Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002541

Physical Location & Seq

Genomic Coordinates Reverse (-)

3714347 .. 3717523

Gene Structure 3177 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1054500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1659 bp

ATGGAGGATATGTCTACGCCGGTCGCGGTGCCATTTAGAGTAGGTAACTCAGTCTGTGATAACCCAACCATAGCTGTCCACATGGATATCACAAGACTTAAGCTAATGACAAACACGGCGGGATTGCTATCTGATTCTGTCACCATGGGTTCCAGTGAGACGGTTGCAGCTGGTGAAGAGGATTGTAATTTTAGTTGTTCGGGGAATGATAGTTTTGGAGTAGTTTCAGCGCCAAAAGAGGACAAGGGGGGAGGAGCTCCTTTGTTGGATATGATATCTCAAGATGAAAGCATTTGGGTTTCTTCTGCTGATGCGATAGGCCGTGAGAGTGAGGACGATGATTCTTTATCATTGGAAGGTGATCAGGTTCTTGATAGCTCTTGTTCACTCTCAGTGGCGAGTGAGTCAAGTAGCTTGTGTTTAGAGGACTTCCTCGTTTATGAGGTTGGTCCTGATGCAGGCACATTGAGTTCTGCAGACAAGGAGAAGAGCATTTGCTGTGTAGAAGTTGTCGATAAGGCCACGGATTTAGGTGATTTAAATGTTGAGACAGAGATCACAAGTGATCCCCTTCCTGTGACGGGGAGCCTTGAGGAAGTAATTGGTGATGGGTCTGACCCAAAGCCATCTGCAGTTGTTGATCAATTACCTGTGGAAACGGGGGCCAAAGAAACAGTTAGCCGCAGTGTTTTTGAGGTAGACTATGTGCCGCTTTGGGGGTTTACGTCTGTGATTGGAAGAAGGCCAGAGATGGAAGATGCGCTTGCCGCTATACCGCAACTGTTTAAGATTCCAATTCAAATGCTGATTGGTGACCGCATAATTGATGGCTTGAGCAAGTGTTTGCACCAAACTGTTCATTTCTTTGGAGTCTATGATGGTCATGGAGGGTCTCAGGTTGCAAACTATTGTCGTGACCGCATCCATTTGGCTTTAGCTGAAGAGATAGAATCTGTTAAGGAAGGCCTAATTCATGAAAGTATCAAAGACAATTGCCAAGGGCAATGGACAAAGGCATTTACAAATTGTTTTTTTAAGGTTGACACTGAGGTTGGAGGGAAGGCTGGTCTTGAGCCTGTTGCCCCAGAAACTGTTGGTTCCACTGCTGTCGTTGCCCTTATTTGCTCGTCTCATATCATAGTAGCAAACTGTGGAGATTCCAGAGCAGTTCTCTGTCGGGGTAAAGAACCAATGGCGTTATCGGTGGATCATAAACCAGATCGGGAAGACGAATATGCAAGGATTGAAGCAGCTGGAGGCAAGGTCATACAATGGAATGGCCATCGTGTTTTTGGCGTTCTTGCTATGTCAAGGTCTATAGGCGATAGATATTTAAAGCCATGCATCATTCCGGAACCAGAAGTGATGTTTATTCCCCGAACAAAAGACGATGAATGCCTCGTATTAGCGAGTGATGGTTTATGGGACGTTATGTCAAATGAAGAAGTGTGTGACCTTGCTCGGAGACGAATACTCCTCTGGCACAAGAAGAATGGTGCTGATGGACTTCCACTGGAAAGAGGCGAAGGAATTGATCCCGCTGCCCAGGCAGCTGCGGAGTTTCTTTCGAACCGTGCTCTTCAAAAAGGAAGTAAGGACAACATAACTGTGATTGTGGTGGATCTGAAAGCTCAAAGGAAGTTTAAAAGCAAAACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010035 GO:0010646 GO:0010648 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0022622 GO:0023051 GO:0023057 GO:0032501 GO:0032502 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0065007 GO:0071704 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 ko04931 map04016 map04075 map04931

Pfam Domains

Protein Families

PP2C

Protein Analysis

553

Amino Acids

59.38

Weight (kDa)

4.57

Isoelectric Point (pI)

39.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	282 - 535	4.8e-65	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002541)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450 FvH4_2g31450
malus_domestica	MD08G1066300.v1.1 MD15G1054500.v1.1
prunus_persica	Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1 Prupe.1G408100_v2.0.a1
pyrus_communis	pycom08g05500 pycom15g05160
rosa_chinensis	RchiOBHm_Chr6g0311361
rosa_laevigata	RLG00000010413
rosa_multiflora	Rmu_sc0008536.1_g000009
rosa_roxburghii	Rroxscaffold_7G00157690
rosa_rugosa	Rorug06G0387800 Rorug06G0387900 Rorug06G0388000 Rorug06G0388100 Rorug06G0388200
rosa_samantha	Rh6AG502500 Rh6BG513200 Rh6CG519000 Rh6DG505200
rosa_wichuraiana	Rw6G043760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	28
AccI	GTMKAC	2 cut(s)	14, 699
AccII	CGCG	1 cut(s)	26
AccIII	TCCGGA	1 cut(s)	1351
AclWI	GGATC	4 cut(s)	560, 1215, 1529, 1629
AcoI	YGGCCR	1 cut(s)	1279
AcuI	CTGAAG	1 cut(s)	960
AfiI	CCNNNNNNNGG	3 cut(s)	458, 581, 716
AflII	CTTAAG	1 cut(s)	98
AgsI	TTSAA	3 cut(s)	800, 1247, 1583
AhdI	GACNNNNNGTC	1 cut(s)	1432
AjnI	CCWGG	1 cut(s)	1545
Alw21I	GWGCWC	2 cut(s)	259, 1579
Alw26I	GTCTC	5 cut(s)	152, 542, 897, 1134, 1459
AlwI	GGATC	4 cut(s)	560, 1215, 1529, 1629
AlwNI	CAGNNNCTG	1 cut(s)	1091
Aor13HI	TCCGGA	1 cut(s)	1351
AoxI	GGCC	6 cut(s)	319, 519, 663, 743, 964, 1279
ApeKI	GCWGC	5 cut(s)	167, 1250, 1541, 1550, 1553
AspLEI	GCGC	2 cut(s)	232, 763
AspS9I	GGNCC	2 cut(s)	449, 663
AsuHPI	GGTGA	6 cut(s)	133, 185, 371, 545, 617, 824
AsuII	TTCGAA	1 cut(s)	1568
AvaII	GGWCC	1 cut(s)	449
BalI	TGGCCA	1 cut(s)	1281
BanI	GGYRCC	1 cut(s)	28
BanII	GRGCYC	1 cut(s)	259
BbsI	GAAGAC	1 cut(s)	1233
Bbv12I	GWGCWC	2 cut(s)	259, 1579
BbvI	GCAGC	5 cut(s)	179, 1262, 1528, 1540, 1562
BccI	CCATC	8 cut(s)	602, 634, 745, 821, 872, 1290, 1409, 1496
BceAI	ACGGC	2 cut(s)	132, 306
BciT130I	CCWGG	1 cut(s)	1547
BclI	TGATCA	2 cut(s)	361, 640
BcoDI	GTCTC	5 cut(s)	152, 542, 897, 1134, 1459
BfmI	CTRYAG	3 cut(s)	474, 630, 1317
BfoI	RGCGCY	1 cut(s)	233
BfrI	CTTAAG	1 cut(s)	98
BisI	GCNGC	8 cut(s)	168, 682, 710, 768, 1251, 1542, 1551, 1554
BlsI	GCNGC	8 cut(s)	169, 683, 711, 769, 1252, 1543, 1552, 1555
Bme1390I	CCNGG	1 cut(s)	1547
Bme18I	GGWCC	1 cut(s)	449
BmeRI	GACNNNNNGTC	1 cut(s)	1432
BmgT120I	GGNCC	2 cut(s)	449, 663
BmiI	GGNNCC	6 cut(s)	30, 151, 587, 664, 1099, 1356
BmrFI	CCNGG	1 cut(s)	1547
BmsI	GCATC	5 cut(s)	301, 445, 748, 930, 1353
BpiI	GAAGAC	1 cut(s)	1233
BplI	GAGNNNNNCTC	2 cut(s)	1155, 1187
BpmI	CTGGAG	1 cut(s)	1275
Bpu14I	TTCGAA	1 cut(s)	1568
BpuEI	CTTGAG	4 cut(s)	264, 611, 853, 1091
BsaBI	GATNNNNATC	1 cut(s)	127
BsaI	GGTCTC	1 cut(s)	897
BsaJI	CCNNGG	4 cut(s)	144, 522, 997, 1545
BsaWI	WCCGGW	1 cut(s)	1351
BsaXI	ACNNNNNCTCC	5 cut(s)	26, 1248, 1278, 1458, 1488
Bsc4I	CCNNNNNNNGG	3 cut(s)	458, 581, 716
Bse118I	RCCGGY	1 cut(s)	19
Bse1I	ACTGG	2 cut(s)	153, 1518
Bse3DI	GCAATG	1 cut(s)	1010
Bse8I	GATNNNNATC	1 cut(s)	127
BseAI	TCCGGA	1 cut(s)	1351
BseBI	CCWGG	1 cut(s)	1547
BseDI	CCNNGG	4 cut(s)	144, 522, 997, 1545
BseGI	GGATG	1 cut(s)	921
BseJI	GATNNNNATC	1 cut(s)	127
BseLI	CCNNNNNNNGG	3 cut(s)	458, 581, 716
BseMI	GCAATG	1 cut(s)	1010
BseMII	CTCAG	4 cut(s)	63, 405, 908, 1038
BseNI	ACTGG	2 cut(s)	153, 1518
BseRI	GAGGAG	2 cut(s)	267, 1466
BseXI	GCAGC	5 cut(s)	179, 1262, 1528, 1540, 1562
Bsh1236I	CGCG	1 cut(s)	26
Bsh1285I	CGRYCG	1 cut(s)	24
BshFI	GGCC	6 cut(s)	321, 521, 665, 745, 966, 1281
BshNI	GGYRCC	1 cut(s)	28
BsiEI	CGRYCG	1 cut(s)	24
BsiHKAI	GWGCWC	2 cut(s)	259, 1579
BsiSI	CCGG	2 cut(s)	20, 1352
BslFI	GGGAC	1 cut(s)	1439
BslI	CCNNNNNNNGG	3 cut(s)	458, 581, 716
BsmAI	GTCTC	5 cut(s)	152, 542, 897, 1134, 1459
BsmBI	CGTCTC	3 cut(s)	152, 1134, 1459
BsmFI	GGGAC	1 cut(s)	1439
BsmI	GAATGC	1 cut(s)	1400
BsnI	GGCC	6 cut(s)	321, 521, 665, 745, 966, 1281
Bso31I	GGTCTC	1 cut(s)	897
Bsp119I	TTCGAA	1 cut(s)	1568
Bsp1286I	GDGCHC	2 cut(s)	259, 1579
Bsp13I	TCCGGA	1 cut(s)	1351
Bsp143I	GATC	8 cut(s)	361, 555, 565, 640, 1207, 1219, 1534, 1621
Bsp19I	CCATGG	1 cut(s)	144
BspANI	GGCC	6 cut(s)	321, 521, 665, 745, 966, 1281
BspCNI	CTCAG	4 cut(s)	62, 404, 907, 1039
BspEI	TCCGGA	1 cut(s)	1351
BspFNI	CGCG	1 cut(s)	26
BspHI	TCATGA	1 cut(s)	973
BspLI	GGNNCC	6 cut(s)	30, 151, 587, 664, 1099, 1356
BspMAI	CTGCAG	2 cut(s)	478, 634
BspPI	GGATC	4 cut(s)	560, 1215, 1529, 1629
BspQI	GCTCTTC	2 cut(s)	482, 1584
BspT104I	TTCGAA	1 cut(s)	1568
BspT107I	GGYRCC	1 cut(s)	28
BspTI	CTTAAG	1 cut(s)	98
BspTNI	GGTCTC	1 cut(s)	897
BsrDI	GCAATG	1 cut(s)	1010
BsrFI	RCCGGY	1 cut(s)	19
BsrI	ACTGG	2 cut(s)	153, 1518
BssAI	RCCGGY	1 cut(s)	19
BssECI	CCNNGG	4 cut(s)	144, 522, 997, 1545
BssMI	GATC	8 cut(s)	361, 555, 565, 640, 1207, 1219, 1534, 1621
BssT1I	CCWWGG	2 cut(s)	144, 997
Bst2UI	CCWGG	1 cut(s)	1547
Bst4CI	ACNGT	8 cut(s)	163, 676, 783, 856, 1093, 1151, 1574, 1609
Bst6I	CTCTTC	4 cut(s)	171, 482, 936, 1584
BstAFI	CTTAAG	1 cut(s)	98
BstBI	TTCGAA	1 cut(s)	1568
BstC8I	GCNNGC	2 cut(s)	460, 765
BstDEI	CTNAG	4 cut(s)	49, 391, 894, 1047
BstDSI	CCRYGG	2 cut(s)	144, 522
BstEII	GGTNACC	1 cut(s)	812
BstENI	CCTNNNNNAGG	1 cut(s)	456
BstF5I	GGATG	1 cut(s)	921
BstFNI	CGCG	1 cut(s)	26
BstH2I	RGCGCY	1 cut(s)	233
BstHHI	GCGC	2 cut(s)	232, 763
BstKTI	GATC	8 cut(s)	364, 558, 568, 643, 1210, 1222, 1537, 1624
BstMAI	GTCTC	5 cut(s)	152, 542, 897, 1134, 1459
BstMBI	GATC	8 cut(s)	361, 555, 565, 640, 1207, 1219, 1534, 1621
BstMCI	CGRYCG	1 cut(s)	24
BstMWI	GCNNNNNNNGC	4 cut(s)	767, 1112, 1547, 1550
BstNI	CCWGG	1 cut(s)	1547
BstPI	GGTNACC	1 cut(s)	812
BstSCI	CCNGG	1 cut(s)	1545
BstSFI	CTRYAG	3 cut(s)	474, 630, 1317
BstUI	CGCG	1 cut(s)	26
BstV1I	GCAGC	5 cut(s)	179, 1262, 1528, 1540, 1562
BstV2I	GAAGAC	1 cut(s)	1233
BstX2I	RGATCY	1 cut(s)	1621
BstYI	RGATCY	1 cut(s)	1621
BsuRI	GGCC	6 cut(s)	321, 521, 665, 745, 966, 1281
BtgI	CCRYGG	2 cut(s)	144, 522
BtsCI	GGATG	1 cut(s)	921
BtsI	GCAGTG	2 cut(s)	691, 1101
BtsIMutI	CAGTG	6 cut(s)	160, 399, 691, 1044, 1101, 1511
Cac8I	GCNNGC	2 cut(s)	460, 765
CaiI	CAGNNNCTG	1 cut(s)	1091
CciI	TCATGA	1 cut(s)	973
CfoI	GCGC	2 cut(s)	232, 763
Cfr10I	RCCGGY	1 cut(s)	19
Cfr13I	GGNCC	2 cut(s)	449, 663
CviAII	CATG	5 cut(s)	82, 145, 884, 974, 1341
DdeI	CTNAG	4 cut(s)	49, 391, 894, 1047
DpnI	GATC	8 cut(s)	363, 557, 567, 642, 1209, 1221, 1536, 1623
DpnII	GATC	8 cut(s)	361, 555, 565, 640, 1207, 1219, 1534, 1621
DraI	TTTAAA	3 cut(s)	540, 1335, 1645
DriI	GACNNNNNGTC	1 cut(s)	1432
EaeI	YGGCCR	1 cut(s)	1279
Eam1104I	CTCTTC	4 cut(s)	171, 482, 936, 1584
Eam1105I	GACNNNNNGTC	1 cut(s)	1432
EarI	CTCTTC	4 cut(s)	171, 482, 936, 1584
Ecl136II	GAGCTC	1 cut(s)	257
Eco130I	CCWWGG	2 cut(s)	144, 997
Eco147I	AGGCCT	1 cut(s)	966
Eco24I	GRGCYC	1 cut(s)	259
Eco31I	GGTCTC	1 cut(s)	897
Eco32I	GATATC	2 cut(s)	88, 276
Eco47I	GGWCC	1 cut(s)	449
Eco53kI	GAGCTC	1 cut(s)	257
Eco57I	CTGAAG	1 cut(s)	960
Eco91I	GGTNACC	1 cut(s)	812
EcoICRI	GAGCTC	1 cut(s)	257
EcoNI	CCTNNNNNAGG	1 cut(s)	456
EcoO65I	GGTNACC	1 cut(s)	812
EcoRII	CCWGG	1 cut(s)	1545
EcoRV	GATATC	2 cut(s)	88, 276
EcoT14I	CCWWGG	2 cut(s)	144, 997
EcoT22I	ATGCAT	1 cut(s)	1346
EcoT38I	GRGCYC	1 cut(s)	259
ErhI	CCWWGG	2 cut(s)	144, 997
Esp3I	CGTCTC	3 cut(s)	152, 1134, 1459
FaeI	CATG	5 cut(s)	85, 148, 887, 977, 1344
FalI	AAGNNNNNCTT	2 cut(s)	747, 779
FaqI	GGGAC	1 cut(s)	1439
FatI	CATG	5 cut(s)	81, 144, 883, 973, 1340
FauI	CCCGC	2 cut(s)	112, 1546
FbaI	TGATCA	2 cut(s)	361, 640
FblI	GTMKAC	2 cut(s)	14, 699
Fnu4HI	GCNGC	8 cut(s)	168, 682, 710, 768, 1251, 1542, 1551, 1554
FokI	GGATG	1 cut(s)	908
FriOI	GRGCYC	1 cut(s)	259
Fsp4HI	GCNGC	8 cut(s)	168, 682, 710, 768, 1251, 1542, 1551, 1554
GlaI	GCGC	2 cut(s)	231, 762
GluI	GCNGC	8 cut(s)	168, 682, 710, 768, 1251, 1542, 1551, 1554
GsuI	CTGGAG	1 cut(s)	1275
HaeII	RGCGCY	1 cut(s)	233
HaeIII	GGCC	6 cut(s)	321, 521, 665, 745, 966, 1281
HapII	CCGG	2 cut(s)	20, 1352
HhaI	GCGC	2 cut(s)	232, 763
Hin1II	CATG	5 cut(s)	85, 148, 887, 977, 1344
Hin6I	GCGC	2 cut(s)	230, 761
HinP1I	GCGC	2 cut(s)	230, 761
HincII	GTYRAC	1 cut(s)	1042
HindII	GTYRAC	1 cut(s)	1042
HinfI	GANTC	7 cut(s)	134, 341, 404, 790, 870, 950, 1157
HpaII	CCGG	2 cut(s)	20, 1352
HphI	GGTGA	6 cut(s)	133, 185, 371, 545, 617, 824
Hpy166II	GTNNAC	6 cut(s)	15, 79, 386, 700, 723, 1042
Hpy188I	TCNGA	4 cut(s)	133, 616, 1464, 1626
Hpy188III	TCNNGA	9 cut(s)	281, 371, 452, 914, 974, 1070, 1161, 1223, 1352
Hpy8I	GTNNAC	6 cut(s)	15, 79, 386, 700, 723, 1042
HpyAV	CCTTC	6 cut(s)	350, 581, 735, 956, 1054, 1520
HpyCH4III	ACNGT	8 cut(s)	163, 676, 783, 856, 1093, 1151, 1574, 1609
HpyCH4IV	ACGT	3 cut(s)	725, 1428, 1655
HpyCH4V	TGCA	8 cut(s)	167, 458, 476, 632, 847, 902, 1238, 1344
HpyF10VI	GCNNNNNNNGC	4 cut(s)	767, 1112, 1547, 1550
HpyF3I	CTNAG	4 cut(s)	49, 391, 894, 1047
HpySE526I	ACGT	3 cut(s)	725, 1428, 1655
Hsp92II	CATG	5 cut(s)	85, 148, 887, 977, 1344
HspAI	GCGC	2 cut(s)	230, 761
Kpn2I	TCCGGA	1 cut(s)	1351
Ksp22I	TGATCA	2 cut(s)	361, 640
Kzo9I	GATC	8 cut(s)	361, 555, 565, 640, 1207, 1219, 1534, 1621
LguI	GCTCTTC	2 cut(s)	482, 1584
LmnI	GCTCC	3 cut(s)	254, 262, 585
Lsp1109I	GCAGC	5 cut(s)	179, 1262, 1528, 1540, 1562
LweI	GCATC	5 cut(s)	301, 445, 748, 930, 1353
MaeII	ACGT	3 cut(s)	725, 1428, 1655
MaeIII	GTNAC	6 cut(s)	44, 139, 577, 812, 914, 1451
MalI	GATC	8 cut(s)	363, 557, 567, 642, 1209, 1221, 1536, 1623
MboI	GATC	8 cut(s)	361, 555, 565, 640, 1207, 1219, 1534, 1621
MfeI	CAATTG	1 cut(s)	991
MflI	RGATCY	1 cut(s)	1621
MhlI	GDGCHC	2 cut(s)	259, 1579
MlsI	TGGCCA	1 cut(s)	1281
MluCI	AATT	9 cut(s)	187, 600, 644, 795, 822, 969, 991, 1024, 1530
MluNI	TGGCCA	1 cut(s)	1281
MlyI	GAGTC	2 cut(s)	413, 879
MmeI	TCCRAC	2 cut(s)	246, 1033
Mox20I	TGGCCA	1 cut(s)	1281
Mph1103I	ATGCAT	1 cut(s)	1346
MroI	TCCGGA	1 cut(s)	1351
MscI	TGGCCA	1 cut(s)	1281
MseI	TTAA	7 cut(s)	99, 539, 786, 957, 1035, 1334, 1644
MslI	CAYNNNNRTG	1 cut(s)	1607
Msp20I	TGGCCA	1 cut(s)	1281
MspA1I	CMGCKG	4 cut(s)	170, 1253, 1541, 1553
MspCI	CTTAAG	1 cut(s)	98
MspI	CCGG	2 cut(s)	20, 1352
MspR9I	CCNGG	1 cut(s)	1547
MunI	CAATTG	1 cut(s)	991
Mva1269I	GAATGC	1 cut(s)	1400
MvaI	CCWGG	1 cut(s)	1547
MvnI	CGCG	1 cut(s)	26
MwoI	GCNNNNNNNGC	4 cut(s)	767, 1112, 1547, 1550
NcoI	CCATGG	1 cut(s)	144
NdeII	GATC	8 cut(s)	361, 555, 565, 640, 1207, 1219, 1534, 1621
NlaIII	CATG	5 cut(s)	85, 148, 887, 977, 1344
NlaIV	GGNNCC	6 cut(s)	30, 151, 587, 664, 1099, 1356
NmuCI	GTSAC	5 cut(s)	139, 577, 812, 914, 1451
NsiI	ATGCAT	1 cut(s)	1346
NspV	TTCGAA	1 cut(s)	1568
PagI	TCATGA	1 cut(s)	973
PceI	AGGCCT	1 cut(s)	966
PciSI	GCTCTTC	2 cut(s)	482, 1584
PctI	GAATGC	1 cut(s)	1400
PfeI	GAWTC	5 cut(s)	134, 341, 790, 950, 1157
PkrI	GCNGC	8 cut(s)	169, 683, 711, 769, 1252, 1543, 1552, 1555
PleI	GAGTC	2 cut(s)	412, 878
PpsI	GAGTC	2 cut(s)	412, 878
Psp124BI	GAGCTC	1 cut(s)	259
Psp6I	CCWGG	1 cut(s)	1545
PspEI	GGTNACC	1 cut(s)	812
PspGI	CCWGG	1 cut(s)	1545
PspN4I	GGNNCC	6 cut(s)	30, 151, 587, 664, 1099, 1356
PspPI	GGNCC	2 cut(s)	449, 663
PstI	CTGCAG	2 cut(s)	478, 634
PstNI	CAGNNNCTG	1 cut(s)	1091
PsuI	RGATCY	1 cut(s)	1621
PvuII	CAGCTG	3 cut(s)	170, 1253, 1553
RseI	CAYNNNNRTG	1 cut(s)	1607
SacI	GAGCTC	1 cut(s)	259
SapI	GCTCTTC	2 cut(s)	482, 1584
SaqAI	TTAA	7 cut(s)	99, 539, 786, 957, 1035, 1334, 1644
SatI	GCNGC	8 cut(s)	168, 682, 710, 768, 1251, 1542, 1551, 1554
Sau3AI	GATC	8 cut(s)	361, 555, 565, 640, 1207, 1219, 1534, 1621
Sau96I	GGNCC	2 cut(s)	449, 663
SchI	GAGTC	2 cut(s)	413, 879
ScrFI	CCNGG	1 cut(s)	1547
SduI	GDGCHC	2 cut(s)	259, 1579
SfaNI	GCATC	5 cut(s)	301, 445, 748, 930, 1353
SfcI	CTRYAG	3 cut(s)	474, 630, 1317
SfuI	TTCGAA	1 cut(s)	1568
SinI	GGWCC	1 cut(s)	449
SmiI	ATTTAAAT	1 cut(s)	540
SmiMI	CAYNNNNRTG	1 cut(s)	1607
SmlI	CTYRAG	5 cut(s)	98, 279, 590, 832, 1070
SmoI	CTYRAG	5 cut(s)	98, 279, 590, 832, 1070
Sse9I	AATT	9 cut(s)	187, 600, 644, 795, 822, 969, 991, 1024, 1530
SseBI	AGGCCT	1 cut(s)	966
SstI	GAGCTC	1 cut(s)	259
StuI	AGGCCT	1 cut(s)	966
StyD4I	CCNGG	1 cut(s)	1545
StyI	CCWWGG	2 cut(s)	144, 997
SwaI	ATTTAAAT	1 cut(s)	540
TaaI	ACNGT	8 cut(s)	163, 676, 783, 856, 1093, 1151, 1574, 1609
TaiI	ACGT	3 cut(s)	728, 1431, 1658
TaqI	TCGA	2 cut(s)	513, 1568
TasI	AATT	9 cut(s)	187, 600, 644, 795, 822, 969, 991, 1024, 1530
TauI	GCSGC	3 cut(s)	684, 712, 770
TfiI	GAWTC	5 cut(s)	134, 341, 790, 950, 1157
Tru1I	TTAA	7 cut(s)	99, 539, 786, 957, 1035, 1334, 1644
Tru9I	TTAA	7 cut(s)	99, 539, 786, 957, 1035, 1334, 1644
TscAI	CASTG	6 cut(s)	160, 399, 691, 1051, 1108, 1518
TseFI	GTSAC	5 cut(s)	139, 577, 812, 914, 1451
TseI	GCWGC	5 cut(s)	167, 1250, 1541, 1550, 1553
Tsp45I	GTSAC	5 cut(s)	139, 577, 812, 914, 1451
TspDTI	ATGAA	6 cut(s)	300, 848, 962, 990, 1407, 1455
TspGWI	ACGGA	1 cut(s)	539
TspRI	CASTG	6 cut(s)	160, 399, 691, 1051, 1108, 1518
Vha464I	CTTAAG	1 cut(s)	98
VpaK11BI	GGWCC	1 cut(s)	449
XagI	CCTNNNNNAGG	1 cut(s)	456
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1289
XmiI	GTMKAC	2 cut(s)	14, 699
Zsp2I	ATGCAT	1 cut(s)	1346

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.