pycom08g08100

Protein DCL, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010127
Physical Location & Seq
Reverse (-)
6542137 .. 6543777
1641 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g08100.1

Sequence Viewer

Length: 633 bp
ATGGCATCTCTCTCCAGATCACCGCCGCTCCTCCACCGCCACTTAAACTCCCTCACCCTCCACCCCTGCCCCCTAACTCTATCCTTCCCTTTCCTCAAAACGACGTTACCTTACCCCCGCCCCTGCGCCCTGAAGACGGGACCCAACGGGGGTAGCAGAATCGGGAGGCCCGAGACGCAGGGGCCCGACCCGGGACTGCTCCGAAAGCCGGTGGTGTCGTCCGGGAAGGACTTGGACGGGATTTCGGAGGAAGATGGAAAGTGGGTCGATTGGGAGGATAAGATTTTGGAGGACACAGTTCCGCTGGTAGGGTTTGTGAGGATGATTCTTCATTCGGGGAAGTACGAGAGCGGAGATAGATTGAGTCCTGAGCATCAGAAGACTGTTCTCGAAAGGTTGCTTCCGTTTCATCCTGAAGCTAAGAAGAAGATCGGTTCTGGAATCGATTATATCACGGTTGGTTATCACCCTGATTTTGAAAGCTCAAGATGTTTGTTCATTGTTCAAAAGGACGGAACACTAGTCGACTTTTCATATTGGAAATGCATAAAGGGGCTGATAAGGAAGAACTATCCTTTATACGCAGACAGCTTCATTCTCAGACATTTCCGAAAGCGCAGACGTGGTTTATGA

Protein Analysis

211

Amino Acids

23.82

Weight (kDa)

9.45

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 107 - 183 3e-30 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 28, 351
AccI GTMKAC 1 cut(s) 525
AciI CCGC 6 cut(s) 23, 26, 37, 118, 302, 351
AcuI CTGAAG 2 cut(s) 152, 435
AfaI GTAC 1 cut(s) 344
AfiI CCNNNNNNNGG 5 cut(s) 136, 149, 191, 208, 308
AgsI TTSAA 2 cut(s) 479, 506
AhlI ACTAGT 1 cut(s) 520
AjiI CACGTC 1 cut(s) 623
AluBI AGCT 3 cut(s) 419, 483, 591
AluI AGCT 3 cut(s) 419, 483, 591
Alw26I GTCTC 1 cut(s) 167
Ama87I CYCGRG 2 cut(s) 170, 190
AoxI GGCC 2 cut(s) 167, 182
ApaI GGGCCC 1 cut(s) 186
AspLEI GCGC 2 cut(s) 128, 618
AspS9I GGNCC 4 cut(s) 140, 168, 182, 183
AsuC2I CCSGG 3 cut(s) 191, 192, 223
AsuHPI GGTGA 3 cut(s) 12, 46, 458
AvaI CYCGRG 2 cut(s) 170, 190
AvaII GGWCC 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 186
BanII GRGCYC 1 cut(s) 186
BbsI GAAGAC 2 cut(s) 140, 386
BccI CCATC 1 cut(s) 248
BcnI CCSGG 3 cut(s) 191, 192, 223
BcoDI GTCTC 1 cut(s) 167
BcuI ACTAGT 1 cut(s) 520
BfaI CTAG 1 cut(s) 521
BisI GCNGC 1 cut(s) 26
BlsI GCNGC 1 cut(s) 27
Bme1390I CCNGG 3 cut(s) 191, 192, 223
Bme18I GGWCC 1 cut(s) 140
BmeT110I CYCGRG 2 cut(s) 170, 190
BmgBI CACGTC 1 cut(s) 623
BmgT120I GGNCC 4 cut(s) 140, 168, 182, 183
BmiI GGNNCC 4 cut(s) 141, 142, 183, 184
BmrFI CCNGG 3 cut(s) 191, 192, 223
BmsI GCATC 2 cut(s) 14, 382
BpiI GAAGAC 2 cut(s) 140, 386
Bpu10I CCTNAGC 1 cut(s) 369
BpuEI CTTGAG 1 cut(s) 469
BpuMI CCSGG 3 cut(s) 191, 192, 223
Bsa29I ATCGAT 1 cut(s) 444
BsaJI CCNNGG 1 cut(s) 190
BsaXI ACNNNNNCTCC 4 cut(s) 12, 32, 42, 62
Bsc4I CCNNNNNNNGG 5 cut(s) 136, 149, 191, 208, 308
Bse118I RCCGGY 1 cut(s) 208
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 1 cut(s) 190
BseGI GGATG 2 cut(s) 327, 409
BseLI CCNNNNNNNGG 5 cut(s) 136, 149, 191, 208, 308
BseMII CTCAG 2 cut(s) 360, 613
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 186
BshFI GGCC 2 cut(s) 169, 184
BshVI ATCGAT 1 cut(s) 444
BsiHKCI CYCGRG 2 cut(s) 170, 190
BsiSI CCGG 3 cut(s) 191, 209, 222
BslFI GGGAC 2 cut(s) 153, 207
BslI CCNNNNNNNGG 5 cut(s) 136, 149, 191, 208, 308
BsmAI GTCTC 1 cut(s) 167
BsmBI CGTCTC 1 cut(s) 167
BsmFI GGGAC 2 cut(s) 153, 207
BsnI GGCC 2 cut(s) 169, 184
BsoBI CYCGRG 2 cut(s) 170, 190
Bsp120I GGGCCC 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 2 cut(s) 17, 429
BspACI CCGC 6 cut(s) 23, 26, 37, 118, 302, 351
BspANI GGCC 2 cut(s) 169, 184
BspCNI CTCAG 2 cut(s) 361, 612
BspDI ATCGAT 1 cut(s) 444
BspLI GGNNCC 4 cut(s) 141, 142, 183, 184
BsrBI CCGCTC 2 cut(s) 28, 351
BsrFI RCCGGY 1 cut(s) 208
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 1 cut(s) 190
BssMI GATC 2 cut(s) 17, 429
Bst4CI ACNGT 3 cut(s) 298, 385, 457
BstDEI CTNAG 3 cut(s) 369, 420, 599
BstF5I GGATG 2 cut(s) 327, 409
BstHHI GCGC 2 cut(s) 128, 618
BstKTI GATC 2 cut(s) 20, 432
BstMAI GTCTC 1 cut(s) 167
BstMBI GATC 2 cut(s) 17, 429
BstMWI GCNNNNNNNGC 2 cut(s) 175, 205
BstSCI CCNGG 3 cut(s) 189, 190, 221
BstSLI GKGCMC 1 cut(s) 186
BstV2I GAAGAC 2 cut(s) 140, 386
Bsu15I ATCGAT 1 cut(s) 444
BsuRI GGCC 2 cut(s) 169, 184
BsuTUI ATCGAT 1 cut(s) 444
BtrI CACGTC 1 cut(s) 623
BtsCI GGATG 2 cut(s) 327, 409
CfoI GCGC 2 cut(s) 128, 618
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 4 cut(s) 140, 168, 182, 183
Cfr9I CCCGGG 1 cut(s) 190
ClaI ATCGAT 1 cut(s) 444
CseI GACGC 1 cut(s) 184
Csp6I GTAC 1 cut(s) 343
CviJI RGCY 7 cut(s) 169, 184, 208, 419, 483, 556, 591
CviKI_1 RGCY 7 cut(s) 169, 184, 208, 419, 483, 556, 591
CviQI GTAC 1 cut(s) 343
DdeI CTNAG 3 cut(s) 369, 420, 599
DpnI GATC 2 cut(s) 19, 431
DpnII GATC 2 cut(s) 17, 429
Eco24I GRGCYC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 140
Eco57I CTGAAG 2 cut(s) 152, 435
Eco88I CYCGRG 2 cut(s) 170, 190
EcoO109I RGGNCCY 2 cut(s) 140, 182
EcoT22I ATGCAT 1 cut(s) 548
EcoT38I GRGCYC 1 cut(s) 186
Esp3I CGTCTC 1 cut(s) 167
FaiI YATR 5 cut(s) 450, 535, 548, 580, 631
FaqI GGGAC 2 cut(s) 153, 207
FauI CCCGC 1 cut(s) 125
FblI GTMKAC 1 cut(s) 525
Fnu4HI GCNGC 1 cut(s) 26
FokI GGATG 2 cut(s) 334, 396
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 1 cut(s) 26
FspBI CTAG 1 cut(s) 521
GlaI GCGC 2 cut(s) 127, 617
GluI GCNGC 1 cut(s) 26
HaeIII GGCC 2 cut(s) 169, 184
HapII CCGG 3 cut(s) 191, 209, 222
HgaI GACGC 1 cut(s) 184
HhaI GCGC 2 cut(s) 128, 618
Hin6I GCGC 2 cut(s) 126, 616
HinP1I GCGC 2 cut(s) 126, 616
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 4 cut(s) 159, 325, 364, 441
HpaII CCGG 3 cut(s) 191, 209, 222
HphI GGTGA 3 cut(s) 12, 46, 458
Hpy166II GTNNAC 1 cut(s) 526
Hpy188I TCNGA 5 cut(s) 203, 247, 378, 602, 611
Hpy188III TCNNGA 7 cut(s) 15, 163, 368, 389, 413, 438, 486
Hpy8I GTNNAC 1 cut(s) 526
Hpy99I CGWCG 1 cut(s) 106
HpyAV CCTTC 2 cut(s) 94, 220
HpyCH4III ACNGT 3 cut(s) 298, 385, 457
HpyCH4IV ACGT 2 cut(s) 104, 622
HpyCH4V TGCA 1 cut(s) 546
HpyF10VI GCNNNNNNNGC 2 cut(s) 175, 205
HpyF3I CTNAG 3 cut(s) 369, 420, 599
HpySE526I ACGT 2 cut(s) 104, 622
HspAI GCGC 2 cut(s) 126, 616
KflI GGGWCCC 1 cut(s) 140
Kzo9I GATC 2 cut(s) 17, 429
LmnI GCTCC 2 cut(s) 33, 204
LweI GCATC 2 cut(s) 14, 382
MaeI CTAG 1 cut(s) 521
MaeII ACGT 2 cut(s) 104, 622
MaeIII GTNAC 1 cut(s) 105
MalI GATC 2 cut(s) 19, 431
MbiI CCGCTC 2 cut(s) 28, 351
MboI GATC 2 cut(s) 17, 429
MboII GAAGA 7 cut(s) 145, 263, 320, 391, 436, 439, 577
MhlI GDGCHC 1 cut(s) 186
MlyI GAGTC 1 cut(s) 373
MnlI CCTC 9 cut(s) 41, 62, 68, 104, 159, 241, 268, 283, 312
Mph1103I ATGCAT 1 cut(s) 548
MseI TTAA 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 304
MspI CCGG 3 cut(s) 191, 209, 222
MspR9I CCNGG 3 cut(s) 191, 192, 223
MwoI GCNNNNNNNGC 2 cut(s) 175, 205
NciI CCSGG 3 cut(s) 191, 192, 223
NdeII GATC 2 cut(s) 17, 429
NlaIV GGNNCC 4 cut(s) 141, 142, 183, 184
NsiI ATGCAT 1 cut(s) 548
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PfeI GAWTC 3 cut(s) 159, 325, 441
PfoI TCCNGGA 1 cut(s) 221
PkrI GCNGC 1 cut(s) 27
PleI GAGTC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 372
PpuMI RGGWCCY 1 cut(s) 140
Psp5II RGGWCCY 1 cut(s) 140
PspN4I GGNNCC 4 cut(s) 141, 142, 183, 184
PspOMI GGGCCC 1 cut(s) 182
PspPI GGNCC 4 cut(s) 140, 168, 182, 183
PspPPI RGGWCCY 1 cut(s) 140
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
SalI GTCGAC 1 cut(s) 524
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 1 cut(s) 26
Sau3AI GATC 2 cut(s) 17, 429
Sau96I GGNCC 4 cut(s) 140, 168, 182, 183
SchI GAGTC 1 cut(s) 373
ScrFI CCNGG 3 cut(s) 191, 192, 223
SduI GDGCHC 1 cut(s) 186
SetI ASST 7 cut(s) 107, 112, 398, 421, 485, 593, 625
SfaNI GCATC 2 cut(s) 14, 382
SinI GGWCC 1 cut(s) 140
SmaI CCCGGG 1 cut(s) 192
SmlI CTYRAG 1 cut(s) 484
SmoI CTYRAG 1 cut(s) 484
SpeI ACTAGT 1 cut(s) 520
SsiI CCGC 6 cut(s) 23, 26, 37, 118, 302, 351
SspMI CTAG 1 cut(s) 521
StyD4I CCNGG 3 cut(s) 189, 190, 221
TaaI ACNGT 3 cut(s) 298, 385, 457
TaiI ACGT 2 cut(s) 107, 625
TaqI TCGA 4 cut(s) 267, 390, 444, 525
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 3 cut(s) 159, 325, 441
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TspDTI ATGAA 5 cut(s) 320, 398, 487, 522, 583
TspGWI ACGGA 2 cut(s) 393, 528
TspMI CCCGGG 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 140
XmaI CCCGGG 1 cut(s) 190
XmiI GTMKAC 1 cut(s) 525
XspI CTAG 1 cut(s) 521
Zsp2I ATGCAT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.