Rmu_sc0011701.1_g000029

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0011701.1
OG0007648
Physical Location & Seq
Reverse (-)
94265 .. 95434
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0011701.1_g000029.1.cds

Sequence Viewer

Length: 432 bp
atgccaaaaccctcccgcccaatctgccattcccctaactccaatggaatcctagcaacctatctccatgcatcagatcgttccagaaatctccacagcacgagacctggttccttgatccggcgacccgcgaaagcaactcgactggaacttcttcgtctggtgacgacacgatggaaaactggtgtggtttggttcgtcttctcgccatggtttcacttccagcctcggaggtacttggatgcgtggaacgggaatgtagtcaagttgaagaaaatgttggaagaaacactcaaatggaggttgacttataagcctgaggaaattagttggcctgaagttgcagttgaaggcgagactggaaaagtttatagagcaaattttcgtgaccgttatgggagaactgttctgatattgagaccagcaatgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

143

Amino Acids

16.82

Weight (kDa)

10.79

Isoelectric Point (pI)

50.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 312
AccII CGCG 1 cut(s) 131
AciI CCGC 2 cut(s) 16, 129
AclWI GGATC 1 cut(s) 112
AcsI RAATTY 1 cut(s) 379
AcuI CTGAAG 1 cut(s) 357
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 1 cut(s) 120
AgsI TTSAA 2 cut(s) 271, 350
AjnI CCWGG 1 cut(s) 106
AjuI GAANNNNNNNTTGG 4 cut(s) 13, 45, 263, 295
Alw26I GTCTC 3 cut(s) 97, 350, 412
AlwI GGATC 1 cut(s) 112
AoxI GGCC 1 cut(s) 332
ApoI RAATTY 1 cut(s) 379
Asp700I GAANNNNTTC 1 cut(s) 153
AsuHPI GGTGA 1 cut(s) 175
AxyI CCTNAGG 1 cut(s) 318
BauI CACGAG 1 cut(s) 100
BbsI GAAGAC 1 cut(s) 193
BccI CCATC 1 cut(s) 168
BciT130I CCWGG 1 cut(s) 108
BcoDI GTCTC 3 cut(s) 97, 350, 412
BfaI CTAG 1 cut(s) 53
Bme1390I CCNGG 1 cut(s) 108
BmiI GGNNCC 1 cut(s) 112
BmrFI CCNGG 1 cut(s) 108
BmsI GCATC 2 cut(s) 80, 232
BpiI GAAGAC 1 cut(s) 193
BsaI GGTCTC 2 cut(s) 97, 412
BsaJI CCNNGG 2 cut(s) 209, 227
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse1I ACTGG 3 cut(s) 150, 187, 364
Bse21I CCTNAGG 1 cut(s) 318
Bse3DI GCAATG 1 cut(s) 432
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 2 cut(s) 209, 227
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 1 cut(s) 120
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 3 cut(s) 150, 187, 364
Bsh1236I CGCG 1 cut(s) 131
BshFI GGCC 1 cut(s) 334
BsiSI CCGG 1 cut(s) 121
BslI CCNNNNNNNGG 1 cut(s) 120
BsmAI GTCTC 3 cut(s) 97, 350, 412
BsnI GGCC 1 cut(s) 334
Bso31I GGTCTC 2 cut(s) 97, 412
Bsp143I GATC 2 cut(s) 76, 117
Bsp19I CCATGG 1 cut(s) 209
BspACI CCGC 2 cut(s) 16, 129
BspANI GGCC 1 cut(s) 334
BspCNI CTCAG 1 cut(s) 310
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 1 cut(s) 112
BspPI GGATC 1 cut(s) 112
BspTNI GGTCTC 2 cut(s) 97, 412
BsrDI GCAATG 1 cut(s) 432
BsrI ACTGG 3 cut(s) 150, 187, 364
BssECI CCNNGG 2 cut(s) 209, 227
BssMI GATC 2 cut(s) 76, 117
BssSI CACGAG 1 cut(s) 100
BssT1I CCWWGG 1 cut(s) 209
Bst2BI CACGAG 1 cut(s) 100
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 2 cut(s) 392, 406
BstDEI CTNAG 1 cut(s) 318
BstDSI CCRYGG 1 cut(s) 209
BstF5I GGATG 1 cut(s) 247
BstFNI CGCG 1 cut(s) 131
BstKTI GATC 2 cut(s) 79, 120
BstMAI GTCTC 3 cut(s) 97, 350, 412
BstMBI GATC 2 cut(s) 76, 117
BstMWI GCNNNNNNNGC 1 cut(s) 24
BstNI CCWGG 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 106
BstUI CGCG 1 cut(s) 131
BstV2I GAAGAC 1 cut(s) 193
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 1 cut(s) 334
BtgI CCRYGG 1 cut(s) 209
BtsCI GGATG 1 cut(s) 247
CsiI ACCWGGT 1 cut(s) 106
Csp6I GTAC 1 cut(s) 235
CviAII CATG 2 cut(s) 68, 210
CviJI RGCY 3 cut(s) 226, 316, 334
CviKI_1 RGCY 3 cut(s) 226, 316, 334
CviQI GTAC 1 cut(s) 235
DdeI CTNAG 1 cut(s) 318
DpnI GATC 2 cut(s) 78, 119
DpnII GATC 2 cut(s) 76, 117
Eco130I CCWWGG 1 cut(s) 209
Eco31I GGTCTC 2 cut(s) 97, 412
Eco57I CTGAAG 1 cut(s) 357
Eco81I CCTNAGG 1 cut(s) 318
EcoRII CCWGG 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 209
EcoT22I ATGCAT 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 209
FaeI CATG 2 cut(s) 71, 213
FaiI YATR 5 cut(s) 69, 211, 312, 372, 396
FatI CATG 2 cut(s) 67, 209
FauI CCCGC 2 cut(s) 23, 136
FokI GGATG 1 cut(s) 254
FspBI CTAG 1 cut(s) 53
HaeIII GGCC 1 cut(s) 334
HapII CCGG 1 cut(s) 121
Hin1II CATG 2 cut(s) 71, 213
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HinfI GANTC 1 cut(s) 48
HpaII CCGG 1 cut(s) 121
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 1 cut(s) 306
Hpy188I TCNGA 3 cut(s) 76, 231, 411
Hpy188III TCNNGA 2 cut(s) 84, 386
Hpy8I GTNNAC 1 cut(s) 306
HpyAV CCTTC 1 cut(s) 344
HpyCH4III ACNGT 2 cut(s) 392, 406
HpyCH4V TGCA 2 cut(s) 71, 344
HpyF10VI GCNNNNNNNGC 1 cut(s) 24
HpyF3I CTNAG 1 cut(s) 318
Hsp92II CATG 2 cut(s) 71, 213
Kzo9I GATC 2 cut(s) 76, 117
LweI GCATC 2 cut(s) 80, 232
MabI ACCWGGT 1 cut(s) 106
MaeI CTAG 1 cut(s) 53
MaeIII GTNAC 2 cut(s) 163, 386
MalI GATC 2 cut(s) 78, 119
MboI GATC 2 cut(s) 76, 117
MboII GAAGA 4 cut(s) 146, 193, 283, 296
MluCI AATT 2 cut(s) 324, 379
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 5 cut(s) 22, 225, 237, 294, 313
Mph1103I ATGCAT 1 cut(s) 73
MroXI GAANNNNTTC 1 cut(s) 153
MslI CAYNNNNRTG 1 cut(s) 295
MspI CCGG 1 cut(s) 121
MspR9I CCNGG 1 cut(s) 108
MvaI CCWGG 1 cut(s) 108
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 1 cut(s) 24
NcoI CCATGG 1 cut(s) 209
NdeII GATC 2 cut(s) 76, 117
NlaIII CATG 2 cut(s) 71, 213
NlaIV GGNNCC 1 cut(s) 112
NmuCI GTSAC 2 cut(s) 163, 386
NsiI ATGCAT 1 cut(s) 73
PdmI GAANNNNTTC 1 cut(s) 153
PfeI GAWTC 1 cut(s) 48
PsiI TTATAA 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 1 cut(s) 112
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
RseI CAYNNNNRTG 1 cut(s) 295
Sau3AI GATC 2 cut(s) 76, 117
ScrFI CCNGG 1 cut(s) 108
SetI ASST 4 cut(s) 62, 109, 236, 305
SexAI ACCWGGT 1 cut(s) 106
SfaNI GCATC 2 cut(s) 80, 232
SmiMI CAYNNNNRTG 1 cut(s) 295
Sse9I AATT 2 cut(s) 324, 379
SsiI CCGC 2 cut(s) 16, 129
SspMI CTAG 1 cut(s) 53
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 1 cut(s) 209
TaaI ACNGT 2 cut(s) 392, 406
TaqI TCGA 1 cut(s) 142
TasI AATT 2 cut(s) 324, 379
TfiI GAWTC 1 cut(s) 48
TseFI GTSAC 2 cut(s) 163, 386
Tsp45I GTSAC 2 cut(s) 163, 386
XapI RAATTY 1 cut(s) 379
XmnI GAANNNNTTC 1 cut(s) 153
XspI CTAG 1 cut(s) 53
Zsp2I ATGCAT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.