MD08G1012900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012583
Physical Location & Seq
Reverse (-)
959802 .. 961361
1560 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1012900.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGAACAGAGAGAAACACTAACAATGGAACAGAGGCTTCTACTCTTCCCCTGTTTTCTGAACGCCAGCGACGCCGAGATAGAGGTGGCGAAAATCCTGATTCATCTTCCCCACATGATCTCCGAATTCGAAACTCCGCTTCCAATTCCGCTCGCGCTCGTCTGGGGATGCAAGGGAAAGAGATCTGCACTCGCTCTGAAGAACGACGGCGGCGCTTCCACTCTGCGTCGTCTGATTGGACCTGATTTTTTGTCTTCACTGCCACTCTGGGCTTCTCTGGCTCCTCGGCCTTTGGCGGCCAAGGCTCCGCCGGCTCCCCCCGTCCCTAATGCTGTCAAGGAGGAGACTTCCAGCCCCGATACGCCGCTGCGCTTCGAGCCCAGTGAATCTGATGAGAAGCAGCCTGACCACTTGAGAAGTAAACTCCTTGGTCAAAAAAGGAAGACAGAGGAATGGATTACAATTGTGGACGAATTGACTTCGAAGAAAAAATTGCGAAATCAAGAGCTTGAGAATGTGAAGCGTTTCTTTGACAAGATGGATGGTCTTAATTTGGAGTTGAAAGCAAGGAAAGAGGAGCTAATGAGACTTGGCCATAAACTGGAAGAGGAGCAGCGTTTGGATACGGACCCAAGCCCGGAACCAACTGTGCAATTAAGCCAACCGACCCAACTCACGTTTGTGACTCCGCCTCAAGCGGCCCCAAATCAAGAGCCTTACCAACATCCAATTCCAATGCAAATGCAAATTCCCTCTCCTCCTAGTGTGATTTTTTATCAACATCAACTGCAGCAGCTGATCATGGATCAAACGGCCAAAAATCAAAGAAGAGAGTTCAATGTGGGCCCGCGAGGGGATCACGTTAACCTCTGTTTTAAGGATTCAATCGTGTTGAACTCCTCCCAGCCGTTTGATCTTGGCTTGGTTAACAAGGATATGATTAGGAAAAGGGCTGCTGAGGCAAGGCAGAAGAGGCTCGAAATCTGTAGGGTCAAGAAATCTCGCTTCCACTCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

340

Amino Acids

38.7

Weight (kDa)

8.94

Isoelectric Point (pI)

59.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 601
AccBSI CCGCTC 1 cut(s) 151
AccII CGCG 2 cut(s) 155, 850
AciI CCGC 9 cut(s) 137, 149, 210, 296, 308, 365, 689, 698, 848
AclWI GGATC 2 cut(s) 813, 864
AcoI YGGCCR 3 cut(s) 297, 592, 813
AcsI RAATTY 2 cut(s) 125, 747
AcuI CTGAAG 1 cut(s) 218
AcyI GRCGYC 1 cut(s) 72
AfiI CCNNNNNNNGG 3 cut(s) 601, 637, 853
AgsI TTSAA 4 cut(s) 562, 838, 885, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 714, 746
AleI CACNNNNGTG 1 cut(s) 680
AluBI AGCT 3 cut(s) 508, 580, 796
AluI AGCT 3 cut(s) 508, 580, 796
Alw26I GTCTC 2 cut(s) 338, 580
AlwI GGATC 2 cut(s) 813, 864
AlwNI CAGNNNCTG 1 cut(s) 796
AoxI GGCC 6 cut(s) 287, 297, 592, 699, 813, 844
ApaI GGGCCC 1 cut(s) 848
ApeKI GCWGC 6 cut(s) 367, 400, 613, 790, 793, 953
ApoI RAATTY 2 cut(s) 125, 747
Asp700I GAANNNNTTC 1 cut(s) 524
AspLEI GCGC 3 cut(s) 157, 215, 372
AspS9I GGNCC 5 cut(s) 239, 628, 700, 844, 845
AsuC2I CCSGG 1 cut(s) 638
AsuII TTCGAA 2 cut(s) 129, 482
AvaII GGWCC 2 cut(s) 239, 628
BaeGI GKGCMC 1 cut(s) 848
BalI TGGCCA 1 cut(s) 594
BanII GRGCYC 2 cut(s) 381, 848
BbsI GAAGAC 2 cut(s) 246, 449
BbvCI CCTCAGC 1 cut(s) 957
BbvI GCAGC 6 cut(s) 354, 412, 625, 802, 805, 940
BccI CCATC 2 cut(s) 532, 536
BceAI ACGGC 3 cut(s) 223, 828, 892
BciVI GTATCC 1 cut(s) 616
BclI TGATCA 1 cut(s) 798
BcnI CCSGG 1 cut(s) 638
BcoDI GTCTC 2 cut(s) 338, 580
BfaI CTAG 1 cut(s) 762
BfmI CTRYAG 2 cut(s) 788, 985
BfoI RGCGCY 1 cut(s) 216
BfuI GTATCC 1 cut(s) 616
BglII AGATCT 1 cut(s) 182
Bme1390I CCNGG 1 cut(s) 638
Bme18I GGWCC 2 cut(s) 239, 628
BmgT120I GGNCC 5 cut(s) 239, 628, 700, 844, 845
BmiI GGNNCC 7 cut(s) 282, 306, 315, 630, 642, 702, 846
BmrFI CCNGG 1 cut(s) 638
BmrI ACTGGG 1 cut(s) 375
BmsI GCATC 1 cut(s) 158
BmuI ACTGGG 1 cut(s) 375
BpiI GAAGAC 2 cut(s) 246, 449
BpmI CTGGAG 1 cut(s) 997
Bpu10I CCTNAGC 1 cut(s) 957
Bpu14I TTCGAA 2 cut(s) 129, 482
BpuEI CTTGAG 3 cut(s) 433, 530, 678
BpuMI CCSGG 1 cut(s) 638
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 3 cut(s) 284, 300, 427
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 3 cut(s) 601, 637, 853
Bse118I RCCGGY 1 cut(s) 310
Bse1I ACTGG 2 cut(s) 381, 606
BseDI CCNNGG 3 cut(s) 284, 300, 427
BseGI GGATG 3 cut(s) 173, 547, 724
BseLI CCNNNNNNNGG 3 cut(s) 601, 637, 853
BseMII CTCAG 1 cut(s) 948
BseNI ACTGG 2 cut(s) 381, 606
BseRI GAGGAG 6 cut(s) 273, 356, 590, 623, 747, 889
BseSI GKGCMC 1 cut(s) 848
BseXI GCAGC 6 cut(s) 354, 412, 625, 802, 805, 940
BseYI CCCAGC 1 cut(s) 903
BsgI GTGCAG 1 cut(s) 171
Bsh1236I CGCG 2 cut(s) 155, 850
BshFI GGCC 6 cut(s) 289, 299, 594, 701, 815, 846
BsiSI CCGG 2 cut(s) 311, 638
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 3 cut(s) 601, 637, 853
BsmAI GTCTC 2 cut(s) 338, 580
BsmFI GGGAC 1 cut(s) 308
BsnI GGCC 6 cut(s) 289, 299, 594, 701, 815, 846
Bsp119I TTCGAA 2 cut(s) 129, 482
Bsp120I GGGCCC 1 cut(s) 844
Bsp1286I GDGCHC 2 cut(s) 381, 848
Bsp143I GATC 6 cut(s) 117, 182, 798, 805, 856, 913
BspACI CCGC 9 cut(s) 137, 149, 210, 296, 308, 365, 689, 698, 848
BspANI GGCC 6 cut(s) 289, 299, 594, 701, 815, 846
BspCNI CTCAG 1 cut(s) 949
BspFNI CGCG 2 cut(s) 155, 850
BspLI GGNNCC 7 cut(s) 282, 306, 315, 630, 642, 702, 846
BspMAI CTGCAG 1 cut(s) 792
BspPI GGATC 2 cut(s) 813, 864
BspT104I TTCGAA 2 cut(s) 129, 482
BsrBI CCGCTC 1 cut(s) 151
BsrFI RCCGGY 1 cut(s) 310
BsrI ACTGG 2 cut(s) 381, 606
BssAI RCCGGY 1 cut(s) 310
BssECI CCNNGG 3 cut(s) 284, 300, 427
BssMI GATC 6 cut(s) 117, 182, 798, 805, 856, 913
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 2 cut(s) 300, 427
Bst4CI ACNGT 1 cut(s) 649
Bst6I CTCTTC 4 cut(s) 50, 600, 823, 965
BstACI GRCGYC 1 cut(s) 72
BstBI TTCGAA 2 cut(s) 129, 482
BstC8I GCNNGC 4 cut(s) 67, 153, 312, 848
BstDEI CTNAG 1 cut(s) 957
BstF5I GGATG 3 cut(s) 173, 547, 724
BstFNI CGCG 2 cut(s) 155, 850
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 3 cut(s) 157, 215, 372
BstKTI GATC 6 cut(s) 120, 185, 801, 808, 859, 916
BstMAI GTCTC 2 cut(s) 338, 580
BstMBI GATC 6 cut(s) 117, 182, 798, 805, 856, 913
BstMWI GCNNNNNNNGC 7 cut(s) 71, 278, 302, 311, 376, 959, 973
BstSCI CCNGG 1 cut(s) 636
BstSFI CTRYAG 2 cut(s) 788, 985
BstSLI GKGCMC 1 cut(s) 848
BstUI CGCG 2 cut(s) 155, 850
BstV1I GCAGC 6 cut(s) 354, 412, 625, 802, 805, 940
BstV2I GAAGAC 2 cut(s) 246, 449
BstX2I RGATCY 1 cut(s) 182
BstYI RGATCY 1 cut(s) 182
BsuI GTATCC 1 cut(s) 616
BsuRI GGCC 6 cut(s) 289, 299, 594, 701, 815, 846
BtsCI GGATG 3 cut(s) 173, 547, 724
BtsI GCAGTG 1 cut(s) 257
BtsIMutI CAGTG 2 cut(s) 257, 388
Cac8I GCNNGC 4 cut(s) 67, 153, 312, 848
CaiI CAGNNNCTG 1 cut(s) 796
CfoI GCGC 3 cut(s) 157, 215, 372
Cfr10I RCCGGY 1 cut(s) 310
Cfr13I GGNCC 5 cut(s) 239, 628, 700, 844, 845
CseI GACGC 2 cut(s) 80, 215
CviAII CATG 2 cut(s) 115, 802
DdeI CTNAG 1 cut(s) 957
DpnI GATC 6 cut(s) 119, 184, 800, 807, 858, 915
DpnII GATC 6 cut(s) 117, 182, 798, 805, 856, 913
EaeI YGGCCR 3 cut(s) 297, 592, 813
Eam1104I CTCTTC 4 cut(s) 50, 600, 823, 965
EarI CTCTTC 4 cut(s) 50, 600, 823, 965
EciI GGCGGA 2 cut(s) 297, 678
Eco130I CCWWGG 2 cut(s) 300, 427
Eco24I GRGCYC 2 cut(s) 381, 848
Eco47I GGWCC 2 cut(s) 239, 628
Eco57I CTGAAG 1 cut(s) 218
EcoRI GAATTC 1 cut(s) 125
EcoT14I CCWWGG 2 cut(s) 300, 427
EcoT38I GRGCYC 2 cut(s) 381, 848
ErhI CCWWGG 2 cut(s) 300, 427
FaeI CATG 2 cut(s) 118, 805
FaiI YATR 4 cut(s) 116, 597, 803, 938
FalI AAGNNNNNCTT 2 cut(s) 512, 544
FaqI GGGAC 1 cut(s) 308
FatI CATG 2 cut(s) 114, 801
FauI CCCGC 1 cut(s) 855
FbaI TGATCA 1 cut(s) 798
FokI GGATG 3 cut(s) 180, 554, 711
FriOI GRGCYC 2 cut(s) 381, 848
FspBI CTAG 1 cut(s) 762
GlaI GCGC 3 cut(s) 156, 214, 371
GsaI CCCAGC 1 cut(s) 907
GsuI CTGGAG 1 cut(s) 997
HaeII RGCGCY 1 cut(s) 216
HaeIII GGCC 6 cut(s) 289, 299, 594, 701, 815, 846
HapII CCGG 2 cut(s) 311, 638
HgaI GACGC 2 cut(s) 80, 215
HhaI GCGC 3 cut(s) 157, 215, 372
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 2 cut(s) 118, 805
Hin6I GCGC 3 cut(s) 155, 213, 370
HinP1I GCGC 3 cut(s) 155, 213, 370
HincII GTYRAC 2 cut(s) 865, 928
HindII GTYRAC 2 cut(s) 865, 928
HinfI GANTC 4 cut(s) 100, 386, 685, 881
HpaI GTTAAC 2 cut(s) 865, 928
HpaII CCGG 2 cut(s) 311, 638
Hpy166II GTNNAC 4 cut(s) 422, 469, 865, 928
Hpy188I TCNGA 5 cut(s) 60, 124, 198, 234, 391
Hpy188III TCNNGA 5 cut(s) 97, 503, 710, 994, 1014
Hpy8I GTNNAC 4 cut(s) 422, 469, 865, 928
Hpy99I CGWCG 3 cut(s) 74, 209, 231
HpyCH4III ACNGT 1 cut(s) 649
HpyCH4IV ACGT 2 cut(s) 677, 861
HpyCH4V TGCA 6 cut(s) 171, 188, 652, 739, 745, 790
HpyF10VI GCNNNNNNNGC 7 cut(s) 71, 278, 302, 311, 376, 959, 973
HpyF3I CTNAG 1 cut(s) 957
HpySE526I ACGT 2 cut(s) 677, 861
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 2 cut(s) 118, 805
HspAI GCGC 3 cut(s) 155, 213, 370
KroI GCCGGC 1 cut(s) 310
KroNI GCCGGC 1 cut(s) 312
Ksp22I TGATCA 1 cut(s) 798
KspAI GTTAAC 2 cut(s) 865, 928
Kzo9I GATC 6 cut(s) 117, 182, 798, 805, 856, 913
LmnI GCTCC 5 cut(s) 286, 310, 319, 577, 610
Lsp1109I GCAGC 6 cut(s) 354, 412, 625, 802, 805, 940
LweI GCATC 1 cut(s) 158
MaeI CTAG 1 cut(s) 762
MaeII ACGT 2 cut(s) 677, 861
MaeIII GTNAC 1 cut(s) 682
MalI GATC 6 cut(s) 119, 184, 800, 807, 858, 915
MbiI CCGCTC 1 cut(s) 151
MboI GATC 6 cut(s) 117, 182, 798, 805, 856, 913
MboII GAAGA 9 cut(s) 37, 98, 211, 246, 454, 496, 617, 840, 982
MfeI CAATTG 1 cut(s) 462
MflI RGATCY 1 cut(s) 182
MhlI GDGCHC 2 cut(s) 381, 848
MlsI TGGCCA 1 cut(s) 594
MluCI AATT 9 cut(s) 125, 144, 462, 473, 491, 550, 653, 729, 747
MluNI TGGCCA 1 cut(s) 594
MlyI GAGTC 1 cut(s) 679
Mox20I TGGCCA 1 cut(s) 594
MroNI GCCGGC 1 cut(s) 310
MroXI GAANNNNTTC 1 cut(s) 524
MscI TGGCCA 1 cut(s) 594
MseI TTAA 5 cut(s) 549, 656, 864, 876, 927
MslI CAYNNNNRTG 1 cut(s) 680
Msp20I TGGCCA 1 cut(s) 594
MspA1I CMGCKG 2 cut(s) 367, 796
MspI CCGG 2 cut(s) 311, 638
MspR9I CCNGG 1 cut(s) 638
MunI CAATTG 1 cut(s) 462
MvnI CGCG 2 cut(s) 155, 850
MwoI GCNNNNNNNGC 7 cut(s) 71, 278, 302, 311, 376, 959, 973
NaeI GCCGGC 1 cut(s) 312
NciI CCSGG 1 cut(s) 638
NdeII GATC 6 cut(s) 117, 182, 798, 805, 856, 913
NgoMIV GCCGGC 1 cut(s) 310
NlaIII CATG 2 cut(s) 118, 805
NlaIV GGNNCC 7 cut(s) 282, 306, 315, 630, 642, 702, 846
NmeAIII GCCGAG 2 cut(s) 100, 265
NmuCI GTSAC 1 cut(s) 682
NspV TTCGAA 2 cut(s) 129, 482
OliI CACNNNNGTG 1 cut(s) 680
PdiI GCCGGC 1 cut(s) 312
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 3 cut(s) 100, 386, 881
PflMI CCANNNNNTGG 1 cut(s) 601
PleI GAGTC 1 cut(s) 679
PpsI GAGTC 1 cut(s) 679
PspFI CCCAGC 1 cut(s) 903
PspN4I GGNNCC 7 cut(s) 282, 306, 315, 630, 642, 702, 846
PspOMI GGGCCC 1 cut(s) 844
PspPI GGNCC 5 cut(s) 239, 628, 700, 844, 845
PstI CTGCAG 1 cut(s) 792
PstNI CAGNNNCTG 1 cut(s) 796
PsuI RGATCY 1 cut(s) 182
PvuII CAGCTG 1 cut(s) 796
RseI CAYNNNNRTG 1 cut(s) 680
SaqAI TTAA 5 cut(s) 549, 656, 864, 876, 927
Sau3AI GATC 6 cut(s) 117, 182, 798, 805, 856, 913
Sau96I GGNCC 5 cut(s) 239, 628, 700, 844, 845
SchI GAGTC 1 cut(s) 679
ScrFI CCNGG 1 cut(s) 638
SduI GDGCHC 2 cut(s) 381, 848
SetI ASST 8 cut(s) 87, 244, 510, 582, 680, 798, 864, 870
SfaNI GCATC 1 cut(s) 158
SfcI CTRYAG 2 cut(s) 788, 985
SfuI TTCGAA 2 cut(s) 129, 482
SinI GGWCC 2 cut(s) 239, 628
SmiMI CAYNNNNRTG 1 cut(s) 680
SmlI CTYRAG 3 cut(s) 412, 509, 693
SmoI CTYRAG 3 cut(s) 412, 509, 693
Sse9I AATT 9 cut(s) 125, 144, 462, 473, 491, 550, 653, 729, 747
SsiI CCGC 9 cut(s) 137, 149, 210, 296, 308, 365, 689, 698, 848
SspMI CTAG 1 cut(s) 762
StyD4I CCNGG 1 cut(s) 636
StyI CCWWGG 2 cut(s) 300, 427
TaaI ACNGT 1 cut(s) 649
TaiI ACGT 2 cut(s) 680, 864
TaqI TCGA 4 cut(s) 129, 375, 482, 978
TasI AATT 9 cut(s) 125, 144, 462, 473, 491, 550, 653, 729, 747
TauI GCSGC 4 cut(s) 213, 299, 367, 701
TfiI GAWTC 3 cut(s) 100, 386, 881
Tru1I TTAA 5 cut(s) 549, 656, 864, 876, 927
Tru9I TTAA 5 cut(s) 549, 656, 864, 876, 927
TscAI CASTG 2 cut(s) 264, 388
TseFI GTSAC 1 cut(s) 682
TseI GCWGC 6 cut(s) 367, 400, 613, 790, 793, 953
Tsp45I GTSAC 1 cut(s) 682
TspDTI ATGAA 1 cut(s) 92
TspGWI ACGGA 1 cut(s) 641
TspRI CASTG 2 cut(s) 264, 388
Van91I CCANNNNNTGG 1 cut(s) 601
VpaK11BI GGWCC 2 cut(s) 239, 628
XapI RAATTY 2 cut(s) 125, 747
XmnI GAANNNNTTC 1 cut(s) 524
XspI CTAG 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.