Rorug06G0359200

carboxylesterase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011899
Physical Location & Seq
Forward (+)
50804918 .. 50809394
4477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0359200.1

Sequence Viewer

Length: 1200 bp
ATGGAGCCCTCGTACCTGAACTTGTTCGTGGAGGAGACAAGCTTCTACAACCGCATTGTCCTCGACAGCCTGTTGCCCAACTGGGTGTGGGCCCCGCTGCCCCACTTCTTCCAGTCGTGGCTGCGCAACTACCTCGGCGGCACCTTAATCTACCTCGTCTCCGGCTTCCTCTGGTGCTTCTACATTTACTACCTCAAACGCAACGTCTACCTTCCAAAAGATGCCATTCCCTCTAATAAAGCCATGCTTTTGCAAATATATGTTGCCATGAAGGCCATGCCTTGGTACTGTGCTCTACCGTCTCTCTCTGAGTACATGGTTGAAAATGGCTGGGCGAGGTGCTTCTCAAGAATAAGTGATGTAGGTTGGGTTGCTTACCTCATCTATTTATCAATTTATCTTATAATCGTGGAGTTTGGAATCTATTGGATGCATAGGGAGTTGCATGACATAAAGCCTCTGTATAAATGGCTTCATAATATACATCACATTTACAACAAGCAGAACACCCTCTCTCCATTTGCTGGTTTGGCTTTCCACCCACTTGATGGGATACTGCAGGCAGTTCCGCATGTTATAGCTCTGTTTCTTGTGCCAATGCATTTTACTACTCACATAGCGCTCCTATTTATTGAAGCCATATGGACAGCAAATATTCACGATTGCATCCATGCCAAAATATGGCCTGTGATGGGTGCTGGCTACCACACCATTCATCATACTACCTATCGCCATAATTATGGCCATTATACAATATGGATGGACTGGATGTTTGGAACTCTTCGTGACCCTATCAATGAGGAATCAAAGTTTCGTGGGACACACACCCAATGCATCAGTATAAAAGCAGGAATGGATATCCCAGACAACAAGTCCCTTGAGTACGCCCTTCCATACATACATGGCATAGGTAGAGCCAGGGCTCGCCACATCCTATCTGAGCTCCACATGGACAACAAACTTGCCAGGGACTTGACTAAAAGAGAAATTGTTGCTCTTGGTGATGAACTCTCCAAGTACATAATTGGAAGGGAACTGGCGGCAGTTGTTGAGAAAGACGTTAGACGAATGATAGACATCCAGTCCTACAGAGGGATAAGACATAATGATGGGTTGCCATGCCGAGGACAGCGCACCAAGACCAATGCCCGGACCAGGAAAGCTGGTAAGCGGATTCCTTCTGGAGTGAGGAACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

399

Amino Acids

46.65

Weight (kDa)

9.28

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 132 - 260 1.6e-20 Fatty acid hydroxylase
Ribosomal_S13 PF00416 284 - 385 3.4e-25 Ribosomal protein S13/S18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 404
Acc16I TGCGCA 1 cut(s) 125
AccB1I GGYRCC 1 cut(s) 140
AccB7I CCANNNNNTGG 4 cut(s) 282, 524, 548, 681
AccI GTMKAC 1 cut(s) 207
AciI CCGC 6 cut(s) 52, 95, 138, 569, 1040, 1171
AcoI YGGCCR 1 cut(s) 742
AfaI GTAC 5 cut(s) 14, 287, 314, 884, 1019
AfeI AGCGCT 1 cut(s) 621
AfiI CCNNNNNNNGG 9 cut(s) 282, 524, 548, 681, 692, 1092, 1124, 1149, 1155
AgsI TTSAA 2 cut(s) 323, 635
AhdI GACNNNNNGTC 2 cut(s) 871, 1081
AjnI CCWGG 3 cut(s) 917, 965, 1154
AluBI AGCT 4 cut(s) 42, 581, 943, 1163
AluI AGCT 4 cut(s) 42, 581, 943, 1163
Alw21I GWGCWC 2 cut(s) 295, 945
Alw26I GTCTC 3 cut(s) 29, 163, 306
Aor51HI AGCGCT 1 cut(s) 621
AoxI GGCC 4 cut(s) 90, 273, 683, 742
ApaI GGGCCC 1 cut(s) 94
ApeKI GCWGC 2 cut(s) 97, 121
ArsI GACNNNNNNTTYG 4 cut(s) 189, 221, 755, 787
Asp700I GAANNNNTTC 1 cut(s) 23
AspLEI GCGC 3 cut(s) 126, 622, 1134
AspS9I GGNCC 3 cut(s) 90, 91, 1152
AsuC2I CCSGG 1 cut(s) 1150
AsuHPI GGTGA 1 cut(s) 1013
AvaII GGWCC 1 cut(s) 1152
BaeGI GKGCMC 1 cut(s) 94
BalI TGGCCA 1 cut(s) 744
BanI GGYRCC 1 cut(s) 140
BanII GRGCYC 4 cut(s) 9, 94, 925, 945
Bbv12I GWGCWC 2 cut(s) 295, 945
BbvI GCAGC 2 cut(s) 84, 108
BccI CCATC 4 cut(s) 542, 685, 754, 1103
BcgI CGANNNNNNTGC 4 cut(s) 43, 77, 115, 149
BciT130I CCWGG 3 cut(s) 919, 967, 1156
BciVI GTATCC 1 cut(s) 546
BcnI CCSGG 1 cut(s) 1150
BcoDI GTCTC 3 cut(s) 29, 163, 306
BfmI CTRYAG 2 cut(s) 557, 1087
BfoI RGCGCY 1 cut(s) 623
BfuI GTATCC 1 cut(s) 546
BglI GCCNNNNNGGC 1 cut(s) 272
BisI GCNGC 4 cut(s) 98, 122, 139, 1041
BlsI GCNGC 4 cut(s) 99, 123, 140, 1042
Bme1390I CCNGG 4 cut(s) 919, 967, 1150, 1156
Bme18I GGWCC 1 cut(s) 1152
BmeRI GACNNNNNGTC 2 cut(s) 871, 1081
BmgT120I GGNCC 3 cut(s) 90, 91, 1152
BmiI GGNNCC 4 cut(s) 6, 92, 93, 142
BmrFI CCNGG 4 cut(s) 919, 967, 1150, 1156
BmrI ACTGGG 1 cut(s) 91
BmsI GCATC 4 cut(s) 211, 420, 675, 843
BmuI ACTGGG 1 cut(s) 91
BpuEI CTTGAG 2 cut(s) 331, 899
BpuMI CCSGG 1 cut(s) 1150
BsaBI GATNNNNATC 1 cut(s) 1076
BsaJI CCNNGG 5 cut(s) 133, 281, 918, 966, 1123
BsaXI ACNNNNNCTCC 5 cut(s) 26, 143, 173, 499, 529
Bsc4I CCNNNNNNNGG 9 cut(s) 282, 524, 548, 681, 692, 1092, 1124, 1149, 1155
Bse1I ACTGG 5 cut(s) 86, 112, 770, 1041, 1081
Bse8I GATNNNNATC 1 cut(s) 1076
BseBI CCWGG 3 cut(s) 919, 967, 1156
BseDI CCNNGG 5 cut(s) 133, 281, 918, 966, 1123
BseGI GGATG 6 cut(s) 435, 666, 765, 774, 930, 1077
BseJI GATNNNNATC 1 cut(s) 1076
BseLI CCNNNNNNNGG 9 cut(s) 282, 524, 548, 681, 692, 1092, 1124, 1149, 1155
BseMII CTCAG 2 cut(s) 300, 930
BseNI ACTGG 5 cut(s) 86, 112, 770, 1041, 1081
BseRI GAGGAG 1 cut(s) 47
BseSI GKGCMC 1 cut(s) 94
BseXI GCAGC 2 cut(s) 84, 108
BseYI CCCAGC 1 cut(s) 330
BshFI GGCC 4 cut(s) 92, 275, 685, 744
BshNI GGYRCC 1 cut(s) 140
BsiHKAI GWGCWC 2 cut(s) 295, 945
BsiSI CCGG 2 cut(s) 162, 1150
BslFI GGGAC 3 cut(s) 832, 859, 983
BslI CCNNNNNNNGG 9 cut(s) 282, 524, 548, 681, 692, 1092, 1124, 1149, 1155
BsmAI GTCTC 3 cut(s) 29, 163, 306
BsmBI CGTCTC 2 cut(s) 163, 306
BsmFI GGGAC 3 cut(s) 832, 859, 983
BsnI GGCC 4 cut(s) 92, 275, 685, 744
Bsp120I GGGCCC 1 cut(s) 90
Bsp1286I GDGCHC 5 cut(s) 9, 94, 295, 925, 945
BspACI CCGC 6 cut(s) 52, 95, 138, 569, 1040, 1171
BspANI GGCC 4 cut(s) 92, 275, 685, 744
BspCNI CTCAG 2 cut(s) 301, 931
BspLI GGNNCC 4 cut(s) 6, 92, 93, 142
BspMAI CTGCAG 1 cut(s) 561
BspT107I GGYRCC 1 cut(s) 140
BsrI ACTGG 5 cut(s) 86, 112, 770, 1041, 1081
BssECI CCNNGG 5 cut(s) 133, 281, 918, 966, 1123
BssT1I CCWWGG 1 cut(s) 281
Bst2UI CCWGG 3 cut(s) 919, 967, 1156
Bst4CI ACNGT 2 cut(s) 290, 300
Bst6I CTCTTC 1 cut(s) 786
BstC8I GCNNGC 3 cut(s) 561, 700, 925
BstDEI CTNAG 2 cut(s) 309, 939
BstF5I GGATG 6 cut(s) 435, 666, 765, 774, 930, 1077
BstH2I RGCGCY 1 cut(s) 623
BstHHI GCGC 3 cut(s) 126, 622, 1134
BstMAI GTCTC 3 cut(s) 29, 163, 306
BstMWI GCNNNNNNNGC 2 cut(s) 272, 530
BstNI CCWGG 3 cut(s) 919, 967, 1156
BstNSI RCATGY 1 cut(s) 575
BstSCI CCNGG 4 cut(s) 917, 965, 1148, 1154
BstSFI CTRYAG 2 cut(s) 557, 1087
BstSLI GKGCMC 1 cut(s) 94
BstV1I GCAGC 2 cut(s) 84, 108
BstXI CCANNNNNNTGG 1 cut(s) 740
BsuI GTATCC 1 cut(s) 546
BsuRI GGCC 4 cut(s) 92, 275, 685, 744
BtsCI GGATG 6 cut(s) 435, 666, 765, 774, 930, 1077
Cac8I GCNNGC 3 cut(s) 561, 700, 925
CfoI GCGC 3 cut(s) 126, 622, 1134
Cfr13I GGNCC 3 cut(s) 90, 91, 1152
Csp6I GTAC 5 cut(s) 13, 286, 313, 883, 1018
CviQI GTAC 5 cut(s) 13, 286, 313, 883, 1018
DdeI CTNAG 2 cut(s) 309, 939
DriI GACNNNNNGTC 2 cut(s) 871, 1081
EaeI YGGCCR 1 cut(s) 742
Eam1104I CTCTTC 1 cut(s) 786
Eam1105I GACNNNNNGTC 2 cut(s) 871, 1081
EarI CTCTTC 1 cut(s) 786
Ecl136II GAGCTC 1 cut(s) 943
Eco130I CCWWGG 1 cut(s) 281
Eco24I GRGCYC 4 cut(s) 9, 94, 925, 945
Eco32I GATATC 1 cut(s) 859
Eco47I GGWCC 1 cut(s) 1152
Eco47III AGCGCT 1 cut(s) 621
Eco53kI GAGCTC 1 cut(s) 943
EcoICRI GAGCTC 1 cut(s) 943
EcoO109I RGGNCCY 1 cut(s) 91
EcoRII CCWGG 3 cut(s) 917, 965, 1154
EcoRV GATATC 1 cut(s) 859
EcoT14I CCWWGG 1 cut(s) 281
EcoT22I ATGCAT 3 cut(s) 435, 603, 836
EcoT38I GRGCYC 4 cut(s) 9, 94, 925, 945
ErhI CCWWGG 1 cut(s) 281
Esp3I CGTCTC 2 cut(s) 163, 306
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FaqI GGGAC 3 cut(s) 832, 859, 983
FauI CCCGC 1 cut(s) 102
FauNDI CATATG 1 cut(s) 641
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 4 cut(s) 98, 122, 139, 1041
FokI GGATG 6 cut(s) 442, 653, 772, 781, 917, 1064
FriOI GRGCYC 4 cut(s) 9, 94, 925, 945
Fsp4HI GCNGC 4 cut(s) 98, 122, 139, 1041
FspI TGCGCA 1 cut(s) 125
GlaI GCGC 3 cut(s) 125, 621, 1133
GluI GCNGC 4 cut(s) 98, 122, 139, 1041
GsaI CCCAGC 1 cut(s) 334
HaeII RGCGCY 1 cut(s) 623
HaeIII GGCC 4 cut(s) 92, 275, 685, 744
HapII CCGG 2 cut(s) 162, 1150
HhaI GCGC 3 cut(s) 126, 622, 1134
Hin6I GCGC 3 cut(s) 124, 620, 1132
HinP1I GCGC 3 cut(s) 124, 620, 1132
HindIII AAGCTT 1 cut(s) 40
HinfI GANTC 3 cut(s) 420, 803, 1174
HpaII CCGG 2 cut(s) 162, 1150
HphI GGTGA 1 cut(s) 1013
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 2 cut(s) 310, 940
Hpy188III TCNNGA 4 cut(s) 348, 659, 785, 1182
Hpy8I GTNNAC 1 cut(s) 208
HpyAV CCTTC 5 cut(s) 221, 265, 899, 1023, 1188
HpyCH4III ACNGT 2 cut(s) 290, 300
HpyCH4IV ACGT 2 cut(s) 204, 1059
HpyCH4V TGCA 7 cut(s) 253, 433, 445, 559, 601, 666, 834
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 530
HpyF3I CTNAG 2 cut(s) 309, 939
HpySE526I ACGT 2 cut(s) 204, 1059
HspAI GCGC 3 cut(s) 124, 620, 1132
LmnI GCTCC 3 cut(s) 4, 627, 948
Lsp1109I GCAGC 2 cut(s) 84, 108
LweI GCATC 4 cut(s) 211, 420, 675, 843
MaeII ACGT 2 cut(s) 204, 1059
MaeIII GTNAC 1 cut(s) 785
MboII GAAGA 2 cut(s) 100, 773
MhlI GDGCHC 5 cut(s) 9, 94, 295, 925, 945
MlsI TGGCCA 1 cut(s) 744
MluCI AATT 4 cut(s) 393, 736, 987, 1023
MluNI TGGCCA 1 cut(s) 744
Mox20I TGGCCA 1 cut(s) 744
Mph1103I ATGCAT 3 cut(s) 435, 603, 836
MroXI GAANNNNTTC 1 cut(s) 23
MscI TGGCCA 1 cut(s) 744
MseI TTAA 1 cut(s) 146
MslI CAYNNNNRTG 2 cut(s) 738, 1107
Msp20I TGGCCA 1 cut(s) 744
MspA1I CMGCKG 1 cut(s) 97
MspI CCGG 2 cut(s) 162, 1150
MspR9I CCNGG 4 cut(s) 919, 967, 1150, 1156
MvaI CCWGG 3 cut(s) 919, 967, 1156
MwoI GCNNNNNNNGC 2 cut(s) 272, 530
NciI CCSGG 1 cut(s) 1150
NdeI CATATG 1 cut(s) 641
NlaIV GGNNCC 4 cut(s) 6, 92, 93, 142
NmeAIII GCCGAG 2 cut(s) 114, 1148
NmuCI GTSAC 1 cut(s) 785
NsbI TGCGCA 1 cut(s) 125
NsiI ATGCAT 3 cut(s) 435, 603, 836
NspI RCATGY 1 cut(s) 575
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 3 cut(s) 420, 803, 1174
PflMI CCANNNNNTGG 4 cut(s) 282, 524, 548, 681
PkrI GCNGC 4 cut(s) 99, 123, 140, 1042
PsiI TTATAA 1 cut(s) 404
Psp124BI GAGCTC 1 cut(s) 945
Psp6I CCWGG 3 cut(s) 917, 965, 1154
PspFI CCCAGC 1 cut(s) 330
PspGI CCWGG 3 cut(s) 917, 965, 1154
PspN4I GGNNCC 4 cut(s) 6, 92, 93, 142
PspOMI GGGCCC 1 cut(s) 90
PspPI GGNCC 3 cut(s) 90, 91, 1152
PstI CTGCAG 1 cut(s) 561
RsaI GTAC 5 cut(s) 14, 287, 314, 884, 1019
RsaNI GTAC 5 cut(s) 13, 286, 313, 883, 1018
RseI CAYNNNNRTG 2 cut(s) 738, 1107
SacI GAGCTC 1 cut(s) 945
SaqAI TTAA 1 cut(s) 146
SatI GCNGC 4 cut(s) 98, 122, 139, 1041
Sau96I GGNCC 3 cut(s) 90, 91, 1152
ScrFI CCNGG 4 cut(s) 919, 967, 1150, 1156
SduI GDGCHC 5 cut(s) 9, 94, 295, 925, 945
SfaNI GCATC 4 cut(s) 211, 420, 675, 843
SfcI CTRYAG 2 cut(s) 557, 1087
SinI GGWCC 1 cut(s) 1152
SmiMI CAYNNNNRTG 2 cut(s) 738, 1107
SmlI CTYRAG 2 cut(s) 346, 878
SmoI CTYRAG 2 cut(s) 346, 878
Sse9I AATT 4 cut(s) 393, 736, 987, 1023
SsiI CCGC 6 cut(s) 52, 95, 138, 569, 1040, 1171
SspI AATATT 1 cut(s) 655
SstI GAGCTC 1 cut(s) 945
StyD4I CCNGG 4 cut(s) 917, 965, 1148, 1154
StyI CCWWGG 1 cut(s) 281
TaaI ACNGT 2 cut(s) 290, 300
TaiI ACGT 2 cut(s) 207, 1062
TaqI TCGA 1 cut(s) 63
TasI AATT 4 cut(s) 393, 736, 987, 1023
TatI WGTACW 2 cut(s) 312, 1017
TauI GCSGC 2 cut(s) 141, 1043
TfiI GAWTC 3 cut(s) 420, 803, 1174
Tru1I TTAA 1 cut(s) 146
Tru9I TTAA 1 cut(s) 146
TseFI GTSAC 1 cut(s) 785
TseI GCWGC 2 cut(s) 97, 121
Tsp45I GTSAC 1 cut(s) 785
TspDTI ATGAA 4 cut(s) 284, 464, 704, 1020
Van91I CCANNNNNTGG 4 cut(s) 282, 524, 548, 681
VpaK11BI GGWCC 1 cut(s) 1152
XceI RCATGY 1 cut(s) 575
XcmI CCANNNNNNNNNTGG 1 cut(s) 545
XmiI GTMKAC 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 23
Zsp2I ATGCAT 3 cut(s) 435, 603, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.