pycom08g07810

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011968
Physical Location & Seq
Forward (+)
6273186 .. 6274436
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g07810.1

Sequence Viewer

Length: 1251 bp
ATGAAGCTCCTCTGCAACCCAATCCACACCTTCCCCTATAAACCCTTCCTTCCAATTTCTCTCCACTCCTCCCGCTCCCCTTTCTTTCATTCAAAACGCGCGCTTCTATCCCAACCCATCATCACCACCGCCGCAACATCAACCACCAGGAATTGCCGCCGCGTTGTCATCAAGTCATCCGCAAGCTCCGAAATTGACATGGTGCAAAACAAGCAGGGAATATACACGCCGAAGCAGAAGAAAGTTGTGATCTTGTGGGACCTGGACAACAAACCCCCTCGCGGACCGCCGTACCAGGCGGCCATGGCGCTGAAACAGCTCGCCCAGCGCTTCGGCGAGGTGGTCGACGCGTCGGCATACGCCAACCGCCACGCGTTTGTTCATTTGCCGCAGTGGGTCATCGAGGAGCGCCGCGACCGGAAGCAACTGGACATTTTGGAGCGCAAGGGTGTGGTTTCGCCGCCGGATCCGTACGTCTGCGGCGTCTGCGGGCGGAAGTGCAAGACGAATTTGGATTTAAAGAAACATTTCAAGCAGCTGCACGAGAGGGAGAGGCAGAAGAAGCTGAATCGGATGGGGTCTTTGAAAGGGAAGAAACGCCAGCGTTTTAAGGAGAGGTTTGTTTCTGGGAATCACAAGTACAATGAGGCGGCGAGGTCTTTGATCAAGCCGAAGGTTGGGTACGGATTGGCGTCGGAGTTGAGGAGGGCGGGGGTTTTTGTGAAGACGGTGGAGGATAAGCCGCAGGCGGCGGATTGGGCGTTGAAGAGGCAAATGCAGCATTCTATGAGCCGAGGGATTGATTGGCTGTTCTTGGTTTCGGACGATTCGGACTTTTCGAATATGTTGAGGAAGGCTAGGGAGGCCAAGTTGGGGACTGTGGTGGTTGGGGATTTGGATAGAGCTTTGGGAAGGCATGCTGATGTTTGGGTGCCGTGGGTTGGTGTCGAAAATGGGGAGGTTTCGGAGAAAGATTTGGTACCGAGAAGGAGGATAAGTGAGTTCTTTGATGGGGATGAGGAGGAAAATAGTGATGGGCTGTTTTCGGATTTCGGTTTTGATGGTGGGAGTGGATTAGACAATGCGGTTGATGAACTAGTGAGAAGTTCTCAGTCTGGTGGACTGAGGATTTCCGCCTTTTCGGAAGGGGAACTGGAAGGAGGAGACTGGGTGGAAGACGATTCGGATAGTGAGGAGTATCTGCTTGATAGTGAGAATGAGGATTCAGATGAGGAAGATGGGTTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NYN

Protein Analysis

417

Amino Acids

47.16

Weight (kDa)

8.14

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011968)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52010
fragaria_vesca FvH4_2g34000 FvH4_2g34000 FvH4_2g34000
malus_domestica MD08G1095900.v1.1 MD15G1078700.v1.1
prunus_persica Prupe.1G431900_v2.0.a1
pyrus_communis pycom08g07810 pycom15g07340
rosa_chinensis RchiOBHm_Chr6g0308381
rosa_laevigata RLG00000010646
rosa_multiflora Rmu_sc0023733.1_g000001
rosa_roxburghii Rroxscaffold_7G00160170
rosa_rugosa Rorug06G0366400
rosa_samantha Rh6AG475800 Rh6BG486100 Rh6CG491300 Rh6DG477600
rosa_wichuraiana Rw6G041470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 979
AccB1I GGYRCC 2 cut(s) 931, 979
AccBSI CCGCTC 1 cut(s) 75
AccI GTMKAC 1 cut(s) 345
AccII CGCG 7 cut(s) 99, 101, 162, 282, 350, 374, 414
AclWI GGATC 2 cut(s) 461, 474
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 1 cut(s) 508
AcyI GRCGYC 2 cut(s) 483, 692
AfaI GTAC 5 cut(s) 293, 473, 641, 683, 981
AfeI AGCGCT 1 cut(s) 329
AfiI CCNNNNNNNGG 4 cut(s) 281, 677, 873, 941
AflIII ACRYGT 2 cut(s) 348, 372
AgsI TTSAA 4 cut(s) 93, 532, 586, 766
AhlI ACTAGT 1 cut(s) 1096
AjnI CCWGG 3 cut(s) 146, 261, 294
AluBI AGCT 6 cut(s) 7, 186, 319, 538, 565, 905
AluI AGCT 6 cut(s) 7, 186, 319, 538, 565, 905
Alw26I GTCTC 1 cut(s) 1158
AlwI GGATC 2 cut(s) 461, 474
Aor51HI AGCGCT 1 cut(s) 329
AoxI GGCC 2 cut(s) 300, 864
ApeKI GCWGC 3 cut(s) 535, 538, 778
ApoI RAATTY 1 cut(s) 508
Asp700I GAANNNNTTC 1 cut(s) 527
Asp718I GGTACC 1 cut(s) 979
AspLEI GCGC 6 cut(s) 101, 103, 310, 330, 411, 444
AspS9I GGNCC 2 cut(s) 259, 284
AsuHPI GGTGA 1 cut(s) 115
AsuII TTCGAA 1 cut(s) 839
AvaII GGWCC 2 cut(s) 259, 284
BaeI ACNNNNGTAYC 2 cut(s) 275, 308
BamHI GGATCC 1 cut(s) 466
BanI GGYRCC 2 cut(s) 931, 979
BarI GAAGNNNNNNTAC 2 cut(s) 665, 697
BauI CACGAG 1 cut(s) 542
BbsI GAAGAC 2 cut(s) 731, 1182
BbvI GCAGC 3 cut(s) 525, 547, 790
BccI CCATC 6 cut(s) 125, 568, 1004, 1028, 1055, 1232
BceAI ACGGC 2 cut(s) 274, 919
BciT130I CCWGG 3 cut(s) 148, 263, 296
BclI TGATCA 1 cut(s) 663
BcoDI GTCTC 1 cut(s) 1158
BcuI ACTAGT 1 cut(s) 1096
BfaI CTAG 2 cut(s) 858, 1097
BfoI RGCGCY 3 cut(s) 311, 331, 412
Bme1390I CCNGG 3 cut(s) 148, 263, 296
Bme18I GGWCC 2 cut(s) 259, 284
BmgT120I GGNCC 2 cut(s) 259, 284
BmiI GGNNCC 4 cut(s) 260, 468, 933, 981
BmrFI CCNGG 3 cut(s) 148, 263, 296
BmrI ACTGGG 1 cut(s) 1177
BmuI ACTGGG 1 cut(s) 1177
BpiI GAAGAC 2 cut(s) 731, 1182
BplI GAGNNNNNCTC 2 cut(s) 1093, 1125
Bpu14I TTCGAA 1 cut(s) 839
BsaHI GRCGYC 2 cut(s) 483, 692
BsaJI CCNNGG 3 cut(s) 303, 793, 935
BsaWI WCCGGW 1 cut(s) 417
BsaXI ACNNNNNCTCC 6 cut(s) 854, 884, 982, 1012, 1155, 1185
Bsc4I CCNNNNNNNGG 4 cut(s) 281, 677, 873, 941
Bse1I ACTGG 3 cut(s) 432, 1158, 1172
BseBI CCWGG 3 cut(s) 148, 263, 296
BseDI CCNNGG 3 cut(s) 303, 793, 935
BseGI GGATG 3 cut(s) 176, 579, 1021
BseLI CCNNNNNNNGG 4 cut(s) 281, 677, 873, 941
BseMII CTCAG 2 cut(s) 1115, 1124
BseNI ACTGG 3 cut(s) 432, 1158, 1172
BsePI GCGCGC 1 cut(s) 99
BseRI GAGGAG 6 cut(s) 58, 419, 718, 1034, 1176, 1208
BseXI GCAGC 3 cut(s) 525, 547, 790
BseYI CCCAGC 1 cut(s) 324
BsgI GTGCAG 1 cut(s) 524
Bsh1236I CGCG 7 cut(s) 99, 101, 162, 282, 350, 374, 414
Bsh1285I CGRYCG 1 cut(s) 418
BshFI GGCC 2 cut(s) 302, 866
BshNI GGYRCC 2 cut(s) 931, 979
BsiEI CGRYCG 1 cut(s) 418
BsiSI CCGG 2 cut(s) 418, 464
BsiWI CGTACG 1 cut(s) 471
BslFI GGGAC 2 cut(s) 272, 889
BslI CCNNNNNNNGG 4 cut(s) 281, 677, 873, 941
BsmAI GTCTC 1 cut(s) 1158
BsmFI GGGAC 2 cut(s) 272, 889
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 2 cut(s) 302, 866
Bsp119I TTCGAA 1 cut(s) 839
Bsp143I GATC 3 cut(s) 249, 466, 663
Bsp19I CCATGG 1 cut(s) 303
BspANI GGCC 2 cut(s) 302, 866
BspCNI CTCAG 2 cut(s) 1116, 1123
BspFNI CGCG 7 cut(s) 99, 101, 162, 282, 350, 374, 414
BspLI GGNNCC 4 cut(s) 260, 468, 933, 981
BspPI GGATC 2 cut(s) 461, 474
BspT104I TTCGAA 1 cut(s) 839
BspT107I GGYRCC 2 cut(s) 931, 979
BsrBI CCGCTC 1 cut(s) 75
BsrI ACTGG 3 cut(s) 432, 1158, 1172
BssECI CCNNGG 3 cut(s) 303, 793, 935
BssHII GCGCGC 1 cut(s) 99
BssMI GATC 3 cut(s) 249, 466, 663
BssNI GRCGYC 2 cut(s) 483, 692
BssSI CACGAG 1 cut(s) 542
BssT1I CCWWGG 1 cut(s) 303
Bst2BI CACGAG 1 cut(s) 542
Bst2UI CCWGG 3 cut(s) 148, 263, 296
Bst4CI ACNGT 2 cut(s) 730, 880
Bst6I CTCTTC 1 cut(s) 761
BstACI GRCGYC 2 cut(s) 483, 692
BstBI TTCGAA 1 cut(s) 839
BstC8I GCNNGC 7 cut(s) 101, 184, 321, 491, 602, 747, 918
BstDEI CTNAG 2 cut(s) 1110, 1124
BstDSI CCRYGG 2 cut(s) 303, 935
BstF5I GGATG 3 cut(s) 176, 579, 1021
BstFNI CGCG 7 cut(s) 99, 101, 162, 282, 350, 374, 414
BstH2I RGCGCY 3 cut(s) 311, 331, 412
BstHHI GCGC 6 cut(s) 101, 103, 310, 330, 411, 444
BstKTI GATC 3 cut(s) 252, 469, 666
BstMAI GTCTC 1 cut(s) 1158
BstMBI GATC 3 cut(s) 249, 466, 663
BstMCI CGRYCG 1 cut(s) 418
BstMWI GCNNNNNNNGC 9 cut(s) 211, 305, 316, 325, 486, 562, 758, 778, 863
BstNI CCWGG 3 cut(s) 148, 263, 296
BstNSI RCATGY 1 cut(s) 920
BstSCI CCNGG 3 cut(s) 146, 261, 294
BstUI CGCG 7 cut(s) 99, 101, 162, 282, 350, 374, 414
BstV1I GCAGC 3 cut(s) 525, 547, 790
BstV2I GAAGAC 2 cut(s) 731, 1182
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BsuRI GGCC 2 cut(s) 302, 866
BtgI CCRYGG 2 cut(s) 303, 935
BtsCI GGATG 3 cut(s) 176, 579, 1021
BtsI GCAGTG 1 cut(s) 398
BtsIMutI CAGTG 1 cut(s) 398
Cac8I GCNNGC 7 cut(s) 101, 184, 321, 491, 602, 747, 918
CfoI GCGC 6 cut(s) 101, 103, 310, 330, 411, 444
Cfr13I GGNCC 2 cut(s) 259, 284
CpoI CGGWCCG 1 cut(s) 284
CseI GACGC 4 cut(s) 339, 356, 472, 681
Csp6I GTAC 5 cut(s) 292, 472, 640, 682, 980
CspI CGGWCCG 1 cut(s) 284
CviAII CATG 3 cut(s) 199, 304, 917
CviQI GTAC 5 cut(s) 292, 472, 640, 682, 980
DdeI CTNAG 2 cut(s) 1110, 1124
DpnI GATC 3 cut(s) 251, 468, 665
DpnII GATC 3 cut(s) 249, 466, 663
DraI TTTAAA 1 cut(s) 519
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 1 cut(s) 761
EarI CTCTTC 1 cut(s) 761
EciI GGCGGA 3 cut(s) 508, 767, 1123
Eco130I CCWWGG 1 cut(s) 303
Eco47I GGWCC 2 cut(s) 259, 284
Eco47III AGCGCT 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 259
EcoRII CCWGG 3 cut(s) 146, 261, 294
EcoT14I CCWWGG 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 3 cut(s) 202, 307, 920
FaiI YATR 8 cut(s) 39, 200, 223, 305, 358, 788, 845, 918
FaqI GGGAC 2 cut(s) 272, 889
FatI CATG 3 cut(s) 198, 303, 916
FauI CCCGC 3 cut(s) 80, 482, 703
FbaI TGATCA 1 cut(s) 663
FblI GTMKAC 1 cut(s) 345
FokI GGATG 3 cut(s) 163, 586, 1028
FspBI CTAG 2 cut(s) 858, 1097
GlaI GCGC 6 cut(s) 100, 102, 309, 329, 410, 443
GsaI CCCAGC 1 cut(s) 328
HaeII RGCGCY 3 cut(s) 311, 331, 412
HaeIII GGCC 2 cut(s) 302, 866
HapII CCGG 2 cut(s) 418, 464
HgaI GACGC 4 cut(s) 339, 356, 472, 681
HhaI GCGC 6 cut(s) 101, 103, 310, 330, 411, 444
Hin1I GRCGYC 2 cut(s) 483, 692
Hin1II CATG 3 cut(s) 202, 307, 920
Hin6I GCGC 6 cut(s) 99, 101, 308, 328, 409, 442
HinP1I GCGC 6 cut(s) 99, 101, 308, 328, 409, 442
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 5 cut(s) 568, 631, 827, 1181, 1223
HpaII CCGG 2 cut(s) 418, 464
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 2 cut(s) 346, 1121
Hpy8I GTNNAC 2 cut(s) 346, 1121
Hpy99I CGWCG 3 cut(s) 350, 355, 697
HpyAV CCTTC 9 cut(s) 40, 55, 59, 667, 847, 906, 981, 1139, 1151
HpyCH4III ACNGT 2 cut(s) 730, 880
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 5 cut(s) 15, 205, 501, 541, 778
HpyF10VI GCNNNNNNNGC 9 cut(s) 211, 305, 316, 325, 486, 562, 758, 778, 863
HpyF3I CTNAG 2 cut(s) 1110, 1124
HpySE526I ACGT 1 cut(s) 474
Hsp92I GRCGYC 2 cut(s) 483, 692
Hsp92II CATG 3 cut(s) 202, 307, 920
HspAI GCGC 6 cut(s) 99, 101, 308, 328, 409, 442
KpnI GGTACC 1 cut(s) 983
Ksp22I TGATCA 1 cut(s) 663
Kzo9I GATC 3 cut(s) 249, 466, 663
LmnI GCTCC 5 cut(s) 12, 80, 191, 406, 439
Lsp1109I GCAGC 3 cut(s) 525, 547, 790
MaeI CTAG 2 cut(s) 858, 1097
MaeII ACGT 1 cut(s) 474
MalI GATC 3 cut(s) 251, 468, 665
MbiI CCGCTC 1 cut(s) 75
MboI GATC 3 cut(s) 249, 466, 663
MboII GAAGA 7 cut(s) 250, 571, 604, 736, 778, 1187, 1247
MflI RGATCY 1 cut(s) 466
MluCI AATT 4 cut(s) 54, 151, 192, 508
MluI ACGCGT 2 cut(s) 348, 372
MmeI TCCRAC 1 cut(s) 675
MroXI GAANNNNTTC 1 cut(s) 527
MseI TTAA 2 cut(s) 518, 609
MslI CAYNNNNRTG 1 cut(s) 921
MspA1I CMGCKG 1 cut(s) 538
MspI CCGG 2 cut(s) 418, 464
MspR9I CCNGG 3 cut(s) 148, 263, 296
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 3 cut(s) 148, 263, 296
MvnI CGCG 7 cut(s) 99, 101, 162, 282, 350, 374, 414
MwoI GCNNNNNNNGC 9 cut(s) 211, 305, 316, 325, 486, 562, 758, 778, 863
NcoI CCATGG 1 cut(s) 303
NdeII GATC 3 cut(s) 249, 466, 663
NlaIII CATG 3 cut(s) 202, 307, 920
NlaIV GGNNCC 4 cut(s) 260, 468, 933, 981
NmeAIII GCCGAG 1 cut(s) 818
NspI RCATGY 1 cut(s) 920
NspV TTCGAA 1 cut(s) 839
PaeI GCATGC 1 cut(s) 920
PauI GCGCGC 1 cut(s) 99
PcsI WCGNNNNNNNCGW 2 cut(s) 480, 836
PctI GAATGC 1 cut(s) 781
PdmI GAANNNNTTC 1 cut(s) 527
PfeI GAWTC 5 cut(s) 568, 631, 827, 1181, 1223
Pfl23II CGTACG 1 cut(s) 471
PpuMI RGGWCCY 1 cut(s) 259
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 3 cut(s) 146, 261, 294
PspFI CCCAGC 1 cut(s) 324
PspGI CCWGG 3 cut(s) 146, 261, 294
PspLI CGTACG 1 cut(s) 471
PspN4I GGNNCC 4 cut(s) 260, 468, 933, 981
PspPI GGNCC 2 cut(s) 259, 284
PspPPI RGGWCCY 1 cut(s) 259
PsuI RGATCY 1 cut(s) 466
PteI GCGCGC 1 cut(s) 99
PvuII CAGCTG 1 cut(s) 538
RsaI GTAC 5 cut(s) 293, 473, 641, 683, 981
RsaNI GTAC 5 cut(s) 292, 472, 640, 682, 980
RseI CAYNNNNRTG 1 cut(s) 921
Rsr2I CGGWCCG 1 cut(s) 284
RsrII CGGWCCG 1 cut(s) 284
SalI GTCGAC 1 cut(s) 344
SaqAI TTAA 2 cut(s) 518, 609
Sau3AI GATC 3 cut(s) 249, 466, 663
Sau96I GGNCC 2 cut(s) 259, 284
ScrFI CCNGG 3 cut(s) 148, 263, 296
SfuI TTCGAA 1 cut(s) 839
SinI GGWCC 2 cut(s) 259, 284
SmiMI CAYNNNNRTG 1 cut(s) 921
SpeI ACTAGT 1 cut(s) 1096
SphI GCATGC 1 cut(s) 920
Sse9I AATT 4 cut(s) 54, 151, 192, 508
SspMI CTAG 2 cut(s) 858, 1097
StyD4I CCNGG 3 cut(s) 146, 261, 294
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 2 cut(s) 730, 880
TaiI ACGT 1 cut(s) 477
TaqI TCGA 4 cut(s) 345, 402, 839, 948
TasI AATT 4 cut(s) 54, 151, 192, 508
TatI WGTACW 1 cut(s) 639
TfiI GAWTC 5 cut(s) 568, 631, 827, 1181, 1223
Tru1I TTAA 2 cut(s) 518, 609
Tru9I TTAA 2 cut(s) 518, 609
TscAI CASTG 1 cut(s) 398
TseI GCWGC 3 cut(s) 535, 538, 778
TspDTI ATGAA 4 cut(s) 17, 77, 371, 1107
TspGWI ACGGA 2 cut(s) 459, 699
TspRI CASTG 1 cut(s) 398
VpaK11BI GGWCC 2 cut(s) 259, 284
XapI RAATTY 1 cut(s) 508
XceI RCATGY 1 cut(s) 920
XmiI GTMKAC 1 cut(s) 345
XmnI GAANNNNTTC 1 cut(s) 527
XspI CTAG 2 cut(s) 858, 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.