Prupe.1G379800_v2.0.a1

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0007648
Physical Location & Seq
Reverse (-)
34282138 .. 34285658
3521 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G379800.3

Sequence Viewer

Length: 885 bp
ATGTTTCGCCTCTGGAGCAGTTCCCATCACCAAGACAATGATCCCTTGCAGCATGAAGTGAAGATCAATGAGCTGAAGGCTGCAATTGGACCGCTATCCGGGCGTAGTTTACAGTTCTGCACTGATGCGTGCTTCAGGAGATATTTGGAGGCACGGAATTGGAACGTAGACAAAGCCAAGAAAATGTTGGAAGAAACACTTAATTGGAGATCAACCTACAAGCCTGAGGAAATCTGCTGGCGCGAAGTTGCAAATGAGGGTGAGACTGGAAAAGTTTACAGAGCAAGTTTTCGTGATCGTAGCGGGAGGACTGTTCTTATACTGAGACCAGGAATGCAGAACACGGTATCAATAGACAATCAAATGCGTCATCTAGTCTATCTCATAGAGAATGCTGTCTTCAACCTTCCTGAGGGCCAAGAACAGATGGCGTGGTTGATAGACTTCACCGGGTGGACGCTAAGTACTACCGTGCCTGTCAAATCTGCACGAGACACCATCAATATTTTACAGAACCACTACCCTGAGAGATTAGCCGTAGCATTTCTCTACAACCCCCCGCGGATTTTTGAAGCATTTTGGAAGATTGTCAAGTACTTCTTGGACAACAAGACATTCCACAAGGTCAAGTTTGTTTACCCCAAGAATAAAGACAGCGTGGAGCTTATGAAGCAATATTTTGATGAGAAAAATCTCCCTACTGAGTTTGGAGGGCATGCCATACTAAACTATGACCATGAGGAGTTCTCGAAATTAATGACTCAAGATGATGTGAGATCAGCTGCCTTTTGGGGTCGCGATGACAAGCTACCACATTATGGTAACGGGCATTCTGGAGCCGAGGTGGCTCCTGAACCGGTGTGCCTTGCACCGGTAGCCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

295

Amino Acids

34.18

Weight (kDa)

6.8

Isoelectric Point (pI)

51.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 818
AccI GTMKAC 1 cut(s) 168
AccII CGCG 3 cut(s) 243, 562, 798
AciI CCGC 4 cut(s) 92, 303, 560, 562
AclWI GGATC 1 cut(s) 35
AcuI CTGAAG 2 cut(s) 95, 118
AdeI CACNNNGTG 1 cut(s) 453
AfaI GTAC 2 cut(s) 466, 596
AfiI CCNNNNNNNGG 4 cut(s) 98, 412, 818, 871
AgeI ACCGGT 2 cut(s) 856, 871
AgsI TTSAA 2 cut(s) 403, 572
AjnI CCWGG 1 cut(s) 328
AluBI AGCT 5 cut(s) 73, 664, 782, 808, 882
AluI AGCT 5 cut(s) 73, 664, 782, 808, 882
Alw26I GTCTC 3 cut(s) 257, 319, 486
AlwI GGATC 1 cut(s) 35
AoxI GGCC 1 cut(s) 415
ApeKI GCWGC 3 cut(s) 49, 80, 782
AseI ATTAAT 1 cut(s) 755
AsiGI ACCGGT 2 cut(s) 856, 871
AspLEI GCGC 1 cut(s) 243
AspS9I GGNCC 2 cut(s) 89, 415
AsuC2I CCSGG 2 cut(s) 100, 451
AsuHPI GGTGA 3 cut(s) 20, 272, 439
AvaII GGWCC 1 cut(s) 89
AxyI CCTNAGG 2 cut(s) 225, 411
BauI CACGAG 1 cut(s) 489
BbsI GAAGAC 1 cut(s) 391
BbvI GCAGC 3 cut(s) 61, 67, 769
BccI CCATC 3 cut(s) 33, 421, 506
BceAI ACGGC 1 cut(s) 521
BciT130I CCWGG 1 cut(s) 330
BcnI CCSGG 2 cut(s) 100, 451
BcoDI GTCTC 3 cut(s) 257, 319, 486
BfaI CTAG 1 cut(s) 374
BglI GCCNNNNNGGC 1 cut(s) 845
BisI GCNGC 3 cut(s) 50, 81, 783
BlsI GCNGC 3 cut(s) 51, 82, 784
BmcAI AGTACT 2 cut(s) 466, 596
Bme1390I CCNGG 3 cut(s) 100, 330, 451
Bme18I GGWCC 1 cut(s) 89
BmgT120I GGNCC 2 cut(s) 89, 415
BmiI GGNNCC 2 cut(s) 838, 849
BmrFI CCNGG 3 cut(s) 100, 330, 451
BmsI GCATC 1 cut(s) 115
BpiI GAAGAC 1 cut(s) 391
BplI GAGNNNNNCTC 2 cut(s) 731, 763
BpmI CTGGAG 2 cut(s) 34, 855
BpuEI CTTGAG 1 cut(s) 747
BpuMI CCSGG 2 cut(s) 100, 451
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 2 cut(s) 560, 840
BsaWI WCCGGW 2 cut(s) 856, 871
BsaXI ACNNNNNCTCC 2 cut(s) 828, 858
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 412, 818, 871
Bse118I RCCGGY 2 cut(s) 856, 871
Bse1I ACTGG 1 cut(s) 271
Bse21I CCTNAGG 2 cut(s) 225, 411
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 2 cut(s) 560, 840
BseLI CCNNNNNNNGG 4 cut(s) 98, 412, 818, 871
BseMII CTCAG 5 cut(s) 216, 314, 402, 516, 693
BseNI ACTGG 1 cut(s) 271
BseRI GAGGAG 1 cut(s) 755
BseXI GCAGC 3 cut(s) 61, 67, 769
BsgI GTGCAG 2 cut(s) 103, 471
Bsh1236I CGCG 3 cut(s) 243, 562, 798
BshFI GGCC 1 cut(s) 417
BshTI ACCGGT 2 cut(s) 856, 871
BsiSI CCGG 4 cut(s) 99, 450, 857, 872
BslI CCNNNNNNNGG 4 cut(s) 98, 412, 818, 871
BsmAI GTCTC 3 cut(s) 257, 319, 486
BsmI GAATGC 3 cut(s) 339, 397, 829
BsnI GGCC 1 cut(s) 417
Bso31I GGTCTC 1 cut(s) 319
Bsp143I GATC 5 cut(s) 40, 63, 209, 295, 776
Bsp68I TCGCGA 1 cut(s) 798
BspACI CCGC 4 cut(s) 92, 303, 560, 562
BspANI GGCC 1 cut(s) 417
BspCNI CTCAG 5 cut(s) 217, 315, 403, 517, 694
BspFNI CGCG 3 cut(s) 243, 562, 798
BspLI GGNNCC 2 cut(s) 838, 849
BspPI GGATC 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 319
BsrFI RCCGGY 2 cut(s) 856, 871
BsrI ACTGG 1 cut(s) 271
BssAI RCCGGY 2 cut(s) 856, 871
BssECI CCNNGG 2 cut(s) 560, 840
BssMI GATC 5 cut(s) 40, 63, 209, 295, 776
BssSI CACGAG 1 cut(s) 489
Bst2BI CACGAG 1 cut(s) 489
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 4 cut(s) 114, 313, 346, 472
BstC8I GCNNGC 4 cut(s) 130, 239, 717, 880
BstDEI CTNAG 6 cut(s) 225, 323, 411, 461, 525, 702
BstDSI CCRYGG 1 cut(s) 560
BstENI CCTNNNNNAGG 1 cut(s) 410
BstFNI CGCG 3 cut(s) 243, 562, 798
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 5 cut(s) 43, 66, 212, 298, 779
BstMAI GTCTC 3 cut(s) 257, 319, 486
BstMBI GATC 5 cut(s) 40, 63, 209, 295, 776
BstMWI GCNNNNNNNGC 5 cut(s) 15, 100, 670, 845, 875
BstNI CCWGG 1 cut(s) 330
BstNSI RCATGY 1 cut(s) 719
BstSCI CCNGG 3 cut(s) 98, 328, 449
BstUI CGCG 3 cut(s) 243, 562, 798
BstV1I GCAGC 3 cut(s) 61, 67, 769
BstV2I GAAGAC 1 cut(s) 391
Bsu36I CCTNAGG 2 cut(s) 225, 411
BsuRI GGCC 1 cut(s) 417
BtgI CCRYGG 1 cut(s) 560
BtgZI GCGATG 1 cut(s) 813
BtsIMutI CAGTG 1 cut(s) 120
BtuMI TCGCGA 1 cut(s) 798
Cac8I GCNNGC 4 cut(s) 130, 239, 717, 880
CfoI GCGC 1 cut(s) 243
Cfr10I RCCGGY 2 cut(s) 856, 871
Cfr13I GGNCC 2 cut(s) 89, 415
Cfr42I CCGCGG 1 cut(s) 563
CseI GACGC 2 cut(s) 356, 466
Csp6I GTAC 2 cut(s) 465, 595
CspAI ACCGGT 2 cut(s) 856, 871
CviAII CATG 3 cut(s) 53, 716, 737
CviQI GTAC 2 cut(s) 465, 595
DdeI CTNAG 6 cut(s) 225, 323, 411, 461, 525, 702
DpnI GATC 5 cut(s) 42, 65, 211, 297, 778
DpnII GATC 5 cut(s) 40, 63, 209, 295, 776
DraIII CACNNNGTG 1 cut(s) 453
Eco31I GGTCTC 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 89
Eco57I CTGAAG 2 cut(s) 95, 118
Eco81I CCTNAGG 2 cut(s) 225, 411
EcoNI CCTNNNNNAGG 1 cut(s) 410
EcoRII CCWGG 1 cut(s) 328
FaeI CATG 3 cut(s) 56, 719, 740
FaiI YATR 9 cut(s) 54, 320, 386, 668, 717, 722, 732, 738, 819
FalI AAGNNNNNCTT 4 cut(s) 183, 215, 584, 616
FatI CATG 3 cut(s) 52, 715, 736
FauI CCCGC 2 cut(s) 296, 567
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 3 cut(s) 50, 81, 783
Fsp4HI GCNGC 3 cut(s) 50, 81, 783
FspBI CTAG 1 cut(s) 374
GlaI GCGC 1 cut(s) 242
GluI GCNGC 3 cut(s) 50, 81, 783
GsuI CTGGAG 2 cut(s) 34, 855
HaeIII GGCC 1 cut(s) 417
HapII CCGG 4 cut(s) 99, 450, 857, 872
HgaI GACGC 2 cut(s) 356, 466
HhaI GCGC 1 cut(s) 243
Hin1II CATG 3 cut(s) 56, 719, 740
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HinfI GANTC 1 cut(s) 760
HpaII CCGG 4 cut(s) 99, 450, 857, 872
HphI GGTGA 3 cut(s) 20, 272, 439
Hpy166II GTNNAC 5 cut(s) 110, 169, 277, 456, 637
Hpy188III TCNNGA 9 cut(s) 13, 136, 293, 410, 748, 764, 797, 834, 851
Hpy8I GTNNAC 5 cut(s) 110, 169, 277, 456, 637
HpyAV CCTTC 2 cut(s) 70, 416
HpyCH4III ACNGT 4 cut(s) 114, 313, 346, 472
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 7 cut(s) 49, 83, 120, 251, 337, 488, 869
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 100, 670, 845, 875
HpyF3I CTNAG 6 cut(s) 225, 323, 411, 461, 525, 702
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 3 cut(s) 56, 719, 740
HspAI GCGC 1 cut(s) 241
KspI CCGCGG 1 cut(s) 563
Kzo9I GATC 5 cut(s) 40, 63, 209, 295, 776
LmnI GCTCC 4 cut(s) 15, 661, 836, 853
Lsp1109I GCAGC 3 cut(s) 61, 67, 769
LweI GCATC 1 cut(s) 115
MaeI CTAG 1 cut(s) 374
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 1 cut(s) 821
MalI GATC 5 cut(s) 42, 65, 211, 297, 778
MboI GATC 5 cut(s) 40, 63, 209, 295, 776
MboII GAAGA 4 cut(s) 73, 203, 391, 595
MfeI CAATTG 1 cut(s) 84
MluCI AATT 4 cut(s) 84, 157, 202, 752
MlyI GAGTC 1 cut(s) 754
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 9 cut(s) 20, 142, 220, 250, 300, 406, 704, 733, 835
MseI TTAA 2 cut(s) 201, 755
MspA1I CMGCKG 3 cut(s) 562, 782, 882
MspI CCGG 4 cut(s) 99, 450, 857, 872
MspR9I CCNGG 3 cut(s) 100, 330, 451
MunI CAATTG 1 cut(s) 84
Mva1269I GAATGC 3 cut(s) 339, 397, 829
MvaI CCWGG 1 cut(s) 330
MvnI CGCG 3 cut(s) 243, 562, 798
MwoI GCNNNNNNNGC 5 cut(s) 15, 100, 670, 845, 875
NciI CCSGG 2 cut(s) 100, 451
NdeII GATC 5 cut(s) 40, 63, 209, 295, 776
NlaIII CATG 3 cut(s) 56, 719, 740
NlaIV GGNNCC 2 cut(s) 838, 849
NmeAIII GCCGAG 1 cut(s) 865
NruI TCGCGA 1 cut(s) 798
NspI RCATGY 1 cut(s) 719
PaeI GCATGC 1 cut(s) 719
PctI GAATGC 3 cut(s) 339, 397, 829
PflMI CCANNNNNTGG 1 cut(s) 818
PinAI ACCGGT 2 cut(s) 856, 871
PkrI GCNGC 3 cut(s) 51, 82, 784
PleI GAGTC 1 cut(s) 754
PpsI GAGTC 1 cut(s) 754
PshBI ATTAAT 1 cut(s) 755
Psp6I CCWGG 1 cut(s) 328
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 2 cut(s) 838, 849
PspPI GGNCC 2 cut(s) 89, 415
PvuII CAGCTG 2 cut(s) 782, 882
RruI TCGCGA 1 cut(s) 798
RsaI GTAC 2 cut(s) 466, 596
RsaNI GTAC 2 cut(s) 465, 595
SacII CCGCGG 1 cut(s) 563
SaqAI TTAA 2 cut(s) 201, 755
SatI GCNGC 3 cut(s) 50, 81, 783
Sau3AI GATC 5 cut(s) 40, 63, 209, 295, 776
Sau96I GGNCC 2 cut(s) 89, 415
ScaI AGTACT 2 cut(s) 466, 596
SchI GAGTC 1 cut(s) 754
ScrFI CCNGG 3 cut(s) 100, 330, 451
SfaNI GCATC 1 cut(s) 115
Sfr303I CCGCGG 1 cut(s) 563
SgrBI CCGCGG 1 cut(s) 563
SinI GGWCC 1 cut(s) 89
SmlI CTYRAG 1 cut(s) 762
SmoI CTYRAG 1 cut(s) 762
SphI GCATGC 1 cut(s) 719
Sse9I AATT 4 cut(s) 84, 157, 202, 752
SsiI CCGC 4 cut(s) 92, 303, 560, 562
SspI AATATT 2 cut(s) 505, 677
SspMI CTAG 1 cut(s) 374
StyD4I CCNGG 3 cut(s) 98, 328, 449
TaaI ACNGT 4 cut(s) 114, 313, 346, 472
TaiI ACGT 1 cut(s) 168
TaqI TCGA 1 cut(s) 749
TasI AATT 4 cut(s) 84, 157, 202, 752
TatI WGTACW 2 cut(s) 464, 594
Tru1I TTAA 2 cut(s) 201, 755
Tru9I TTAA 2 cut(s) 201, 755
TscAI CASTG 1 cut(s) 127
TseI GCWGC 3 cut(s) 49, 80, 782
TspDTI ATGAA 2 cut(s) 69, 683
TspGWI ACGGA 1 cut(s) 169
TspRI CASTG 1 cut(s) 127
Van91I CCANNNNNTGG 1 cut(s) 818
VpaK11BI GGWCC 1 cut(s) 89
VspI ATTAAT 1 cut(s) 755
XagI CCTNNNNNAGG 1 cut(s) 410
XceI RCATGY 1 cut(s) 719
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XmiI GTMKAC 1 cut(s) 168
XspI CTAG 1 cut(s) 374
ZrmI AGTACT 2 cut(s) 466, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.