Rw6G041190

Protein DCL, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010127
Physical Location & Seq
Forward (+)
64910645 .. 64912567
1923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G041190.1

Sequence Viewer

Length: 627 bp
ATGGCCTCTCTCTCCAAACCACCGCTGCTCGTCCACAACCACCTAACCTCCCTCGCTCTGATTGGTCCCGCAACTCTCTCCTTCCCCATCGCCAAAACGACGTCGCTCCGCACCCGCCTCTGCGCGCTGAAGACCGGGGCCGACGGCGGAAGTAGAACCGGGCGGCAGGAGGCGGACGGGCCGGAGCTGCTGCGGAAGCCGGTGGTGAAGGACGTGGATGGGATTTCGGAGGAAGATGAAGGTGAGGAAGGGAAATGGGTTGATTGGGAAGATAAGATTCTGGAAGACACGGTTCCGCTGGTTGGGTTTGTGAGGATGATTCTTCATTCTGGGAAATATGAGAGCGGAGATAGGTTGAGTCCCGAGCATGAGAAGACTGTTCTTGAGAGGCTGCTTCCGTTTCATCCTGAAGCTGCAAAGAAGATCGGTTCCGGAATTGATTACGTCACGGTAGGTTATCATCCAGATTTTGAAAGCTCAAGATGTTTGTTCATCGTTCAAAAAGATGGCACATTGGTCGACTTTTCATATTGGAAATGCATTAAGGGGCTGATTAGGAAGAACTATCCCTTATATGCTGACAGCTTCATTCTAAGACATTTTCGAAAGCGTAGACGCGGTGTATGA

Protein Analysis

208

Amino Acids

23.36

Weight (kDa)

8.37

Isoelectric Point (pI)

37.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 105 - 181 7.8e-30 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 104
AccBSI CCGCTC 1 cut(s) 345
AccI GTMKAC 2 cut(s) 519, 613
AccII CGCG 2 cut(s) 125, 618
AccIII TCCGGA 1 cut(s) 431
AcuI CTGAAG 2 cut(s) 149, 429
AcyI GRCGYC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 302
AgsI TTSAA 2 cut(s) 473, 500
AjiI CACGTC 1 cut(s) 214
AluBI AGCT 4 cut(s) 187, 413, 477, 585
AluI AGCT 4 cut(s) 187, 413, 477, 585
Ama87I CYCGRG 1 cut(s) 362
Aor13HI TCCGGA 1 cut(s) 431
AoxI GGCC 3 cut(s) 3, 138, 179
ApeKI GCWGC 5 cut(s) 25, 187, 190, 391, 413
AspLEI GCGC 2 cut(s) 125, 127
AspS9I GGNCC 3 cut(s) 65, 138, 179
AsuC2I CCSGG 2 cut(s) 136, 160
AsuHPI GGTGA 2 cut(s) 217, 254
AsuII TTCGAA 1 cut(s) 604
AvaI CYCGRG 1 cut(s) 362
AvaII GGWCC 1 cut(s) 65
BbsI GAAGAC 3 cut(s) 137, 291, 380
BbvI GCAGC 5 cut(s) 12, 174, 177, 378, 400
BccI CCATC 3 cut(s) 95, 212, 500
BceAI ACGGC 1 cut(s) 160
BcgI CGANNNNNNTGC 2 cut(s) 499, 533
BcnI CCSGG 2 cut(s) 136, 160
BisI GCNGC 6 cut(s) 26, 164, 188, 191, 392, 414
BlsI GCNGC 6 cut(s) 27, 165, 189, 192, 393, 415
Bme1390I CCNGG 2 cut(s) 136, 160
Bme18I GGWCC 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 362
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 3 cut(s) 65, 138, 179
BmiI GGNNCC 4 cut(s) 67, 139, 294, 430
BmrFI CCNGG 2 cut(s) 136, 160
BpiI GAAGAC 3 cut(s) 137, 291, 380
Bpu14I TTCGAA 1 cut(s) 604
BpuEI CTTGAG 2 cut(s) 404, 463
BpuMI CCSGG 2 cut(s) 136, 160
BsaHI GRCGYC 1 cut(s) 101
BsaJI CCNNGG 1 cut(s) 135
BsaWI WCCGGW 1 cut(s) 431
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 1 cut(s) 302
Bse118I RCCGGY 1 cut(s) 199
BseAI TCCGGA 1 cut(s) 431
BseDI CCNNGG 1 cut(s) 135
BseGI GGATG 4 cut(s) 223, 321, 403, 460
BseLI CCNNNNNNNGG 1 cut(s) 302
BsePI GCGCGC 1 cut(s) 123
BseXI GCAGC 5 cut(s) 12, 174, 177, 378, 400
Bsh1236I CGCG 2 cut(s) 125, 618
BshFI GGCC 3 cut(s) 5, 140, 181
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 5 cut(s) 135, 159, 182, 200, 432
BslFI GGGAC 2 cut(s) 51, 345
BslI CCNNNNNNNGG 1 cut(s) 302
BsmFI GGGAC 2 cut(s) 51, 345
BsnI GGCC 3 cut(s) 5, 140, 181
BsoBI CYCGRG 1 cut(s) 362
Bsp119I TTCGAA 1 cut(s) 604
Bsp13I TCCGGA 1 cut(s) 431
Bsp143I GATC 1 cut(s) 423
BspANI GGCC 3 cut(s) 5, 140, 181
BspEI TCCGGA 1 cut(s) 431
BspFNI CGCG 2 cut(s) 125, 618
BspLI GGNNCC 4 cut(s) 67, 139, 294, 430
BspT104I TTCGAA 1 cut(s) 604
BsrBI CCGCTC 1 cut(s) 345
BsrFI RCCGGY 1 cut(s) 199
BssAI RCCGGY 1 cut(s) 199
BssECI CCNNGG 1 cut(s) 135
BssHII GCGCGC 1 cut(s) 123
BssMI GATC 1 cut(s) 423
BssNI GRCGYC 1 cut(s) 101
Bst4CI ACNGT 3 cut(s) 292, 379, 451
BstACI GRCGYC 1 cut(s) 101
BstBI TTCGAA 1 cut(s) 604
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 1 cut(s) 593
BstF5I GGATG 4 cut(s) 223, 321, 403, 460
BstFNI CGCG 2 cut(s) 125, 618
BstHHI GCGC 2 cut(s) 125, 127
BstKTI GATC 1 cut(s) 426
BstMBI GATC 1 cut(s) 423
BstMWI GCNNNNNNNGC 2 cut(s) 187, 196
BstSCI CCNGG 2 cut(s) 134, 158
BstUI CGCG 2 cut(s) 125, 618
BstV1I GCAGC 5 cut(s) 12, 174, 177, 378, 400
BstV2I GAAGAC 3 cut(s) 137, 291, 380
BsuRI GGCC 3 cut(s) 5, 140, 181
BtgZI GCGATG 1 cut(s) 73
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 4 cut(s) 223, 321, 403, 460
Cac8I GCNNGC 1 cut(s) 125
CfoI GCGC 2 cut(s) 125, 127
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 3 cut(s) 65, 138, 179
CviAII CATG 1 cut(s) 368
DdeI CTNAG 1 cut(s) 593
DpnI GATC 1 cut(s) 425
DpnII GATC 1 cut(s) 423
EciI GGCGGA 2 cut(s) 162, 188
Eco47I GGWCC 1 cut(s) 65
Eco57I CTGAAG 2 cut(s) 149, 429
Eco88I CYCGRG 1 cut(s) 362
EcoT22I ATGCAT 1 cut(s) 542
FaeI CATG 1 cut(s) 371
FaiI YATR 6 cut(s) 339, 369, 529, 574, 576, 625
FaqI GGGAC 2 cut(s) 51, 345
FatI CATG 1 cut(s) 367
FauI CCCGC 2 cut(s) 76, 122
FblI GTMKAC 2 cut(s) 519, 613
Fnu4HI GCNGC 6 cut(s) 26, 164, 188, 191, 392, 414
FokI GGATG 4 cut(s) 230, 328, 390, 447
Fsp4HI GCNGC 6 cut(s) 26, 164, 188, 191, 392, 414
GlaI GCGC 2 cut(s) 124, 126
GluI GCNGC 6 cut(s) 26, 164, 188, 191, 392, 414
HaeIII GGCC 3 cut(s) 5, 140, 181
HapII CCGG 5 cut(s) 135, 159, 182, 200, 432
HhaI GCGC 2 cut(s) 125, 127
Hin1I GRCGYC 1 cut(s) 101
Hin1II CATG 1 cut(s) 371
Hin6I GCGC 2 cut(s) 123, 125
HinP1I GCGC 2 cut(s) 123, 125
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HinfI GANTC 3 cut(s) 277, 319, 358
HpaII CCGG 5 cut(s) 135, 159, 182, 200, 432
HphI GGTGA 2 cut(s) 217, 254
Hpy166II GTNNAC 3 cut(s) 34, 520, 614
Hpy188I TCNGA 2 cut(s) 60, 229
Hpy188III TCNNGA 7 cut(s) 281, 362, 383, 407, 432, 464, 480
Hpy8I GTNNAC 3 cut(s) 34, 520, 614
Hpy99I CGWCG 3 cut(s) 103, 106, 146
HpyAV CCTTC 4 cut(s) 91, 202, 233, 242
HpyCH4III ACNGT 3 cut(s) 292, 379, 451
HpyCH4IV ACGT 3 cut(s) 101, 213, 444
HpyCH4V TGCA 2 cut(s) 416, 540
HpyF10VI GCNNNNNNNGC 2 cut(s) 187, 196
HpyF3I CTNAG 1 cut(s) 593
HpySE526I ACGT 3 cut(s) 101, 213, 444
Hsp92I GRCGYC 1 cut(s) 101
Hsp92II CATG 1 cut(s) 371
HspAI GCGC 2 cut(s) 123, 125
Kpn2I TCCGGA 1 cut(s) 431
Kzo9I GATC 1 cut(s) 423
LmnI GCTCC 2 cut(s) 111, 184
Lsp1109I GCAGC 5 cut(s) 12, 174, 177, 378, 400
MaeII ACGT 3 cut(s) 101, 213, 444
MaeIII GTNAC 1 cut(s) 445
MalI GATC 1 cut(s) 425
MbiI CCGCTC 1 cut(s) 345
MboI GATC 1 cut(s) 423
MboII GAAGA 8 cut(s) 142, 245, 281, 296, 314, 385, 433, 571
MluCI AATT 1 cut(s) 435
MlyI GAGTC 1 cut(s) 367
MnlI CCTC 9 cut(s) 16, 58, 62, 128, 163, 223, 238, 306, 381
Mph1103I ATGCAT 1 cut(s) 542
MroI TCCGGA 1 cut(s) 431
MseI TTAA 1 cut(s) 543
MspA1I CMGCKG 2 cut(s) 25, 298
MspI CCGG 5 cut(s) 135, 159, 182, 200, 432
MspR9I CCNGG 2 cut(s) 136, 160
MvnI CGCG 2 cut(s) 125, 618
MwoI GCNNNNNNNGC 2 cut(s) 187, 196
NciI CCSGG 2 cut(s) 136, 160
NdeII GATC 1 cut(s) 423
NlaIII CATG 1 cut(s) 371
NlaIV GGNNCC 4 cut(s) 67, 139, 294, 430
NmuCI GTSAC 1 cut(s) 445
NsiI ATGCAT 1 cut(s) 542
NspV TTCGAA 1 cut(s) 604
PauI GCGCGC 1 cut(s) 123
PfeI GAWTC 2 cut(s) 277, 319
PkrI GCNGC 6 cut(s) 27, 165, 189, 192, 393, 415
PleI GAGTC 1 cut(s) 366
PpsI GAGTC 1 cut(s) 366
PspN4I GGNNCC 4 cut(s) 67, 139, 294, 430
PspPI GGNCC 3 cut(s) 65, 138, 179
PteI GCGCGC 1 cut(s) 123
SalI GTCGAC 1 cut(s) 518
SaqAI TTAA 1 cut(s) 543
SatI GCNGC 6 cut(s) 26, 164, 188, 191, 392, 414
Sau3AI GATC 1 cut(s) 423
Sau96I GGNCC 3 cut(s) 65, 138, 179
SchI GAGTC 1 cut(s) 367
ScrFI CCNGG 2 cut(s) 136, 160
SfuI TTCGAA 1 cut(s) 604
SinI GGWCC 1 cut(s) 65
SmlI CTYRAG 2 cut(s) 383, 478
SmoI CTYRAG 2 cut(s) 383, 478
Sse9I AATT 1 cut(s) 435
StyD4I CCNGG 2 cut(s) 134, 158
TaaI ACNGT 3 cut(s) 292, 379, 451
TaiI ACGT 3 cut(s) 104, 216, 447
TaqI TCGA 2 cut(s) 519, 604
TasI AATT 1 cut(s) 435
TauI GCSGC 1 cut(s) 166
TfiI GAWTC 2 cut(s) 277, 319
Tru1I TTAA 1 cut(s) 543
Tru9I TTAA 1 cut(s) 543
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 5 cut(s) 25, 187, 190, 391, 413
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 6 cut(s) 252, 314, 392, 481, 516, 577
TspGWI ACGGA 1 cut(s) 387
VpaK11BI GGWCC 1 cut(s) 65
XmiI GTMKAC 2 cut(s) 519, 613
ZraI GACGTC 1 cut(s) 102
Zsp2I ATGCAT 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.