RchiOBHm_Chr6g0300991

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0007648
Physical Location & Seq
Forward (+)
61589892 .. 61592726
2835 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27030

Sequence Viewer

Length: 885 bp
ATGTTTGGTTACTGGGGAAAGTCTCATCATCAGGAGAATGATTCACTGCAGCCTGAGGTGAAGATCAACGAGCTAAAGGCTGCAATTGGACCCCTATCAGGCCGTAGTTTGCAGTTCTGTACTGATGCGTGCTTCAGGAGGTACTTGGATGCGCGGAATGGGAATGTAGACAAGTCGAAGAAAATGTTGGAAGAATCACTCAAATGGAGGTCGACTTATAAGCCTGAGGAAATTCGTTGGCCTGAAGTTGCAGTTGAAGGCGAGACTGGAAAAGTTTATAGAGCAAATTTTCGTGACCGTCATGGGAGGACTGTTCTGATATTGAGACCAGCAATGCAGAACACAATGTCATTAGACAATAACATGCGGCACTTGGTGTATCTTATAGAGAATGCAGTCTTTAATCTTCCAGAGGGTCAGGAACAAATGGCTTGGTTGATAGACTTTACTGGGTGGACACTGAATACTTCTGTGCCTGTCAAATCAGCACGCGATACCATCAATATTTTACAGAACCACTATCCTGAGAGGCTAGCCGTGGCGTTTCTCTACAACCCTCCACGGATTTTTGAAGCATTTTGGAAGATTGTCAAGTACTTCTTGGATAACAAAACATTCCACAAAGTAAAGTTTGTTTACCCGAAGAAGAAAGACAGCGTGGAGCTTATGAAACACTATTTTGATGAGGAAAATCTCCCTACTGAGTTTGGAGGAAAAGCCATTCTAGAGTATGACCACGAGGAGTTCTCAAAATTAATGACTCAAGATGATGTGAAATCAGCTGCCTTTTGGGGCTGTGATAACAAGTTACATCATGCTAGCAATGGCCATTCTGGAGCTGAGGTAGCTCCTGAACCAGCGTGCCTCACGCCGGCAGCTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

294

Amino Acids

33.98

Weight (kDa)

6.84

Isoelectric Point (pI)

51.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 89 - 238 7.7e-36 CRAL/TRIO domain
CRAL_TRIO_2 PF13716 103 - 247 5e-11 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 219
AccI GTMKAC 2 cut(s) 168, 212
AccII CGCG 2 cut(s) 154, 492
AciI CCGC 2 cut(s) 154, 367
AcoI YGGCCR 1 cut(s) 826
AcsI RAATTY 2 cut(s) 231, 286
AcuI CTGAAG 2 cut(s) 118, 264
AdeI CACNNNGTG 1 cut(s) 376
AfaI GTAC 3 cut(s) 121, 143, 596
AfiI CCNNNNNNNGG 2 cut(s) 98, 871
AgsI TTSAA 2 cut(s) 257, 572
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
AluBI AGCT 6 cut(s) 73, 664, 782, 839, 848, 878
AluI AGCT 6 cut(s) 73, 664, 782, 839, 848, 878
Alw26I GTCTC 3 cut(s) 27, 257, 319
AoxI GGCC 3 cut(s) 100, 239, 826
ApeKI GCWGC 4 cut(s) 49, 80, 782, 875
ApoI RAATTY 2 cut(s) 231, 286
AseI ATTAAT 1 cut(s) 755
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 1 cut(s) 89
AsuHPI GGTGA 1 cut(s) 70
AsuNHI GCTAGC 2 cut(s) 532, 818
AvaII GGWCC 1 cut(s) 89
AxyI CCTNAGG 2 cut(s) 54, 225
BalI TGGCCA 1 cut(s) 828
BauI CACGAG 1 cut(s) 737
BbvCI CCTCAGC 1 cut(s) 840
BbvI GCAGC 3 cut(s) 61, 67, 769
BccI CCATC 1 cut(s) 506
BceAI ACGGC 2 cut(s) 87, 521
BcoDI GTCTC 3 cut(s) 27, 257, 319
BfaI CTAG 4 cut(s) 533, 725, 819, 879
BfmI CTRYAG 1 cut(s) 47
BisI GCNGC 5 cut(s) 50, 81, 368, 783, 876
BlsI GCNGC 5 cut(s) 51, 82, 369, 784, 877
BmcAI AGTACT 1 cut(s) 596
Bme18I GGWCC 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 1 cut(s) 91
BmrI ACTGGG 2 cut(s) 22, 459
BmsI GCATC 2 cut(s) 115, 139
BmtI GCTAGC 2 cut(s) 536, 822
BmuI ACTGGG 2 cut(s) 22, 459
BplI GAGNNNNNCTC 2 cut(s) 731, 763
BpmI CTGGAG 1 cut(s) 855
Bpu10I CCTNAGC 1 cut(s) 840
BpuEI CTTGAG 1 cut(s) 747
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 2 cut(s) 537, 560
BsaXI ACNNNNNCTCC 2 cut(s) 828, 858
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 871
Bse118I RCCGGY 1 cut(s) 871
Bse1I ACTGG 3 cut(s) 17, 271, 454
Bse21I CCTNAGG 2 cut(s) 54, 225
Bse3DI GCAATG 2 cut(s) 339, 829
BseDI CCNNGG 2 cut(s) 537, 560
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 2 cut(s) 98, 871
BseMI GCAATG 2 cut(s) 339, 829
BseMII CTCAG 5 cut(s) 45, 216, 516, 693, 831
BseNI ACTGG 3 cut(s) 17, 271, 454
BseRI GAGGAG 1 cut(s) 755
BseXI GCAGC 3 cut(s) 61, 67, 769
Bsh1236I CGCG 2 cut(s) 154, 492
BshFI GGCC 3 cut(s) 102, 241, 828
BsiSI CCGG 1 cut(s) 872
BslI CCNNNNNNNGG 2 cut(s) 98, 871
BsmAI GTCTC 3 cut(s) 27, 257, 319
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 3 cut(s) 102, 241, 828
Bso31I GGTCTC 1 cut(s) 319
Bsp143I GATC 1 cut(s) 63
BspACI CCGC 2 cut(s) 154, 367
BspANI GGCC 3 cut(s) 102, 241, 828
BspCNI CTCAG 5 cut(s) 46, 217, 517, 694, 832
BspFNI CGCG 2 cut(s) 154, 492
BspLI GGNNCC 1 cut(s) 91
BspMAI CTGCAG 1 cut(s) 51
BspOI GCTAGC 2 cut(s) 536, 822
BspTNI GGTCTC 1 cut(s) 319
BsrDI GCAATG 2 cut(s) 339, 829
BsrFI RCCGGY 1 cut(s) 871
BsrI ACTGG 3 cut(s) 17, 271, 454
BssAI RCCGGY 1 cut(s) 871
BssECI CCNNGG 2 cut(s) 537, 560
BssMI GATC 1 cut(s) 63
BssSI CACGAG 1 cut(s) 737
Bst2BI CACGAG 1 cut(s) 737
Bst4CI ACNGT 2 cut(s) 299, 313
BstC8I GCNNGC 6 cut(s) 130, 490, 534, 820, 862, 873
BstDEI CTNAG 5 cut(s) 54, 225, 525, 702, 840
BstDSI CCRYGG 2 cut(s) 537, 560
BstF5I GGATG 1 cut(s) 154
BstFNI CGCG 2 cut(s) 154, 492
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 1 cut(s) 66
BstMAI GTCTC 3 cut(s) 27, 257, 319
BstMBI GATC 1 cut(s) 63
BstMWI GCNNNNNNNGC 1 cut(s) 845
BstNSI RCATGY 1 cut(s) 367
BstSFI CTRYAG 1 cut(s) 47
BstUI CGCG 2 cut(s) 154, 492
BstV1I GCAGC 3 cut(s) 61, 67, 769
Bsu36I CCTNAGG 2 cut(s) 54, 225
BsuRI GGCC 3 cut(s) 102, 241, 828
BtgI CCRYGG 2 cut(s) 537, 560
BtsCI GGATG 1 cut(s) 154
BtsI GCAGTG 1 cut(s) 44
BtsIMutI CAGTG 2 cut(s) 44, 458
Cac8I GCNNGC 6 cut(s) 130, 490, 534, 820, 862, 873
CfoI GCGC 1 cut(s) 154
Cfr10I RCCGGY 1 cut(s) 871
Cfr13I GGNCC 1 cut(s) 89
Csp6I GTAC 3 cut(s) 120, 142, 595
CviAII CATG 3 cut(s) 302, 364, 815
CviQI GTAC 3 cut(s) 120, 142, 595
DdeI CTNAG 5 cut(s) 54, 225, 525, 702, 840
DpnI GATC 1 cut(s) 65
DpnII GATC 1 cut(s) 63
DraIII CACNNNGTG 1 cut(s) 376
EaeI YGGCCR 1 cut(s) 826
Eco31I GGTCTC 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 89
Eco57I CTGAAG 2 cut(s) 118, 264
Eco81I CCTNAGG 2 cut(s) 54, 225
FaeI CATG 3 cut(s) 305, 367, 818
FaiI YATR 8 cut(s) 219, 279, 303, 365, 386, 668, 732, 816
FalI AAGNNNNNCTT 2 cut(s) 584, 616
FatI CATG 3 cut(s) 301, 363, 814
FblI GTMKAC 2 cut(s) 168, 212
Fnu4HI GCNGC 5 cut(s) 50, 81, 368, 783, 876
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 5 cut(s) 50, 81, 368, 783, 876
FspBI CTAG 4 cut(s) 533, 725, 819, 879
GlaI GCGC 1 cut(s) 153
GluI GCNGC 5 cut(s) 50, 81, 368, 783, 876
GsuI CTGGAG 1 cut(s) 855
HaeIII GGCC 3 cut(s) 102, 241, 828
HapII CCGG 1 cut(s) 872
HhaI GCGC 1 cut(s) 154
Hin1II CATG 3 cut(s) 305, 367, 818
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HinfI GANTC 3 cut(s) 41, 194, 760
HpaII CCGG 1 cut(s) 872
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 4 cut(s) 169, 213, 456, 637
Hpy188I TCNGA 1 cut(s) 318
Hpy8I GTNNAC 4 cut(s) 169, 213, 456, 637
HpyAV CCTTC 1 cut(s) 251
HpyCH4III ACNGT 2 cut(s) 299, 313
HpyCH4V TGCA 6 cut(s) 49, 83, 112, 251, 337, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 845
HpyF3I CTNAG 5 cut(s) 54, 225, 525, 702, 840
Hsp92II CATG 3 cut(s) 305, 367, 818
HspAI GCGC 1 cut(s) 152
KroI GCCGGC 1 cut(s) 871
KroNI GCCGGC 1 cut(s) 873
Kzo9I GATC 1 cut(s) 63
LmnI GCTCC 3 cut(s) 661, 836, 853
Lsp1109I GCAGC 3 cut(s) 61, 67, 769
LweI GCATC 2 cut(s) 115, 139
MaeI CTAG 4 cut(s) 533, 725, 819, 879
MaeIII GTNAC 3 cut(s) 8, 293, 807
MalI GATC 1 cut(s) 65
MboI GATC 1 cut(s) 63
MboII GAAGA 7 cut(s) 73, 190, 203, 398, 595, 655, 658
MfeI CAATTG 1 cut(s) 84
MlsI TGGCCA 1 cut(s) 828
MluCI AATT 4 cut(s) 84, 231, 286, 752
MluNI TGGCCA 1 cut(s) 828
MlyI GAGTC 1 cut(s) 754
MmeI TCCRAC 1 cut(s) 168
Mox20I TGGCCA 1 cut(s) 828
MroNI GCCGGC 1 cut(s) 871
MscI TGGCCA 1 cut(s) 828
MseI TTAA 2 cut(s) 402, 755
MslI CAYNNNNRTG 1 cut(s) 202
Msp20I TGGCCA 1 cut(s) 828
MspA1I CMGCKG 1 cut(s) 782
MspI CCGG 1 cut(s) 872
MunI CAATTG 1 cut(s) 84
Mva1269I GAATGC 1 cut(s) 397
MvnI CGCG 2 cut(s) 154, 492
MwoI GCNNNNNNNGC 1 cut(s) 845
NaeI GCCGGC 1 cut(s) 873
NdeII GATC 1 cut(s) 63
NgoMIV GCCGGC 1 cut(s) 871
NheI GCTAGC 2 cut(s) 532, 818
NlaIII CATG 3 cut(s) 305, 367, 818
NlaIV GGNNCC 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 293
NspI RCATGY 1 cut(s) 367
PctI GAATGC 1 cut(s) 397
PdiI GCCGGC 1 cut(s) 873
PfeI GAWTC 2 cut(s) 41, 194
PkrI GCNGC 5 cut(s) 51, 82, 369, 784, 877
PleI GAGTC 1 cut(s) 754
PpsI GAGTC 1 cut(s) 754
PshBI ATTAAT 1 cut(s) 755
PsiI TTATAA 1 cut(s) 219
PspN4I GGNNCC 1 cut(s) 91
PspPI GGNCC 1 cut(s) 89
PstI CTGCAG 1 cut(s) 51
PvuII CAGCTG 1 cut(s) 782
RsaI GTAC 3 cut(s) 121, 143, 596
RsaNI GTAC 3 cut(s) 120, 142, 595
RseI CAYNNNNRTG 1 cut(s) 202
SalI GTCGAC 1 cut(s) 211
SaqAI TTAA 2 cut(s) 402, 755
SatI GCNGC 5 cut(s) 50, 81, 368, 783, 876
Sau3AI GATC 1 cut(s) 63
Sau96I GGNCC 1 cut(s) 89
ScaI AGTACT 1 cut(s) 596
SchI GAGTC 1 cut(s) 754
SfaNI GCATC 2 cut(s) 115, 139
SfcI CTRYAG 1 cut(s) 47
SinI GGWCC 1 cut(s) 89
SmiMI CAYNNNNRTG 1 cut(s) 202
SmlI CTYRAG 1 cut(s) 762
SmoI CTYRAG 1 cut(s) 762
Sse9I AATT 4 cut(s) 84, 231, 286, 752
SsiI CCGC 2 cut(s) 154, 367
SspI AATATT 1 cut(s) 505
SspMI CTAG 4 cut(s) 533, 725, 819, 879
TaaI ACNGT 2 cut(s) 299, 313
TaqI TCGA 2 cut(s) 176, 212
TasI AATT 4 cut(s) 84, 231, 286, 752
TatI WGTACW 2 cut(s) 119, 594
TauI GCSGC 1 cut(s) 370
TfiI GAWTC 2 cut(s) 41, 194
Tru1I TTAA 2 cut(s) 402, 755
Tru9I TTAA 2 cut(s) 402, 755
TscAI CASTG 2 cut(s) 51, 465
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 4 cut(s) 49, 80, 782, 875
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 1 cut(s) 683
TspGWI ACGGA 1 cut(s) 577
TspRI CASTG 2 cut(s) 51, 465
VpaK11BI GGWCC 1 cut(s) 89
VspI ATTAAT 1 cut(s) 755
XapI RAATTY 2 cut(s) 231, 286
XbaI TCTAGA 1 cut(s) 724
XceI RCATGY 1 cut(s) 367
XmiI GTMKAC 2 cut(s) 168, 212
XspI CTAG 4 cut(s) 533, 725, 819, 879
ZrmI AGTACT 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.