Rh6BG482900

Protein DCL, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0010127
Physical Location & Seq
Forward (+)
68817943 .. 68819437
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG482900.1

Sequence Viewer

Length: 645 bp
ATGGCCTCTCTCTCCAAACCACCGCTGCTCGTCCACAACCACCTAAGCTCCCTCGCTCTGATTGGTCCCGCAACTCTATCCTTCCCCATCGCCAAAACGACGTCGCTCCGCACCCGCCTCTGCGCGCTGAAGACCGGCGCCGACGGCGGAAGTAGAACCGGGCGGCAGGAGGCGGACGGGCCGGAGCTGCTGCGGAAGCCGGTGGTGAAGGACTTGGACGTGGACGTGGACGTGGATGGGATTTCGGAGGAAGATGAAGGTGAGGAAGGGAAATGGGTTGATTGGGAAGATAAGATTCTGGAAGACACGGTTCCGCTGGTTGGGTTTGTGAGGATGATTCTTCATTCTGGGAAATATGAGAGCGGAGATAGGTTGAGTCCCGAGCATGAGAAGACTGTTCTTGAGAGGCTGCTTCCGTTTCATCCTGAAGCTGCGAAGAAGATCGGTTCCGGAATTGATTACGTCACGGTTGGTTATCATCCAGATTTTGAAAGCTCAAGATGTTTGTTCATCGTTCAAAAAGATGGTACATTGGTCGACTTTTCATATTGGAAATGCATAAAGGGGCTGATTAGGAAGAACTATCCCTTATATGCTGACAGCTTCATTCTAAGACATTTTCGAAAGCGTAGACGCGGTGTATGA

Protein Analysis

214

Amino Acids

24.0

Weight (kDa)

6.66

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 111 - 187 8.2e-30 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 104
AccB1I GGYRCC 1 cut(s) 137
AccBSI CCGCTC 1 cut(s) 363
AccI GTMKAC 2 cut(s) 537, 631
AccII CGCG 2 cut(s) 125, 636
AccIII TCCGGA 1 cut(s) 449
AcuI CTGAAG 2 cut(s) 149, 447
AcyI GRCGYC 2 cut(s) 101, 138
AfaI GTAC 1 cut(s) 529
AfiI CCNNNNNNNGG 1 cut(s) 320
AgsI TTSAA 2 cut(s) 491, 518
AjiI CACGTC 3 cut(s) 220, 226, 232
AluBI AGCT 5 cut(s) 48, 187, 431, 495, 603
AluI AGCT 5 cut(s) 48, 187, 431, 495, 603
Ama87I CYCGRG 1 cut(s) 380
Aor13HI TCCGGA 1 cut(s) 449
AoxI GGCC 2 cut(s) 3, 179
ApeKI GCWGC 5 cut(s) 25, 187, 190, 409, 431
AspLEI GCGC 3 cut(s) 125, 127, 140
AspS9I GGNCC 2 cut(s) 65, 179
AsuC2I CCSGG 1 cut(s) 160
AsuHPI GGTGA 2 cut(s) 217, 272
AsuII TTCGAA 1 cut(s) 622
AvaI CYCGRG 1 cut(s) 380
AvaII GGWCC 1 cut(s) 65
BaeI ACNNNNGTAYC 2 cut(s) 519, 552
BanI GGYRCC 1 cut(s) 137
BbsI GAAGAC 3 cut(s) 137, 309, 398
BbvI GCAGC 5 cut(s) 12, 174, 177, 396, 418
BccI CCATC 3 cut(s) 95, 230, 518
BceAI ACGGC 1 cut(s) 160
BcnI CCSGG 1 cut(s) 160
BfoI RGCGCY 1 cut(s) 141
BisI GCNGC 6 cut(s) 26, 164, 188, 191, 410, 432
BlsI GCNGC 6 cut(s) 27, 165, 189, 192, 411, 433
Bme1390I CCNGG 1 cut(s) 160
Bme18I GGWCC 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 380
BmgBI CACGTC 3 cut(s) 220, 226, 232
BmgT120I GGNCC 2 cut(s) 65, 179
BmiI GGNNCC 4 cut(s) 67, 139, 312, 448
BmrFI CCNGG 1 cut(s) 160
BpiI GAAGAC 3 cut(s) 137, 309, 398
Bpu10I CCTNAGC 1 cut(s) 44
Bpu14I TTCGAA 1 cut(s) 622
BpuEI CTTGAG 2 cut(s) 422, 481
BpuMI CCSGG 1 cut(s) 160
BsaHI GRCGYC 2 cut(s) 101, 138
BsaWI WCCGGW 1 cut(s) 449
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 1 cut(s) 320
Bse118I RCCGGY 2 cut(s) 134, 199
BseAI TCCGGA 1 cut(s) 449
BseGI GGATG 4 cut(s) 241, 339, 421, 478
BseLI CCNNNNNNNGG 1 cut(s) 320
BsePI GCGCGC 1 cut(s) 123
BseXI GCAGC 5 cut(s) 12, 174, 177, 396, 418
Bsh1236I CGCG 2 cut(s) 125, 636
BshFI GGCC 2 cut(s) 5, 181
BshNI GGYRCC 1 cut(s) 137
BsiHKCI CYCGRG 1 cut(s) 380
BsiSI CCGG 5 cut(s) 135, 159, 182, 200, 450
BslFI GGGAC 2 cut(s) 51, 363
BslI CCNNNNNNNGG 1 cut(s) 320
BsmFI GGGAC 2 cut(s) 51, 363
BsnI GGCC 2 cut(s) 5, 181
BsoBI CYCGRG 1 cut(s) 380
Bsp119I TTCGAA 1 cut(s) 622
Bsp13I TCCGGA 1 cut(s) 449
Bsp143I GATC 1 cut(s) 441
BspANI GGCC 2 cut(s) 5, 181
BspEI TCCGGA 1 cut(s) 449
BspFNI CGCG 2 cut(s) 125, 636
BspLI GGNNCC 4 cut(s) 67, 139, 312, 448
BspT104I TTCGAA 1 cut(s) 622
BspT107I GGYRCC 1 cut(s) 137
BsrBI CCGCTC 1 cut(s) 363
BsrFI RCCGGY 2 cut(s) 134, 199
BssAI RCCGGY 2 cut(s) 134, 199
BssHII GCGCGC 1 cut(s) 123
BssMI GATC 1 cut(s) 441
BssNI GRCGYC 2 cut(s) 101, 138
Bst4CI ACNGT 3 cut(s) 310, 397, 469
BstACI GRCGYC 2 cut(s) 101, 138
BstBI TTCGAA 1 cut(s) 622
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 2 cut(s) 44, 611
BstF5I GGATG 4 cut(s) 241, 339, 421, 478
BstFNI CGCG 2 cut(s) 125, 636
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 3 cut(s) 125, 127, 140
BstKTI GATC 1 cut(s) 444
BstMBI GATC 1 cut(s) 441
BstMWI GCNNNNNNNGC 3 cut(s) 144, 187, 196
BstSCI CCNGG 1 cut(s) 158
BstUI CGCG 2 cut(s) 125, 636
BstV1I GCAGC 5 cut(s) 12, 174, 177, 396, 418
BstV2I GAAGAC 3 cut(s) 137, 309, 398
BsuRI GGCC 2 cut(s) 5, 181
BtgZI GCGATG 1 cut(s) 73
BtrI CACGTC 3 cut(s) 220, 226, 232
BtsCI GGATG 4 cut(s) 241, 339, 421, 478
Cac8I GCNNGC 1 cut(s) 125
CfoI GCGC 3 cut(s) 125, 127, 140
Cfr10I RCCGGY 2 cut(s) 134, 199
Cfr13I GGNCC 2 cut(s) 65, 179
Csp6I GTAC 1 cut(s) 528
CviAII CATG 1 cut(s) 386
CviQI GTAC 1 cut(s) 528
DdeI CTNAG 2 cut(s) 44, 611
DinI GGCGCC 1 cut(s) 139
DpnI GATC 1 cut(s) 443
DpnII GATC 1 cut(s) 441
EciI GGCGGA 2 cut(s) 162, 188
Eco47I GGWCC 1 cut(s) 65
Eco57I CTGAAG 2 cut(s) 149, 447
Eco88I CYCGRG 1 cut(s) 380
EcoT22I ATGCAT 1 cut(s) 560
EgeI GGCGCC 1 cut(s) 139
EheI GGCGCC 1 cut(s) 139
FaeI CATG 1 cut(s) 389
FaiI YATR 7 cut(s) 357, 387, 547, 560, 592, 594, 643
FaqI GGGAC 2 cut(s) 51, 363
FatI CATG 1 cut(s) 385
FauI CCCGC 2 cut(s) 76, 122
FblI GTMKAC 2 cut(s) 537, 631
Fnu4HI GCNGC 6 cut(s) 26, 164, 188, 191, 410, 432
FokI GGATG 4 cut(s) 248, 346, 408, 465
Fsp4HI GCNGC 6 cut(s) 26, 164, 188, 191, 410, 432
GlaI GCGC 3 cut(s) 124, 126, 139
GluI GCNGC 6 cut(s) 26, 164, 188, 191, 410, 432
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 2 cut(s) 5, 181
HapII CCGG 5 cut(s) 135, 159, 182, 200, 450
HhaI GCGC 3 cut(s) 125, 127, 140
Hin1I GRCGYC 2 cut(s) 101, 138
Hin1II CATG 1 cut(s) 389
Hin6I GCGC 3 cut(s) 123, 125, 138
HinP1I GCGC 3 cut(s) 123, 125, 138
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HinfI GANTC 3 cut(s) 295, 337, 376
HpaII CCGG 5 cut(s) 135, 159, 182, 200, 450
HphI GGTGA 2 cut(s) 217, 272
Hpy166II GTNNAC 5 cut(s) 34, 223, 229, 538, 632
Hpy188I TCNGA 2 cut(s) 60, 247
Hpy188III TCNNGA 7 cut(s) 299, 380, 401, 425, 450, 482, 498
Hpy8I GTNNAC 5 cut(s) 34, 223, 229, 538, 632
Hpy99I CGWCG 3 cut(s) 103, 106, 146
HpyAV CCTTC 4 cut(s) 91, 202, 251, 260
HpyCH4III ACNGT 3 cut(s) 310, 397, 469
HpyCH4IV ACGT 5 cut(s) 101, 219, 225, 231, 462
HpyCH4V TGCA 1 cut(s) 558
HpyF10VI GCNNNNNNNGC 3 cut(s) 144, 187, 196
HpyF3I CTNAG 2 cut(s) 44, 611
HpySE526I ACGT 5 cut(s) 101, 219, 225, 231, 462
Hsp92I GRCGYC 2 cut(s) 101, 138
Hsp92II CATG 1 cut(s) 389
HspAI GCGC 3 cut(s) 123, 125, 138
KasI GGCGCC 1 cut(s) 137
Kpn2I TCCGGA 1 cut(s) 449
Kzo9I GATC 1 cut(s) 441
LmnI GCTCC 3 cut(s) 53, 111, 184
Lsp1109I GCAGC 5 cut(s) 12, 174, 177, 396, 418
MaeII ACGT 5 cut(s) 101, 219, 225, 231, 462
MaeIII GTNAC 1 cut(s) 463
MalI GATC 1 cut(s) 443
MbiI CCGCTC 1 cut(s) 363
MboI GATC 1 cut(s) 441
MboII GAAGA 9 cut(s) 142, 263, 299, 314, 332, 403, 448, 451, 589
MluCI AATT 1 cut(s) 453
Mly113I GGCGCC 1 cut(s) 138
MlyI GAGTC 1 cut(s) 385
MnlI CCTC 8 cut(s) 16, 62, 128, 163, 241, 256, 324, 399
Mph1103I ATGCAT 1 cut(s) 560
MroI TCCGGA 1 cut(s) 449
MspA1I CMGCKG 2 cut(s) 25, 316
MspI CCGG 5 cut(s) 135, 159, 182, 200, 450
MspR9I CCNGG 1 cut(s) 160
MvnI CGCG 2 cut(s) 125, 636
MwoI GCNNNNNNNGC 3 cut(s) 144, 187, 196
NarI GGCGCC 1 cut(s) 138
NciI CCSGG 1 cut(s) 160
NdeII GATC 1 cut(s) 441
NlaIII CATG 1 cut(s) 389
NlaIV GGNNCC 4 cut(s) 67, 139, 312, 448
NmuCI GTSAC 1 cut(s) 463
NsiI ATGCAT 1 cut(s) 560
NspV TTCGAA 1 cut(s) 622
PauI GCGCGC 1 cut(s) 123
PfeI GAWTC 2 cut(s) 295, 337
PkrI GCNGC 6 cut(s) 27, 165, 189, 192, 411, 433
PleI GAGTC 1 cut(s) 384
PluTI GGCGCC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 384
PspN4I GGNNCC 4 cut(s) 67, 139, 312, 448
PspPI GGNCC 2 cut(s) 65, 179
PteI GCGCGC 1 cut(s) 123
RsaI GTAC 1 cut(s) 529
RsaNI GTAC 1 cut(s) 528
SalI GTCGAC 1 cut(s) 536
SatI GCNGC 6 cut(s) 26, 164, 188, 191, 410, 432
Sau3AI GATC 1 cut(s) 441
Sau96I GGNCC 2 cut(s) 65, 179
SchI GAGTC 1 cut(s) 385
ScrFI CCNGG 1 cut(s) 160
SfoI GGCGCC 1 cut(s) 139
SfuI TTCGAA 1 cut(s) 622
SinI GGWCC 1 cut(s) 65
SmlI CTYRAG 2 cut(s) 401, 496
SmoI CTYRAG 2 cut(s) 401, 496
Sse9I AATT 1 cut(s) 453
SspDI GGCGCC 1 cut(s) 137
StyD4I CCNGG 1 cut(s) 158
TaaI ACNGT 3 cut(s) 310, 397, 469
TaiI ACGT 5 cut(s) 104, 222, 228, 234, 465
TaqI TCGA 2 cut(s) 537, 622
TasI AATT 1 cut(s) 453
TauI GCSGC 1 cut(s) 166
TfiI GAWTC 2 cut(s) 295, 337
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 5 cut(s) 25, 187, 190, 409, 431
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 6 cut(s) 270, 332, 410, 499, 534, 595
TspGWI ACGGA 1 cut(s) 405
VpaK11BI GGWCC 1 cut(s) 65
XmiI GTMKAC 2 cut(s) 537, 631
ZraI GACGTC 1 cut(s) 102
Zsp2I ATGCAT 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.