RLG00000010716

carboxylesterase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0011899
Physical Location & Seq
Forward (+)
3819744 .. 3820688
945 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010716

Sequence Viewer

Length: 945 bp
ATGGGTTCCAGTGAAGTCTCTCTTGAAGTTCCTCCATACGCGCGAGTACTCAAAGATGGAACAATTGAGAGACTTGCCGGAACCCAAGTTGTTCCGGCCGGGTTAGACCCTCAAACCGGGGTTTTGTCCAAAGACATCGTGATTTTCCCACAAACCGGTGTCTCAGCTAGACTTTACCGACCAAATATCACCGCTACGGACCAAAAGCTTCCTTTGATCGTTTACTATCATGGTGGAGCTTTTTGCATAGCTTCAGCTGCCGACCCACTCTACCACAACTGCCTTAACATGCTTGTTGCTGAGGCCAGAGCCATTGCTGTTTCTGTTAATTACAGATTAGCCCCTGAGCATCCACTGCCGATTGCCTACGAGGATTCTTGGGCAGTGCTTCAATGGGTTTTTGGTGGTGAAGATCGTGATGATTGGGTTAAGGATCATGTTGATTTTGACCGAGTGTTCTTGGTGGGAGATAGTGCTGGGGCTAATATTGCACACCACTTGGCCCTGCGGGTCAAGAAGTCCGACCCGGATCCGAAGCTGAAGATTGCTGGGATTGGGCTGATCCATCCTTATTTCTGGGGGAAAGATCCGATTGGCCGGGAGGTGGCGGATTCGTTCAGGAAGTCAATGGTGGACACGTGGTGGAATTTCGTCTGCCCATCGGAGAAAGAGGGCGATGACCCGCTTATCAACCCGTTTCTTGACGGAGCCCCGGGTCTCGAGGGGTTGGCTTGTGGCAAGGTGCTTGTTCTTGTTGCAGGAAATGACATATTGAGGGACAGAGGGAGGCTTTATTATGATGAATTGGTGAAGAGCAACTGGGCAGGAAGCAAAGAGTTCATTGAAACAGAAGGGGAAGACCATGTGTTCCATATCTTCAATCCCAATTGTGAAAAGGCCAAGAGCTTGATCAAGCATTTGGGTTCTTTCATAAACCAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

315

Amino Acids

34.69

Weight (kDa)

5.37

Isoelectric Point (pI)

26.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 65 - 173 4.4e-11 BD-FAE
Abhydrolase_3 PF07859 73 - 291 6.3e-54 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 41, 43
AciI CCGC 4 cut(s) 192, 508, 608, 683
AclWI GGATC 5 cut(s) 441, 524, 537, 556, 581
AcoI YGGCCR 2 cut(s) 96, 595
AcsI RAATTY 1 cut(s) 646
AcuI CTGAAG 2 cut(s) 237, 560
AcvI CACGTG 1 cut(s) 639
AdeI CACNNNGTG 1 cut(s) 642
AfaI GTAC 1 cut(s) 48
AfiI CCNNNNNNNGG 3 cut(s) 116, 155, 604
AflIII ACRYGT 1 cut(s) 636
AgeI ACCGGT 1 cut(s) 155
AgsI TTSAA 4 cut(s) 26, 392, 845, 880
AluBI AGCT 7 cut(s) 167, 208, 239, 251, 257, 538, 906
AluI AGCT 7 cut(s) 167, 208, 239, 251, 257, 538, 906
Alw26I GTCTC 4 cut(s) 22, 64, 166, 722
AlwI GGATC 5 cut(s) 441, 524, 537, 556, 581
Ama87I CYCGRG 2 cut(s) 712, 719
AoxI GGCC 5 cut(s) 96, 303, 501, 595, 897
ApeKI GCWGC 1 cut(s) 257
ApoI RAATTY 1 cut(s) 646
AsiGI ACCGGT 1 cut(s) 155
AspLEI GCGC 1 cut(s) 43
AspS9I GGNCC 2 cut(s) 199, 502
AsuC2I CCSGG 6 cut(s) 100, 118, 527, 599, 713, 714
AsuHPI GGTGA 3 cut(s) 181, 419, 820
AvaI CYCGRG 2 cut(s) 712, 719
AvaII GGWCC 1 cut(s) 199
BamHI GGATCC 1 cut(s) 529
BanII GRGCYC 1 cut(s) 712
BbrPI CACGTG 1 cut(s) 639
BbsI GAAGAC 1 cut(s) 864
BbvCI CCTCAGC 1 cut(s) 300
BbvI GCAGC 1 cut(s) 244
BccI CCATC 3 cut(s) 50, 573, 667
BclI TGATCA 1 cut(s) 909
BcnI CCSGG 6 cut(s) 100, 118, 527, 599, 713, 714
BcoDI GTCTC 4 cut(s) 22, 64, 166, 722
BfaI CTAG 2 cut(s) 168, 943
BisI GCNGC 1 cut(s) 258
BlsI GCNGC 1 cut(s) 259
BmcAI AGTACT 1 cut(s) 48
Bme1390I CCNGG 6 cut(s) 100, 118, 527, 599, 713, 714
Bme18I GGWCC 1 cut(s) 199
BmeT110I CYCGRG 2 cut(s) 712, 719
BmgT120I GGNCC 2 cut(s) 199, 502
BmiI GGNNCC 4 cut(s) 7, 82, 531, 709
BmrFI CCNGG 6 cut(s) 100, 118, 527, 599, 713, 714
BmrI ACTGGG 1 cut(s) 829
BmsI GCATC 1 cut(s) 358
BmuI ACTGGG 1 cut(s) 829
BpiI GAAGAC 1 cut(s) 864
Bpu10I CCTNAGC 2 cut(s) 300, 345
BpuMI CCSGG 6 cut(s) 100, 118, 527, 599, 713, 714
BsaAI YACGTR 1 cut(s) 639
BsaI GGTCTC 1 cut(s) 722
BsaJI CCNNGG 3 cut(s) 117, 711, 712
BsaWI WCCGGW 1 cut(s) 155
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 155, 604
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 2 cut(s) 9, 824
Bse3DI GCAATG 1 cut(s) 312
BseDI CCNNGG 3 cut(s) 117, 711, 712
BseGI GGATG 2 cut(s) 349, 565
BseLI CCNNNNNNNGG 3 cut(s) 116, 155, 604
BseMI GCAATG 1 cut(s) 312
BseMII CTCAG 3 cut(s) 177, 291, 336
BseNI ACTGG 2 cut(s) 9, 824
BseX3I CGGCCG 1 cut(s) 96
BseXI GCAGC 1 cut(s) 244
BseYI CCCAGC 2 cut(s) 476, 548
Bsh1236I CGCG 2 cut(s) 41, 43
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 5 cut(s) 98, 305, 503, 597, 899
BshTI ACCGGT 1 cut(s) 155
BsiEI CGRYCG 1 cut(s) 99
BsiHKCI CYCGRG 2 cut(s) 712, 719
BsiSI CCGG 8 cut(s) 78, 95, 99, 117, 156, 527, 598, 713
BslFI GGGAC 1 cut(s) 791
BslI CCNNNNNNNGG 3 cut(s) 116, 155, 604
BsmAI GTCTC 4 cut(s) 22, 64, 166, 722
BsmFI GGGAC 1 cut(s) 791
BsnI GGCC 5 cut(s) 98, 305, 503, 597, 899
Bso31I GGTCTC 1 cut(s) 722
BsoBI CYCGRG 2 cut(s) 712, 719
Bsp1286I GDGCHC 1 cut(s) 712
Bsp143I GATC 7 cut(s) 216, 412, 433, 529, 561, 586, 909
BspACI CCGC 4 cut(s) 192, 508, 608, 683
BspANI GGCC 5 cut(s) 98, 305, 503, 597, 899
BspCNI CTCAG 3 cut(s) 176, 292, 337
BspFNI CGCG 2 cut(s) 41, 43
BspLI GGNNCC 4 cut(s) 7, 82, 531, 709
BspPI GGATC 5 cut(s) 441, 524, 537, 556, 581
BspQI GCTCTTC 1 cut(s) 806
BspTNI GGTCTC 1 cut(s) 722
BsrDI GCAATG 1 cut(s) 312
BsrFI RCCGGY 1 cut(s) 155
BsrI ACTGG 2 cut(s) 9, 824
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 3 cut(s) 117, 711, 712
BssMI GATC 7 cut(s) 216, 412, 433, 529, 561, 586, 909
Bst6I CTCTTC 1 cut(s) 806
BstAPI GCANNNNNTGC 1 cut(s) 355
BstBAI YACGTR 1 cut(s) 639
BstDEI CTNAG 3 cut(s) 163, 300, 345
BstF5I GGATG 2 cut(s) 349, 565
BstFNI CGCG 2 cut(s) 41, 43
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 7 cut(s) 219, 415, 436, 532, 564, 589, 912
BstMAI GTCTC 4 cut(s) 22, 64, 166, 722
BstMBI GATC 7 cut(s) 216, 412, 433, 529, 561, 586, 909
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 3 cut(s) 257, 355, 488
BstNSI RCATGY 1 cut(s) 292
BstSCI CCNGG 6 cut(s) 98, 116, 525, 597, 711, 712
BstUI CGCG 2 cut(s) 41, 43
BstV1I GCAGC 1 cut(s) 244
BstV2I GAAGAC 1 cut(s) 864
BstX2I RGATCY 2 cut(s) 529, 586
BstYI RGATCY 2 cut(s) 529, 586
BstZI CGGCCG 1 cut(s) 96
BsuRI GGCC 5 cut(s) 98, 305, 503, 597, 899
BtgZI GCGATG 1 cut(s) 690
BtsCI GGATG 2 cut(s) 349, 565
BtsI GCAGTG 2 cut(s) 353, 390
BtsIMutI CAGTG 3 cut(s) 16, 353, 390
CfoI GCGC 1 cut(s) 43
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 2 cut(s) 199, 502
Cfr9I CCCGGG 1 cut(s) 712
Csp6I GTAC 1 cut(s) 47
CspAI ACCGGT 1 cut(s) 155
CviAII CATG 4 cut(s) 230, 289, 437, 863
CviQI GTAC 1 cut(s) 47
DdeI CTNAG 3 cut(s) 163, 300, 345
DpnI GATC 7 cut(s) 218, 414, 435, 531, 563, 588, 911
DpnII GATC 7 cut(s) 216, 412, 433, 529, 561, 586, 909
DraIII CACNNNGTG 1 cut(s) 642
EaeI YGGCCR 2 cut(s) 96, 595
EagI CGGCCG 1 cut(s) 96
Eam1104I CTCTTC 1 cut(s) 806
EarI CTCTTC 1 cut(s) 806
EciI GGCGGA 1 cut(s) 623
EclXI CGGCCG 1 cut(s) 96
Eco24I GRGCYC 1 cut(s) 712
Eco31I GGTCTC 1 cut(s) 722
Eco47I GGWCC 1 cut(s) 199
Eco52I CGGCCG 1 cut(s) 96
Eco57I CTGAAG 2 cut(s) 237, 560
Eco72I CACGTG 1 cut(s) 639
Eco88I CYCGRG 2 cut(s) 712, 719
EcoT38I GRGCYC 1 cut(s) 712
FaeI CATG 4 cut(s) 233, 292, 440, 866
FalI AAGNNNNNCTT 1 cut(s) 38
FaqI GGGAC 1 cut(s) 791
FatI CATG 4 cut(s) 229, 288, 436, 862
FauI CCCGC 2 cut(s) 501, 690
FbaI TGATCA 1 cut(s) 909
Fnu4HI GCNGC 1 cut(s) 258
FokI GGATG 2 cut(s) 336, 552
FriOI GRGCYC 1 cut(s) 712
Fsp4HI GCNGC 1 cut(s) 258
FspBI CTAG 2 cut(s) 168, 943
GlaI GCGC 1 cut(s) 42
GluI GCNGC 1 cut(s) 258
GsaI CCCAGC 2 cut(s) 480, 552
HaeIII GGCC 5 cut(s) 98, 305, 503, 597, 899
HapII CCGG 8 cut(s) 78, 95, 99, 117, 156, 527, 598, 713
HhaI GCGC 1 cut(s) 43
Hin1II CATG 4 cut(s) 233, 292, 440, 866
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 2 cut(s) 374, 611
HpaII CCGG 8 cut(s) 78, 95, 99, 117, 156, 527, 598, 713
HphI GGTGA 3 cut(s) 181, 419, 820
Hpy166II GTNNAC 2 cut(s) 223, 634
Hpy188I TCNGA 4 cut(s) 523, 534, 591, 664
Hpy188III TCNNGA 7 cut(s) 23, 139, 416, 514, 619, 701, 719
Hpy8I GTNNAC 2 cut(s) 223, 634
HpyAV CCTTC 1 cut(s) 845
HpyCH4IV ACGT 1 cut(s) 638
HpyCH4V TGCA 3 cut(s) 246, 491, 758
HpyF10VI GCNNNNNNNGC 3 cut(s) 257, 355, 488
HpyF3I CTNAG 3 cut(s) 163, 300, 345
HpySE526I ACGT 1 cut(s) 638
Hsp92II CATG 4 cut(s) 233, 292, 440, 866
HspAI GCGC 1 cut(s) 41
Ksp22I TGATCA 1 cut(s) 909
Kzo9I GATC 7 cut(s) 216, 412, 433, 529, 561, 586, 909
LguI GCTCTTC 1 cut(s) 806
LmnI GCTCC 2 cut(s) 236, 707
Lsp1109I GCAGC 1 cut(s) 244
LweI GCATC 1 cut(s) 358
MaeI CTAG 2 cut(s) 168, 943
MaeII ACGT 1 cut(s) 638
MalI GATC 7 cut(s) 218, 414, 435, 531, 563, 588, 911
MboI GATC 7 cut(s) 216, 412, 433, 529, 561, 586, 909
MboII GAAGA 5 cut(s) 422, 553, 823, 868, 869
MfeI CAATTG 2 cut(s) 63, 886
MflI RGATCY 2 cut(s) 529, 586
MhlI GDGCHC 1 cut(s) 712
MluCI AATT 5 cut(s) 63, 328, 646, 803, 886
MmeI TCCRAC 1 cut(s) 546
MseI TTAA 3 cut(s) 285, 327, 429
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 8 cut(s) 78, 95, 99, 117, 156, 527, 598, 713
MspR9I CCNGG 6 cut(s) 100, 118, 527, 599, 713, 714
MunI CAATTG 2 cut(s) 63, 886
MvnI CGCG 2 cut(s) 41, 43
MwoI GCNNNNNNNGC 3 cut(s) 257, 355, 488
NciI CCSGG 6 cut(s) 100, 118, 527, 599, 713, 714
NdeII GATC 7 cut(s) 216, 412, 433, 529, 561, 586, 909
NlaIII CATG 4 cut(s) 233, 292, 440, 866
NlaIV GGNNCC 4 cut(s) 7, 82, 531, 709
NspI RCATGY 1 cut(s) 292
PaeR7I CTCGAG 1 cut(s) 719
PciSI GCTCTTC 1 cut(s) 806
PfeI GAWTC 2 cut(s) 374, 611
PinAI ACCGGT 1 cut(s) 155
PkrI GCNGC 1 cut(s) 259
PmaCI CACGTG 1 cut(s) 639
PmlI CACGTG 1 cut(s) 639
Ppu21I YACGTR 1 cut(s) 639
PspCI CACGTG 1 cut(s) 639
PspFI CCCAGC 2 cut(s) 476, 548
PspN4I GGNNCC 4 cut(s) 7, 82, 531, 709
PspPI GGNCC 2 cut(s) 199, 502
PsuI RGATCY 2 cut(s) 529, 586
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
SapI GCTCTTC 1 cut(s) 806
SaqAI TTAA 3 cut(s) 285, 327, 429
SatI GCNGC 1 cut(s) 258
Sau3AI GATC 7 cut(s) 216, 412, 433, 529, 561, 586, 909
Sau96I GGNCC 2 cut(s) 199, 502
ScaI AGTACT 1 cut(s) 48
ScrFI CCNGG 6 cut(s) 100, 118, 527, 599, 713, 714
SduI GDGCHC 1 cut(s) 712
SfaNI GCATC 1 cut(s) 358
Sfr274I CTCGAG 1 cut(s) 719
SinI GGWCC 1 cut(s) 199
SlaI CTCGAG 1 cut(s) 719
SmaI CCCGGG 1 cut(s) 714
SmlI CTYRAG 1 cut(s) 719
SmoI CTYRAG 1 cut(s) 719
Sse9I AATT 5 cut(s) 63, 328, 646, 803, 886
SsiI CCGC 4 cut(s) 192, 508, 608, 683
SspI AATATT 1 cut(s) 487
SspMI CTAG 2 cut(s) 168, 943
StyD4I CCNGG 6 cut(s) 98, 116, 525, 597, 711, 712
TaiI ACGT 1 cut(s) 641
TaqI TCGA 1 cut(s) 720
TaqII GACCGA 1 cut(s) 465
TasI AATT 5 cut(s) 63, 328, 646, 803, 886
TatI WGTACW 1 cut(s) 46
TfiI GAWTC 2 cut(s) 374, 611
Tru1I TTAA 3 cut(s) 285, 327, 429
Tru9I TTAA 3 cut(s) 285, 327, 429
TscAI CASTG 3 cut(s) 16, 360, 390
TseI GCWGC 1 cut(s) 257
TspDTI ATGAA 3 cut(s) 816, 829, 919
TspGWI ACGGA 2 cut(s) 212, 720
TspMI CCCGGG 1 cut(s) 712
TspRI CASTG 3 cut(s) 16, 360, 390
VpaK11BI GGWCC 1 cut(s) 199
XapI RAATTY 1 cut(s) 646
XceI RCATGY 1 cut(s) 292
XhoI CTCGAG 1 cut(s) 719
XmaI CCCGGG 1 cut(s) 712
XspI CTAG 2 cut(s) 168, 943
ZrmI AGTACT 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.