Rh6DG477600

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0011968
Physical Location & Seq
Reverse (-)
64290707 .. 64294869
4163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG477600.1

Sequence Viewer

Length: 1236 bp
ATGGTGAACCACATCATCTGCAACCCAATACACACCACCCTCCCTTATAACAAACCCCTTCCTCTCCTTTTCCTCAATTCCTCCCAATCCCCATTCTTCAATTCCCAAATCAACCCAAAACACTCGTCCTCAATTTCCTTTAACAAACGAAGATGGAGGAGGAGCTATACAGCTGCCGCGAACTCCGAAATCGACATGGTGAGAAACAAGCAAGGCATATACACGCCGAAACAGAAGAAAGTGGTGATTTTATGGGACCTGGACAACAAACCCCCTCGCGGGCCGCCGTACCAGGCGGCCATGGCGCTGAAACAGGTGGCCCAGCACTTCGGCGAGGTAGTTGACGTGTCGGCCTACGCCAACCGCCACGCCTTCATCCACTTGCCCAACTGGGTAGTGGAGCAGCGCCGGGAACGGAAGCAATTAGACATTCTGGAGCGCAAGGGGATAGTCACGCCTCCCGAGCCTTACATTTGCGGAGTCTGCGGCCGGAAGTGTAAGTCGAATTTGGATTTGACGAAGCACTTCAAGCAGCTACACGAGCGGGAGCGGCAGAAGAAGCTGAACCGAATGCGGTCTCTGAAAGGGAAGAAACGGCAGCGTTTCAAGGAGAGGTTTATTTCGGGGAATCATAAGTACAATGTGGCTGCGAGTACTTTGATCAAGCCCAAGGTTGGGTATGGACTGGCGGCTGAGCTGAGAAGAGCTGGGGTTTTTGTGAAGACGGTGGAGGATAAGCCGCAGGCGGCGGATTGGGCATTGAAGAGGCAAATGCAGCATTCTATGAGCCGTGGGATTGACTGGTTGTTCTTGGTTTCGGACGATTCGGATTTTTCGGACATGTTGAGGAAGGCTAGAGAGTCCACTTTGGGGACTGTGGTGGTGGGGGATTGGGATAGAGCTTTGGGGAGGCATGCGGATGTGTGGGTGCCGTGGACTGAGGTCGAAAAGGGGGAGATTTCCGAGAAGGATTTGGTGCCGAAAAGGAGGAGAAGTGAGTTCTCTGATGAGGATTCCGTGTTTTCGGATTTGGGATTTGGTGGTGGGAGTGAATTGGACAATGTGGTTGATGATCTTGTTGGACTAAGGTCTCAGGGGTCTGGTGGAATGAGGATTTCCGCATTTTCAGAAGGGGAAGTGGAAGAAGGAGATTGGGAGGAAGACTATGAGGATGGGGACTATTTGTTGGAGGACAGCGAGGATGAGGATTCAGAAGATGAAGATGGGTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NYN

Protein Analysis

411

Amino Acids

47.1

Weight (kDa)

7.18

Isoelectric Point (pI)

54.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011968)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52010
fragaria_vesca FvH4_2g34000 FvH4_2g34000 FvH4_2g34000
malus_domestica MD08G1095900.v1.1 MD15G1078700.v1.1
prunus_persica Prupe.1G431900_v2.0.a1
pyrus_communis pycom08g07810 pycom15g07340
rosa_chinensis RchiOBHm_Chr6g0308381
rosa_laevigata RLG00000010646
rosa_multiflora Rmu_sc0023733.1_g000001
rosa_roxburghii Rroxscaffold_7G00160170
rosa_rugosa Rorug06G0366400
rosa_samantha Rh6AG475800 Rh6BG486100 Rh6CG491300 Rh6DG477600
rosa_wichuraiana Rw6G041470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 48
AccB1I GGYRCC 2 cut(s) 928, 976
AccBSI CCGCTC 2 cut(s) 544, 550
AccII CGCG 2 cut(s) 179, 279
AcoI YGGCCR 2 cut(s) 297, 487
AcsI RAATTY 1 cut(s) 505
AfaI GTAC 3 cut(s) 290, 638, 655
AfiI CCNNNNNNNGG 5 cut(s) 278, 279, 674, 675, 870
AflIII ACRYGT 2 cut(s) 345, 840
AgsI TTSAA 4 cut(s) 100, 529, 607, 763
AjiI CACGTC 1 cut(s) 346
AjnI CCWGG 2 cut(s) 258, 291
AluBI AGCT 7 cut(s) 165, 173, 535, 562, 697, 707, 902
AluI AGCT 7 cut(s) 165, 173, 535, 562, 697, 707, 902
Alw26I GTCTC 2 cut(s) 582, 1095
Ama87I CYCGRG 1 cut(s) 461
AoxI GGCC 5 cut(s) 281, 297, 318, 351, 487
ApeKI GCWGC 6 cut(s) 173, 403, 532, 598, 647, 775
ApoI RAATTY 1 cut(s) 505
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
Asp700I GAANNNNTTC 1 cut(s) 524
AspLEI GCGC 3 cut(s) 307, 408, 441
AspS9I GGNCC 3 cut(s) 256, 281, 319
AsuC2I CCSGG 1 cut(s) 410
AsuHPI GGTGA 3 cut(s) 16, 211, 256
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 1 cut(s) 256
BanI GGYRCC 2 cut(s) 928, 976
BauI CACGAG 1 cut(s) 539
BbsI GAAGAC 2 cut(s) 728, 1167
BbvI GCAGC 6 cut(s) 160, 415, 544, 610, 634, 787
BccI CCATC 3 cut(s) 147, 1166, 1217
BceAI ACGGC 4 cut(s) 271, 611, 774, 916
BciT130I CCWGG 2 cut(s) 260, 293
BclI TGATCA 1 cut(s) 660
BcnI CCSGG 1 cut(s) 410
BcoDI GTCTC 2 cut(s) 582, 1095
BfaI CTAG 1 cut(s) 855
BfoI RGCGCY 2 cut(s) 308, 409
BlpI GCTNAGC 1 cut(s) 693
BmcAI AGTACT 1 cut(s) 655
Bme1390I CCNGG 3 cut(s) 260, 293, 410
Bme18I GGWCC 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 461
BmgBI CACGTC 1 cut(s) 346
BmgT120I GGNCC 3 cut(s) 256, 281, 319
BmiI GGNNCC 3 cut(s) 257, 930, 978
BmrFI CCNGG 3 cut(s) 260, 293, 410
BmrI ACTGGG 1 cut(s) 400
BmuI ACTGGG 1 cut(s) 400
BoxI GACNNNNGTC 2 cut(s) 941, 1087
BpiI GAAGAC 2 cut(s) 728, 1167
BpmI CTGGAG 1 cut(s) 455
Bpu1102I GCTNAGC 1 cut(s) 693
BpuMI CCSGG 1 cut(s) 410
BsaI GGTCTC 2 cut(s) 582, 1095
BsaJI CCNNGG 4 cut(s) 300, 669, 790, 932
BsaXI ACNNNNNCTCC 2 cut(s) 979, 1009
Bsc4I CCNNNNNNNGG 5 cut(s) 278, 279, 674, 675, 870
Bse1I ACTGG 3 cut(s) 395, 690, 806
BseBI CCWGG 2 cut(s) 260, 293
BseDI CCNNGG 4 cut(s) 300, 669, 790, 932
BseGI GGATG 4 cut(s) 375, 925, 1177, 1207
BseLI CCNNNNNNNGG 5 cut(s) 278, 279, 674, 675, 870
BseMII CTCAG 4 cut(s) 684, 689, 930, 1106
BseNI ACTGG 3 cut(s) 395, 690, 806
BseRI GAGGAG 3 cut(s) 172, 175, 1003
BseX3I CGGCCG 1 cut(s) 487
BseXI GCAGC 6 cut(s) 160, 415, 544, 610, 634, 787
BseYI CCCAGC 2 cut(s) 321, 707
Bsh1236I CGCG 2 cut(s) 179, 279
Bsh1285I CGRYCG 1 cut(s) 490
BshFI GGCC 5 cut(s) 283, 299, 320, 353, 489
BshNI GGYRCC 2 cut(s) 928, 976
BsiEI CGRYCG 1 cut(s) 490
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 2 cut(s) 409, 490
BslFI GGGAC 3 cut(s) 269, 886, 1190
BslI CCNNNNNNNGG 5 cut(s) 278, 279, 674, 675, 870
BsmAI GTCTC 2 cut(s) 582, 1095
BsmFI GGGAC 3 cut(s) 269, 886, 1190
BsmI GAATGC 2 cut(s) 576, 778
BsnI GGCC 5 cut(s) 283, 299, 320, 353, 489
Bso31I GGTCTC 2 cut(s) 582, 1095
BsoBI CYCGRG 1 cut(s) 461
Bsp143I GATC 2 cut(s) 660, 1072
Bsp1720I GCTNAGC 1 cut(s) 693
Bsp19I CCATGG 1 cut(s) 300
BspANI GGCC 5 cut(s) 283, 299, 320, 353, 489
BspCNI CTCAG 4 cut(s) 685, 690, 931, 1105
BspFNI CGCG 2 cut(s) 179, 279
BspLI GGNNCC 3 cut(s) 257, 930, 978
BspQI GCTCTTC 1 cut(s) 697
BspT107I GGYRCC 2 cut(s) 928, 976
BspTNI GGTCTC 2 cut(s) 582, 1095
BsrBI CCGCTC 2 cut(s) 544, 550
BsrI ACTGG 3 cut(s) 395, 690, 806
BssECI CCNNGG 4 cut(s) 300, 669, 790, 932
BssMI GATC 2 cut(s) 660, 1072
BssSI CACGAG 1 cut(s) 539
BssT1I CCWWGG 2 cut(s) 300, 669
Bst2BI CACGAG 1 cut(s) 539
Bst2UI CCWGG 2 cut(s) 260, 293
Bst4CI ACNGT 2 cut(s) 727, 877
Bst6I CTCTTC 2 cut(s) 697, 758
BstC8I GCNNGC 3 cut(s) 281, 744, 915
BstDEI CTNAG 5 cut(s) 693, 698, 939, 1085, 1092
BstDSI CCRYGG 3 cut(s) 300, 790, 932
BstF5I GGATG 4 cut(s) 375, 925, 1177, 1207
BstFNI CGCG 2 cut(s) 179, 279
BstH2I RGCGCY 2 cut(s) 308, 409
BstHHI GCGC 3 cut(s) 307, 408, 441
BstKTI GATC 2 cut(s) 663, 1075
BstMAI GTCTC 2 cut(s) 582, 1095
BstMBI GATC 2 cut(s) 660, 1072
BstMCI CGRYCG 1 cut(s) 490
BstMWI GCNNNNNNNGC 9 cut(s) 302, 463, 483, 529, 541, 550, 559, 755, 775
BstNI CCWGG 2 cut(s) 260, 293
BstNSI RCATGY 2 cut(s) 844, 917
BstPAI GACNNNNGTC 2 cut(s) 941, 1087
BstSCI CCNGG 3 cut(s) 258, 291, 408
BstUI CGCG 2 cut(s) 179, 279
BstV1I GCAGC 6 cut(s) 160, 415, 544, 610, 634, 787
BstV2I GAAGAC 2 cut(s) 728, 1167
BstZI CGGCCG 1 cut(s) 487
BsuRI GGCC 5 cut(s) 283, 299, 320, 353, 489
BtgI CCRYGG 3 cut(s) 300, 790, 932
BtrI CACGTC 1 cut(s) 346
BtsCI GGATG 4 cut(s) 375, 925, 1177, 1207
Cac8I GCNNGC 3 cut(s) 281, 744, 915
CfoI GCGC 3 cut(s) 307, 408, 441
Cfr13I GGNCC 3 cut(s) 256, 281, 319
Csp6I GTAC 3 cut(s) 289, 637, 654
CviAII CATG 4 cut(s) 196, 301, 841, 914
CviQI GTAC 3 cut(s) 289, 637, 654
DdeI CTNAG 5 cut(s) 693, 698, 939, 1085, 1092
DpnI GATC 2 cut(s) 662, 1074
DpnII GATC 2 cut(s) 660, 1072
EaeI YGGCCR 2 cut(s) 297, 487
EagI CGGCCG 1 cut(s) 487
Eam1104I CTCTTC 2 cut(s) 697, 758
EarI CTCTTC 2 cut(s) 697, 758
EciI GGCGGA 1 cut(s) 764
EclXI CGGCCG 1 cut(s) 487
Eco130I CCWWGG 2 cut(s) 300, 669
Eco31I GGTCTC 2 cut(s) 582, 1095
Eco47I GGWCC 1 cut(s) 256
Eco52I CGGCCG 1 cut(s) 487
Eco88I CYCGRG 1 cut(s) 461
EcoO109I RGGNCCY 1 cut(s) 256
EcoRII CCWGG 2 cut(s) 258, 291
EcoT14I CCWWGG 2 cut(s) 300, 669
ErhI CCWWGG 2 cut(s) 300, 669
FaeI CATG 4 cut(s) 199, 304, 844, 917
FaqI GGGAC 3 cut(s) 269, 886, 1190
FatI CATG 4 cut(s) 195, 300, 840, 913
FauI CCCGC 2 cut(s) 272, 537
FbaI TGATCA 1 cut(s) 660
FokI GGATG 4 cut(s) 362, 932, 1184, 1214
FspBI CTAG 1 cut(s) 855
GlaI GCGC 3 cut(s) 306, 407, 440
GsaI CCCAGC 2 cut(s) 325, 711
GsuI CTGGAG 1 cut(s) 455
HaeII RGCGCY 2 cut(s) 308, 409
HaeIII GGCC 5 cut(s) 283, 299, 320, 353, 489
HapII CCGG 2 cut(s) 409, 490
HhaI GCGC 3 cut(s) 307, 408, 441
Hin1II CATG 4 cut(s) 199, 304, 844, 917
Hin6I GCGC 3 cut(s) 305, 406, 439
HinP1I GCGC 3 cut(s) 305, 406, 439
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 6 cut(s) 480, 628, 824, 860, 1013, 1208
HpaII CCGG 2 cut(s) 409, 490
HphI GGTGA 3 cut(s) 16, 211, 256
Hpy166II GTNNAC 4 cut(s) 7, 343, 864, 936
Hpy188III TCNNGA 2 cut(s) 434, 461
Hpy8I GTNNAC 4 cut(s) 7, 343, 864, 936
HpyAV CCTTC 6 cut(s) 68, 382, 844, 961, 1124, 1139
HpyCH4III ACNGT 2 cut(s) 727, 877
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 2 cut(s) 21, 775
HpyF10VI GCNNNNNNNGC 9 cut(s) 302, 463, 483, 529, 541, 550, 559, 755, 775
HpyF3I CTNAG 5 cut(s) 693, 698, 939, 1085, 1092
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 4 cut(s) 199, 304, 844, 917
HspAI GCGC 3 cut(s) 305, 406, 439
Ksp22I TGATCA 1 cut(s) 660
Kzo9I GATC 2 cut(s) 660, 1072
LguI GCTCTTC 1 cut(s) 697
LmnI GCTCC 4 cut(s) 162, 400, 436, 547
Lsp1109I GCAGC 6 cut(s) 160, 415, 544, 610, 634, 787
MaeI CTAG 1 cut(s) 855
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 1 cut(s) 451
MalI GATC 2 cut(s) 662, 1074
MbiI CCGCTC 2 cut(s) 544, 550
MboI GATC 2 cut(s) 660, 1072
MluCI AATT 6 cut(s) 76, 100, 132, 422, 505, 1052
MlyI GAGTC 2 cut(s) 489, 869
MmeI TCCRAC 2 cut(s) 1060, 1167
MroXI GAANNNNTTC 1 cut(s) 524
MseI TTAA 1 cut(s) 141
MslI CAYNNNNRTG 1 cut(s) 918
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 2 cut(s) 409, 490
MspR9I CCNGG 3 cut(s) 260, 293, 410
Mva1269I GAATGC 2 cut(s) 576, 778
MvaI CCWGG 2 cut(s) 260, 293
MvnI CGCG 2 cut(s) 179, 279
MwoI GCNNNNNNNGC 9 cut(s) 302, 463, 483, 529, 541, 550, 559, 755, 775
NciI CCSGG 1 cut(s) 410
NcoI CCATGG 1 cut(s) 300
NdeII GATC 2 cut(s) 660, 1072
NlaIII CATG 4 cut(s) 199, 304, 844, 917
NlaIV GGNNCC 3 cut(s) 257, 930, 978
NmuCI GTSAC 1 cut(s) 451
NspI RCATGY 2 cut(s) 844, 917
PaeI GCATGC 1 cut(s) 917
PciI ACATGT 1 cut(s) 840
PciSI GCTCTTC 1 cut(s) 697
PctI GAATGC 2 cut(s) 576, 778
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 4 cut(s) 628, 824, 1013, 1208
PleI GAGTC 2 cut(s) 488, 868
PpsI GAGTC 2 cut(s) 488, 868
PpuMI RGGWCCY 1 cut(s) 256
PscI ACATGT 1 cut(s) 840
PshAI GACNNNNGTC 2 cut(s) 941, 1087
PsiI TTATAA 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 256
Psp6I CCWGG 2 cut(s) 258, 291
PspFI CCCAGC 2 cut(s) 321, 707
PspGI CCWGG 2 cut(s) 258, 291
PspN4I GGNNCC 3 cut(s) 257, 930, 978
PspPI GGNCC 3 cut(s) 256, 281, 319
PspPPI RGGWCCY 1 cut(s) 256
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 3 cut(s) 290, 638, 655
RsaNI GTAC 3 cut(s) 289, 637, 654
RseI CAYNNNNRTG 1 cut(s) 918
SapI GCTCTTC 1 cut(s) 697
SaqAI TTAA 1 cut(s) 141
Sau3AI GATC 2 cut(s) 660, 1072
Sau96I GGNCC 3 cut(s) 256, 281, 319
ScaI AGTACT 1 cut(s) 655
SchI GAGTC 2 cut(s) 489, 869
ScrFI CCNGG 3 cut(s) 260, 293, 410
SinI GGWCC 1 cut(s) 256
SmiMI CAYNNNNRTG 1 cut(s) 918
SphI GCATGC 1 cut(s) 917
Sse9I AATT 6 cut(s) 76, 100, 132, 422, 505, 1052
SspMI CTAG 1 cut(s) 855
StyD4I CCNGG 3 cut(s) 258, 291, 408
StyI CCWWGG 2 cut(s) 300, 669
TaaI ACNGT 2 cut(s) 727, 877
TaiI ACGT 1 cut(s) 348
TaqI TCGA 3 cut(s) 192, 503, 945
TasI AATT 6 cut(s) 76, 100, 132, 422, 505, 1052
TatI WGTACW 2 cut(s) 636, 653
TauI GCSGC 8 cut(s) 179, 286, 299, 489, 553, 692, 742, 749
TfiI GAWTC 4 cut(s) 628, 824, 1013, 1208
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 6 cut(s) 173, 403, 532, 598, 647, 775
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 2 cut(s) 364, 1233
TspGWI ACGGA 2 cut(s) 430, 1006
VpaK11BI GGWCC 1 cut(s) 256
XapI RAATTY 1 cut(s) 505
XceI RCATGY 2 cut(s) 844, 917
XcmI CCANNNNNNNNNTGG 1 cut(s) 394
XmnI GAANNNNTTC 1 cut(s) 524
XspI CTAG 1 cut(s) 855
ZrmI AGTACT 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.