pycom15g01050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012583
Physical Location & Seq
Reverse (-)
565332 .. 566549
1218 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g01050.1

Sequence Viewer

Length: 837 bp
ATGGAACAGAGAGAAACACTAACAATGGAACAAAGGCTCCTCCTCTTCCCCTGTTTTCCGAACGCCAGCGACGGCGAGATAGAGGTGGCGAATATCCTGATTCATCTTCCTCACATGATCTCCGAATTGGAGACTCCGCTTCCAATTCCACTCGAGCTCATCTGGGGATGCAAGGGAAAGAGATCTGTACTCGCCCTGAAGAACGACGGATGCGCTTCCACTCTGCGTCGTCTGATTGGACCTAATTTTTTGTGTTCACTGCAACTCTGGGCTTCTCTGGCTCGCCGGCCTTCGGCGGCCAAGGCTCCGCCGCCGCCCCCGGTCCCTAATGCAGTCAAGGAGGAGACTTCCAGCCCTGATACGCCGCTGTGCTTCGAGCCCAGTGAATCTGACGAGAAGCAGCCTGACCACTTGAGTAGTAAACTCTTTAGTCAAAAAAGGAAGAGAGAGGACTGGATTGCAATTGTGGACGAATTGACTTCGAAGGAAAAATGGCGACATCAAGAACAGGAGACCCATGTGGATACGGATCCAAGTTCGAAACCAGCCGCGCAATTAAGCCAACTGACTCAACTCACATATGTAACTTCGGCTCCTGCGGCCCCAAAACAAGAGCCTTACCAACTTCCTCATCAAGTGCAAATGCCAATAATGCAAATTCATCCCCCCAGTCTGATTCTTTATCAACGTCAACTGCAGCAGCTGATCATGGATCAGACGGCTAAGACTCAAAGAAGAGAGATCAATGCGGGCCCACACGGTGGTCACATTAACCTTTGTTTTAGGGATGCAAACGTGTTGAACTCCTCCCAGCCGTTTGATCGTTCGCTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

279

Amino Acids

31.29

Weight (kDa)

6.31

Isoelectric Point (pI)

65.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 761
AccII CGCG 1 cut(s) 551
AciI CCGC 9 cut(s) 137, 296, 308, 311, 314, 365, 549, 599, 749
AclWI GGATC 3 cut(s) 524, 537, 720
AcoI YGGCCR 1 cut(s) 297
AcsI RAATTY 1 cut(s) 657
AcuI CTGAAG 1 cut(s) 218
AdeI CACNNNGTG 1 cut(s) 761
AfaI GTAC 1 cut(s) 189
AfiI CCNNNNNNNGG 2 cut(s) 292, 761
AflIII ACRYGT 1 cut(s) 795
AgsI TTSAA 1 cut(s) 802
AloI GAACNNNNNNTCC 2 cut(s) 21, 53
AluBI AGCT 2 cut(s) 157, 703
AluI AGCT 2 cut(s) 157, 703
Alw21I GWGCWC 1 cut(s) 159
Alw26I GTCTC 3 cut(s) 125, 338, 506
AlwI GGATC 3 cut(s) 524, 537, 720
AlwNI CAGNNNCTG 1 cut(s) 703
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 4 cut(s) 287, 297, 600, 751
ApaI GGGCCC 1 cut(s) 755
ApeKI GCWGC 3 cut(s) 400, 697, 700
ApoI RAATTY 1 cut(s) 657
AspLEI GCGC 2 cut(s) 215, 553
AspS9I GGNCC 5 cut(s) 239, 322, 601, 751, 752
AsuC2I CCSGG 1 cut(s) 320
AsuII TTCGAA 2 cut(s) 482, 539
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 2 cut(s) 239, 322
BaeGI GKGCMC 1 cut(s) 755
BamHI GGATCC 1 cut(s) 529
BanII GRGCYC 3 cut(s) 159, 381, 755
Bbv12I GWGCWC 1 cut(s) 159
BbvI GCAGC 3 cut(s) 412, 709, 712
BceAI ACGGC 3 cut(s) 88, 735, 799
BciVI GTATCC 1 cut(s) 517
BclI TGATCA 1 cut(s) 705
BcnI CCSGG 1 cut(s) 320
BcoDI GTCTC 3 cut(s) 125, 338, 506
BfmI CTRYAG 1 cut(s) 695
BfuI GTATCC 1 cut(s) 517
BglII AGATCT 1 cut(s) 182
BisI GCNGC 9 cut(s) 297, 311, 314, 365, 401, 549, 600, 698, 701
BlsI GCNGC 9 cut(s) 298, 312, 315, 366, 402, 550, 601, 699, 702
Bme1390I CCNGG 1 cut(s) 320
Bme18I GGWCC 2 cut(s) 239, 322
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 5 cut(s) 239, 322, 601, 751, 752
BmiI GGNNCC 7 cut(s) 38, 306, 324, 531, 594, 603, 753
BmrFI CCNGG 1 cut(s) 320
BmrI ACTGGG 2 cut(s) 375, 663
BmsI GCATC 3 cut(s) 158, 200, 778
BmuI ACTGGG 2 cut(s) 375, 663
Bpu14I TTCGAA 2 cut(s) 482, 539
BpuEI CTTGAG 1 cut(s) 433
BpuMI CCSGG 1 cut(s) 320
BsaBI GATNNNNATC 1 cut(s) 528
BsaI GGTCTC 1 cut(s) 506
BsaJI CCNNGG 2 cut(s) 300, 318
BsaXI ACNNNNNCTCC 6 cut(s) 21, 51, 104, 134, 577, 607
Bsc4I CCNNNNNNNGG 2 cut(s) 292, 761
Bse118I RCCGGY 1 cut(s) 285
Bse1I ACTGG 3 cut(s) 381, 458, 669
Bse8I GATNNNNATC 1 cut(s) 528
BseDI CCNNGG 2 cut(s) 300, 318
BseGI GGATG 4 cut(s) 173, 215, 661, 793
BseJI GATNNNNATC 1 cut(s) 528
BseLI CCNNNNNNNGG 2 cut(s) 292, 761
BseNI ACTGG 3 cut(s) 381, 458, 669
BseRI GAGGAG 4 cut(s) 29, 32, 356, 796
BseSI GKGCMC 1 cut(s) 755
BseXI GCAGC 3 cut(s) 412, 709, 712
BseYI CCCAGC 1 cut(s) 810
Bsh1236I CGCG 1 cut(s) 551
BshFI GGCC 4 cut(s) 289, 299, 602, 753
BsiHKAI GWGCWC 1 cut(s) 159
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 2 cut(s) 286, 320
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 2 cut(s) 292, 761
BsmAI GTCTC 3 cut(s) 125, 338, 506
BsmFI GGGAC 1 cut(s) 308
BsnI GGCC 4 cut(s) 289, 299, 602, 753
Bso31I GGTCTC 1 cut(s) 506
BsoBI CYCGRG 1 cut(s) 152
Bsp119I TTCGAA 2 cut(s) 482, 539
Bsp120I GGGCCC 1 cut(s) 751
Bsp1286I GDGCHC 3 cut(s) 159, 381, 755
Bsp143I GATC 7 cut(s) 117, 182, 529, 705, 712, 741, 820
BspACI CCGC 9 cut(s) 137, 296, 308, 311, 314, 365, 549, 599, 749
BspANI GGCC 4 cut(s) 289, 299, 602, 753
BspFNI CGCG 1 cut(s) 551
BspLI GGNNCC 7 cut(s) 38, 306, 324, 531, 594, 603, 753
BspMAI CTGCAG 1 cut(s) 699
BspPI GGATC 3 cut(s) 524, 537, 720
BspT104I TTCGAA 2 cut(s) 482, 539
BspTNI GGTCTC 1 cut(s) 506
BsrFI RCCGGY 1 cut(s) 285
BsrI ACTGG 3 cut(s) 381, 458, 669
BssAI RCCGGY 1 cut(s) 285
BssECI CCNNGG 2 cut(s) 300, 318
BssMI GATC 7 cut(s) 117, 182, 529, 705, 712, 741, 820
BssT1I CCWWGG 1 cut(s) 300
Bst4CI ACNGT 1 cut(s) 761
Bst6I CTCTTC 3 cut(s) 50, 437, 730
BstBI TTCGAA 2 cut(s) 482, 539
BstC8I GCNNGC 4 cut(s) 67, 283, 287, 751
BstDEI CTNAG 1 cut(s) 723
BstF5I GGATG 4 cut(s) 173, 215, 661, 793
BstFNI CGCG 1 cut(s) 551
BstHHI GCGC 2 cut(s) 215, 553
BstKTI GATC 7 cut(s) 120, 185, 532, 708, 715, 744, 823
BstMAI GTCTC 3 cut(s) 125, 338, 506
BstMBI GATC 7 cut(s) 117, 182, 529, 705, 712, 741, 820
BstMWI GCNNNNNNNGC 4 cut(s) 278, 302, 599, 652
BstSCI CCNGG 1 cut(s) 318
BstSFI CTRYAG 1 cut(s) 695
BstSLI GKGCMC 1 cut(s) 755
BstUI CGCG 1 cut(s) 551
BstV1I GCAGC 3 cut(s) 412, 709, 712
BstX2I RGATCY 2 cut(s) 182, 529
BstYI RGATCY 2 cut(s) 182, 529
BsuI GTATCC 1 cut(s) 517
BsuRI GGCC 4 cut(s) 289, 299, 602, 753
BtsCI GGATG 4 cut(s) 173, 215, 661, 793
BtsI GCAGTG 1 cut(s) 257
BtsIMutI CAGTG 2 cut(s) 257, 388
Cac8I GCNNGC 4 cut(s) 67, 283, 287, 751
CaiI CAGNNNCTG 1 cut(s) 703
CfoI GCGC 2 cut(s) 215, 553
Cfr10I RCCGGY 1 cut(s) 285
Cfr13I GGNCC 5 cut(s) 239, 322, 601, 751, 752
CseI GACGC 1 cut(s) 215
Csp6I GTAC 1 cut(s) 188
CviAII CATG 3 cut(s) 115, 518, 709
CviQI GTAC 1 cut(s) 188
DdeI CTNAG 1 cut(s) 723
DpnI GATC 7 cut(s) 119, 184, 531, 707, 714, 743, 822
DpnII GATC 7 cut(s) 117, 182, 529, 705, 712, 741, 820
DraIII CACNNNGTG 1 cut(s) 761
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 3 cut(s) 50, 437, 730
EarI CTCTTC 3 cut(s) 50, 437, 730
EciI GGCGGA 1 cut(s) 297
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 3 cut(s) 159, 381, 755
Eco31I GGTCTC 1 cut(s) 506
Eco47I GGWCC 2 cut(s) 239, 322
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 218
Eco88I CYCGRG 1 cut(s) 152
EcoICRI GAGCTC 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 3 cut(s) 159, 381, 755
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 3 cut(s) 118, 521, 712
FaiI YATR 5 cut(s) 116, 519, 580, 582, 710
FaqI GGGAC 1 cut(s) 308
FatI CATG 3 cut(s) 114, 517, 708
FauI CCCGC 1 cut(s) 742
FauNDI CATATG 1 cut(s) 580
FbaI TGATCA 1 cut(s) 705
Fnu4HI GCNGC 9 cut(s) 297, 311, 314, 365, 401, 549, 600, 698, 701
FokI GGATG 4 cut(s) 180, 222, 648, 800
FriOI GRGCYC 3 cut(s) 159, 381, 755
Fsp4HI GCNGC 9 cut(s) 297, 311, 314, 365, 401, 549, 600, 698, 701
GlaI GCGC 2 cut(s) 214, 552
GluI GCNGC 9 cut(s) 297, 311, 314, 365, 401, 549, 600, 698, 701
GsaI CCCAGC 1 cut(s) 814
HaeIII GGCC 4 cut(s) 289, 299, 602, 753
HapII CCGG 2 cut(s) 286, 320
HgaI GACGC 1 cut(s) 215
HhaI GCGC 2 cut(s) 215, 553
Hin1II CATG 3 cut(s) 118, 521, 712
Hin6I GCGC 2 cut(s) 213, 551
HinP1I GCGC 2 cut(s) 213, 551
HincII GTYRAC 1 cut(s) 692
HindII GTYRAC 1 cut(s) 692
HinfI GANTC 6 cut(s) 100, 133, 386, 568, 676, 727
HpaII CCGG 2 cut(s) 286, 320
Hpy166II GTNNAC 4 cut(s) 257, 422, 469, 692
Hpy188I TCNGA 6 cut(s) 60, 124, 234, 391, 675, 717
Hpy188III TCNNGA 2 cut(s) 97, 503
Hpy8I GTNNAC 4 cut(s) 257, 422, 469, 692
Hpy99I CGWCG 3 cut(s) 74, 209, 231
HpyAV CCTTC 2 cut(s) 300, 478
HpyCH4III ACNGT 1 cut(s) 761
HpyCH4IV ACGT 2 cut(s) 688, 795
HpyCH4V TGCA 8 cut(s) 171, 262, 332, 461, 640, 655, 697, 791
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 302, 599, 652
HpyF3I CTNAG 1 cut(s) 723
HpySE526I ACGT 2 cut(s) 688, 795
Hsp92II CATG 3 cut(s) 118, 521, 712
HspAI GCGC 2 cut(s) 213, 551
KroI GCCGGC 1 cut(s) 285
KroNI GCCGGC 1 cut(s) 287
Ksp22I TGATCA 1 cut(s) 705
Kzo9I GATC 7 cut(s) 117, 182, 529, 705, 712, 741, 820
LmnI GCTCC 3 cut(s) 42, 310, 598
Lsp1109I GCAGC 3 cut(s) 412, 709, 712
LweI GCATC 3 cut(s) 158, 200, 778
MaeII ACGT 2 cut(s) 688, 795
MaeIII GTNAC 2 cut(s) 583, 764
MalI GATC 7 cut(s) 119, 184, 531, 707, 714, 743, 822
MboI GATC 7 cut(s) 117, 182, 529, 705, 712, 741, 820
MboII GAAGA 5 cut(s) 37, 98, 211, 454, 747
MfeI CAATTG 1 cut(s) 462
MflI RGATCY 2 cut(s) 182, 529
MhlI GDGCHC 3 cut(s) 159, 381, 755
MluCI AATT 7 cut(s) 125, 144, 244, 462, 473, 554, 657
MlyI GAGTC 3 cut(s) 127, 562, 721
MnlI CCTC 8 cut(s) 50, 53, 76, 120, 334, 442, 639, 817
MroNI GCCGGC 1 cut(s) 285
MseI TTAA 3 cut(s) 557, 771, 835
MspA1I CMGCKG 2 cut(s) 367, 703
MspI CCGG 2 cut(s) 286, 320
MspR9I CCNGG 1 cut(s) 320
MunI CAATTG 1 cut(s) 462
MvnI CGCG 1 cut(s) 551
MwoI GCNNNNNNNGC 4 cut(s) 278, 302, 599, 652
NaeI GCCGGC 1 cut(s) 287
NciI CCSGG 1 cut(s) 320
NdeI CATATG 1 cut(s) 580
NdeII GATC 7 cut(s) 117, 182, 529, 705, 712, 741, 820
NgoMIV GCCGGC 1 cut(s) 285
NlaIII CATG 3 cut(s) 118, 521, 712
NlaIV GGNNCC 7 cut(s) 38, 306, 324, 531, 594, 603, 753
NmuCI GTSAC 1 cut(s) 764
NspV TTCGAA 2 cut(s) 482, 539
PaeR7I CTCGAG 1 cut(s) 152
PdiI GCCGGC 1 cut(s) 287
PfeI GAWTC 3 cut(s) 100, 386, 676
PflMI CCANNNNNTGG 1 cut(s) 761
PkrI GCNGC 9 cut(s) 298, 312, 315, 366, 402, 550, 601, 699, 702
PleI GAGTC 3 cut(s) 127, 562, 721
PpsI GAGTC 3 cut(s) 127, 562, 721
Psp124BI GAGCTC 1 cut(s) 159
PspFI CCCAGC 1 cut(s) 810
PspN4I GGNNCC 7 cut(s) 38, 306, 324, 531, 594, 603, 753
PspOMI GGGCCC 1 cut(s) 751
PspPI GGNCC 5 cut(s) 239, 322, 601, 751, 752
PspXI VCTCGAGB 1 cut(s) 152
PstI CTGCAG 1 cut(s) 699
PstNI CAGNNNCTG 1 cut(s) 703
PsuI RGATCY 2 cut(s) 182, 529
PvuII CAGCTG 1 cut(s) 703
RsaI GTAC 1 cut(s) 189
RsaNI GTAC 1 cut(s) 188
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 3 cut(s) 557, 771, 835
SatI GCNGC 9 cut(s) 297, 311, 314, 365, 401, 549, 600, 698, 701
Sau3AI GATC 7 cut(s) 117, 182, 529, 705, 712, 741, 820
Sau96I GGNCC 5 cut(s) 239, 322, 601, 751, 752
SchI GAGTC 3 cut(s) 127, 562, 721
ScrFI CCNGG 1 cut(s) 320
SduI GDGCHC 3 cut(s) 159, 381, 755
SetI ASST 7 cut(s) 87, 159, 244, 691, 705, 777, 798
SfaNI GCATC 3 cut(s) 158, 200, 778
SfcI CTRYAG 1 cut(s) 695
Sfr274I CTCGAG 1 cut(s) 152
SfuI TTCGAA 2 cut(s) 482, 539
SinI GGWCC 2 cut(s) 239, 322
SlaI CTCGAG 1 cut(s) 152
SmlI CTYRAG 2 cut(s) 152, 412
SmoI CTYRAG 2 cut(s) 152, 412
Sse9I AATT 7 cut(s) 125, 144, 244, 462, 473, 554, 657
SsiI CCGC 9 cut(s) 137, 296, 308, 311, 314, 365, 549, 599, 749
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 318
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 761
TaiI ACGT 2 cut(s) 691, 798
TaqI TCGA 4 cut(s) 153, 375, 482, 539
TasI AATT 7 cut(s) 125, 144, 244, 462, 473, 554, 657
TatI WGTACW 1 cut(s) 187
TauI GCSGC 6 cut(s) 299, 313, 316, 367, 551, 602
TfiI GAWTC 3 cut(s) 100, 386, 676
Tru1I TTAA 3 cut(s) 557, 771, 835
Tru9I TTAA 3 cut(s) 557, 771, 835
TscAI CASTG 2 cut(s) 264, 388
TseFI GTSAC 1 cut(s) 764
TseI GCWGC 3 cut(s) 400, 697, 700
Tsp45I GTSAC 1 cut(s) 764
TspDTI ATGAA 2 cut(s) 92, 650
TspGWI ACGGA 2 cut(s) 222, 542
TspRI CASTG 2 cut(s) 264, 388
Van91I CCANNNNNTGG 1 cut(s) 761
VpaK11BI GGWCC 2 cut(s) 239, 322
XapI RAATTY 1 cut(s) 657
XhoI CTCGAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.