MD08G1099700.v1.1

Protein DCL, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010127
Physical Location & Seq
Reverse (-)
8381656 .. 8383620
1965 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1099700.v1.1.491

Sequence Viewer

Length: 645 bp
ATGGCATCTCTCTCCAGATCACCGCCGCTCCTCCTCCGCCACATAAACTCCCTCACCCTCCACCCCTGCCCCCTAACTCTATCCTTCCCTTTCCTCAATACGACGTTGCCTCACCCCCGCCCCTGCGCCCTGAAGACGGGACCCGACGGGGGCAGCAGAATCGGGAGGCCCGGGACGCAGGGGCCCGACCCGGGACTGCTCCGAAAGCCGGTGGTGTCGTCCGGGAAGGACATGGACGGGATTTCGGAGGAAGATGGGGGAGAAGATGGGAAGTGGGTCGATTGGGAGGATAAGATTTTGGAGGATACAGTTCCGCTGGTAGGGTTTGTGAGGATGATTCTTCATTCGGGGAAGTACGAGAGCGGAGATAGATTGAGTCCTGAGCATCAGAAGACTGTTCTCGAAAGGTTGCTTCCGTTTCATCCTGAAGCTAAGAAGAAGATCGGTTCTGGAATCGATTATATCACGGTTGGTTATCACCCTGATTTTGAAAGCTCAAGATGTTTGTTCATTGTTCAAAAGGACGGAACACTAGTCGACTTTTCATATTGGAAATGCATAAAGGGGCTGATAAGGAAGAACTATCCTTTATACGCAGACAGCTTCATTCTCAGACATTTCCGAAAGCGCAGACGCGGTTTATGA

Protein Analysis

215

Amino Acids

24.06

Weight (kDa)

9.16

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 111 - 187 3.1e-30 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 28, 363
AccI GTMKAC 1 cut(s) 537
AccII CGCG 1 cut(s) 636
AciI CCGC 7 cut(s) 23, 26, 37, 118, 314, 363, 636
AcuI CTGAAG 2 cut(s) 152, 447
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 5 cut(s) 136, 149, 191, 208, 320
AgsI TTSAA 2 cut(s) 491, 518
AhlI ACTAGT 1 cut(s) 532
AluBI AGCT 3 cut(s) 431, 495, 603
AluI AGCT 3 cut(s) 431, 495, 603
Ama87I CYCGRG 2 cut(s) 170, 190
AoxI GGCC 2 cut(s) 167, 182
ApaI GGGCCC 1 cut(s) 186
ApeKI GCWGC 1 cut(s) 153
AspLEI GCGC 2 cut(s) 128, 630
AspS9I GGNCC 4 cut(s) 140, 168, 182, 183
AsuC2I CCSGG 5 cut(s) 171, 172, 191, 192, 223
AsuHPI GGTGA 4 cut(s) 12, 46, 104, 470
AvaI CYCGRG 2 cut(s) 170, 190
AvaII GGWCC 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 186
BanII GRGCYC 1 cut(s) 186
BbsI GAAGAC 2 cut(s) 140, 398
BbvI GCAGC 1 cut(s) 165
BccI CCATC 2 cut(s) 248, 260
BcgI CGANNNNNNTGC 2 cut(s) 142, 176
BciVI GTATCC 1 cut(s) 298
BcnI CCSGG 5 cut(s) 171, 172, 191, 192, 223
BcuI ACTAGT 1 cut(s) 532
BfaI CTAG 1 cut(s) 533
BfuI GTATCC 1 cut(s) 298
BisI GCNGC 2 cut(s) 26, 154
BlsI GCNGC 2 cut(s) 27, 155
Bme1390I CCNGG 5 cut(s) 171, 172, 191, 192, 223
Bme18I GGWCC 1 cut(s) 140
BmeT110I CYCGRG 2 cut(s) 170, 190
BmgT120I GGNCC 4 cut(s) 140, 168, 182, 183
BmiI GGNNCC 4 cut(s) 141, 142, 183, 184
BmrFI CCNGG 5 cut(s) 171, 172, 191, 192, 223
BmsI GCATC 2 cut(s) 14, 394
BpiI GAAGAC 2 cut(s) 140, 398
Bpu10I CCTNAGC 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 481
BpuMI CCSGG 5 cut(s) 171, 172, 191, 192, 223
Bsa29I ATCGAT 1 cut(s) 456
BsaJI CCNNGG 2 cut(s) 170, 190
BsaXI ACNNNNNCTCC 4 cut(s) 12, 32, 42, 62
Bsc4I CCNNNNNNNGG 5 cut(s) 136, 149, 191, 208, 320
Bse118I RCCGGY 1 cut(s) 208
BseCI ATCGAT 1 cut(s) 456
BseDI CCNNGG 2 cut(s) 170, 190
BseGI GGATG 2 cut(s) 339, 421
BseLI CCNNNNNNNGG 5 cut(s) 136, 149, 191, 208, 320
BseMII CTCAG 2 cut(s) 372, 625
BseRI GAGGAG 2 cut(s) 20, 23
BseSI GKGCMC 1 cut(s) 186
BseXI GCAGC 1 cut(s) 165
Bsh1236I CGCG 1 cut(s) 636
BshFI GGCC 2 cut(s) 169, 184
BshVI ATCGAT 1 cut(s) 456
BsiHKCI CYCGRG 2 cut(s) 170, 190
BsiSI CCGG 4 cut(s) 171, 191, 209, 222
BslFI GGGAC 3 cut(s) 153, 187, 207
BslI CCNNNNNNNGG 5 cut(s) 136, 149, 191, 208, 320
BsmFI GGGAC 3 cut(s) 153, 187, 207
BsnI GGCC 2 cut(s) 169, 184
BsoBI CYCGRG 2 cut(s) 170, 190
Bsp120I GGGCCC 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 2 cut(s) 17, 441
BspACI CCGC 7 cut(s) 23, 26, 37, 118, 314, 363, 636
BspANI GGCC 2 cut(s) 169, 184
BspCNI CTCAG 2 cut(s) 373, 624
BspDI ATCGAT 1 cut(s) 456
BspFNI CGCG 1 cut(s) 636
BspLI GGNNCC 4 cut(s) 141, 142, 183, 184
BsrBI CCGCTC 2 cut(s) 28, 363
BsrFI RCCGGY 1 cut(s) 208
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 2 cut(s) 170, 190
BssMI GATC 2 cut(s) 17, 441
Bst4CI ACNGT 3 cut(s) 310, 397, 469
BstDEI CTNAG 3 cut(s) 381, 432, 611
BstF5I GGATG 2 cut(s) 339, 421
BstFNI CGCG 1 cut(s) 636
BstHHI GCGC 2 cut(s) 128, 630
BstKTI GATC 2 cut(s) 20, 444
BstMBI GATC 2 cut(s) 17, 441
BstMWI GCNNNNNNNGC 2 cut(s) 175, 205
BstSCI CCNGG 5 cut(s) 169, 170, 189, 190, 221
BstSLI GKGCMC 1 cut(s) 186
BstUI CGCG 1 cut(s) 636
BstV1I GCAGC 1 cut(s) 165
BstV2I GAAGAC 2 cut(s) 140, 398
Bsu15I ATCGAT 1 cut(s) 456
BsuI GTATCC 1 cut(s) 298
BsuRI GGCC 2 cut(s) 169, 184
BsuTUI ATCGAT 1 cut(s) 456
BtsCI GGATG 2 cut(s) 339, 421
CfoI GCGC 2 cut(s) 128, 630
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 4 cut(s) 140, 168, 182, 183
Cfr9I CCCGGG 2 cut(s) 170, 190
ClaI ATCGAT 1 cut(s) 456
CseI GACGC 1 cut(s) 184
Csp6I GTAC 1 cut(s) 355
CviAII CATG 1 cut(s) 232
CviJI RGCY 7 cut(s) 169, 184, 208, 431, 495, 568, 603
CviKI_1 RGCY 7 cut(s) 169, 184, 208, 431, 495, 568, 603
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 3 cut(s) 381, 432, 611
DpnI GATC 2 cut(s) 19, 443
DpnII GATC 2 cut(s) 17, 441
EciI GGCGGA 1 cut(s) 26
Eco24I GRGCYC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 140
Eco57I CTGAAG 2 cut(s) 152, 447
Eco88I CYCGRG 2 cut(s) 170, 190
EcoO109I RGGNCCY 2 cut(s) 140, 182
EcoT22I ATGCAT 1 cut(s) 560
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 1 cut(s) 235
FaiI YATR 7 cut(s) 44, 233, 462, 547, 560, 592, 643
FaqI GGGAC 3 cut(s) 153, 187, 207
FatI CATG 1 cut(s) 231
FauI CCCGC 1 cut(s) 125
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 2 cut(s) 26, 154
FokI GGATG 2 cut(s) 346, 408
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 2 cut(s) 26, 154
FspBI CTAG 1 cut(s) 533
GlaI GCGC 2 cut(s) 127, 629
GluI GCNGC 2 cut(s) 26, 154
HaeIII GGCC 2 cut(s) 169, 184
HapII CCGG 4 cut(s) 171, 191, 209, 222
HgaI GACGC 1 cut(s) 184
HhaI GCGC 2 cut(s) 128, 630
Hin1II CATG 1 cut(s) 235
Hin6I GCGC 2 cut(s) 126, 628
HinP1I GCGC 2 cut(s) 126, 628
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HinfI GANTC 4 cut(s) 159, 337, 376, 453
HpaII CCGG 4 cut(s) 171, 191, 209, 222
HphI GGTGA 4 cut(s) 12, 46, 104, 470
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 5 cut(s) 203, 247, 390, 614, 623
Hpy188III TCNNGA 7 cut(s) 15, 163, 380, 401, 425, 450, 498
Hpy8I GTNNAC 1 cut(s) 538
Hpy99I CGWCG 2 cut(s) 106, 149
HpyAV CCTTC 2 cut(s) 94, 220
HpyCH4III ACNGT 3 cut(s) 310, 397, 469
HpyCH4IV ACGT 1 cut(s) 104
HpyCH4V TGCA 1 cut(s) 558
HpyF10VI GCNNNNNNNGC 2 cut(s) 175, 205
HpyF3I CTNAG 3 cut(s) 381, 432, 611
HpySE526I ACGT 1 cut(s) 104
Hsp92II CATG 1 cut(s) 235
HspAI GCGC 2 cut(s) 126, 628
KflI GGGWCCC 1 cut(s) 140
Kzo9I GATC 2 cut(s) 17, 441
LmnI GCTCC 2 cut(s) 33, 204
Lsp1109I GCAGC 1 cut(s) 165
LweI GCATC 2 cut(s) 14, 394
MaeI CTAG 1 cut(s) 533
MaeII ACGT 1 cut(s) 104
MalI GATC 2 cut(s) 19, 443
MbiI CCGCTC 2 cut(s) 28, 363
MboI GATC 2 cut(s) 17, 441
MboII GAAGA 8 cut(s) 145, 263, 275, 332, 403, 448, 451, 589
MhlI GDGCHC 1 cut(s) 186
MlyI GAGTC 1 cut(s) 385
Mph1103I ATGCAT 1 cut(s) 560
MspA1I CMGCKG 1 cut(s) 316
MspI CCGG 4 cut(s) 171, 191, 209, 222
MspR9I CCNGG 5 cut(s) 171, 172, 191, 192, 223
MvnI CGCG 1 cut(s) 636
MwoI GCNNNNNNNGC 2 cut(s) 175, 205
NciI CCSGG 5 cut(s) 171, 172, 191, 192, 223
NdeII GATC 2 cut(s) 17, 441
NlaIII CATG 1 cut(s) 235
NlaIV GGNNCC 4 cut(s) 141, 142, 183, 184
NsiI ATGCAT 1 cut(s) 560
PfeI GAWTC 3 cut(s) 159, 337, 453
PfoI TCCNGGA 1 cut(s) 221
PkrI GCNGC 2 cut(s) 27, 155
PleI GAGTC 1 cut(s) 384
PpsI GAGTC 1 cut(s) 384
PpuMI RGGWCCY 1 cut(s) 140
Psp5II RGGWCCY 1 cut(s) 140
PspN4I GGNNCC 4 cut(s) 141, 142, 183, 184
PspOMI GGGCCC 1 cut(s) 182
PspPI GGNCC 4 cut(s) 140, 168, 182, 183
PspPPI RGGWCCY 1 cut(s) 140
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
SalI GTCGAC 1 cut(s) 536
SatI GCNGC 2 cut(s) 26, 154
Sau3AI GATC 2 cut(s) 17, 441
Sau96I GGNCC 4 cut(s) 140, 168, 182, 183
SchI GAGTC 1 cut(s) 385
ScrFI CCNGG 5 cut(s) 171, 172, 191, 192, 223
SduI GDGCHC 1 cut(s) 186
SetI ASST 5 cut(s) 107, 410, 433, 497, 605
SfaNI GCATC 2 cut(s) 14, 394
SinI GGWCC 1 cut(s) 140
SmaI CCCGGG 2 cut(s) 172, 192
SmlI CTYRAG 1 cut(s) 496
SmoI CTYRAG 1 cut(s) 496
SpeI ACTAGT 1 cut(s) 532
SsiI CCGC 7 cut(s) 23, 26, 37, 118, 314, 363, 636
SspMI CTAG 1 cut(s) 533
StyD4I CCNGG 5 cut(s) 169, 170, 189, 190, 221
TaaI ACNGT 3 cut(s) 310, 397, 469
TaiI ACGT 1 cut(s) 107
TaqI TCGA 4 cut(s) 279, 402, 456, 537
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 3 cut(s) 159, 337, 453
TseI GCWGC 1 cut(s) 153
TspDTI ATGAA 5 cut(s) 332, 410, 499, 534, 595
TspGWI ACGGA 2 cut(s) 405, 540
TspMI CCCGGG 2 cut(s) 170, 190
VpaK11BI GGWCC 1 cut(s) 140
XmaI CCCGGG 2 cut(s) 170, 190
XmiI GTMKAC 1 cut(s) 537
XspI CTAG 1 cut(s) 533
Zsp2I ATGCAT 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.