(s) 663
BstAUI TGTACA 1 cut(s) 219
BstBAI YACGTR 1 cut(s) 747
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 1 cut(s) 296
BstF5I GGATG 3 cut(s) 100, 679, 706
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 5 cut(s) 47, 78, 87, 182, 267
BstMBI GATC 5 cut(s) 44, 75, 84, 179, 264
BstMWI GCNNNNNNNGC 7 cut(s) 111, 382, 428, 554, 691, 715, 718
BstNI CCWGG 2 cut(s) 312, 396
BstSCI CCNGG 2 cut(s) 310, 394
BstSFI CTRYAG 2 cut(s) 71, 716
BstSNI TACGTA 1 cut(s) 747
BstV1I GCAGC 4 cut(s) 3, 418, 702, 730
BstV2I GAAGAC 1 cut(s) 485
BstX2I RGATCY 2 cut(s) 84, 179
BstYI RGATCY 2 cut(s) 84, 179
BsuRI GGCC 3 cut(s) 105, 310, 599
BtsCI GGATG 3 cut(s) 100, 679, 706
Cac8I GCNNGC 1 cut(s) 499
CfoI GCGC 1 cut(s) 128
Csp6I GTAC 4 cut(s) 220, 582, 677, 744
CviAII CATG 3 cut(s) 419, 603, 735
CviJI RGCY 9 cut(s) 105, 310, 376, 391, 497, 548, 599, 637, 709
CviKI_1 RGCY 9 cut(s) 105, 310, 376, 391, 497, 548, 599, 637, 709
CviQI GTAC 4 cut(s) 220, 582, 677, 744
DdeI CTNAG 1 cut(s) 296
DpnI GATC 5 cut(s) 46, 77, 86, 181, 266
DpnII GATC 5 cut(s) 44, 75, 84, 179, 264
EaeI YGGCCR 1 cut(s) 308
Eam1104I CTCTTC 1 cut(s) 663
EarI CTCTTC 1 cut(s) 663
EciI GGCGGA 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 747
Eco130I CCWWGG 1 cut(s) 386
EcoRII CCWGG 2 cut(s) 310, 394
EcoT14I CCWWGG 1 cut(s) 386
ErhI CCWWGG 1 cut(s) 386
FaeI CATG 3 cut(s) 422, 606, 738
FaqI GGGAC 1 cut(s) 262
FatI CATG 3 cut(s) 418, 602, 734
FauI CCCGC 1 cut(s) 562
Fnu4HI GCNGC 7 cut(s) 17, 432, 600, 710, 713, 716, 719
FokI GGATG 3 cut(s) 107, 686, 713
Fsp4HI GCNGC 7 cut(s) 17, 432, 600, 710, 713, 716, 719
FspBI CTAG 3 cut(s) 498, 588, 642
GlaI GCGC 1 cut(s) 127
GluI GCNGC 7 cut(s) 17, 432, 600, 710, 713, 716, 719
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 3 cut(s) 105, 310, 599
HapII CCGG 1 cut(s) 698
HhaI GCGC 1 cut(s) 128
Hin1II CATG 3 cut(s) 422, 606, 738
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 1 cut(s) 613
HpaI GTTAAC 1 cut(s) 277
HpaII CCGG 1 cut(s) 698
HphI GGTGA 1 cut(s) 437
Hpy166II GTNNAC 3 cut(s) 173, 222, 277
Hpy188I TCNGA 2 cut(s) 574, 652
Hpy188III TCNNGA 5 cut(s) 230, 286, 563, 658, 729
Hpy8I GTNNAC 3 cut(s) 173, 222, 277
HpyAV CCTTC 2 cut(s) 210, 648
HpyCH4III ACNGT 1 cut(s) 465
HpyCH4IV ACGT 1 cut(s) 746
HpyCH4V TGCA 5 cut(s) 19, 73, 345, 704, 718
HpyF10VI GCNNNNNNNGC 7 cut(s) 111, 382, 428, 554, 691, 715, 718
HpyF3I CTNAG 1 cut(s) 296
HpySE526I ACGT 1 cut(s) 746
Hsp92II CATG 3 cut(s) 422, 606, 738
HspAI GCGC 1 cut(s) 126
KspAI GTTAAC 1 cut(s) 277
Kzo9I GATC 5 cut(s) 44, 75, 84, 179, 264
LmnI GCTCC 1 cut(s) 629
Lsp1109I GCAGC 4 cut(s) 3, 418, 702, 730
LweI GCATC 5 cut(s) 123, 246, 418, 566, 691
MaeI CTAG 3 cut(s) 498, 588, 642
MaeII ACGT 1 cut(s) 746
MaeIII GTNAC 2 cut(s) 31, 529
MalI GATC 5 cut(s) 46, 77, 86, 181, 266
MboI GATC 5 cut(s) 44, 75, 84, 179, 264
MboII GAAGA 5 cut(s) 79, 109, 259, 490, 680
MflI RGATCY 2 cut(s) 84, 179
MlsI TGGCCA 1 cut(s) 310
MluCI AATT 3 cut(s) 340, 568, 724
MluNI TGGCCA 1 cut(s) 310
MnlI CCTC 4 cut(s) 116, 282, 572, 664
Mox20I TGGCCA 1 cut(s) 310
MscI TGGCCA 1 cut(s) 310
MseI TTAA 3 cut(s) 276, 405, 609
MslI CAYNNNNRTG 2 cut(s) 354, 423
Msp20I TGGCCA 1 cut(s) 310
MspA1I CMGCKG 1 cut(s) 715
MspI CCGG 1 cut(s) 698
MspR9I CCNGG 2 cut(s) 312, 396
MvaI CCWGG 2 cut(s) 312, 396
MwoI GCNNNNNNNGC 7 cut(s) 111, 382, 428, 554, 691, 715, 718
NdeII GATC 5 cut(s) 44, 75, 84, 179, 264
NheI GCTAGC 1 cut(s) 497
NlaIII CATG 3 cut(s) 422, 606, 738
NlaIV GGNNCC 1 cut(s) 384
NmuCI GTSAC 1 cut(s) 31
OliI CACNNNNGTG 1 cut(s) 354
PcsI WCGNNNNNNNCGW 2 cut(s) 53, 260
PfeI GAWTC 1 cut(s) 613
PkrI GCNGC 7 cut(s) 18, 433, 601, 711, 714, 717, 720
Ppu21I YACGTR 1 cut(s) 747
Psp6I CCWGG 2 cut(s) 310, 394
PspGI CCWGG 2 cut(s) 310, 394
PspN4I GGNNCC 1 cut(s) 384
PstI CTGCAG 2 cut(s) 75, 720
PsuI RGATCY 2 cut(s) 84, 179
RsaI GTAC 4 cut(s) 221, 583, 678, 745
RsaNI GTAC 4 cut(s) 220, 582, 677, 744
RseI CAYNNNNRTG 2 cut(s) 354, 423
SaqAI TTAA 3 cut(s) 276, 405, 609
SatI GCNGC 7 cut(s) 17, 432, 600, 710, 713, 716, 719
Sau3AI GATC 5 cut(s) 44, 75, 84, 179, 264
ScrFI CCNGG 2 cut(s) 312, 396
SetI ASST 9 cut(s) 12, 138, 276, 330, 400, 499, 550, 583, 749
SfaNI GCATC 5 cut(s) 123, 246, 418, 566, 691
SfcI CTRYAG 2 cut(s) 71, 716
SmiMI CAYNNNNRTG 2 cut(s) 354, 423
SmlI CTYRAG 1 cut(s) 286
SmoI CTYRAG 1 cut(s) 286
SnaBI TACGTA 1 cut(s) 747
Sse9I AATT 3 cut(s) 340, 568, 724
SsiI CCGC 5 cut(s) 5, 555, 600, 710, 713
SspMI CTAG 3 cut(s) 498, 588, 642
StyD4I CCNGG 2 cut(s) 310, 394
StyI CCWWGG 1 cut(s) 386
TaaI ACNGT 1 cut(s) 465
TaiI ACGT 1 cut(s) 749
TaqI TCGA 4 cut(s) 47, 263, 657, 730
TasI AATT 3 cut(s) 340, 568, 724
TatI WGTACW 2 cut(s) 219, 676
TauI GCSGC 3 cut(s) 602, 712, 715
TfiI GAWTC 1 cut(s) 613
Tru1I TTAA 3 cut(s) 276, 405, 609
Tru9I TTAA 3 cut(s) 276, 405, 609
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 4 cut(s) 16, 431, 715, 718
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 1 cut(s) 165
XapI RAATTY 1 cut(s) 568
XspI CTAG 3 cut(s) 498, 588, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.