Rh6DG413600

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0007648
Physical Location & Seq
Forward (+)
59863509 .. 59866303
2795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG413600.1

Sequence Viewer

Length: 885 bp
ATGTTTGGCTACTGGGGAAAGTCTCATCATCACGAGAATGATTCACTACAGCCTGAGGTGAAGATCAACGAGCTAAAGGCTGCAATTGGACCCCTATCAGGCCGTAGTTTGCAGTTCTGTACTGATGCGTGCTTCAGGAGGTACTTGGATGCGCGGAATGGGAATGTAGACAAGTCGAAGAAAATGTTGGAAGAAACACTCAAATGGAGGTCGACTTATAAGCCTGAGGAAATTCGTTGGCCTGAAGTTGCAGTTGAAGGCGAGACTGGAAAAGTTTATAGAGCAAATTTTCGTGACCGACATGGGAGGACTGTTCTGATATTGAGACCAGCAATGCAGAACACAATGTCATTAGACAATAACATCCGGCACTTGGTCTATCTTATAGAGAATGCTGTCTTTAATCTTCCAGAGGGTCAAGAACAAATGGCTTGGTTGATAGACTTTACCGGGTGGACACTGAATACTTCTGTCCCTGTCAAATCAGCACGCGATACCATCAATATTTTACAGAACCACTATCCTGAGAGGCTAGCCGTGGCGTTTCTCTACAACCCTCCACGGATTTTTGAAGCATTTTGGAAGATTGTCAAGTACTTCTTGGATAACAAAACATTCCACAAAGTAAAGTTTGTTTACCCGAAGAAGAAAGACAGCGTGGAGCTTATGAAACACTATTTTGATGAGGAAAATCTCCCTACTGAGTTTGGAGGAAAAGCCATTCTAGAGTATGACCACGAGGAGTTCTCAAAATTAATGGCTCAAGATGATGTGAAATCAGCTGCCTTTTGGGGCTGTGATAACAAGATACATCATGCTAGCAATGGTCATTCTGGAGCTGAGGTAGCTCCTGAACCAGCGTGCCTCACGCCGGCAGCTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

294

Amino Acids

33.95

Weight (kDa)

6.87

Isoelectric Point (pI)

53.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 89 - 238 1.1e-35 CRAL/TRIO domain
CRAL_TRIO_2 PF13716 103 - 247 1.5e-10 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 219
AccI GTMKAC 2 cut(s) 168, 212
AccII CGCG 2 cut(s) 154, 492
AciI CCGC 1 cut(s) 154
AcsI RAATTY 2 cut(s) 231, 286
AcuI CTGAAG 2 cut(s) 118, 264
AfaI GTAC 3 cut(s) 121, 143, 596
AfiI CCNNNNNNNGG 3 cut(s) 98, 373, 871
AgsI TTSAA 2 cut(s) 257, 572
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
AluBI AGCT 6 cut(s) 73, 664, 782, 839, 848, 878
AluI AGCT 6 cut(s) 73, 664, 782, 839, 848, 878
Alw26I GTCTC 3 cut(s) 27, 257, 319
AoxI GGCC 2 cut(s) 100, 239
ApeKI GCWGC 3 cut(s) 80, 782, 875
ApoI RAATTY 2 cut(s) 231, 286
AseI ATTAAT 1 cut(s) 755
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 1 cut(s) 89
AsuC2I CCSGG 1 cut(s) 451
AsuHPI GGTGA 1 cut(s) 70
AsuNHI GCTAGC 2 cut(s) 532, 818
AvaII GGWCC 1 cut(s) 89
AxyI CCTNAGG 2 cut(s) 54, 225
BauI CACGAG 2 cut(s) 32, 737
BbvCI CCTCAGC 1 cut(s) 840
BbvI GCAGC 2 cut(s) 67, 769
BccI CCATC 1 cut(s) 506
BceAI ACGGC 2 cut(s) 87, 521
BcnI CCSGG 1 cut(s) 451
BcoDI GTCTC 3 cut(s) 27, 257, 319
BfaI CTAG 4 cut(s) 533, 725, 819, 879
BfmI CTRYAG 1 cut(s) 47
BisI GCNGC 3 cut(s) 81, 783, 876
BlsI GCNGC 3 cut(s) 82, 784, 877
BmcAI AGTACT 1 cut(s) 596
Bme1390I CCNGG 1 cut(s) 451
Bme18I GGWCC 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 1 cut(s) 451
BmrI ACTGGG 1 cut(s) 22
BmsI GCATC 2 cut(s) 115, 139
BmtI GCTAGC 2 cut(s) 536, 822
BmuI ACTGGG 1 cut(s) 22
BplI GAGNNNNNCTC 2 cut(s) 731, 763
BpmI CTGGAG 1 cut(s) 855
Bpu10I CCTNAGC 1 cut(s) 840
BpuEI CTTGAG 1 cut(s) 747
BpuMI CCSGG 1 cut(s) 451
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 2 cut(s) 537, 560
BsaXI ACNNNNNCTCC 2 cut(s) 828, 858
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 373, 871
Bse118I RCCGGY 1 cut(s) 871
Bse1I ACTGG 2 cut(s) 17, 271
Bse21I CCTNAGG 2 cut(s) 54, 225
Bse3DI GCAATG 2 cut(s) 339, 829
BseDI CCNNGG 2 cut(s) 537, 560
BseGI GGATG 2 cut(s) 154, 363
BseLI CCNNNNNNNGG 3 cut(s) 98, 373, 871
BseMI GCAATG 2 cut(s) 339, 829
BseMII CTCAG 5 cut(s) 45, 216, 516, 693, 831
BseNI ACTGG 2 cut(s) 17, 271
BseRI GAGGAG 1 cut(s) 755
BseXI GCAGC 2 cut(s) 67, 769
Bsh1236I CGCG 2 cut(s) 154, 492
BshFI GGCC 2 cut(s) 102, 241
BsiSI CCGG 3 cut(s) 367, 450, 872
BslFI GGGAC 1 cut(s) 458
BslI CCNNNNNNNGG 3 cut(s) 98, 373, 871
BsmAI GTCTC 3 cut(s) 27, 257, 319
BsmFI GGGAC 1 cut(s) 458
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 2 cut(s) 102, 241
Bso31I GGTCTC 1 cut(s) 319
Bsp143I GATC 1 cut(s) 63
BspACI CCGC 1 cut(s) 154
BspANI GGCC 2 cut(s) 102, 241
BspCNI CTCAG 5 cut(s) 46, 217, 517, 694, 832
BspFNI CGCG 2 cut(s) 154, 492
BspLI GGNNCC 1 cut(s) 91
BspOI GCTAGC 2 cut(s) 536, 822
BspTNI GGTCTC 1 cut(s) 319
BsrDI GCAATG 2 cut(s) 339, 829
BsrFI RCCGGY 1 cut(s) 871
BsrI ACTGG 2 cut(s) 17, 271
BssAI RCCGGY 1 cut(s) 871
BssECI CCNNGG 2 cut(s) 537, 560
BssMI GATC 1 cut(s) 63
BssSI CACGAG 2 cut(s) 32, 737
Bst2BI CACGAG 2 cut(s) 32, 737
Bst4CI ACNGT 1 cut(s) 313
BstC8I GCNNGC 6 cut(s) 130, 490, 534, 820, 862, 873
BstDEI CTNAG 5 cut(s) 54, 225, 525, 702, 840
BstDSI CCRYGG 2 cut(s) 537, 560
BstF5I GGATG 2 cut(s) 154, 363
BstFNI CGCG 2 cut(s) 154, 492
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 1 cut(s) 66
BstMAI GTCTC 3 cut(s) 27, 257, 319
BstMBI GATC 1 cut(s) 63
BstMWI GCNNNNNNNGC 1 cut(s) 845
BstSCI CCNGG 1 cut(s) 449
BstSFI CTRYAG 1 cut(s) 47
BstUI CGCG 2 cut(s) 154, 492
BstV1I GCAGC 2 cut(s) 67, 769
Bsu36I CCTNAGG 2 cut(s) 54, 225
BsuRI GGCC 2 cut(s) 102, 241
BtgI CCRYGG 2 cut(s) 537, 560
BtsCI GGATG 2 cut(s) 154, 363
BtsIMutI CAGTG 1 cut(s) 458
Cac8I GCNNGC 6 cut(s) 130, 490, 534, 820, 862, 873
CfoI GCGC 1 cut(s) 154
Cfr10I RCCGGY 1 cut(s) 871
Cfr13I GGNCC 1 cut(s) 89
Csp6I GTAC 3 cut(s) 120, 142, 595
CviAII CATG 2 cut(s) 302, 815
CviQI GTAC 3 cut(s) 120, 142, 595
DdeI CTNAG 5 cut(s) 54, 225, 525, 702, 840
DpnI GATC 1 cut(s) 65
DpnII GATC 1 cut(s) 63
Eco31I GGTCTC 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 89
Eco57I CTGAAG 2 cut(s) 118, 264
Eco81I CCTNAGG 2 cut(s) 54, 225
FaeI CATG 2 cut(s) 305, 818
FaiI YATR 7 cut(s) 219, 279, 303, 386, 668, 732, 816
FalI AAGNNNNNCTT 2 cut(s) 584, 616
FaqI GGGAC 1 cut(s) 458
FatI CATG 2 cut(s) 301, 814
FblI GTMKAC 2 cut(s) 168, 212
Fnu4HI GCNGC 3 cut(s) 81, 783, 876
FokI GGATG 2 cut(s) 161, 350
Fsp4HI GCNGC 3 cut(s) 81, 783, 876
FspBI CTAG 4 cut(s) 533, 725, 819, 879
GlaI GCGC 1 cut(s) 153
GluI GCNGC 3 cut(s) 81, 783, 876
GsuI CTGGAG 1 cut(s) 855
HaeIII GGCC 2 cut(s) 102, 241
HapII CCGG 3 cut(s) 367, 450, 872
HhaI GCGC 1 cut(s) 154
Hin1II CATG 2 cut(s) 305, 818
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HinfI GANTC 1 cut(s) 41
HpaII CCGG 3 cut(s) 367, 450, 872
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 4 cut(s) 169, 213, 456, 637
Hpy188I TCNGA 1 cut(s) 318
Hpy8I GTNNAC 4 cut(s) 169, 213, 456, 637
HpyAV CCTTC 1 cut(s) 251
HpyCH4III ACNGT 1 cut(s) 313
HpyCH4V TGCA 4 cut(s) 83, 112, 251, 337
HpyF10VI GCNNNNNNNGC 1 cut(s) 845
HpyF3I CTNAG 5 cut(s) 54, 225, 525, 702, 840
Hsp92II CATG 2 cut(s) 305, 818
HspAI GCGC 1 cut(s) 152
KroI GCCGGC 1 cut(s) 871
KroNI GCCGGC 1 cut(s) 873
Kzo9I GATC 1 cut(s) 63
LmnI GCTCC 3 cut(s) 661, 836, 853
Lsp1109I GCAGC 2 cut(s) 67, 769
LweI GCATC 2 cut(s) 115, 139
MaeI CTAG 4 cut(s) 533, 725, 819, 879
MaeIII GTNAC 1 cut(s) 293
MalI GATC 1 cut(s) 65
MboI GATC 1 cut(s) 63
MboII GAAGA 7 cut(s) 73, 190, 203, 398, 595, 655, 658
MfeI CAATTG 1 cut(s) 84
MluCI AATT 4 cut(s) 84, 231, 286, 752
MmeI TCCRAC 1 cut(s) 168
MroNI GCCGGC 1 cut(s) 871
MseI TTAA 2 cut(s) 402, 755
MslI CAYNNNNRTG 2 cut(s) 36, 202
MspA1I CMGCKG 1 cut(s) 782
MspI CCGG 3 cut(s) 367, 450, 872
MspR9I CCNGG 1 cut(s) 451
MunI CAATTG 1 cut(s) 84
Mva1269I GAATGC 1 cut(s) 397
MvnI CGCG 2 cut(s) 154, 492
MwoI GCNNNNNNNGC 1 cut(s) 845
NaeI GCCGGC 1 cut(s) 873
NciI CCSGG 1 cut(s) 451
NdeII GATC 1 cut(s) 63
NgoMIV GCCGGC 1 cut(s) 871
NheI GCTAGC 2 cut(s) 532, 818
NlaIII CATG 2 cut(s) 305, 818
NlaIV GGNNCC 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 293
PctI GAATGC 1 cut(s) 397
PdiI GCCGGC 1 cut(s) 873
PfeI GAWTC 1 cut(s) 41
PkrI GCNGC 3 cut(s) 82, 784, 877
PshBI ATTAAT 1 cut(s) 755
PsiI TTATAA 1 cut(s) 219
PspN4I GGNNCC 1 cut(s) 91
PspPI GGNCC 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 782
RsaI GTAC 3 cut(s) 121, 143, 596
RsaNI GTAC 3 cut(s) 120, 142, 595
RseI CAYNNNNRTG 2 cut(s) 36, 202
SalI GTCGAC 1 cut(s) 211
SaqAI TTAA 2 cut(s) 402, 755
SatI GCNGC 3 cut(s) 81, 783, 876
Sau3AI GATC 1 cut(s) 63
Sau96I GGNCC 1 cut(s) 89
ScaI AGTACT 1 cut(s) 596
ScrFI CCNGG 1 cut(s) 451
SfaNI GCATC 2 cut(s) 115, 139
SfcI CTRYAG 1 cut(s) 47
SinI GGWCC 1 cut(s) 89
SmiMI CAYNNNNRTG 2 cut(s) 36, 202
SmlI CTYRAG 1 cut(s) 762
SmoI CTYRAG 1 cut(s) 762
Sse9I AATT 4 cut(s) 84, 231, 286, 752
SsiI CCGC 1 cut(s) 154
SspI AATATT 1 cut(s) 505
SspMI CTAG 4 cut(s) 533, 725, 819, 879
StyD4I CCNGG 1 cut(s) 449
TaaI ACNGT 1 cut(s) 313
TaqI TCGA 2 cut(s) 176, 212
TaqII GACCGA 1 cut(s) 312
TasI AATT 4 cut(s) 84, 231, 286, 752
TatI WGTACW 2 cut(s) 119, 594
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 2 cut(s) 402, 755
Tru9I TTAA 2 cut(s) 402, 755
TscAI CASTG 1 cut(s) 465
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 3 cut(s) 80, 782, 875
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 1 cut(s) 683
TspGWI ACGGA 1 cut(s) 577
TspRI CASTG 1 cut(s) 465
VpaK11BI GGWCC 1 cut(s) 89
VspI ATTAAT 1 cut(s) 755
XapI RAATTY 2 cut(s) 231, 286
XbaI TCTAGA 1 cut(s) 724
XmiI GTMKAC 2 cut(s) 168, 212
XspI CTAG 4 cut(s) 533, 725, 819, 879
ZrmI AGTACT 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.