MD08G1104000.v1.1

carboxylesterase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011899
Physical Location & Seq
Forward (+)
8796251 .. 8797207
957 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1104000.v1.1.491

Sequence Viewer

Length: 957 bp
ATGGCTGCAACCACCTCACCGGAAGTGTTACTTGACGTCCTTCCATACCTCCGAGTCCTCAAAGATGGCACAATCGACAGACTCGCCGGAACCCAAGTCGCTCCTCCCGGCCTCGATCCACAAACCGGAGTTTTATCCAAAGACATCGTCATCTTCCCCAAGACTGGCGTGTCTGCCCGACTTTACCGACCCAGCACCGCCAAACCCGGCCATAAACTTCCCTTCATCATATACTTCCACGGTGGAGCCTTCTGCATAGCTTCCGCTGGCGAGCCGCTTTACCACACCAGCCTCAACAACCTTGTTGTGGAAGCCAACGCCATAGGGGTTTCAGTAAACTACAGATTAGCACCTGAGCACCCGCTCCCCACCGCTTACGAAGACTGCTGGGCCGCCCTTAACTGGGTTTTCAACGGTGGCACAGGTCGTGATTCATGGGTCAAGGACCACGTCGATTTCGAGCGCGTGTTTTTGGTCGGAGACAGCGCAGGGGCTAACATTGCACACCACTTGGCCTTACGGATCAAGCCCTCCGATCCGGATCCGAAATTGAAGATAGCCGGGATTGGTATGATCAATCCATACTTTTGGGGGAAGGAGCCAATTGGTGGGGAGGTGGGGGATTTCGTGAGGAAGTCCATGGTGGACACGTGGTGGAATTTCGTTTGCCCGTCGGAGGAAGGCTGCGACGACCCGCTTATCAACCCGTTTGTGGGCGGATCGCCGGGGTTGGAGGGGTTGGCTTGTGACAAAATACTTGTTTTGGTGGCAGGAAAAGATATATTGAGGGATAGAGGGATGCTTTACTATGATGAGTTGGTGAAGAGCAAATGGGGAGGGAGGAAGGAGTTGATTGAAACACAAGGGGAGGATCATGTTTTTCATATCTTTAATCCCAATTGTGACAAGGCCAAGATCTTGATTAGGAATTTGGGTAAGTTCATTAATCAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

319

Amino Acids

34.88

Weight (kDa)

5.86

Isoelectric Point (pI)

27.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 62 - 174 2e-12 BD-FAE
COesterase PF00135 62 - 173 9.1e-07 Carboxylesterase family
Abhydrolase_3 PF07859 76 - 295 2.6e-54 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 169
AatII GACGTC 1 cut(s) 39
AccB7I CCANNNNNTGG 1 cut(s) 608
AccBSI CCGCTC 1 cut(s) 364
AccII CGCG 1 cut(s) 465
AccIII TCCGGA 1 cut(s) 538
AciI CCGC 8 cut(s) 198, 264, 275, 362, 372, 393, 695, 717
AclWI GGATC 7 cut(s) 110, 530, 530, 536, 549, 727, 879
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 2 cut(s) 658, 928
AcvI CACGTG 1 cut(s) 651
AcyI GRCGYC 1 cut(s) 36
AdeI CACNNNGTG 1 cut(s) 654
AfiI CCNNNNNNNGG 9 cut(s) 125, 164, 307, 402, 403, 608, 676, 712, 713
AflIII ACRYGT 1 cut(s) 648
AgsI TTSAA 3 cut(s) 412, 553, 857
AjiI CACGTC 1 cut(s) 451
AluBI AGCT 1 cut(s) 260
AluI AGCT 1 cut(s) 260
Alw21I GWGCWC 1 cut(s) 360
Alw26I GTCTC 1 cut(s) 474
AlwI GGATC 7 cut(s) 110, 530, 530, 536, 549, 727, 879
Aor13HI TCCGGA 1 cut(s) 538
AoxI GGCC 5 cut(s) 109, 208, 390, 513, 909
ApeKI GCWGC 2 cut(s) 5, 684
ApoI RAATTY 2 cut(s) 658, 928
ArsI GACNNNNNNTTYG 2 cut(s) 132, 164
AseI ATTAAT 1 cut(s) 945
AspLEI GCGC 2 cut(s) 465, 488
AspS9I GGNCC 2 cut(s) 390, 445
AsuC2I CCSGG 4 cut(s) 108, 207, 562, 726
AsuHPI GGTGA 2 cut(s) 9, 832
AvaII GGWCC 1 cut(s) 445
BamHI GGATCC 1 cut(s) 541
BbrPI CACGTG 1 cut(s) 651
BbsI GAAGAC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 360
BbvI GCAGC 1 cut(s) 671
BccI CCATC 1 cut(s) 59
BclI TGATCA 1 cut(s) 573
BcnI CCSGG 4 cut(s) 108, 207, 562, 726
BcoDI GTCTC 1 cut(s) 474
BfmI CTRYAG 1 cut(s) 340
BglII AGATCT 1 cut(s) 915
BisI GCNGC 4 cut(s) 6, 275, 393, 685
BlsI GCNGC 4 cut(s) 7, 276, 394, 686
Bme1390I CCNGG 4 cut(s) 108, 207, 562, 726
Bme18I GGWCC 1 cut(s) 445
BmgBI CACGTC 1 cut(s) 451
BmgT120I GGNCC 2 cut(s) 390, 445
BmiI GGNNCC 4 cut(s) 91, 247, 543, 600
BmrFI CCNGG 4 cut(s) 108, 207, 562, 726
BmrI ACTGGG 1 cut(s) 412
BmsI GCATC 1 cut(s) 789
BmuI ACTGGG 1 cut(s) 412
BpiI GAAGAC 1 cut(s) 387
Bpu10I CCTNAGC 1 cut(s) 354
BpuMI CCSGG 4 cut(s) 108, 207, 562, 726
BsaAI YACGTR 1 cut(s) 651
BsaBI GATNNNNATC 1 cut(s) 540
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 3 cut(s) 238, 639, 725
BsaWI WCCGGW 3 cut(s) 19, 125, 538
Bsc4I CCNNNNNNNGG 9 cut(s) 125, 164, 307, 402, 403, 608, 676, 712, 713
Bse1I ACTGG 2 cut(s) 169, 407
Bse3DI GCAATG 1 cut(s) 498
Bse8I GATNNNNATC 1 cut(s) 540
BseAI TCCGGA 1 cut(s) 538
BseDI CCNNGG 3 cut(s) 238, 639, 725
BseGI GGATG 1 cut(s) 804
BseJI GATNNNNATC 1 cut(s) 540
BseLI CCNNNNNNNGG 9 cut(s) 125, 164, 307, 402, 403, 608, 676, 712, 713
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 2 cut(s) 169, 407
BseRI GAGGAG 1 cut(s) 93
BseXI GCAGC 1 cut(s) 671
BseYI CCCAGC 2 cut(s) 191, 387
Bsh1236I CGCG 1 cut(s) 465
BshFI GGCC 5 cut(s) 111, 210, 392, 515, 911
BsiHKAI GWGCWC 1 cut(s) 360
BsiSI CCGG 8 cut(s) 20, 87, 108, 126, 207, 539, 561, 725
BslI CCNNNNNNNGG 9 cut(s) 125, 164, 307, 402, 403, 608, 676, 712, 713
BsmAI GTCTC 1 cut(s) 474
BsnI GGCC 5 cut(s) 111, 210, 392, 515, 911
Bsp1286I GDGCHC 1 cut(s) 360
Bsp13I TCCGGA 1 cut(s) 538
Bsp143I GATC 8 cut(s) 115, 522, 535, 541, 573, 719, 871, 915
Bsp19I CCATGG 1 cut(s) 639
BspACI CCGC 8 cut(s) 198, 264, 275, 362, 372, 393, 695, 717
BspANI GGCC 5 cut(s) 111, 210, 392, 515, 911
BspCNI CTCAG 1 cut(s) 346
BspEI TCCGGA 1 cut(s) 538
BspFNI CGCG 1 cut(s) 465
BspLI GGNNCC 4 cut(s) 91, 247, 543, 600
BspPI GGATC 7 cut(s) 110, 530, 530, 536, 549, 727, 879
BspQI GCTCTTC 1 cut(s) 818
BsrBI CCGCTC 1 cut(s) 364
BsrDI GCAATG 1 cut(s) 498
BsrI ACTGG 2 cut(s) 169, 407
BssECI CCNNGG 3 cut(s) 238, 639, 725
BssMI GATC 8 cut(s) 115, 522, 535, 541, 573, 719, 871, 915
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 639
Bst4CI ACNGT 2 cut(s) 242, 416
Bst6I CTCTTC 1 cut(s) 818
BstACI GRCGYC 1 cut(s) 36
BstBAI YACGTR 1 cut(s) 651
BstC8I GCNNGC 2 cut(s) 268, 272
BstDEI CTNAG 1 cut(s) 354
BstDSI CCRYGG 2 cut(s) 238, 639
BstF5I GGATG 1 cut(s) 804
BstFNI CGCG 1 cut(s) 465
BstHHI GCGC 2 cut(s) 465, 488
BstKTI GATC 8 cut(s) 118, 525, 538, 544, 576, 722, 874, 918
BstMAI GTCTC 1 cut(s) 474
BstMBI GATC 8 cut(s) 115, 522, 535, 541, 573, 719, 871, 915
BstMWI GCNNNNNNNGC 1 cut(s) 500
BstSCI CCNGG 4 cut(s) 106, 205, 560, 724
BstSFI CTRYAG 1 cut(s) 340
BstUI CGCG 1 cut(s) 465
BstV1I GCAGC 1 cut(s) 671
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 2 cut(s) 541, 915
BstXI CCANNNNNNTGG 1 cut(s) 588
BstYI RGATCY 2 cut(s) 541, 915
BsuRI GGCC 5 cut(s) 111, 210, 392, 515, 911
BtgI CCRYGG 2 cut(s) 238, 639
BtrI CACGTC 1 cut(s) 451
BtsCI GGATG 1 cut(s) 804
Cac8I GCNNGC 2 cut(s) 268, 272
CfoI GCGC 2 cut(s) 465, 488
Cfr13I GGNCC 2 cut(s) 390, 445
CviAII CATG 3 cut(s) 435, 640, 875
DdeI CTNAG 1 cut(s) 354
DpnI GATC 8 cut(s) 117, 524, 537, 543, 575, 721, 873, 917
DpnII GATC 8 cut(s) 115, 522, 535, 541, 573, 719, 871, 915
DraIII CACNNNGTG 1 cut(s) 654
DrdI GACNNNNNNGTC 1 cut(s) 169
DseDI GACNNNNNNGTC 1 cut(s) 169
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 818
EarI CTCTTC 1 cut(s) 818
EciI GGCGGA 1 cut(s) 732
Eco130I CCWWGG 1 cut(s) 639
Eco47I GGWCC 1 cut(s) 445
Eco72I CACGTG 1 cut(s) 651
EcoT14I CCWWGG 1 cut(s) 639
ErhI CCWWGG 1 cut(s) 639
FaeI CATG 3 cut(s) 438, 643, 878
FalI AAGNNNNNCTT 2 cut(s) 15, 47
FatI CATG 3 cut(s) 434, 639, 874
FauI CCCGC 2 cut(s) 369, 702
FbaI TGATCA 1 cut(s) 573
Fnu4HI GCNGC 4 cut(s) 6, 275, 393, 685
FokI GGATG 1 cut(s) 811
Fsp4HI GCNGC 4 cut(s) 6, 275, 393, 685
GlaI GCGC 2 cut(s) 464, 487
GluI GCNGC 4 cut(s) 6, 275, 393, 685
GsaI CCCAGC 2 cut(s) 195, 391
HaeIII GGCC 5 cut(s) 111, 210, 392, 515, 911
HapII CCGG 8 cut(s) 20, 87, 108, 126, 207, 539, 561, 725
HhaI GCGC 2 cut(s) 465, 488
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 3 cut(s) 438, 643, 878
Hin6I GCGC 2 cut(s) 463, 486
HinP1I GCGC 2 cut(s) 463, 486
HinfI GANTC 3 cut(s) 54, 81, 431
HpaII CCGG 8 cut(s) 20, 87, 108, 126, 207, 539, 561, 725
HphI GGTGA 2 cut(s) 9, 832
Hpy166II GTNNAC 2 cut(s) 337, 646
Hpy188I TCNGA 5 cut(s) 53, 479, 535, 546, 676
Hpy188III TCNNGA 5 cut(s) 428, 539, 628, 919, 950
Hpy8I GTNNAC 2 cut(s) 337, 646
Hpy99I CGWCG 3 cut(s) 455, 676, 692
HpyAV CCTTC 6 cut(s) 50, 232, 259, 589, 674, 838
HpyCH4III ACNGT 2 cut(s) 242, 416
HpyCH4IV ACGT 3 cut(s) 36, 450, 650
HpyCH4V TGCA 3 cut(s) 8, 255, 503
HpyF10VI GCNNNNNNNGC 1 cut(s) 500
HpyF3I CTNAG 1 cut(s) 354
HpySE526I ACGT 3 cut(s) 36, 450, 650
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 3 cut(s) 438, 643, 878
HspAI GCGC 2 cut(s) 463, 486
Kpn2I TCCGGA 1 cut(s) 538
Ksp22I TGATCA 1 cut(s) 573
Kzo9I GATC 8 cut(s) 115, 522, 535, 541, 573, 719, 871, 915
LguI GCTCTTC 1 cut(s) 818
LmnI GCTCC 4 cut(s) 106, 245, 369, 598
Lsp1109I GCAGC 1 cut(s) 671
LweI GCATC 1 cut(s) 789
MaeII ACGT 3 cut(s) 36, 450, 650
MaeIII GTNAC 3 cut(s) 27, 746, 902
MalI GATC 8 cut(s) 117, 524, 537, 543, 575, 721, 873, 917
MbiI CCGCTC 1 cut(s) 364
MboI GATC 8 cut(s) 115, 522, 535, 541, 573, 719, 871, 915
MboII GAAGA 4 cut(s) 145, 392, 565, 835
MfeI CAATTG 2 cut(s) 603, 898
MflI RGATCY 2 cut(s) 541, 915
MhlI GDGCHC 1 cut(s) 360
MluCI AATT 5 cut(s) 548, 603, 658, 898, 928
MlyI GAGTC 2 cut(s) 63, 75
MmeI TCCRAC 3 cut(s) 457, 654, 711
MroI TCCGGA 1 cut(s) 538
MseI TTAA 3 cut(s) 399, 891, 945
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 8 cut(s) 20, 87, 108, 126, 207, 539, 561, 725
MspR9I CCNGG 4 cut(s) 108, 207, 562, 726
MunI CAATTG 2 cut(s) 603, 898
MvnI CGCG 1 cut(s) 465
MwoI GCNNNNNNNGC 1 cut(s) 500
NciI CCSGG 4 cut(s) 108, 207, 562, 726
NcoI CCATGG 1 cut(s) 639
NdeII GATC 8 cut(s) 115, 522, 535, 541, 573, 719, 871, 915
NlaIII CATG 3 cut(s) 438, 643, 878
NlaIV GGNNCC 4 cut(s) 91, 247, 543, 600
NmuCI GTSAC 2 cut(s) 746, 902
PciSI GCTCTTC 1 cut(s) 818
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PfeI GAWTC 1 cut(s) 431
PflFI GACNNNGTC 2 cut(s) 146, 449
PflMI CCANNNNNTGG 1 cut(s) 608
PkrI GCNGC 4 cut(s) 7, 276, 394, 686
PleI GAGTC 2 cut(s) 62, 75
PmaCI CACGTG 1 cut(s) 651
PmlI CACGTG 1 cut(s) 651
PpsI GAGTC 2 cut(s) 62, 75
Ppu21I YACGTR 1 cut(s) 651
PshBI ATTAAT 1 cut(s) 945
PspCI CACGTG 1 cut(s) 651
PspFI CCCAGC 2 cut(s) 191, 387
PspN4I GGNNCC 4 cut(s) 91, 247, 543, 600
PspPI GGNCC 2 cut(s) 390, 445
PsuI RGATCY 2 cut(s) 541, 915
PsyI GACNNNGTC 2 cut(s) 146, 449
SapI GCTCTTC 1 cut(s) 818
SaqAI TTAA 3 cut(s) 399, 891, 945
SatI GCNGC 4 cut(s) 6, 275, 393, 685
Sau3AI GATC 8 cut(s) 115, 522, 535, 541, 573, 719, 871, 915
Sau96I GGNCC 2 cut(s) 390, 445
SchI GAGTC 2 cut(s) 63, 75
ScrFI CCNGG 4 cut(s) 108, 207, 562, 726
SduI GDGCHC 1 cut(s) 360
SfaNI GCATC 1 cut(s) 789
SfcI CTRYAG 1 cut(s) 340
SinI GGWCC 1 cut(s) 445
Sse9I AATT 5 cut(s) 548, 603, 658, 898, 928
SsiI CCGC 8 cut(s) 198, 264, 275, 362, 372, 393, 695, 717
StyD4I CCNGG 4 cut(s) 106, 205, 560, 724
StyI CCWWGG 1 cut(s) 639
TaaI ACNGT 2 cut(s) 242, 416
TaiI ACGT 3 cut(s) 39, 453, 653
TaqI TCGA 4 cut(s) 75, 114, 453, 459
TasI AATT 5 cut(s) 548, 603, 658, 898, 928
TauI GCSGC 2 cut(s) 277, 395
TfiI GAWTC 1 cut(s) 431
Tru1I TTAA 3 cut(s) 399, 891, 945
Tru9I TTAA 3 cut(s) 399, 891, 945
TseFI GTSAC 2 cut(s) 746, 902
TseI GCWGC 2 cut(s) 5, 684
Tsp45I GTSAC 2 cut(s) 746, 902
TspDTI ATGAA 4 cut(s) 214, 423, 872, 931
TspGWI ACGGA 1 cut(s) 535
Tth111I GACNNNGTC 2 cut(s) 146, 449
Van91I CCANNNNNTGG 1 cut(s) 608
VpaK11BI GGWCC 1 cut(s) 445
VspI ATTAAT 1 cut(s) 945
XapI RAATTY 2 cut(s) 658, 928
ZraI GACGTC 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.