RchiOBHm_Chr6g0308021

Protein DCL, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010127
Physical Location & Seq
Forward (+)
66169771 .. 66171681
1911 bp
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UTR
Exon/CDS
Intron
PRQ27694

Sequence Viewer

Length: 633 bp
ATGGCCTCTCTCTCCAAACCACCGCTGCTCGTCCACAACCACCTAAGCTCCCTCGCTCTGATTGGTCCCGCAACTCTCTCCTTCCCCATCGCCAAAACGACGTCGCTCCGCACCCGCCTCTGCGCGCTGAAGACCGGGGCCGACGGTGGAAGTAGAACCGGGCGGCAGGAGGCGGACGGGCCGGAGCTGCTGCGGAAGCCGGTGGTGAAGGACGTGGACGTGGATGGGATTTCGGAGGAAGATGAAGGTGAGGATGGGAAATGGGTTGATTGGGAAGATAAGATTCTGGAAGACACGGTTCCGCTGGTTGGGTTTGTGAGGATGATTCTTCATTCTGGGAAATATGAGAGCGGAGATAGGTTGAGTCCCGAGCATGAGAAGACTGTTCTTGAGAGGCTGCTTCCGTTTCATCCTGAAGCTGCAAAGAAGATCGGTTCCGGAATTGATTACGTCACGGTTGGTTATCATCCAGATTTTGAAAGCTCAAGATGTTTGTTCATCGTTCAAAAAGATGGCACATTGGTCGACTTTTCATATTGGAAATGCATAAAGGGGCTGATTAGGAAGAACTATCCCTTATATGCTGACAGCTTCATTCTAAGACATTTTCGAAAGCGTAGACGCGGTGTATGA

Protein Analysis

210

Amino Acids

23.55

Weight (kDa)

7.75

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 107 - 183 7.9e-30 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 104
AccBSI CCGCTC 1 cut(s) 351
AccI GTMKAC 2 cut(s) 525, 619
AccII CGCG 2 cut(s) 125, 624
AccIII TCCGGA 1 cut(s) 437
AcuI CTGAAG 2 cut(s) 149, 435
AcyI GRCGYC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 308
AgsI TTSAA 2 cut(s) 479, 506
AjiI CACGTC 2 cut(s) 214, 220
AluBI AGCT 5 cut(s) 48, 187, 419, 483, 591
AluI AGCT 5 cut(s) 48, 187, 419, 483, 591
Ama87I CYCGRG 1 cut(s) 368
Aor13HI TCCGGA 1 cut(s) 437
AoxI GGCC 3 cut(s) 3, 138, 179
ApeKI GCWGC 5 cut(s) 25, 187, 190, 397, 419
AspLEI GCGC 2 cut(s) 125, 127
AspS9I GGNCC 3 cut(s) 65, 138, 179
AsuC2I CCSGG 2 cut(s) 136, 160
AsuHPI GGTGA 2 cut(s) 217, 260
AsuII TTCGAA 1 cut(s) 610
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 1 cut(s) 65
BbsI GAAGAC 3 cut(s) 137, 297, 386
BbvI GCAGC 5 cut(s) 12, 174, 177, 384, 406
BccI CCATC 4 cut(s) 95, 218, 248, 506
BcgI CGANNNNNNTGC 2 cut(s) 505, 539
BcnI CCSGG 2 cut(s) 136, 160
BisI GCNGC 6 cut(s) 26, 164, 188, 191, 398, 420
BlsI GCNGC 6 cut(s) 27, 165, 189, 192, 399, 421
Bme1390I CCNGG 2 cut(s) 136, 160
Bme18I GGWCC 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 368
BmgBI CACGTC 2 cut(s) 214, 220
BmgT120I GGNCC 3 cut(s) 65, 138, 179
BmiI GGNNCC 4 cut(s) 67, 139, 300, 436
BmrFI CCNGG 2 cut(s) 136, 160
BpiI GAAGAC 3 cut(s) 137, 297, 386
Bpu10I CCTNAGC 1 cut(s) 44
Bpu14I TTCGAA 1 cut(s) 610
BpuEI CTTGAG 2 cut(s) 410, 469
BpuMI CCSGG 2 cut(s) 136, 160
BsaHI GRCGYC 1 cut(s) 101
BsaJI CCNNGG 1 cut(s) 135
BsaWI WCCGGW 1 cut(s) 437
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 1 cut(s) 308
Bse118I RCCGGY 1 cut(s) 199
BseAI TCCGGA 1 cut(s) 437
BseDI CCNNGG 1 cut(s) 135
BseGI GGATG 5 cut(s) 229, 259, 327, 409, 466
BseLI CCNNNNNNNGG 1 cut(s) 308
BsePI GCGCGC 1 cut(s) 123
BseXI GCAGC 5 cut(s) 12, 174, 177, 384, 406
Bsh1236I CGCG 2 cut(s) 125, 624
BshFI GGCC 3 cut(s) 5, 140, 181
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 5 cut(s) 135, 159, 182, 200, 438
BslFI GGGAC 2 cut(s) 51, 351
BslI CCNNNNNNNGG 1 cut(s) 308
BsmFI GGGAC 2 cut(s) 51, 351
BsnI GGCC 3 cut(s) 5, 140, 181
BsoBI CYCGRG 1 cut(s) 368
Bsp119I TTCGAA 1 cut(s) 610
Bsp13I TCCGGA 1 cut(s) 437
Bsp143I GATC 1 cut(s) 429
BspANI GGCC 3 cut(s) 5, 140, 181
BspEI TCCGGA 1 cut(s) 437
BspFNI CGCG 2 cut(s) 125, 624
BspLI GGNNCC 4 cut(s) 67, 139, 300, 436
BspT104I TTCGAA 1 cut(s) 610
BsrBI CCGCTC 1 cut(s) 351
BsrFI RCCGGY 1 cut(s) 199
BssAI RCCGGY 1 cut(s) 199
BssECI CCNNGG 1 cut(s) 135
BssHII GCGCGC 1 cut(s) 123
BssMI GATC 1 cut(s) 429
BssNI GRCGYC 1 cut(s) 101
Bst4CI ACNGT 4 cut(s) 146, 298, 385, 457
BstACI GRCGYC 1 cut(s) 101
BstBI TTCGAA 1 cut(s) 610
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 2 cut(s) 44, 599
BstF5I GGATG 5 cut(s) 229, 259, 327, 409, 466
BstFNI CGCG 2 cut(s) 125, 624
BstHHI GCGC 2 cut(s) 125, 127
BstKTI GATC 1 cut(s) 432
BstMBI GATC 1 cut(s) 429
BstMWI GCNNNNNNNGC 2 cut(s) 187, 196
BstSCI CCNGG 2 cut(s) 134, 158
BstUI CGCG 2 cut(s) 125, 624
BstV1I GCAGC 5 cut(s) 12, 174, 177, 384, 406
BstV2I GAAGAC 3 cut(s) 137, 297, 386
BsuRI GGCC 3 cut(s) 5, 140, 181
BtgZI GCGATG 1 cut(s) 73
BtrI CACGTC 2 cut(s) 214, 220
BtsCI GGATG 5 cut(s) 229, 259, 327, 409, 466
Cac8I GCNNGC 1 cut(s) 125
CfoI GCGC 2 cut(s) 125, 127
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 3 cut(s) 65, 138, 179
CviAII CATG 1 cut(s) 374
DdeI CTNAG 2 cut(s) 44, 599
DpnI GATC 1 cut(s) 431
DpnII GATC 1 cut(s) 429
EciI GGCGGA 1 cut(s) 188
Eco47I GGWCC 1 cut(s) 65
Eco57I CTGAAG 2 cut(s) 149, 435
Eco88I CYCGRG 1 cut(s) 368
EcoT22I ATGCAT 1 cut(s) 548
FaeI CATG 1 cut(s) 377
FaiI YATR 7 cut(s) 345, 375, 535, 548, 580, 582, 631
FaqI GGGAC 2 cut(s) 51, 351
FatI CATG 1 cut(s) 373
FauI CCCGC 2 cut(s) 76, 122
FblI GTMKAC 2 cut(s) 525, 619
Fnu4HI GCNGC 6 cut(s) 26, 164, 188, 191, 398, 420
FokI GGATG 5 cut(s) 236, 266, 334, 396, 453
Fsp4HI GCNGC 6 cut(s) 26, 164, 188, 191, 398, 420
GlaI GCGC 2 cut(s) 124, 126
GluI GCNGC 6 cut(s) 26, 164, 188, 191, 398, 420
HaeIII GGCC 3 cut(s) 5, 140, 181
HapII CCGG 5 cut(s) 135, 159, 182, 200, 438
HhaI GCGC 2 cut(s) 125, 127
Hin1I GRCGYC 1 cut(s) 101
Hin1II CATG 1 cut(s) 377
Hin6I GCGC 2 cut(s) 123, 125
HinP1I GCGC 2 cut(s) 123, 125
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 3 cut(s) 283, 325, 364
HpaII CCGG 5 cut(s) 135, 159, 182, 200, 438
HphI GGTGA 2 cut(s) 217, 260
Hpy166II GTNNAC 4 cut(s) 34, 217, 526, 620
Hpy188I TCNGA 2 cut(s) 60, 235
Hpy188III TCNNGA 7 cut(s) 287, 368, 389, 413, 438, 470, 486
Hpy8I GTNNAC 4 cut(s) 34, 217, 526, 620
Hpy99I CGWCG 3 cut(s) 103, 106, 146
HpyAV CCTTC 3 cut(s) 91, 202, 239
HpyCH4III ACNGT 4 cut(s) 146, 298, 385, 457
HpyCH4IV ACGT 4 cut(s) 101, 213, 219, 450
HpyCH4V TGCA 2 cut(s) 422, 546
HpyF10VI GCNNNNNNNGC 2 cut(s) 187, 196
HpyF3I CTNAG 2 cut(s) 44, 599
HpySE526I ACGT 4 cut(s) 101, 213, 219, 450
Hsp92I GRCGYC 1 cut(s) 101
Hsp92II CATG 1 cut(s) 377
HspAI GCGC 2 cut(s) 123, 125
Kpn2I TCCGGA 1 cut(s) 437
Kzo9I GATC 1 cut(s) 429
LmnI GCTCC 3 cut(s) 53, 111, 184
Lsp1109I GCAGC 5 cut(s) 12, 174, 177, 384, 406
MaeII ACGT 4 cut(s) 101, 213, 219, 450
MaeIII GTNAC 1 cut(s) 451
MalI GATC 1 cut(s) 431
MbiI CCGCTC 1 cut(s) 351
MboI GATC 1 cut(s) 429
MboII GAAGA 8 cut(s) 142, 251, 287, 302, 320, 391, 439, 577
MluCI AATT 1 cut(s) 441
MlyI GAGTC 1 cut(s) 373
MnlI CCTC 8 cut(s) 16, 62, 128, 163, 229, 244, 312, 387
Mph1103I ATGCAT 1 cut(s) 548
MroI TCCGGA 1 cut(s) 437
MspA1I CMGCKG 2 cut(s) 25, 304
MspI CCGG 5 cut(s) 135, 159, 182, 200, 438
MspR9I CCNGG 2 cut(s) 136, 160
MvnI CGCG 2 cut(s) 125, 624
MwoI GCNNNNNNNGC 2 cut(s) 187, 196
NciI CCSGG 2 cut(s) 136, 160
NdeII GATC 1 cut(s) 429
NlaIII CATG 1 cut(s) 377
NlaIV GGNNCC 4 cut(s) 67, 139, 300, 436
NmuCI GTSAC 1 cut(s) 451
NsiI ATGCAT 1 cut(s) 548
NspV TTCGAA 1 cut(s) 610
PauI GCGCGC 1 cut(s) 123
PfeI GAWTC 2 cut(s) 283, 325
PkrI GCNGC 6 cut(s) 27, 165, 189, 192, 399, 421
PleI GAGTC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 372
PspN4I GGNNCC 4 cut(s) 67, 139, 300, 436
PspPI GGNCC 3 cut(s) 65, 138, 179
PteI GCGCGC 1 cut(s) 123
SalI GTCGAC 1 cut(s) 524
SatI GCNGC 6 cut(s) 26, 164, 188, 191, 398, 420
Sau3AI GATC 1 cut(s) 429
Sau96I GGNCC 3 cut(s) 65, 138, 179
SchI GAGTC 1 cut(s) 373
ScrFI CCNGG 2 cut(s) 136, 160
SfuI TTCGAA 1 cut(s) 610
SinI GGWCC 1 cut(s) 65
SmlI CTYRAG 2 cut(s) 389, 484
SmoI CTYRAG 2 cut(s) 389, 484
Sse9I AATT 1 cut(s) 441
StyD4I CCNGG 2 cut(s) 134, 158
TaaI ACNGT 4 cut(s) 146, 298, 385, 457
TaiI ACGT 4 cut(s) 104, 216, 222, 453
TaqI TCGA 2 cut(s) 525, 610
TasI AATT 1 cut(s) 441
TauI GCSGC 1 cut(s) 166
TfiI GAWTC 2 cut(s) 283, 325
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 5 cut(s) 25, 187, 190, 397, 419
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 6 cut(s) 258, 320, 398, 487, 522, 583
TspGWI ACGGA 1 cut(s) 393
VpaK11BI GGWCC 1 cut(s) 65
XmiI GTMKAC 2 cut(s) 525, 619
ZraI GACGTC 1 cut(s) 102
Zsp2I ATGCAT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.