MD08G1034200.v1.1

Protein of unknown function (DUF1195)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014678
Physical Location & Seq
Forward (+)
2406401 .. 2409396
2996 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1034200.v1.1.491

Sequence Viewer

Length: 567 bp
ATGAAGGACAACGATCCACCGACACTGATAACGATCCCATCATCAACGGCGATCAGGAAGGACGCCTCATCGTCGTCATCATCCTCGAAAACCAGAGTCTTTCTCGACAAAAATGGCTACAAGTTCTGGGCTTTAGCGGCGATTCTTCTCCTCGCTTTCTGGTCCATGTTCACCGGCTCCGTCACCCTCAAATGGTCCACCGGCAACTTATCCCACCTCTCCGACGACCTCGACATCCCGACCTTCGACGATCTCGACATTCTGGAGGTGGAGGAGAGAGAGAAGGTGGTGAGGCACATGTGGGATTTGTACACGCAGACCACCAGCACCAGCACGAGTCGATTGCCGCGGTTTTGGCAGGAGGCTTTCGAGGCGGCGTACGAGCACTTGACCAGCGATGTTGCCGCCGTTCGAGACGCCGCCGTTTCGGAGATTGCCAAGATGTCCATCCGCTCCATCGTTCTTGATCCTTTTCCTCTTCAATCAACGAACACAAGAGAGATGAAGAAGAGATCGAAGAAAGCCGAGGAAAGCAAGAAGGCGACTGCATTACGAAGTAGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1195

Protein Analysis

189

Amino Acids

21.14

Weight (kDa)

6.85

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1195 PF06708 21 - 157 2e-65 Protein of unknown function (DUF1195)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 453
AccII CGCG 1 cut(s) 349
AciI CCGC 7 cut(s) 137, 347, 349, 374, 405, 420, 451
AclWI GGATC 3 cut(s) 8, 28, 461
AcyI GRCGYC 2 cut(s) 63, 417
AfaI GTAC 2 cut(s) 311, 380
AfiI CCNNNNNNNGG 1 cut(s) 192
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 1 cut(s) 482
AluBI AGCT 1 cut(s) 564
AluI AGCT 1 cut(s) 564
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 1 cut(s) 408
AlwI GGATC 3 cut(s) 8, 28, 461
ApeKI GCWGC 1 cut(s) 561
AspS9I GGNCC 2 cut(s) 162, 195
AsuHPI GGTGA 3 cut(s) 163, 175, 301
AvaII GGWCC 2 cut(s) 162, 195
BauI CACGAG 1 cut(s) 334
Bbv12I GWGCWC 1 cut(s) 387
BccI CCATC 3 cut(s) 46, 455, 464
BceAI ACGGC 3 cut(s) 63, 392, 407
BcgI CGANNNNNNTGC 2 cut(s) 325, 359
BcoDI GTCTC 1 cut(s) 408
BfaI CTAG 1 cut(s) 565
BisI GCNGC 6 cut(s) 138, 347, 375, 405, 420, 562
BlsI GCNGC 6 cut(s) 139, 348, 376, 406, 421, 563
Bme18I GGWCC 2 cut(s) 162, 195
BmgT120I GGNCC 2 cut(s) 162, 195
BmiI GGNNCC 1 cut(s) 178
BplI GAGNNNNNCTC 2 cut(s) 87, 119
BpmI CTGGAG 1 cut(s) 284
BsaBI GATNNNNATC 2 cut(s) 32, 446
BsaHI GRCGYC 2 cut(s) 63, 417
BsaJI CCNNGG 2 cut(s) 347, 525
Bsc4I CCNNNNNNNGG 1 cut(s) 192
Bse118I RCCGGY 2 cut(s) 173, 200
Bse8I GATNNNNATC 2 cut(s) 32, 446
BseDI CCNNGG 2 cut(s) 347, 525
BseGI GGATG 3 cut(s) 80, 234, 447
BseJI GATNNNNATC 2 cut(s) 32, 446
BseLI CCNNNNNNNGG 1 cut(s) 192
BseRI GAGGAG 2 cut(s) 140, 287
Bsh1236I CGCG 1 cut(s) 349
BsiHKAI GWGCWC 1 cut(s) 387
BsiSI CCGG 2 cut(s) 174, 201
BsiWI CGTACG 1 cut(s) 378
BslI CCNNNNNNNGG 1 cut(s) 192
BsmAI GTCTC 1 cut(s) 408
BsmBI CGTCTC 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 387
Bsp1407I TGTACA 1 cut(s) 309
Bsp143I GATC 6 cut(s) 13, 33, 51, 250, 466, 512
BspACI CCGC 7 cut(s) 137, 347, 349, 374, 405, 420, 451
BspFNI CGCG 1 cut(s) 349
BspLI GGNNCC 1 cut(s) 178
BspPI GGATC 3 cut(s) 8, 28, 461
BsrBI CCGCTC 1 cut(s) 453
BsrFI RCCGGY 2 cut(s) 173, 200
BsrGI TGTACA 1 cut(s) 309
BssAI RCCGGY 2 cut(s) 173, 200
BssECI CCNNGG 2 cut(s) 347, 525
BssMI GATC 6 cut(s) 13, 33, 51, 250, 466, 512
BssNI GRCGYC 2 cut(s) 63, 417
BssSI CACGAG 1 cut(s) 334
Bst2BI CACGAG 1 cut(s) 334
Bst6I CTCTTC 2 cut(s) 483, 503
BstACI GRCGYC 2 cut(s) 63, 417
BstAUI TGTACA 1 cut(s) 309
BstDSI CCRYGG 1 cut(s) 347
BstF5I GGATG 3 cut(s) 80, 234, 447
BstFNI CGCG 1 cut(s) 349
BstKTI GATC 6 cut(s) 16, 36, 54, 253, 469, 515
BstMAI GTCTC 1 cut(s) 408
BstMBI GATC 6 cut(s) 13, 33, 51, 250, 466, 512
BstMWI GCNNNNNNNGC 3 cut(s) 137, 355, 371
BstNSI RCATGY 1 cut(s) 301
BstUI CGCG 1 cut(s) 349
BtgI CCRYGG 1 cut(s) 347
BtgZI GCGATG 1 cut(s) 411
BtsCI GGATG 3 cut(s) 80, 234, 447
BtsIMutI CAGTG 1 cut(s) 23
Cfr10I RCCGGY 2 cut(s) 173, 200
Cfr13I GGNCC 2 cut(s) 162, 195
Cfr42I CCGCGG 1 cut(s) 350
CseI GACGC 2 cut(s) 71, 425
Csp6I GTAC 2 cut(s) 310, 379
CviAII CATG 2 cut(s) 166, 298
CviJI RGCY 6 cut(s) 117, 131, 177, 365, 524, 564
CviKI_1 RGCY 6 cut(s) 117, 131, 177, 365, 524, 564
CviQI GTAC 2 cut(s) 310, 379
DpnI GATC 6 cut(s) 15, 35, 53, 252, 468, 514
DpnII GATC 6 cut(s) 13, 33, 51, 250, 466, 512
Eam1104I CTCTTC 2 cut(s) 483, 503
EarI CTCTTC 2 cut(s) 483, 503
Eco47I GGWCC 2 cut(s) 162, 195
Esp3I CGTCTC 1 cut(s) 408
FaeI CATG 2 cut(s) 169, 301
FaiI YATR 2 cut(s) 167, 299
FatI CATG 2 cut(s) 165, 297
Fnu4HI GCNGC 6 cut(s) 138, 347, 375, 405, 420, 562
FokI GGATG 3 cut(s) 67, 221, 434
Fsp4HI GCNGC 6 cut(s) 138, 347, 375, 405, 420, 562
FspBI CTAG 1 cut(s) 565
GluI GCNGC 6 cut(s) 138, 347, 375, 405, 420, 562
GsuI CTGGAG 1 cut(s) 284
HapII CCGG 2 cut(s) 174, 201
HgaI GACGC 2 cut(s) 71, 425
Hin1I GRCGYC 2 cut(s) 63, 417
Hin1II CATG 2 cut(s) 169, 301
HinfI GANTC 3 cut(s) 96, 142, 337
HpaII CCGG 2 cut(s) 174, 201
HphI GGTGA 3 cut(s) 163, 175, 301
Hpy166II GTNNAC 3 cut(s) 171, 198, 312
Hpy188I TCNGA 2 cut(s) 223, 430
Hpy188III TCNNGA 7 cut(s) 55, 104, 238, 254, 263, 413, 464
Hpy8I GTNNAC 3 cut(s) 171, 198, 312
Hpy99I CGWCG 3 cut(s) 76, 227, 251
HpyAV CCTTC 4 cut(s) 52, 253, 277, 532
HpyCH4V TGCA 1 cut(s) 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 355, 371
Hsp92I GRCGYC 2 cut(s) 63, 417
Hsp92II CATG 2 cut(s) 169, 301
KspI CCGCGG 1 cut(s) 350
Kzo9I GATC 6 cut(s) 13, 33, 51, 250, 466, 512
LmnI GCTCC 2 cut(s) 182, 458
MaeI CTAG 1 cut(s) 565
MaeIII GTNAC 1 cut(s) 181
MalI GATC 6 cut(s) 15, 35, 53, 252, 468, 514
MbiI CCGCTC 1 cut(s) 453
MboI GATC 6 cut(s) 13, 33, 51, 250, 466, 512
MboII GAAGA 5 cut(s) 137, 470, 517, 520, 529
MhlI GDGCHC 1 cut(s) 387
MlyI GAGTC 2 cut(s) 105, 346
MmeI TCCRAC 1 cut(s) 246
MspA1I CMGCKG 1 cut(s) 349
MspI CCGG 2 cut(s) 174, 201
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 3 cut(s) 137, 355, 371
NdeII GATC 6 cut(s) 13, 33, 51, 250, 466, 512
NlaIII CATG 2 cut(s) 169, 301
NlaIV GGNNCC 1 cut(s) 178
NmeAIII GCCGAG 1 cut(s) 550
NmuCI GTSAC 1 cut(s) 181
NspI RCATGY 1 cut(s) 301
PciI ACATGT 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PfeI GAWTC 1 cut(s) 142
Pfl23II CGTACG 1 cut(s) 378
PkrI GCNGC 6 cut(s) 139, 348, 376, 406, 421, 563
PleI GAGTC 2 cut(s) 104, 345
PpsI GAGTC 2 cut(s) 104, 345
PscI ACATGT 1 cut(s) 297
PspLI CGTACG 1 cut(s) 378
PspN4I GGNNCC 1 cut(s) 178
PspPI GGNCC 2 cut(s) 162, 195
RsaI GTAC 2 cut(s) 311, 380
RsaNI GTAC 2 cut(s) 310, 379
SacII CCGCGG 1 cut(s) 350
SatI GCNGC 6 cut(s) 138, 347, 375, 405, 420, 562
Sau3AI GATC 6 cut(s) 13, 33, 51, 250, 466, 512
Sau96I GGNCC 2 cut(s) 162, 195
SchI GAGTC 2 cut(s) 105, 346
SduI GDGCHC 1 cut(s) 387
SetI ASST 6 cut(s) 219, 231, 245, 270, 288, 566
Sfr303I CCGCGG 1 cut(s) 350
SgrBI CCGCGG 1 cut(s) 350
SinI GGWCC 2 cut(s) 162, 195
SsiI CCGC 7 cut(s) 137, 347, 349, 374, 405, 420, 451
SspMI CTAG 1 cut(s) 565
TaqI TCGA 9 cut(s) 86, 105, 231, 246, 255, 340, 369, 412, 515
TatI WGTACW 1 cut(s) 309
TauI GCSGC 5 cut(s) 140, 349, 377, 407, 422
TfiI GAWTC 1 cut(s) 142
TscAI CASTG 1 cut(s) 30
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 1 cut(s) 561
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 2 cut(s) 17, 518
TspGWI ACGGA 1 cut(s) 169
TspRI CASTG 1 cut(s) 30
VpaK11BI GGWCC 2 cut(s) 162, 195
XceI RCATGY 1 cut(s) 301
XspI CTAG 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.