MD15G1031100.v1.1

Protein of unknown function (DUF1195)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014678
Physical Location & Seq
Forward (+)
1897088 .. 1899561
2474 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1031100.v1.1.491

Sequence Viewer

Length: 567 bp
ATGAAGGACAACGATTCACCGACACTGATAACAATCTCATCATCAACGGTGATCAGGAAGGACACCTCGTCGTCTTCGTCGTCGAAAGCCGGAGTCTTTCTCGACAAGAATCGCTACAAGTTCTCGGTTTTAGCTGCGATTCTCCTCCTCGCTTTCTGGTCAATATTCACCGGGTGCGTCACCCTCAAATGGTCAGCCGGCAACTTATCCCACGTCTCCGACGACCTCGATATACCGACCTTCGACGATCTCGACATTCTGGAGGTGGAGGAGAGAGAGACGGTGGTGAGGCACATGTGGGATTTGTACACGCAGAGCAGCCGCACCAGCACGAGTCGACTGCCGCGGTTTTGGCAGGAGGCTTTCGAGGCGGCGTTCGAGCATTTGAGCAGCGACGTTGCTGCCGTACGAGACGCCGCTGTTTCGGAGATTGCCAAGATGTCCATCCGCTCCATCGTCCTTGATCCCATCCCACTTCAATCAACGAACATAAGAGAAATGCAGAAGAAGAGATCCAAGAAAGCGGAGGAAAGCAAGAAGGTGACTACATTACGTAGTAGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1195

Protein Analysis

189

Amino Acids

21.1

Weight (kDa)

6.84

Isoelectric Point (pI)

53.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1195 PF06708 15 - 157 2.4e-61 Protein of unknown function (DUF1195)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 450
AccI GTMKAC 1 cut(s) 337
AccII CGCG 1 cut(s) 346
AciI CCGC 7 cut(s) 322, 344, 346, 371, 417, 448, 524
AclWI GGATC 2 cut(s) 458, 507
AcyI GRCGYC 1 cut(s) 414
AdeI CACNNNGTG 1 cut(s) 174
AfaI GTAC 2 cut(s) 308, 408
AfiI CCNNNNNNNGG 1 cut(s) 189
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 1 cut(s) 479
AhdI GACNNNNNGTC 1 cut(s) 67
AjiI CACGTC 1 cut(s) 214
AluBI AGCT 1 cut(s) 134
AluI AGCT 1 cut(s) 134
Alw26I GTCTC 3 cut(s) 220, 272, 405
AlwI GGATC 2 cut(s) 458, 507
ApeKI GCWGC 4 cut(s) 134, 318, 390, 401
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 6 cut(s) 9, 61, 160, 172, 298, 553
BauI CACGAG 1 cut(s) 331
BbsI GAAGAC 1 cut(s) 66
BbvI GCAGC 4 cut(s) 121, 330, 388, 402
BccI CCATC 3 cut(s) 452, 461, 476
BceAI ACGGC 1 cut(s) 389
BcgI CGANNNNNNTGC 4 cut(s) 322, 356, 383, 417
BclI TGATCA 1 cut(s) 51
BcnI CCSGG 1 cut(s) 172
BcoDI GTCTC 3 cut(s) 220, 272, 405
BisI GCNGC 8 cut(s) 135, 319, 322, 344, 372, 391, 402, 417
BlsI GCNGC 8 cut(s) 136, 320, 323, 345, 373, 392, 403, 418
Bme1390I CCNGG 1 cut(s) 172
BmeRI GACNNNNNGTC 1 cut(s) 67
BmgBI CACGTC 1 cut(s) 214
BmrFI CCNGG 1 cut(s) 172
BpiI GAAGAC 1 cut(s) 66
BplI GAGNNNNNCTC 2 cut(s) 84, 116
BpmI CTGGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 172
BsaAI YACGTR 1 cut(s) 554
BsaBI GATNNNNATC 2 cut(s) 32, 443
BsaHI GRCGYC 1 cut(s) 414
BsaJI CCNNGG 1 cut(s) 344
Bsc4I CCNNNNNNNGG 1 cut(s) 189
Bse118I RCCGGY 1 cut(s) 197
Bse8I GATNNNNATC 2 cut(s) 32, 443
BseDI CCNNGG 1 cut(s) 344
BseGI GGATG 2 cut(s) 444, 468
BseJI GATNNNNATC 2 cut(s) 32, 443
BseLI CCNNNNNNNGG 1 cut(s) 189
BseRI GAGGAG 3 cut(s) 134, 137, 284
BseXI GCAGC 4 cut(s) 121, 330, 388, 402
Bsh1236I CGCG 1 cut(s) 346
BsiSI CCGG 3 cut(s) 90, 171, 198
BsiWI CGTACG 1 cut(s) 406
BslI CCNNNNNNNGG 1 cut(s) 189
BsmAI GTCTC 3 cut(s) 220, 272, 405
BsmBI CGTCTC 3 cut(s) 220, 272, 405
Bsp1407I TGTACA 1 cut(s) 306
Bsp143I GATC 4 cut(s) 51, 247, 463, 512
BspACI CCGC 7 cut(s) 322, 344, 346, 371, 417, 448, 524
BspFNI CGCG 1 cut(s) 346
BspPI GGATC 2 cut(s) 458, 507
BsrBI CCGCTC 1 cut(s) 450
BsrFI RCCGGY 1 cut(s) 197
BsrGI TGTACA 1 cut(s) 306
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 1 cut(s) 344
BssMI GATC 4 cut(s) 51, 247, 463, 512
BssNI GRCGYC 1 cut(s) 414
BssSI CACGAG 1 cut(s) 331
Bst2BI CACGAG 1 cut(s) 331
Bst4CI ACNGT 2 cut(s) 49, 283
Bst6I CTCTTC 1 cut(s) 503
BstACI GRCGYC 1 cut(s) 414
BstAUI TGTACA 1 cut(s) 306
BstBAI YACGTR 1 cut(s) 554
BstC8I GCNNGC 1 cut(s) 199
BstDSI CCRYGG 1 cut(s) 344
BstF5I GGATG 2 cut(s) 444, 468
BstFNI CGCG 1 cut(s) 346
BstKTI GATC 4 cut(s) 54, 250, 466, 515
BstMAI GTCTC 3 cut(s) 220, 272, 405
BstMBI GATC 4 cut(s) 51, 247, 463, 512
BstMWI GCNNNNNNNGC 3 cut(s) 327, 352, 368
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 170
BstSNI TACGTA 1 cut(s) 554
BstUI CGCG 1 cut(s) 346
BstV1I GCAGC 4 cut(s) 121, 330, 388, 402
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 512
BstYI RGATCY 1 cut(s) 512
BtgI CCRYGG 1 cut(s) 344
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 2 cut(s) 444, 468
BtsIMutI CAGTG 1 cut(s) 23
Cac8I GCNNGC 1 cut(s) 199
Cfr10I RCCGGY 1 cut(s) 197
Cfr42I CCGCGG 1 cut(s) 347
CseI GACGC 2 cut(s) 166, 422
Csp6I GTAC 2 cut(s) 307, 407
CviAII CATG 1 cut(s) 295
CviJI RGCY 5 cut(s) 89, 134, 197, 321, 362
CviKI_1 RGCY 5 cut(s) 89, 134, 197, 321, 362
CviQI GTAC 2 cut(s) 307, 407
DpnI GATC 4 cut(s) 53, 249, 465, 514
DpnII GATC 4 cut(s) 51, 247, 463, 512
DraIII CACNNNGTG 1 cut(s) 174
DriI GACNNNNNGTC 1 cut(s) 67
Eam1104I CTCTTC 1 cut(s) 503
Eam1105I GACNNNNNGTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 503
Eco105I TACGTA 1 cut(s) 554
Esp3I CGTCTC 3 cut(s) 220, 272, 405
FaeI CATG 1 cut(s) 298
FaiI YATR 3 cut(s) 233, 296, 491
FatI CATG 1 cut(s) 294
FbaI TGATCA 1 cut(s) 51
FblI GTMKAC 1 cut(s) 337
Fnu4HI GCNGC 8 cut(s) 135, 319, 322, 344, 372, 391, 402, 417
FokI GGATG 2 cut(s) 431, 455
Fsp4HI GCNGC 8 cut(s) 135, 319, 322, 344, 372, 391, 402, 417
GluI GCNGC 8 cut(s) 135, 319, 322, 344, 372, 391, 402, 417
GsuI CTGGAG 1 cut(s) 281
HapII CCGG 3 cut(s) 90, 171, 198
HgaI GACGC 2 cut(s) 166, 422
Hin1I GRCGYC 1 cut(s) 414
Hin1II CATG 1 cut(s) 298
HincII GTYRAC 1 cut(s) 338
HindII GTYRAC 1 cut(s) 338
HinfI GANTC 5 cut(s) 14, 93, 109, 139, 334
HpaII CCGG 3 cut(s) 90, 171, 198
HphI GGTGA 6 cut(s) 9, 61, 160, 172, 298, 553
Hpy166II GTNNAC 2 cut(s) 309, 338
Hpy188I TCNGA 2 cut(s) 220, 427
Hpy188III TCNNGA 4 cut(s) 55, 101, 251, 260
Hpy8I GTNNAC 2 cut(s) 309, 338
Hpy99I CGWCG 6 cut(s) 73, 82, 85, 224, 248, 398
HpyAV CCTTC 3 cut(s) 52, 250, 532
HpyCH4III ACNGT 2 cut(s) 49, 283
HpyCH4IV ACGT 3 cut(s) 213, 396, 553
HpyCH4V TGCA 1 cut(s) 502
HpyF10VI GCNNNNNNNGC 3 cut(s) 327, 352, 368
HpySE526I ACGT 3 cut(s) 213, 396, 553
Hsp92I GRCGYC 1 cut(s) 414
Hsp92II CATG 1 cut(s) 298
KroI GCCGGC 1 cut(s) 197
KroNI GCCGGC 1 cut(s) 199
Ksp22I TGATCA 1 cut(s) 51
KspI CCGCGG 1 cut(s) 347
Kzo9I GATC 4 cut(s) 51, 247, 463, 512
LmnI GCTCC 1 cut(s) 455
LpnPI CCDG 8 cut(s) 40, 103, 142, 184, 211, 245, 340, 341
Lsp1109I GCAGC 4 cut(s) 121, 330, 388, 402
MaeII ACGT 3 cut(s) 213, 396, 553
MaeIII GTNAC 2 cut(s) 178, 541
MalI GATC 4 cut(s) 53, 249, 465, 514
MbiI CCGCTC 1 cut(s) 450
MboI GATC 4 cut(s) 51, 247, 463, 512
MboII GAAGA 3 cut(s) 66, 517, 520
MflI RGATCY 1 cut(s) 512
MlyI GAGTC 2 cut(s) 102, 343
MmeI TCCRAC 1 cut(s) 243
MroNI GCCGGC 1 cut(s) 197
MspA1I CMGCKG 2 cut(s) 346, 419
MspI CCGG 3 cut(s) 90, 171, 198
MspR9I CCNGG 1 cut(s) 172
MvnI CGCG 1 cut(s) 346
MwoI GCNNNNNNNGC 3 cut(s) 327, 352, 368
NaeI GCCGGC 1 cut(s) 199
NciI CCSGG 1 cut(s) 172
NdeII GATC 4 cut(s) 51, 247, 463, 512
NgoMIV GCCGGC 1 cut(s) 197
NlaIII CATG 1 cut(s) 298
NmuCI GTSAC 2 cut(s) 178, 541
NspI RCATGY 1 cut(s) 298
PciI ACATGT 1 cut(s) 294
PcsI WCGNNNNNNNCGW 5 cut(s) 74, 77, 219, 249, 402
PdiI GCCGGC 1 cut(s) 199
PfeI GAWTC 3 cut(s) 14, 109, 139
Pfl23II CGTACG 1 cut(s) 406
PkrI GCNGC 8 cut(s) 136, 320, 323, 345, 373, 392, 403, 418
PleI GAGTC 2 cut(s) 101, 342
PpsI GAGTC 2 cut(s) 101, 342
Ppu21I YACGTR 1 cut(s) 554
PscI ACATGT 1 cut(s) 294
PspLI CGTACG 1 cut(s) 406
PsuI RGATCY 1 cut(s) 512
RsaI GTAC 2 cut(s) 308, 408
RsaNI GTAC 2 cut(s) 307, 407
SacII CCGCGG 1 cut(s) 347
SalI GTCGAC 1 cut(s) 336
SatI GCNGC 8 cut(s) 135, 319, 322, 344, 372, 391, 402, 417
Sau3AI GATC 4 cut(s) 51, 247, 463, 512
SchI GAGTC 2 cut(s) 102, 343
ScrFI CCNGG 1 cut(s) 172
SetI ASST 9 cut(s) 68, 136, 216, 228, 242, 267, 399, 543, 556
Sfr303I CCGCGG 1 cut(s) 347
SgrBI CCGCGG 1 cut(s) 347
SnaBI TACGTA 1 cut(s) 554
SsiI CCGC 7 cut(s) 322, 344, 346, 371, 417, 448, 524
SspI AATATT 1 cut(s) 165
StyD4I CCNGG 1 cut(s) 170
TaaI ACNGT 2 cut(s) 49, 283
TaiI ACGT 3 cut(s) 216, 399, 556
TaqI TCGA 8 cut(s) 83, 102, 228, 243, 252, 337, 366, 378
TatI WGTACW 1 cut(s) 306
TauI GCSGC 4 cut(s) 324, 346, 374, 419
TfiI GAWTC 3 cut(s) 14, 109, 139
TscAI CASTG 1 cut(s) 30
TseFI GTSAC 2 cut(s) 178, 541
TseI GCWGC 4 cut(s) 134, 318, 390, 401
Tsp45I GTSAC 2 cut(s) 178, 541
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 30
XceI RCATGY 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.