RchiOBHm_Chr6g0307511

carboxylesterase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011899
Physical Location & Seq
Reverse (-)
65850505 .. 65850798
294 bp
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UTR
Exon/CDS
Intron
PRQ27646

Sequence Viewer

Length: 294 bp
ATGCTTGTTGCTGAGGCCAGAGCTATTGCTGTTTCTATTAATTACAGATTAGCCCCTAAGCATCCATTATCGATTGCCTATGAGGATTCTTGGGCAGTACTTCAATGGGTTTTTGGTGGTGAAGATCGTGACGATTGGGTTAAGGATCATGTTGAATTTGAGCAAGCAATGTTCTTGGTGGGAGATAGTGTTGGGCCTAATATTGCACACCACTCGGCCTTGCGGGTCAAGAAGTCCGACCCATCCTTATTTATGGGGGAAAGTTCCGATTGGCCGGGAGGTGGAGAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

97

Amino Acids

10.73

Weight (kDa)

4.83

Isoelectric Point (pI)

31.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 2 - 79 2.2e-20 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 223
AclWI GGATC 1 cut(s) 153
AcoI YGGCCR 1 cut(s) 272
AcsI RAATTY 1 cut(s) 155
AfaI GTAC 1 cut(s) 99
AfiI CCNNNNNNNGG 1 cut(s) 281
AgsI TTSAA 2 cut(s) 104, 155
AluBI AGCT 2 cut(s) 23, 291
AluI AGCT 2 cut(s) 23, 291
AlwI GGATC 1 cut(s) 153
AoxI GGCC 4 cut(s) 15, 194, 216, 272
ApoI RAATTY 1 cut(s) 155
AseI ATTAAT 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 194
AsuC2I CCSGG 1 cut(s) 276
AsuHPI GGTGA 1 cut(s) 131
BbvCI CCTCAGC 1 cut(s) 12
BccI CCATC 1 cut(s) 250
BcgI CGANNNNNNTGC 2 cut(s) 195, 229
BcnI CCSGG 1 cut(s) 276
BmcAI AGTACT 1 cut(s) 99
Bme1390I CCNGG 1 cut(s) 276
BmgT120I GGNCC 1 cut(s) 194
BmrFI CCNGG 1 cut(s) 276
BmsI GCATC 1 cut(s) 70
Bpu10I CCTNAGC 2 cut(s) 12, 57
BpuMI CCSGG 1 cut(s) 276
Bsa29I ATCGAT 1 cut(s) 71
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bsc4I CCNNNNNNNGG 1 cut(s) 281
Bse3DI GCAATG 1 cut(s) 174
BseCI ATCGAT 1 cut(s) 71
BseGI GGATG 2 cut(s) 61, 242
BseLI CCNNNNNNNGG 1 cut(s) 281
BseMI GCAATG 1 cut(s) 174
BshFI GGCC 4 cut(s) 17, 196, 218, 274
BshVI ATCGAT 1 cut(s) 71
BsiSI CCGG 1 cut(s) 275
BslI CCNNNNNNNGG 1 cut(s) 281
BsnI GGCC 4 cut(s) 17, 196, 218, 274
Bsp143I GATC 2 cut(s) 124, 145
BspACI CCGC 1 cut(s) 223
BspANI GGCC 4 cut(s) 17, 196, 218, 274
BspCNI CTCAG 1 cut(s) 4
BspDI ATCGAT 1 cut(s) 71
BspPI GGATC 1 cut(s) 153
BsrDI GCAATG 1 cut(s) 174
BssMI GATC 2 cut(s) 124, 145
BstC8I GCNNGC 1 cut(s) 165
BstDEI CTNAG 2 cut(s) 12, 57
BstF5I GGATG 2 cut(s) 61, 242
BstKTI GATC 2 cut(s) 127, 148
BstMBI GATC 2 cut(s) 124, 145
BstSCI CCNGG 1 cut(s) 274
Bsu15I ATCGAT 1 cut(s) 71
BsuRI GGCC 4 cut(s) 17, 196, 218, 274
BsuTUI ATCGAT 1 cut(s) 71
BtsCI GGATG 2 cut(s) 61, 242
Cac8I GCNNGC 1 cut(s) 165
Cfr13I GGNCC 1 cut(s) 194
ClaI ATCGAT 1 cut(s) 71
Csp6I GTAC 1 cut(s) 98
CviAII CATG 1 cut(s) 149
CviJI RGCY 7 cut(s) 17, 23, 53, 196, 218, 274, 291
CviKI_1 RGCY 7 cut(s) 17, 23, 53, 196, 218, 274, 291
CviQI GTAC 1 cut(s) 98
DdeI CTNAG 2 cut(s) 12, 57
DpnI GATC 2 cut(s) 126, 147
DpnII GATC 2 cut(s) 124, 145
EaeI YGGCCR 1 cut(s) 272
FaeI CATG 1 cut(s) 152
FaiI YATR 3 cut(s) 81, 150, 254
FatI CATG 1 cut(s) 148
FauI CCCGC 1 cut(s) 216
FokI GGATG 2 cut(s) 48, 229
HaeIII GGCC 4 cut(s) 17, 196, 218, 274
HapII CCGG 1 cut(s) 275
Hin1II CATG 1 cut(s) 152
HinfI GANTC 1 cut(s) 86
HpaII CCGG 1 cut(s) 275
HphI GGTGA 1 cut(s) 131
Hpy188I TCNGA 2 cut(s) 238, 268
Hpy188III TCNNGA 2 cut(s) 128, 229
HpyCH4V TGCA 1 cut(s) 206
HpyF3I CTNAG 2 cut(s) 12, 57
Hsp92II CATG 1 cut(s) 152
Kzo9I GATC 2 cut(s) 124, 145
LpnPI CCDG 2 cut(s) 31, 288
LweI GCATC 1 cut(s) 70
MaeIII GTNAC 1 cut(s) 128
MalI GATC 2 cut(s) 126, 147
MboI GATC 2 cut(s) 124, 145
MboII GAAGA 1 cut(s) 134
MluCI AATT 2 cut(s) 40, 155
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 3 cut(s) 7, 76, 272
MseI TTAA 2 cut(s) 39, 141
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 1 cut(s) 276
NciI CCSGG 1 cut(s) 276
NdeII GATC 2 cut(s) 124, 145
NlaIII CATG 1 cut(s) 152
NmeAIII GCCGAG 1 cut(s) 194
NmuCI GTSAC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 86
PshBI ATTAAT 1 cut(s) 39
PspPI GGNCC 1 cut(s) 194
RsaI GTAC 1 cut(s) 99
RsaNI GTAC 1 cut(s) 98
SaqAI TTAA 2 cut(s) 39, 141
Sau3AI GATC 2 cut(s) 124, 145
Sau96I GGNCC 1 cut(s) 194
ScaI AGTACT 1 cut(s) 99
ScrFI CCNGG 1 cut(s) 276
SetI ASST 3 cut(s) 25, 283, 293
SfaNI GCATC 1 cut(s) 70
Sse9I AATT 2 cut(s) 40, 155
SsiI CCGC 1 cut(s) 223
SspI AATATT 1 cut(s) 202
StyD4I CCNGG 1 cut(s) 274
TaqI TCGA 1 cut(s) 71
TasI AATT 2 cut(s) 40, 155
TatI WGTACW 1 cut(s) 97
TfiI GAWTC 1 cut(s) 86
Tru1I TTAA 2 cut(s) 39, 141
Tru9I TTAA 2 cut(s) 39, 141
TseFI GTSAC 1 cut(s) 128
Tsp45I GTSAC 1 cut(s) 128
VspI ATTAAT 1 cut(s) 39
XapI RAATTY 1 cut(s) 155
ZrmI AGTACT 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.