MD08G1095900.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011968
Physical Location & Seq
Forward (+)
8036698 .. 8037948
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1095900.v1.1.491

Sequence Viewer

Length: 1251 bp
ATGAAGCTCCTCTGCAACCCAATCCACACCTTCCCCTATAAACCCTTCCCTTCAATTTCCCTCCACTCATCCCGCTCCCCTTTCTTTCATTCAAAACGCGCGCTTCTACCCCAACCCATCATCACCACCGCCGCAACCTCAACCACCAGGAATTGCCGCCGCGGTGTCATCAAGTCATCCGCAAGCTCCGAAATTGACATGGTGCAAAACAAGCAGGGAATATACACGCCGAAGCAGAAGAAGGTTGTGATCTTGTGGGACCTGGACAACAAACCCCCACGCGGGCCGCCGTACCAGGCGGCCATGGCGCTGAAACAGCTCGCCCAGCGCTTCGGCGAGGTGGTCGACGCGTCGGCATACGCCAACCGCCACGCGTTTGTTCATTTGCCGCAGTGGGTCATCGAGGAGCGCCGCGAGCGGAAGCAACTGGACATTTTGGAGCGCAAGGGTGTGGTTACGCCGCCGGATCCGTACGTCTGCGGCGTCTGCGGGCGGAAGTGCAAGACGAATTTGGATTTGAAGAAGCATTTCAAGCAGCTGCACGAGAGGGAGAGGCAGAAGAAGCTGAATCGGATGGGGTCTTTGAAAGGGAAGAAACGCCAGCGTTTTAAGGAGAGGTTTGTTTCTGGGAATCACAAGTACAATGAGGCGGCGAGGTCTTTGATGAAGCCGAAGGTTGGGTACGGATTGGCGTCGGAGTTGCGGAGGGCGGGGGTTTTTGTGAAGACGGTGGAGGATAAGCCGCAGGCGGCGGATTGGGCGTTGAAGAGGCAAATGCAGCATTCTATGAGCCGAGGGATTGATTGGTTGTTCTTGGTTTCCGACGATTCGGACTTTTCGGATATGTTGAGGAAGGCTAGGGAGGCCAAGTTGGGGACTGTAGTGGTTGGGGACATGGATAGAGCTTTGGGAAGGCATGCTGATGTTTGGGTGCCCTGGATTGGAGTCGAAAATGGGGAGGTTTCGGAGAAAGATTTGGTTCCCAGAAGGAGGATAAGCGAGTTCTTTGATGGGGATGAGGAGGAAAATGGTGATGGGCTGTTTTCGGATTTTGGTTTTGATGGTGGGAGTGGATTAGACAATGTGGTTGATGAACTAGTGAGAAGTTCTCAGTCTGGTGGACTGAGGATTTCCGCCTTTTCGGAAGGGGAACTGGAAGGAGGAGACTGGGTGGAAGACGATTCGGATAGTGAGGAGTATCTGCTTGATAGTGAGAATGAGGATTCAGATGAGGAAGATGGGTTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NYN

Protein Analysis

417

Amino Acids

47.18

Weight (kDa)

7.67

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011968)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52010
fragaria_vesca FvH4_2g34000 FvH4_2g34000 FvH4_2g34000
malus_domestica MD08G1095900.v1.1 MD15G1078700.v1.1
prunus_persica Prupe.1G431900_v2.0.a1
pyrus_communis pycom08g07810 pycom15g07340
rosa_chinensis RchiOBHm_Chr6g0308381
rosa_laevigata RLG00000010646
rosa_multiflora Rmu_sc0023733.1_g000001
rosa_roxburghii Rroxscaffold_7G00160170
rosa_rugosa Rorug06G0366400
rosa_samantha Rh6AG475800 Rh6BG486100 Rh6CG491300 Rh6DG477600
rosa_wichuraiana Rw6G041470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 931
AccBSI CCGCTC 2 cut(s) 75, 418
AccI GTMKAC 1 cut(s) 345
AccII CGCG 7 cut(s) 99, 101, 162, 282, 350, 374, 414
AclWI GGATC 2 cut(s) 461, 474
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 1 cut(s) 508
AcyI GRCGYC 2 cut(s) 483, 692
AfaI GTAC 4 cut(s) 293, 473, 641, 683
AfeI AGCGCT 1 cut(s) 329
AfiI CCNNNNNNNGG 6 cut(s) 281, 282, 677, 873, 941, 990
AflIII ACRYGT 2 cut(s) 348, 372
AgsI TTSAA 6 cut(s) 54, 93, 520, 532, 586, 766
AhlI ACTAGT 1 cut(s) 1096
AjnI CCWGG 4 cut(s) 146, 261, 294, 935
AluBI AGCT 6 cut(s) 7, 186, 319, 538, 565, 905
AluI AGCT 6 cut(s) 7, 186, 319, 538, 565, 905
Alw26I GTCTC 1 cut(s) 1158
AlwI GGATC 2 cut(s) 461, 474
Aor51HI AGCGCT 1 cut(s) 329
AoxI GGCC 3 cut(s) 284, 300, 864
ApeKI GCWGC 3 cut(s) 535, 538, 778
ApoI RAATTY 1 cut(s) 508
Asp700I GAANNNNTTC 1 cut(s) 527
AspLEI GCGC 6 cut(s) 101, 103, 310, 330, 411, 444
AspS9I GGNCC 2 cut(s) 259, 284
AsuHPI GGTGA 2 cut(s) 115, 1043
AvaII GGWCC 1 cut(s) 259
BaeGI GKGCMC 1 cut(s) 936
BamHI GGATCC 1 cut(s) 466
BanI GGYRCC 1 cut(s) 931
BarI GAAGNNNNNNTAC 2 cut(s) 665, 697
BauI CACGAG 1 cut(s) 542
BbsI GAAGAC 2 cut(s) 731, 1182
BbvI GCAGC 3 cut(s) 525, 547, 790
BccI CCATC 6 cut(s) 125, 568, 1004, 1028, 1055, 1232
BceAI ACGGC 1 cut(s) 274
BciT130I CCWGG 4 cut(s) 148, 263, 296, 937
BcoDI GTCTC 1 cut(s) 1158
BcuI ACTAGT 1 cut(s) 1096
BfaI CTAG 2 cut(s) 858, 1097
BfmI CTRYAG 1 cut(s) 879
BfoI RGCGCY 3 cut(s) 311, 331, 412
Bme1390I CCNGG 4 cut(s) 148, 263, 296, 937
Bme18I GGWCC 1 cut(s) 259
BmgT120I GGNCC 2 cut(s) 259, 284
BmiI GGNNCC 4 cut(s) 260, 468, 933, 981
BmrFI CCNGG 4 cut(s) 148, 263, 296, 937
BmrI ACTGGG 1 cut(s) 1177
BmuI ACTGGG 1 cut(s) 1177
BpiI GAAGAC 2 cut(s) 731, 1182
BplI GAGNNNNNCTC 2 cut(s) 1093, 1125
BsaHI GRCGYC 2 cut(s) 483, 692
BsaJI CCNNGG 4 cut(s) 160, 303, 793, 935
BsaXI ACNNNNNCTCC 4 cut(s) 854, 884, 1155, 1185
Bsc4I CCNNNNNNNGG 6 cut(s) 281, 282, 677, 873, 941, 990
Bse1I ACTGG 3 cut(s) 432, 1158, 1172
BseBI CCWGG 4 cut(s) 148, 263, 296, 937
BseDI CCNNGG 4 cut(s) 160, 303, 793, 935
BseGI GGATG 4 cut(s) 68, 176, 579, 1021
BseLI CCNNNNNNNGG 6 cut(s) 281, 282, 677, 873, 941, 990
BseMII CTCAG 2 cut(s) 1115, 1124
BseNI ACTGG 3 cut(s) 432, 1158, 1172
BsePI GCGCGC 1 cut(s) 99
BseRI GAGGAG 4 cut(s) 419, 1034, 1176, 1208
BseSI GKGCMC 1 cut(s) 936
BseXI GCAGC 3 cut(s) 525, 547, 790
BseYI CCCAGC 1 cut(s) 324
BsgI GTGCAG 1 cut(s) 524
Bsh1236I CGCG 7 cut(s) 99, 101, 162, 282, 350, 374, 414
BshFI GGCC 3 cut(s) 286, 302, 866
BshNI GGYRCC 1 cut(s) 931
BsiSI CCGG 1 cut(s) 464
BsiWI CGTACG 1 cut(s) 471
BslFI GGGAC 3 cut(s) 272, 889, 905
BslI CCNNNNNNNGG 6 cut(s) 281, 282, 677, 873, 941, 990
BsmAI GTCTC 1 cut(s) 1158
BsmFI GGGAC 3 cut(s) 272, 889, 905
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 3 cut(s) 286, 302, 866
Bsp1286I GDGCHC 1 cut(s) 936
Bsp143I GATC 2 cut(s) 249, 466
Bsp19I CCATGG 1 cut(s) 303
BspANI GGCC 3 cut(s) 286, 302, 866
BspCNI CTCAG 2 cut(s) 1116, 1123
BspFNI CGCG 7 cut(s) 99, 101, 162, 282, 350, 374, 414
BspLI GGNNCC 4 cut(s) 260, 468, 933, 981
BspPI GGATC 2 cut(s) 461, 474
BspT107I GGYRCC 1 cut(s) 931
BsrBI CCGCTC 2 cut(s) 75, 418
BsrI ACTGG 3 cut(s) 432, 1158, 1172
BssECI CCNNGG 4 cut(s) 160, 303, 793, 935
BssHII GCGCGC 1 cut(s) 99
BssMI GATC 2 cut(s) 249, 466
BssNI GRCGYC 2 cut(s) 483, 692
BssSI CACGAG 1 cut(s) 542
BssT1I CCWWGG 1 cut(s) 303
Bst2BI CACGAG 1 cut(s) 542
Bst2UI CCWGG 4 cut(s) 148, 263, 296, 937
Bst4CI ACNGT 2 cut(s) 730, 880
Bst6I CTCTTC 1 cut(s) 761
BstACI GRCGYC 2 cut(s) 483, 692
BstC8I GCNNGC 9 cut(s) 101, 184, 284, 321, 416, 491, 602, 747, 918
BstDEI CTNAG 2 cut(s) 1110, 1124
BstDSI CCRYGG 2 cut(s) 160, 303
BstF5I GGATG 4 cut(s) 68, 176, 579, 1021
BstFNI CGCG 7 cut(s) 99, 101, 162, 282, 350, 374, 414
BstH2I RGCGCY 3 cut(s) 311, 331, 412
BstHHI GCGC 6 cut(s) 101, 103, 310, 330, 411, 444
BstKTI GATC 2 cut(s) 252, 469
BstMAI GTCTC 1 cut(s) 1158
BstMBI GATC 2 cut(s) 249, 466
BstNI CCWGG 4 cut(s) 148, 263, 296, 937
BstNSI RCATGY 1 cut(s) 920
BstSCI CCNGG 4 cut(s) 146, 261, 294, 935
BstSFI CTRYAG 1 cut(s) 879
BstSLI GKGCMC 1 cut(s) 936
BstUI CGCG 7 cut(s) 99, 101, 162, 282, 350, 374, 414
BstV1I GCAGC 3 cut(s) 525, 547, 790
BstV2I GAAGAC 2 cut(s) 731, 1182
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BsuRI GGCC 3 cut(s) 286, 302, 866
BtgI CCRYGG 2 cut(s) 160, 303
BtsCI GGATG 4 cut(s) 68, 176, 579, 1021
BtsI GCAGTG 1 cut(s) 398
BtsIMutI CAGTG 1 cut(s) 398
Cac8I GCNNGC 9 cut(s) 101, 184, 284, 321, 416, 491, 602, 747, 918
CfoI GCGC 6 cut(s) 101, 103, 310, 330, 411, 444
Cfr13I GGNCC 2 cut(s) 259, 284
Cfr42I CCGCGG 1 cut(s) 163
CseI GACGC 4 cut(s) 339, 356, 472, 681
Csp6I GTAC 4 cut(s) 292, 472, 640, 682
CviAII CATG 4 cut(s) 199, 304, 895, 917
CviQI GTAC 4 cut(s) 292, 472, 640, 682
DdeI CTNAG 2 cut(s) 1110, 1124
DpnI GATC 2 cut(s) 251, 468
DpnII GATC 2 cut(s) 249, 466
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 1 cut(s) 761
EarI CTCTTC 1 cut(s) 761
EciI GGCGGA 3 cut(s) 508, 767, 1123
Eco130I CCWWGG 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 259
Eco47III AGCGCT 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 259
EcoRII CCWGG 4 cut(s) 146, 261, 294, 935
EcoT14I CCWWGG 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 4 cut(s) 202, 307, 898, 920
FaiI YATR 9 cut(s) 39, 200, 223, 305, 358, 788, 845, 896, 918
FaqI GGGAC 3 cut(s) 272, 889, 905
FatI CATG 4 cut(s) 198, 303, 894, 916
FauI CCCGC 4 cut(s) 80, 275, 482, 703
FblI GTMKAC 1 cut(s) 345
FokI GGATG 4 cut(s) 55, 163, 586, 1028
FspBI CTAG 2 cut(s) 858, 1097
GlaI GCGC 6 cut(s) 100, 102, 309, 329, 410, 443
GsaI CCCAGC 1 cut(s) 328
HaeII RGCGCY 3 cut(s) 311, 331, 412
HaeIII GGCC 3 cut(s) 286, 302, 866
HapII CCGG 1 cut(s) 464
HgaI GACGC 4 cut(s) 339, 356, 472, 681
HhaI GCGC 6 cut(s) 101, 103, 310, 330, 411, 444
Hin1I GRCGYC 2 cut(s) 483, 692
Hin1II CATG 4 cut(s) 202, 307, 898, 920
Hin6I GCGC 6 cut(s) 99, 101, 308, 328, 409, 442
HinP1I GCGC 6 cut(s) 99, 101, 308, 328, 409, 442
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 6 cut(s) 568, 631, 827, 945, 1181, 1223
HpaII CCGG 1 cut(s) 464
HphI GGTGA 2 cut(s) 115, 1043
Hpy166II GTNNAC 2 cut(s) 346, 1121
Hpy8I GTNNAC 2 cut(s) 346, 1121
Hpy99I CGWCG 4 cut(s) 350, 355, 697, 827
HpyCH4III ACNGT 2 cut(s) 730, 880
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 5 cut(s) 15, 205, 501, 541, 778
HpyF3I CTNAG 2 cut(s) 1110, 1124
HpySE526I ACGT 1 cut(s) 474
Hsp92I GRCGYC 2 cut(s) 483, 692
Hsp92II CATG 4 cut(s) 202, 307, 898, 920
HspAI GCGC 6 cut(s) 99, 101, 308, 328, 409, 442
KspI CCGCGG 1 cut(s) 163
Kzo9I GATC 2 cut(s) 249, 466
LmnI GCTCC 5 cut(s) 12, 80, 191, 406, 439
Lsp1109I GCAGC 3 cut(s) 525, 547, 790
MaeI CTAG 2 cut(s) 858, 1097
MaeII ACGT 1 cut(s) 474
MaeIII GTNAC 1 cut(s) 454
MalI GATC 2 cut(s) 251, 468
MbiI CCGCTC 2 cut(s) 75, 418
MboI GATC 2 cut(s) 249, 466
MboII GAAGA 8 cut(s) 250, 532, 571, 604, 736, 778, 1187, 1247
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 1 cut(s) 936
MluCI AATT 4 cut(s) 54, 151, 192, 508
MluI ACGCGT 2 cut(s) 348, 372
MlyI GAGTC 1 cut(s) 954
MmeI TCCRAC 2 cut(s) 675, 846
MroXI GAANNNNTTC 1 cut(s) 527
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 1 cut(s) 921
MspA1I CMGCKG 2 cut(s) 162, 538
MspI CCGG 1 cut(s) 464
MspR9I CCNGG 4 cut(s) 148, 263, 296, 937
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 4 cut(s) 148, 263, 296, 937
MvnI CGCG 7 cut(s) 99, 101, 162, 282, 350, 374, 414
NcoI CCATGG 1 cut(s) 303
NdeII GATC 2 cut(s) 249, 466
NlaIII CATG 4 cut(s) 202, 307, 898, 920
NlaIV GGNNCC 4 cut(s) 260, 468, 933, 981
NmeAIII GCCGAG 1 cut(s) 818
NspI RCATGY 1 cut(s) 920
PaeI GCATGC 1 cut(s) 920
PauI GCGCGC 1 cut(s) 99
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PctI GAATGC 1 cut(s) 781
PdmI GAANNNNTTC 1 cut(s) 527
PfeI GAWTC 5 cut(s) 568, 631, 827, 1181, 1223
Pfl23II CGTACG 1 cut(s) 471
PleI GAGTC 1 cut(s) 953
PpsI GAGTC 1 cut(s) 953
PpuMI RGGWCCY 1 cut(s) 259
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 4 cut(s) 146, 261, 294, 935
PspFI CCCAGC 1 cut(s) 324
PspGI CCWGG 4 cut(s) 146, 261, 294, 935
PspLI CGTACG 1 cut(s) 471
PspN4I GGNNCC 4 cut(s) 260, 468, 933, 981
PspPI GGNCC 2 cut(s) 259, 284
PspPPI RGGWCCY 1 cut(s) 259
PsuI RGATCY 1 cut(s) 466
PteI GCGCGC 1 cut(s) 99
PvuII CAGCTG 1 cut(s) 538
RsaI GTAC 4 cut(s) 293, 473, 641, 683
RsaNI GTAC 4 cut(s) 292, 472, 640, 682
RseI CAYNNNNRTG 1 cut(s) 921
SacII CCGCGG 1 cut(s) 163
SalI GTCGAC 1 cut(s) 344
SaqAI TTAA 1 cut(s) 609
Sau3AI GATC 2 cut(s) 249, 466
Sau96I GGNCC 2 cut(s) 259, 284
SchI GAGTC 1 cut(s) 954
ScrFI CCNGG 4 cut(s) 148, 263, 296, 937
SduI GDGCHC 1 cut(s) 936
SfcI CTRYAG 1 cut(s) 879
Sfr303I CCGCGG 1 cut(s) 163
SgrBI CCGCGG 1 cut(s) 163
SinI GGWCC 1 cut(s) 259
SmiMI CAYNNNNRTG 1 cut(s) 921
SpeI ACTAGT 1 cut(s) 1096
SphI GCATGC 1 cut(s) 920
Sse9I AATT 4 cut(s) 54, 151, 192, 508
SspMI CTAG 2 cut(s) 858, 1097
StyD4I CCNGG 4 cut(s) 146, 261, 294, 935
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 2 cut(s) 730, 880
TaiI ACGT 1 cut(s) 477
TaqI TCGA 3 cut(s) 345, 402, 948
TasI AATT 4 cut(s) 54, 151, 192, 508
TatI WGTACW 1 cut(s) 639
TfiI GAWTC 5 cut(s) 568, 631, 827, 1181, 1223
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 1 cut(s) 398
TseI GCWGC 3 cut(s) 535, 538, 778
TspDTI ATGAA 5 cut(s) 17, 77, 371, 680, 1107
TspGWI ACGGA 2 cut(s) 459, 699
TspRI CASTG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 259
XapI RAATTY 1 cut(s) 508
XceI RCATGY 1 cut(s) 920
XmiI GTMKAC 1 cut(s) 345
XmnI GAANNNNTTC 1 cut(s) 527
XspI CTAG 2 cut(s) 858, 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.