Rh6CG428200

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0007648
Physical Location & Seq
Forward (+)
60985300 .. 60988105
2806 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG428200.1

Sequence Viewer

Length: 885 bp
ATGTTTGGCTACTGGGGAAAGTCTCATCATCACGAGAATGATTCACTACAGCCTGAGGTGAAGATCAACGAGCTAAAGGCTGCAATTGGACCCCTATCAGGCCGTAGTTTGCAGTTCTGTACTGATGCGTGCTTCAGGAGGTACTTGGATGCGCGGAATGGGAATGTAGACAAGTCGAAGAAAATGTTGGAAGAAACACTCAAATGGAGGTCGACTTATAAGCCTGAGGAAATTCGTTGGCCTGAAGTTGCAGTTGAAGGCGAGACTGGAAAAGTTTATAGAGCAAATTTTCGTGACCGTCATGGGAGGACTGTTCTGATATTGAGACCAGCAATGCAGAACACAATGTCATTAGACAATAACATGCGGCACTTGGTGTATCTTATAGAGAATGCAGTCTTTAATCTTCCAGAGGGTCAGGAACAAATGGCTTGGTTGATAGACTTTACCGGGTGGACACTGAATACTTCTGTGCCTGTCAAATCAGCACGCGATACCATCAATATTTTACAGAACCACTATCCTGAGAGGCTAGCCGTGGCGTTTCTCTACAACCCTCCACGGATTTTTGAAGCATTTTGGAAGATTGTCAAGTACTTCTTGGATAACAAAACATTCCACAAAGTAAAGTTTGTTTACCCGAAGAAGAAAGACAGCGTGGAGCTTATGAAACACTATTTTGATGAGGAAAATCTCCCTACTGAGTTTGGAGGAAAAGCCATTCTAGAGTATGACCACGAGGAGTTCTCAAAATTAATGACTCAAGATGATGTGAAATCAGCTGCCTTTTGGGGCTGTGATAACAAGTTACATCATGCTAGCAATGGCCATTCTGGAGCTGAGGTAGCTCCTGAACCAGCATGCCTCACGGCGGCAGCTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

294

Amino Acids

33.98

Weight (kDa)

6.87

Isoelectric Point (pI)

50.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 89 - 238 7.7e-36 CRAL/TRIO domain
CRAL_TRIO_2 PF13716 103 - 247 5e-11 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 219
AccI GTMKAC 2 cut(s) 168, 212
AccII CGCG 2 cut(s) 154, 492
AciI CCGC 3 cut(s) 154, 367, 872
AcoI YGGCCR 1 cut(s) 826
AcsI RAATTY 2 cut(s) 231, 286
AcuI CTGAAG 2 cut(s) 118, 264
AdeI CACNNNGTG 1 cut(s) 376
AfaI GTAC 3 cut(s) 121, 143, 596
AfiI CCNNNNNNNGG 2 cut(s) 98, 871
AgsI TTSAA 2 cut(s) 257, 572
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
AluBI AGCT 6 cut(s) 73, 664, 782, 839, 848, 878
AluI AGCT 6 cut(s) 73, 664, 782, 839, 848, 878
Alw26I GTCTC 3 cut(s) 27, 257, 319
AoxI GGCC 3 cut(s) 100, 239, 826
ApeKI GCWGC 3 cut(s) 80, 782, 875
ApoI RAATTY 2 cut(s) 231, 286
AseI ATTAAT 1 cut(s) 755
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 1 cut(s) 89
AsuC2I CCSGG 1 cut(s) 451
AsuHPI GGTGA 1 cut(s) 70
AsuNHI GCTAGC 2 cut(s) 532, 818
AvaII GGWCC 1 cut(s) 89
AxyI CCTNAGG 2 cut(s) 54, 225
BalI TGGCCA 1 cut(s) 828
BauI CACGAG 2 cut(s) 32, 737
BbvCI CCTCAGC 1 cut(s) 840
BbvI GCAGC 2 cut(s) 67, 769
BccI CCATC 1 cut(s) 506
BceAI ACGGC 2 cut(s) 87, 521
BcnI CCSGG 1 cut(s) 451
BcoDI GTCTC 3 cut(s) 27, 257, 319
BfaI CTAG 4 cut(s) 533, 725, 819, 879
BfmI CTRYAG 1 cut(s) 47
BisI GCNGC 5 cut(s) 81, 368, 783, 873, 876
BlsI GCNGC 5 cut(s) 82, 369, 784, 874, 877
BmcAI AGTACT 1 cut(s) 596
Bme1390I CCNGG 1 cut(s) 451
Bme18I GGWCC 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 1 cut(s) 451
BmrI ACTGGG 1 cut(s) 22
BmsI GCATC 2 cut(s) 115, 139
BmtI GCTAGC 2 cut(s) 536, 822
BmuI ACTGGG 1 cut(s) 22
BplI GAGNNNNNCTC 2 cut(s) 731, 763
BpmI CTGGAG 1 cut(s) 855
Bpu10I CCTNAGC 1 cut(s) 840
BpuEI CTTGAG 1 cut(s) 747
BpuMI CCSGG 1 cut(s) 451
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 2 cut(s) 537, 560
BsaXI ACNNNNNCTCC 2 cut(s) 828, 858
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 871
Bse1I ACTGG 2 cut(s) 17, 271
Bse21I CCTNAGG 2 cut(s) 54, 225
Bse3DI GCAATG 2 cut(s) 339, 829
BseDI CCNNGG 2 cut(s) 537, 560
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 2 cut(s) 98, 871
BseMI GCAATG 2 cut(s) 339, 829
BseMII CTCAG 5 cut(s) 45, 216, 516, 693, 831
BseNI ACTGG 2 cut(s) 17, 271
BseRI GAGGAG 1 cut(s) 755
BseXI GCAGC 2 cut(s) 67, 769
Bsh1236I CGCG 2 cut(s) 154, 492
BshFI GGCC 3 cut(s) 102, 241, 828
BsiSI CCGG 1 cut(s) 450
BslI CCNNNNNNNGG 2 cut(s) 98, 871
BsmAI GTCTC 3 cut(s) 27, 257, 319
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 3 cut(s) 102, 241, 828
Bso31I GGTCTC 1 cut(s) 319
Bsp143I GATC 1 cut(s) 63
BspACI CCGC 3 cut(s) 154, 367, 872
BspANI GGCC 3 cut(s) 102, 241, 828
BspCNI CTCAG 5 cut(s) 46, 217, 517, 694, 832
BspFNI CGCG 2 cut(s) 154, 492
BspLI GGNNCC 1 cut(s) 91
BspOI GCTAGC 2 cut(s) 536, 822
BspTNI GGTCTC 1 cut(s) 319
BsrDI GCAATG 2 cut(s) 339, 829
BsrI ACTGG 2 cut(s) 17, 271
BssECI CCNNGG 2 cut(s) 537, 560
BssMI GATC 1 cut(s) 63
BssSI CACGAG 2 cut(s) 32, 737
Bst2BI CACGAG 2 cut(s) 32, 737
Bst4CI ACNGT 2 cut(s) 299, 313
BstC8I GCNNGC 5 cut(s) 130, 490, 534, 820, 862
BstDEI CTNAG 5 cut(s) 54, 225, 525, 702, 840
BstDSI CCRYGG 2 cut(s) 537, 560
BstF5I GGATG 1 cut(s) 154
BstFNI CGCG 2 cut(s) 154, 492
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 1 cut(s) 66
BstMAI GTCTC 3 cut(s) 27, 257, 319
BstMBI GATC 1 cut(s) 63
BstMWI GCNNNNNNNGC 1 cut(s) 845
BstNSI RCATGY 2 cut(s) 367, 864
BstSCI CCNGG 1 cut(s) 449
BstSFI CTRYAG 1 cut(s) 47
BstUI CGCG 2 cut(s) 154, 492
BstV1I GCAGC 2 cut(s) 67, 769
Bsu36I CCTNAGG 2 cut(s) 54, 225
BsuRI GGCC 3 cut(s) 102, 241, 828
BtgI CCRYGG 2 cut(s) 537, 560
BtsCI GGATG 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 458
Cac8I GCNNGC 5 cut(s) 130, 490, 534, 820, 862
CfoI GCGC 1 cut(s) 154
Cfr13I GGNCC 1 cut(s) 89
Csp6I GTAC 3 cut(s) 120, 142, 595
CviAII CATG 4 cut(s) 302, 364, 815, 861
CviQI GTAC 3 cut(s) 120, 142, 595
DdeI CTNAG 5 cut(s) 54, 225, 525, 702, 840
DpnI GATC 1 cut(s) 65
DpnII GATC 1 cut(s) 63
DraIII CACNNNGTG 1 cut(s) 376
EaeI YGGCCR 1 cut(s) 826
Eco31I GGTCTC 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 89
Eco57I CTGAAG 2 cut(s) 118, 264
Eco81I CCTNAGG 2 cut(s) 54, 225
FaeI CATG 4 cut(s) 305, 367, 818, 864
FaiI YATR 9 cut(s) 219, 279, 303, 365, 386, 668, 732, 816, 862
FalI AAGNNNNNCTT 2 cut(s) 584, 616
FatI CATG 4 cut(s) 301, 363, 814, 860
FblI GTMKAC 2 cut(s) 168, 212
Fnu4HI GCNGC 5 cut(s) 81, 368, 783, 873, 876
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 5 cut(s) 81, 368, 783, 873, 876
FspBI CTAG 4 cut(s) 533, 725, 819, 879
GlaI GCGC 1 cut(s) 153
GluI GCNGC 5 cut(s) 81, 368, 783, 873, 876
GsuI CTGGAG 1 cut(s) 855
HaeIII GGCC 3 cut(s) 102, 241, 828
HapII CCGG 1 cut(s) 450
HhaI GCGC 1 cut(s) 154
Hin1II CATG 4 cut(s) 305, 367, 818, 864
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HinfI GANTC 2 cut(s) 41, 760
HpaII CCGG 1 cut(s) 450
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 4 cut(s) 169, 213, 456, 637
Hpy188I TCNGA 1 cut(s) 318
Hpy8I GTNNAC 4 cut(s) 169, 213, 456, 637
HpyAV CCTTC 1 cut(s) 251
HpyCH4III ACNGT 2 cut(s) 299, 313
HpyCH4V TGCA 5 cut(s) 83, 112, 251, 337, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 845
HpyF3I CTNAG 5 cut(s) 54, 225, 525, 702, 840
Hsp92II CATG 4 cut(s) 305, 367, 818, 864
HspAI GCGC 1 cut(s) 152
Kzo9I GATC 1 cut(s) 63
LmnI GCTCC 3 cut(s) 661, 836, 853
Lsp1109I GCAGC 2 cut(s) 67, 769
LweI GCATC 2 cut(s) 115, 139
MaeI CTAG 4 cut(s) 533, 725, 819, 879
MaeIII GTNAC 2 cut(s) 293, 807
MalI GATC 1 cut(s) 65
MboI GATC 1 cut(s) 63
MboII GAAGA 7 cut(s) 73, 190, 203, 398, 595, 655, 658
MfeI CAATTG 1 cut(s) 84
MlsI TGGCCA 1 cut(s) 828
MluCI AATT 4 cut(s) 84, 231, 286, 752
MluNI TGGCCA 1 cut(s) 828
MlyI GAGTC 1 cut(s) 754
MmeI TCCRAC 1 cut(s) 168
Mox20I TGGCCA 1 cut(s) 828
MscI TGGCCA 1 cut(s) 828
MseI TTAA 2 cut(s) 402, 755
MslI CAYNNNNRTG 2 cut(s) 36, 202
Msp20I TGGCCA 1 cut(s) 828
MspA1I CMGCKG 1 cut(s) 782
MspI CCGG 1 cut(s) 450
MspR9I CCNGG 1 cut(s) 451
MunI CAATTG 1 cut(s) 84
Mva1269I GAATGC 1 cut(s) 397
MvnI CGCG 2 cut(s) 154, 492
MwoI GCNNNNNNNGC 1 cut(s) 845
NciI CCSGG 1 cut(s) 451
NdeII GATC 1 cut(s) 63
NheI GCTAGC 2 cut(s) 532, 818
NlaIII CATG 4 cut(s) 305, 367, 818, 864
NlaIV GGNNCC 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 293
NspI RCATGY 2 cut(s) 367, 864
PaeI GCATGC 1 cut(s) 864
PctI GAATGC 1 cut(s) 397
PfeI GAWTC 1 cut(s) 41
PkrI GCNGC 5 cut(s) 82, 369, 784, 874, 877
PleI GAGTC 1 cut(s) 754
PpsI GAGTC 1 cut(s) 754
PshBI ATTAAT 1 cut(s) 755
PsiI TTATAA 1 cut(s) 219
PspN4I GGNNCC 1 cut(s) 91
PspPI GGNCC 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 782
RsaI GTAC 3 cut(s) 121, 143, 596
RsaNI GTAC 3 cut(s) 120, 142, 595
RseI CAYNNNNRTG 2 cut(s) 36, 202
SalI GTCGAC 1 cut(s) 211
SaqAI TTAA 2 cut(s) 402, 755
SatI GCNGC 5 cut(s) 81, 368, 783, 873, 876
Sau3AI GATC 1 cut(s) 63
Sau96I GGNCC 1 cut(s) 89
ScaI AGTACT 1 cut(s) 596
SchI GAGTC 1 cut(s) 754
ScrFI CCNGG 1 cut(s) 451
SfaNI GCATC 2 cut(s) 115, 139
SfcI CTRYAG 1 cut(s) 47
SinI GGWCC 1 cut(s) 89
SmiMI CAYNNNNRTG 2 cut(s) 36, 202
SmlI CTYRAG 1 cut(s) 762
SmoI CTYRAG 1 cut(s) 762
SphI GCATGC 1 cut(s) 864
Sse9I AATT 4 cut(s) 84, 231, 286, 752
SsiI CCGC 3 cut(s) 154, 367, 872
SspI AATATT 1 cut(s) 505
SspMI CTAG 4 cut(s) 533, 725, 819, 879
StyD4I CCNGG 1 cut(s) 449
TaaI ACNGT 2 cut(s) 299, 313
TaqI TCGA 2 cut(s) 176, 212
TasI AATT 4 cut(s) 84, 231, 286, 752
TatI WGTACW 2 cut(s) 119, 594
TauI GCSGC 2 cut(s) 370, 875
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 2 cut(s) 402, 755
Tru9I TTAA 2 cut(s) 402, 755
TscAI CASTG 1 cut(s) 465
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 3 cut(s) 80, 782, 875
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 1 cut(s) 683
TspGWI ACGGA 1 cut(s) 577
TspRI CASTG 1 cut(s) 465
VpaK11BI GGWCC 1 cut(s) 89
VspI ATTAAT 1 cut(s) 755
XapI RAATTY 2 cut(s) 231, 286
XbaI TCTAGA 1 cut(s) 724
XceI RCATGY 2 cut(s) 367, 864
XmiI GTMKAC 2 cut(s) 168, 212
XspI CTAG 4 cut(s) 533, 725, 819, 879
ZrmI AGTACT 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.