Rmu_co8375471.1_g000001

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8375471.1
OG0007648
Physical Location & Seq
Reverse (-)
2 .. 1076
1075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8375471.1_g000001.1.cds

Sequence Viewer

Length: 511 bp
atgcagaacacaatgtcattagacaataacatccggcacttggtgtatcttatagagaatgctgtctttaatcttccagagggtcaggaacaaatggcttggttgatagactttaccgggtggacactgaatacttctgtgcctgtcaaatcagcacgcgataccatcaatattttacagaaccactatcctgagaggctagccgtggcgtttctctacaaccctccacggatttttgaagcattttggaagattgtcaagtacttcttggataacaaaacattccacaaagtaaagtttgtttacccgaagaagaaagacagcgtggagcttatgaaacactattttgatgaggaaaatctccctactgagtttggaggaaaagccattctagagtatgaccacgaggagttctcaaaattaatgactcaagatgatgtgaaatcagctgccttttggggctgtgataacaagatacatcatactagcaatggccattctggagctgagg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

170

Amino Acids

19.89

Weight (kDa)

5.89

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 159
AcoI YGGCCR 1 cut(s) 493
AdeI CACNNNGTG 1 cut(s) 43
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 1 cut(s) 40
AgsI TTSAA 1 cut(s) 239
AluBI AGCT 3 cut(s) 331, 449, 506
AluI AGCT 3 cut(s) 331, 449, 506
AoxI GGCC 1 cut(s) 493
ApeKI GCWGC 1 cut(s) 449
AseI ATTAAT 1 cut(s) 422
AsuC2I CCSGG 1 cut(s) 118
AsuNHI GCTAGC 1 cut(s) 199
BalI TGGCCA 1 cut(s) 495
BauI CACGAG 1 cut(s) 404
BbvCI CCTCAGC 1 cut(s) 507
BbvI GCAGC 1 cut(s) 436
BccI CCATC 1 cut(s) 173
BceAI ACGGC 1 cut(s) 188
BcnI CCSGG 1 cut(s) 118
BfaI CTAG 3 cut(s) 200, 392, 486
BisI GCNGC 1 cut(s) 450
BlsI GCNGC 1 cut(s) 451
BmcAI AGTACT 1 cut(s) 263
Bme1390I CCNGG 1 cut(s) 118
BmrFI CCNGG 1 cut(s) 118
BmtI GCTAGC 1 cut(s) 203
BplI GAGNNNNNCTC 2 cut(s) 398, 430
Bpu10I CCTNAGC 1 cut(s) 507
BpuEI CTTGAG 1 cut(s) 414
BpuMI CCSGG 1 cut(s) 118
BsaJI CCNNGG 2 cut(s) 204, 227
Bsc4I CCNNNNNNNGG 1 cut(s) 40
Bse3DI GCAATG 1 cut(s) 496
BseDI CCNNGG 2 cut(s) 204, 227
BseGI GGATG 1 cut(s) 30
BseLI CCNNNNNNNGG 1 cut(s) 40
BseMI GCAATG 1 cut(s) 496
BseMII CTCAG 3 cut(s) 183, 360, 498
BseRI GAGGAG 1 cut(s) 422
BseXI GCAGC 1 cut(s) 436
Bsh1236I CGCG 1 cut(s) 159
BshFI GGCC 1 cut(s) 495
BsiSI CCGG 2 cut(s) 34, 117
BslI CCNNNNNNNGG 1 cut(s) 40
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 1 cut(s) 495
BspANI GGCC 1 cut(s) 495
BspCNI CTCAG 3 cut(s) 184, 361, 499
BspFNI CGCG 1 cut(s) 159
BspOI GCTAGC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 496
BssECI CCNNGG 2 cut(s) 204, 227
BssSI CACGAG 1 cut(s) 404
Bst2BI CACGAG 1 cut(s) 404
BstC8I GCNNGC 2 cut(s) 157, 201
BstDEI CTNAG 3 cut(s) 192, 369, 507
BstDSI CCRYGG 2 cut(s) 204, 227
BstF5I GGATG 1 cut(s) 30
BstFNI CGCG 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 116
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 436
BsuRI GGCC 1 cut(s) 495
BtgI CCRYGG 2 cut(s) 204, 227
BtsCI GGATG 1 cut(s) 30
BtsIMutI CAGTG 1 cut(s) 125
Cac8I GCNNGC 2 cut(s) 157, 201
Csp6I GTAC 1 cut(s) 262
CviJI RGCY 9 cut(s) 98, 199, 203, 331, 386, 449, 462, 495, 506
CviKI_1 RGCY 9 cut(s) 98, 199, 203, 331, 386, 449, 462, 495, 506
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 3 cut(s) 192, 369, 507
DraIII CACNNNGTG 1 cut(s) 43
EaeI YGGCCR 1 cut(s) 493
FaiI YATR 4 cut(s) 53, 335, 399, 483
FalI AAGNNNNNCTT 2 cut(s) 251, 283
Fnu4HI GCNGC 1 cut(s) 450
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 1 cut(s) 450
FspBI CTAG 3 cut(s) 200, 392, 486
GluI GCNGC 1 cut(s) 450
HaeIII GGCC 1 cut(s) 495
HapII CCGG 2 cut(s) 34, 117
HinfI GANTC 1 cut(s) 427
HpaII CCGG 2 cut(s) 34, 117
Hpy166II GTNNAC 2 cut(s) 123, 304
Hpy188III TCNNGA 6 cut(s) 77, 86, 191, 392, 431, 501
Hpy8I GTNNAC 2 cut(s) 123, 304
HpyCH4V TGCA 1 cut(s) 4
HpyF3I CTNAG 3 cut(s) 192, 369, 507
LmnI GCTCC 2 cut(s) 328, 503
LpnPI CCDG 7 cut(s) 47, 71, 90, 130, 156, 204, 486
Lsp1109I GCAGC 1 cut(s) 436
MaeI CTAG 3 cut(s) 200, 392, 486
MboII GAAGA 4 cut(s) 65, 262, 322, 325
MlsI TGGCCA 1 cut(s) 495
MluCI AATT 1 cut(s) 419
MluNI TGGCCA 1 cut(s) 495
MlyI GAGTC 1 cut(s) 421
MnlI CCTC 7 cut(s) 73, 189, 234, 346, 371, 400, 502
Mox20I TGGCCA 1 cut(s) 495
MscI TGGCCA 1 cut(s) 495
MseI TTAA 2 cut(s) 69, 422
Msp20I TGGCCA 1 cut(s) 495
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 2 cut(s) 34, 117
MspR9I CCNGG 1 cut(s) 118
Mva1269I GAATGC 1 cut(s) 64
MvnI CGCG 1 cut(s) 159
NciI CCSGG 1 cut(s) 118
NheI GCTAGC 1 cut(s) 199
PctI GAATGC 1 cut(s) 64
PkrI GCNGC 1 cut(s) 451
PleI GAGTC 1 cut(s) 421
PpsI GAGTC 1 cut(s) 421
PshBI ATTAAT 1 cut(s) 422
PvuII CAGCTG 1 cut(s) 449
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
SaqAI TTAA 2 cut(s) 69, 422
SatI GCNGC 1 cut(s) 450
ScaI AGTACT 1 cut(s) 263
SchI GAGTC 1 cut(s) 421
ScrFI CCNGG 1 cut(s) 118
SetI ASST 3 cut(s) 333, 451, 508
SmlI CTYRAG 1 cut(s) 429
SmoI CTYRAG 1 cut(s) 429
Sse9I AATT 1 cut(s) 419
SspI AATATT 1 cut(s) 172
SspMI CTAG 3 cut(s) 200, 392, 486
StyD4I CCNGG 1 cut(s) 116
TasI AATT 1 cut(s) 419
TatI WGTACW 1 cut(s) 261
Tru1I TTAA 2 cut(s) 69, 422
Tru9I TTAA 2 cut(s) 69, 422
TscAI CASTG 1 cut(s) 132
TseI GCWGC 1 cut(s) 449
TspDTI ATGAA 1 cut(s) 350
TspGWI ACGGA 1 cut(s) 244
TspRI CASTG 1 cut(s) 132
VspI ATTAAT 1 cut(s) 422
XbaI TCTAGA 1 cut(s) 391
XspI CTAG 3 cut(s) 200, 392, 486
ZrmI AGTACT 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.