Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002541

Physical Location & Seq

Genomic Coordinates Forward (+)

5308240 .. 5311649

Gene Structure 3410 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1066300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1659 bp

ATGGAGGATATGTCTCCGGTGGTCGCGTTGCCATTTAGAGTAGGTAACTCAGTCTGTGATAACCCAACCATAGCTGCCCACATCGATATCACAAGCCTTAAGCTAATGAAAAACACGGCGGGGTTTTTATCTGGTTCTGTCACCATGGGTTCCAGTGAGACAGTTGCAGCTGGTGAAGAGGATTGTAATTGTAGTTGCTCAGGTAATGATAATTTTGGAGCAGTTTCTGCGCCGAAAGAGGACAAGGGAGGGGGAGCTCCTTTGTTTGATATGATATCTCAAGACGAACGCAATTGGGTTTCGTCTGCTGATTTGGTAGGCCGTGAGAGTGAGGACGATGATTCTTTATCATTGGAAGGCGATCAGGTTCTTGATAGCTTTTGTTCAGTCTCAGTGGCGAGTGAGTCAAGTAGCTTGTGTTTAGAGGACTTCCTCGTCTATGAGATTGGTCCTGATGCAGGCACACTGAGTTCTGTAGACACGGAGCAGAGCATTTGCTGTGTTGATGTTGTCGATAAGGCTACAGATTTAGGTGATTCAAATTTTGAGACAGAGATCACAAGTGACCCCCTTGCTGTGACGGGGAACCCTGAGGAAGAAATTGGCGATGGGTCTGATCCAAAGCCATCTGCAGTTGTTGATGAATTACCTGTGGAAACGGGGGCCAAAGAAACAGTCAGCCGCAGTGTTTTTGAGGTAGACTATGTGCCGCTTTGGGGGTTTACATCCGTGATTGGAAGAAGACCAGAGATGGAAGATGCACTTGCCGCTATACCACAATTTTTGAAGATTCCAATTCAAATGCTGATTGGTGACCGCATACTTGATGGCTTGAGCAAGTGTTTACACCAAACTGTGCATTTCTTTGGAGTCTATGATGGTCATGGAGGCTCTCAGGTTGCAAACTATTGTCGTGATCGCGTTCATTTGGCTTTGGTTGAAGAGATAGAATCTGTTAAGGAAGGCCTAGTTAGTGTAAGTATCAAAGACAATTGCCAAGCGCAATGGACAAATGCATTTACGAATTGTTTTCTTAAGGTTGATACTGAGGTTGGAGGGAAGGCTGGTGTTGAGCCCATTGCCCCAGAAACTGTTGGTTCCACTGCTGTTGTTGCCCTTATTTGCTCGTCTCATATCATAGTAGCAAACTGTGGAGATTCGAGAGCAGTTCTGTGTCGTGGTAAAGAACCCATGGCATTGTCGGTGGATCATAAACCAAATCGGGAAGATGAATATGCAAGGATTGAAGCAGCTGGAGGCAAGGTCATACAATGGAATGGCCATCGTGTGTTTGGAGTTCTTGCTATGTCAAGGTCTATAGGCGATAGATATTTAAAACCGTGGATCATTCCAGAACCAGAAGTGATGTTTATTCCCCGGACAAAAGACGACGAATGCCTAATTTTAGCCAGTGACGGTCTATGGGACGTTATGTCAAACGAAGAAGTGTGTGACCTTGCTCGGAGAAGAATACTCCTCTGGCACAAGAAGAATGGCGCAGATGGACTTCCGCAGGAAAGAGGCGAAGGAATTGATCCCGCTGCCCAGGCAGCTGCGGAGTTTCTTTCAAACCGTGCCCTTCAAAAAGGAAGCAAGGACAACATTACTGTGATTGTGGTAGATTTGAAAGCTCAAAGGAAGTTTAAAAGCAAAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010035 GO:0010646 GO:0010648 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0022622 GO:0023051 GO:0023057 GO:0032501 GO:0032502 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0065007 GO:0071704 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 ko04931 map04016 map04075 map04931

Pfam Domains

Protein Families

PP2C

Protein Analysis

553

Amino Acids

59.44

Weight (kDa)

4.52

Isoelectric Point (pI)

41.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	281 - 535	2.9e-65	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002541)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450
malus_domestica	MD08G1066300.v1.1 MD15G1054500.v1.1
prunus_persica	Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1
pyrus_communis	pycom08g05500 pycom15g05160
rosa_chinensis	RchiOBHm_Chr6g0311361
rosa_laevigata	RLG00000010413
rosa_multiflora	Rmu_sc0008536.1_g000009
rosa_roxburghii	Rroxscaffold_7G00157690
rosa_rugosa	Rorug06G0387800 Rorug06G0387900 Rorug06G0388000 Rorug06G0388100 Rorug06G0388200
rosa_samantha	Rh6AG502500 Rh6BG513200 Rh6CG519000 Rh6DG505200
rosa_wichuraiana	Rw6G043760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	434
AccI	GTMKAC	2 cut(s)	477, 699
AccII	CGCG	2 cut(s)	26, 921
AciI	CCGC	8 cut(s)	119, 682, 710, 768, 817, 1511, 1539, 1556
AclWI	GGATC	4 cut(s)	611, 1215, 1352, 1529
AcoI	YGGCCR	1 cut(s)	1279
AcsI	RAATTY	1 cut(s)	541
AfiI	CCNNNNNNNGG	2 cut(s)	458, 716
AflII	CTTAAG	2 cut(s)	98, 1034
AgsI	TTSAA	8 cut(s)	540, 787, 800, 941, 1247, 1569, 1583, 1627
AhdI	GACNNNNNGTC	1 cut(s)	1432
AjnI	CCWGG	1 cut(s)	1545
AluBI	AGCT	9 cut(s)	74, 103, 170, 257, 378, 414, 1253, 1553, 1631
AluI	AGCT	9 cut(s)	74, 103, 170, 257, 378, 414, 1253, 1553, 1631
Alw21I	GWGCWC	1 cut(s)	259
Alw26I	GTCTC	5 cut(s)	18, 152, 394, 542, 1134
AlwI	GGATC	4 cut(s)	611, 1215, 1352, 1529
AlwNI	CAGNNNCTG	2 cut(s)	227, 1091
AoxI	GGCC	4 cut(s)	319, 663, 964, 1279
ApeKI	GCWGC	6 cut(s)	74, 167, 1250, 1541, 1550, 1553
ApoI	RAATTY	1 cut(s)	541
ArsI	GACNNNNNNTTYG	2 cut(s)	660, 692
AspLEI	GCGC	3 cut(s)	232, 1003, 1499
AspS9I	GGNCC	2 cut(s)	449, 663
AsuC2I	CCSGG	1 cut(s)	1378
AsuHPI	GGTGA	4 cut(s)	133, 185, 545, 824
AvaII	GGWCC	1 cut(s)	449
AxyI	CCTNAGG	1 cut(s)	591
BaeGI	GKGCMC	1 cut(s)	1579
BaeI	ACNNNNGTAYC	2 cut(s)	1035, 1068
BalI	TGGCCA	1 cut(s)	1281
BanII	GRGCYC	2 cut(s)	259, 1077
BbsI	GAAGAC	1 cut(s)	748
Bbv12I	GWGCWC	1 cut(s)	259
BbvI	GCAGC	6 cut(s)	61, 179, 1262, 1528, 1540, 1562
BccI	CCATC	7 cut(s)	602, 634, 745, 821, 872, 1290, 1496
BceAI	ACGGC	2 cut(s)	132, 306
BciT130I	CCWGG	1 cut(s)	1547
BcnI	CCSGG	1 cut(s)	1378
BcoDI	GTCTC	5 cut(s)	18, 152, 394, 542, 1134
BfaI	CTAG	1 cut(s)	968
BfmI	CTRYAG	4 cut(s)	474, 522, 630, 1317
BfrI	CTTAAG	2 cut(s)	98, 1034
BisI	GCNGC	9 cut(s)	75, 168, 682, 710, 768, 1251, 1542, 1551, 1554
BlsI	GCNGC	9 cut(s)	76, 169, 683, 711, 769, 1252, 1543, 1552, 1555
Bme1390I	CCNGG	2 cut(s)	1378, 1547
Bme18I	GGWCC	1 cut(s)	449
BmeRI	GACNNNNNGTC	1 cut(s)	1432
BmgT120I	GGNCC	2 cut(s)	449, 663
BmiI	GGNNCC	4 cut(s)	151, 587, 664, 1099
BmrFI	CCNGG	2 cut(s)	1378, 1547
BmsI	GCATC	2 cut(s)	445, 748
BpiI	GAAGAC	1 cut(s)	748
BpmI	CTGGAG	1 cut(s)	1275
Bpu10I	CCTNAGC	1 cut(s)	199
BpuEI	CTTGAG	2 cut(s)	264, 853
BpuMI	CCSGG	1 cut(s)	1378
Bsa29I	ATCGAT	1 cut(s)	84
BsaJI	CCNNGG	5 cut(s)	144, 1191, 1340, 1376, 1545
BsaWI	WCCGGW	1 cut(s)	16
BsaXI	ACNNNNNCTCC	3 cut(s)	26, 1248, 1278
Bsc4I	CCNNNNNNNGG	2 cut(s)	458, 716
Bse1I	ACTGG	2 cut(s)	153, 1410
Bse21I	CCTNAGG	1 cut(s)	591
Bse3DI	GCAATG	2 cut(s)	1010, 1077
BseBI	CCWGG	1 cut(s)	1547
BseCI	ATCGAT	1 cut(s)	84
BseDI	CCNNGG	5 cut(s)	144, 1191, 1340, 1376, 1545
BseGI	GGATG	1 cut(s)	725
BseLI	CCNNNNNNNGG	2 cut(s)	458, 716
BseMI	GCAATG	2 cut(s)	1010, 1077
BseMII	CTCAG	7 cut(s)	63, 213, 405, 458, 582, 908, 1038
BseNI	ACTGG	2 cut(s)	153, 1410
BseRI	GAGGAG	1 cut(s)	1466
BseSI	GKGCMC	1 cut(s)	1579
BseXI	GCAGC	6 cut(s)	61, 179, 1262, 1528, 1540, 1562
Bsh1236I	CGCG	2 cut(s)	26, 921
BshFI	GGCC	4 cut(s)	321, 665, 966, 1281
BshVI	ATCGAT	1 cut(s)	84
BsiHKAI	GWGCWC	1 cut(s)	259
BsiSI	CCGG	2 cut(s)	17, 1378
BslFI	GGGAC	1 cut(s)	1439
BslI	CCNNNNNNNGG	2 cut(s)	458, 716
BsmAI	GTCTC	5 cut(s)	18, 152, 394, 542, 1134
BsmBI	CGTCTC	1 cut(s)	1134
BsmFI	GGGAC	1 cut(s)	1439
BsmI	GAATGC	1 cut(s)	1400
BsnI	GGCC	4 cut(s)	321, 665, 966, 1281
Bsp1286I	GDGCHC	3 cut(s)	259, 1077, 1579
Bsp143I	GATC	7 cut(s)	361, 555, 616, 916, 1207, 1344, 1534
Bsp19I	CCATGG	2 cut(s)	144, 1191
BspACI	CCGC	8 cut(s)	119, 682, 710, 768, 817, 1511, 1539, 1556
BspANI	GGCC	4 cut(s)	321, 665, 966, 1281
BspCNI	CTCAG	7 cut(s)	62, 212, 404, 459, 583, 907, 1039
BspDI	ATCGAT	1 cut(s)	84
BspFNI	CGCG	2 cut(s)	26, 921
BspLI	GGNNCC	4 cut(s)	151, 587, 664, 1099
BspMAI	CTGCAG	1 cut(s)	634
BspPI	GGATC	4 cut(s)	611, 1215, 1352, 1529
BspTI	CTTAAG	2 cut(s)	98, 1034
BsrDI	GCAATG	2 cut(s)	1010, 1077
BsrI	ACTGG	2 cut(s)	153, 1410
BssECI	CCNNGG	5 cut(s)	144, 1191, 1340, 1376, 1545
BssMI	GATC	7 cut(s)	361, 555, 616, 916, 1207, 1344, 1534
BssT1I	CCWWGG	2 cut(s)	144, 1191
Bst2UI	CCWGG	1 cut(s)	1547
Bst4CI	ACNGT	9 cut(s)	163, 676, 856, 1093, 1151, 1341, 1418, 1574, 1609
Bst6I	CTCTTC	2 cut(s)	171, 936
BstAFI	CTTAAG	2 cut(s)	98, 1034
BstAPI	GCANNNNNTGC	1 cut(s)	227
BstC8I	GCNNGC	1 cut(s)	460
BstDEI	CTNAG	7 cut(s)	49, 199, 391, 467, 591, 894, 1047
BstDSI	CCRYGG	3 cut(s)	144, 1191, 1340
BstEII	GGTNACC	1 cut(s)	812
BstENI	CCTNNNNNAGG	1 cut(s)	456
BstF5I	GGATG	1 cut(s)	725
BstFNI	CGCG	2 cut(s)	26, 921
BstHHI	GCGC	3 cut(s)	232, 1003, 1499
BstKTI	GATC	7 cut(s)	364, 558, 619, 919, 1210, 1347, 1537
BstMAI	GTCTC	5 cut(s)	18, 152, 394, 542, 1134
BstMBI	GATC	7 cut(s)	361, 555, 616, 916, 1207, 1344, 1534
BstMWI	GCNNNNNNNGC	5 cut(s)	227, 767, 1112, 1547, 1550
BstNI	CCWGG	1 cut(s)	1547
BstPI	GGTNACC	1 cut(s)	812
BstSCI	CCNGG	2 cut(s)	1376, 1545
BstSFI	CTRYAG	4 cut(s)	474, 522, 630, 1317
BstSLI	GKGCMC	1 cut(s)	1579
BstUI	CGCG	2 cut(s)	26, 921
BstV1I	GCAGC	6 cut(s)	61, 179, 1262, 1528, 1540, 1562
BstV2I	GAAGAC	1 cut(s)	748
Bsu15I	ATCGAT	1 cut(s)	84
Bsu36I	CCTNAGG	1 cut(s)	591
BsuRI	GGCC	4 cut(s)	321, 665, 966, 1281
BsuTUI	ATCGAT	1 cut(s)	84
BtgI	CCRYGG	3 cut(s)	144, 1191, 1340
BtgZI	GCGATG	1 cut(s)	621
BtsCI	GGATG	1 cut(s)	725
BtsI	GCAGTG	2 cut(s)	691, 1101
BtsIMutI	CAGTG	6 cut(s)	160, 399, 464, 691, 1101, 1417
Cac8I	GCNNGC	1 cut(s)	460
CaiI	CAGNNNCTG	2 cut(s)	227, 1091
CfoI	GCGC	3 cut(s)	232, 1003, 1499
Cfr13I	GGNCC	2 cut(s)	449, 663
ClaI	ATCGAT	1 cut(s)	84
CspCI	CAANNNNNGTGG	4 cut(s)	765, 800, 1090, 1125
CviAII	CATG	4 cut(s)	145, 884, 1192, 1656
DdeI	CTNAG	7 cut(s)	49, 199, 391, 467, 591, 894, 1047
DpnI	GATC	7 cut(s)	363, 557, 618, 918, 1209, 1346, 1536
DpnII	GATC	7 cut(s)	361, 555, 616, 916, 1207, 1344, 1534
DraI	TTTAAA	2 cut(s)	1335, 1645
DrdI	GACNNNNNNGTC	1 cut(s)	434
DriI	GACNNNNNGTC	1 cut(s)	1432
DseDI	GACNNNNNNGTC	1 cut(s)	434
EaeI	YGGCCR	1 cut(s)	1279
Eam1104I	CTCTTC	2 cut(s)	171, 936
Eam1105I	GACNNNNNGTC	1 cut(s)	1432
EarI	CTCTTC	2 cut(s)	171, 936
Ecl136II	GAGCTC	1 cut(s)	257
Eco130I	CCWWGG	2 cut(s)	144, 1191
Eco147I	AGGCCT	1 cut(s)	966
Eco24I	GRGCYC	2 cut(s)	259, 1077
Eco32I	GATATC	2 cut(s)	88, 276
Eco47I	GGWCC	1 cut(s)	449
Eco53kI	GAGCTC	1 cut(s)	257
Eco81I	CCTNAGG	1 cut(s)	591
Eco91I	GGTNACC	1 cut(s)	812
EcoICRI	GAGCTC	1 cut(s)	257
EcoNI	CCTNNNNNAGG	1 cut(s)	456
EcoO65I	GGTNACC	1 cut(s)	812
EcoRII	CCWGG	1 cut(s)	1545
EcoRV	GATATC	2 cut(s)	88, 276
EcoT14I	CCWWGG	2 cut(s)	144, 1191
EcoT22I	ATGCAT	1 cut(s)	1018
EcoT38I	GRGCYC	2 cut(s)	259, 1077
ErhI	CCWWGG	2 cut(s)	144, 1191
Esp3I	CGTCTC	1 cut(s)	1134
FaeI	CATG	4 cut(s)	148, 887, 1195, 1659
FalI	AAGNNNNNCTT	2 cut(s)	747, 779
FaqI	GGGAC	1 cut(s)	1439
FatI	CATG	4 cut(s)	144, 883, 1191, 1655
FauI	CCCGC	2 cut(s)	112, 1546
FblI	GTMKAC	2 cut(s)	477, 699
Fnu4HI	GCNGC	9 cut(s)	75, 168, 682, 710, 768, 1251, 1542, 1551, 1554
FokI	GGATG	1 cut(s)	712
FriOI	GRGCYC	2 cut(s)	259, 1077
Fsp4HI	GCNGC	9 cut(s)	75, 168, 682, 710, 768, 1251, 1542, 1551, 1554
FspBI	CTAG	1 cut(s)	968
GlaI	GCGC	3 cut(s)	231, 1002, 1498
GluI	GCNGC	9 cut(s)	75, 168, 682, 710, 768, 1251, 1542, 1551, 1554
GsuI	CTGGAG	1 cut(s)	1275
HaeIII	GGCC	4 cut(s)	321, 665, 966, 1281
HapII	CCGG	2 cut(s)	17, 1378
HhaI	GCGC	3 cut(s)	232, 1003, 1499
Hin1II	CATG	4 cut(s)	148, 887, 1195, 1659
Hin6I	GCGC	3 cut(s)	230, 1001, 1497
HinP1I	GCGC	3 cut(s)	230, 1001, 1497
HinfI	GANTC	7 cut(s)	341, 404, 536, 790, 870, 950, 1157
HpaII	CCGG	2 cut(s)	17, 1378
HphI	GGTGA	4 cut(s)	133, 185, 545, 824
Hpy166II	GTNNAC	4 cut(s)	478, 700, 723, 845
Hpy188I	TCNGA	2 cut(s)	616, 1464
Hpy188III	TCNNGA	7 cut(s)	281, 371, 452, 914, 1161, 1223, 1352
Hpy8I	GTNNAC	4 cut(s)	478, 700, 723, 845
Hpy99I	CGWCG	1 cut(s)	1394
HpyAV	CCTTC	5 cut(s)	350, 956, 1054, 1520, 1589
HpyCH4III	ACNGT	9 cut(s)	163, 676, 856, 1093, 1151, 1341, 1418, 1574, 1609
HpyCH4IV	ACGT	1 cut(s)	1428
HpyCH4V	TGCA	8 cut(s)	167, 458, 632, 761, 859, 902, 1016, 1238
HpyF10VI	GCNNNNNNNGC	5 cut(s)	227, 767, 1112, 1547, 1550
HpyF3I	CTNAG	7 cut(s)	49, 199, 391, 467, 591, 894, 1047
HpySE526I	ACGT	1 cut(s)	1428
Hsp92II	CATG	4 cut(s)	148, 887, 1195, 1659
HspAI	GCGC	3 cut(s)	230, 1001, 1497
Kzo9I	GATC	7 cut(s)	361, 555, 616, 916, 1207, 1344, 1534
LmnI	GCTCC	4 cut(s)	218, 254, 262, 484
Lsp1109I	GCAGC	6 cut(s)	61, 179, 1262, 1528, 1540, 1562
LweI	GCATC	2 cut(s)	445, 748
MaeI	CTAG	1 cut(s)	968
MaeII	ACGT	1 cut(s)	1428
MaeIII	GTNAC	7 cut(s)	44, 139, 563, 577, 812, 1412, 1451
MalI	GATC	7 cut(s)	363, 557, 618, 918, 1209, 1346, 1536
MboI	GATC	7 cut(s)	361, 555, 616, 916, 1207, 1344, 1534
MfeI	CAATTG	2 cut(s)	292, 991
MhlI	GDGCHC	3 cut(s)	259, 1077, 1579
MlsI	TGGCCA	1 cut(s)	1281
MluNI	TGGCCA	1 cut(s)	1281
MlyI	GAGTC	2 cut(s)	413, 879
MmeI	TCCRAC	1 cut(s)	1033
Mox20I	TGGCCA	1 cut(s)	1281
Mph1103I	ATGCAT	1 cut(s)	1018
MscI	TGGCCA	1 cut(s)	1281
MseI	TTAA	5 cut(s)	99, 957, 1035, 1334, 1644
MslI	CAYNNNNRTG	1 cut(s)	1607
Msp20I	TGGCCA	1 cut(s)	1281
MspA1I	CMGCKG	4 cut(s)	170, 1253, 1541, 1553
MspCI	CTTAAG	2 cut(s)	98, 1034
MspI	CCGG	2 cut(s)	17, 1378
MspR9I	CCNGG	2 cut(s)	1378, 1547
MunI	CAATTG	2 cut(s)	292, 991
Mva1269I	GAATGC	1 cut(s)	1400
MvaI	CCWGG	1 cut(s)	1547
MvnI	CGCG	2 cut(s)	26, 921
MwoI	GCNNNNNNNGC	5 cut(s)	227, 767, 1112, 1547, 1550
NciI	CCSGG	1 cut(s)	1378
NcoI	CCATGG	2 cut(s)	144, 1191
NdeII	GATC	7 cut(s)	361, 555, 616, 916, 1207, 1344, 1534
NlaIII	CATG	4 cut(s)	148, 887, 1195, 1659
NlaIV	GGNNCC	4 cut(s)	151, 587, 664, 1099
NmuCI	GTSAC	6 cut(s)	139, 563, 577, 812, 1412, 1451
NsiI	ATGCAT	1 cut(s)	1018
PceI	AGGCCT	1 cut(s)	966
PctI	GAATGC	1 cut(s)	1400
PfeI	GAWTC	5 cut(s)	341, 536, 790, 950, 1157
PkrI	GCNGC	9 cut(s)	76, 169, 683, 711, 769, 1252, 1543, 1552, 1555
PleI	GAGTC	2 cut(s)	412, 878
PpsI	GAGTC	2 cut(s)	412, 878
Psp124BI	GAGCTC	1 cut(s)	259
Psp6I	CCWGG	1 cut(s)	1545
PspEI	GGTNACC	1 cut(s)	812
PspGI	CCWGG	1 cut(s)	1545
PspN4I	GGNNCC	4 cut(s)	151, 587, 664, 1099
PspPI	GGNCC	2 cut(s)	449, 663
PstI	CTGCAG	1 cut(s)	634
PstNI	CAGNNNCTG	2 cut(s)	227, 1091
PvuII	CAGCTG	3 cut(s)	170, 1253, 1553
RseI	CAYNNNNRTG	1 cut(s)	1607
SacI	GAGCTC	1 cut(s)	259
SaqAI	TTAA	5 cut(s)	99, 957, 1035, 1334, 1644
SatI	GCNGC	9 cut(s)	75, 168, 682, 710, 768, 1251, 1542, 1551, 1554
Sau3AI	GATC	7 cut(s)	361, 555, 616, 916, 1207, 1344, 1534
Sau96I	GGNCC	2 cut(s)	449, 663
SchI	GAGTC	2 cut(s)	413, 879
ScrFI	CCNGG	2 cut(s)	1378, 1547
SduI	GDGCHC	3 cut(s)	259, 1077, 1579
SfaNI	GCATC	2 cut(s)	445, 748
SfcI	CTRYAG	4 cut(s)	474, 522, 630, 1317
SinI	GGWCC	1 cut(s)	449
SmiMI	CAYNNNNRTG	1 cut(s)	1607
SmlI	CTYRAG	4 cut(s)	98, 279, 832, 1034
SmoI	CTYRAG	4 cut(s)	98, 279, 832, 1034
SseBI	AGGCCT	1 cut(s)	966
SsiI	CCGC	8 cut(s)	119, 682, 710, 768, 817, 1511, 1539, 1556
SspMI	CTAG	1 cut(s)	968
SstI	GAGCTC	1 cut(s)	259
StuI	AGGCCT	1 cut(s)	966
StyD4I	CCNGG	2 cut(s)	1376, 1545
StyI	CCWWGG	2 cut(s)	144, 1191
TaaI	ACNGT	9 cut(s)	163, 676, 856, 1093, 1151, 1341, 1418, 1574, 1609
TaiI	ACGT	1 cut(s)	1431
TaqI	TCGA	3 cut(s)	84, 513, 1160
TauI	GCSGC	3 cut(s)	684, 712, 770
TfiI	GAWTC	5 cut(s)	341, 536, 790, 950, 1157
Tru1I	TTAA	5 cut(s)	99, 957, 1035, 1334, 1644
Tru9I	TTAA	5 cut(s)	99, 957, 1035, 1334, 1644
TscAI	CASTG	6 cut(s)	160, 399, 471, 691, 1108, 1417
TseFI	GTSAC	6 cut(s)	139, 563, 577, 812, 1412, 1451
TseI	GCWGC	6 cut(s)	74, 167, 1250, 1541, 1550, 1553
Tsp45I	GTSAC	6 cut(s)	139, 563, 577, 812, 1412, 1451
TspDTI	ATGAA	4 cut(s)	122, 657, 914, 1245
TspGWI	ACGGA	2 cut(s)	497, 718
TspRI	CASTG	6 cut(s)	160, 399, 471, 691, 1108, 1417
Vha464I	CTTAAG	2 cut(s)	98, 1034
VpaK11BI	GGWCC	1 cut(s)	449
XagI	CCTNNNNNAGG	1 cut(s)	456
XapI	RAATTY	1 cut(s)	541
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1289
XmiI	GTMKAC	2 cut(s)	477, 699
XspI	CTAG	1 cut(s)	968
Zsp2I	ATGCAT	1 cut(s)	1018

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.