Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0007648

Physical Location & Seq

Genomic Coordinates Reverse (-)

2300218 .. 2303033

Gene Structure 2816 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1032100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 885 bp

ATGTTGCGCCTCTGGAGCGGTTCTCATCAGCAAGTGAACGATCCGTTGAAGCACGACGATAAGATCAATGAGATGAAGGCTGCGATCGGACCGCTATCCGGACGCAGTTTGCAGTTCTGCACTGATGCATGCTTCAGGAGGTACTTGGAGGCGCGGAATTGGAACGTTGACAAAGCGAAGAAAATGTTGGATGAAACGCTCAAATGGAGGTCAACCTATAAGCCCGAGGAAATCCGCTGGCCCGAAGTTGCAAGTGAAGGCGAGACTGGAAAAGTTTACAGAGCAAGTTTTCGCGATCGTAGTGGGAGGAGTGTTCTTATACTGAGACCGGGAATGCAGAACACGGTGTCAATCGACAATCAAATGCGTCATCTGGTGTATCTGATTGAGAATGCTGTATTCAACCTTCCCGAGGGCCAAGAACAAATGGCGTGGCTGATTGACTTCACGGGGTGGACGCTAAGTACTACCGTGCCTGTGAAATCAGCTCGAGACACCATCAATATTTTACAGAACCACTACCCCGAGAGACTTGCAGTAGCATTTCTCTACAATCCCCCACGCATCTTCGAAGCGTTTTGGAAGATTGTCAAGTATTTCTTGGACAACAAGACATTCCACAAGGTTAAGTTCGTTTACCCGAAGAACAAAGATAGCGTGGAGCTTATGAAACAATATTTCGACCAGGAAAATCTCCCTACCGAGTTTGGAGGACAGGCCATACTAAACTATGACCACGAGGAATTCTCGAAATTAATGAATCAAGATGATGTGAAATCAGCTGCATTTTGGGGTCGCGATGAAAAGCTACCACATTGTGTTAATGGGCGTTCTGGAGCCGAGGTGGCTCCAGAACCGGTGAGCGTTACACCGGCAGCCAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRAL_TRIO CRAL_TRIO_N

Protein Analysis

295

Amino Acids

34.03

Weight (kDa)

7.71

Isoelectric Point (pI)

49.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRAL_TRIO_N	PF03765	37 - 63	4.9e-07	CRAL/TRIO, N-terminal domain
CRAL_TRIO	PF00650	89 - 238	6.2e-37	CRAL/TRIO domain
CRAL_TRIO_2	PF13716	120 - 247	1e-09	Divergent CRAL/TRIO domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007648)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75170 AT1G75170 AT1G75170
fragaria_vesca	FvH4_2g39850 FvH4_2g39850
malus_domestica	MD08G1032100.v1.1 MD15G1028100.v1.1
prunus_persica	Prupe.1G379800_v2.0.a1 Prupe.1G379800_v2.0.a1 Prupe.1G379800_v2.0.a1
pyrus_communis	pycom08g02540 pycom15g02450
rosa_chinensis	RchiOBHm_Chr6g0300991
rosa_laevigata	RLG00000011287 RLG00000034667
rosa_multiflora	Rmu_co8375471.1_g000001 Rmu_sc0011701.1_g000029
rosa_roxburghii	Rroxscaffold_7G00167280
rosa_rugosa	Rorug06G0299800 Rorug06G0299800
rosa_samantha	Rh6AG413700 Rh6BG420600 Rh6CG428200 Rh6DG413600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	18
AccII	CGCG	3 cut(s)	154, 294, 798
AccIII	TCCGGA	1 cut(s)	98
AciI	CCGC	4 cut(s)	18, 92, 154, 235
AclI	AACGTT	1 cut(s)	165
AclWI	GGATC	1 cut(s)	35
AcsI	RAATTY	1 cut(s)	743
AcuI	CTGAAG	1 cut(s)	118
AdeI	CACNNNGTG	2 cut(s)	453, 818
AfaI	GTAC	2 cut(s)	143, 466
AfiI	CCNNNNNNNGG	2 cut(s)	98, 412
AgeI	ACCGGT	1 cut(s)	856
AgsI	TTSAA	2 cut(s)	49, 403
AjnI	CCWGG	1 cut(s)	684
AjuI	GAANNNNNNNTTGG	2 cut(s)	170, 202
AluBI	AGCT	5 cut(s)	488, 664, 782, 808, 882
AluI	AGCT	5 cut(s)	488, 664, 782, 808, 882
Alw26I	GTCTC	4 cut(s)	257, 319, 486, 523
AlwI	GGATC	1 cut(s)	35
Ama87I	CYCGRG	4 cut(s)	224, 410, 489, 524
Aor13HI	TCCGGA	1 cut(s)	98
AoxI	GGCC	3 cut(s)	239, 415, 717
ApeKI	GCWGC	3 cut(s)	80, 782, 875
ApoI	RAATTY	1 cut(s)	743
AseI	ATTAAT	1 cut(s)	755
AsiGI	ACCGGT	1 cut(s)	856
AspLEI	GCGC	2 cut(s)	9, 154
AspS9I	GGNCC	3 cut(s)	89, 240, 415
AsuC2I	CCSGG	1 cut(s)	330
AsuHPI	GGTGA	1 cut(s)	871
AsuII	TTCGAA	1 cut(s)	570
AvaI	CYCGRG	4 cut(s)	224, 410, 489, 524
AvaII	GGWCC	1 cut(s)	89
BarI	GAAGNNNNNNTAC	2 cut(s)	390, 422
BauI	CACGAG	1 cut(s)	737
BbvI	GCAGC	2 cut(s)	67, 769
BccI	CCATC	1 cut(s)	506
BcgI	CGANNNNNNTGC	2 cut(s)	515, 549
BciT130I	CCWGG	1 cut(s)	686
BcnI	CCSGG	1 cut(s)	330
BcoDI	GTCTC	4 cut(s)	257, 319, 486, 523
BglI	GCCNNNNNGGC	1 cut(s)	845
BisI	GCNGC	3 cut(s)	81, 783, 876
BlsI	GCNGC	3 cut(s)	82, 784, 877
BmcAI	AGTACT	1 cut(s)	466
Bme1390I	CCNGG	2 cut(s)	330, 686
Bme18I	GGWCC	1 cut(s)	89
BmeT110I	CYCGRG	4 cut(s)	224, 410, 489, 524
BmgT120I	GGNCC	3 cut(s)	89, 240, 415
BmiI	GGNNCC	2 cut(s)	838, 849
BmrFI	CCNGG	2 cut(s)	330, 686
BmsI	GCATC	2 cut(s)	115, 573
BplI	GAGNNNNNCTC	4 cut(s)	7, 39, 731, 763
BpmI	CTGGAG	3 cut(s)	34, 834, 855
Bpu14I	TTCGAA	1 cut(s)	570
BpuMI	CCSGG	1 cut(s)	330
BsaI	GGTCTC	1 cut(s)	319
BsaJI	CCNNGG	3 cut(s)	225, 411, 840
BsaWI	WCCGGW	2 cut(s)	98, 856
BsaXI	ACNNNNNCTCC	2 cut(s)	828, 858
Bsc4I	CCNNNNNNNGG	2 cut(s)	98, 412
Bse118I	RCCGGY	2 cut(s)	856, 871
Bse1I	ACTGG	1 cut(s)	271
BseAI	TCCGGA	1 cut(s)	98
BseBI	CCWGG	1 cut(s)	686
BseDI	CCNNGG	3 cut(s)	225, 411, 840
BseGI	GGATG	1 cut(s)	196
BseLI	CCNNNNNNNGG	2 cut(s)	98, 412
BseMII	CTCAG	1 cut(s)	314
BseNI	ACTGG	1 cut(s)	271
BseRI	GAGGAG	1 cut(s)	322
BseXI	GCAGC	2 cut(s)	67, 769
BsgI	GTGCAG	1 cut(s)	103
Bsh1236I	CGCG	3 cut(s)	154, 294, 798
Bsh1285I	CGRYCG	2 cut(s)	87, 298
BshFI	GGCC	3 cut(s)	241, 417, 719
BshTI	ACCGGT	1 cut(s)	856
BsiEI	CGRYCG	2 cut(s)	87, 298
BsiHKCI	CYCGRG	4 cut(s)	224, 410, 489, 524
BsiSI	CCGG	4 cut(s)	99, 329, 857, 872
BslI	CCNNNNNNNGG	2 cut(s)	98, 412
BsmAI	GTCTC	4 cut(s)	257, 319, 486, 523
BsmI	GAATGC	2 cut(s)	339, 397
BsnI	GGCC	3 cut(s)	241, 417, 719
Bso31I	GGTCTC	1 cut(s)	319
BsoBI	CYCGRG	4 cut(s)	224, 410, 489, 524
Bsp119I	TTCGAA	1 cut(s)	570
Bsp13I	TCCGGA	1 cut(s)	98
Bsp143I	GATC	4 cut(s)	40, 63, 84, 295
Bsp68I	TCGCGA	2 cut(s)	294, 798
BspACI	CCGC	4 cut(s)	18, 92, 154, 235
BspANI	GGCC	3 cut(s)	241, 417, 719
BspCNI	CTCAG	1 cut(s)	315
BspEI	TCCGGA	1 cut(s)	98
BspFNI	CGCG	3 cut(s)	154, 294, 798
BspLI	GGNNCC	2 cut(s)	838, 849
BspPI	GGATC	1 cut(s)	35
BspT104I	TTCGAA	1 cut(s)	570
BspTNI	GGTCTC	1 cut(s)	319
BsrBI	CCGCTC	1 cut(s)	18
BsrFI	RCCGGY	2 cut(s)	856, 871
BsrI	ACTGG	1 cut(s)	271
BssAI	RCCGGY	2 cut(s)	856, 871
BssECI	CCNNGG	3 cut(s)	225, 411, 840
BssMI	GATC	4 cut(s)	40, 63, 84, 295
BssSI	CACGAG	1 cut(s)	737
Bst2BI	CACGAG	1 cut(s)	737
Bst2UI	CCWGG	1 cut(s)	686
Bst4CI	ACNGT	2 cut(s)	346, 472
BstBI	TTCGAA	1 cut(s)	570
BstC8I	GCNNGC	3 cut(s)	130, 239, 880
BstDEI	CTNAG	2 cut(s)	323, 461
BstENI	CCTNNNNNAGG	1 cut(s)	410
BstF5I	GGATG	1 cut(s)	196
BstFNI	CGCG	3 cut(s)	154, 294, 798
BstHHI	GCGC	2 cut(s)	9, 154
BstKTI	GATC	4 cut(s)	43, 66, 87, 298
BstMAI	GTCTC	4 cut(s)	257, 319, 486, 523
BstMBI	GATC	4 cut(s)	40, 63, 84, 295
BstMCI	CGRYCG	2 cut(s)	87, 298
BstMWI	GCNNNNNNNGC	2 cut(s)	15, 845
BstNI	CCWGG	1 cut(s)	686
BstNSI	RCATGY	1 cut(s)	132
BstSCI	CCNGG	2 cut(s)	328, 684
BstUI	CGCG	3 cut(s)	154, 294, 798
BstV1I	GCAGC	2 cut(s)	67, 769
BsuRI	GGCC	3 cut(s)	241, 417, 719
BtgZI	GCGATG	1 cut(s)	813
BtsCI	GGATG	1 cut(s)	196
BtsIMutI	CAGTG	1 cut(s)	120
BtuMI	TCGCGA	2 cut(s)	294, 798
Cac8I	GCNNGC	3 cut(s)	130, 239, 880
CfoI	GCGC	2 cut(s)	9, 154
Cfr10I	RCCGGY	2 cut(s)	856, 871
Cfr13I	GGNCC	3 cut(s)	89, 240, 415
CpoI	CGGWCCG	1 cut(s)	89
CseI	GACGC	3 cut(s)	111, 356, 466
Csp6I	GTAC	2 cut(s)	142, 465
CspAI	ACCGGT	1 cut(s)	856
CspCI	CAANNNNNGTGG	2 cut(s)	413, 448
CspI	CGGWCCG	1 cut(s)	89
CviAII	CATG	1 cut(s)	129
CviQI	GTAC	2 cut(s)	142, 465
DdeI	CTNAG	2 cut(s)	323, 461
DpnI	GATC	4 cut(s)	42, 65, 86, 297
DpnII	GATC	4 cut(s)	40, 63, 84, 295
DraIII	CACNNNGTG	2 cut(s)	453, 818
Eco31I	GGTCTC	1 cut(s)	319
Eco47I	GGWCC	1 cut(s)	89
Eco57I	CTGAAG	1 cut(s)	118
Eco88I	CYCGRG	4 cut(s)	224, 410, 489, 524
EcoNI	CCTNNNNNAGG	1 cut(s)	410
EcoRI	GAATTC	1 cut(s)	743
EcoRII	CCWGG	1 cut(s)	684
EcoT22I	ATGCAT	1 cut(s)	130
FaeI	CATG	1 cut(s)	132
FaiI	YATR	6 cut(s)	130, 219, 320, 668, 722, 732
FalI	AAGNNNNNCTT	2 cut(s)	584, 616
FatI	CATG	1 cut(s)	128
Fnu4HI	GCNGC	3 cut(s)	81, 783, 876
FokI	GGATG	1 cut(s)	203
Fsp4HI	GCNGC	3 cut(s)	81, 783, 876
GlaI	GCGC	2 cut(s)	8, 153
GluI	GCNGC	3 cut(s)	81, 783, 876
GsuI	CTGGAG	3 cut(s)	34, 834, 855
HaeIII	GGCC	3 cut(s)	241, 417, 719
HapII	CCGG	4 cut(s)	99, 329, 857, 872
HgaI	GACGC	3 cut(s)	111, 356, 466
HhaI	GCGC	2 cut(s)	9, 154
Hin1II	CATG	1 cut(s)	132
Hin6I	GCGC	2 cut(s)	7, 152
HinP1I	GCGC	2 cut(s)	7, 152
HincII	GTYRAC	2 cut(s)	169, 213
HindII	GTYRAC	2 cut(s)	169, 213
HinfI	GANTC	1 cut(s)	760
HpaII	CCGG	4 cut(s)	99, 329, 857, 872
HphI	GGTGA	1 cut(s)	871
Hpy166II	GTNNAC	6 cut(s)	37, 169, 213, 277, 456, 637
Hpy188I	TCNGA	2 cut(s)	89, 384
Hpy8I	GTNNAC	6 cut(s)	37, 169, 213, 277, 456, 637
Hpy99I	CGWCG	1 cut(s)	59
HpyAV	CCTTC	3 cut(s)	70, 251, 416
HpyCH4III	ACNGT	2 cut(s)	346, 472
HpyCH4IV	ACGT	1 cut(s)	165
HpyCH4V	TGCA	7 cut(s)	112, 120, 128, 251, 337, 536, 785
HpyF10VI	GCNNNNNNNGC	2 cut(s)	15, 845
HpyF3I	CTNAG	2 cut(s)	323, 461
HpySE526I	ACGT	1 cut(s)	165
Hsp92II	CATG	1 cut(s)	132
HspAI	GCGC	2 cut(s)	7, 152
Kpn2I	TCCGGA	1 cut(s)	98
Kzo9I	GATC	4 cut(s)	40, 63, 84, 295
LmnI	GCTCC	4 cut(s)	15, 661, 836, 853
Lsp1109I	GCAGC	2 cut(s)	67, 769
LweI	GCATC	2 cut(s)	115, 573
MaeII	ACGT	1 cut(s)	165
MaeIII	GTNAC	1 cut(s)	865
MalI	GATC	4 cut(s)	42, 65, 86, 297
MbiI	CCGCTC	1 cut(s)	18
MboI	GATC	4 cut(s)	40, 63, 84, 295
MboII	GAAGA	4 cut(s)	190, 559, 595, 655
MluCI	AATT	3 cut(s)	157, 743, 752
MmeI	TCCRAC	1 cut(s)	168
Mph1103I	ATGCAT	1 cut(s)	130
MroI	TCCGGA	1 cut(s)	98
MseI	TTAA	3 cut(s)	627, 755, 822
MspA1I	CMGCKG	3 cut(s)	237, 782, 882
MspI	CCGG	4 cut(s)	99, 329, 857, 872
MspR9I	CCNGG	2 cut(s)	330, 686
Mva1269I	GAATGC	2 cut(s)	339, 397
MvaI	CCWGG	1 cut(s)	686
MvnI	CGCG	3 cut(s)	154, 294, 798
MwoI	GCNNNNNNNGC	2 cut(s)	15, 845
NciI	CCSGG	1 cut(s)	330
NdeII	GATC	4 cut(s)	40, 63, 84, 295
NlaIII	CATG	1 cut(s)	132
NlaIV	GGNNCC	2 cut(s)	838, 849
NmeAIII	GCCGAG	1 cut(s)	865
NruI	TCGCGA	2 cut(s)	294, 798
NsiI	ATGCAT	1 cut(s)	130
NspI	RCATGY	1 cut(s)	132
NspV	TTCGAA	1 cut(s)	570
PaeI	GCATGC	1 cut(s)	132
PaeR7I	CTCGAG	1 cut(s)	489
PctI	GAATGC	2 cut(s)	339, 397
PfeI	GAWTC	1 cut(s)	760
PinAI	ACCGGT	1 cut(s)	856
PkrI	GCNGC	3 cut(s)	82, 784, 877
Ple19I	CGATCG	2 cut(s)	87, 298
PshBI	ATTAAT	1 cut(s)	755
Psp1406I	AACGTT	1 cut(s)	165
Psp6I	CCWGG	1 cut(s)	684
PspGI	CCWGG	1 cut(s)	684
PspN4I	GGNNCC	2 cut(s)	838, 849
PspPI	GGNCC	3 cut(s)	89, 240, 415
PvuI	CGATCG	2 cut(s)	87, 298
PvuII	CAGCTG	2 cut(s)	782, 882
RruI	TCGCGA	2 cut(s)	294, 798
RsaI	GTAC	2 cut(s)	143, 466
RsaNI	GTAC	2 cut(s)	142, 465
Rsr2I	CGGWCCG	1 cut(s)	89
RsrII	CGGWCCG	1 cut(s)	89
SaqAI	TTAA	3 cut(s)	627, 755, 822
SatI	GCNGC	3 cut(s)	81, 783, 876
Sau3AI	GATC	4 cut(s)	40, 63, 84, 295
Sau96I	GGNCC	3 cut(s)	89, 240, 415
ScaI	AGTACT	1 cut(s)	466
ScrFI	CCNGG	2 cut(s)	330, 686
SfaNI	GCATC	2 cut(s)	115, 573
Sfr274I	CTCGAG	1 cut(s)	489
SfuI	TTCGAA	1 cut(s)	570
SinI	GGWCC	1 cut(s)	89
SlaI	CTCGAG	1 cut(s)	489
SmlI	CTYRAG	1 cut(s)	489
SmoI	CTYRAG	1 cut(s)	489
SphI	GCATGC	1 cut(s)	132
Sse9I	AATT	3 cut(s)	157, 743, 752
SsiI	CCGC	4 cut(s)	18, 92, 154, 235
SspI	AATATT	2 cut(s)	505, 677
StyD4I	CCNGG	2 cut(s)	328, 684
TaaI	ACNGT	2 cut(s)	346, 472
TaiI	ACGT	1 cut(s)	168
TaqI	TCGA	5 cut(s)	354, 490, 570, 681, 749
TasI	AATT	3 cut(s)	157, 743, 752
TatI	WGTACW	1 cut(s)	464
TfiI	GAWTC	1 cut(s)	760
Tru1I	TTAA	3 cut(s)	627, 755, 822
Tru9I	TTAA	3 cut(s)	627, 755, 822
TscAI	CASTG	1 cut(s)	127
TseI	GCWGC	3 cut(s)	80, 782, 875
TspDTI	ATGAA	5 cut(s)	89, 207, 683, 773, 816
TspGWI	ACGGA	1 cut(s)	33
TspRI	CASTG	1 cut(s)	127
VpaK11BI	GGWCC	1 cut(s)	89
VspI	ATTAAT	1 cut(s)	755
XagI	CCTNNNNNAGG	1 cut(s)	410
XapI	RAATTY	1 cut(s)	743
XceI	RCATGY	1 cut(s)	132
XhoI	CTCGAG	1 cut(s)	489
ZrmI	AGTACT	1 cut(s)	466
Zsp2I	ATGCAT	1 cut(s)	130

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.