MD15G1085200.v1.1

carboxylesterase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011899
Physical Location & Seq
Forward (+)
5868197 .. 5869153
957 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1085200.v1.1.491

Sequence Viewer

Length: 957 bp
ATGGCTGCAACCACCTCACCAGAAGTGTTACTCGAGGTCTTTCCATACCTCCGGGTCCTCAAAGACGGCACAATCGACAGACTCGCCGGAACCCAAGTCGCTCCTCCCGGCTTAGACCCCGAAACCGGAGTTTTATCCAAAGACATCGTCATCTTACCCCAAACCGGCGTCTCAGCCCGACTCTACCGACCCATCACCGCCAAACCTGGCACAAAACTCCCCCTAGTCGTATACCTCCACGGTGGCGCCTTCTGCATATCCTCAGCCGCCGACCCGTGTTACCACACCAGCCTCAACAACCTTGTTGCAGAAGCCAACGCCATCGCAGTTTCAGTAAATTACAGATTAGCCCCTGAGTACCCGCTCCCCGCCGCCTATGAAGACTGCTGGGCCGCCCTCAACTGGGTTTTTAACTGCGGCGAAGATCGTGATTCGTGGGTCAAGGATGACGTCGATTTCGGGCGCGTGTTTTTGGTCGGAGACAGCGCAGGAGCCAACATTGCACACCACTTGGCCTTACGGATCAAGGACTCCGACCCGGATCCGAAGTTGAAAATTGCCGGGATTGGTATGGTTAATCCTTACTTTTGGGGGAAGGAGCCAATTGGCGGGGAGGTGGGGGATTTGGTGAGGAAGTCAATGGTGGACACGTGGTGGAATTTCGTTTGTCCGTCGGAAAAAGGCGGCGATGACCCGCTTATCAACCCGTTTCTGGACGGAGCGCCGGGGTTGGAGGGGTTGGCTTGTGGGAAAGTGCTTGTTATGGTGGCAGAAAAAGACATATTGAGGGACAGAGGGAGGCTTTACTATGAGGAATTGGTGAAGAGCAAATGGGGAGGGAGGAAGGAGTTGATTGAAACACAAGGGGAGGATCATGATTTTCATATCTTTAATCCCAATTGCGACAAGGCTAAGATCTTGATTAGGGATTTGGGTAAGTTCATTAATCAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

319

Amino Acids

34.86

Weight (kDa)

5.09

Isoelectric Point (pI)

24.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 62 - 172 1.5e-07 Carboxylesterase family
BD-FAE PF20434 68 - 176 7.1e-14 BD-FAE
Abhydrolase_3 PF07859 76 - 295 6.6e-55 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 453
AccB1I GGYRCC 1 cut(s) 245
AccBSI CCGCTC 1 cut(s) 364
AccI GTMKAC 1 cut(s) 231
AccII CGCG 1 cut(s) 465
AclWI GGATC 4 cut(s) 530, 536, 549, 879
AcsI RAATTY 1 cut(s) 658
AcvI CACGTG 1 cut(s) 651
AcyI GRCGYC 3 cut(s) 168, 246, 450
AdeI CACNNNGTG 1 cut(s) 654
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 6 cut(s) 125, 164, 402, 403, 608, 712
AflIII ACRYGT 1 cut(s) 648
AgsI TTSAA 2 cut(s) 553, 857
AjnI CCWGG 1 cut(s) 205
Alw26I GTCTC 2 cut(s) 175, 474
AlwI GGATC 4 cut(s) 530, 536, 549, 879
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 2 cut(s) 390, 513
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 658
ArsI GACNNNNNNTTYG 4 cut(s) 132, 164, 440, 472
AseI ATTAAT 1 cut(s) 945
AspLEI GCGC 4 cut(s) 248, 465, 488, 724
AspS9I GGNCC 2 cut(s) 55, 390
AsuC2I CCSGG 5 cut(s) 53, 108, 539, 562, 726
AsuHPI GGTGA 4 cut(s) 9, 187, 640, 832
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 1 cut(s) 55
BamHI GGATCC 1 cut(s) 541
BanI GGYRCC 1 cut(s) 245
BbrPI CACGTG 1 cut(s) 651
BbsI GAAGAC 1 cut(s) 387
BbvCI CCTCAGC 1 cut(s) 262
BccI CCATC 2 cut(s) 200, 329
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 1 cut(s) 207
BcnI CCSGG 5 cut(s) 53, 108, 539, 562, 726
BcoDI GTCTC 2 cut(s) 175, 474
BfaI CTAG 1 cut(s) 224
BfoI RGCGCY 2 cut(s) 249, 725
BglII AGATCT 1 cut(s) 915
BisI GCNGC 6 cut(s) 6, 267, 372, 393, 418, 685
BlsI GCNGC 6 cut(s) 7, 268, 373, 394, 419, 686
Bme1390I CCNGG 6 cut(s) 53, 108, 207, 539, 562, 726
Bme18I GGWCC 1 cut(s) 55
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 2 cut(s) 55, 390
BmiI GGNNCC 6 cut(s) 56, 91, 247, 493, 543, 600
BmrFI CCNGG 6 cut(s) 53, 108, 207, 539, 562, 726
BmrI ACTGGG 1 cut(s) 412
BmuI ACTGGG 1 cut(s) 412
BpiI GAAGAC 1 cut(s) 387
Bpu10I CCTNAGC 1 cut(s) 262
BpuMI CCSGG 5 cut(s) 53, 108, 539, 562, 726
BsaAI YACGTR 1 cut(s) 651
BsaHI GRCGYC 3 cut(s) 168, 246, 450
BsaJI CCNNGG 2 cut(s) 238, 725
BsaWI WCCGGW 1 cut(s) 125
Bsc4I CCNNNNNNNGG 6 cut(s) 125, 164, 402, 403, 608, 712
Bse118I RCCGGY 1 cut(s) 164
Bse1I ACTGG 1 cut(s) 407
Bse3DI GCAATG 1 cut(s) 498
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 238, 725
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 6 cut(s) 125, 164, 402, 403, 608, 712
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 3 cut(s) 186, 276, 345
BseNI ACTGG 1 cut(s) 407
BseRI GAGGAG 1 cut(s) 93
BseYI CCCAGC 1 cut(s) 387
Bsh1236I CGCG 1 cut(s) 465
BshFI GGCC 2 cut(s) 392, 515
BshNI GGYRCC 1 cut(s) 245
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 8 cut(s) 52, 87, 108, 126, 165, 539, 561, 725
BslFI GGGAC 1 cut(s) 803
BslI CCNNNNNNNGG 6 cut(s) 125, 164, 402, 403, 608, 712
BsmAI GTCTC 2 cut(s) 175, 474
BsmBI CGTCTC 1 cut(s) 175
BsmFI GGGAC 1 cut(s) 803
BsnI GGCC 2 cut(s) 392, 515
BsoBI CYCGRG 1 cut(s) 32
Bsp143I GATC 5 cut(s) 424, 522, 541, 871, 915
BspANI GGCC 2 cut(s) 392, 515
BspCNI CTCAG 3 cut(s) 185, 275, 346
BspFNI CGCG 1 cut(s) 465
BspHI TCATGA 1 cut(s) 874
BspLI GGNNCC 6 cut(s) 56, 91, 247, 493, 543, 600
BspPI GGATC 4 cut(s) 530, 536, 549, 879
BspQI GCTCTTC 1 cut(s) 818
BspT107I GGYRCC 1 cut(s) 245
BsrBI CCGCTC 1 cut(s) 364
BsrDI GCAATG 1 cut(s) 498
BsrFI RCCGGY 1 cut(s) 164
BsrI ACTGG 1 cut(s) 407
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 2 cut(s) 238, 725
BssMI GATC 5 cut(s) 424, 522, 541, 871, 915
BssNAI GTATAC 1 cut(s) 232
BssNI GRCGYC 3 cut(s) 168, 246, 450
Bst1107I GTATAC 1 cut(s) 232
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 1 cut(s) 242
Bst6I CTCTTC 1 cut(s) 818
BstACI GRCGYC 3 cut(s) 168, 246, 450
BstBAI YACGTR 1 cut(s) 651
BstDEI CTNAG 5 cut(s) 112, 172, 262, 354, 912
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 1 cut(s) 451
BstFNI CGCG 1 cut(s) 465
BstH2I RGCGCY 2 cut(s) 249, 725
BstHHI GCGC 4 cut(s) 248, 465, 488, 724
BstKTI GATC 5 cut(s) 427, 525, 544, 874, 918
BstMAI GTCTC 2 cut(s) 175, 474
BstMBI GATC 5 cut(s) 424, 522, 541, 871, 915
BstMWI GCNNNNNNNGC 2 cut(s) 252, 500
BstNI CCWGG 1 cut(s) 207
BstSCI CCNGG 6 cut(s) 51, 106, 205, 537, 560, 724
BstUI CGCG 1 cut(s) 465
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 2 cut(s) 541, 915
BstYI RGATCY 2 cut(s) 541, 915
BstZ17I GTATAC 1 cut(s) 232
BsuRI GGCC 2 cut(s) 392, 515
BtgI CCRYGG 1 cut(s) 238
BtgZI GCGATG 2 cut(s) 307, 702
BtsCI GGATG 1 cut(s) 451
CciI TCATGA 1 cut(s) 874
CfoI GCGC 4 cut(s) 248, 465, 488, 724
Cfr10I RCCGGY 1 cut(s) 164
Cfr13I GGNCC 2 cut(s) 55, 390
CseI GACGC 1 cut(s) 157
Csp6I GTAC 1 cut(s) 358
CviAII CATG 1 cut(s) 875
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 5 cut(s) 112, 172, 262, 354, 912
DinI GGCGCC 1 cut(s) 247
DpnI GATC 5 cut(s) 426, 524, 543, 873, 917
DpnII GATC 5 cut(s) 424, 522, 541, 871, 915
DraIII CACNNNGTG 1 cut(s) 654
Eam1104I CTCTTC 1 cut(s) 818
EarI CTCTTC 1 cut(s) 818
Eco47I GGWCC 1 cut(s) 55
Eco72I CACGTG 1 cut(s) 651
Eco88I CYCGRG 1 cut(s) 32
EcoO109I RGGNCCY 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 205
EgeI GGCGCC 1 cut(s) 247
EheI GGCGCC 1 cut(s) 247
Esp3I CGTCTC 1 cut(s) 175
FaeI CATG 1 cut(s) 878
FaqI GGGAC 1 cut(s) 803
FatI CATG 1 cut(s) 874
FauI CCCGC 4 cut(s) 369, 376, 602, 702
FblI GTMKAC 1 cut(s) 231
Fnu4HI GCNGC 6 cut(s) 6, 267, 372, 393, 418, 685
FokI GGATG 1 cut(s) 458
Fsp4HI GCNGC 6 cut(s) 6, 267, 372, 393, 418, 685
FspBI CTAG 1 cut(s) 224
GlaI GCGC 4 cut(s) 247, 464, 487, 723
GluI GCNGC 6 cut(s) 6, 267, 372, 393, 418, 685
GsaI CCCAGC 1 cut(s) 391
HaeII RGCGCY 2 cut(s) 249, 725
HaeIII GGCC 2 cut(s) 392, 515
HapII CCGG 8 cut(s) 52, 87, 108, 126, 165, 539, 561, 725
HgaI GACGC 1 cut(s) 157
HhaI GCGC 4 cut(s) 248, 465, 488, 724
Hin1I GRCGYC 3 cut(s) 168, 246, 450
Hin1II CATG 1 cut(s) 878
Hin6I GCGC 4 cut(s) 246, 463, 486, 722
HinP1I GCGC 4 cut(s) 246, 463, 486, 722
HinfI GANTC 4 cut(s) 81, 180, 431, 530
HpaII CCGG 8 cut(s) 52, 87, 108, 126, 165, 539, 561, 725
HphI GGTGA 4 cut(s) 9, 187, 640, 832
Hpy166II GTNNAC 2 cut(s) 232, 646
Hpy188I TCNGA 4 cut(s) 479, 535, 546, 676
Hpy188III TCNNGA 5 cut(s) 428, 713, 875, 919, 950
Hpy8I GTNNAC 2 cut(s) 232, 646
Hpy99I CGWCG 2 cut(s) 455, 676
HpyAV CCTTC 3 cut(s) 259, 589, 838
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 2 cut(s) 450, 650
HpyCH4V TGCA 4 cut(s) 8, 255, 308, 503
HpyF10VI GCNNNNNNNGC 2 cut(s) 252, 500
HpyF3I CTNAG 5 cut(s) 112, 172, 262, 354, 912
HpySE526I ACGT 2 cut(s) 450, 650
Hsp92I GRCGYC 3 cut(s) 168, 246, 450
Hsp92II CATG 1 cut(s) 878
HspAI GCGC 4 cut(s) 246, 463, 486, 722
KasI GGCGCC 1 cut(s) 245
Kzo9I GATC 5 cut(s) 424, 522, 541, 871, 915
LguI GCTCTTC 1 cut(s) 818
LmnI GCTCC 5 cut(s) 106, 369, 491, 598, 719
MaeI CTAG 1 cut(s) 224
MaeII ACGT 2 cut(s) 450, 650
MaeIII GTNAC 2 cut(s) 27, 278
MalI GATC 5 cut(s) 426, 524, 543, 873, 917
MbiI CCGCTC 1 cut(s) 364
MboI GATC 5 cut(s) 424, 522, 541, 871, 915
MboII GAAGA 3 cut(s) 392, 434, 835
MfeI CAATTG 2 cut(s) 603, 898
MflI RGATCY 2 cut(s) 541, 915
MluCI AATT 6 cut(s) 337, 555, 603, 658, 815, 898
Mly113I GGCGCC 1 cut(s) 246
MlyI GAGTC 3 cut(s) 75, 174, 524
MmeI TCCRAC 4 cut(s) 457, 558, 654, 711
MseI TTAA 4 cut(s) 411, 576, 891, 945
MspI CCGG 8 cut(s) 52, 87, 108, 126, 165, 539, 561, 725
MspR9I CCNGG 6 cut(s) 53, 108, 207, 539, 562, 726
MunI CAATTG 2 cut(s) 603, 898
MvaI CCWGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 465
MwoI GCNNNNNNNGC 2 cut(s) 252, 500
NarI GGCGCC 1 cut(s) 246
NciI CCSGG 5 cut(s) 53, 108, 539, 562, 726
NdeII GATC 5 cut(s) 424, 522, 541, 871, 915
NlaIII CATG 1 cut(s) 878
NlaIV GGNNCC 6 cut(s) 56, 91, 247, 493, 543, 600
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 1 cut(s) 874
PciSI GCTCTTC 1 cut(s) 818
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PfeI GAWTC 1 cut(s) 431
PflFI GACNNNGTC 1 cut(s) 146
PkrI GCNGC 6 cut(s) 7, 268, 373, 394, 419, 686
PleI GAGTC 3 cut(s) 75, 174, 524
PluTI GGCGCC 1 cut(s) 249
PmaCI CACGTG 1 cut(s) 651
PmlI CACGTG 1 cut(s) 651
PpsI GAGTC 3 cut(s) 75, 174, 524
Ppu21I YACGTR 1 cut(s) 651
PpuMI RGGWCCY 1 cut(s) 55
PshBI ATTAAT 1 cut(s) 945
Psp5II RGGWCCY 1 cut(s) 55
Psp6I CCWGG 1 cut(s) 205
PspCI CACGTG 1 cut(s) 651
PspFI CCCAGC 1 cut(s) 387
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 6 cut(s) 56, 91, 247, 493, 543, 600
PspPI GGNCC 2 cut(s) 55, 390
PspPPI RGGWCCY 1 cut(s) 55
PspXI VCTCGAGB 1 cut(s) 32
PsuI RGATCY 2 cut(s) 541, 915
PsyI GACNNNGTC 1 cut(s) 146
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SapI GCTCTTC 1 cut(s) 818
SaqAI TTAA 4 cut(s) 411, 576, 891, 945
SatI GCNGC 6 cut(s) 6, 267, 372, 393, 418, 685
Sau3AI GATC 5 cut(s) 424, 522, 541, 871, 915
Sau96I GGNCC 2 cut(s) 55, 390
SchI GAGTC 3 cut(s) 75, 174, 524
ScrFI CCNGG 6 cut(s) 53, 108, 207, 539, 562, 726
SetI ASST 9 cut(s) 17, 39, 51, 208, 237, 303, 453, 618, 653
SfoI GGCGCC 1 cut(s) 247
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 1 cut(s) 55
SlaI CTCGAG 1 cut(s) 32
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
Sse9I AATT 6 cut(s) 337, 555, 603, 658, 815, 898
SspDI GGCGCC 1 cut(s) 245
SspMI CTAG 1 cut(s) 224
StyD4I CCNGG 6 cut(s) 51, 106, 205, 537, 560, 724
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 2 cut(s) 453, 653
TaqI TCGA 3 cut(s) 33, 75, 453
TasI AATT 6 cut(s) 337, 555, 603, 658, 815, 898
TauI GCSGC 5 cut(s) 269, 374, 395, 420, 687
TfiI GAWTC 1 cut(s) 431
Tru1I TTAA 4 cut(s) 411, 576, 891, 945
Tru9I TTAA 4 cut(s) 411, 576, 891, 945
TseI GCWGC 1 cut(s) 5
TspDTI ATGAA 3 cut(s) 393, 872, 931
TspGWI ACGGA 3 cut(s) 535, 660, 732
Tth111I GACNNNGTC 1 cut(s) 146
VpaK11BI GGWCC 1 cut(s) 55
VspI ATTAAT 1 cut(s) 945
XapI RAATTY 1 cut(s) 658
XhoI CTCGAG 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 231
XspI CTAG 1 cut(s) 224
ZraI GACGTC 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.