Rroxscaffold_7G00167370

Protein of unknown function (DUF1195)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014678
Physical Location & Seq
Reverse (-)
8511863 .. 8513187
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00167370.1

Sequence Viewer

Length: 609 bp
ATGAAAGACGAAGATGCACCACCGACACTGGTCTCAATTCCCTCACCAACCACCGCCGCAACTCCGCTGATCAGGAAACCGGAGACCTCACCCTCGTCTTCGTCAAAAGCAGCTCGGCTGTTTGTTGTCAAGAATCGCTACAAGTTCTGGGCTCTAGCGGCGATTCTGTTGCTAGCTTTCTGGTCTATGTTCACCGGCTCCGTTACTCTCAGATGGTCTGCCGGCCACTTAACCGGCCTTTCCGACCAGCTCAACCTCCCGACCTACGACGATCTTGATATTCTGGAAGTGGAGGACAGAGAGAAGGTGGTGAGGCACTTGTGGGATTTGTACACGCAGAGGCAGACGGGGGCGAGCAGAAGAAGCACGACCCGACTGCCTCGGTTTTGGCAGGAGGCTTTTGAAGCTGCTTATGATCCTTTGAGCAGTGATGTTCCTGCTGTCAGAAACGCTGCCGTTTCTGAGATTGCTAAGATGTCCATTCGCTCCATCAATGTTGTTGACCCCTTCCCACTCCAATCCAAGGGCGTTAGGGAATTGAAAAGGAAGAAGATCAAGAAAACAAAGGAAAACAAGGAGGTAACTCATACAGTAGGGAGCAACTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1195

Protein Analysis

202

Amino Acids

22.7

Weight (kDa)

9.76

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1195 PF06708 16 - 169 9.8e-61 Protein of unknown function (DUF1195)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 54, 57, 65, 158
AclWI GGATC 1 cut(s) 410
AcoI YGGCCR 1 cut(s) 223
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 1 cut(s) 523
AgsI TTSAA 2 cut(s) 404, 541
AluBI AGCT 4 cut(s) 113, 176, 250, 407
AluI AGCT 4 cut(s) 113, 176, 250, 407
Alw26I GTCTC 2 cut(s) 37, 77
AlwI GGATC 1 cut(s) 410
AoxI GGCC 2 cut(s) 223, 235
ApeKI GCWGC 3 cut(s) 110, 407, 452
AsuHPI GGTGA 4 cut(s) 36, 81, 184, 322
AsuNHI GCTAGC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 154
BbsI GAAGAC 1 cut(s) 90
BbvI GCAGC 3 cut(s) 122, 394, 439
BccI CCATC 2 cut(s) 207, 497
BceAI ACGGC 1 cut(s) 440
BcgI CGANNNNNNTGC 4 cut(s) 151, 185, 358, 392
BclI TGATCA 1 cut(s) 69
BcoDI GTCTC 2 cut(s) 37, 77
BfaI CTAG 2 cut(s) 155, 173
BisI GCNGC 5 cut(s) 57, 111, 159, 408, 453
BlsI GCNGC 5 cut(s) 58, 112, 160, 409, 454
BmiI GGNNCC 1 cut(s) 199
BmsI GCATC 1 cut(s) 4
BmtI GCTAGC 1 cut(s) 176
BoxI GACNNNNGTC 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 90
BsaI GGTCTC 2 cut(s) 37, 77
BsaJI CCNNGG 2 cut(s) 380, 522
BsaWI WCCGGW 1 cut(s) 79
Bsc4I CCNNNNNNNGG 1 cut(s) 523
Bse118I RCCGGY 3 cut(s) 194, 221, 233
Bse1I ACTGG 1 cut(s) 33
BseDI CCNNGG 2 cut(s) 380, 522
BseLI CCNNNNNNNGG 1 cut(s) 523
BseMII CTCAG 2 cut(s) 223, 453
BseNI ACTGG 1 cut(s) 33
BseXI GCAGC 3 cut(s) 122, 394, 439
BshFI GGCC 2 cut(s) 225, 237
BsiSI CCGG 4 cut(s) 80, 195, 222, 234
BslI CCNNNNNNNGG 1 cut(s) 523
BsmAI GTCTC 2 cut(s) 37, 77
BsnI GGCC 2 cut(s) 225, 237
Bso31I GGTCTC 2 cut(s) 37, 77
Bsp1286I GDGCHC 1 cut(s) 154
Bsp1407I TGTACA 1 cut(s) 330
Bsp143I GATC 4 cut(s) 69, 271, 415, 552
BspACI CCGC 4 cut(s) 54, 57, 65, 158
BspANI GGCC 2 cut(s) 225, 237
BspCNI CTCAG 2 cut(s) 222, 454
BspHI TCATGA 1 cut(s) 605
BspLI GGNNCC 1 cut(s) 199
BspOI GCTAGC 1 cut(s) 176
BspPI GGATC 1 cut(s) 410
BspTNI GGTCTC 2 cut(s) 37, 77
BsrFI RCCGGY 3 cut(s) 194, 221, 233
BsrGI TGTACA 1 cut(s) 330
BsrI ACTGG 1 cut(s) 33
BssAI RCCGGY 3 cut(s) 194, 221, 233
BssECI CCNNGG 2 cut(s) 380, 522
BssMI GATC 4 cut(s) 69, 271, 415, 552
BssT1I CCWWGG 1 cut(s) 522
Bst4CI ACNGT 1 cut(s) 592
BstAUI TGTACA 1 cut(s) 330
BstC8I GCNNGC 3 cut(s) 174, 223, 355
BstDEI CTNAG 3 cut(s) 209, 462, 471
BstKTI GATC 4 cut(s) 72, 274, 418, 555
BstMAI GTCTC 2 cut(s) 37, 77
BstMBI GATC 4 cut(s) 69, 271, 415, 552
BstMWI GCNNNNNNNGC 3 cut(s) 158, 363, 404
BstPAI GACNNNNGTC 1 cut(s) 29
BstV1I GCAGC 3 cut(s) 122, 394, 439
BstV2I GAAGAC 1 cut(s) 90
BsuRI GGCC 2 cut(s) 225, 237
BtsI GCAGTG 1 cut(s) 433
BtsIMutI CAGTG 2 cut(s) 26, 433
Cac8I GCNNGC 3 cut(s) 174, 223, 355
CciI TCATGA 1 cut(s) 605
Cfr10I RCCGGY 3 cut(s) 194, 221, 233
Csp6I GTAC 1 cut(s) 331
CviAII CATG 1 cut(s) 606
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 3 cut(s) 209, 462, 471
DpnI GATC 4 cut(s) 71, 273, 417, 554
DpnII GATC 4 cut(s) 69, 271, 415, 552
EaeI YGGCCR 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 522
Eco24I GRGCYC 1 cut(s) 154
Eco31I GGTCTC 2 cut(s) 37, 77
EcoT14I CCWWGG 1 cut(s) 522
EcoT38I GRGCYC 1 cut(s) 154
ErhI CCWWGG 1 cut(s) 522
FaeI CATG 1 cut(s) 609
FaiI YATR 4 cut(s) 188, 414, 588, 607
FatI CATG 1 cut(s) 605
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 5 cut(s) 57, 111, 159, 408, 453
FriOI GRGCYC 1 cut(s) 154
Fsp4HI GCNGC 5 cut(s) 57, 111, 159, 408, 453
FspBI CTAG 2 cut(s) 155, 173
GluI GCNGC 5 cut(s) 57, 111, 159, 408, 453
HaeIII GGCC 2 cut(s) 225, 237
HapII CCGG 4 cut(s) 80, 195, 222, 234
Hin1II CATG 1 cut(s) 609
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HinfI GANTC 2 cut(s) 133, 163
HpaII CCGG 4 cut(s) 80, 195, 222, 234
HphI GGTGA 4 cut(s) 36, 81, 184, 322
Hpy166II GTNNAC 3 cut(s) 192, 333, 502
Hpy188I TCNGA 4 cut(s) 212, 244, 446, 463
Hpy188III TCNNGA 7 cut(s) 73, 130, 259, 275, 284, 556, 606
Hpy8I GTNNAC 3 cut(s) 192, 333, 502
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 2 cut(s) 298, 517
HpyCH4III ACNGT 1 cut(s) 592
HpyCH4V TGCA 1 cut(s) 17
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 363, 404
HpyF3I CTNAG 3 cut(s) 209, 462, 471
Hsp92II CATG 1 cut(s) 609
KroI GCCGGC 1 cut(s) 221
KroNI GCCGGC 1 cut(s) 223
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 4 cut(s) 69, 271, 415, 552
LmnI GCTCC 3 cut(s) 203, 491, 597
Lsp1109I GCAGC 3 cut(s) 122, 394, 439
LweI GCATC 1 cut(s) 4
MaeI CTAG 2 cut(s) 155, 173
MaeIII GTNAC 2 cut(s) 202, 580
MalI GATC 4 cut(s) 71, 273, 417, 554
MboI GATC 4 cut(s) 69, 271, 415, 552
MboII GAAGA 5 cut(s) 23, 90, 372, 559, 562
MhlI GDGCHC 1 cut(s) 154
MluCI AATT 2 cut(s) 36, 536
MmeI TCCRAC 1 cut(s) 267
MroNI GCCGGC 1 cut(s) 221
MseI TTAA 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 67
MspI CCGG 4 cut(s) 80, 195, 222, 234
MwoI GCNNNNNNNGC 3 cut(s) 158, 363, 404
NaeI GCCGGC 1 cut(s) 223
NdeII GATC 4 cut(s) 69, 271, 415, 552
NgoMIV GCCGGC 1 cut(s) 221
NheI GCTAGC 1 cut(s) 172
NlaIII CATG 1 cut(s) 609
NlaIV GGNNCC 1 cut(s) 199
NmeAIII GCCGAG 1 cut(s) 94
PagI TCATGA 1 cut(s) 605
PdiI GCCGGC 1 cut(s) 223
PfeI GAWTC 2 cut(s) 133, 163
PkrI GCNGC 5 cut(s) 58, 112, 160, 409, 454
PshAI GACNNNNGTC 1 cut(s) 29
PspN4I GGNNCC 1 cut(s) 199
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
SaqAI TTAA 1 cut(s) 230
SatI GCNGC 5 cut(s) 57, 111, 159, 408, 453
Sau3AI GATC 4 cut(s) 69, 271, 415, 552
SduI GDGCHC 1 cut(s) 154
SetI ASST 9 cut(s) 89, 115, 178, 252, 258, 266, 309, 409, 582
SfaNI GCATC 1 cut(s) 4
Sse9I AATT 2 cut(s) 36, 536
SsiI CCGC 4 cut(s) 54, 57, 65, 158
SspMI CTAG 2 cut(s) 155, 173
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 1 cut(s) 592
TasI AATT 2 cut(s) 36, 536
TatI WGTACW 1 cut(s) 330
TauI GCSGC 2 cut(s) 59, 161
TfiI GAWTC 2 cut(s) 133, 163
Tru1I TTAA 1 cut(s) 230
Tru9I TTAA 1 cut(s) 230
TscAI CASTG 2 cut(s) 33, 433
TseI GCWGC 3 cut(s) 110, 407, 452
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 2 cut(s) 33, 433
XspI CTAG 2 cut(s) 155, 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.